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| 1 | Inactivation of SACE_3446, a TetR family transcriptional regulator, stimulates erythromycin production in Saccharopolyspora erythraea显示文摘Erythromycin A is a widely used antibiotic produced by Saccharopolyspora erythraea;however,its biosynthetic cluster lacks a regulatory gene,limiting the yield enhancement via regulation engineering of S.erythraea.Herein,six TetR family transcriptional regulators(TFRs)belonging to three genomic context types were individually inactivated in S.erythraea A226,and one of them,SACE_3446,was proved to play a negative role in regulating erythromycin biosynthesis.EMSA and qRT-PCR analysis revealed that SACE_3446 covering intact N-terminal DNA binding domain specifically bound to the promoter regions of erythromycin biosynthetic gene eryAI,the resistant gene ermE and the adjacent gene SACE_3447(encoding a longchain fatty-acid CoA ligase),and repressed their transcription.Furthermore,we explored the interaction relationships of SACE_3446 and previously identified TFRs(SACE_3986 and SACE_7301)associated with erythromycin production.Given demonstrated relatively independent regulation mode of SACE_3446 and SACE_3986 in erythromycin biosynthesis,we individually and concomitantly inactivated them in an industrial S.erythraea WB.Compared with WB,the WBΔ3446 and WBΔ3446Δ3986 mutants respectively displayed 36%and 65%yield enhancement of erythromycin A,following significantly elevated transcription of eryAI and ermE.When cultured in a 5 L fermentor,erythromycin A ofWBΔ3446 and WBΔ3446Δ3986 successively reached 4095 mg/L and 4670 mg/L with 23%and 41%production improvement relative to WB.The strategy reported here will be useful to improve antibiotics production in other industrial actinomycete. | Hang Wu Yansheng Wang Li Yuan Yongrong Mao Weiwei Wang Lin Zhu Panpan Wu Chengzhang Fu Rolf Muller David T.Weaver Lixin Zhang Buchang Zhang | 2016 | Synthetic and Systems Biotechnology2016，1，1： | 7 |
| 2 | Tissue engineering using 3D printed nano-bioactive glass loaded with NELL1 gene for repairing alveolar bone defects显示文摘The purposes of this study were to construct a novel tissue engineered bone composed of 3Dprinted bioactive glass block/chitosan nanoparticles(BD/CSn)composites loaded with Nel-like Type I molecular-1 DNA(pDNA-NELL1)and/or bone marrow mesenchymal stem cells(BMSCs),and study their osteogenic activities by repairing bone defects in rhesus monkeys.CSn with NELL1 gene plasmid and rhesus monkey BMSCs were composited with a BD scaffold to prepare the tissue-engineered bone.Four adult female rhesus monkeys with 10-to 12-years old and 5-7 kg in weight were used in animal experiments.The first and second premolar teeth from four regions of each monkey were removed to form bone defects with size of 10×10×5 mm,which were then implanted with above-mentioned tissue engineered bone.At 12 weeks after the implantation,gross observations,X-ray and micro-CT observations revealed that the new bone was extremely close to normal bone in mass,density,hardness,and structure.The bony cortex was smooth and closely connected to the surrounding normal bone.Histological observations revealed moderate inflammation in the repair area,and the new bone tissues were similar to normal ones.In conclusion,tissue engineered bone of this study exhibited good osteoconductivity for promoting the formation of new alveolar bone tissue,and NELL1 gene played a promotional role in bone regeneration. | Jing Zhang Yang Chen Jing Xu Jingjing Wang Chengzhang Li Liyan Wang | 2018 | Regenerative Biomaterials2018，5，4： | 6 |
| 3 | Catalytic effect of Ni@rGO on the hydrogen storage properties of MgH2显示文摘Uniform-uispersed Ni nanoparticics(NPs)anchored on reduced graphene oxide(Ni@rGO)catalyzed MgH2(MH-Ni@rGO)has been fabricated by mechanical milling.The effects of milling time and Ni loading amount on the hydrogen storage properties of MgH2 have been investigated.The initial hydrogen desorption temperature of MgH2 catalyzed by 10 wt.%Ni4@rGO6 for milling 5 h is significantly decreased from 251℃ to 190℃.The composite can absorb 5.0 wt.%hydrogen in 20 min at 100℃,while it can desorb 6.1 wt.%within 15 min at 300℃.Through the investigation of the phase transformation and dehydrogenation kinetics during hydrogen ab/desorption cycles,we found that the in-situ formed Mg2Ni/Mg2NiH4 exhibited better catalytic effect than Ni.When Ni loading amount is 45 wt.%,the rGO in Ni@rGO catalysts can prevent the reaction of Ni and Mg due to the strong interaction between rGO and Ni NPs. | Pengyang Yao Ying Jiang Yang Liu Chengzhang Wu Kuo-Chih Chou Tao Lyu Qian Li | 2020 | Journal of Magnesium and Alloys2020，8，2： | 4 |
| 4 | Ultrathin wavy Rh nanowires as highly effective electrocatalysts for methanol oxidation reaction with ultrahigh ECSA显示文摘Direct methanol fuel cells (DMFCs) have received tremendous research interests because of the facile storage of liquid methanol vs.hydrogen.However,the DMFC today is severely plagued by the poor kinetics and rather high overpotential in methanol oxidation reaction (MOR).Here we report the investigation of the ultrathin Rh wavy nanowires as a highly effective MOR electrocatalyst.We show that ultrathin wavy Rh nanowires can be robustly synthesized with 2-3 nm diameters.Electrochemical studies show a current peak at the potential of 0.61 V vs.reversible hydrogen electrode (RHE),considerably lower than that of Pt based catalysts (~ 0.8-0.9 V vs.RHE).Importantly,with ultrathin diameters and favorable charge transport,the Rh nanowires catalysts exhibit an ultrahigh electrochemically active surface area determined from CO-stripping (ECSAco) of 144.2 m2/g,far exceeding that of the commercial Rh black samples (20 m2/g).Together,the Rh nanowire catalysts deliver a mass activity of 722 mA/mg at 0.61 V,considerably higher than many previously reported electrocatalysts at the same potential.The chronoamperometry studies also demonstrate good stability and CO-tolerance compared with the Rh black control sample,making ultrathin Rh wavy nanowires an attractive electrocatalyst for MOR. | Xiaoyang Fu Zipeng Zhao Chengzhang Wan Yiliu Wang Zheng Fan Frank Song Bocheng Cao Mufan Li Wang Xue Yu Huang Xiangfeng Duan | 2019 | Nano Research2019，12，1： | 3 |
| 5 | Preparation of composite poly(ether block amide)membrane for CO_2 capture显示文摘In this study, a poly(ether block amide)(Pebax 1657) composite membrane applied for CO2 capture was prepared by coating Pebax 1657 solution on polyacrylonitrile(PAN) ultrafiltration membrane. Ethanol/water mixture was used as the solvent of Pebax and the effects of ethanol/water mass ratios and Pebax concentration on the permeation properties of composite membrane were studied. To enhance the composite membrane permeance, the gutter layer, made from reactive amino silicone crosslinking with polydimethylsiloxane(PDMS), was designed. The influence of crosslinking degree of the gutter layer on membrane performance was investigated. As a result, a Pebax/aminoPDMS/PAN multilayer membrane with hexane resistance was developed, showing CO2 permeance of 350 GPU and CO2/N2 selectivity over50. The blend of polyethylene glycol dimethyl ether(PEG-DME) with Pebax as coating material was studied to further improve the membrane performance. After being combined with PEG-DME additive, CO2 permeance of the final Pebax-PEG-DME/amino-PDMS/PAN composite membrane reached 400 GPU above with CO2/N2 selectivity over 65. | Lianjun Wang Yang Li Shuguang Li Pengfei Ji Chengzhang Jiang | 2014 | Journal of Energy Chemistry2014，23，6： | 3 |
| 6 | Invasion of Spartina alterniflora Enhanced Ecosystem Carbon and Nitrogen Stocks in the Yangtze Estuary, China显示文摘 | Chengzhang Liao Yiqi Luo Lifen Jiang Xuhui Zhou Xiaowen Wu Changming Fang Jiakuan Chen Bo Li | 2007 | Ecosystems2007，，： | 1 |
| 7 | Defense Against Poisoning Attack via Evaluating TrainingSamples Using Multiple Spectral Clustering Aggregation Method显示文摘The defense techniques for machine learning are critical yet challenging due tothe number and type of attacks for widely applied machine learning algorithms aresignificantly increasing. Among these attacks, the poisoning attack, which disturbsmachine learning algorithms by injecting poisoning samples, is an attack with the greatestthreat. In this paper, we focus on analyzing the characteristics of positioning samples andpropose a novel sample evaluation method to defend against the poisoning attack cateringfor the characteristics of poisoning samples. To capture the intrinsic data characteristicsfrom heterogeneous aspects, we first evaluate training data by multiple criteria, each ofwhich is reformulated from a spectral clustering. Then, we integrate the multipleevaluation scores generated by the multiple criteria through the proposed multiplespectral clustering aggregation (MSCA) method. Finally, we use the unified score as theindicator of poisoning attack samples. Experimental results on intrusion detection datasets show that MSCA significantly outperforms the K-means outlier detection in terms ofdata legality evaluation and poisoning attack detection. | Wentao Zhao Pan Li Chengzhang Zhu Dan Liu Xiao Liu | 2019 | Computers, Materials & Continua2019，，6： | 1 |
| 8 | Easy replication of pueraria lobata toward hierarchically ordered porous 7-Al2O3显示文摘 | Li Chengzhang He Junhui | 2006 | Langmuir2006，22，6： | 1 |
| 9 | Spartina alterniflora invasions in the Yangtze River estuary, China: An overview of current status and ecosystem effects 显示文摘 | LI Bo LIAO Chengzhang ZHANG Xiaodong | 2009 | Ecological Engineering2009，35，4： | 1 |
| 10 | Easy replication of pueraria lobata toward hierarchically ordered porous γ-Al2O3显示文摘 | Chengzhang Li Junhui He | 2006 | Langmuir2006，22，： | 1 |
| 11 | Rose Bengal staining in detection of oral precancerous and malignant lesions with colorimetric evaluation: a pilot study 显示文摘 | Du Gefei Li Chengzhang Chen Hanzhang | 2007 | International Journal of Cancer2007，120，9： | 1 |
| 12 | Recent progress of iron-based electrocatalysts for nitrogen reduction reaction显示文摘Ammonia is a critical feedstock for modern industry and agriculture,which is also a promising carbonfree energy carrier.Electrochemical nitrogen reduction reaction(ENRR)is a sustainable and efficient approach to synthesize ammonia under ambient reaction conditions.ENRR relies on the development of highly efficient electrocatalysts.So far various strategies have been applied to enhance the performance of catalysts including tuning the electronic structure,binding strength,coordination configuration as well as the introduction of multiple active sites and defects.In this review,the recent progress of Fe-based electrocatalysts and their coordination effect,syner gistic effect and def ect effect for nitrogen reduction reaction have been summarized.Our critical review focuses on the discussion of theoretical advancement,the performance origin of ENRR activity and selectivity of Fe-based electrocatalysts. | Tianyi Wang Zhongyuan Guo Xiaoli Zhang Qinye Li Aimin Yu Chengzhang Wu Chenghua Sun | 2023 | Journal of Materials Science & Technology2023，，9： | 0 |
| 13 | Effects of aerosolized ketamine on the level of nitric oxide and nitric oxide synthetase in the lung tissue of rat with asthma显示文摘Objective: To explore the effects of aerosolized ketamine on the level of nitric oxide and nitric oxide synthetase in the lung tissue in rat asthma model. Methods: Forty SD rats were randomly assigned to five groups: control group (group N), asthma model group (group A), two pretreated groups of different concentrations of ketamine (group K1, K2) and dexamethasone group(group D) with eight rats in each group.The rats in group A were sensitized by injection of ovalbumin (OA) together with aluminum hydroxide and bordetella pertussis as adjuvants. Two weeks after the sensitization,aerosolized OA was used to cause asthma. The rats in group K1 and K2 were sensitized with OA as group A , and then exposed to aerosol of ketamine ,with the concentration of 25 g/L and 50 g/L respectively.Before using aerosolized OA,the rats in group D were exposed to aerosol of 0.01% dexamethasone .The level of NO2-/NO3- in lung tissues, inducible nitric oxide synthetase(iNOS) and constitute nitric oxide synthetase(cNOS) was measured in all groups. Results: The level of NO2-/NO3- and the activity of iNOS in lung tissues in group A were signiticantly higher than those in the other groups. The iNOS activity and the level of NO2-/NO3- in lung tissues were highly positively correlated. Conclusion: NO can induce airway hyperreactivity that may worsen asthma. Aerosolized ketamine can decrease the iNOS expression and reduce the level of NO in the lung tissue in rat asthma model. | Weiqian Tian Chengzhang Fu Minmin Zhu Haixia Li | 2006 | Journal of Nanjing Medical University2006，20，2： | 0 |
| 14 | Potential application of proteolysis targeting chimera(PROTAC)modification technology in natural products for their targeted protein degradation显示文摘There are numerous evaluations of natural products,of which majority are food bioactives,performed up to date for their various health beneficial activities via targeting specific proteins.However,the direct identification of a targeted protein remains unexplored for natural occurring compounds.Proteolysis targeting chimera(PROTAC)is a type of bifunctional chimeric molecules that can directly degrade the binding proteins targeted by bioactive molecules in an ubiquitin-proteasome pathway.As the agents in protein degradation dependent on ubiquitin ligase,the bifunctional molecule connects the target protein ligand and E3 ligase ligand together via an appropriate linker.It is highly selective and efficient to induce the ubiquitin-mediated degradation of targeted binding proteins.Therefore,it has been demonstrated that the PROTAC technology has broad application in the modulation of the target protein level.In this review,we outlined the advances in PROTAC combined molecule compounds,summarized its quantitative structure-activity relationship,and finally reviewed the methods applied in identifying the target proteins of natural products.We hope it will provide an insightful application of PROTAC techniques in the target protein identification of natural products including food bioactive molecules. | Guliang Yang Haiyan Zhong Xinxin Xia Zhiwen Qi Chengzhang Wang Shiming Li | 2022 | Food Science and Human Wellness2022，11，2： | 0 |
| 15 | Thoughts on the Sustainable Development of the Fava Bean Industry in Chongqing显示文摘The research analyzed the theoretical basis of the sustainable development of the Chongqing fava bean in terms of the current market economy situation. In regard to practice, several strategies will be suggested for the Chongqing fava bean industry to guide the development of Chongqing's efficient fava bean agriculture. | Chengzhang DU Yanghua LI Jijun ZHANG | 2015 | Agricultural Science & Technology2015，16，6： | 0 |
| 16 | The Modified GM( 1 , 1) Grey Forecast Model显示文摘TheModifiedGM(1,1)GreyForecastModelWangChengzhang;GuoYaohuang;LiQiang(SchoolofEconomicsandManagement,SouthwestJiaotongUnivers... | Wang Chengzhang Guo Yaohuang Li Qiang (School of Economics and Management,Southwest Jiaotong University)Chengdu 61 0031 , China | 1995 | Journal of Modern Transportation1995，12，2： | 0 |
| 17 | The hydrogen storage performance and catalytic mechanism of theMgH_(2)-MoS_(2)composite显示文摘In this work,we synthesized MoS_(2)catalyst via one-step hydrothermal method,and systematically investigated the catalytic effect of MoS_(2)on the hydrogen storage properties of MgH_(2).The MgH_(2)-5MoS_(2)composite milled for 5 h starts to release hydrogen at 259℃.Furthermore,it can desorb 4.0 wt.%hydrogen within 20 min at 280℃,and absorb 4.5 wt.%hydrogen within 5 min at 200℃.Mo and MoS_(2)coexistedin the ball milled sample,whereas only Mo was kept in the sample after dehydrogenation and rehydrogenation,which greatly weakens theMg-H bonds and facilitates the dissociation of MgH_(2)on the surface of Mo(110).The comparative study show that the formed MgS has nocatalytic effect for MgH_(2).We believed that the evolution and the catalytic mechanism of MoS_(2)will provide the theoretical guidance for theapplication of metal sulfide in hydrogen storage materials. | Luxiang Wang Yiwanting Hu Jiayu Lin Haiyan Leng Chenghua Sun Chengzhang Wu Qian Li Fusheng Pan | 2023 | Journal of Magnesium and Alloys2023，11，7： | 0 |
| 18 | J-shaped association between dietary zinc intake and new-onset hypertension:a nationwide cohort study in China显示文摘We aimed to investigate the relationship of dietary zinc intake with new-onset hypertension among Chinese adults.A total of 12,177 participants who were free of hypertension at baseline from the China Health and Nutrition Survey were included.Dietary intake was assessed by three consecutive 24-h dietary recalls combined with a household food inventory.Participants with systolic blood pressure≥140 mmHg or diastolic blood pressure≥90 mmHg or diagnosed by a physician or under antihypertensive treatment during the follow-up were defined as having new-onset hypertension.During a median follow-up duration of 6.1 years,4269 participants developed new-onset hypertension.Overall,the association between dietary zinc intake and new-onset hypertension followed a J-shape(P for non-linearity<0.001).The risk of new-onset hypertension significantly decreased with the increment of dietary zinc intake(per mg/day:hazard ratio(HR)0.93;95%confidence interval(CI)0.88–0.98)in participants with zinc intake<10.9 mg/day,and increased with the increment of zinc intake(per mg/day:HR 1.14;95%CI 1.11–1.16)in participants with zinc intake≥10.9 mg/day.In conclusion,there was a J-shaped association between dietary zinc intake and new-onset hypertension in general Chinese adults,with an inflection point at about 10.9 mg/day. | Panpan He Huan Li Mengyi Liu Zhuxian Zhang Yuanyuan Zhang Chun Zhou Ziliang Ye Qimeng Wu Min Liang Jianping Jiang Guobao Wang Jing Nie Fan Fan Hou Chengzhang Liu Xianhui Qin | 2023 | Frontiers of Medicine2023，17，1： | 0 |
| 19 | Looking into the Root Causes of Power Shortage显示文摘The year 2011-another power-shortage year is gone.It is a year seeing a different power shortage from those in the previous ones.It is a power-shortage year full of conflicts and lack of wide understanding. 'Soft power shortage' is a new phrase,meaning that power shortage emerges under the condition of adequate installed capacity.At present,the power generation companies in China are in a very difficult time,suffering great losses because of high coal price and short coal supply.Coal-fired units shutdown plus sharp hydro generation decline in spring resulted in serious power shortage.Why did this happen? Let's analyze the root causes of electricity shortage in 2011. | Zhu Chengzhang Zhu Li | 2012 | Electricity2012，23，3： | 0 |
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