|
|
|
题名
|
作者
|
年代
|
出处
|
被引量
|
| 1 | Purification and Characterization of a Mannose-binding Lectin from the Rhizomes of Aspidistra elatior Blume with Antiproliferative Activity显示文摘有一个新奇 N 终端氨基酸序列的凝集素被硫酸铵降水,离子交换层析 ondiethylaminoethyl-Sepharose 和 carboxymethyl-Sepharose 和凝胶过滤层析 onSephacryl S-100 从蜘蛛抱蛋 elatior 布鲁坶的根茎净化。A。elatior 布鲁坶凝集素(AEL ) 是有 56kDa 的一个分子的团的 heterotetramer 并且一起由 13.5 kDa 和 14.5 kDaheld 的二不同多肽组成由的二 homodimers 创作非共有原子价相互作用。Hapten 抑制试金显示向兔子红血球的 AEL 的那项 hemagglutinating 活动能被 D 甘露糖禁止,甘露聚糖, thyroglobulinand ovomucoid。凝集素是稳定的直到 70 ° C,并且在 7.0-8.0 的一个狭窄的 pH 范围显示出最大的活动。化学修正和光谱分析显示色氨酸,精氨酸,半胱氨酸和 carboxyl 组织残余为它的 hemagglutinating 活动是必要的。然而,他们可能不在活跃中心是在场的,除了一些 carboxyl 组残余。AEL 也出现了在向 Bre-04 (66%) 的 antiproliferative 活动, Lu-04 (60%) 和人的癌症房间的 HepG2 (56%) 衬里的 vitro 重要。 | Xiaochao XU Chuanfang WU Chao LIU Yongting LUO Jian LI Xinping ZHAO Els Van DAMME Jinku BAO | 2007 | Acta Biochimica et Biophysica Sinica2007，39，7： | 8 |
| 2 | Coagulation factors VII, IX and X are effective antibacterial proteins against drug-resistant Gram-negative bacteria显示文摘Infections caused by drug-resistant“superbugs”pose an urgent public health threat due to the lack of effective drugs;however, certain mammalian proteins with intrinsic antibacterial activity might be underappreciated. Here, we reveal an antibacterial property against Gram-negative bacteria for factors VII, IX and X, three proteins with well-established roles in initiation of the coagulation cascade. These factors exert antibacterial function via their light chains (LCs). Unlike many antibacterial agents that target cell metabolism or the cytoplasmic membrane, the LCs act by hydrolyzing the major components of bacterial outer membrane, lipopolysaccharides, which are crucial for the survival of Gram-negative bacteria. The LC of factor VII exhibits in vitro efficacy towards all Gram-negative bacteria tested, including extensively drug-resistant (XDR) pathogens, at nanomolar concentrations. It is also highly effective in combating XDR Pseudomonas aeruginosa and Acinetobacter baumannii infections in vivo. Through decoding a unique mechanism whereby factors VII, IX and X behave as antimicrobial proteins, this study advances our understanding of the coagulation system in host defense, and suggests that these factors may participate in the pathogenesis of coagulation disorder-related diseases such as sepsis via their dual functions in blood coagulation and resistance to infection. Furthermore, this study may offer new strategies for combating Gram-negative “superbugs”. | Jinwu Chen Xiaojie Li Ling Li Ting Zhang Qing Zhang Fangming Wu Diyue Wang Hongze Hu Changlin Tian Dongsheng Liao Liang Zhao Danxia Song Yongyun Zhao Chuanfang Wu Xu Song | 2019 | Cell Research2019，29，9： | 6 |
| 3 | Dynamic Model and Motion Mechanism of Magnetotactic Bacteria with Two Lateral Flagellar Bundles显示文摘 | Cenyu Yang Chuanfang Chen Qiufeng Ma Longfei Wu Tao Song | 2012 | Journal of Bionic Engineering2012，9，2： | 4 |
| 4 | Conformational study reveals amino acid residues essential for hemagglutinating and anti-proliferative activities of Clematis montana lectin显示文摘铁线莲 montana lectin (电流型逻辑) ,从 C 净化的新奇甘露糖绑定 lectin。montana Buch 火腿茎(Ranunculaceae ) ,被证明了在肿瘤房间在兔子红血球和导致 apoptosis 活动举办 hemagglutinating 活动。然而,电流型逻辑的生物化学的性质没揭示,它的结构的信息仍然需要被阐明。在这研究,电流型逻辑拥有了相当好的 thermostability 和碱的抵抗,这被发现,并且它的 hemagglutinating 活动是二原子价的金属阳离子依赖者。另外,红血球凝聚测试和荧光光谱学证明了硫酸盐能改变的那 GuHCl,脲,和钠 dodecyl 是电流型逻辑的符合构造并且进一步引起了红血球凝聚活动的损失。而且,荧光光谱的变化显示色氨酸(Trp ) 微型环境变换可能与电流型逻辑的符合构造和 bioactivities 有关。另外, Trp 残余,精氨酸(Arg ) 残余,和 sulfhydryl 为电流型逻辑的 hemagglutinating 活动是重要的,这也被发现,但是仅仅 Trp 被证明为电流型逻辑符合构造关键。而且, Trp, Arg,和修改 sulfhydryl 的电流型逻辑展出了 97.17% , 76.99% ,和 49.64% 它的 anti-proliferative 活动的损失,分别地它与它的 hemagglutinating 活动的改变一致。给这些调查结果,电流型逻辑的表面上的 Trp 残余是必要的让活跃中心为糖类绑定形成底层可存取的符合构造和合适的环境。 | Bangmin Lu Bin Zhang Wei Qi Yanan Zhu Yan Zhao Nan Zhou Rong Sun Jinku Bao Chuanfang Wu | 2014 | Acta Biochimica et Biophysica Sinica2014，46，11： | 2 |
| 5 | A novel mannose-binding lectin from Liparis nervosa with anti-fungal and anti-tumor activities显示文摘Plant lectins are carbohydrate-binding proteins with nonimmune origin,which can reversibly bind with carbohydrates,agglutinate cells,and precipitate polysaccharides and glycoconjugates.Plant lectins have attracted much attention for their anti-virus,anti-proliferation,and pro-apoptosis properties.Thus the exploration of new lectins has received special attention.Here we purified a mannose-binding lectin from the rhizomes of Liparis nervosa by ion exchange chromatography on DEAE-Sepharose,affinity chromatography on Mannose-Sepharose 4B,and gel filtration chromatography on Sephacryl S-100.The purified L.nervosa lectin(LNL)was identified to be a monomeric protein with a molecular mass of 13 kDa.LNL exhibited hemagglutinating activity towards rabbit erythrocytes,and its activity could be strongly inhibited by D-mannose,N-acetyl glucosamine and thyroglobulin.In vitro experiments showed that LNL exhibited a comparable anti-fungal activity against Piricularia oryzae(Cavara),Bipolaris maydis,Fusarium graminearum,and Sclerotium rolfsii,and anti-proliferation activity against tumor cells by inducing apoptosis.The full-length cDNA sequence of LNL is 715 bp in length and contains a 525 bp open reading frame(ORF)encoding a 110-residue mature protein.It was predicted to have three mannose-binding conserved motifs‘QXDXNXVXY’.The binding pattern of LNL was further revealed by homology modeling and molecular docking.We demonstrated that LNL is not only a potential therapeutic candidate against tumor but also a new anti-fungal agent. | Na Jiang Yuqing Wang Jing Zhou Ruxiao Zheng Xiao Yuan Miaomiao Wu Jinku Bao Chuanfang Wu | 2020 | Acta Biochimica et Biophysica Sinica2020，52，10： | 1 |
| 6 | Human eukaryotic elongation factor 1A forms oligomers through specific cysteine residues显示文摘真核细胞的延伸因素 1A (eEF1A ) 是涉及捆绑肌动朊的多功能的蛋白质,切断微导管,激活 phosphoinositol-4 kinase,并且招募 aminoacyl-tRNAs 到核糖体在蛋白质生合成期间。尽管证据显示出 isoform eEF1A1 oligomers 的存在,自我协会的实质的机制仍然保持不清楚。此处,我们发现人的 eEF1A1 能自发地形成 oligomers。明确地,半胱氨酸残余上的 mutagenesis 屏幕证明 Cys 234 为 eEF1A1 oligomerization 是必要的。另外,我们也发现氢过氧化物处理能在房间导致 eEF1A oligomers 的形成。由半胱氨酸代替, eEF1A2 isoform 在氧化环境下面在房间显示了能力到 oligomerize。在摘要,在这研究,我们描绘了 eEF1A1 oligomerization 并且证明特定的半胱氨酸残余为这项 oligomerization 活动被要求。 | Tao Liu Yu Yang Di Wang Yan Xiao Guangshi Du Lei Wu Muran Ding Ling Li Chuanfang Wu | 2015 | Acta Biochimica et Biophysica Sinica2015，47，12： | 1 |
| 7 | 17β-Estradiol treatment inhibits breast cell proliferation, migration and invasion by decreasing MALAT-1 RNA level显示文摘 | Ziyi Zhao Changjin Chen Yu Liu Chuanfang Wu | 2014 | Biochemical and Biophysical Research Communications2014，，： | 1 |
您所在的机构暂未开通此部分的功能，请联系管理员