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| 1 | Gold immunochromatographic sensor for the rapid detection of twenty-six sulfonamides in foods显示文摘一个金 immunochromatographic 传感器(GICS ) 在蜂蜜样品为 26 硫安类药剂的快速的察觉被开发。传感器基于能认出所有 26 硫安类药剂的组特定的 monoclonal 抗体(mAb ) 。三 haptens (1 与 thiazole 包围的 hapten, 2 与苯包围的 hapten,和 3 与直碳锁住的 hapten ) 被用于抗原准备。与 hybridoma 技术,组特定的 mAb 与 50% 最大的禁止的集中被屏蔽(对 sulfathizole (STZ ) 和从 0.08 ~ 90.18 ng/mL 的另外的 25 类似物的 IC 50) 。单音分散金 nanoparticles 与 mAb 被结合开发侧面的 immunochromatographic 长带。0.1 g/mL 的标记的抗体集中和在测试线的 0.2 g/mL 的涂层抗原集中为长带准备被选择。在优化条件,为 STZ 的集中的察觉( vLOD )的视觉限制, sulfamethoxazole , sulfamethizole ,磺胺嘧啶, sulfamerazine , sulfadimethoxine , sulfamonomethoxine , sulfameter , sulfamethoxypyridazine ,和 sulfachloropyridazine 下面是 5 , 0.25 , 0.25 , 10 , 5 , 10 , 25 , 2.5 , 5 , 0.25 ,和 10 g/kg 分别地。在蜂蜜样品的扫描仪分析为 26 硫安类药剂的察觉揭示了好性能。商业蜂蜜样品与传感器被测试,积极结果与高效的液体层析被证实。建议脱衣传感器在爱人样品为硫安类药剂污染物质的快速、可靠的决心提供一个方便方法。 | Yanni Chen Liqiang Liu Liguang Xu Shanshan Song Hua Kuang Gang Cui Chuanlai Xu | 2017 | Nano Research2017，10，8： | 11 |
| 2 | Gold nanoparticle-based paper sensor for ultrasensitive and multiple detection of 32 (fluoro)quinolones by one monoclonal antibody显示文摘为快速、同时的察觉(fluoro ) quinolones,认出 32 的广泛地特定的 monoclonal 抗体(mAb )(fluoro ) quinolone 抗菌素作为 hapten 用 norfloxacin 衍生物和 sarfloxacin 衍生物的混合物被准备。基于黄金, nanoparticles (AuNPs ) 当时是的 immunochromatographic 脱衣与山羊反老鼠抗体和抗原(联合到 ovalbumin 的 sarfloxacin ) 集合了,过去常分别地形成 C 线和 T 线。这抗原与竞争(fluoro ) 在与 mAbs 孵化的一件样品的 quinolones 用 AuNPs 标记。长带能检测 32 ( fluoro ) quinolones 包括 oxolinic 酸, nalidixic 酸, miloxacin , pipemidic 酸, piromidic 酸, rosoxacin , cinoxacin , norfloxacin , pefloxacin , lomfloxacin , enofloxacin , fleroxacin , ciprofloxacin , enrofloxacin , dafloxacin , orbifloxacin , sparfloxacin , gemifloxacin , besifloxacin , balofloxacin , gatifloxacin ,在在 10 mi 以内的牛奶的 moxifloxacin , nadifloxacin , ofloxacin , marbofloxacin , flumequine , pazufloxacin , prulifloxacin , sarafloxacin , difloxacin , trovafloxacin ,和 tosufloxacin 从 1 ~ 100 ng/mL 和察觉的限制的长带范围的截止价值是 0.1-10 ng/mL。长带不包括四圜素, sulfamethazine,氨比西林,抗生素的一种,黄麴毒素 B1,或 gentamicin 与抗菌素做跨 react。简言之,这 immunochromatographic 脱衣是为主要屏蔽的一个很有用的工具(fluoro ) 在牛奶的 quinolones。 | Juan Peng Liqiang Liu Liguang Xu Shanshan Song Hua Kuang Gang Cui Chuanlai Xu | 2017 | Nano Research2017，10，1： | 9 |
| 3 | Development of a faster determination of 10 anabolic steroids residues in animal muscle tissues by liquid chromatography tandem mass spectrometry 显示文摘 | XU Chuanlai CHU Xiaogang PENG Chifang | 2006 | J Pharm Biomed Anal2006，41，： | 1 |
| 4 | Simultaneous and sensitive determination of multiplex chemical residues based on multicolor quantum dot probes显示文摘 | Chifang Peng Zhuokun Li Yinyue Zhu Wei Chen Yuan Yuan Liqiang Liu Qiusheng Li Dinghua Xu Ruirui Qiao Libing Wang Shuifang Zhu Zhengyu Jin Chuanlai Xu | 2009 | Biosensors and Bioelectronics2009，，12： | 1 |
| 5 | Development of a faster determination of 10 anabolic steroids residues in animal muscle tissues by liquid chromatography tandem mass spectrometry显示文摘 | XU Chuanlai CHU Xiaogang PENG Chifang | 2006 | Journal of Pharmaceutical and Biomedical Analysis2006，41，2： | 1 |
| 6 | Multiple detection of 15 triazine herbicides by gold nanoparticle based-paper sensor显示文摘Triazine herbicides have been widely used in agriculture,but their residues can harm the environment and human health.To help monitor these,we have developed an effective immunochromatographic strip test that can simultaneously detect 15 different triazines in grain samples(including ametryn,cyprazine,atraton,prometon,prometryn,atrazine,propazine,terbuthylazine,simetryn,trietazine,secbumeton,simazine,desmetryn,terbumeton and simetone).Based on our optimization procedure,the visual limit of detection(vLOD)for these triazines was found to be 2–10 ng/mL in assay buffer,and 0.02–0.1 mg/kg in grain samples.Four different grain matrices including corn,brown rice,wheat,and sorghum were studied and the test results showed no significant differences between the 15 triazines analyzed using this method.This test is simple,convenient,rapid,and low-cost,and could be an effective tool for primary screening of triazine residues in grain samples. | Lingling Guo Xinxin Xu Jing Zhao Shudong Hu Liguang Xu Hua Kuang Chuanlai Xu | 2022 | Nano Research2022，15，6： | 1 |
| 7 | Development and optimization of an indirect enzyme-linked irnmunosorbent assay for thiarnpherdcol显示文摘 | HAO Kai GUO Shidong XU Chuanlai | 2006 | Analytical Letters2006，39，6： | 1 |
| 8 | Recent developments in analytical applications of quantum dots显示文摘 | Hua Kuang Yuan Zhao Wei Ma Liguang Xu Libing Wang Chuanlai Xu | 2011 | Trends in Analytical Chemistry2011，，10： | 1 |
| 9 | Determina- tion of Clenbuterol by CE with Chemluminescen detec- tion显示文摘 | Ji Xinghu He Zhike Xu Chuanlai | 2006 | Talanta2006，70，2： | 1 |
| 10 | Nucleic acids analysis显示文摘Nucleic acids are natural biopolymers of nucleotides that store, encode, transmit and express genetic information, which play central roles in diverse cellular events and diseases in living things. The analysis of nucleic acids and nucleic acids-based analysis have been widely applied in biological studies, clinical diagnosis, environmental analysis, food safety and forensic analysis.During the past decades, the field of nucleic acids analysis has been rapidly advancing with many technological breakthroughs.In this review, we focus on the methods developed for analyzing nucleic acids, nucleic acids-based analysis, device for nucleic acids analysis, and applications of nucleic acids analysis. The representative strategies for the development of new nucleic acids analysis in this field are summarized, and key advantages and possible limitations are discussed. Finally, a brief perspective on existing challenges and further research development is provided. | Yongxi Zhao Xiaolei Zuo Qian Li Feng Chen Yan-Ru Chen Jinqi Deng Da Han Changlong Hao Fujian Huang Yanyi Huang Guoliang Ke Hua Kuang Fan Li Jiang Li Min Li Na Li Zhenyu Lin Dingbin Liu Juewen Liu Libing Liu Xiaoguo Liu Chunhua Lu Fang Luo Xiuhai Mao Jiashu Sun Bo Tang Fei Wang Jianbin Wang Lihua Wang Shu Wang Lingling Wu Zai-Sheng Wu Fan Xia Chuanlai Xu Yang Yang Bi-Feng Yuan Quan Yuan Chao Zhang Zhi Zhu Chaoyong Yang Xiao-Bing Zhang Huanghao Yang Weihong Tan Chunhai Fan | 2021 | Science China Chemistry2021，64，2： | 1 |
| 11 | A highly sensitive enzyme-linked immunosorbent assay for copper(II) determination in drinking water显示文摘 | Changrui Xing Changlong Hao Liqiang Liu Chuanlai Xu Hua Kuang | 2014 | Food and Agricultural Immunology2014，，3： | 1 |
| 12 | Development of a monoclonal antibody-based sandwich ELISA for the detection of ovalbumin in foods显示文摘 | Juan Peng Xu Meng Xiaofang Deng Jianping Zhu Hua Kuang Chuanlai Xu | 2014 | Food and Agricultural Immunology2014，，1： | 1 |
| 13 | Development of a monoclonal antibody-based ELISA to detect Escherichia coli O157:H7显示文摘 | Min Feng Qianqian Yong Wenbing Wang Hua Kuang Libing Wang Chuanlai Xu | 2013 | Food and Agricultural Immunology2013，，4： | 1 |
| 14 | Monoclonal Antibody-Based Sandwich ELISA for the Detection of Staphylococcal Enterotoxin A显示文摘 | Kuang Hua Wang Wenbing Xu Liguang Ma Wei Liu Liqiang Wang Libing Xu Chuanlai | 2013 | International Journal of Environmental Research and Public Health2013，，4： | 1 |
| 15 | Development of ELISA for melamine detection in milk powder显示文摘 | Fengxia Sun Liqiang Liu Hua Kuang Chuanlai Xu | 2013 | Food and Agricultural Immunology2013，，1： | 1 |
| 16 | Determination of Clenbuterol by CE with Chemluminescen detection显示文摘 | Xinghu Ji Zhike He Chuanlai Xu | 2006 | Talanta2006，70，2： | 1 |
| 17 | Rapid and sensitive determination of difenoconazole in cucumber and pear samples using an immunochromatographic assay显示文摘Difenoconazole is triazole fungicide,widely used to prevent the growth and reproduction of fungi and to control fungal diseases in fruits and vegetables.In this work,we prepared a hapten of difenoconazole and produced a highly-sensitive anti difenoconazole-monoclonal antibody(mAb)with an IC50 of 0.64 ng/mL.Using the mAb,an immunochromatographic assay(ICA)was developed to analyze difenoconazole residues in pear and cucumber samples.The ICA exhibited LOD values of 2.60 and 2.30 ng/g for difenoconazole in pear and cucumber samples,respectively.The ICA showed average recoveries of difenoconazole ranging from 94.3%±3.6%–105.4%±1.6%,and CVs of 1.5–4.2%in cucumber and pear samples.When used to measure difenoconazole in samples,the ICA results were compatible with those detected by LC/MSMS.The results of this study support the idea that an ICA is a practical and effective tool for high-throughput and rapid analysis of difenoconazole residues in vegetables and fruits. | Jie Liu Xinxin Xu Aihong Wu Shanshan Song Liguang Xu Chuanlai Xu Liqiang Liu Hua Kuang | 2022 | Food Bioscience2022，47，3： | 0 |
| 18 | Metabolic profile of chiral cobalt oxide nanoparticles in vitro and in vivo显示文摘In this study,we investigated the in vivo behaviors of chiral cobalt oxide nanoparticles(Co_(3)O_(4) NPs)stabilized with D-or L-cysteine(denoted D-or L-NPs,respectively),as representative chiral metal oxide NPs.Chiral Co_(3)O_(4) NPs exerted no observable cytotoxicity within the tested dose range.Both D-NPs and L-NPs were internalized by dendritic cells through caveola-dependent endocytosis,and L-NPs entered the cells faster than D-NPs.Significantly,a metabolic analysis indicated that chiral L-NPs had the same effect on the level of bile acids in the liver as D-NPs,which is attributed to the almost equal amount of farnesoid X receptor(FXR)induced by the chiral NPs.This study suggests that low chiroptical activity nanomaterials would not affect the metabolism and kinetics under physiological conditions even if there is some difference in their transport. | Si Li Liwei Xu Meiru Lu Maozhong Sun Liguang Xu Changlong Hao Xiaoling Wu Chuanlai Xu Hua Kuang | 2021 | Nano Research2021，14，7： | 0 |
| 19 | Gold-based lateral-flow strip for the detection of penconazole in watermelon and cucumber samples显示文摘As a typical triazole fungicide,penconazole(PEN)is widely used in agriculture but has been proven to be toxic.In this study,we designed a new hapten to prepare a highly sensitive and specific anti-PEN monoclonal antibody(mAb)and established a gold nanoparticle-based lateral-flow immunoassay(LFIA)for the detection of PEN residues in watermelon and cucumber.The 50%inhibitory concentration(IC_(50))of the mAb was 0.42 ng/mL and the LFIA strip had a visual limit of detection(vLOD)of 2.5 ng/g and a cut-off value of 10 ng/g in watermelon and cucumbers.The calculated limit of detection(LOD)of the LFIA strip was 0.36 ng/g for watermelon and 0.29 ng/g for cucumber.The LFIA strip also gave a re-covery rate of 92.5%-109.0%for watermelon samples and 92.5%-106.7%for cucumber samples.These results using the LFIA strip are highly consistent with those seen using liquid chromatography-tandem mass spectrometry.Thus our developed LFIA strip represents a potentially reliable tool for the rapid on-site screening for PEN in watermelons and cucumbers. | Yang Liu Xinxin Xu Liqiang Liu Liguang Xu Hua Kuang Chuanlai Xu | 2022 | Food Quality and Safety2022，6，2： | 0 |
| 20 | Gold-nanoparticle-based multiplex immuno-strip biosensor for simultaneous determination of 83 antibiotics显示文摘Antibiotic residues,generated by the irrational use of drugs and environmental pollution,have always been a great challenge to aquaculture safety.Therefore,a quick,convenient,and performance-excellent way to detect antibiotic residues in aquaculture fish is urgently required.In this study,a multiplex immunochromatographic strip biosensor based on gold nanoparticles was developed for the simultaneous detection of five classes of antibiotic residues(24β-lactam antibiotics,26 sulfonamides,five tetracyclines,24 quinolones,and four amphenicols)in aquaculture fish within 10 min.The detection ranges of five representative antibiotics,penicillin G,sulfamethazine,tetracycline,enrofloxacin,and chloramphenicol,were 2.33–38.4,0.688–17.1,1.4–48.1,1.45–32.9,and 0.537–9.06μg/kg,respectively.The accuracy and stability of these measurements were demonstrated by analyzing spiked fish samples,with recovery rates of 87.5%–115.2%and a coefficient of variation<9.5%.The cross-reaction rates,based on the five representative antibiotics,were 3.77%–202%forβ-lactams,3.95%–137%for sulfonamides,9.19%–100%for tetracyclines,4.9%–145%for quinolones,and 3.2%–100%for amphenicols.The excellent testing performance of the biosensor strip to most of antibiotic residues in aquaculture fish ensures they meet the maximum residue limits required by countries or organizations.Therefore,this multiplex immunochromatographic strip biosensor is potentially applicable to the rapidly on-site determination of antibiotic residues in aquaculture fish. | Xianlu Lei Xinxin Xu Liqiang Liu Liguang Xu Li Wang Hua Kuang Chuanlai Xu | 2023 | Nano Research2023，16，1： | 0 |
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