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| 1 | TALEN or Cas9-Rapid,Efficient and Specific Choices for Genome Modifications显示文摘Precise modifications of complex genomes at the single nucleotide level have been one of the big goals for scientists working in basic and applied genetics,including biotechnology,drug development,gene therapy and synthetic biology.However,the relevant techniques for making these manipulations in model organisms and human cells have been lagging behind the rapid high throughput studies in the post-genomic era with a bottleneck of low efficiency,time consuming and laborious manipulation,and off-targeting problems.Recent discoveries of TALEs(transcription activator-like effectors) coding system and CRISPR(clusters of regularly interspaced short palindromic repeats) immune system in bacteria have enabled the development of customized TALENs(transcription activator-like effector nucleases) and CRISPR/Cas9 to rapidly edit genomic DNA in a variety of cell types,including human cells,and different model organisms at a very high efficiency and specificity.In this review,we first briefly summarize the development and applications of TALENs and CRISPR/Cas9-mediated genome editing technologies;compare the advantages and constraints of each method;particularly,discuss the expected applications of both techniques in the field of site-specific genome modification and stem cell based gene therapy;finally, propose the future directions and perspectives for readers to make the choices. | Chuanxian Wei Jiyong Liu Zhongsheng Yu Bo Zhang Guanjun Gao Renjie Jiao | 2013 | Journal of Genetics and Genomics2013，40，6： | 52 |
| 2 | Efficient and Specific Modifications of the Drosophila Genome by Means of an Easy TALEN Strategy显示文摘技术开发总是是在生物医学的研究驾驶突破的力量之一。自从托马斯·摩根的时间起,一步一步地, Drosophilists 为操作并且机能上地把开发了强大的基因工具为改进这些技术并且开发新技术的果蝇染色体,而是房间仍然大,今天特别作为生物学家开始系统地学习不同模型有机体的功能的 genomics ,包括人,以一种高产量的方式。这里,我们报导,第一次在果蝇,为借助于改进抄写在很高的效率修改果蝇染色体的一个快速、容易、高度特定的方法像使活跃之物的受动器核酸酶(TALEN ) 策略。我们利用了最近发达的单位集会策略装配设计修改黄基因的二特定的 TALEN (在性染色体上) 并且新奇正染色体的基因。TALEN 的 mRNAs 随后被注入果蝇胚胎。从 31.2% 注射 F0 肥沃的苍蝇,我们检测了包含黄基因的可继承的修正。从到可继承的修正的察觉的特定的 TALEN 的建设的全部过程能在一个月以内被完成。在果蝇的这个调停 TALEN 的染色体修正方法的潜在的应用程序被讨论。 | Jiyong Liu Changqing Li Zhongsheng Yu Peng Huang Honggang Wu Chuanxian Wei Nannan Zhu Yan Shen Yixu Chen Bo Zhang Wu-Min Deng Renjie Jiao | 2012 | Journal of Genetics and Genomics2012，39，5： | 43 |
| 3 | SCF^SImb E3 ligase-mediated degradation of Expanded is inhibited by the Hippo pathway in Drosophila显示文摘 | Hongtao Zhang Changqing Li Hanqing Chen Chuanxian Wei Fei Dai Honggang Wu Wen Dui Wu-Min Deng Renjie Jiao | 2015 | Cell Research2015，25，1： | 3 |
| 4 | HDAC6 regulates lipid droplet turnover in response to nutrient deprivation via p62-mediated selective autophagy显示文摘Autophagy has been evolved as one of the adaptive cellular processes in response to stresses such as nutrient deprivation. Various cellular cargos such as damaged organelles and protein aggregates can be selectively degraded through autophagy. Recently, the lipid storage organelle, lipid droplet(LD), has been reported to be the cargo of starvation-induced autophagy. However, it remains largely unknown how the autophagy machinery recognizes the LDs and whether it can selectively degrade LDs. In this study, we show that Drosophila histone deacetylase 6(dHDAC6), a key regulator of selective autophagy, is required for the LD turnover in the hepatocyte-like oenocytes in response to starvation. HDAC6 regulates LD turnover via p62/SQSTM1(sequestosome 1)-mediated aggresome formation, suggesting that the selective autophagy machinery is required for LD recognition and degradation. Furthermore, our results show that the loss of dHDAC6 causes steatosis in response to starvation. Our findings suggest that there is a potential link between selective autophagy and susceptible predisposition to lipid metabolism associated diseases in stress conditions. | Yan Yan Hao Wang Chuanxian Wei Yuanhang Xiang Xuehong Liang Chung-Weng Phang Renjie Jiao | 2019 | Journal of Genetics and Genomics2019，46，4： | 1 |
| 5 | Gain of function screen of PATs reveals an essential role of Hip14 in Drosophila host defense显示文摘The innate immune system is the first line of host defense against the invading pathogens in multicellular organisms,which is the key to eliminating pathogen infections,mitigating pathogen-induced inflammation,and activating adaptive immunity(Buchon et al.,2014;Liu and Cao 2016). | Zhu Deng Hui Chen Lingling Xiao Haolan Jin Qinhao Zhang Renjie Jiao Chuanxian Wei | 2023 | Journal of Genetics and Genomics2023，50，12： | 0 |
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