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| 1 | TALEN or Cas9-Rapid,Efficient and Specific Choices for Genome Modifications显示文摘Precise modifications of complex genomes at the single nucleotide level have been one of the big goals for scientists working in basic and applied genetics,including biotechnology,drug development,gene therapy and synthetic biology.However,the relevant techniques for making these manipulations in model organisms and human cells have been lagging behind the rapid high throughput studies in the post-genomic era with a bottleneck of low efficiency,time consuming and laborious manipulation,and off-targeting problems.Recent discoveries of TALEs(transcription activator-like effectors) coding system and CRISPR(clusters of regularly interspaced short palindromic repeats) immune system in bacteria have enabled the development of customized TALENs(transcription activator-like effector nucleases) and CRISPR/Cas9 to rapidly edit genomic DNA in a variety of cell types,including human cells,and different model organisms at a very high efficiency and specificity.In this review,we first briefly summarize the development and applications of TALENs and CRISPR/Cas9-mediated genome editing technologies;compare the advantages and constraints of each method;particularly,discuss the expected applications of both techniques in the field of site-specific genome modification and stem cell based gene therapy;finally, propose the future directions and perspectives for readers to make the choices. | Chuanxian Wei Jiyong Liu Zhongsheng Yu Bo Zhang Guanjun Gao Renjie Jiao | 2013 | Journal of Genetics and Genomics2013，40，6： | 52 |
| 2 | GL3.3, a Novel QTL Encoding a GSK3/SHAGGY- like Kinase, Epistatically Interacts with GS3 to Produce Extra-long Grains in Rice显示文摘 | Duo Xia Nao Zhou Rongjia Liu Wenhan Dan Pingbo Li Bian Wu Junxiao Chen Lingqiang Wang Guanjun Gao Qinglu Zhang Yuqing He | 2018 | Molecular Plant2018，11，5： | 41 |
| 3 | Genome-wide Association Analyses Reveal the Genetic Basis of Stigma Exsertion in Rice显示文摘耻辱 exsertion,交配系统的米饭的一个关键决定因素,极大地在米饭贡献杂种优势的申请。尽管与耻辱 exsertion 联系的一些量的特点 loci 是印射或克隆的罚款,内在的基因建筑学仍然保持不清楚。我们用在 Oryza sativa 的 533 多样的就职描绘的 650 万 SNP 在耻辱 exsertion 和相关花的特点上执行了染色体宽的协会研究。我们识别了显著地与耻辱 exsertion 和相关特点,其三与三主要谷物尺寸基因 GS3, GW5,和 GW2 co 局部性被联系的 23 genomic loci。进一步的分析显示这三基因由控制小穗状花小穗和耻辱的尺寸和形状影响了耻辱 exsertion。GS3 和 GW5 的联合大部分定义耻辱 exsertion 和相关特点的层次。这二基因的选择在 O 的 subpopulations 之中导致了花的特点的特定的分布。sativa。低耻辱 exsertion 联合 gw5GS3 在栽培米饭变化的一半存在;因此,介绍 GW5gs3 联合进男无菌的线具有为改进混合米饭的种子生产的高潜力。 | Hao Zhou Pingbo Li WeiboXie Saddam Hussain Yibo Li Duo Xia Hu Zhao Shengyuan Sun Junxiao Chen Hong Ye Jun Hou Da Zhao Guanjun Gao Qinglu Zhang Gongwei Wang Xingming Lian Jinghua Xiao Sibin Yu Xianghua Li Yuqing He | 2017 | Molecular Plant2017，10，4： | 15 |
| 4 | The origin of Wx^(la) provides new insights into the improvement of grain quality in rice显示文摘Appearance and taste are important factors in rice(Oryza sativa) grain quality. Here, we investigated the taste scores and related eating-quality traits of533 diverse cultivars to assess the relationships between—and genetic basis of—rice taste and eating-quality. A genome-wide association study highlighted the Wx gene as the major factor underlying variation in taste and eating quality. Notably, a novel waxy(Wx) allele, Wx^(la), which combined two mutations from Wx^(b) and Wx^(in), exhibited a unique phenotype. Reduced GBSSI activity conferred Wx^(la) rice with both a transparent appearance and good eating quality. Haplotype analysis revealed that Wx^(la) was derived from intragenic recombination. In fact,the recombination rate at the Wx locus was estimated to be 3.34 kb/c M, which was about 75-fold higher than the genome-wide mean, indicating that intragenic recombination is a major force driving diversity at the Wx locus. Based on our results, we propose a new network for Wx evolution, noting that new Wx alleles could easily be generated by crossing genotypes with different Wx alleles. This study thus provides insights into the evolution of the Wx locus and facilitates molecular breeding for quality in rice. | Hao Zhou Duo Xia Da Zhao Yanhua Li Pingbo Li Bian Wu Guanjun Gao Qinglu Zhang Gongwei Wang Jinghua Xiao Xianghua Li Sibin Yu Xingming Lian Yuqing He | 2021 | Journal of Integrative Plant Biology2021，63，5： | 8 |
| 5 | Kinematics and trajectory planning of a cucumber harvesting robot manipulator显示文摘In order to reduce cucumber harvesting cost and improve economic benefits,a cucumber harvesting robot was developed.The cucumber harvesting robot consists of a vehicle,a 4-DOF articulated manipulator,an end-effector,an upper monitor,a vision system and four DC servo drive systems.The Kinematics of the cucumber harvesting robot manipulator was constructed using D-H coordinate frame model.And the inverse kinematics which provides a foundation for trajectory planning has been solved with inverse transform technique.The cycloidal motion,which has properties of continuity and zero velocity and acceleration at the ports of the bounded interval,was adopted as a feasible approach to plan trajectory in joint space of the cucumber harvesting robot manipulator.Moreover,hardware and software based on CAN-bus communication between the upper monitor and the joint controllers have been designed.Experimental results show that the upper monitor communicates with the four joint controllers efficiently by CAN-bus,and the integrated errors of four joint angles do not exceed four degrees.Probable factors resulting in the errors were analyzed and the corresponding solutions for improving precision are proposed. | Zhang Libin Wang Yan Yang Qinghua Bao Guanjun Gao Feng Xun Yi | 2009 | International Journal of Agricultural and Biological Engineering2009，2，1： | 7 |
| 6 | Overview of research on agricultural robots in China显示文摘As the representation of new concept agricultural machinery,agricultural robots possess great advantages of improving agricultural productivity,enhancing production environment and solving the problem of labor shortage.Therefore,the strategy for application of agricultural robots and precision agriculture to improve the intelligence and information level of agriculture is the inevitable trend for China’s agriculture in the twentieth century.Based on the developmental status of agricultural robots in China,the agricultural robots are categorized and the performances,structures and characteristics of various agricultural robots such as vegetable grafting robots,transplanting robots,spraying robots,mowing robots,harvesting robots,grading and detecting robots are amply introduced.It can be seen that vegetable grafting robots,spraying robots,harvesting robots,grading and detecting robots have already been put into production while others are still at experimental stage.At present,there are several problems such as low popularization,great limitations,high cost and low intelligence,which greatly restrict the development of agricultural robots in China.Thus,open agricultural robot system with good expansibility,generality and flexibility should be developed and adopted to decrease its cost and shorten developing cycle.The mechanical structure of robots should also be designed as simply as possible.Finally,multi-robot system would become another important development direction of agricultural robots in the future. | Zhang Libin Yang Qinghua Bao Guanjun Wang Yan Qi Liyong Gao Feng Xu Fang | 2008 | International Journal of Agricultural and Biological Engineering2008，1，1： | 4 |
| 7 | Genetic architecture and key genes controlling the diversity of oil composition in rice grains显示文摘Rice grain oil is a valuable nutrient source.However,the genetic basis of oil biosynthesis in rice grains remains unclear.In this study,we performed a genome-wide association study on oil composition and oil concentration in a diverse panel of 533 cultivated rice accessions.High variation for 11 oil-related traits was observed,and the oil composition of rice grains showed differentiation among the subpopulations.We identified 46 loci that are significantly associated with grain oil concentration or composition,16 of which were detected in three recombinant inbred line populations.Twenty-six candidate genes encoding enzymes involved in oil metabolism were identified from these 46 loci,four of which(PAL6,LIN6,MYR2,and ARA6)were found to contribute to natural variation in oil composition and to show differentiation among the subpopulations.Interestingly,population genetic analyses revealed that specific haplotypes of PAL6 and LIN6 have been selected in japonica rice.Based on these results,we propose a possible oil biosynthetic pathway in rice grains.Collectively,our results provide new insights into the genetic basis of oil biosynthesis in rice grains and can facilitate marker-based breeding of rice varieties with enhanced oil and grain quality. | Hao Zhou Duo Xia Pingbo Li Yiting Ao Xiaodong Xu Shanshan Wan Yanhua Li Bian Wu Huan Shi Kaiyue Wang Guanjun Gao Qinglu Zhang Gongwei Wang Jinghua Xiao Xianghua Li Sibin Yu Xingming Lian Yuqing He | 2021 | Molecular Plant2021，14，3： | 4 |
| 8 | Effect and mechanism of YB-1 knockdown on glioma cell growth,migration,and apoptosis显示文摘Ybox binding protein 1(YB-1)is manifested as its involvement in cell proliferation and differ-entiation and malignant cell transformation.Overexpression of YB-1 is associated with glioma progression and patient survival.The aim of this study is to investigate the influence of YB-1 knockdown on glioma cell progression and reveal the mechanisms of YB-1 knockdown on glioma cell growth,migration,and apoptosis.It was found that the knockdown of YB-1 decreased the mRNA and protein levels of YB-1 in U251 glioma cells.The knockdown of YB-1 significantly inhibited cell proliferation,colony formation,and migration in vitro and tumor growth in vivo.Proteome and phosphoproteome data revealed that YB-1 is involved in glioma progression through regulating the expression and phosphorylation of major proteins involved in cell cycle,adhesion,and apoptosis.The main regulated proteins included CCNB1,CCNDBP1,CDK2,CDK3,ADGRG1,CDH-2,MMP14,AIFM1,HO-1,and BAX.Furthermore,it was also found that YB-1 knockdown is associated with the hypo-phosphorylation of ErbB,mTOR,HIF-1,cGMP-PKG,and insulin signaling pathways,and proteoglycans in cancer.Our findings indicated that YB-1 plays a key role in glioma progression in multiple ways.including regulating the expression and phosphorylation of major proteins associated with cell cycle,adhesion,and apoptosis. | Huilin Gong Shan Gao Chenghuan Yu Meihe Li Ping Liu Guanjun Zhang Jinning Song Jin Zheng | 2020 | Acta Biochimica et Biophysica Sinica2020，52，2： | 2 |
| 9 | Engineering Deinococcus radiodurans into biosensor to monitor radioactivity and genotoxicity in environment显示文摘Based on a genetically modified radioresistant bacteria Deinococcus radiodurans, we constructed a real time whole cell biosensor to monitor radioactivity and genotoxicity in highly radioactive environ-ment. The enhanced green fluorescence protein (eGFP) was fused to the promoter of the crucial DNA damage-inducible recA gene from D. radiodurans, and the consequent DNA fragment (PrecA-egfp) car-ried by plasmid was introduced into D. radiodurans R1 strain to obtain the biosensor strain DRG300. This engineered strain can express eGFP protein and generate fluorescence in induction of the recA gene promoter. Based on the correlation between fluorescence intensity and protein expression level in live D. radiodurans cells, we discovered that the fluorescence induction of strain DRG300 responds in a remarkable dose-dependent manner when treated with DNA damage sources such as gamma radiation and mitomycin C. It is encouraging to find the widely detective range and high sensitivity of this re-constructed strain comparing with other whole cell biosensors in former reports. These results suggest that the strain DRG300 is a potential whole cell biosensor to construct a detective system to monitor the biological hazards of radioactive and toxic pollutants in environment in real time. | GAO GuanJun FAN Lu LU HuiMing HUA YueJin | 2008 | Chinese Science Bulletin2008，53，11： | 2 |
| 10 | Ppd: a general switch responsible for extreme radioresistanee of Deinococcus radiodurans 显示文摘 | Hua Yuejin Narumi Issay Gao Guanjun | 2003 | Biochemical and Biophysical Research Communications2003，306，： | 1 |
| 11 | Function of a Low Molecular Weight Peptide from Trichoderma pseudokoningii S38 During Cellulose Biodegradation显示文摘 | Wei Wang Jie Liu Guanjun Chen Yingshu Zhang Peiji Gao | 2003 | Current Microbiology2003，，5： | 1 |
| 12 | Expression of Deinococcus radiodurans PprI enhances the radioresistance of Escherichia coli显示文摘 | Guanjun Gao Bing Tian Lili Liu Duohong Sheng Binghui Shen Yuejin Hua | 2003 | DNA Repair2003，，12： | 1 |
| 13 | Variation of airborne quartz in air of Beijing during the Asia-Pacific Economic Cooperation Economic Leaders' Meeting显示文摘Quartz particles are a toxic component of airborne paniculate matter(PM).Quartz concentrations were analyzed by X-ray diffraction in eighty-seven airborne PM samples collected from three locations in Beijing before,during,and after the Asia-Pacific Economic Cooperation(APEC) Leaders' Meeting in 2014.The results showed that the mean concentrations of quartz in PM samples from the two urban sites were considerably higher than those from the rural site.The quartz concentrations in samples collected after the APEC meeting,when the pollution restriction lever was lifted,were higher than those in the samples collected before or during the APEC meeting.The quartz concentrations ranged from 0.97 to 13.2 μg/m^3,which were among the highest values amid those reported from other countries.The highest quartz concentration exceeded the Californian Office of Environmental Health Hazard Assessment reference exposure level and was close to the occupational threshold limit values for occupational settings.Moreover,a correlation analysis showed that quartz concentrations were positively correlated with concentrations of pollution parameters PM_(10),PM_(2.5),SO_2 and NO_x,but were negatively correlated with O_3 concentration.The results suggest that the airborne quartz particles may potentially pose health risks to the general population of Beijing. | Gang Li Yingming Li Hongxing Zhang Honghua Li Guanjun Gao Qian Zhou Yuan Gao Wenjuan Li Huizhong Sun Xiaoke Wang Qinghua Zhang | 2016 | Journal of Environmental Sciences2016，28，1： | 1 |
| 14 | Deinococcus radio-durans PprI Switches on DNA Damage Response and Cellular Survi-val Networks after Radiation Damage 显示文摘 | Huiming Lu Guanjun Gao Guangzhi Xu et ai | 2009 | American Society for Bio-chemistry and Molecular Biology2009，8，： | 1 |
| 15 | Pyramiding and evaluation of three dominant brown planthopper resistance genes in the elite indica rice 9311 and its hybrids显示文摘 | Jie Hu Mingxing Cheng Guanjun Gao Qinglu Zhang Jinghua Xiao Yuqing He | 2013 | Pest Manag Sci2013，，7： | 1 |
| 16 | Expression of Deinococcus radiodurans PprI enhances the radioresistance of Escherichia coli显示文摘 | Guanjun Gao Bing Tian Lili Liu Duohong Sheng Binghui Shen Yuejin Hua | 2003 | DNA Repair2003，，12： | 1 |
| 17 | PprI: a general switch responsible for extreme radioresistance of Deinococcus radiodurans显示文摘 | Yuejin Hua Issay Narumi Guanjun Gao Bing Tian Katsuya Satoh Shigeru Kitayama Binghui Shen | 2003 | Biochemical and Biophysical Research Communications2003，，2： | 1 |
| 18 | Journal of Thermal Spray Technology显示文摘 | Gao Peihu Li Changjiu Yang Guanjun | 2008 | 2008, 17: 7422008，17，： | 1 |
| 19 | Thermofluidic investigations of oil natural transformer:Closed-loop modelling and experimental validation显示文摘Excessively high temperatures inside a power transformer can accelerate the ageing rate and even cause unplanned outages.Accurately estimating the hot-spot temperature(HST)of a power transformer is important for regulating the load and ensuring long-term operation.In this study,a closed-loop model of a 110-kV product transformer with an oil natural/air forced(ONAF)cooling mode is built to predict HST,in which the compression ratio of windings,equivalent heat source and radiator geometry are considered.According to the flow field of the winding,the‘dead oil zone’and reverse oil flow are observed in the horizontal oil channels.The accuracy of the proposed model is experimentally validated by temperature-rise tests.The results show that the absolute errors between the measured results and simulation are below 5°C,and the average error is~2°C.Furthermore,the arrangements of windings and radiators are studied to enhance the oil circulation.By adding 5 washers to the high-voltage winding,the average temperature can be decreased by 6°C compared with the case without a washer.Elevating the radiator can also mitigate the characteristic temperatures,and the optimal height difference between the radiator and winding centre is suggested to be 0.66 m. | Yuan Li Yiran Gao Chen Wang Chao Zhu Ying Li Yuankai Wang Guanjun Zhang | 2024 | High Voltage2024，9，1： | 0 |
| 20 | Microstructure and properties of Si_3N_4 ceramics obtained from addition of β-phase seeds and celsian显示文摘The silicon nitride based ceramics obtained by the addition of β-Si3N4 seeds was fabricated using celsian as the additive and by pressureless sintering. The microstructure of the material was observed and analyzed using XRD,SEM,and TEM and the mechanical response of this array microstructure was characterized for flexural strength and fracture behavior. The result shows that the growth of the larger aspect ratio grains initiate mainly from the seeds through the heterogeneous nucleation mechanism during the process of phase transformation,and the intergranular phase BaAl2Si2O8 services as good refractory materials. In contrast to conven-tional processing,a stable bimodal microstructure and complicated component with a lower cost can be achieved using the above-mentioned process theoretically and practically. | Jun Yang Jiqiang Gao Jianfeng Yang Guanjun Qiao Yonghong Luo | 2006 | Journal of University of Science and Technology Beijing2006，13，5： | 0 |
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