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| 1 | Effects of plant species coexistence on soil enzyme activities and soil microbial community structure under Cd and Pb combined pollution显示文摘The relationship between plant species coexistence and soil microbial communities under heavy metal pollution has attracted much attention in ecology.However,whether plant species coexistence could offset the impacts of heavy metal combined pollution on soil microbial community structure and soil enzymes activities is not well studied.The modified ecological dose model and PCR-RAPD method were used to assess the effects of two plant species coexistence on soil microbial community and enzymes activities subjected to Cd and Pb combined stress.The results indicated that monoculture and mixed culture would increased microbe populations under Cd and Pb combined stress,and the order of sensitivity of microbial community responding to heavy metal stress was:actinomycetes > bacteria > fungi.The respirations were significantly higher in planted soil than that in unplanted soil.The plant species coexistence could enhance soil enzyme activities under Cd and Pb combined.Furthermore,planted soil would be helpful to enhance soil genetic polymorphisms,but Cd and Pb pollution would cause a decrease on soil genetic polymorphisms.Mixed culture would increase the ecological dose 50%(ED50) values,and the ED50 values for soil enzyme activities decreased with increasing culture time.The dehydrogenase was most sensitive to metal addition and easily loses activity under low dose of heavy metal.However,it was difficult to fully inhibit the phoshpatase activity,and urease responded similarly with phosphatase. | Yang Gao Pei Zhou Liang Mao Yueer Zhi Chunhua Zhang Wanjun Shi | 2010 | Journal of Environmental Sciences2010，22，7： | 19 |
| 2 | Recent advances in cell sheet technology for bone and cartilage regeneration: from preparation to application显示文摘Bone defects caused by trauma,tumour resection,infection and congenital deformities,together with articular cartilage defects and cartilage–subchondral bone complex defects caused by trauma and degenerative diseases,remain great challenges for clinicians.Novel strategies utilising cell sheet technology to enhance bone and cartilage regeneration are being developed.The cell sheet technology has shown great clinical potential in regenerative medicine due to its effective preservation of cell–cell connections and extracellular matrix and its scaffold-free nature.This review will first introduce several widely used cell sheet preparation systems,including traditional approaches and recent improvements,as well as their advantages and shortcomings.Recent advances in utilising cell sheet technology to regenerate bone or cartilage defects and bone–cartilage complex defects will be reviewed.The key challenges and future research directions for the application of cell sheet technology in bone and cartilage regeneration will also be discussed. | Yuezhi Lu Wenjie Zhang Jie Wang Guangzheng Yang Shi Yin Tingting Tang Chunhua Yu Xinquan Jiang | 2019 | International Journal of Oral Science2019，11，2： | 14 |
| 3 | Evaluation of the Trend Uncertainty in Summer Ozone Valley over the Tibetan Plateau in Three Reanalysis Datasets显示文摘Trend uncertainty in the ozone valley over the Tibetan Plateau(OVTP)and the South Asian high(SAH)during1979–2009 in ERA-Interim(interim reanalysis data from the ECMWF),JRA-55(55-yr reanalysis data from the Japan Meteorological Agency),and NCEP-CFSR(Climate Forecast System Reanalysis)datasets was evaluated.The results showed that the NCEP-CFSR OVTP became strong in the summers of 1979–2009,whereas it became weak according to ERA-Interim and JRA-55.Satellite data merged with TOMS(Total Ozone Mapping Spectrometer)and OMI(Ozone Monitoring Instrument)agreed with the OVTP trend of NCEP-CFSR.The OVTP strengthening in NCEP-CFSR may have been caused by SAH intensification,a rising tropopause,and increasing ozone over non-TP(non-Tibetan Plateau)areas(27°–37°N,<75°E and>105°E).Analogously,the OVTP weakening in ERA-Interim and JRA-55 may have been affected by weakening SAH,descending tropopause,and decreasing non-TP ozone. | Dong GUO Yucheng SU Xiuji ZHOU Jianjun XU Chunhua SHI Yu LIU Weiliang LI Zhenkun LI | 2017 | Journal of Meteorological Research2017，31，2： | 13 |
| 4 | Tonifying Qi and activating blood circulation in terms of Traditional Chinese Medicine: their effects in patients with myocardial infarction显示文摘OBJECTIVE: To investigate the effects of tonifying Qi and activating blood circulation(SQABC), a method in Traditional Chinese Medicine(TCM), on end-point events in patients with myocardial infarction(MI) in this retrospective cohort study.METHODS: Clinical data were obtained from the medical records of patients with acute MI(AMI),both during hospitalization and follow-up, and included general demographic information(age, gender, and contact information), TCM regimens used,and end-point events.RESULTS: A total of 1596 patients with AMI were enrolled to this study, but data of only 1210 casesare accessible till follow-up. We classified the patients based on the exposure levels of SQABC.When comparing the results between all exposure and non-exposure groups, significant differences were identified, both during hospitalization and follow-ups. During hospitalization, cardiac death(4.40% vs 21.55%, P < 0.05) and cardiac shock(3.04% vs 11.62%, P < 0.05) were significantly lower in the exposure group than the non-exposure group. Similarly, during the follow-up, cardiac death(12.04% vs 20.49%, P < 0.05), acute heart failure(7.27% vs 11.81%, P < 0.05), composite endpoint of reinfarction and stroke(9.11% vs 15.28%,P < 0.05), and rehospitalization due to angina(25.49% vs 34.38%, P < 0.05) were significantly lower in the exposure group than the non-exposure group.CONCLUSION: Our findings suggest that SQABC can significantly benefits the subjects in the management of high-risk AMI in them. | Gao Wulin Dai Guohua Zhang Tong Bi Dongxue Liu Chunhua Shi Xiaojing Zhao Fang Zhao Chen | 2018 | Journal of Traditional Chinese Medicine2018，38，5： | 7 |
| 5 | Isolation of Growth Hormone-Releasing Hormone and Its Receptor Genes from Scatophagus argus and Their Expression Analyses显示文摘The teleost Scatophagus argus is a species whose females grows faster than males.Growth hormone(gh)mRNA abundance in females pituitary is higher than that in males;however the mechanism underlining such differential is still unknown.Growth hormone(GH)is tightly associated with GH-releasing hormone(Ghrh)in vertebrates.In this study,Ghrh gene(ghrh)and its receptor gene,ghrhr,were isolated from S.argus.Tissue expression analysis showed that ghrh and ghrhr were mainly expressed in hypothalamus while ghrhr was expressed in pituitary and gh was predominantly expressed in pituitary.Twenty cultured S.argus individuals were used to compare ghrh,ghrhr and gh mRNA abundances,120 g and 181 g average weight for male(n=11)and female(n=9),respectively.Real-time PCR indicated that the ghrh and ghrhr mRNA abundances in male hypothalamus were significantly higher than those in female hypothalamus while that of gh mRNA abundance was significantly higher in female pituitary than in male pituitary.The ghrh and ghrhr mRNA abundances were significantly up-regulated in female hypothalamus 3 h after injection of 0.1 mg kg^-1 body weight Ghrh while pituitary ghrhr and gh mRNA abundances were not affected.In female hypothalamus,ghrh and ghrhr m RNA abundances were not affected at 6 h post-injection of 4 mg kg^-1 body weight 17α-methyltes-tosterone(17α-MT)or 17β-Estradiol(E2).In female pituitary,ghrhr m RNA abundance was down-regulated by 17α-MT while that of gh m RNA abundance was up-regulated by E2.Our findings indicated that E2,rather than Ghrh,plays an important role in up-regulating the expression of gh in female S.argus,which should aid to understand the sexual dimorphism of teleost growth. | JIANG Dongneng SHI Hongjuan LIU Qianqing WANG Tuo HUANG Yuanqing HUANG Yang DENG Siping CHEN Huapu TIAN Changxu ZHU Chunhua LI Guangli | 2019 | Journal of Ocean University of China2019，18，6： | 6 |
| 6 | Antisymmetric transport of middle stratospheric methanewith respect to the equator显示文摘Halogen Occultation Experiment (HALOE)data are used to study the quasi-biennial variability ofCH4 mixing ratio in the middle stratosphere. The re-sults of EOF analysis indicate that quasi-biennialperiod is principal for the interannual variability ofmethane. Antisymmetry with respect to the equator issignificant for the methane QBO by explaining 59.3%variance, while the symmetric component only ex-plains about 30%. The antisymmetry is more signifi-cant in 10°-20° latitude than between 10oS and10oN. Analyses of the vertical motions show thatanomalies of annual cycle play an important role inthe antisymmetric distributions. Whereas, the zonalwind QBO is still the most important dynamical effectfor the interannual variability of CH4 between 10°S-10°N. | ZHENG Bin SHI Chunhua CHEN Yuejuan | 2006 | Chinese Science Bulletin2006，51，14： | 5 |
| 7 | Neuronal tolerance to hypoxia-ischemia through recombinant adeno-associated viral vectors expressing neuronal and inducible nitric oxide synthase An in vivo study显示文摘BACKGROUND: Studies have confirmed that neuronal nitric oxide synthase (nNOS) mediates neurotoxic effects during the early stages of hypoxia-ischemia,while inducible nitric oxide synthase (iNOS) mediates delayed neurotoxicity during advanced stages of hypoxia-ischemia. OBJECTIVE: This study was designed to observe neuronal apoptosis and the expressions of nNOS,iNOS,p38 mitogen-activated protein kinase (MAPK),and caspase-3 mRNA following transfection of recombinant adeno-associated viral vectors separately expressing nNOS and iNOS antisense (rAAV-AsnNOS and rAVV-AsiNOS,respectively) into rat brains subjected to cerebral ischemia; to analyze mechanisms underlying elevated neuronal tolerance to hypoxia-ischemia. DESIGN: A randomized controlled in vivo experiment. SETTING: Fujian Institute of Neurosurgery & Department of Neurosurgery,Union Hospital,Fujian Medical University. MATERIALS: Eighty healthy adult male Sprague Dawley rats of clean grade were provided by the Zhejiang Laboratory Animal Center,China. The protocol was performed in accordance with ethical guidelines for the use and care of animals. The following vectors,rAAV-AsnNOS,rAAV-AsiNOS,and rAAV expressing the β-galactosidase gene (rAAV-LacZ),were successfully constructed by Fujian Institute of Neurosurgery. Rabbit anti-mouse nitrotyrosine (NT) monoclonal antibody (Zhongshan Jinqiao Biotechnology Co.,Ltd.,Beijing,China) and reverse transcription-polymerase chain reaction (RT-PCR) kit (two-step method) (Promega Company,USA) were used in this study. METHODS: This study was performed at the Fujian Institute of Neurosurgery in December 2003. Sixty rats were randomly divided into 3 groups,with 20 rats in each group: rAAV-AsnNOS group,rAAV-AsiNOS group,and rAAV-LacZ group. The remaining 20 rats served as controls. Pre-treated viral vectors (rAAV-AsnNOS,rAAV-AsiNOS,and rAAV-LacZ,respectively; each 50 μL,virus titer of 2×109 viral particles/mL) were transfected into the cerebral cortex of the targeted. Phosphate buffer saline (50 μL) was perfused identically into the rat cerebral cortex of the control group. All rats were subjected to cerebral ischemia by occluding the right middle cerebral artery with suture. Five time points (0,1,6,24,72 hours of ischemia,4 rats for each time point) were allotted to each group. Ischemic brain tissue specimens were prepared for index measurement. MAIN OUTCOME MEASURES: The percentage of NT-positive cells and apoptotic cells in the rat ischemic brain tissue specimens were measured by flow cytometry. The expressions of nNOS,iNOS,p38MAPK,and caspase-3 mRNA were measured by RT-PCR. RESULTS: During the early stages of ischemia (at 1 and 6 hours) with rAAV-AsnNOS transfection,the percentage of NT-positive cells,apoptotic cells,and the expressions of nNOS,p38MAPK and caspase-3 mRNA in the brain nerve cells were remarkably reduced compared to the control,rAAV-LacZ,and rAAV-AsiNOS groups. During the advanced stages of ischemia (at 24 and 72 hours) with rAAV-AsiNOS transfection,the above-mentioned indices were significantly reduced compared to control,rAAV-LacZ,and rAAV-AsnNOS groups. CONCLUSION: Following rAAV transfection,neuronal cells resisted ischemic injury in the MCAO ischemic rat model. Neurons transfected with rAAV-AsnNOS inhibited expression of nNOS,p38MAPK,and caspase-3 during the early stages of ischemia,and those transfected by rAAV-AsiNOS inhibited above-mentioned index expression during the advanced stages of ischemia. These results clearly demonstrate that neuronal apoptosis can be inhibited through the use of nNOS antisense. | Chunmei Chen Weizhong Yang Chunhua Wang Songsheng Shi Jianping Chen Yong Huang Dongsheng Cai | 2008 | Neural Regeneration Research2008，3，2： | 3 |
| 8 | Mesenchymal Stem Cell-Mediated Immunosuppression Occurs via Concerted Action of Chemokines and Nitric Oxide显示文摘 | Guangwen Ren Liying Zhang Xin Zhao Guangwu Xu Yingyu Zhang Arthur I. Roberts Robert Chunhua Zhao Yufang Shi | 2008 | Cell Stem Cell2008，，2： | 3 |
| 9 | Evaluating the Brewer–Dobson circulation and its responses to ENSO,QBO,and the solar cycle in different reanalyses显示文摘This study compares the climatology and long-term trend of northern winter stratospheric residual mean meridional circulation(RMMC), as well as its responses to El Ni?o-Southern Oscillation(ENSO), stratospheric Quasi Biennial Oscillation(QBO), and solar cycle in ten reanalyses and a stratosphere-resolving model, CESM1-WACCM. The RMMC is a large-scale meridional circulation cell in the stratosphere, usually referred to as the estimate of the Brewer Dobson circulation(BDC). The distribution of the BDC is generally consistent among multiple reanalyses except that the NOAA twentieth century reanalysis(20RC) largely underestimates it. Most reanalyses(except ERA40 and ERA-Interim) show a strengthening trend for the BDC during 1979–2010. All reanalyses and CESM1-WACCM consistently reveal that the deep branch of the BDC is significantly enhanced in El Ni?o winters as more waves from the troposphere dissipate in the stratospheric polar vortex region. A secondary circulation cell is coupled to the temperature anomalies below the QBO easterly center at 50 hPa with tropical upwelling/cooling and midlatitude downwelling/warming, and similar secondary circulation cells also appear between 50–10 hPa and above 10 hPa to balance the temperature anomalies. The direct BDC response to QBO in the upper stratosphere creates a barrier near 30°N to prevent waves from propagating to midlatitudes, contributing to the weakening of the polar vortex. The shallow branch of the BDC in the lower stratosphere is intensified during solar minima, and the downwelling warms the Arctic lower stratosphere. The stratospheric responses to QBO and solar cycle in most reanalyses are generally consistent except in the two 20 CRs. | Jian Rao YueYue Yu Dong Guo ChunHua Shi Dan Chen DingZhu Hu | 2019 | Earth and Planetary Physics2019，3，2： | 3 |
| 10 | Systemic Infusion of FLK1+ Mesenchymal Stem Cells Ameliorate Carbon Tetrachloride-Induced Liver Fibrosis in Mice显示文摘 | Baijun Fang Mingxia Shi Lianming Liao Shaoguang Yang Yuhao Liu Robert Chunhua Zhao | 2004 | Transplantation2004，，1： | 2 |
| 11 | MicroRNA-575 targets BLID to promote growth and invasion of non-small cell lung cancer cells显示文摘 | Hongwei Wang Chunhua Yan Xiaodong Shi Jiaolin Zheng Lili Deng Lei Yang Fangfei Yu Yuandi Yang Yuxia Shao | 2015 | FEBS Letters2015，，： | 1 |
| 12 | Over-expression of nitric oxide and nitric oxide synthase in the rat brain following focal ischemia显示文摘BACKGROUND: In cerebral ischemia,over-expression of nitric oxide (NO) exerts neurotoxic effects. OBJECTIVE: This study was designed to measure NO and nitric oxide synthase (NOS) expression in rat brain tissue during cerebral ischemia,and to correlate expression levels of NO and NOS with ischemic time. DESIGN: A complete randomized grouping design,controlled animal experiment. SETTING: Fujian Institute of Neurosurgery & Department of Neurosurgery,Union Hospital Affiliated Fujian Medical University. MATERIALS: This study was performed at the Fujian Institute of Neurosurgery in December 2003. Eighty healthy adult male Sprague Dawley (SD) rats of clean grade were provided by the Zhejiang Laboratory Animal Center. The protocol was performed in accordance with ethical guidelines for the use and care of animals. Kits for measuring NO expression by method of nitrate reductase (Boster Company,Wuhan,China) and NOS activity (Jiancheng Bioengineering Co.,Ltd.,Nanjing,China),as well as a kit for reverse transcription-PCR (RT-PCR,Promega,USA) were used in the present study. METHODS: Sixty-eight rats underwent cerebral ischemia by occluding the middle cerebral artery by suture method. Forty-eight successfully lesioned rats were included in the study. Six rats were used for each length of ischemic event (30 minutes,1,3,6,12,24,and 72 hours,and 5 days of ischemia). Additionally,a normal control group (n = 6,normally raised) and a sham-operated group (n = 6,corresponding cerebral vessels exposed,without occlusion of middle cerebral artery) were included in the analysis. MAIN OUTCOME MEASURES: Neurological function deficits were scored according to methods of Longa,Bederson,and colleagues. NO levels and NOS activity in the brain tissue were measured via nitrate reductase and chemical colorimetry,respectively. The expression of nitrotyrosine (NT),an in vivo specific metabolite of NO,was quantitatively measured by flow cytometry. Expression of endothelial,neuronal,and inducible NOS mRNA (eNOS,nNOS,and iNOS mRNA,respectively) in the cerebral ischemic region were semi-quantitatively analyzed by RT-PCR. RESULTS: Forty-eight ischemic rats and six sham-operated rats were analyzed. Neurological functional deficits increased with ischemic times (r = 0.765,P < 0.05). There were no significant differences in NO levels,NOS activity,and the percentage of NT-positive cells between the sham-operated and the normal control groups (P > 0.05). NO levels,NOS activity,and the percentage of NT-positive cells in brain tissue positively correlated to ischemia times (r = 0.932,0.914,0.924,respectively,P < 0.05). The percentage of NT-positive cells began to noticeably increase within 0.5 hour of after ischemia (9.50 %). NOS activity began to increase within 0.5 hour of ischemia and reached its peak level 3 days after ischemia. RT-PCR semi-quantitative analysis demonstrated that in the early stages of ischemia (0.5-6 hours),the expression of both eNOS and nNOS mRNA increased with ischemic time (t = 28.482-100.459,P < 0.01),while iNOS mRNA levels were almost undetectable. In the middle and advanced stages of ischemia (6 hours-5 days),iNOS mRNA levels were significantly increased compared to the control group (t = 36.742-82.058,P < 0.01); however,eNOS and nNOS mRNA levels were markedly reduced. CONCLUSION: With the prolongation of ischemic time,NO levels increased in cerebral tissue due to activation of various NOS. These measurements correlated with an increase in NT-positive cells and behavioral deficits. In the early stages of ischemia,eNOS and nNOS activities were increased,while in the later stage of ischemia,iNOS activity was increased and eNOS and nNOS activities were reduced. | Weizhong Yang Chunmei Chen Chunhua Wang Songsheng Shi Junrong Lei Yongliang Zhang | 2008 | Neural Regeneration Research2008，3，2： | 1 |
| 13 | Enhanced porcine circovirus Cap protein production by Pichia pastoris with a fuzzy logic DO control based methanol/sorbitol co-feeding induction strategy显示文摘 | Jian Ding Chunling Zhang Minjie Gao Guoli Hou Kexue Liang Chunhua Li Jianping Ni Zhen Li Zhongping Shi | 2014 | Journal of Biotechnology2014，，： | 1 |
| 14 | HCl Quasi-biennial oscillation in the Ssratosphere and a comparison with ozone QBO 显示文摘 | Chen Yuejuan Shi Chunhua Zheng Bin | 2005 | Advance in Atmospheric Sciences2005，22，5： | 1 |
| 15 | Study on the trace species in the stratosphere and their impact on climate显示文摘 | Chen Yuejuan Zhou Renjun Shi Chunhua | 2006 | Advance in Atmospheric Sciences2006，23，6： | 1 |
| 16 | Integral Event-Triggered Attack-Resilient Control of Aircraft-on-Ground Synergistic Turning System With Uncertain Tire Cornering Stiffness显示文摘This article proposes an integral-based event-triggered attack-resilient control method for the aircraft-on-ground(AoG) synergistic turning system with uncertain tire cornering stiffness under stochastic deception attacks. First, a novel AoG synergistic turning model is established with synergistic reverse steering of the front and main wheels to decrease the steering angle of the AoG fuselage, thus reducing the steady-state error when it follows a path with some large curvature. Considering that the tire cornering stiffness of the front and main wheels vary during steering, a dynamical observer is designed to adaptively identify them and estimate the system state at the same time.Then, an integral-based event-triggered mechanism(I-ETM) is synthesized to reduce the transmission frequency at the observerto-controller end, where stochastic deception attacks may occur at any time with a stochastic probability. Moreover, an attackresilient controller is designed to guarantee that the closed-loop system is robust L2-stable under stochastic attacks and external disturbances. A co-design method is provided to get feasible solutions for the observer, controller, and I-ETM simultaneously. An optimization program is further presented to make a tradeoff between the robustness of the control scheme and the saving of communication resources. Finally, the low-and high-probability stochastic deception attacks are considered in the simulations. The results have illustrated that the AoG synergistic turning system with the proposed control method follows a path with some large curvature well under stochastic deception attacks. Furthermore,compared with the static event-triggered mechanisms, the proposed I-ETM has demonstrated its superiority in saving communication resources. | Chenglong Du Fanbiao Li Yang Shi Chunhua Yang Weihua Gui | 2023 | IEEE/CAA Journal of Automatica Sinica2023，10，5： | 1 |
| 17 | Real-time visual track- ing using compressive sensing 显示文摘 | Li Hanxi Shen Chunhua Shi Qinfeng | 2011 | IEEE Conference on Com- puter Vision Pattern Recognition2011，42，7： | 1 |
| 18 | Double cores of the Ozone Low in the vertical direction over the Asian continent in satellite data sets显示文摘Using four satellite data sets(TOMS/SBUV, OMI, MLS, and HALOE), we analyze the seasonal variations of the total column ozone(TCO) and its zonal deviation(TCO*), and reveal the vertical structure of the Ozone Low(OV) over the Asian continent. Our principal findings are:(1) The TCO over the Asian continent reaches its maximum in the spring and its minimum in the autumn. The Ozone Low exists from May to September.(2) The Ozone Low has two negative cores, located in the lower and the upper stratosphere. The lower core is near 30 hPa in the winter and 70 hPa in the other seasons. The upper core varies from 10 hPa to 1 hPa among the four seasons.(3)The position of the Ozone Low in the lower and the upper stratosphere over the Asian continent shows seasonal variability. | Zhou Tang Dong Guo YuCheng Su ChunHua Shi ChenXi Zhang Yu Liu XiangDong Zheng WenWen Xu JianJun Xu RenQiang Liu WeiLiang Li | 2019 | Earth and Planetary Physics2019，3，2： | 1 |
| 19 | New hope for cancer treatment:Exploring the distinction between normal adult stem cells and cancer stem cells显示文摘 | Robert Chunhua Zhao Ya-Shu Zhu Yufang Shi | 2008 | Pharmacol Therap2008，119，： | 1 |
| 20 | A long-term observation of olfactory ensheathing cells transplantation to repair white matter and functional recovery in a focal ischemia model in rat显示文摘 | Xianzhong Shi Yali Kang Qin Hu Chunhua Chen Lei Yang Ke Wang Lin Chen Hongyun Huang Changman Zhou | 2009 | Brain Research2009，，： | 1 |
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