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| 1 | A complete sequence and comparative analysis of a SARS-associated virus(Isolate BJ01)显示文摘The genome sequence of the Severe Acute Respiratory Syndrome (SARS)-associated virus provides essential information for the identification of pathogen(s), exploration of etiology and evolution, interpretation of transmission and pathogenesis, development of diagnostics, prevention by future vaccination, and treatment by developing new drugs. We report the complete genome sequence and comparative analysis of an isolate (BJ01) of the coronavirus that has been recognized as a pathogen for SARS. The genome is 29725 nt in size and has 11 ORFs (Open Reading Frames). It is composed of a stable region encoding an RNA-dependent RNA polymerase (composed of 2 ORFs) and a variable region representing 4 CDSs (coding sequences) for viral structural genes (the S, E, M, N proteins) and 5 PUPs (putative uncharacterized proteins). Its gene order is identical to that of other known coronaviruses. The sequence alignment with all known RNA viruses places this virus as a member in the family of Coronaviridae. Thirty putative substitutions have been identified by comparative analysis of the 5 SARS- associated virus genome sequences in GenBank. Fifteen of them lead to possible amino acid changes (non-synonymous mutations) in the proteins. Three amino acid changes, with predicted alteration of physical and chemical features, have been detected in the S protein that is postulated to beinvolved in the immunoreactions between the virus and its host. Two amino acid changes have been detected in the Mprotein, which could be related to viral envelope formation. Phylogenetic analysis suggests the possibility of non-human origin of the SARS-associated viruses but provides noevidence that they are man-made. Further efforts should focus on identifying the etiology of the SARS-associated virus and ruling out conclusively the existence of otherpossible SARS-related pathogen(s). | QIN E'de ZHU Qingyu YU Man FAN Baochang CHANG Guohui SI Bingyin YANG Bao PENG Wenming JIANG Tao LIU Bohua DENG Yongqiang LIU Hong ZHANG Yu WANG Cui LI Yuquan GAN Yonghua LI Xiaoyu L Fushuang TAN Gang CAO Wuchun, YANG Ruifu Institute of Microbiology and Epidemiology, Chinese Academy of Military Medical Sciences, Beijing 100071, China WANG Jian, LI Wei, XU Zuyuan, LI Yan, WU Qingfa, LIN Wei, CHEN Weijun, TANG Lin, DENG Yajun, HAN Yujun, LI Changfeng, LEI Meng, LI Guoqing, LI Wenjie, L Hong, SHI Jianping, TONG Zongzhong, ZHANG Feng, LI Songgang, LIU Bin, LIU Siqi, DONG Wei, WANG Jun, Gane K-S Wong, YU Jun & YANG Huanming* Beijing Genomics Institute, Chinese Academy of Sciences, Beijing 101300 National Center for Genome Information, Beijing 101300, China | 2003 | Chinese Science Bulletin2003，48，10： | 121 |
| 2 | D(β)-vertex-distinguishing total coloring of graphs显示文摘A new concept of the D(β)-vertex-distinguishing total coloring of graphs, i.e., the proper total coloring such that any two vertices whose distance is not larger than β have different color sets, where the color set of a vertex is the set composed of all colors of the vertex and the edges incident to it, is proposed in this paper. The D(2)-vertex-distinguishing total colorings of some special graphs are discussed, meanwhile, a conjecture and an open problem are presented. | ZHANG Zhongfu,LI Jingwen,CHEN Xiang’en,YAO Bing, WANG Wenjie & QIU Pengxiang Institute of Applied Mathematic, Lanzhou Jiaotong University, Lanzhou 730070, China College of Mathematics and Information Science, Northwest Normal University, Lanzhou 730070, China College of Information and Electrical Engineering, Lanzhou Jiaotong University, Lanzhou 730070, China | 2006 | Science China Mathematics2006，49，10： | 54 |
| 3 | A Survey on Terahertz Communications显示文摘With the exponential growth of the data traffic in wireless communication systems, terahertz(THz) frequency band is envisioned as a promising candidate to support ultra-broadband for future beyond fifth generation(5G), bridging the gap between millimeter wave(mmWave) and optical frequency ranges. The purpose of this paper is to provide a comprehensive literature review on the development towards THz communications and presents some key technologies faced in THz wireless communication systems. Firstly, despite the substantial hardware problems that have to be developed in terms of the THz solid state superheterodyne receiver, high speed THz modulators and THz antennas, the practical THz channel model and the efficient THz beamforming are also described to compensate for the severe path attenuation. Moreover, two different kinds of lab-level THz communication systems are introduced minutely, named a solid state THz communication system and a spatial direct modulation THz communication system, respectively. The solid state THz system converts intermediate frequency(IF) modulated signal to THz frequency while the direct modulation THz system allows the high power THz sources to input for approving the relatively long distance communications. Finally, we discuss several potential application scenarios as well as some vital technical challenges that will be encountered in the future THz communications. | Zhi Chen Xinying Ma Bo Zhang Yaxin Zhang Zhongqian Niu Ningyuan Kuang Wenjie Chen Lingxiang Li Shaoqian Li | 2019 | China Communications2019，16，2： | 43 |
| 4 | Analyses on the climate change responses over China under SRES B2 scenario using PRECIS显示文摘The PRECIS,a regional climate model system developed at the UK Met Office Hadley Cen- tre for Climate Prediction and Research,which is nested in one-way mode within the HadAM3P,a higher-resolution version of the atmospheric com- ponent of the Hadley Centre climate model HadCM3,is employed to simulate the baseline (1961—1990) climate for evaluation of model’s capacity of simu- lating present climate and analyze the future climate change responses in the time-slice of 2071—2100 (2080s) under SRES B2 scenario over China relative to baseline average. It is indicated from the com- parison of the simulated baseline climate with in situ observation that PRECIS can simulate the local dis- tribution characteristics of surface air temperature over China quite well; generally speaking,the simu- lation for precipitation in the north of China and in winter is better than in the south of China and in summer,respectively; the simulation of precipitation in summer is sensitive to topography,and the simu- lated precipitation values are lower than observations over southeast coastal areas. It is shown from the analyses on the simulated climate change responses in 2080s under SRES B2 scenario relative to base- line that there would be an obvious surface air tem- perature increase in the north of China relative to that in the south of China,and especially in Northwest China and Northeast China,the amplitude ofsummer mean surface air temperature increments could reach 5℃; there would be an overall increase of the simulated precipitation in 2080s under SRES B2 scenario over most areas of China,while there would be significant precipitation decreases in South China in winter; there would be obvious precipitation decreases in Northeast China and North China in summer with high surface air temperature increase. However,it presents an obvious precipitation in- crease over the middle and lower reaches of the Yangtze River in summer. | XU Yinlong ZHANG Yong LIN Erda LIN Wantao DONG Wenjie Richard Jones David Hassell Simon Wilson | 2006 | Chinese Science Bulletin2006，51，18： | 39 |
| 5 | Identification of gamyb-4 and Analysis of the Regulatory Role of GAMYB in Rice Anther Development显示文摘In higher plants,male reproductive development is a complex biological process that includes cell division and differentiation,cell to cell communication etc.,while the mechanism underlying plant male reproductive development remains less understood.GAMYB encodes a gibberellins acid(GA) inducible transcription factor that is required for the early anther development in rice(Oryza sativa L.).Here,we report the isolation and characterization of a new allele gamyb-4 with a C base deletion in the second exon(+2308),causing a frame shift and premature translational termination.Histological analysis showed that gamyb-4 developed abnormal enlarged tapetum and could not undergo normal meiosis.To understand the regulatory role of GAMYB,we carried out quantitative reverse transcription-polymerase chain reaction analysis and comparison of microarray data.These results revealed that the expression of TDR(TAPETUM DEGENERATION RETARDATION),a tapetal cell death regulator,was downregulated in gamyb-4 and udt1(undeveloped tapetum1).While the GAMYB expression was not obviously changed in tdr and udt1-1,and no apparent expression fold change of UDT1 in tdr and gamyb-4,suggesting that TDR may act downstream of GAMYB and UDT1,and GAMYB and UDT1 work in parallel to regulate rice early anther development.This work is helpful in understanding the regulatory network in rice anther development. | Zhenhua Liu Wenjie Bao Wanqi Liang Jingyuan Yin Dabing Zhang | 2010 | Journal of Integrative Plant Biology2010，52，7： | 35 |
| 6 | Reconstruction of Soil Particle Composition During Freeze-Thaw Cycling: A Review显示文摘在几十年进行的研究证明了结冰融化周期在土壤是精力输入和输出的进程,它帮助驾驶土壤结构的形成,通过由结晶化和由热坡度的水和盐的运动的水扩大。然而,大多数这些研究用俄语或汉语被出版并且对国际研究人员不太可存取。这评论在在土壤结构上结冰并且融化的效果上召集了大量研究。下列调查结果被总结:我) 在结冰融化周期以后的土壤结构更加变化,变化由于粗糙的矿物质粒子和土壤的聚集罚款的土壤的机械破碎粒子;ii ) 土壤的粒子尺寸变得同类,当结冰融化周期的数字增加,在土壤结构的变化变弱;iii ) 在土壤的结冰过程,在土壤粒子结合的变化的一个重要原则表现为:冷凝作用 →
聚集 →
结晶化;iv ) 结冰融化骑车的过程由在土壤以内改变矿物质粒子和结构的 granulometric 作文在土壤结构上有强壮的效果。骑车的过程加强粒子结合的结冰融化,在土壤的总数稳定性引起全面增加,从破坏显示出一个过程到重建。 | ZHANG Ze MA Wei FENG Wenjie XIAO Donghui HOU Xin | 2016 | Pedosphere2016，26，2： | 37 |
| 7 | The structural and accessory proteins M,ORF 4a,ORF 4b,and ORF 5 of Middle East respiratory syndrome coronavirus(MERS-CoV)are potent interferon antagonists显示文摘The newly emerged Middle East respiratory syndrome coronavirus(MERS-CoV)is a highly pathogenic respira-tory virus with pathogenic mechanisms that may be driven by innate immune pathways.The goal of this study is to characterize the expression of the structural(S,E,M,N)and accessory(ORF 3,ORF 4a,ORF 4b,ORF 5)proteins of MERS-CoV and to determine whether any of these pro-teins acts as an interferon antagonist.Individual structural and accessory protein-coding plasmids with an N-terminal HA tag were constructed and transiently transfected into cells,and their native expression and subcellular localiza-tion were assessed using Wes tern blotting and indirect immunofl uorescence.While ORF 4b demonstrated majorly nuclear localization,all of the other proteins demonstrated cytoplasmic localization.In addition,for the fi rst time,our experiments revealed that the M,ORF 4a,ORF 4b,and ORF 5 proteins are potent interferon antagonists.Further exami-nation revealed that the ORF 4a protein of MERS-CoV has the most potential to counteract the antiviral effects of IFN via the inhibition of both the interferon production(IFN-βpromoter activity,IRF-3/7 and NF-κB activation)and ISRE promoter element signaling pathways.Together,our re-sults provide new insights into the function and pathogenic role of the structural and accessory proteins of MERS-CoV. | Yang Yang Ling Zhang Heyuan Geng Yao Deng Baoying Huang Yin Guo Zhengdong Zhao Wenjie Tan | 2013 | Protein & Cell2013，4，12： | 25 |
| 8 | Interleukin-33 drives hepatic fibrosis through activation of hepatic stellate cells显示文摘Liver fibrosis is a consequence of chronic liver disease,causing morbidity and mortality.Interleukin-33(IL-33)is a critical mediator of inflammation,which may be involved in the development of liver fibrosis.Here,we investigated the role of IL-33 in human patients and experimental bile-duct ligation(BDL)-induced fibrosis in mice.We report increased hepatic IL-33 expression in the murine BDL model of fibrosis and in surgical samples obtained from patients with liver fibrosis.Liver injury,inflammatory cell infiltration and fibrosis were reduced in the absence of the IL-33/ST2 receptor,and the activation of hepatic stellate cells(HSCs)was decreased in ST2-deficient mice.Recombinant IL-33 activated HSCs isolated from C57BL/6 mice,leading to the expression of IL-6,TGF-β,α-SMA and collagen,which was abrogated in the absence of ST2 or by pharmacological inhibition of MAPK signaling.Finally,administration of recombinant IL-33 significantly increased hepatic inflammation in sham-operated BL6 mice but did not enhance BDL-induced hepatic inflammation and fibrosis.In conclusion,BDL-induced liver inflammation and fibrosis are dependent on ST2 signaling in HSCs,and therefore,the IL-33/ST2 pathway may be a potential therapeutic target in human patients with chronic hepatitis and liver fibrosis. | Zhongming Tan Qianghui Liu Runqiu Jiang Long Lv Siamak S Shoto Isabelle Maillet Valerie Quesniaux Junwei Tang Wenjie Zhang Beicheng Sun Bernhard Ryffel | 2018 | Cellular & Molecular Immunology2018，15，4： | 24 |
| 9 | Vegetation effects on mean daily maximum and minimum surface air temperatures over China显示文摘Changes in the daily maximum (Tmax) and minimum (Tmin) surface air temperatures and the associated temperature extremes have severe consequences on human society and the natural environment. In this study, we assess vegetation effects on mean Tmax and Tmin over China by computing a vegetation feedback parameter using the satellite-sensed Normalized Difference Vegetation Index (NDVI) and observed temperatures for the period 1982–2002. In all seasons, vegetation exerts a much stronger forcing on Tmax than on Tmin, and thus has a substantial effect on the diurnal temperature range (DTR) over China. Significant positive feedbacks on Tmax and the DTR occupy many areas of China with the feedback parameters exceeding 1°C (0.1 NDVI)–1, while significant negative effects only appear over the summertime climatic and ecological transition zone of northern China and some other isolated areas. Also, the vegetation feedbacks are found to vary with season. In areas where significant feedbacks occur, vegetation contributes to typically 10%–30% of the total variances in Tmax, Tmin, and the DTR. These findings suggest that vegetation memory offers the potential for improving monthly-to-seasonal forecasting of Tmax and Tmin, and the associated temperature extremes over China. Meanwhile, the limitations and uncertainties of the study should be recognized. | WU LingYun ZHANG JingYong DONG WenJie | 2011 | Chinese Science Bulletin2011，56，9： | 22 |
| 10 | Trends in particulate matter and its chemical compositions in China from 2013–2017显示文摘Accurate determination of the atmospheric particulate matter mass concentration and chemical composition is helpful in exploring the causes and sources of atmospheric enthalpy pollution and in evaluating the rationality of environmental air quality control strategies.Based on the sampling and chemical composition data of PM2.5 in different key regions of China in the CARE-China observation network,this research analyzes the environmental air quality data released by the China National Environmental Monitoring Centre during the studied period to determine the changes in the particulate matter mass concentration in key regions and the evolution of the corresponding chemical compositions during the implementation of the Action Plan for Prevention and Control of Air Pollution from 2013-2017.The results show the following.(1)The particulate matter mass concentration in China showed a significant downward trend;however,the PM2.5 annual mass concentration in 64%of cities exceeds the New Chinese Ambient Air Quality Standard(CAAQS)GradeⅡ(GB3095-2012).The region to the east of the Taihang Mountains,the Fenhe and Weihe River Plain and the Urumqi-Changji regions in Xinjiang,all have PM2.5 concentration loading that is still high,and heavy haze pollution occurred frequently in the autumn and winter.(2)During the heavy pollution in the autumn and winter,the concentrations of sulfate and organic components decreased significantly.The mean SO42-concentration in PM2.5 decreased by 76%,12%,81%and 38%in Beijing-Tianjin-Hebei(BTH),the Pearl River Delta(PRD),the Sichuan-Chongqing region(SC)and the Fenhe and Weihe River Plain,respectively.The mean organic matter(OM)concentration decreased by 70%,44%,48%and 31%,respectively,and the mean concentration of NH4+decreased by 68%,1.6%,38%and 25%,respectively.The mean elemental carbon(EC)concentration decreased by 84%and 20%in BTH and SC,respectively,and it increased by 61%and 11%in the PRD and Fenhe and Weihe River Plain,respectively.The mean concentration of mineral and unresolved chemical components(MI)dropped by 70%,24%and 13%in BTH,the PRD and the Fenhe and Weihe River Plain,respectively.The change in the PM2.5 chemical composition is consistent with the decrease of the PM2.5mass concentration.(3)In 2015,the mean OM concentration contributions to fine particles and coarse particles were 13-46%and 46-57%,respectively,and the mean MI concentration contributions to fine particles and coarse and particles were 31-60%and 39-73%,respectively;these values are lower than the 2013 values from the key regions,which is the most important factor behind the decrease of the particulate matter mass concentration.From 2013 to 2015,among the chemical components of different particle size fractions,the peak value of the coarse particle size fraction decreased significantly,and the fine particle size fractions of SO42-,NO3-,and NH4+decreased with the decrease of the particulate matter mass concentration in different particle size fractions.The fine-particle size peaks of SO42-,NO3-and NH4+shifted from 0.65-1.1μm to the finer size range of0.43-0.65μm during the same time frame. | Yuesi WANG Wenjie LI Wenkang GAO Zirui LIU Shili TIAN Rongrong SHEN Dongsheng JI Shuai WANG Lili WANG Guiqian TANG Tao SONG Mengtian CHENG Gehui WANG Zhengyu GONG Jiming HAO Yuanhang ZHANG | 2019 | Science China Earth Sciences2019，62，12： | 23 |
| 11 | Characterization of drug responses of mini patient-derived xenografts in mice for predicting cancer patient clinical therapeutic response显示文摘Background:Patient-derived organoids and xenografts(PDXs)have emerged as powerful models in functional diag-nostics with high predictive power for anticancer drug response.However,limitations such as engraftment failure and time-consuming for establishing and expanding PDX models followed by testing drug efficacy,and inability to subject to systemic drug administration for ex vivo organoid culture hinder realistic and fast decision-making in selecting the right therapeutics in the clinic.The present study aimed to develop an advanced PDX model,namely MiniPDX,for rapidly testing drug efficacy to strengthen its value in personalized cancer treatment.Methods:We developed a rapid in vivo drug sensitivity assay,OncoVee®MiniPDX,for screening clinically relevant regimens for cancer.In this model,patient-derived tumor cells were arrayed within hollow fiber capsules,implanted subcutaneously into mice and cultured for 7 days.The cellular activity morphology and pharmacokinetics were systematically evaluated.MiniPDX performance(sensitivity,specificity,positive and negative predictive values)was examined using PDX as the reference.Drug responses were examined by tumor cell growth inhibition rate and tumor growth inhibition rate in PDX models and MiniPDX assays respectively.The results from MiniPDX were also used to evaluate its predictive power for clinical outcomes.Results:Morphological and histopathological features of tumor cells within the MiniPDX capsules matched those both in PDX models and in original tumors.Drug responses in the PDX tumor graft assays correlated well with those in the corresponding MiniPDX assays using 26 PDX models generated from patients,including 14 gastric cancer,10 lung cancer and 2 pancreatic cancer.The positive predictive value of MiniPDX was 92%,and the negative predictive value was 81%with a sensitivity of 80%and a specificity of 93%.Through expanding to clinical tumor samples,Min-iPDX assay showed potential of wide clinical application.Conclusions:Fast in vivo MiniPDX assay based on capsule implantation was developed-to assess drug responses of both PDX tumor grafts and clinical cancer specimens.The high correlation between drug responses of paired MiniPDX and PDX tumor graft assay,as well as translational data suggest that MiniPDX assay is an advanced tool for personalized cancer treatment. | Feifei Zhang Wenjie Wang Yuan Long Hui Liu Jijun Cheng Lin Guo Rongyu Li Chao Meng Shan Yu Qingchuan Zhao Shun Lu Lili Wang Haitao Wang Danyi Wen | 2018 | Cancer Communications2018，38，1： | 20 |
| 12 | Characteristics of atmospheric single particles during haze periods in a typical urban area of Beijing:A case study in October,2014显示文摘To investigate the composition and possible sources of particles, especially during heavy haze pollution, a single particle aerosol mass spectrometer(SPAMS) was deployed to measure the changes of single particle species and sizes during October of 2014, in Beijing. A total of 2,871,431 particles with both positive and negative spectra were collected and characterized in combination with the adaptive resonance theory neural network algorithm(ART-2a). Eight types of particles were classified: dust particles(dust, 8.1%), elemental carbon(EC, 29.0%), organic carbon(OC, 18.0%), EC and OC combined particles(ECOC, 9.5%),Na-K containing particles(Na K, 7.9%), K-containing particles(K, 21.8%), organic nitrogen and potassium containing particles(KCN, 2.3%), and metal-containing particles(metal,3.6%). Three haze pollution events(P1, P2, P3) and one clean period(clean) were analyzed,based on the mass and number concentration of PM_(2.5)and the back trajectory results from the hybrid single particle Lagrangian integrated trajectory model(Hysplit-4 model). Results showed that EC, OC and K were the major components of single particles during the three haze pollution periods, which showed clearly increased ratios compared with those in the clean period. Results from the mixing state of secondary species of different types of particles showed that sulfate and nitrate were more readily mixed with carbon-containing particles during haze pollution episodes than in clean periods. | Lang Liu Yanli Wang Shiyong Du Wenjie Zhang Lujian Hou Sverre Vedal Bin Han Wen Yang Mindong Chen Zhipeng Bai | 2016 | Journal of Environmental Sciences2016，28，2： | 19 |
| 13 | Whole-genome sequencing of 508 patients identifies key molecular features associated with poor prognosis in esophageal squamous cell carcinoma显示文摘Esophageal squamous cell carcinoma(ESCC)is a poor-prognosis cancer type with limited understanding of its molecular etiology.Using 508 ESCC genomes,we identified five novel significantly mutated genes and uncovered mutational signature clusters associated with metastasis and patients’outcomes.Several functional assays implicated that NFE2L2 may act as a tumor suppressor in ESCC and that mutations in NFE2L2 probably impaired its tumor-suppressive function,or even conferred oncogenic activities.Additionally,we found that the NFE2L2 mutations were significantly associated with worse prognosis of ESCC.We also identified potential noncoding driver mutations including hotspot mutations in the promoter region of SLC35E2 that were correlated with worse survival.Approximately 5.9%and 15.2%of patients had high tumor mutation burden or actionable mutations,respectively,and may benefit from immunotherapy or targeted therapies.We found clinically relevant coding and noncoding genomic alterations and revealed three major subtypes that robustly predicted patients’outcomes.Collectively,we report the largest dataset of genomic profiling of ESCC useful for developing ESCC-specific biomarkers for diagnosis and treatment. | Yongping Cui Hongyan Chen Ruibin Xi Heyang Cui Yahui Zhao Enwei Xu Ting Yan Xiaomei Lu Furong Huang Pengzhou Kong Yang Li Xiaolin Zhu Jiawei Wang Wenjie Zhu Jie Wang Yanchun Ma Yong Zhou Shiping Guo Ling Zhang Yiqian Liu Bin Wang Yanfeng Xi Ruifang Sun Xiao Yu Yuanfang Zhai Fang Wang Jian Yang Bin Yang Caixia Cheng Jing Liu Bin Song Hongyi Li Yi Wang Yingchun Zhang Xiaolong Cheng Qimin Zhan Yanhong Li Zhihua Liu-Show | 2020 | Cell Research2020，30，10： | 19 |
| 14 | Investigating the dynamics of wetland landscape pattern in Beijing from 1984 to 2008显示文摘北京沼泽地的风景模式由于自然、人工的元素经历了一个重要变化。由遥感和 GIS 技术支持了,用在北京的从 1984~2008 的多时间的 TM 图象,这份报纸包括补丁区域通过选择典型索引分析了沼泽地风景模式的动态特征,补丁一般水准区域,分数维的尺寸索引,差异,优势,传染索引和每种沼泽地类型的空间矩心是计算的。最后,纸探索了进化模式和沼泽地风景模式的开车因素。结果如下被获得:全部的沼泽地区域在时期期间增加了 19841996,然后从 1996 ~ 2004 衰退。在 1994 的沼泽地区域说明了仅仅那中的 47.37% 个在 2004。人工的沼泽地区域的比例比自然沼泽地的大。水库沼泽地的比例是 33.50% ~ 53.73% 并且有最大的平均区域。有最少的平均区域的池溏和稻地沼泽地类型说明了 16.46% ~ 45.09% 全部的沼泽地区域。自然的河沼泽地的驱动力主要是自然元素;它的分数维的尺寸索引比其它大。沼泽地风景的香农差异索引在 2004 在 1992 ~ 1.34 从 1.11 增加了,显示每种沼泽地类型的比例之间的差别减少了,每种沼泽地类型的区域是均匀地分布式的。传染索引从 65.59 ~ 58.41 下沉了,显示连接降级了。Miyun 水库有最大的区域,它的区域变化在矩心的地点上有大影响。沼泽地资源从自然、人工的因素的联合效果逐渐地堕落了。在时期期间 20062008,增加的降水和干旱条件被减轻。政府实现了积极政策的系列节省水资源,并且沼泽地区域增加了。 | ZHANG Yiran GONG Zhaoning GONG Huili ZHAO Wenji | 2011 | Journal of Geographical Sciences2011，21，5： | 16 |
| 15 | COVID-19 Outbreak Caused by Contaminated Packaging of Imported Cold-Chain Products—Liaoning Province,China,July 2020显示文摘Summary What is known about this topic?Few major outbreaks of coronavirus disease 2019(COVID-19)have occurred in China after major nonpharmaceutical interventions and vaccines have been deployed and implemented.However,sporadic outbreaks that had high possibility to be linked to cold chain products were reported in several cities of China..What is added by this report?In July 2020,a COVID-19 outbreak occurred in Dalian,China.The investigations of this outbreak strongly suggested that the infection source was from COVID-19 virus-contaminated packaging of frozen seafood during inbound unloading personnel contact.What are the implications for public health practice?Virus contaminated paper surfaces could maintain infectivity for at least 17–24 days at-25℃.Exposure to COVID-19 virus-contaminated surfaces is a potential route for introducing the virus to a susceptible population.Countries with no domestic transmission of COVID-19 should consider introducing prevention strategies for both inbound travellers and imported goods.Several measures to prevent the introduction of the virus via cold-chain goods can be implemented. | Huilai Ma1 Jianqun Zhang Ji Wang Ying Qin Cao Chen Yang Song Liang Wang Jun Meng Lingling Mao Fengqin Li Ning Li Jian Cai Yong Zhang Dayan Wang Yunting Xia Hong Wang Shaofeng Jiang Xiang Zhao Peihua Niu Wenjie Tan Tao Ma Yecheng Yao Naiying Mao Zhen Zhu Tianjiao Ji Qian Yang Baoying Huang Li Zhao Jianxing Yu Li Bai Shuangli Zhu Dongyan Wang Yan Zhang Yingwei Sun Mingchun Luan Yanhai Wang Haibo Sun Shihong Yang Zhijian Bo Xiang Ren Zhongjie Li George Fu Gao Wei Yao Wenqing Yao Zijian Feng Wenbo Xu | 2021 | China CDC weekly2021，3，21： | 16 |
| 16 | High-precision timing of the Quaternary standard samples with thermal ionization mass spectrometry (TIMS) U-series method显示文摘Two national stalagmite standard (GBW04412, GBW04413) and one international coral standard (RKM4) have been determined by using thermal ionization mass spectrometry (TIMS) method. The values of ( 234 U/ 238 U) act , ( 230 Th/ 234 U) act and age are all consistent in error range with the standard values obtained by using α spectrometry. | Peng Zicheng Wang Zhaorong Sun Weidong Ma Zhibang Xia Ming Zhang Chenhui Chen Wenji Zhang Zhonglu An Zhisheng | 1998 | Chinese Science Bulletin1998，43，4： | 14 |
| 17 | Recent advances in cell sheet technology for bone and cartilage regeneration: from preparation to application显示文摘Bone defects caused by trauma,tumour resection,infection and congenital deformities,together with articular cartilage defects and cartilage–subchondral bone complex defects caused by trauma and degenerative diseases,remain great challenges for clinicians.Novel strategies utilising cell sheet technology to enhance bone and cartilage regeneration are being developed.The cell sheet technology has shown great clinical potential in regenerative medicine due to its effective preservation of cell–cell connections and extracellular matrix and its scaffold-free nature.This review will first introduce several widely used cell sheet preparation systems,including traditional approaches and recent improvements,as well as their advantages and shortcomings.Recent advances in utilising cell sheet technology to regenerate bone or cartilage defects and bone–cartilage complex defects will be reviewed.The key challenges and future research directions for the application of cell sheet technology in bone and cartilage regeneration will also be discussed. | Yuezhi Lu Wenjie Zhang Jie Wang Guangzheng Yang Shi Yin Tingting Tang Chunhua Yu Xinquan Jiang | 2019 | International Journal of Oral Science2019，11，2： | 14 |
| 18 | Optimization on the Impeller of a Low-specific-speed Centrifugal Pump for Hydraulic Performance Improvement显示文摘In order to widen the high-efficiency operating range of a low-specific-speed centrifugal pump, an optimization process for considering efficiencies under 1.0Q_d and 1.4Q_d is proposed. Three parameters, namely, the blade outlet width b_2, blade outlet angle β_2, and blade wrap angle φ, are selected as design variables. Impellers are generated using the optimal Latin hypercube sampling method. The pump efficiencies are calculated using the software CFX 14.5 at two operating points selected as objectives. Surrogate models are also constructed to analyze the relationship between the objectives and the design variables. Finally, the particle swarm optimization algorithm is applied to calculate the surrogate model to determine the best combination of the impeller parameters. The results show that the performance curve predicted by numerical simulation has a good agreement with the experimental results. Compared with the efficiencies of the original impeller, the hydraulic efficiencies of the optimized impeller are increased by 4.18% and 0.62% under 1.0Q_d and 1.4Q_d, respectively. The comparison of inner flow between the original pump and optimized one illustrates the improvement of performance. The optimization process can provide a useful reference on performance improvement of other pumps, even on reduction of pressure fluctuations. | PEI Ji WANG Wenjie YUAN Shouqi ZHANG Jinfeng | 2016 | Chinese Journal of Mechanical Engineering2016，29，5： | 13 |
| 19 | Three-dimensional bioprinting of multicell-laden scaffolds containing bone morphogenic protein-4 for promoting M2 macrophage polarization and accelerating bone defect repair in diabetes mellitus显示文摘Critical-sized bone defect repair in patients with diabetes mellitus remains a challenge in clinical treatment because of dysfunction of macrophage polarization and the inflammatory microenvironment in the bone defect region.Three-dimensional(3D)bioprinted scaffolds loaded with live cells and bioactive factors can improve cell viability and the inflammatory microenvironment and further accelerating bone repair.Here,we used modified bioinks comprising gelatin,gelatin methacryloyl(GelMA),and 4-arm poly(ethylene glycol)acrylate(PEG)to fabricate 3D bioprinted scaffolds containing BMSCs,RAW264.7 macrophages,and BMP-4-loaded mesoporous silica nanoparticles(MSNs).Addition of MSNs effectively improved the mechanical strength of GelMA/gelatin/PEG scaffolds.Moreover,MSNs sustainably released BMP-4 for long-term effectiveness.In 3D bioprinted scaffolds,BMP-4 promoted the polarization of RAW264.7 to M2 macrophages,which secrete anti-inflammatory factors and thereby reduce the levels of pro-inflammatory factors.BMP-4 released from MSNs and BMP-2 secreted from M2 macrophages collectively stimulated the osteogenic differentiation of BMSCs in the 3D bioprinted scaffolds.Furthermore,in calvarial critical-size defect models of diabetic rats,3D bioprinted scaffolds loaded with MSNs/BMP-4 induced M2 macrophage polarization and improved the inflammatory microenvironment.And 3D bioprinted scaffolds with MSNs/BMP-4,BMSCs,and RAW264.7 cells significantly accelerated bone repair.In conclusion,our results indicated that implanting 3D bioprinted scaffolds containing MSNs/BMP-4,BMSCs,and RAW264.7 cells in bone defects may be an effective method for improving diabetic bone repair,owing to the direct effects of BMP-4 on promoting osteogenesis of BMSCs and regulating M2 type macrophage polarization to improve the inflammatory microenvironment and secrete BMP-2. | Xin Sun Zhenjiang Ma Xue Zhao Wenjie Jin Chenyu Zhang Jie Ma Lei Qiang Wenhao Wang Qian Deng Han Yang Jinzhong Zhao Qianqian Liang Xiaojun Zhou Tao Li Jinwu Wang | 2021 | Bioactive Materials2021，6，3： | 12 |
| 20 | Structural basis of anti-PD-L1 monoclonal antibody avelumab for tumor therapy显示文摘 | Kefang Liu ShuguangTan Yan Chai Danqing Chen Hao Song Catherine Wei-Hong Zhang yi Shi Jun Liu Wenjie Tan Jianxin Lyu Shah Gao Jinghua Yan Jianxun Qi George F Gao | 2017 | Cell Research2017，27，1： | 11 |
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