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| 1 | The structural and accessory proteins M,ORF 4a,ORF 4b,and ORF 5 of Middle East respiratory syndrome coronavirus(MERS-CoV)are potent interferon antagonists显示文摘The newly emerged Middle East respiratory syndrome coronavirus(MERS-CoV)is a highly pathogenic respira-tory virus with pathogenic mechanisms that may be driven by innate immune pathways.The goal of this study is to characterize the expression of the structural(S,E,M,N)and accessory(ORF 3,ORF 4a,ORF 4b,ORF 5)proteins of MERS-CoV and to determine whether any of these pro-teins acts as an interferon antagonist.Individual structural and accessory protein-coding plasmids with an N-terminal HA tag were constructed and transiently transfected into cells,and their native expression and subcellular localiza-tion were assessed using Wes tern blotting and indirect immunofl uorescence.While ORF 4b demonstrated majorly nuclear localization,all of the other proteins demonstrated cytoplasmic localization.In addition,for the fi rst time,our experiments revealed that the M,ORF 4a,ORF 4b,and ORF 5 proteins are potent interferon antagonists.Further exami-nation revealed that the ORF 4a protein of MERS-CoV has the most potential to counteract the antiviral effects of IFN via the inhibition of both the interferon production(IFN-βpromoter activity,IRF-3/7 and NF-κB activation)and ISRE promoter element signaling pathways.Together,our re-sults provide new insights into the function and pathogenic role of the structural and accessory proteins of MERS-CoV. | Yang Yang Ling Zhang Heyuan Geng Yao Deng Baoying Huang Yin Guo Zhengdong Zhao Wenjie Tan | 2013 | Protein & Cell2013，4，12： | 25 |
| 2 | Study on shallow groundwater information extraction technology based on multi-spectral data and spatial data显示文摘Aimed at solving the difficulties,such as low efficiency and limited exploration range encountered in finding groundwater with the traditional methods,a new method was presented by using remote sensing technology in this paper.Based on multi-spectral data(ETM data) and spatial data(SRTM data),a forecasting model was built to produce a probability rating map for finding shallow groundwater in the arid and semi-arid areas.According to investigations,a conclusion is drawn that the results of the model are satisfied,which have been testified by the later geophysical exploration and drilling.Thus,the model can serve as a guide for finding groundwater in the arid and semi-arid regions. | YU DeHao DENG ZhengDong LONG Fan GUAN HongJun WANG DaQing GOU YiZheng | 2009 | Science China(Technological Sciences)2009，52，5： | 9 |
| 3 | Prevalence and molecular characteristics of Listeria monocytogenes in cooked products and its comparison with isolates from listeriosis cases显示文摘这研究试图在 Zigong 城市里在煮的产品调查流行和 Listeria monocytogenes 的分子的特征,中国。L 的全面出现。在商店和羊肉餐馆调查了的 ready-to-eat (RTE ) 的 monocytogenes 是 16.2%(141/873 ) 。13.5% 的出现在 RTE 猪肉被观察, 6.5% 在 RTE 蔬菜,并且非常, 24.0% 在也煮了羊肉或煮了将羊杂切碎加麦片纳入羊胃中煮成的食物。Serotype 1/2b (45.4%) , 1/2a (33.3%) ,和 1/2c (14.2%) 是占优势的类型。由基于 L 的打字的 multilocus 顺序(MLST ) 比较同种细胞的建筑群(CC ) 。从从中国, CC87, CC9, CC8,和 CC3 的不同区域的 Zigong 城市和 33 个 listeriosis 案例里的煮的食物的 monocytogenes 被显示在煮的产品和 CC87 流行, CC3 是在 33 诊所来源紧张的开始的二种经常的类型。所有 CC87 污点怀有最新报导的 Listeria 致病力岛 4 (LIPI-4 ) 基因碎片 ptsA,和所有 CC3 紧张拥有了 Listeria 致病力岛 3 (LIPI-3 ) 基因碎片 llsX。这些可以增加在中国在 listeriosis 情况中属于 CC87 和 CC3 的紧张的出现并且也从 Zigong 由于煮的产品的消费加重感染的风险。ST619 (serotype 1/2b ) 怀有 llsX 和 ptsA,显示在 Zigong 的一种潜在的 hypervirulent 顺序类型。 | Hong Wang Lijuan Luo Zhengdong Zhang Jianping Deng Yan Wang Yimao Miao Ling Zhang Xi Chen Xiang Liu Songsong Sun Bo Xiao Qun Li Changyun Ye | 2018 | Frontiers of Medicine2018，12，1： | 9 |
| 4 | Numerical simulation on the dynamic response of buried pipelines subjected to blast loads 显示文摘 | XU Guofu DENG Zhengdong DENG Feifan | 2013 | Advanced Materials Research2013，，： | 1 |
| 5 | Retrieval study of lake water depth by using multi-spectral remote sensing in Bangong Co Lake显示文摘This paper reviewed studies on remote sensing of water depth retrieval. Four water depth retrieval models(single-band, double-ratio-band, multi-band, and BP network models) were evaluated using TM image and water data from Bangong Co Lake, which is located in China's Tibet Autonomous Region and Indian Kashmir. Tested by independent data, comparison of these four models demonstrates that BP network model performed better than the multi-band model, with the single-band model performing the worst. To sum up, this study demonstrates that first, BP network model performed better than the traditional model; second, precise atmospheric correction and radiation study, affected by different water level sand sediment, could improve the precision of water depth retrieval. | Pu Zheng ZhengDong Deng Xin Ye | 2014 | Research in Cold and Arid Regions2014，6，3： | 0 |
| 6 | Structural insights into plant phytochrome A as a highly sensitized photoreceptor显示文摘Dear Editor,Phytochromes(phys),first discovered in plants,are red and far-red photoreceptors that are also widely found in bacteria and fungi later.1,2 Plant phys utilize the linear tetrapyrrole chromophore called photochromobilin(PΦB).Phys reversibly toggle between the red light-absorbing Pr and the far-red light-absorbing Pfr conformers by photoconversion,with absorption peaks at~667 nm and~730 nm,respectively.The Pfr conformer can be converted to Pr in darkness,a process called dark reversion or thermoreversion. | Yuxuan Zhang Xiaoli Lin Chengying Ma Jun Zhao Xiaojin Shang Zhengdong Wang Bin Xu Ning Gao Xing Wang Deng Jizong Wang | 2023 | Cell Research2023，33，10： | 0 |
| 7 | Asymmetric Hydrogenation of Tetrasubstituted α,β-Unsaturated Ketones:Access to Chiral 2-Substituted Cyclopentyl Aryl Ketones显示文摘Asymmetric hydrogenation of tetrasubstituted alkenes is an important but challenging research topic.Herein,we report an efficient iridium-catalyzed asymmetric hydrogenation of tetrasubstituted α,β-unsaturated ketones for the synthesis of chiral 2-substituted cyclopentyl aryl ketones,an important chiral structural motif for the preparation of chiral pharmaceuticals and bioactive molecules.The reaction proceeded very well with good functional group compatibility and delivered the hydrogenated products in high yields and stereoselectivities(up to 99% yield,>20:1 dr and 99%ee).In addition,the reaction could be carried out on a gram-scale,and all four stereoisomers of the hydrogenated products bearing two contiguous stereocenters were obtained.Furthermore,the hydrogenated product can be transformed into the ERβ agonist Erteberel,and the reaction pathway was also studied via deuterium-labelling experiments. | Zhengdong Ding Feng Gao Yining Lu Qianjia Yuan Wei-Ping Deng Wanbin Zhang | 2023 | Precision Chemistry2023，1，3： | 0 |
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