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| 1 | In vitro antibacterial activity of thymol and carvacrol and their effects on broiler chickens challenged with Clostridium perfringens显示文摘Background:In the post-antibiotic era,essential oils(EO) are promising alternatives to growth-promoting antibiotics.The aim of the present study was to investigate the antibacterial activities of an EO product and its components thymol and carvacrol in vitro,and the efficacy of EO to control Clostridium perfringens challenge in broiler chickens.Results:The in vitro minimum inhibitory concentration assay showed strong antibacterial activity of the EO product,thymol,and carvacrol against pathogenic Escherichia coli,C.perfringens,and Salmonella strains,and weak activity towards beneficial Lactobacillus strains.Besides,an additive effect was observed between thymol and carvacrol.The in vivo study was carried out with 448 male broiler chicks following a 4 × 2 factorial arrangement to test the effects of EO supplementation(0,60,120,or 240 mg/kg EO in wheat-based diet),pathogen challenge(with or without oral gavage of C perfringens from day 14 to day 20) and their interactions.Each treatment consisted of eight replicate pens(seven birds/pen).The challenge led to macroscopic gut lesions,and resulted in a significant increase in ileal populations of C.perfringens and Escherichia subgroup(P< 0.05) on day 21.Dietary EO supplementation did not influence C perfringens numbers,but linearly alleviated intestinal lesions on day 21 and 28(P=0.010 and 0.036,respectively),and decreased Escherichia populations in ileum with increased EO dosages(P=0.027 and 0.071 for day 21 and 28,respectively).For caecum,EO quadratically influenced Lactobacillus populations on day 21(p=0.002),and linearly decreased the numbers of total bacteria and Escherichia on day 28(P=0.026 and 0.060,respectively).Mean thymol and carvacrol concentrations in the small intestine were 0.21 and 0.20 μg/g in intestinal digesta(wet weight),respectively,for birds fed 60 mg/kg EO,and 0.80 and 0.71 μg/g,respectively,for birds fed 240 mg/kg EO.Conclusions:These results indicated that dietary EO supplementation could affect intestinal microbiota and alleviate intestinal lesions in broilers,which may contribute in controlling C.perfringens infection in broiler chickens. | Encun Du Liping Gan Zhui Li Weiwei Wang Dan Liu Yuming Guo | 2016 | Journal of Animal Science and Biotechnology2016，7，1： | 14 |
| 2 | N-acetylcysteine alleviates angiotensin II-mediated renal fibrosis in mouse obstructed kidneys显示文摘瞄准:为了在血管收缩素 II (Ang II ) 上调查 ROS scavenger N-acetylcysteine (NAC ) 的效果,调停了在 vivo 并且在 vitro.Methods 的肾的纤维变性:老鼠受到单方的 ureteral 阻塞(UUO ) ,然后与车辆或 NAC 对待(250 mg/kg, ip ) 为 7 天。妨碍的肾的组织学的变化与 Massons 被观察三色的染色。ROS 层次被检测, DHE 染色。在妨碍的肾的相关蛋白质的表示用西方的弄污的试金被估计。肾的成纤维细胞 NRK-49F 房间在 vitro experiments.Results 被用于的有教养的老鼠:在妨碍的肾, Ang II 层次显著地被提高,并且骨胶原我在空隙的空格被积累。而且, ROS 生产和 p47 (NADPH oxidase 建筑群的一个关键子单元) 的表示以一种时间依赖者方式被增加;fibronectin 的表示, α -SMA 和 TGF-β是 upregulated。NAC 的管理显著地在妨碍的肾减轻了 fibrotic 回答。在有教养的 NRK-49F 房间,有 Ang II 的处理(0.001-10 μ mol/L ) 增加了 fibronectin 的表示,骨胶原我, α -SMA 和 TGF-β在剂量依赖者和时间依赖者礼貌。Ang II 也增加了 ROS 生产和 Smad3 的 phosphorylation。有 NAC 的预告的处理(5 μ mol/L ) 在 cells.Conclusion 的堵住的 Ang 导致 II 的氧化压力和 ECM 生产:在老鼠妨碍了肾,从 Ang II upregulation 的 fibrotic 回答结果能被 ROS scavenger N-acetylcysteine 减轻。 | Yang SHEN Nai-jun MIAO Jin-lan XU Xin-xin GAN Dan XU Li ZHOU Hong XUE Wei ZHANG Li-min LU | 2016 | Acta Pharmacologica Sinica2016，37，5： | 7 |
| 3 | Toxicity of Graphene Quantum Dots in Zebrafish Embryo显示文摘Objective To evaluate the bio-safety of graphene quantum dots(GQDs), we studied its effects on the embryonic development of zebrafish. Methods In vivo, biodistribution and the developmental toxicity of GQDs were investigated in embryonic zebrafish at exposure concentrations ranging from 12.5-200 μg/m L for 4-96 h post-fertilization(hpf). The mortality, hatch rate, malformation, heart rate, GQDs uptake, spontaneous movement, and larval behavior were examined. Results The fluorescence of GQDs was mainly localized in the intestines and heart. As the exposure concentration increased, the hatch and heart rate decreased, accompanied by an increase in mortality. Exposure to a high level of GQDs(200 μg/mL) resulted in various embryonic malformations including pericardial edema, vitelline cyst, bent spine, and bent tail. The spontaneous movement significantly decreased after exposure to GQDs at concentrations of 50, 100, and 200 μg/mL. The larval behavior testing(visible light test) showed that the total swimming distance and speed decreased dose-dependently. Embryos exposed to 12.5 μg/mL showed hyperactivity while exposure to higher concentrations(25, 50, 100, and 200 μg/mL) caused remarkable hypoactivity in the light-dark test. Conclusion Low concentrations of GQDs were relatively non-toxic. However, GQDs disrupt the progression of embryonic development at concentrations exceeding 50 μg/mL. | WANG Zhen Guo ZHOU Rong JIANG Dan SONG Jing E XU Qian SI Jing CHEN Yun Ping ZHOU Xin GAN Lu LI Jian Zhen ZHANG Hong LIU Bin | 2015 | Biomedical and Environmental Sciences2015，28，5： | 6 |
| 4 | SND p102 promotes extracellular matrix accumulation and cell proliferation in rat glomerular mesangial cells via the ATIR/ERK/Smad3 pathway显示文摘 | Jin-lan XU Xin-xin GAN Jun NI De-cui SHAO Yang SHEN Nai-jun MIAO Dan XU Li ZHOU Wei ZHANG Li- min LU | 2018 | Acta Pharmacologica Sinica2018，39，9： | 5 |
| 5 | Urban-rural disparities in mild cognitive impairment and its functional subtypes among community- dwelling older residents in central China显示文摘background Substantial variations in the prevalence of mild cognitive impairment(MCI)and its subtypes have been reported,although mostly in geographically defined developed countries and regions.Less is known about MCI and its subtypes in rural areas of less developed central China.Aims The study aimed to compare the prevalence of MCI and its subtypes in residents aged 65 years or older in urban and rural areas of Hubei Province,China.Methods Participants aged 65 years or older were recruited between 2018 and 2019.Inperson structured interviews and clinical and neuropsychological assessments were performed at city health community centres and township hospitals.results Among 2644 participants without dementia,735 had MCI,resulting in a prevalence of 27.8%for total MCI,20.9%for amnestic MCI(aMCI)and 6.9%for non-amnestic MCI(naMCI).The prevalence of MCI in urban and rural areas was 20.2%and 44.1%,respectively.After adjusting for demographic factors,the prevalence of total MCI,aMCI and naMCI differed significantly between rural and urban areas(adjusted odds ratio(OR)2.10,1.44 and 3.76,respectively).Subgroup analysis revealed an association between rural socioeconomic and lifestyle disadvantage and MCI and its subtypes.Conclusions Our findings suggest that the prevalence of MCI among urban residents in central China is consistent with that in other metropolis areas,such as Shanghai,but the prevalence in rural areas is twice that in urban areas.Prospective studies and dementia prevention in China should focus on rural areas. | Dan Liu Lin Li Lina An Guirong Cheng Cong Chen Mingjun Zou Bo Zhang Xuguang Gan Lang Xu Yangming Ou Qingming Wu Ru Wang Yan Zeng | 2021 | General Psychiatry2021，34，5： | 3 |
| 6 | Fiber-tip polymer clamped-beam probe for high-sensitivity nanoforce measurements显示文摘Micromanipulation and biological,material science,and medical applications often require to control or measure the forces asserted on small objects.Here,we demonstrate for the first time the microprinting of a novel fiber-tip-polymer clamped-beam probe micro-force sensor for the examination of biological samples.The proposed sensor consists of two bases,a clamped beam,and a force-sensing probe,which were developed using a femtosecond-laser-induced two-photon polymerization(TPP)technique.Based on the finite element method(FEM),the static performance of the structure was simulated to provide the basis for the structural design.A miniature all-fiber micro-force sensor of this type exhibited an ultrahigh force sensitivity of 1.51 nmμN−1,a detection limit of 54.9 nN,and an unambiguous sensor measurement range of~2.9 mN.The Young’s modulus of polydimethylsiloxane,a butterfly feeler,and human hair were successfully measured with the proposed sensor.To the best of our knowledge,this fiber sensor has the smallest force-detection limit in direct contact mode reported to date,comparable to that of an atomic force microscope(AFM).This approach opens new avenues towards the realization of small-footprint AFMs that could be easily adapted for use in outside specialized laboratories.As such,we believe that this device will be beneficial for high-precision biomedical and material science examination,and the proposed fabrication method provides a new route for the next generation of research on complex fiber-integrated polymer devices. | Mengqiang Zou Changrui Liao Shen Liu Cong Xiong Cong Zhao Jinlai Zhao Zongsong Gan Yanping Chen Kaiming Yang Dan Liu Ying Wang Yiping Wang | 2021 | Light(Science & Applications)2021，10，9： | 3 |
| 7 | Regulation of Leaf Longevity by DML3-Mediated DNA Demethylation显示文摘Leaf senescence is driven by the expression of senescence-associated genes(SAGs).Development-specific genes often undergo DNA demethylation in their promoter and other regions,which regulates gene expression.Whether and how DNA demethylation regulates the expression of SAGs and thus leaf senescence remain elusive.Whole-genome bisulfite sequencing(WGBS)analyses of wild-type(WT)and demeter-like 3(dml3)Arabidopsis leaves at three developmental stages revealed hypermethylation during leaf senescence in dml3 compared with WT,and 20556 differentially methylated regions(DMRs)were identified by comparing the methylomes of dml3 and WT in the CG,CHG,and CHH contexts.Furthermore,we identified that 335 DMR-associated genes(DMGs),such as NAC016 and SEN1,are upregulated during leaf senescence,and found an inverse correlation between the DNA methylation levels(especially in the promoter regions)and the transcript abundances of the related SAGs in WT.In contrast,in dml3 the promoters of SAGs were hypermethylated and their transcript levels were remarkably reduced,and leaf senescence was significantly delayed.Collectively,our study unraveled a novel epigenetic regulatory mechanism underlying leaf senescence in which DML3 is expressed at the onset of and during senescence to demethylate promoter,gene body or 3'UTR regions to activate a set of SAGs. | Lu Yuan Dan Wang Liwen Cao Ningning Yu Ke Liu Yongfeng Guo Susheng Gan Liping Chen | 2020 | Molecular Plant2020，13，8： | 3 |
| 8 | Characteristics of SARS-CoV-2 Delta variant-infected individuals with intermittently positive retest viral RNA after discharge显示文摘SARS-CoV-2-infected patients will be discharged when viral ribonucleic acid(RNA)is consecutively negative at least twice over a 24-h interval.However,we have demonstrated that positive retest viral RNA,a particular clinical phenomenon initially observed in discharged SARS-CoV-2-infected patients in early 2020[1,2],is the occasional reoccurrence of Polymerase Chain Reaction(PCR)-detectable viral RNA but not reinfection[3]. | Lu Li Jingyan Tang Zhiwei Xie Qingxin Gan Guofang Tang Zhongwei Hu Huimin Zeng Jingrong Shi Jiaojiao Li Yan Li Changwen Ke Min Kang Dan Liang Huan Lu Yuwei Tong Xilong Deng Jinxin Liu Hongzhou Lu Fuxiang Wang Fengyu Hu Feng Li Nanshan Zhong Xiaoping Tang | 2022 | National Science Review2022，9，10： | 3 |
| 9 | Seismic behavior of thin-walled circular and stiffened square steel tubed-reinforced-concrete columns显示文摘Steel tubed-reinforced-concrete(TRC) columns have been gradually used in the construction of high-rise buildings recently because of their high axial load-carrying capacities and excellent seismic behavior. Existing studies about their seismic behavior were focused on columns with relatively thick tubes, i.e., diameter-to-thickness/width-to-thickness(D/t) ratios were below 100,while little is known about thin-walled TRC columns, especially for square TRC columns. Considering the infilled concrete of square TRC columns is non-uniformly and non-effectively confined, accordingly, stiffened square TRC columns are usually adopted in practice. Thus, two thin-walled circular TRC columns(D/t=120) and two stiffened square ones with diagonal stiffeners in plastic hinge regions(D/t=106) were tested under a constant axial compression combined with cyclic lateral loading.Both the circular and stiffened square TRC columns had the same cross sectional area, tube thickness, reinforcing bar ratio and column height. Flexural failure occurred for all the four specimens. Test results showed the strengths of the stiffened square TRC columns were a little higher in comparison to their circular counterparts; the ductility and energy dissipation capacities were excellent for both the stiffened and circular TRC columns, indicating very good confinement was gained from the yielded steel tubes of the plastic hinge regions at the peak loads. And shear stresses(35–90 MPa) in the sheared plates showed their moderate contribution of carrying lateral loads. Finally, cross sectional capacity analysis results demonstrated the method for TRC columns is acceptable for the stiffened square TRC columns. | GAN Dan ZHOU XuHong LIU JiePeng LI Jiang | 2019 | Science China(Technological Sciences)2019，62，3： | 3 |
| 10 | A low complexity algorithm of blind estimation of convolutional interleaver parameters显示文摘Interleaving plays an important role in many wireless communication systems,which is used to cope with burst errors and improve the reliability of data transmission.In this paper,we propose a new method to blindly estimate the parameters of convolutional interleaver in a non-cooperative context.The proposed method exploits both the property of interleaved stream and the deinterleaving technique.The product of the depth and width of the interleaver is firstly determined by analyzing interleaved data stream.Then the interleaved data stream is let to pass through a series of reconstructed deinterleavers.Thus the delay,the depth and width of the convolutional interleaver can be estimated by analyzing the deinterleaved data streams.Compared with other algorithms,the proposed method significantly reduces the searching scope and improves computational efficiency.Simulation results show that the algorithm has good performance even if errors exist. | GAN Lu LI Dan LIU ZongHui LI LiPing | 2013 | Science China(Information Sciences)2013，56，4： | 2 |
| 11 | Cellular Tolerance, Accumulation and Distribution of Cadmium in Leaves of Hyperaccumulator Picris divaricata显示文摘Knowledge of cellular metal homeostasis will provide a better understanding of the mechanisms involved in metal tolerance and hyperaccumulation in metal-hyperaccumulating plants. Energy dispersive X-ray spectrometry (EDS) was used to determine the localization of cadmium (Cd) in leaves of the Zn/Cd hyperaccumulator Picris divaricata which had a shoot Cd concentration of 565 mg kg 1 after 2 weeks of growth in solution culture supplying 10 μmol L-1 CdCl2 . The results indicated that Cd was distributed mainly in the trichomes, upper and lower epidermis and bundle sheath cells, with a relatively low level of Cd in mesophyll cells. Mesophyll protoplasts isolated from leaves remained viable after 24 h exposure to CdCl2 at a concentration up to 1 mmol L-1 , indicating their high tolerance to Cd. The intracellular Cd was visualized by staining with Leadmium Green dye, a cellular permeable Cd fluorescence probe. The results showed that the majority of protoplasts (> 82%) did not accumulate Cd, with only a minority (< 18%) showing Cd accumulation. In the Cd-accumulating protoplasts, Cd accumulation was depressed by the addition of Fe2+ , Mn2+ and the metabolic inhibitor carbonyl cyanide m-chlorophenylhydrazone (CCCP), but not by Ca 2+ or Zn2+ . Furthermore, the entire process of Cd uptake from external solution into the cytoplasm and subsequent sequestration into vacuoles was successfully recorded by confocal images. These results suggested that reduced cellular Cd accumulation and efficient Cd vacuolar sequestration in mesophyll cells might be responsible for cellular Cd tolerance and distribution in the leaves of P. divaricata. | HU Peng-Jie GAN Yuan-Yuan TANG Ye-Tao ZHANG Quan-Fang JIANG Dan YAO Nan QIU Rong-Liang | 2012 | Pedosphere2012，22，4： | 2 |
| 12 | Diagnosis,treatment,and prevention of severe acute hepatitis of unknown etiology in children显示文摘Background Severe acute hepatitis of unknown etiology in children has recently exhibited a global trend of concentrated occurrence.This review aimed to summarize the current available information regarding the outbreak of severe acute hepatitis and introduce our hospital’s previous experiences with the diagnosis and treatment of severe acute hepatitis for reference.Data sources Websites including the UK Health Security Agency,European Centre for Disease Prevention and Control,CDC,WHO,and databases including PubMed/Medline,Cochrane Library,Embase and Web of Science were searched for articles on severe acute hepatitis in children.Results As of May 26,2022,a total of 650 cases have been reported in 33 countries;at least 38(6%)children required liver transplantation,and nine(1%)died.Cases are predominantly aged between 3 and 5 years old,and there are no epidemiological links among them.The common manifestations are jaundice,vomiting and pale stools.Adenovirus tested positive in most cases,and SARS-CoV-2 and other viruses were detected in a few cases,but virus particles were not found in liver tissue.Adenovirus immunohistochemistry showed immunoreactivity in the intrasinusoidal lumen from some liver samples.The hierarchical treatment includes symptomatic and supportive therapy,management of coagulation disorders and hepatic encephalopathy,artifcial liver support,and liver transplantation(approximately 6%–10%of cases require liver transplant).Conclusions The etiology of this severe acute hepatitis in children is not clear.The clinical features are severe acute hepatitis with signifcantly elevated liver enzymes.Clinicians need to be alert to children with hepatitis. | Ying‑Hu Chen Jin‑Gan Lou Zi‑Hao Yang Qing‑Jiang Chen Chun‑Zhen Hua Sheng Ye Chen‑Mei Zhang Jie Chen Zong‑Wei Huang Jin‑Dan Yu Zhi‑Gang Gao Qiang Shu | 2022 | World Journal of Pediatrics2022，18，8： | 2 |
| 13 | A Reduced Reachability Tree for a Class of Unbounded Petri Nets显示文摘As a powerful analysis tool of Petri nets, reachability trees are fundamental for systematically investigating many characteristics such as boundedness, liveness and reversibility.This work proposes a method to generate a reachability tree,called ω RT for short, for a class of unbounded generalized nets called ω-independent nets based on new modified reachability trees(NMRTs). ω RT can effectively decrease the number of nodes by removing duplicate and ω-duplicate nodes in the tree, and verify properties such as reachability, liveness and deadlocks.Two examples are provided to show its superiority over NMRTs in terms of tree size. | Shouguang Wang Mengdi Gan Mengchu Zhou Dan You | 2015 | IEEE/CAA Journal of Automatica Sinica2015，2，4： | 2 |
| 14 | Clinical features and risk factors for post-partum depression in a large cohort of Chinese women with recurrent major depressive disorder显示文摘 | Tian Tian Yihan Li Dong Xie Yifeng Shen Jianer Ren Wenyuan Wu Chengbin Guan Zhen Zhang Danning Zhang Chengge Gao Xiaoming Zhang Jinbo Wu Hong Deng Gang Wang Yunshu Zhang Yun Shao Han Rong Zhaoyu Gan Yan Sun Bin Hu Jiyang Pan Yi Li Shufan Sun Libo Song Xue | 2011 | Journal of Affective Disorders2011，，3： | 1 |
| 15 | Extraction optimized by using response surface methodology,purification and preliminary characterization of polysaccharides from Hyriopsis cumingii显示文摘 | Qiao Deliang Hu Bing Gan Dan | | 0，，03： | 1 |
| 16 | Effects of flavonoid glycosides obtained from a Ginkgo biloba extract fraction on the physical and oxidative stabilities of oil-in-water emulsions prepared from a stripped structured lipid with a low omega-6 to omega-3 ratio显示文摘 | YANG Dan WANG Xiangyu GAN Lujing | 2015 | Food Chemistry2015，174，： | 1 |
| 17 | Extraction optimized by using response surface methodology, purification and preliminary characterization of polysaccharides from Hyriopsis cumingii显示文摘 | QIAO Deliang HU Bing GAN Dan | 2009 | Carbohydrate Polymers2009，76，： | 1 |
| 18 | Orientation and Motion of Water Molecules at Air/Water Interface显示文摘这里,我们在空气 / 水接口报导 orientational 结构的量的研究和水分子的运动。和频率产生(SFG ) 的分析震动的山峰免费哦在四种试验性的配置在 3700 厘米 1 拉长乐队在空气 / 水显示出水分子的那个 orientational 运动接口在一个有限尖范围以内是摆动的。免费哦界面的水分子的债券在 33 附近被倾斜??啓???￣??啓??????????啓?￣橩?匯?????????瘠?啓??匯??瘠?啓??匯??瘠?啓??匯????匼???啓?????牡楬杮?湥楮潳??柳?? | Wei Gan Dan Wu Zhen Zhang Yuan Guo Hong-fei Wang | 2006 | Chinese Journal of Chemical Physics2006，19，1： | 1 |
| 19 | Seismic behavior and moment strength of tubed steel reinforced-concrete (SRC) beam-columns 显示文摘 | Dan Gan Lanhui Guo Jiepeng | 2011 | Journal of Constructional Steel Research2011，67，： | 1 |
| 20 | Extraction optimized by using response surface methodology, purification and preliminary char- actedzation of polysaccharides from Hyriopsis cumingii显示文摘 | QIAO Deliang HUA Bing GAN Dan | 2009 | Carbohy- drate Polymers2009，76，3： | 1 |
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