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| 1 | Expression of matrix metalloproteinases 2 and 9 in human gastric cancer and superficial gastritis显示文摘AIM:To assess expression of matrix metalloproteinases 2(MMP2)and MMP9 in gastric cancer,superficial gastritis and normal mucosa,and to measure metalloproteinase activity.METHODS:MMP2 and MMP9 mRNA expression was determined by quantitative real-time polymerase chain reaction.Normalization was carried out using three different factors.Proteins were analyzed by quantitative gelatin zymography(qGZ).RESULTS:18S ribosomal RNA(18SRNA)was very highly expressed,while hypoxanthine ribosyltransferase-1(HPRT-1)was moderately expressed.MMP2 was highly expressed,while MMP9 was not detected or lowly expressed in normal tissues,moderately or highly expressed in gastritis and highly expressed in cancer.Relative expression of 18SRNA and HPRT-1 showed no significant differences.Significant differences in MMP2 and MMP9 were found between cancer and normal tissue,but not between gastritis and normal tissue.Absolute quantification of MMP9 echoed this pattern,but differential expression of MMP2 proved conflictive.Analysis by qGZ indicated significant differences between cancer and normal tissue in MMP-2,total MMP-9,250 and 110 kDa bands.CONCLUSION:MMP9 expression is enhanced in gastric cancer compared to normal mucosa;interpretation of differential expression of MMP2 is difficult to establish. | Clara Luz Sampieri Sol de la Pea Mariana Ochoa-Lara Roberto Zenteno-Cuevas Kenneth León-Córdoba | 2010 | World Journal of Gastroenterology2010，16，12： | 46 |
| 2 | Effects of chronic acarbose treatment on adipocyte insulin responsiveness, serum levels of leptin and adiponectin and hypothalamic NPY expression in obese diabetic Wistar rats显示文摘 | Perez C Fern a ndez-Agull o T De Sol i s AJ | 2008 | Clin Exp Pharmacol Physiol2008，35，3： | 1 |
| 3 | Multiplexed Biosensing Diagnostic Platforms Detecting Autoantibodies to Tumor-Associated Antigens from Exosomes Released by CRC Cells and Tissue Samples Showed High Diagnostic Ability for Colorectal Cancer显示文摘Colorectal cancer(CRC)is the second leading cause of cancer-related death worldwide.The five-year survival rate of CRC patients depends on the stage at diagnosis,being higher than 80%when CRC is diagnosed in the early stages but lower than 10%when CRC is diagnosed in advanced stages.Autoantibodies against specific CRC autoantigens(tumor-associated antigens(TAAs))in the sera of patients have been widely demonstrated to aid in early diagnosis.Thus,we herein aim to identify autoantigens target of autoantibodies specific to CRC that possess a significant ability to discriminate between CRC patients and healthy individuals by means of liquid biopsy.To that end,we examined the protein content of the exosomes released by five CRC cell lines and tissue samples from CRC patients by means of immunoprecipitation coupled with mass spectrometry analysis.A total of 103 proteins were identified as potential autoantigens specific to CRC.After bioinformatics and meta-analysis,we selected 15 proteins that are more likely to be actual CRC autoantigens in order to evaluate their role in CRC prognosis by Western blot(WB)and immunohistochemistry(IHC).We found dysregulation at the protein level for 11 of these proteins in both tissue and plasma exosome samples from patients,along with an association of nine of these proteins with CRC prognosis.After validation,all but one showed a statistically significant high diagnostic ability to distinguish CRC patients and individuals with premalignant lesions from healthy individuals,either by luminescence Halotag-based beads,or by a multiplexed biosensing platform involving the use of magnetic microcarriers as solid support modified with covalently immobilized Halotag fusion proteins constructed for CRC detection.Taken together,our results highlight the usefulness of the approach defined here to identify the TAAs specific to chronic diseases;they also demonstrate that the measurement of autoantibody levels in plasma against the TAAs identified here could be integrated into a point-of-care(POC)device for CRC detection with high diagnostic ability. | Ana Montero-Calle Itziar Aranguren-Abeigon María Garranzo-Asensio Carmen Poves María Jesús Fernández-Aceñero Javier Martínez-Useros Rodrigo Sanz Jana Dziaková Javier Rodriguez-Cobos Guillermo Solís-Fernández Eloy Povedano Maria Gamella Rebeca Magnolia Torrente-Rodríguez Miren Alonso-Navarro Vivian de los Ríos J.Ignacio Casal Gemma Domínguez Ana Guzman-Aranguez Alberto Peláez-García JoséManuel Pingarrón Susana Campuzano Rodrigo Barderas | 2021 | Engineering2021，7，10： | 1 |
| 4 | Carotid intima-media thickness at different sites: relation to incident myocardial infarction; The Rotterdam Study显示文摘 | Iglesias del Sol A Bots ML Grobbee DE | 2002 | Eur Heart J2002，23，12： | 1 |
| 5 | Laparoscopic adhesiolysis in acute small bowel obstruction显示文摘 | Cirocehi R Giustozzi G De Sol A | 2007 | Minerva Chirurgica2007，62，6： | 1 |
| 6 | Carotid intima-media thickness at different sites:Relation to incident myocardial infarction:The Rotterdam Study显示文摘 | Iglesias del Sol A Bots ML Grobbee DE | 2002 | Eur Heart J2002，23，12： | 1 |
| 7 | Differential MiRNA expression in childhood acute lymphoblastic leukemia and association with clinical and biological features显示文摘 | Jaqueline Carvalho de Oliveira Carlos Alberto Scrideli María Sol Brassesco Andressa Gois Morales Julia Alejandra Pezuk Rosane de Paula Queiroz José Andres Yunes Silvia Regina Brandalise Luiz Gonzaga Tone | 2011 | Leukemia Research2011，，3： | 1 |
| 8 | Caroid intima-media thickness at different sites : relation to incident myocardial infarction; The Rotteram study 显示文摘 | Iglesias del Sol A Grobbee DE Hofman A | 2002 | Eur Heart J2002，23，12： | 1 |
| 9 | Carotid intima - media thickness at different sites: relation to incident myocardial infar- action; The Rotterdam Study 显示文摘 | Iglesias del Sol A Bots ML Grobbee DE | 2002 | Eur Heart J2002，23，12： | 1 |
| 10 | New real-time PCR able to detect in a single tube multiple rifampin resistance mutations in Mycobacerium tuberculosis显示文摘 | Garc'a de Viedma D del Sol Díaz Infantes M Lasala F | 2002 | J Clin Microbiol2002，40，3： | 1 |
| 11 | Carotid intima-media thickness at different sites:relation to incident myocardial infarction;the Rotterdam study显示文摘 | Iglesias del Sol A Bots ML Grobbee DE | 2002 | Eur Heart J2002，23，12： | 1 |
| 12 | The measurement ofamylase in drain fluid for the detention of pancreatic fistula aftergastric cancer surgery: an interim analysis显示文摘 | De Sol A Cirocchi R Di Patrizi MS | 2015 | World J Surg On-col2015，13，： | 1 |
| 13 | Steroid withdrawal in lung transplant recipients显示文摘 | Borro JM Solé A De la Torre M | | 0，，09： | 1 |
| 14 | RAPD variation in wild population off our species of the genus Hordeum显示文摘 | DE BUSTOS A CASANOVA C SOL ER C | 1998 | Theor Appl Genet1998，，96： | 1 |
| 15 | Carotid intima media thickness at different sites: Relation to incident myocardial infarction: The Rotterdam Study 显示文摘 | Iglesias del Sol A Bots ML Grobbee DE | 2002 | Eur Heart J2002，23，12： | 1 |
| 16 | Segmentation of confocal microscope images of cell nuclei in thick tissue sections显示文摘 | C.ORTIZ DE SOLóRZANO E.GARCíA RODRIGUEZ A.JONES D.PINKEL J. W.GRAY D.SUDAR S. J.LOCKETT | 2003 | Journal of Microscopy2003，，3： | 1 |
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