|
|
|
题名
|
作者
|
年代
|
出处
|
被引量
|
| 1 | Differentiation of various tra- ditional Chinese medicines derived from animal bile and gallstone: simultaneous determination of bile acids by liquid chromatography cou- pled with triple quadrupole mass spectrometry 显示文摘 | Qiao Xue Ye Min Pan Delin | 2011 | Journal of Chro- matography A2011，1218，1： | 1 |
| 2 | Inter-industry Technology Spillover Effects in China:Evidence from 35 Industrial Sectors显示文摘The present paper calculates 35 industrial sectors' similarity matrices for the period of 1997-2008 using China's input-output tables for 1997,2002 and 2007,and uses these to measure inter-industry technology spillover to analyze the spillover effects on industrial sectors' labor productivity.The empirical analysis shows that inter-industry technology spillover has a significant positive effect on the labor productivity of each industry.The elasticity of productivity effects of inter-industry technology spillover is not only larger than that of direct R&D input,but also increases over time.We group the industries into four major categories and find that the inter-industry technology spillover effect within the categories is,on average,greater than that between the four categories,indicating that technology spillover occurs more easily between similar industries.This research shows that the interindustry technology spillover effect in China has begun to increase,and the government should take advantage of this effect. | Wenqing Pan Delin Yang Min Lin | 2012 | China & World Economy2012，20，2： | 1 |
| 3 | Single-base editing in IGF2 improves meat production and intramuscular fat deposition in Liang Guang Small Spotted pigs显示文摘Background Chinese indigenous pigs are popular with consumers for their juiciness,flavour and meat quality,but they have lower meat production.Insulin-like growth factor 2(IGF2) is a maternally imprinted growth factor that promotes skeletal muscle growth by regulating cell proliferation and differentiation.A single nucleotide polymorphism(SNP) within intron 3 of porcine IGF2 disrupts a binding site for the repressor,zinc finger BED-type containing 6(ZBED6),leading to up-regulation of IGF2 and causing major effects on muscle growth,heart size,and backfat thickness.This favorable mutation is common in Western commercial pig populations,but absent in most Chinese indigenous pig breeds.To improve meat production of Chinese indigenous pigs,we used cytosine base editor 3(CBE3)to introduce IGF2 intron3-C3071T mutation into porcine embryonic fibroblasts(PEFs) isolated from a male Liang Guang Small Spotted pig(LGSS),and single-cell clones harboring the desired mutation were selected for somatic cell nuclear transfer(SCNT) to generate the founder line of IGF2^(T/T) pigs.Results We found the heterozygous progeny IGF2^(C/T) pigs exhibited enhanced expression of IGF2,increased lean meat by 18%-36%,enlarged loin muscle area by 3%-17%,improved intramuscular fat(IMF) content by 18%-39%,marbling score by 0.75-1,meat color score by 0.53-1.25,and reduced backfat thickness by 5%-16%.The enhanced accumulation of intramuscular fat in IGF2^(C/T) pigs was identified to be regulated by the PI3K-AKT/AMPK pathway,which activated SREBP1 to promote adipogenesis.Conclusions We demonstrated the introduction of IGF2-intron3-C3071T in Chinese LGSS can improve both meat production and quality,and first identified the regulation of IMF deposition by IGF2 through SREBP1 via the PI3KAKT/AMPK signaling pathways.Our study provides a further understanding of the biological functions of IGF2and an example for improving porcine economic traits through precise base editing. | Tianqi Duo Xiaohong Liu Delin Mo Yu Bian Shufang Cai Min Wang Ruiqiang Li Qi Zhu Xian Tong Ziyun Liang Weilun Jiang Shiyi Chen Yaosheng Chen Zuyong He | 2024 | Journal of Animal Science and Biotechnology2024，15，1： | 0 |
| 4 | Discovery of a first-in-class ANXA3 degrader for the treatment of triple-negative breast cancer显示文摘Triple-negative breast cancer(TNBC)is a nasty disease with extremely high malignancy and poor prognosis.Annexin A3(ANXA3)is a potential prognosis biomarker,displaying an excellent correlation of ANXA3 overexpression with patients'poor prognosis.Silencing the expression of ANXA3effectively inhibits the proliferation and metastasis of TNBC,suggesting that ANXA3 can be a promising therapeutic target to treat TNBC.Herein,we report a first-in-class ANXA3-targeted small molecule(R)-SL18,which demonstrated excellent anti-proliferative and anti-invasive activities to TNBC cells.(R)-SL18 directly bound to ANXA3 and increased its ubiquitination,thereby inducing ANXA3 degradation with moderate family selectivity.Importantly,(R)-SL18 showed a safe and effective therapeutic potency in a high ANXA3-expressing TNBC patient-derived xenograft model.Furthermore,(R)-SL18 could reduce theβ-catenin level,and accordingly inhibit the Wnt/β-catenin signaling pathway in TNBC cells.Collectively,our data suggested that targeting degradation of ANXA3 by(R)-SL18 possesses the potential to treat TNBC. | Yongxi Liang Delin Min Hulin Fan Kunlin Liu Juchuanli Tu Xueyan He Bingjie Liu Lu Zhou Suling Liu Xun Sun | 2023 | Acta Pharmaceutica Sinica B2023，13，4： | 0 |
| 5 | Strategic design of lysine-targeted irreversible covalent NDM-1 inhibitors显示文摘New Delhi metallo-β-lactamase 1(NDM-1) can hydrolyze most β-lactam antibiotics, which is the major factor for drug resistance of Gram-negative bacteria. The binding of most reversible inhibitors to NDM-1 is relatively weak due to the shallow active pocket of NDM-1. Alternatively, irreversible covalent inhibitors can prevent their dissociation from the target, leading to permanent inactivation of the protein.Herein, we report a series of irreversible covalent inhibitors of NDM-1 targeting the conserved Lys211 in the active pocket. Several methods, including mass spectrometry, sodium dodecyl sulfate-polyacrylamide gel electrophoresis, fluorescent labeling, and coumarin probe were used to demonstrate that pentafluorophenyl ester formed a covalent bond with Lys211. Moreover, our target inhibitor, in combination with meropenem, achieved an antibacterial effect on drug-resistant bacteria, along with an excellent safety profile. Our new strategy in designing lysine-targeted irreversible covalent NDM-1 inhibitors provides a potential option for the clinical treatment of Gram-negative bacteria. | Youzhen Ma Yongxi Liang Menglu Guo Delin Min Lulu Zheng Yun Tang Xun Sun | 2023 | Chinese Chemical Letters2023，34，7： | 0 |
您所在的机构暂未开通此部分的功能，请联系管理员