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| 1 | Rice DENSE AND ERECT PANICLE 2 is essential for determining panicle outgrowth and elongation显示文摘包括谷物尺寸和圆锥花序形态学,圆锥花序的建筑学直接决定谷物产量。圆锥花序直立,为在中国的北部分完成理想的植物建筑学被选择,引起了米饭 breeders 的增加的注意。这里,稠密、直立的圆锥花序 2 (dep2 ) 异种,显示出稠密、直立的圆锥花序显型,被识别。没有任何已知的功能的领域, DEP2 编码植物特定的蛋白质。表示介绍 DEP2 表明它高度在年轻纸巾被表示,与在年轻圆锥花序的大多数丰富。词法并且表示分析显示在 DEP2 的那个变化主要影响脊柱和主要、第二等的分支的快速的延伸,但是不损害圆锥花序 primordia 的开始或形成。进一步的分析建议在 dep2 的圆锥花序长度的减少被一个缺点在圆锥花序的指数的延伸期间在房间增长引起。尽管有在 dep2 异种的一种更紧缩的植物类型,在谷物生产的重要改变都没在野类型和 dep2 异种之间被发现。因此, DEP2 的学习不仅加强我们圆锥花序建筑学的分子的基因基础的理解而且为米饭繁殖有重要含意。 | Feng Li Wenbo Liu Jiuyou Tang Jinfeng Chen Hongning Tong Bin Hu Chunlai Li Jun Fang Mingsheng Chen Chengcai Chu | 2010 | Cell Research2010，20，7： | 51 |
| 2 | RLINl,encoding a putative coproporphyrinogen Ⅲ oxidase,is involved in lesion initiation in rice显示文摘Lesion mimic is necrotic lesions on plant leaf or stem in the absence of pathogenic infection,and its exact biological mechanism is varied.By a large-scale screening of our T-DNA mutant population,we identified a mutant rice lesion initiation 1(rlin1),which was controlled by a single nuclear recessive gene.Map-based cloning revealed that RLIN1 encoded a putative coproporphyrinogen Ⅲ oxidase in tetrapyrrole biosynthesis pathway.Sequencing results showed that a G to T substitution occurred in the second exon of RLIN1 and led to a missense mutation from Asp to Tyr.Ectopic expression of RLIN1 could rescue rlin1 lesion mimic phenotype.Histochemical analysis demonstrated that lesion formation in rlin1 was light-dependent accompanied by reactive oxygen species accumulated.These results suggest that tetrapyrrole participates in lesion formation in rice. | Changhui Sun Linchuan Liu Jiuyou Tang Aihong Lin Fantao Zhang Jun Fang Genfa Zhang Chengcai Chu | 2011 | Journal of Genetics and Genomics2011，38，1： | 31 |
| 3 | Degradation of chlorpyrifos in laboratory soil and its impact on soil microbial functional diversity显示文摘Degradation of chlorpyrifos at different concentrations in soil and its impact on soil microbial functional diversity were investigated under laboratory condition.The degradation half-live of chlorpyrifos at levels of 4,8,and 12 mg/kg in soil were calculated to be 14.3,16.7,and 18.0 d,respectively.The Biolog study showed that the average well color development(AWCD) in soils was significantly(P < 0.05) inhibited by chlorpyrifos within the first two weeks and thereafter recovered to a similar level as the control.A similar variation in the diversity indices(Simpson index 1/D and McIntosh index U) was observed,but no significant difference among the values of the Shannon-Wiener index H was found in chlorpyrifos-treated soils.With an increasing chlorpyrifos concentration,the half-life of chlorpyrifos was significantly(P 0.05) extended and its inhibitory effect on soil microorganisms was aggravated.It is concluded that chlorpyrifos residues in soil had a temporary or short-term inhibitory effect on soil microbial functional diversity. | FANG Hua YU Yunlong CHU Xiaoqiang WANG Xiuguo YANG Xiaoe YU Jingquan | 2009 | Journal of Environmental Sciences2009，21，3： | 25 |
| 4 | A novel gene delivery system targeting cells expressing VEGF receptors显示文摘Two ligand oligopeptides GV1 and GV2 were designed according to the putative binding region of VEGF to its receptors. GV1, GV2 and endosome releasing oligopeptide HA20 were conjugated with poly-L-lysine or protamine and the resulting conjugates could interact with DNA in a noncovalent bond to form a complex. Using pSV2-β-galactosidase as a reporter gene, it has been demonstrated that exogenous gene was transferred into bovine aortic arch-derived endothelial cells (ABAE) andhuman malignant melanoma cell lines (A375) in vitro. In vivo experiments, exogenous gene was transferred into tumor vascular endothelial cells and tumor cells of subcutaneously transplanted human colon cancer LOVO, human malignant melanoma A375 and human hepatoma graft in nude mice. This system could also target gene to intrahepatically transplanted human hepatoma injected via portal vein in nude mice. These results are correlated with theGene delivery system targeting VEGF receptors relevant receptors (flt-1, flk-1/KDR) expression on the targeted cells and tissues. | LI JUN MIN JUN SONG HAN YI HUANG PEI KUN TIAN SHU MIN QU MIN YAO HUI QIU JIANG DA FANG WAN JING CHU LUO CHENG XIAO GU JIAN REN GU( National Labomtory for Oncogenes and Related Genes, Shanghai Cancer Institute, Shanghai 200032,China)(National Laboratory of | 1999 | Cell Research1999，9，1： | 22 |
| 5 | Left posterior fascicular block: a new endpoint of ablation for verapamil-sensitive idiopathic ventricular tachycardia显示文摘Background Verapamil-sensitive, idiopathic left ventricular tachycardia (ILVT) with right bundle branch block configuration and left-axis deviation is known to be due to re-entry mechanism but the exact nature of reentrant circuit in ILVT is not fully elucidated. Radiofrequency (RF) ablation was applied during ventricular tachycardia (VT) and termination of the VT or abolishing the inducibility of the tachycardia was used as an endpoint for successful RF. In this study, the left posterior fascicular block in surface electrocardiogram (ECG) was used as a new endpoint of ablation to cure ILVT. Methods Electrophysiological studies and radiofrequency ablation were performed in 39 consecutive patients [30 men, 9 women; age ranging from 10 to 64 years, mean (29±16) years] with verapamil-sensitive ILVT and structurally normal hearts. VT could be terminated by the intravenous administration of verapamil in all patients. The target site was the midseptum of LV where the earliest Purkinje potentials were recorded during VT. RF current was applied to the target site with or without late diastolic potential (LDP) during sinus rhythm in 37 patients and during VT in 2 patients to meet the ablation endpoint: the left posterior fascicular block in the surface ECG. Results Thirty-seven patients with ILVT had been treated by RF ablation during sinus rhythm and two had been treated during VT. All of them met the endpoint of the left posterior fascicular block. Thirty-eight cases were symptom-free without medications during the follow-up period (range from 3 to 95 months, median 17 months). One patient developed a clinical recurrence and the left posterior fascicular block in surface ECG disappeared. The patient received another treatment. The endpoint was met and the procedure was successful. Conclusions The left posterior fascicular block in surface ECG used as an endpoint of RF ablation to treat ILVT is effective. It is important especially in those patients whose VT can not be induced or the inducible condition is unstable. The effective endpoint implied that the left posterior fascicle might be a critical part of the re-entrant circuit. | MA Fu-sheng MA Jian TANG Kai HAN Hao JIAYu-he FANG Pi-hua CHU Jian-min PU Jie-lin ZHANG Shu | 2006 | Chinese Medical Journal2006，，5： | 21 |
| 6 | Climate and litter C/N ratio constrain soil organic carbon accumulation显示文摘Soil organic carbon(SOC) plays critical roles in stabilizing atmospheric CO2 concentration, but the mechanistic controls on the amount and distribution of SOC on global scales are not well understood. In turn, this has hampered the ability to model global C budgets and to find measures to mitigate climate change. Here, based on the data from a large field survey campaign with 2600 plots across China’s forest ecosystems and a global collection of published data from forested land, we find that a low litter carbon-to-nitrogen ratio(C/N) and high wetness index(P/PET, precipitation-to-potentialevapotranspiration ratio) are the two factors that promote SOC accumulation, with only minor contributions of litter quantity and soil texture. The field survey data demonstrated that high plant diversity decreased litter C/N and thus indirectly promoted SOC accumulation by increasing the litter quality. We conclude that any changes in plant-community composition, plant-species richness and environmental factors that can reduce the litter C/N ratio, or climatic changes that increase wetness index, may promote SOC accumulation. The study provides a guideline for modeling the carbon cycle of various ecosystem scales and formulates the principle for land-based actions for mitigating the rising atmospheric CO2 concentration. | Guoyi Zhou Shan Xu Philippe Ciais Stefano Manzoni Jingyun Fang Guirui Yu Xuli Tang Ping Zhou Wantong Wang Junhua Yan Gengxu Wang Keping Ma Shenggong Li Sheng Du Shijie Han Youxin Ma Deqiang Zhang Juxiu Liu Shizhong Liu Guowei Chu Qianmei Zhang Yuelin Li Wenjuan Huang Hai Ren Xiankai Lu Xiuzhi Chen | 2019 | National Science Review2019，6，4： | 21 |
| 7 | High-efficiency breeding of early-maturing rice cultivars via CRISPR/Cas9-mediated genome editing显示文摘Rice is a staple food for more than half of the human population.It has been estimated that by 2030,40%more rice needs to be produced in order to meet the growing demand(Khush,2005).One of the strategies to improve rice productivity is to enlarge rice | Xiufeng Li Wenjia Zhou Yuekun Ren Xiaojie Tian Tianxiao Lv Zhenyu Wang Jun Fang Chengcai Chu Jie Yang Qingyun Bu | 2017 | Journal of Genetics and Genomics2017，44，3： | 20 |
| 8 | Understanding the genetic and epigenetic architecture in complex network of rice flowering pathways显示文摘尽管 flowering 时间控制的分子的基础很好在长天(LD ) 内被把,种 Arabidopsis,它在短天(SD ) 内大部分仍然是未知的植物米饭。瑞斯 flowering 时间(标题日期) 是为季节改编和谷物产量的一个重要农学的特点,它被基因、环境的因素影响。在最后十年期间,作为被识别的、著名进步在探索上被取得了的成花素的性质,成花素基因表示怎么样,遗传上控制。然而,在象 FLOWERING 那样的某些关键 flowering 综合者的 Arabidopsis 表示,地点 C (FLC ) 和 FLOWERING 地点 T (英尺) 被各种各样的染色质修正 epigenetically 也调整几乎不在这个方面上在米饭被知道直到很最近。这评论在米饭 flowering 时间控制总结了基因网络和染色质修正的进展,试图给一个完全的看法基因并且在米饭 flowering 小径的复杂网络的 epigenetic 建筑学。 | Changhui Sun Dan Chen Jun Fang Pingrong Wang Xiaojian Deng Chengcai Chu | 2014 | Protein & Cell2014，5，12： | 19 |
| 9 | Mechanism of zinc damaging to blast furnace tuyere refractory显示文摘The phenomena of tuyere upward-warp have been found at No.6 blast furnace in Kunming Steel Company China after its blow-in,which has made a great impact on the practical production of the furnace.Thus,a number of efforts have been made to elucidate the mechanism of this phenomenon.The results of investigation and tests revealed that the enrichment and expansion of zinc in the tuyere bricks is the main factor leading to the tuyere upward-warp.The eroding behavior of zinc is that the inner structure of the tuyere bricks turns from dense to loose with entering,enriching and expanding of zinc,which forms spot-like→stripe-like→ditch-like→vein-like→tumorlike eroding passage.Additionally,it is found that the sequence of deleterious elements entering the tuyere refractory is K,Na,Zn and Pb,respectively.Finally,the phenomena and process of zinc crystallization and growth in the refractory have been clearly observed and recorded during this investigation. | Xuefeng FANG Mansheng CHU Fengman SHEN Zhuming ZHANG | 2009 | Acta Metallurgica Sinica(English Letters)2009，22，6： | 18 |
| 10 | Caffeic acid ameliorates early and delayed brain injuries after focal cerebral ischemia in rats显示文摘瞄准: 在老鼠,和与 5-lipoxygenase 抑制的可能的关系在焦点的服的局部缺血以后在早、推迟的损害上调查咖啡酸的效果。方法:短暂焦点的服的局部缺血被中间的服的动脉吸藏在 Sprague-Dawley 老鼠导致。咖啡酸(10 和 50 mg/kg ) 是在局部缺血以后为 5 d 注射的 ip。脑损伤被观察,并且 cysteinyl leukotrienes 的层次和在大脑织物的 leukotriene B4 被测量。结果:咖啡酸(50 mg/kg ) 改善了神经病学的机能障碍和神经原损失,和减少的梗塞体积在局部缺血以后的 24 h;它稀释了大脑萎缩,梗塞体积,并且特别地在局部缺血以后的星形细胞增长 14 d。另外,它减少了 leukotrienes (5-lipoxygenase 代谢物) 的生产在是化学家半球在局部缺血以后的 3 h 和 7 d。结论:咖啡酸在老鼠在焦点的服的局部缺血以后在早、推迟的损害上有保护的效果;并且这效果可以部分联系抑制到 5-lipoxygenase。 | Yu ZHOU San-hua FANG Yi-lu YE Li-sheng CHU Wei-ping ZHANG Meng-ling WANG Er-qing WEI | 2006 | Acta Pharmacologica Sinica2006，27，9： | 17 |
| 11 | Mineral and Bone Disorder and Its Association with Cardiovascular Parameters in Chinese Patients with Chronic Kidney Disease显示文摘 | Chu Zhou Fang Wang Jin-Wei Wang Lu-Xia Zhang Ming-Hui Zhao | 2016 | Chinese Medical Journal2016，，19： | 17 |
| 12 | Minocycline inhibits 5-lipoxygenase activation and brain inflammation after focal cerebral ischemia in rats显示文摘目的:决定 minocycline onpostis化学家大脑损害的反煽动性的效果是否被 5-lipoxygenase (5哈鱼)的抑制调停在 rats.Methods 的表示 andenzymatic 激活:焦点的服的局部缺血与 middlecerebral 动脉吸藏为 30 min 被导致,由 reperfusion.The ischemic 损害列在后面,内长的 IgG 渗出, neutrophils 和 macrophage/microglia 的 theaccumulation ,和5哈鱼 mRNA 表示被决定 72 hafter reperfusion.5哈鱼代谢物( | Li-sheng CHU San-hua FANG Yu ZHOU Guo-liang YU Meng-ling WANG Wei-ping ZHANG Er-qing WEI | 2007 | Acta Pharmacologica Sinica2007，28，6： | 16 |
| 13 | Degradation of chlorpyrifos alone and in combination with chlorothalonil and their effects on soil microbial populations显示文摘在实践,杀虫剂通常为庄稼保护同时或一个接一个被使用,并且这类杀虫剂申请经常在土壤环境导致杀虫剂残余的联合污染。实验室研究被进行在土壤上在 chlorpyrifos 降级和它的效果上调查 chlorothalonil 的影响细菌,真菌,并且放射菌类人口。在这里的试验性的条件下面, chlorpyrifos 的一半生活独自,并且在有 chlorothalonil 的联合,在推荐并且两倍剂量,被测量分别地是 3.24, 2.77,和 2.63 d。Chlorpyrifos 降级没被它的联合显著地与 chlorothalonil 改变。然而,土壤微生物上的 chlorpyrifos 的禁止的效果被它的联合与 chlorothalonil 增加,并且增加与增加的 chlorothalonil 的层次有关。在控制,细菌的人口,真菌,和放射菌类比作那些分别地,被 chlorpyrifos 被 44.1% , 61.1% ,和 72.8% 显著地独自在处理(DAT ) 以后在第一天减少。与 chlorothalonil 的增加,抑制在推荐剂量被增加到 55.2% , 79.3% ,和 85.8% ,并且 86.0% , 94.1% ,和 90.8% 在双剂量在一 DAT,分别地。结果建议联合效果应该被考虑估计杀虫剂应用的实际影响。 | CHU Xiaoqiang FANG Hua PAN Xuedong WANG Xiao SHAN Min FENG Bo YU Yunlong | 2008 | Journal of Environmental Sciences2008，20，4： | 16 |
| 14 | The Interactions among DWARF10,Auxin and Cytokinin Underlie Lateral Bud Outgrowth in Rice显示文摘Previous studies have shown that DWARF10(D10) is a rice ortholog of MAX4/RMS1/DAD1,encoding a carotenoid cleavage dioxygenase and functioning in strigolactones/strigolactone-derivatives(SL) biosynthesis.Here we use D10-RNA interference(RNAi) transgenic plants similar to d10 mutant in phenotypes to investigate the interactions among D10,auxin and cytokinin in regulating rice shoot branching.Auxin levels in node 1 of both decapitated D10-RNAi and wild type plants decreased significantly,showing that decapitation does reduce endogenous auxin concentration,but decapitation has no clear effects on auxin levels in node 2 of the same plants.This implies that node 1 may be the location where a possible interaction between auxin and D10 gene would be detected.D10 expression in node 1 is inhibited by decapitation,and this inhibition can be restored by exogenous auxin application,indicating that D10 may play an important role in auxin regulation of SL.The decreased expression of most OsPINs in shoot nodes of D10-RNAi plants may cause a reduced auxin transport capacity.Furthermore,effects of auxin treatment of decapitated plants on the expression of cytokinin biosynthetic genes suggest that D10 promotes cytokinin biosynthesis by reducing auxin levels.Besides,in D10-RNAi plants,decreased storage cytokinin levels in the shoot node may partly account for the increased active cytokinin contents,resulting in more tillering phenotypes. | Shuying Zhang Gang Li Jun Fang Weiqi Chen Haipai Jiang Junhuang Zou Xue Liu Xianfeng Zhao Xiaobing Li Chengcai Chu Qi Xie Xiangning Jiang Lihuang Zhu | 2010 | Journal of Integrative Plant Biology2010，52，7： | 14 |
| 15 | Preparation, characterization and salt-resistance of a coal based super absorbent composite显示文摘Potassium humate was extracted from brown coal. A novel super absorbent composite, poly (acrylic acid-co-acryla-mide)/potassium humate (PAA-AM/KHA), was prepared by graft polymerization of acrylic acid, acrylamide and coal based potas-sium humate using N, N’-methylenebisacrylamide as a crosslinker and potassium peroxydisulfate as an initiator. The effects of reaction temperature, degree of neutralization of the poly (acrylic acid) and the amounts of crosslinker, initiator and potassium hu-mate were investigated. Salt resistance tests were also carried out. The composite prepared under optimal conditions had a potas-sium humate content of 10% and exhibited a water absorption of 770 g/g in distilled water, and 349, 286 and 41 g/g in 0.5 mol/L KCl, MgCl2 and AlCl3 solutions respectively. The results indicate that the salt resistance of PAA-AM/KHA was superior to that of poly (acrylic acid-co-acrylamide) because of the collaborative effect of functional groups of the coal based potassium humate. The PAA-AM/KHA micro powder was characterized by IR spectroscopy and the micrographic surface was characterized by scanning electron microscopy. Introduction of potassium humate into the poly (acrylic acid-co-acrylamide) structure creates a composite more suitable for use as a water-managing material in the renewal of arid and desert environments. The salt resisting property of the composite is improved, production costs are reduced and the growth stimulant effect is still present. | CHU Mo, HUANG Zhanbin, XU Bang, FANG Tao, DUAN Hui School of Chemical and Environmental Engineering, China University of Mining & Technology, Beijing 100083, China | 2010 | Mining Science and Technology2010，20，6： | 13 |
| 16 | Jiao-tai-wan Up-regulates Hypothalamic and Peripheral Circadian Clock Gene Cryptochrome and Activates PI3K/AKT Signaling in Partially Sleep-deprived Rats显示文摘 | Wen-ya HUANG Xin ZOU Fu-er LU Hao SU Chu ZHANG Yan-lin REN Ke FANG Li-jun XU Kai- fu WANG Qing-jie CHEN Hui DONG | 2018 | Current Medical Science2018，38，4： | 13 |
| 17 | Paleoenvironmental records from the northern South China Sea since the Last Glacial Maximum显示文摘Core ZHS-176 contains the paleoenvironmental records from the northern South China Sea (NSCS) since the Last Glacial Maximum (LGM). A coupled approach based on clay mineral assemblages, planktonic foraminiferal oxygen and carbon isotopes, and calcium carbonate content is used to trace the sources of the fine-grained sediment and to investigate the paleoenviornmental evolution in this area. Clay mineral assemblages are dominated by illite (average about 39%) and chlorite (about 27%), which comes mainly from Taiwan and the East China Sea. Kaolinite, which accounts for about 13%, comes mainly from the Zhujiang (Pearl) River, and Luzon Island is the main source for smectite (about 21%). The planktonic foraminiferal oxygen isotopic oscillations during the last glacial period are coeval with climate variations recorded in the Greenland ice core and Western Pacific sediment. These variations include the LGM, Heinrich event 1, Bφlling-Allerφd (B/A), and Younger Dryas. For the Holocene, three periods of strong precipitation (S1-S3) and three periods of weak precipitation (W1-W3) are identified. The oxygen isotopic record exhibits corre-lation with climate records from distant regions, including the high-latitude Northern Hemisphere, providing evidence for global tele-connection among regional climate. A brief, negative planktonic foraminiferal carbon isotopic excursion during B/A reflects increased methane released from marine gas hydrate due to the rapid warming of the water. By comparing calcium carbonate content curves of the core ZHS-176 with these of other five boreholes lying above the lysocline, a remarkable low calcium carbonate event is found during the early Holocene in NSCS. | GE Qian CHU Fengyou XUE Zuo LIU J Paul DU Yuansheng FANG Yinxia | 2010 | Acta Oceanologica Sinica2010，29，3： | 13 |
| 18 | Combinations of Hd2 and Hd4 genes determine rice adaptability to Heilongjiang Province, northern limit of China显示文摘Heading date is a key trait in rice domestication and adaption, and a number of quantitative trait loci(QTLs)have been identified. The rice(Oryza sativa L.) cultivars in the Heilongjiang Province, the northernmost region of China,have to flower extremely early to fulfill their life cycle.However, the critical genes or different gene combinations controlling early flowering in this region have not been determined. QTL and candidate gene analysis revealed that Hd2/Ghd7.1/Os PRR37 plays a major role in controlling rice distribution in Heilongjiang. Further association analysis with a collection of rice cultivars demonstrated that another three major QTL genes(Hd4/Ghd7, Hd5/DTH8/Ghd8, and Hd1)also participate in regulating heading date under natural long day(LD) conditions. Hd2/Ghd7.1/Os PRR37 and Hd4/Ghd7 are two major QTLs and function additively. With the northward rice cultivation, the Hd2/Ghd7.1/Os PRR37 and Hd4/Ghd7 haplotypes became non-functional alleles. Hd1 might be non-functional in most Heilongjiang rice varieties,implying that recessive hd1 were selected during local rice breeding. Non-functional Hd5/DTH8/Ghd8 is very rare, but constitutes a potential target for breeding extremely early flowering cultivars. Our results indicated that diverse genetic combinations of Hd1, Hd2, Hd4, and Hd5 determined the different distribution of rice varieties in this northernmost province of China. | Xiufeng Li Huazhao Liu Maoqing Wang Hualong Liu Xiaojie Tian Wenjia Zhou Tianxiao Lü Zhenyu Wang Chengcai Chu Jun Fang Qingyun Bu | 2015 | Journal of Integrative Plant Biology2015，57，8： | 13 |
| 19 | ζ-Carotene Isomerase Suppresses Tillering in Rice through the Coordinated Biosynthesis of Strigolactone and Abscisic Acid显示文摘Rice tillering is an important agronomic trait affecting grain yield.Here,we identified a high-tillering mutant tillering20(t20),which could be restored to the wild type by treatment with the strigolactone(SL)analog rac-GR24.T20 encodes a chloroplast ζ-carotene isomerase(Z-ISO),which is involved in the biosynthesis of ca-rotenoids and their metabolites,SL and abscisic acid(ABA).The t20 mutant has reduced SL and ABA,raising the question of how SL and ABA biosynthesis is coordinated,and whether they have overlapping functions in tillering.We discovered that rac-GR24 stimulated T20 expression and enhanced all-trans-p-carotene biosynthesis.Importantly,rac-GR24 also stimulated expression of Oryza sativa 9-CIS-EPOXY-CAROTENOID DIOXYGENASE 1(OsNCED1)through induction of Oryza sativa HOMEOBOX12(0sHOX12),promoting ABA biosynthesis in shoot base.On the other hand,ABA treatment significantly repressed SL biosynthesis and the ABA biosynthetic mutants displayed elevated SL biosynthesis.ABA treatment reduced the number of basal tillers in both t20 and wild-type plants.Furthermore,while ABA-deficient mu-tants aba1 and aba2 had the same number of basal tillers as wild type,they had more unproductive upper tillers at maturity.This work demonstrates complex interactions in the biosynthesis of carotenoid,SLs and ABA,and reveals a role for ABA in the regulation of rice tillering. | Xue Liu Qingliang Hu Jijun Yan Kai Sun Yan Liang Meiru Jia Xiangbing Meng Shuang Fang Yiqin Wang Yanhui Jing Guifu Liu Dianxing Wu Chengcai Chu Steven M.Smith Jinfang Chu Yonghong Wang Jiayang Li Bing Wang | 2020 | Molecular Plant2020，13，12： | 11 |
| 20 | Generation of a Hutchinson-Gilford progeria syndrome monkey model by base editing显示文摘Many human genetic diseases,including Hutchinson-Gilford progeria syndrome(HGPS),are caused by single point mutations.HGPS is a rare disorder that causes premature aging and is usually caused by a de novo point mutation in the LMNA gene.Base editors(BEs)composed of a cytidine deaminase fused to CRISPR/Cas9 nickase are highly efficient at inducing C to T base conversions in a programmable manner and can be used to generate animal disease models with single amino-acid substitutions.Here,we generated the first HGPS monkey model by delivering a BE mRNA and guide RNA(gRNA)targeting the LMNA gene via microinjection into monkey zygotes.Five out of six newborn monkeys carried the mutation specifically at the target site.HGPS monkeys expressed the toxic form of lamin A,progerin,and recapitulated the typical HGPS phenotypes including growth retardation,bone alterations,and vascular abnormalities.Thus,this monkey model genetically and clinically mimics HGPS in humans,demonstrating that the BE system can efficiently and accurately generate patient-specific disease models in non-human primates. | Fang Wang Weiqi Zhang Qiaoyan Yang Yu Kang Yanling Fan Jingkuan Wei Zunpeng Liu Shaoxing Dai Hao Li Zifan Li Lizhu Xu Chu Chu Jing Qu Chenyang Si Weizhi Ji Guang-Hui Liu Chengzu Long Yuyu Niu | 2020 | Protein & Cell2020，11，11： | 11 |
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