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| 1 | IPA1 functions as a downstream transcription factor repressed by D53 in strigolactone signaling in rice显示文摘Strigolactones (SL ) ,一组类胡萝卜素导出 terpenoid 内酯,是压制射击由禁止腋的芽的长出分叉的 root-to-shoot 植物激素。矮子 53 (D53 ) ,表明小径的 SL 的关键抑压者,被推测调整 SL 反应的下游的 transcriptional 网络。然而, D53 指向的下游的抄写因素还都没被报导。这里,我们报导那理想的植物体系结构 1 (IPA1 ) ,在米饭的植物体系结构的一个关键管理者,在调整 tiller 数字和导致 SL 的基因表示作为 D53 的一个直接下游的部件工作。我们证明 D53 在 vivo 并且在 vitro 与 IPA1 交往并且压制 IPA1 的 transcriptional 激活活动。我们进一步证明 IPA1 能直接绑在 D53 倡导者并且在导致 SL 的 D53 表示的反馈规定起一个关键作用。这些调查结果表明 IPA1 是可能的与 D53 行动到的长推测的抄写因素之一调停在米饭的调整 SL 的 tiller 发展。 | Xiaoguang Song Zefu Lu Hong Yu Gaoneng Shao Jinsong Xiong Xiangbing Meng Yanhui Jing Guifu Liu Guosheng Xiong Jingbo Duan Xue-Feng Yao Chun-Ming Liu Hongqing Li Yonghong Wang Jiayang Li | 2017 | Cell Research2017，27，9： | 48 |
| 2 | Construction of a Genome-Wide Mutant Library in Rice Using CRISPR/Cas9显示文摘 | Meng, Xiangbing Yu, Hong Zhang, Yi Zhuang, Fengfeng Song, Xiaoguang Gao, Songsong Gao, Caixia Li, Jiayang | 2017 | Molecular Plant2017，10，9： | 46 |
| 3 | A Strigolactone Biosynthesis Gene Contributed to the Green Revolution in Rice显示文摘Plant architecture is a complex agronomic trait and a major factor of crop yield,which is affected by several important hormones.Strigolactones(SLs)are identified as a new class hormoneinhibiting branching in many plant species and have been shown to be involved in various developmental processes.Genetical and chemical modulation of the SL pathway is recognized as a promising approach to modify plant architecture.However,whether and how the genes involved in the SL pathway could be utilized in breeding still remain elusive.Here,we demonstrate that a partial loss-of-function allele of the SL biosynthesis gene,HIGH TILLERING AND DWARF 1/DWARF17(HTD1/D17),which encodes CAROTENOID CLEAVAGE DIOXYGENASE 7(CCD7),increases tiller number and improves grain yield in rice.We found that the HTD1 gene had been widely utilized and co-selected with Semidwarf 1(SD1),both contributing to the improvement of plant architecture in modern rice varieties since the Green Revolution in the 1960s.Understanding how phytohormone pathway genes regulate plant architecture and how they have been utilized and selected in breeding will lay the foundation for developing the rational approaches toward improving crop yield. | Yuexing Wang Lianguang Shang Hong Yu Longjun Zeng Jiang Hu Shen Ni Yuchun Rao Sanfeng Li Jinfang Chu Xiangbing Meng Lei Wang Ping Hu Jijun Yan Shujing Kang Minghao Qu Hai Lin Tao Wang Quan Wang Xingming Hu Hongqi Chen Bing Wang Zhenyu Gao Longbiao Guo Dali Zeng Xudong Zhu Guosheng Xiong Jiayang Li Qian Qian | 2020 | Molecular Plant2020，13，6： | 23 |
| 4 | MONOCULM 3,an Ortholog of WUSCHEL in Rice,Is Required for Tiller Bud Formation显示文摘WUSCHEL(WUS)plays an essential role for the maintenance of meristem activity in dicots,but its function is still elusive in monocots.We isolated a new monoculm mutant,monoculm 3(moc3),in which a point mutation causes the premature termination of rice O.sativa WUS(Os WUS).Morphological observation revealed that the formation of tiller buds was disrupted in moc3.MOC3 was localized in the nuclear and could interact with TOPLESS-RELATED PROTEINS(TPRs).The expression of MOC3 was induced by cytokinins and defection of MOC3 affected the expression of several two-component cytokinin response regulators,Os RRs and ORRs.Our results suggest that MOC3 is required for the formation of axillary buds and has a complex relationship with cytokinins. | Zefu Lu Gaoneng Shao Jinsong Xiong Yongqing Jiao Jing Wang Guifu Liu Xiangbing Meng Yan Liang Guosheng Xiong Yonghong Wang Jiayang Li | 2015 | Journal of Genetics and Genomics2015，42，2： | 17 |
| 5 | Tiller Bud Formation Regulators MOC1 and MOC3 Cooperatively Promote Tiller Bud Outgrowth by Activating F0N1 Expression in Rice显示文摘Tillering in rice is one of the most important agronomic traits.Rice tiller development can be divided into two main processes: the formation of the axillary bud and its subsequent outgrowth.Several genes critical for bud formation in rice have been identified by genetic studies;however,their molecular functions and relationships are still largely unknown.Here,we report that MONOCULM 1 (MOC1) and MONOCULM 3/ TILLERS ABSENT 1/STERILE AND REDUCED TILLERING 1 (MOC3/TAB1/SRT1),two vital regulators for tiller formation in rice,physically interact to regulate tiller bud outgrowth through upregulating the expression of FLORAL ORGAN NUMBER 1 (FON1),the homolog of CLAVATA1 in rice.We found that M0C3 is able to directly bind the promoter ofFONI and subsequently activate FON1 expression.MOC1 functions as a coactivator of MOC3,whereas it could not directly bind the FON1 promoter,and further activated FON1 expression in the presence of MOC3.Accordingly,FON1 is highly expressed at axillary meristems and shows remarkably decreased expression levels in mod and moc3 mutants.Loss-of-function mutants of FON1 exhibit normal bud formation but defective bud outgrowth and reduced tiller number.Collectively,these results shed light on a joint transcriptional regulatory mechanim by MOC1 and MOC3,and establish a new framework for the control of tiller bud formation and outgrowth. | Gaoneng Shao Zefu Lu Jinsong Xiong Bing Wang Yanhui Jing Xiangbing Meng Guifu Liu Haiyan Ma Yan Liang Fan Chen Yonghong Wang Jiayang Li Hong Yu | 2019 | Molecular Plant2019，12，8： | 15 |
| 6 | OsBRXL4 Regulates Shoot Gravitropism and Rice Tiller Angle through Affecting LAZY1 Nuclear Localization显示文摘Rice tiller angle is a key agronomic trait that contributes to ideal plant architecture and grain production.LAZY1 (LA1) was previously shown to control tiller angle via affecting shoot gravitropism,but the underlying molecular mechanism remains largely unknown.In this study,we identified an LA1-interacting protein named Brevis Radix Like 4 (OsBRXL4).We showed that the interaction between OsBRXL4 and LA1 occurs at the plasma membrane and that their interaction determines nuclear localization of LA1.We found that nuclear localization of LA1 is essential for its function,which is different from AtLA1,its Arabidopsis ortho.log.Overexpression of OsBRXL4 leads to a prostrate growth phenotype,whereas OsBRXLs RNAi plants,in which the expression levels of OsBRXLI,OsBRXL4,and OsBRXL5 were decreased,display a compact phenotype.Further genetic analysis also supported that OsBRXL4 controls rice tiller angle by affecting nuclear localization of LA1.Consistently,we demonstrated that OsBRXL4 regulates the shoot gravitropism through affecting polar auxin transport as did LA1.Taken together,our study not only identifies OsBRXL4 as a regulatory component of rice tiller angle but also provides new insights into genetic regulation of rice plant architecture. | Zhen Li Yan Liang Yundong Yuan Lei Wang Xiangbing Meng Guosheng Xiong Jie Zhou Yueyue Cai Ningpei Han Lekai Hua Guifu Liu Jiayang Li Yonghong Wang | 2019 | Molecular Plant2019，12，8： | 12 |
| 7 | Robust genome editing of CRISPR-Cas9 at NAG PAMs in rice显示文摘Dear Editor,The CRISPR-Cas9(clustered regularly interspaced short palindromic repeats/Cas9)system has been widely used for a variety of applications,including targeted gene knockout,gene insertion,gene replacement and base editing.Despite its wide use,the genome editing using CRISPR-Cas9 is performed almost exclusively at sites containing canonical NGG protospacer adjacent motifs(PAMs).To overcome the PAM constraint of the CRISPR-Cas9 system,many attempts have been made to develop various Cas9 orthologs | Xiangbing Meng Xixun Hu Qing Liu Xiaoguang Song Caixia Gao Jiayang Li Kejian Wang | 2018 | Science China(Life Sciences)2018，61，1： | 11 |
| 8 | ζ-Carotene Isomerase Suppresses Tillering in Rice through the Coordinated Biosynthesis of Strigolactone and Abscisic Acid显示文摘Rice tillering is an important agronomic trait affecting grain yield.Here,we identified a high-tillering mutant tillering20(t20),which could be restored to the wild type by treatment with the strigolactone(SL)analog rac-GR24.T20 encodes a chloroplast ζ-carotene isomerase(Z-ISO),which is involved in the biosynthesis of ca-rotenoids and their metabolites,SL and abscisic acid(ABA).The t20 mutant has reduced SL and ABA,raising the question of how SL and ABA biosynthesis is coordinated,and whether they have overlapping functions in tillering.We discovered that rac-GR24 stimulated T20 expression and enhanced all-trans-p-carotene biosynthesis.Importantly,rac-GR24 also stimulated expression of Oryza sativa 9-CIS-EPOXY-CAROTENOID DIOXYGENASE 1(OsNCED1)through induction of Oryza sativa HOMEOBOX12(0sHOX12),promoting ABA biosynthesis in shoot base.On the other hand,ABA treatment significantly repressed SL biosynthesis and the ABA biosynthetic mutants displayed elevated SL biosynthesis.ABA treatment reduced the number of basal tillers in both t20 and wild-type plants.Furthermore,while ABA-deficient mu-tants aba1 and aba2 had the same number of basal tillers as wild type,they had more unproductive upper tillers at maturity.This work demonstrates complex interactions in the biosynthesis of carotenoid,SLs and ABA,and reveals a role for ABA in the regulation of rice tillering. | Xue Liu Qingliang Hu Jijun Yan Kai Sun Yan Liang Meiru Jia Xiangbing Meng Shuang Fang Yiqin Wang Yanhui Jing Guifu Liu Dianxing Wu Chengcai Chu Steven M.Smith Jinfang Chu Yonghong Wang Jiayang Li Bing Wang | 2020 | Molecular Plant2020，13，12： | 11 |
| 9 | Regulation of Rice Tillering by RNA-Directed DNA Methylation at Miniature Inverted-Repeat Transposable Elements显示文摘Tillering is a major determinant of rice plant architecture and grain yield.Here,we report that depletion of rice OsNRPD1a and OsNRPD1b,two orthologs of the largest subunit of RNA polymerase IV,leads to a high-tillering phenotype,in addition to dwarfism and smaller panicles.OsNRPD1a and OsNRPD1b are required for the production of 24-nt small interfering RNAs that direct DNA methylation at transposable elements(TEs)including miniature inverted-repeat TEs(MITEs).Interestingly,many genes are regulated either positively or negatively by TE methylation.Among them,OsMIR156d and OsMIR156j,which promote rice tillering,are repressed by CHH methylation at two MITEs in the promoters.By contrast,D14,which suppresses rice tillering,is activated by CHH methylation at an MITE in its downstream.Our findings reveal regulation of rice tillering by RNA-directed DNA methylation at MITEs and provide potential targets for agronomic trait enhancement through epigenome editing. | Le Xu Kun Yuan Meng Yuan Xiangbing Meng Min Chen Jianguo Wu Jiayang Li Yijun Qi | 2020 | Molecular Plant2020，13，6： | 9 |
| 10 | FED:a web tool for foreign element detection of genome-edited organism显示文摘Dear Editor,Genome editing,especially the newly developed CRISPR technology,is now widely implemented for diverse medical and agricultural applications(Puchta,2018).However,for genome editing,the DNA cassettes encoding the editing components are usually assembled and delivered into the cells of organisms(Cong et al.,2013). | Qing Liu Xiaozhen Jiao Xiangbing Meng Chun Wang Cao Xu Zhixi Tian Chuanxiao Xie Genying Li Jiayang Li Hong Yu Kejian Wang | 2021 | Science China(Life Sciences)2021，64，1： | 6 |
| 11 | Generating broad-spectrum tolerance to ALS-inhibiting herbicides in rice by base editing显示文摘Herbicide-tolerant rice varieties generated by genome editing are highly desirable for weed control.We have used a cytosine base editor to create a series of missense mutations in the P171 and/or G628 codons of the acetolactate synthase(ALS)gene to confer herbicide tolerance in rice.The four different missense mutations in the P171 codon,P171S,P171A,P171Yand P171F,exhibited different patterns of tolerance towards five representative herbicides from five chemical families of ALS inhibitors.For example,P171S and P171A had lower levels of tolerance than P171Y and P171F to bispyribac but not to the other herbicides.Interestingly,a novel triple mutant(P171F/G628E/G629S)had the highest tolerance to all five tested herbicides.Field trials showed that both P171F and P171F/G628E/G629S could potentially be used with nicosulfuron.Our work illustrates an effective way of using base editing to generate herbicide tolerance in elite rice varieties. | Rui Zhang Sha Chen Xiangbing Meng Zhuangzhuang Chai Delin Wang Yuge Yuan Kunling Chen Linjian Jiang Jiayang Li Caixia Gao | 2021 | Science China(Life Sciences)2021，64，10： | 4 |
| 12 | ScCas9 recognizes NNG protospacer adjacent motif in genome editing of rice显示文摘The CRISPR/Cas(clustered regularly in terspaced short palindromic repeats/CRISPR-associated protein)system has been widely used in genome editing,epigenetic modification,and other applications,because it is a precise,inexpensive,powerful and easy-to?use editing tool(Zhang et al.,2019).However,the scope of genome editing is limited by a short protospacer adjacent motif(PAM)specific sequence flanking the target site.Streptococcus pyogenes Cas9(5^Cas9),the most widely used Cas,strongly recognizes NGG PAM and partially recognizes NAG PAM in rice and is restricted to target the sites with these motifs(Anders et al.,2014;Meng et al.,2018). | Yibo Xu Xiangbing Meng Junjie Wang Baoxiang Qin Kejian Wang Jiayang Li Chun Wang Hong Yu | 2020 | Science China(Life Sciences)2020，63，3： | 4 |
| 13 | Expanding the scope of genome editing with SpG and SpRY variants in rice显示文摘Dear Editor,The clustered regularly interspaced short palindromic repeats/CRISPR-associated nuclease 9(CRISPR/Cas9)system,since it was excavated,has been rapidly developed and sparked a revolution in the genome editing field.In principle,CRISPR/Cas9 system relies on the recognition of specific loci on the genome,which is titled the protospacer adjacent motif(PAM).However,the canonical Streptococcus pyogenes Cas9(SpCas9)nuclease only recognizes NGG or NAG PAMs,rendering an inherent obstacle in amplifying the application of CRISPR/Cas9 technology. | Jun Ren Xiangbing Meng Fengyue Hu Qing Liu Yuexuan Cao Huiying Li Changjie Yan Jiayang Li Kejian Wang Hong Yu Chun Wang | 2021 | Science China(Life Sciences)2021，64，10： | 4 |
| 14 | Improving the efficiency of prime editing with epegRNAs and high-temperature treatment in rice显示文摘Dear Editor,Prime editing(PE)systems are important genome-editing tools developed for mediating arbitrary small DNA insertions,deletions and all 12 base-to-base conversions using a CRISPR-nCas9 fusion of an engineered M-MLV and PE guide RNAs(peg RNAs)(Anzalone et al.,2019). | Jinpeng Zou Xiangbing Meng Qing Liu Meiqi Shang Kejian Wang Jiayang Li Hong Yu Chun Wang | 2022 | Science China(Life Sciences)2022，65，11： | 3 |
| 15 | Enhancing rice grain production by manipulating the naturally evolved cis-regulatory element-containing inverted repeat sequence of OsREM20显示文摘Grain number per panicle(GNP)is an important agronomic trait that contributes to rice grain yield.Despite its importance in rice breeding,the molecular mechanism underlying GNP regulation remains largely unknown.In this study,we identified a previously unrecognized regulatory gene that controls GNP in rice,Oryza sativa REPRODUCTIVE MERISTEM 20(OsREM20),which encodes a B3 domain transcription factor.Through genetic analysis and transgenic validation we found that genetic variation in the CArG box-containing inverted repeat(IR)sequence of the OsREM20 promoter alters its expression level and contributes to GNP variation among rice varieties.Furthermore,we revealed that the IR sequence regulates OsREM20 expression by affecting the direct binding of OsMADS34 to the CArG box within the IR sequence.Interestingly,the divergent pOsREM20IR and pOsREM20ΔIR alleles were found to originate from different Oryza rufipogon accessions,and were independently inherited into the japonica and indica subspecies,respectively,during domestication.Importantly,we demonstrated that IR sequence variations in the OsREM20 promoter can be utilized for germplasm improvement through either genome editing or traditional breeding.Taken together,our study characterizes novel genetic variations responsible for GNP diversity in rice,reveals the underlying molecular mechanism in the regulation of agronomically important gene expression,and provides a promising strategy for improving rice production by manipulating the cis-regulatory element-containing IR sequence. | Xiaowei Wu Yan Liang Hengbin Gao Jiyao Wang Yan Zhao Lekai Hua Yundong Yuan Ahong Wang Xiaohui Zhang Jiafan Liu Jie Zhou Xiangbing Meng Dahan Zhang Shaoyang Lin Xuehui Huang Bin Han Jiayang Li Yonghong Wang | 2021 | Molecular Plant2021，14，6： | 3 |
| 16 | OsMPK4 promotes phosphorylation and degradation of IPA1 in response to salt stress to confer salt tolerance in rice显示文摘Salt stress adversely affects plant growth,development,and crop yield.Rice(Oryza sativa L.)is one of the most salt-sensitive cereal crops,especially at the early seedling stage.Mitogen-activated protein kinase(MAPK/MPK)cascades have been shown to play critical roles in salt response in Arabidopsis.However,the roles of the MPK cascade signaling in rice salt response and substrates of Os MPK remain largely unknown.Here,we report that the salt-induced Os MPK4-Ideal Plant Architecture 1(IPA1)signaling pathway regulates the salt tolerance in rice.Under salt stress,Os MPK4 could interact with IPA1 and phosphorylate IPA1 at Thr180,leading to degradation of IPA1.Genetic evidence shows that IPA1 is a negative regulator of salt tolerance in rice,whereas Os MPK4 promotes salt response in an IPA1-dependent manner.Taken together,our results uncover an Os MPK4-IPA1 signal cascade that modulates the salt stress response in rice and sheds new light on the breeding of salt-tolerant rice varieties. | Meiru Jia Nan Luo Xiangbing Meng Xiaoguang Song Yanhui Jing Liquan Kou Guifu Liu Xiahe Huang Yingchun Wang Jiayang Li Bing Wang Hong Yu | 2022 | Journal of Genetics and Genomics2022，49，8： | 2 |
| 17 | Low phosphorus promotes NSP1–NSP2 heterodimerization to enhance strigolactone biosynthesis and regulate shoot and root architecture in rice显示文摘Phosphorus is an essential macronutrient for plant development and metabolism,and plants have evolved ingenious mechanisms to overcome phosphate(Pi)starvation.However,the molecular mechanisms underlying the regulation of shoot and root architecture by low phosphorus conditions and the coordinated utilization of Pi and nitrogen remain largely unclear.Here,we show that Nodulation Signaling Pathway 1(NSP1)and NSP2 regulate rice tiller number by promoting the biosynthesis of strigolactones(SLs),a class of phytohormones with fundamental effects on plant architecture and environmental responses.We found that NSP1 and NSP2 are induced by Oryza sativa PHOSPHATE STARVATION RESPONSE2(OsPHR2)in response to low-Pi stress and form a complex to directly bind the promoters of SL biosynthesis genes,thus markedly increasing SL biosynthesis in rice.Interestingly,the NSP1/2–SL signaling module represses the expression of CROWN ROOTLESS 1(CRL1),a newly identified early SL-responsive gene in roots,to restrain lateral root density under Pi deficiency.We also demonstrated that GR24^(4DO) treatment under normal conditions inhibits the expression of OsNRTs and OsAMTs to suppress nitrogen absorption but enhances the expression of OsPTs to promote Pi absorption,thus facilitating the balance between nitrogen and phosphorus uptake in rice.Importantly,we found that NSP1p:NSP1 and NSP2p:NSP2 transgenic plants show improved agronomic traits and grain yield under low-and medium-phosphorus conditions.Taken together,these results revealed a novel regulatory mechanism of SL biosynthesis and signaling in response to Pi starvation,providing genetic resources for improving plant architecture and nutrient-use efficiency in low-Pi environments. | Kun Yuan Hao Zhang Chaoji Yu Nan Luo Jijun Yan Shuang Zheng Qingliang Hu Dahan Zhang Liquan Kou Xiangbing Meng Yanhui Jing Mingjiang Chen Xinwei Ban Zongyun Yan Zefu Lu Jian Wu Yu Zhao Yan Liang Yonghong Wang Guosheng Xiong Jinfang Chu Ertao Wang Jiayang Li Bing Wang | 2023 | Molecular Plant2023，16，11： | 2 |
| 18 | Rice gene OsNAC19 encodes a novel NAC-domain transcription factor and responds to infection by Magnaporthe grisea显示文摘 | Ruiming Lin Wensheng Zhao Xiangbing Meng Min Wang Youliang Peng | 2006 | Plant Science2006，，1： | 1 |
| 19 | An engineered platform for reconstituting functional multisubunit SCF E3 ligase in vitro显示文摘Multisubunit SKP1/Cullin1/F-box(SCF)E3 ligases play essential roles in regulating the stability of crucial regulatory factors and controlling growth and development in eukaryotes.Detecting E3 ligase activity in vitro is important forexploring the molecular mechanism of protein ubiquitination.However,in vitro ubiquitination assay systems for multisubunit E3 ligases remain difficult to achieve,especially in plants,mainly owing to difficulties in achieving active components of multisubunit E3 ligases with high purity and characterizing specific E2 and E3 pairs.In this study,we characterized components of the rice ScFDiwARF3(SCFDs)E3 ligase,screened the coordinated E2,and reconstituted active ScFD3 E3 ligase in vitro.We further engineered SCFD3 E3 ligase using a fused SKP1-Cullin1-RBX1(eSCR)protein and found that both the wild-type SCFD3 E3 ligase and the engineered SCFD3 E3 ligase catalyzed ubiquitination of the substrate D53,which is the key transcriptional repressor in strigolactone signaling.Finally,we replaced D3 with other F-box proteins from rice and humans and reconstituted active escF E3 ligases,including escFaID2,escFBxL1s,and escFcDC4 E3 ligases.Our work reconstitutes functional SCF E3 ligases in vitro and generates an engineered system with interchangeable F-box proteins,providing a powerful platform for studying the mechanisms of multisubunit SCF E3 ligases in eukaryotes. | Huihui Liu Simiao Liu Hong Yu Xiahe Huang Yingchun Wang Liang Jiang Xiangbing Meng Guifu Liu Mingjiang Chen Yanhui Jing Feifei Yui Bing Wang Jiayang Li | 2022 | Molecular Plant2022，15，8： | 1 |
| 20 | Cloning and characterization of the DHDPS gene encoding the lysine biosyn- thetic enzyme dihydrodipocolinate synthase from Zizania latifolia (Griseb) 显示文摘 | Kong Fanna Jiang Shaomei Meng Xiangbing | 2009 | Plant Mol Biol Rep2009，27，2： | 1 |
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