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| 1 | Bedaquiline-containing regimens in patients with pulmonary multidrug-resistant tuberculosis in China:focus on the safety显示文摘Background:World Health Organization recommends countries introducing new drug and short treatment regimen for drug resistant tuberculosis(DR-TB)should develop and implement a system for active pharmacovigilance that allows for detection,reporting and management of adverse events.The aim of the study is to evaluate the frequency and severity of adverse events(AEs)of bedaquiline-containing regimen in a cohort of Chinese patients with multidrug-resistant(MDR)/extensively drug-resistant(XDR)-TB based on active drug safety monitoring(aDSM)system of New Drug Introduction and Protection Program(NDIP). | Jing-Tao Gao Juan Du Gui-Hui Wu Yi Pei Meng-Qiu Gao Leonardo Martinez Lin Fan Wei Chen Li Xie Yu Chen Hua Wang Long Jin Guo-Bao Li Pei-Lan Zong Yu Xiong Qian-Hong Wu Ming-Wu Li Xiao-Feng Yan Yan-Fang Miao Qing-Shan Cai Xin-Jie Li Da-Peng Bai Shu-Jun Geng Guo-Li Yang Pei-JunTang Yi Zeng Xiao-Hong Chen Tong-Xia Li Cui Cai Yun Zhou Ma Zhuo Jian-Yun Wang Wen-Long Guan Lin Xu Ji-Chan Shi Wei Shu Li-Li Cheng FeiTeng Yu-Jia Ning Shi-Heng Xie Yu-Xian Sun Li-Jie Zhang Yu-Hong Liu | 2021 | Infectious Diseases of Poverty2021，10，2： | 11 |
| 2 | Modified citrus pectin inhibited bladder tumor growth through downregulation of galectin-3显示文摘Modiled citrus pectin (MCP) is a carbohydrate enriched complex, which has been implicated in cancer treatment and prevention. However, the effects of MCP on urinary bladder cancer (UBC) are unknown. In this study, MCP was first tested in T24 and J82 human UBC cells and showed the inhibition of cell viability by the sulforhodamine B (SRB) assay. The MCP-treated UBC cells exhibited G2/M phase arrest with the decrease of Cyclin B1 and phosphorylated Cdc2. Caspase-3 was also activated, leading to the cleavage of Caspase-3 and PARP. We further explored the possible molecular mechanisms upon MCP treatment in UBC cells. Reduction of galectin-3 was observed and followed with the inactivation of Akt signaling pathway. Of note, galectin-3 knockdown by RNA interference recapitulated the MCP-mediated anti-proliferation, cell cycle arrest and apoptosis. Moreover, oral administration of MCP to the T24 xenograft-bearing nude mice inhibited the tumor growth significantly (P<0.05). Quantification analysis of immunohistochemistry staining for Ki67 and cleaved Caspase-3 confirmed the decrease of proliferation index (P<0.05) and the increase of apoptosis index (P<0.01) in 700 mg/kg MCP-fed UBC xenografts. Using the information from TCGA database, we revealed that the overexpression of galectin-3 was associated with high tumor grade with lymph node metastasis, poor overall survival in UBC patients. Considering the remarkable inhibitory effects of MCP on UBC cell proliferation and survival in vitro and in vivo mainly through galectin-3, which is upregulated in UBCs, MCP may become an attractive agent, as a natural dietary fiber, for prevention and therapy of UBCs. | Tian Fang Dan-dan Liu He-ming Ning Dan Liu Jing-ya Sun Xiao-jing Huang Yu Dong Mei-yu Geng Shi-feng Yun Jun Yan Rui-min Huang | 2018 | Acta Pharmacologica Sinica2018，39，12： | 10 |
| 3 | Research progress on the mechanism of improving peanut yield by single-seed precision sowing显示文摘The contradiction between the supply and demand of edible vegetable oil in China is prominent,and the self-sufficiency rate is less than 35%.Peanut has a very outstanding status in ensuring the security of edible oil and food.The emphasis of increasing peanut yield should be the improvement of pod yield per unit area,because the total yield of peanut has not increased as required.This is attributed to mainly two factors-low increase in the crop productivity and the competition for land for grain and cotton crops.For traditional double-seed sowing pattern,it is difficult to further increase the peanut yield due to the serious contradiction between populations and individuals and the declining population quality under high-yield conditions.Single-seed precision sowing was proven to be a new way to increase the economic coefficient(economic yield/biological yield)with the basic stability of the total biomass,which could make plants distribute evenly,reduce the competition among individuals and attain the full production potential of single plant.In order to reveal the mechanism of increasing peanut yield by single-seed precision sowing,the effects on the ontogenetic development(plant character,physiological characteristic and nutrient utilization)and population structure(population uniformity and photosynthesis,source-sink relationship and yield composition)were systematically expounded.This study reports establishment of the high-yield cultivation technology system with the key technology of single-seed precision sowing and the supporting technology of fertilizing and management.We anticipate its wider application for the improvement of peanut yield. | ZHANG Jia-lei GENG Yun GUO Feng LI Xin-guo WAN Shu-bo | 2020 | Journal of Integrative Agriculture2020，19，8： | 9 |
| 4 | Arbuscular mycorrhizal fungi combined with exogenous calcium improves the growth of peanut(Arachis hypogaea L.)seedlings under continuous cropping显示文摘The growth and yield of peanut are negatively affected by continuous cropping.Arbuscular mycorrhizal fungi(AMF)and calcium ions(Ca^(2+))have been used to improve stress resistance in other plants,but little is known about their roles in peanut seedling growth under continuous cropping.This study investigated the possible roles of the AMF Glomus mosseae combined with exogenous Ca^(2+)in improving the physiological responses of peanut seedlings under continuous cropping.G.mosseae combined with exogenous Ca^(2+)can enhance plant biomass,Ca^(2+)level,and total chlorophyll content.Under exogenous Ca^(2+)application,the F_v/F_m in arbuscular mycorrhizal(AM)plant leaves was higher than that in the control plants when they were exposed to high irradiance levels.The peroxidase,superoxide dismutase,and catalase activities in AM plant leaves also reached their maximums,and accordingly,the malondialdehyde content was the lowest compared to other treatments.Additionally,root activity,and content of total phenolics and flavonoids were significantly increased in AM plant roots treated by Ca^(2+)compared to either G.mosseae inoculation or Ca^(2+)treatment alone.Transcription levels of AhCaM,AhCDPK,AhRAM1,and AhRAM2 were significantly improved in AM plant roots under exogenous Ca^(2+)treatment.This implied that exogenous Ca^(2+)might be involved in the regulation of G.mosseae colonization of peanut plants,and in turn,AM symbiosis might activate the Ca^(2+)signal transduction pathway.The combination of AMF and Ca^(2+)benefitted plant growth and development under continuous cropping,suggesting that it is a promising method to cope with the stress caused by continuous cropping. | CUI Li GUO Feng ZHANG Jia-lei YANG Sha MENG Jing-jing GENG Yun WANG Quan LI Xin-guo WAN Shu-bo | 2019 | Journal of Integrative Agriculture2019，18，2： | 9 |
| 5 | Clinical Application of Chromosome Microarray Analysis in Han Chinese Children with Neurodevelopmental Disorders显示文摘Chromosome microarray analysis(CMA) is a cost-effective molecular cytogenetic technique that has been used as a first-line diagnostic test in neurodevelopmental disorders in the USA since 2011. The impact of CMA results on clinical practice in China is not yet well studied, so we aimed to better evaluate this phenomenon.We analyzed the CMA results from 434 patients in our clinic, and characterized their molecular diagnoses, clinical features, and follow-up clinical actions based on these results. The overall diagnostic yield for our patients was 13.6%(59 out of 434). This gave a detection rate of 14.7%for developmental delay/intellectual disability(DD/ID,38/259) and 12% for autism spectrum disorders(ASDs,21/175). Thirty-three recurrent(n≥2) variants were found, distributed at six chromosomal loci involving known chromosome syndromes(such as DiGeorge, Williams Beuren, and Angelman/Prader-Willi syndromes).The spectrum of positive copy number variants in our study was comparable to that reported in Caucasian populations, but with specific characteristics. Parental origin tests indicated an effect involving a significant maternal transmission bias to sons. The majority of patients with positive results(94.9%) had benefits, allowing earlier diagnosis(36/59), prioritized full clinical management(28/59), medication changes(7/59), a changed prognosis(30/59), and prenatal genetic counseling(15/59). Our results provide information on de novo mutations in Chinese children with DD/ID and/or ASDs. Our data showed that microarray testing provides immediate clinical utility for patients. It is expected that the personalized medical care of children with developmental disabilities will lead to improved outcomes in long-term developmental potential.We advocate using the diagnostic yield of clinically actionable results to evaluate CMA as it provides information of both clinical validity and clinical utility. | Mingyu Xu Yiting Ji Ting Zhang Xiaodong Jiang Yun Fan Juan Geng Fei Li | 2018 | Neuroscience Bulletin2018，34，6： | 8 |
| 6 | A Study of the Technique of Western Blot for Diagnosis of Lyme Disease caused by Borrelia afzelii in China显示文摘Objective To study the technique of Western blot for the diagnosis of Lyme disease caused by Borrelia afzelii in China and to establish the standard criteria by operational procedure. Methods FP1, which is the representative strain of B. afzelii in China, was analyzed by SDS‐PAGE, electro transfer and immunoblotting assays. The molecular weights of the protein bands of FP1 were analyzed by Gel‐Pro analysis software. In a study using 451 serum samples (159 patients with Lyme disease and 292 controls), all observed bands were recorded. The accuracy of the WB as a diagnostic test was established by using the ROC curve and Youden index. Results Criteria for a positive diagnosis of Lyme disease were established as at least one band of P83/100, P58, P39, OspB, OspA, P30, P28, OspC, P17, and P14 in the IgG test and at least one band of P83/100, P58, P39, OspA, P30, P28, OspC, P17, and P41 in the IgM test. For IgG criteria, the sensitivity, specificity and Youden index were 69.8%, 98.3%, and 0.681, respectively; for IgM criteria, the sensitivity, specificity and Youden index were 47%, 94.2%, and 0.412, respectively. Conclusion Establishment of WB criteria for B. afzelii is important in validating the diagnostic assays for Lyme disease in China. | LIU Zhi Yun HAO Qin HOU Xue Xia JIANG Yi GENG Zhen WU Yi Mou WAN Kang Lin | 2013 | Biomedical and Environmental Sciences2013，26，3： | 7 |
| 7 | Pancreatogastrostomy vs pancreatojejunostomy after pancreaticoduodenectomy: An updated meta-analysis of RCTs and our experience显示文摘BACKGROUND Pancreatoduodenectomy(PD)is one of the most important operations in hepatobiliary and pancreatic surgery.AIM To evaluate the advantages and disadvantages of pancreaticojejunostomy(PJ)and pancreaticogastrostomy(PG).METHODS This meta-analysis was performed using Review Manager 5.3.All clinical randomized controlled trials,in which patients underwent PD with pancreaticodigestive tract reconstruction via PJ or PG,were included.RESULTS The search of PubMed,Wanfang Data,EMBASE,and the Cochrane Library provided 125 citations.After further analysis,11 trials were included from nine counties.In all,909 patients underwent PG and 856 underwent PJ.Meta-analysis showed that pancreatic fistula(PF)was a significantly lower morbidity in the PG group than in the PJ group(odds ratio[OR]=0.67,95%confidence interval[CI]:0.53-0.86,P=0.002);however,grades B and C PF was not significantly different between the two groups(OR=0.61,95%CI:0.34-1.09,P=0.09).Postoperative hemorrhage showed a significantly lower morbidity in the PJ group than in the PG group(OR=1.47,95%CI:1.05-2.06,P=0.03).Delayed gastric emptying was not significantly different between the two groups(OR=1.09,95%CI:0.83-1.41,P=0.54).CONCLUSION There is no difference in the incidence of grades B and C PF between the two groups.However,postoperative bleeding is significantly higher in PG than in PJ.Binding PJ or binding PG is a safe and secure technique according to our decades of experience. | Yun Jin Yang-Yang Feng Xiao-Gang Qi Geng Hao Yuan-Quan Yu Jiang-Tao Li Shu-You Peng | 2019 | World Journal of Gastrointestinal Surgery2019，11，7： | 7 |
| 8 | Human movement monitoring and behavior recognition for intelligent sports using customizable and flexible triboelectric nanogenerator显示文摘Effective collection,recognition,and analysis of sports information is the key to intelligent sports,which can help athletes to improve their skills and formulate scientific training plans and competition strategies.At present,wearable electronic devices used for movement monitoring still have some limitations,such as high cost and energy consumption,incompatibility of suitable flexibility and personalized spatial structure,dissatisfactory data analysis methods,etc.In this work,a novel three-dimensionalprinted thermoplastic polyurethane is introduced as the elastic shell and friction layer,and it endows the proposed customizable and flexible triboelectric nanogenerator(CF-TENG)with personalized spatial structure and robust correlation to external pressure.In practical application,it exhibits highly sensitive responses to the joint-bending motion of the finger,wrist,or elbow.Furthermore,a pressure-sensing insole and smart ski pole based on CF-TENG are manufactured to build a comprehensive sports monitoring system to transmit the athletes’motion information from feet and hands through the plantar pressure distribution and ski pole action.To recognize the movement status,the self-developed automatic peak recognition algorithm(P-Find)and machine learning algorithm(subspace K-Nearest Neighbors)were introduced to accurately distinguish the four typical motion behaviors and three primary sub-techniques of cross-country skiing,with accuracy rates of 98.2%and 100%.This work provides a novel strategy to promote the personalized applications of TENGs in intelligent sports. | YANG Yun HOU XiaoJuan GENG WenPing MU JiLiang ZHANG Le WANG XiangDong HE Jian XIONG Ji Jun CHOU XiuJian | 2022 | Science China(Technological Sciences)2022，65，4： | 6 |
| 9 | Wheat breeding history reveals synergistic selection of pleiotropic genomic sites for plant architecture and grain yield显示文摘Diversity surveys of crop germplasm are important for gaining insights into the genomic basis for plant architecture and grain yield improvement,which is still poorly understood in wheat.In this study,we exome sequenced 287 wheat accessions that were collected in the past 100 years.Population genetics analysis identified that 6.7%of the wheat genome falls within the selective sweeps between landraces and cultivars,which harbors the genes known for yield improvement.These regions were asymmetrically distributed on the A and B subgenomes with regulatory genes being favorably selected.Genome-wide association study(GWAS)identified genomic loci associated with traits for yield potential,and two underlying genes,TaARF12 encoding an auxin response factor and TaDEP1 encoding the G-proteinγ-subunit,were located and characterized to pleiotropically regulate both plant height and grain weight.Elite single-nucleotide haplotypes with increased allele frequency in cultivars relative to the landraces were identified and found to have accumulated over the course of breeding.Interestingly,we found that TaARF12 and TaDEP1 function in epistasis with the classical plant height Rht-1 locus,leading to propose a“Green Revolution”-based working model for historical wheat breeding.Collectively,our study identifies selection signatures that fine-tune the gibberellin pathway during modern wheat breeding and provides a wealth of genomic diversity resources for the wheat research community. | Aili Li Chenyang Hao Zhenyu Wang Shuaifeng Geng Meiling Jia Fang Wang Xiang Han Xingchen Kong Lingjie Yin Shu Tao Zhongyin Deng Ruyi Liao Guoliang Sun Ke Wang Xingguo Ye Chengzhi Jiao Hongfeng Lu Yun Zhou Dengcai Liu Xiangdong Fu Xueyong Zhang Long Mao | 2022 | Molecular Plant2022，15，3： | 4 |
| 10 | Malignant solitary fibrous tumor of the pancreas with systemic metastasis: A case report and review of the literature显示文摘BACKGROUND Pancreatic solitary fibrous tumor(SFT) is a rare neoplasm of intermediate biological potential. So far, only 22 cases have been reported since 1999. All the cases, except one, exhibited benign features. Here, we report the first case of malignant pancreatic SFT with typical Doege-Potter syndrome, along with the clinical and pathologic evidence of its systemic metastasis.CASE SUMMARY The patient was a 48-year-old man with a 1-year history of pancreatic and liver masses and refractory hypoglycemia. Increased uptake of the tracer fluorodeoxyglucose(FDG) was found in the liver and bones by fluorine-18 FDG positron emission tomography/computed tomography. After multidisciplinary discussion, a distal pancreatectomy procedure was performed, and histological examination showed a lesion composed of abundant heterogeneous spindle cells with localized necrosis. On immunohistochemistry evaluation, STAT6 was found to be diffusely expressed in the tumor. Based on the overall evidence, the patient was diagnosed with malignant pancreatic SFT with liver and bone metastases.CONCLUSION The diagnosis of malignant SFT requires comprehensive evidence including clinical, immunohistochemistry, and histological features. This case may be presented as a reference for diagnoses and management of malignant pancreatic SFTs with systemic metastasis. | Hao Geng Yu Ye Yun Jin Bai-Zhou Li Yuan-Quan Yu Yang-Yang Feng Jiang-Tao Li | 2020 | World Journal of Clinical Cases2020，8，2： | 4 |
| 11 | Influence of annealing temperatures on microstructure evolution and mechanical properties in a low-carbon steel显示文摘Quenching and partitioning (Q&P) heat treatments with different annealing temperatures and fixed initial quenching tem-peratures were applied to cold-rolled low-carbon steel with the initial microstructure of ferrite and pearlite, aiming to gain the same amount of austenite (preset value) before the partitioning stage. The chemical compositions of the material have been specially designed, containing 1.6 wt.% silicon and 0.8 wt.% aluminum to avoid the precipitation of carbides. The micro-structure evolution of the investigated steel was characterized using a dilatometer, an optical microscope, a scanning electron microscope (SEM), an X-ray diffractometer, an electron backscattered diffraction and transmission electron microscope. Consequently, the microstructure of all samples looks quite similar. At the same time, according to SEM micrographs and dilatometer data, there are competitive reactions in Q&P process, such as the precipitation of carbides, the transformation of bainite and the formation of secondary martensite. Thus, the measured austenite is less than the preset values. Mechanical properties of the material were detected by uniaxial tensile tests. The results indicate that the ultimate tensile strength of the four groups of samples is similar, but the total elongation has a significant downward tendency with the increase in annealing temperatures. After annealing at 840 ℃, the steel possesses great ultimate tensile strength of about 1200 MPa and optimum total elongation of about 20.37% with favorable products of strength and elongation of about 24.35 GPa%. | You-hui Jiang Shun Yao Wei Liu Yun Han Su-peng Liu Geng Tian Ai-min Zhao | 2020 | Journal of Iron and Steel Research(International)2020，27，8： | 3 |
| 12 | Broad Cell Tropism of SADS-CoV In Vitro Implies Its Potential Cross-Species Infection Risk显示文摘Dear Editor,Swine acute diarrhoea syndrome coronavirus (SADS-Co V)is a novel swine enteric coronavirus belonged to the Coronaviridae family,Alphacoronavirus genus (Zhou P et al.2018).The clinical signs of SADS include acute diarrhoea and vomiting,which was similar with the infection of porcine epidemic diarrhea virus (PEDV) and porcine deltacoronavirus (PDCo V)(Wang et al.2019). | Yun Luo Ying Chen Rong Geng Bei Li Jing Chen Kai Zhao Xiao-Shuang Zheng Wei Zhang Peng Zhou Xing-Lou Yang Zheng-Li Shi | 2021 | Virologica Sinica2021，36，3： | 2 |
| 13 | Imbalance of interleukin‐17‐producing CD 4 T cells/regulatory T cells axis occurs in remission stage of patients with hepatitis B virus‐related acute‐on‐chronic liver failure显示文摘 | Geng‐Lin Zhang Dong‐Ying Xie Bing‐Liang Lin Chan Xie Yi‐Nong Ye Liang Peng Shao‐Quan Zhang Yu‐Feng Zhang Qing Lai Jian‐Yun Zhu Ying Zhang Yang‐Su Huang Zhao‐Xia Hu Zhi‐Liang Gao | 2013 | J Gastroenterol Hepatol2013，，3： | 2 |
| 14 | Bergenin, a PPARγ agonist, inhibits Th17 differentiation and subsequent neutrophilic asthma by preventing GLS1-dependent glutaminolysis显示文摘Bergenin is a natural PPARγ agonist that can prevent neutrophil aggregation, and often be used in clinics for treating respiratory diseases. Recent data show that Th17 cells are important for neutrophil aggregation and asthma through secreting IL-17A. In this study, we investigated the effects of bergenin on Th17 differentiation in vitro and subsequent neutrophilic asthma in mice. Naïve T cells isolated from mouse mesenteric lymph nodes were treated with IL-23, TGF-β, and IL-6 to induce Th17 differentiation. We showed that in naïve T cells under Th17-polarizing condition, the addition of bergenin (3, 10, 30 μM) concentration-dependently decreased the percentage of CD4+ IL-17A+ T cells and mRNA expression of specific transcription factor RORγt, and function-related factors IL-17A/F, IL-21, and IL-22, but did not affect the cell vitality and apoptosis. Furthermore, bergenin treatment prevented GLS1-dependent glutaminolysis in the progress of Th17 differentiation, slightly affected the levels of SLC1A5, SLC38A1, GLUD1, GOT1, and GPT2. Glutamine deprivation, the addition of glutamate (1 mM), α-ketoglutarate (1 mM), or GLS1 plasmid all significantly attenuated the above-mentioned actions of bergenin. Besides, we demonstrated that bergenin (3, 10, and 30 μM) concentration-dependently activated PPARγ in naïve T cells, whereas PPARγ antagonist GW9662 and siPPARγ abolished bergenin-caused inhibition on glutaminolysis and Th17 differentiation. Furthermore, we revealed that bergenin inhibited glutaminolysis by regulating the level of CDK1, phosphorylation and degradation of Cdh1, and APC/C-Cdh1-mediated ubiquitin-proteasomal degradation of GLS1 after activating PPARγ. We demonstrated a correlation existing among bergenin-affected GLS1-dependent glutaminolysis, PPARγ, “CDK1-APC/C-Cdh1” signaling, and Th17 differentiation. Finally, the therapeutic effect and mechanisms for bergenin-inhibited Th17 responses and neutrophilic asthma were confirmed in a mouse model of neutrophilic asthma by administration of GW9662 or GLS1 overexpression plasmid in vivo. In conclusion, bergenin repressed Th17 differentiation and then alleviated neutrophilic asthma in mice by inhibiting GLS1-dependent glutaminolysis via regulating the “CDK1-APC/C-Cdh1” signaling after activating PPARγ. | Ling Yang Yun Zheng Yu-meng Miao Wen-xin Yan Yan-zhi Geng Yue Dai Zhi-feng Wei | 2022 | Acta Pharmacologica Sinica2022，43，4： | 2 |
| 15 | Histones released by NETosis enhance the infectivity of SARS-CoV-2 by bridging the spike protein subunit 2 and sialic acid on host cells显示文摘Neutrophil extracellular traps(NETs)can capture and kill viruses,such as influenza viruses,human immunodeficiency virus(HIV),and respiratory syncytial virus(RSV),thus contributing to host defense.Contrary to our expectation,we show here that the histones released by NETosis enhance the infectivity of SARS-CoV-2,as found by using live SARS-CoV-2 and two pseudovirus systems as well as a mouse model.The histone H3 or H4 selectively binds to subunit 2 of the spike(S)protein,as shown by a biochemical binding assay,surface plasmon resonance and binding energy calculation as well as the construction of a mutant S protein by replacing four acidic amino acids.Sialic acid on the host cell surface is the key molecule to which histones bridge subunit 2 of the S protein.Moreover,histones enhance cell-cell fusion.Finally,treatment with an inhibitor of NETosis,histone H3 or H4,or sialic acid notably affected the levels of sgRNA copies and the number of apoptotic cells in a mouse model.These findings suggest that SARS-CoV-2 could hijack histones from neutrophil NETosis to promote its host cell attachment and entry process and may be important in exploring pathogenesis and possible strategies to develop new effective therapies for COVID-19. | Weiqi Hong Jingyun Yang Jun Zou Zhenfei Bi Cai He Hong Lei Xuemei He Xue Li Aqu Alu Wenyan Ren Zeng Wang Xiaohua Jiang Kunhong Zhong Guowen Jia Yun Yang Wenhai Yu Qing Huang Mengli Yang Yanan Zhou Yuan Zhao Dexuan Kuang Junbin Wang Haixuan Wang Siyuan Chen Min Luo Ziqi Zhang Tiangi Lu Li Chen Haiying Que Zhiyao He Qiu Sun Wei Wang Guobo Shen Guangwen Lu Zhiwei Zhao Li Yang Jinliang Yang Zhenling Wang Jiong Li Xiangrong Song Lunzhi Dai Chong Chen Jia Geng Maling Gou Lu Chen Haohao Dong Yong Peng Canhua Huang Zhiyong Qian Wei Cheng Changfa Fan Yuquan Wei Zhaoming Su Aiping Tong Shuaiyao Lu Xiaozhong Peng Xiawei Wei | 2022 | Cellular & Molecular Immunology2022，19，5： | 2 |
| 16 | Study of spatial distribution for the active ingredient in ibuprofen tablet based on near-infrared micro-imaging technology显示文摘The NIR micro-images of ibuprofen tablets were collected in this research.Compare correlation imaging and principal component analysis(PC A) with histogram were applied to acquire the spatial distribution of ibuprofen granule.The result indicated that a similar distribution trend can be acquired by both of the two methods mentioned above;the information of PC2 results from ibuprofen mainly since the correlation coefficient between PC2 loading vector and the NIR spectrum of ibuprofen is 0.9930.The result of PCA indicated that the information of PC2 results from ibuprofen mainly for both the low and the high content of ibuprofen in the tablets.The correlation coefficient between the data of the two PC2 loading vectors of the low and the high content of ibuprofen in the tablets is 0.9998,which indicates that the result of PCA is stable and reliable. | Dong Wang Yun Sheng Ding Jin Li Cao Yue He Sheng Feng Ye Shun Geng Min | 2011 | Chinese Chemical Letters2011，22，11： | 2 |
| 17 | IFN-αinhibits HBV transcription and replication by promoting HDAC3-mediated de-2-hydroxyisobutyrylation of histone H4K8 on HBV cccDNA minichromosome in liver显示文摘The epigenetic modification of hepatitis B virus(HBV)covalently closed circular DNA(cccDNA)plays a crucial role in cccDNA transcription and viral persistence.Interferon-α(IFN-α)is a pivotal agent against HBV cccDNA.However,the mechanism by which IFN-αmodulates the epigenetic regulation of cccDNA remains poorly understood.In this study,we report that IFN-α2b enhances the histone deacetylase 3(HDAC3)-mediated de-2-hydroxyisobutyrylation of histone H4 lysine 8(H4K8)on HBV cccDNA minichromosome to restrict the cccDNA transcription in liver.By screening acetyltransferases and deacetylases,we identified that HDAC3 was an effective restrictor of HBV transcription and replication.Moreover,we found that HDAC3 was able to mediate the de-2-hydroxyisobutyrylation of H4K8 in HBV-expressing hepatoma cells.Then,the 2-hydroxyisobutyrylation of histone H4K8(H4K8hib)was identified on the HBV cccDNA minichromosome,promoting the HBV transcription and replication.The H4K8hib was regulated by HDAC3 depending on its deacetylase domain in the system.The low level of HDAC3 and high level of H4K8hib were observed in the liver tissues from HBV-infected human liver-chimeric mice.The levels of H4K8hib on HBV cccDNA minichromosome were significantly elevated in the liver biopsy specimens from clinical hepatitis B patients,which was consistent with the high transcriptional activity of cccDNA.Strikingly,IFN-α2b effectively facilitated the histone H4K8 de-2-hydroxyisobutyrylation mediated by HDAC3 on the HBV cccDNA minichromosome in primary human hepatocytes and hepatoma cells,leading to the inhibition of HBV transcription and replication.Our finding provides new insights into the mechanism by which IFN-αmodulates the epigenetic regulation of HBV cccDNA minichromosome. | Li-na Zhao Hong-feng Yuan Yu-fei Wang Hao-lin Yun Wei Zheng Ying Yuan Yu Geng Man Zhao Lian-yun Feng Xiao-dong Zhang | 2022 | Acta Pharmacologica Sinica2022，43，6： | 2 |
| 18 | Characterization of M-class genome segments of Muscovy duck reovirus S14显示文摘 | ZHANG Yun GUO Dong-chun GENG Hong-wei | 2007 | Virus Res2007，125，1： | 1 |
| 19 | Genotoxicity of Three Avermectins on Polypedates megacephalus Tadpoles Using the Comet Assay显示文摘Avermectins are a new class of macrocyclic lactones derived from mycelia of the soil actinomycete, and are used as effective agricultural pesticides and antiparasitic agents. However, run-off from crops treated with avermectins may contaminate various bodies of water, and accumulated to certain concentrations to impact the development of aquatic animals. Here, we tested the genotoxicity of three avermectins(abamectin, ABM; ivermectin, IVM; and emamectin benzoate, EMB) on Polypedates megacephalus tadpoles by the alkaline single-cell gel electrophoresis assay. Tadpoles were treated for 48 h in the laboratory with different concentrations of these three agents, 0.006, 0.012, 0.018, 0.024, 0.030 mg/L for ABM, 0.003, 0.006, 0.009, 0.012, 0.015 mg/L for IVM and 0.04, 0.06, 0.08, 0.10, 0.12 mg/L for EMB, and then measured their DNA damage by the Comet assay tail factor %. The concentrations of resulted in highly significant increases in DNA damage of the tadpoles were found above the concentration threshold of 0.012 mg/L ABM, 0.003 mg/L IVM and 0.06 mg/L EMB and linear correlations between the intensity of DNA damage and the concentrations of these three avermectins. Our results showed clearly that avermectins caused dose dependent DNA damage on amphibian tadpoles, and there might be a control on the misuse of avermectins. | Baorong GENG Lingling ZHANG Yun JIANG Xiuping HUANG Jinmei DAI | 2016 | Asian Herpetological Research2016，7，4： | 1 |
| 20 | The effect of SARS-CoV-2 infection on human embryo early development:a multicenter prospective cohort study显示文摘Dear Editor,Severe acute respiratory syndrome coronavirus 2(SARSCo V-2)infection has swept the globe for 3 years(Zhou et al.,2020).With the nationwide relaxation of controls on the coronavirus disease 2019(COVID-19)epidemic since December 2022 in China,fertility and in vitro fertilization(IVF)centers are receiving increasing numbers of infected patients. | Xiaolei Chen Huangcong Shi Cheng Li Wanxia Zhong Linlin Cui Wenjun Zhang Ling Geng Kuona Hu Mei Fang Daimin Wei Junhao Yan Yun Sun Keliang Wu Han Zhao Zi-Jiang Chen | 2023 | Science China(Life Sciences)2023，66，7： | 1 |
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