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| 1 | Serum levels of microRNAs can specifically predict liver injury of chronic hepatitis B显示文摘AIM:To investigate whether circulating microRNAs (miRNAs) can serve as molecular markers to predict liver injury resulted from chronic hepatitis B (CHB). METHODS:The profiles of serum miRNA expression were first generated with serum samples collected from 10 patients with CHB and 10 healthy donors (Ctrls) by microarray analysis. The levels of several miRNAs were further quantitated by real-time reverse transcriptionpolymerase chain reaction with serum samples from another 24 CHB patients and 24 Ctrls. Serum samples of 20 patients with nonalcohlic steatohepatitis (NASH) were also included for comparison. The comparison in the levels of miRNAs between groups (CHB, NASH and Ctrl) was analyzed with Mann-Whitney Utest. The correlation between miRNAs and clinical pathoparameters was analyzed using Spearman correlation analysis or canonical correlation analysis. The receiver-operator characteristic (ROC) curves were also generated to determine the specificity and sensitivity of each individual miRNA in distinguishing patients with CHB from Ctrls. RESULTS:miRNA profile analysis showed that 34 miRNAs were differentially expressed between CHB and Ctrl subjects, in which 12 were up-regulated and 22 down-regulated in CHB subject (fold change > 2.0 and P < 0.01). The median levels of miR-122, -572, -575 and -638 were significantly higher (P < 1.00 × 10 -5 ) while miR-744 significantly lower (P < 1.00 × 10 -6 ) in CHB compared with the Ctrl. The levels of miR-122, -572 and -638 were also higher (P < 1.00 × 10 -3 ) while the level of miR-744 lower in CHB (P < 0.05) than in NASH, although the difference between them was not as significant as that between CHB and Ctrl. ROC curve analysis revealed that the levels of miR-122, -572, -575, -638 and -744 in serum were sensitive and specific enough to distinguish CHB, NASH and Ctrl. Multivariate analysis further showed that the levels of these miRNAs were correlated with the liver function parameters. Most significantly, it was the scatter plot of principal component with the levels of these miRNAs, but not the parameters of liver function, which clearly distinguished CHB, NASH and Ctrl subjects. CONCLUSION:Serum levels of miR-122, -572, -575, -638 and -744 are deregulated in patients with CHB or NASH. The levels of these miRNAs may serve as potential biomarkers for liver injury caused by CHB and NASH. | Hui Zhang Qing-Ya Li Zhi-Zhong Guo Yan Guan Jia Du Yi-Yu Lu Yi-Yang Hu Ping Liu Shuang Huang Shi-Bing Su | 2012 | World Journal of Gastroenterology2012，18，37： | 15 |
| 2 | A Chromosome-Level Genome Assembly of Garlic (Allium sativum) Provides Insights into Genome Evolution and Allicin Biosynthesis显示文摘Garlic,an economically important vegetable,spice,and medicinal crop,produces highly enlarged bulbs and unique organosulfur compounds.Here,we report a chromosome-level genome assembly for garlic,with a total size of approximately 16.24 Gb,as well as the annotation of 57561 predicted protein-coding genes,making garlic the first Allium species with a sequenced genome.Analysis of this garlic genome assembly reveals a recent burst of transposable elements,explaining the substantial expansion of the garlic genome.We examined the evolution of certain genes associated with the biosynthesis of allicin and inulin neoseries-type fructans,and provided new insights into the biosynthesis of these two compounds.Furthermore,a large-scale transcriptome was produced to characterize the expression patterns of garlic genes in different tissues and at various growth stages of enlarged bulbs.The reference genome and large-scale transcriptome data generated in this study provide valuable new resources for research on garlic biology and breeding. | Xiudong Sun Siyuan Zhu Ningyang Li Yi Cheng Jing Zhao Xuguang Qiao Li Lu Shiqi Liu Yanzhou Wang Chan Liu Benping Li Wu Guo Shuang Gao Zemao Yang Fu Li Zheng Zeng Qing Tang Yupeng Pan Mengjiao Guan Jian Zhao Xiaomi ng Lu Huanwe n Meng Zhenlin Han Chun she ng Gao Wenkai Jiang Xing Zhao Shilin Tian Jianguang Su Zhihui Cheng Touming Liu | 2020 | Molecular Plant2020，13，9： | 14 |
| 3 | Malus-metasurface-assisted polarization multiplexing显示文摘Polarization optics plays a pivotal role in diffractive,refractive,and emerging flat optics,and has been widely employed in contemporary optical industries and daily life.Advanced polarization manipulation leads to robust control of the polarization direction of light.Nevertheless,polarization control has been studied largely independent of the phase or intensity of light.Here,we propose and experimentally validate a Malus-metasurface-assisted paradigm to enable simultaneous and independent control of the intensity and phase properties of light simply by polarization modulation.The orientation degeneracy of the classical Malus’s law implies a new degree of freedom and enables us to establish a one-to-many mapping strategy for designing anisotropic plasmonic nanostructures to engineer the Pancharatnam–Berry phase profile,while keeping the continuous intensity modulation unchanged.The proposed Malus metadevice can thus generate a near-field greyscale pattern,and project an independent far-field holographic image using an ultrathin and single-sized metasurface.This concept opens up distinct dimensions for conventional polarization optics,which allows one to merge the functionality of phase manipulation into an amplitudemanipulation-assisted optical component to form a multifunctional nano-optical device without increasing the complexity of the nanostructures.It can empower advanced applications in information multiplexing and encryption,anti-counterfeiting,dual-channel display for virtual/augmented reality,and many other related fields. | Liangui Deng Juan Deng Zhiqiang Guan Jin Tao Yang Chen Yan Yang Daxiao Zhang Jibo Tang Zhongyang Li Zile Li Shaohua Yu Guoxing Zheng Hongxing Xu Cheng-Wei Qiu Shuang Zhang | 2020 | Light(Science & Applications)2020，9，1： | 7 |
| 4 | Development and utilization of a new chemically-induced soybean library with a high mutation density显示文摘Mutagenized populations have provided important materials for introducing variation and identifying gene function in plants. In this study, an ethyl methanesulfonate(EMS)-induced soybean(Glycine max) population,consisting of 21,600 independent M_2 lines, was developed.Over 1,000 M_(4(5))families, with diverse abnormal phenotypes for seed composition, seed shape, plant morphology and maturity that are stably expressed across different environments and generations were identified. Phenotypic analysis of the population led to the identification of a yellow pigmentation mutant, gyl, that displayed significantly decreased chlorophyll(Chl) content and abnormal chloroplast development. Sequence analysis showed that gyl is allelic to Minn Gold, where a different single nucleotide polymorphism variation in the Mg-chelatase subunit gene(ChlI1a) results in golden yellow leaves. A cleaved amplified polymorphic sequence marker was developed and may be applied to marker-assisted selection for the golden yellow phenotype in soybean breeding. We show that the newly developed soybean EMS mutant population has potential for functional genomics research and genetic improvement in soybean. | Zhongfeng Li Lingxue Jiang Yansong Ma Zhongyan Wei Huilong Hong Zhangxiong Liu Jinhui Lei Ying Liu Rongxia Guan Yong Guo Longguo Jin Lijuan Zhang Yinghui Li Yulong Ren Wei He Ming Liu Nang Myint Phyu Sin Htwe Lin Liu Bingfu Guo Jian Song Bing Tan Guifeng Liu Maiquan Li Xianli Zhang Bo Liu Xuehui Shi Sining Han Sunan Hua Fulai Zhou Lili Yu Yanfei Li Shuang Wang Jun Wang Ruzhen Chang Lijuan Qiu | 2017 | Journal of Integrative Plant Biology2017，59，1： | 6 |
| 5 | Chloride channels are involved in sperm motility and are downregulated in spermatozoa from patients with asthenozoospermia显示文摘人的精子当通过女繁殖的道时,从 330 ~ 290 mOsm l-1 遇到渗透的减少。我们试图与 asthenozoospermia 从病人在体积规定和精子活动性评估氯化物隧道的角色。精子用 Percoll 密度坡度被净化。精子体积是被测量向前用流动 cytometry 散布信号。精子活动性用电脑辅助的精子分析(房屋) 被分析。当从一个等渗的答案(330 mOsm l-1 ) 转了到一个张力减退的答案(290 mOsm l-1 ) 时,房间体积没从 normozoospermic 人在精子被改变;但是从 asthenozoospermic 样品在那些增加了。氯化物隧道 blockers, 4,4-diisothiocyanatostilbene-2,2- isulfonic 酸(DIDS ) 或到张力减退的溶液的安息香的酸(NPPB ) 引起了正常精子到的 5-nitro-2-(3-phenylpropylamino ) 的增加胀大,但是没从 asthenozoospermic 精液增加那些的体积。DIDS 和 NPPB 减少了在精液的两个集合的精子活动性取样。正常精子活动性上的 NPPB 的禁止的效果是比在从 asthenozoospermic 样品的精子上强壮得多的。两个精子打表示 ClC-3 氯化物隧道,但是表示在 asthenozoospermic 样品铺平低得多,特别在颈和中间片的区域。从有 asthenozoospermia 的人的精子表明了调整能力,活动性,和 ClC-3 表示层次的更低的体积(特别在颈) 比正常精子。因此,氯化物隧道在精子体积和活动性的规定起重要作用并且是在 asthenozoospermia 的情况下的 downregulated。 | Shan-Wen Liu Yuan Li Li-Li Zou Yu-Tao Guan Shuang Peng Li-Xin Zheng Shun-Mei Deng Lin-Yan Zhu Li-Wei Wang Li-Xin Chen | 2017 | Asian Journal of Andrology2017，19，4： | 3 |
| 6 | Assessment of surface drag coefficient parametrizations based on observations and simulations using the Weather Research and Forecasting model显示文摘海气界面动量交换对台风发展起着重要作用,其估算公式依赖于拖曳系数。本文利用观测和台风模拟试验对八种拖曳系数参数化方案进行系统的评估。结果表明,相对于早期的参数化方案,近些年提出的参数化方案对拖曳系数的描述有了显著的改进,主要体现在高风速条件下。不同的拖曳系数参数化方案对超强台风'海燕'的模拟路径几乎没有影响,但是对其强度、尺寸和结构的模拟结果有显著影响。 | LI Fu-Ning SONG Jin-Bao HE Hai-Lun LI Shuang Li Xia GUAN Shou-De | 2016 | Atmospheric and Oceanic Science Letters2016，9，4： | 3 |
| 7 | Hofmeister Effect-Assisted Strong Natural Biopolymer-Based Hydrogels with Multi-Functions显示文摘Natural biopolymer-based hydrogels have been extensively studied in recent years due to their excellent biocompatibility.However,the preparation of multi-functional and tough natural biopolymer-based hydrogels is still a challenging problem.Herein,a natural biopolymer-based hydrogel is prepared using gelatin and carboxymethyl chitosan(CMCS)through a one-step soaking method.The prepared hydrogel without any synthetic polymers and crosslinking agents has a fully physical crosslinking structure.Due to the hydrophobic interaction brought by the Hofmeister effect,the mechanical properties of soaked hydrogels(tensile stress and strain can reach 3.77 MPa and 1082%)are superior to conventional protein hydrogels.In addition,the prepared gelatin/carboxymethyl chitosan(Gel/CMCS)hydrogels exhibit a variety of appealing properties,including good shape memory,fatigue resistance,electrical conductivity,water retention,drug releasing,antibacterial property,and recyclability.This strategy opens up a new horizon to fabricate hydrogels with excellent mechanical properties and multiple functions,which can extend their applications in the biomedicine area and other related fields. | Yaqian Cai Jiachuan Shi Fangzhe Liu Haichao Li Xinya Man Shuang Guan | 2021 | Chinese Journal of Chemistry2021，39，11： | 3 |
| 8 | PVA/PEG Hybrid Hydrogels Prepared by Freeze-thawing and High Energy Electron Beam Irradiation显示文摘 | ZHAO Chunming LU Xueting HU Qianqian LIU Shuai GUAN Shuang | 2017 | Chemical Research in Chinese Universities2017，33，6： | 2 |
| 9 | Association of Catalase Genotype with Oxidative Stress in the Predication of Colorectal Cancer:Modification by Epidemiological Factors显示文摘Objective This paper aims to assess the interaction between common variations in catalase(CAT) polymorphic gene and environmental factors for antioxidant defense enzyme in modulating individual susceptibility to colorectal cancer(CRC).Methods A case-control study with 880 colorectal cancer cases and 848 controls was conducted to investigate whether variations in the catalase(CAT) gene,one of the genes involved in scavenging oxidative stress,influenced susceptibility to CRC.Results The interaction between life style and genotypes as well as with their effects on colorectal cancer was deduced from the present study.Significant difference(P=0.01) was identified in the distribution of CAT genotype between the colorectal cancer cases and the controls.The CRC cases had significantly lower mean activity than the controls(P<0.01).Correlation analyses revealed statistically significant correlations between CAT activity and CAT genotype(P<0.01).Conclusion The risk of CRC was associated with smoking,low vegetable consumption,high pork and poultry consumptions,and low or high BMI.This is the first study reporting an association of polymorphism CAT-21A>T with colorectal cancer.Low CAT activity was associated with an increased risk of CRC;however,no evidence was found to support an association between CAT-21A>T polymorphism and CRC risk. | CHANG Dong HU Zhang Liang ZHANG Lin ZHAO Ya Shuang MENG Qing Hui GUAN Qing Bai ZHOU Jin PAN Hong Zhi | 2012 | Biomedical and Environmental Sciences2012，25，2： | 2 |
| 10 | Mechanical and Adhesive Properties of Ploy(ethylene glycerol) Diacrylate Based Hydrogels Plasticized with PEG and Glycerol显示文摘 | LI Longyun GUAN Shuang YANG Li QIN Xuzhen FENG Wei | 2018 | Chemical Research in Chinese Universities2018，34，2： | 2 |
| 11 | Mitochondrial protein IF1 is a potential regulator of glucagon-like peptide(GLP-1) secretion function of the mouse intestine显示文摘IF1(ATPIF1) is a nuclear DNA-encoded mitochondrial protein whose activity is inhibition of the F1Fo-ATP synthase to control ATP production.IF1 activity remains unknown in the regulation of GLP-1 activity.In this study,IF1 was examined in the diet-induced obese mice using the gene knockout(If1-KO) mice.The mice gained more body weight on a high fat diet without a change in food intake.Insulin tolerance was impaired,but the oral glucose tolerance was improved through an increase in GLP-1 secretion.The KO mice exhibited an improved intestine structure,mitochondrial superstructure,enhanced mitophagy,reduced apoptosis and decreased adenine nucleotide translocase 2(ANT2) protein in the intestinal epithelial cells together with preserved gut microbiota.The data suggest that GLP-1 secretion was enhanced in the obese If1-KO mice to preserve glucose tolerance through a signaling pathway of ANT2/mitochondria/L-cells/GLP-1/insulin.IF1 is a potential mitochondrial target for induction of GLP-1 secretion in L-cells. | Ying Wang Jiaojiao Zhang Xinyu Cao Yaya Guan Shuang Shen Genshen Zhong Xiwen Xiong Yanhong Xu Xiaoying Zhang Hui Wang Jianping Ye | 2021 | Acta Pharmaceutica Sinica B2021，11，6： | 1 |
| 12 | Organic/inorganic superabso'bent hydrogels based on xylan and montmorillonite 显示文摘 | ZHANG Shuang GUAN Ying FU Gen-que | 2014 | Journal of Nanomaterials2014，65，4： | 1 |
| 13 | Rational application of gefitinib in NSCLC patients with sensitive EGFR mutations based on pharmacokinetics and metabolomics显示文摘Gefitinib has been available in the market for 20 years,but its pharmacokinetic mechanism of response is little known.In this study,we examined the pharmacokinetic and metabolomic profiles in non-small cell lung cancer(NSCLC)patients with sensitive EGFR mutations.A total of 216 advanced NSCLC patients were enrolled,and administered gefitinib at the standard dosage of 250 mg/day,which was established in heterogeneous subjects with non-sensitive mutations.We identified and quantified three main metabolites(named as M1,M2 and M3)in the plasma of patients,the correlations between the concentration of gefitinib/metabolites and efficacy were analyzed.In exploratory and validation set,gefitinib concentration was not correlated with clinical effects.Considering the result that the therapeutic effects of 250 mg/2-day was better than that of 250 mg/day in a multiple center clinical trial,the standard dose might be higher than that for maximal efficacy according to the hypothetical dose-response curve.Among the three metabolites,the IC50 of M2 in HCC827 and PC9 cell lines was significantly lower,and Conc.brain/Conc.plasma of M2 in mice was significantly higher than those of gefitinib,suggesting its higher potential to penetrate blood–brain barrier and might be more effective in the treatment of brain metastatic tumor than gefitinib.Consistently and attractively,higher M2 plasma concentration was found to be correlated with better clinical outcome in patients with brain metastases(the median PFS of CM2<12 ng/mL and CM2≥12 ng/mL were 17.0 and 27.1 months,respectively,P=0.038).The plasma concentration of M2≥12 ng/mL was a strong predictor of the PFS of NSCLC patients.In conclusion,for NSCLC patients with EGFR sensitive mutations,the standard dose is suspectable and could be decreased reasonably.M2 plays an important role in efficacy and may be more effective in the treatment of metastatic tumor than gefitinib. | Wei Feng Xi Chen Shao-xing Guan Hong-lian Ruan Yan Huang Hui-zhen Zhang Yun-peng Yang Wen-feng Fang Hong-yun Zhao Wei Zhuang Shuang Xin You-hao Chen Fei Wang Yue Gao Min Huang Xue-ding Wang Li Zhang | 2022 | Acta Pharmacologica Sinica2022，43，7： | 1 |
| 14 | Bentonite Reinforced Tough Composite Hydrogels as Potential Artificial Articular Cartilage显示文摘 | LU Xueting FENG Wei WANG Honglei HU Qianqian GUAN Shuang GUO Peipei | 2018 | Chemical Research in Chinese Universities2018，34，6： | 1 |
| 15 | Salidroside attenuates LPS-induced pro-inflammatory cytokine responses and improves survival in murine endotoxemia 显示文摘 | Guan Shuang Feng Haihua Song Bocui | 2011 | Int Immunopharmacol2011，11，12： | 1 |
| 16 | Production,purification and activity evaluation of three novel antioxidant peptides obtained from grass carp(Ctenopharyngodon idella)scale waste by microbial protease BaApr1 hydrolysis显示文摘In this study,an alkaline protease BaApr1 from the Bacillus altitudinis W3 was chosen to hydrolysis grass carp(Ctenopharyngodon idella)scales.The hydrolysate of alkaline protease BaApr1 exhibited the best antioxidant activity compared to other protease hydrolysates.The optimal hydrolysis conditions for BaApr1 were an enzyme dosage of 1250 U/g,a hydrolysis time of 7 h,a pH of 9.5 and a temperature of 50℃.Three novel peptides were purified using ultrafiltration,anion exchange chromatography,gel filtration chromatography and ultra-performance liquid chromatography,and their sequences were identified as Tyr-Val-Gln-Ala-Gly-Ala-Ala-Gly-Ala-Ala-Ala-His(SHP2),Val-Lys-Leu-Tyr-Val-Leu-Leu-Val-Pro(SHP4),and Val-Gln-Val-Leu-Ala-Gly-Pro-Val-Val-Lys-Leu-Tyr(SHP5)with molecular weights of 1086.53 Da,1043.69 Da and 1285.79 Da,respectively.Among them,SHP2 exhibited the highest scavenging activity on DPPH;(EC_(50)4.08 mg/mL),ABTS+;(EC_(50)0.23 mg/mL)and HO;(EC_(50)2.78 mg/mL),and the strongest reducing power.Additionally,SHP5 can significantly inhibit lipid peroxidation in the linoleic acid system.In conclusion,three peptides isolated from scales of hydrolysate of grass carp showed great antioxidant activity and might be used as potential food ingredients and pharmaceuticals. | Shuang Li Qiaopeng Tian Tiantian Meng Zhengbing Guan Yujie Cai Xiangru Liao | 2022 | Systems Microbiology and Biomanufacturing2022，2，3： | 1 |
| 17 | Adaptive sliding mode control of eletro hydraulic system with nonlinear parameters 显示文摘 | GUAN Cheng PAN Shuang xia | 2008 | Control Engineering Practice2008，16，11： | 1 |
| 18 | Differentially expressed long noncoding RNAs and regulatory mechanism of LINC02407 in human gastric adenocarcinoma显示文摘BACKGROUND Long noncoding RNAs(lncRNAs)have been identified to play important roles in the development and progression of various tumors,including gastric cancer(GC).However,the molecular role of lncRNAs in GC progression remains unclear.AIM To investigate the differential expression of lncRNAs in human GC and elucidate the function and regulatory mechanism of LINC02407.METHODS The Cancer Genome Atlas database was used to investigate the involvement of lncRNAs in GC.Quantitative real-time polymerase chain reaction was used to estimate the relative expression level of LINC02407 in GC tissues and cells.Functional experiments including CCK8 assay,apoptosis assay,wound healing assay,and transwell assay were used to investigate the effect of LINC02407 on GC cells.Some microRNAs were predicted and verified via bioinformatics analysis and the luciferase reporter system.Predictive analysis and Western blot assay were used to analyze the expression of related proteins.RESULTS Many differentially expressed lncRNAs were identified in GC,and some of them including LINC02407 can affect the survival.LINC02407 was upregulated in tumor tissues compared with adjacent tissues.HGC-27 cells showed the highest LINC02407 expression and HaCaT cells exhibited the lowest expression.Different experiment groups were constructed using LINC02407 overexpressing plasmids and related siRNAs.The results of functional experiments showed that LINC02407 can promote the proliferation,migration,and invasion of GC cells but inhibit apoptosis.Luciferase reporter assay showed that hsa-miR-6845-5p and hsa-miR-4455 was downstream regulated by LINC02407.Western blot analysis showed that adhesion G protein-coupled receptor D1(ADGRD1)was regulated by the LINC02407-miR-6845-5p/miR-4455-ADGRD1 pathways.CONCLUSION LINC02407 plays a role in GC through the LINC02407-miR-6845-5p/miR-4455-ADGRD1 pathways,and thus,it may be an important oncogene and has potential value in GC diagnosis and treatment. | Li-Li Zhou Yan Jiao Hong-Mei Chen Li-Hua Kang Qi Yang Jing Li Meng Guan Ge Zhu Fei-Qi Liu Shuang Wang Xue Bai Yan-Qiu Song | 2019 | World Journal of Gastroenterology2019，25，39： | 1 |
| 19 | Protective effect of brain and muscle arnt-like protein-1 against ethanol-induced ferroptosis by activating Nrf2 in mice liver and HepG2 cells显示文摘Alcohol abuse has recently become a serious health concern worldwide,and the incidence of alcoholic liver disease(ALD)is rapidly increasing with high morbidity and mortality.Ferroptosis is a newly recognized form of regulated cell death caused by the iron-dependent accumulation of lipid peroxidation.Here we showed that the circadian clock protein brain and muscle arnt-like protein-1(BMAL1)in hepatocytes is both necessary and sufficient to protect against ALD by mitigating ferroptosis.U pon exposure to alcohol(5%Lieber-DeCarli liquid alcohol diet for 10 days before binged alcohol with 5 g/kg body weight in vivo,300 mmol/L for 12 h in vitro,respectively),the content of iron,reactive oxygen species(ROS)and malondialdehyde(MDA)was boosted signifi cantly while glutathione(GSH)was decreased that mainly based on the downregulated protein expression of ferritin heavy chain(FTH),ferroportin(FPN),heme oxygenase1(HO-1)and anti-cystine/glutamate antiporter(SLC7A11),while these changes could be abolished by ferroptosis inhibitor Ferrostatin-1[Fer-1(5 mg/kg body weight for 10 days in vivo,10μmol/L for 2 h in vitro,respectively)].Further study indicated that the alcohol could activate the protein expression of BMAL1 which exerts a protective effect against ferroptosis through promoting nuclear factor erythroid 2-related factor 2(Nrf2)translocation into nuclear and subsequently stimulating its downstream proteins FTH,FPN,glutathione peroxidase 4 activity(GPX4),HO-1,SLC7A11,while knockdown of BMAL1 and Nrf2 by RNA interference further downregulated the expression of these protein and thus promoting ferroptosis in response to alcohol.Collectively,our results unveiled that the protective action of BMAL1 during alcohol challenge depends on its ability to activate Nrf2-ARE antiferroptosis pathway and targeting hepatic BMAL1 to dampen hepatic ferroptosis signaling may have therapeutic potential for ALD. | Yanan Zhao Ranran Zhang Ziheng Chen Ziyi Wang Shuang Guan Jing Lu | 2023 | Food Science and Human Wellness2023，12，6： | 0 |
| 20 | Development of a New Cold-Tolerant Maize(Zea mays L.)Germplasm Using the ICE1 Gene from Arabidopsis thaliana显示文摘To develop cold-tolerant maize germplasms and identify the activation of INDUCER OF CRT/DRE-BINDING FACTOR EXPRESSION(ICE1)expression in response to cold stress,RT-PCR was used to amplify the complete open reading frame sequence of the ICE1 gene and construct the plant expression vector pCAMBIA3301-ICE1-Bar.Immature maize embryos and calli were transformed with the recombinant vector using Agrobacterium tumefaciens-mediated transformations.From the regenerated plantlets,three T1 lines were screened and identi-fied by PCR.A Southern blot analysis showed that a single copy of the ICE1 gene was integrated into the maize(Zea mays L.)genomes of the three T1 generations.Under low temperature-stress conditions(4°C),the relative conductivity levels decreased by 27.51%–31.44%,the proline concentrations increased by 12.50%–17.50%,the malondialdehyde concentrations decreased by 16.78%–18.37%,and the peroxidase activities increased by 19.60%–22.89%in the T1 lines compared with those of the control.A real-time quantitative PCR analysis showed that the ICE1 gene was ectopically expressed in the roots,stems,and leaves of the T1 lines.ICE1 positively regulates the expression of the CBF genes in response to cold stress.Thus,this study showed the successful transformation of maize with the ICE1 gene,resulting in the generation of a new maize germplasm that had increased tolerance to cold stress. | Jing Qu Shuang Liu Peng Jiao Zhenzhong Jiang Jianbo Fei Shuyan Guan Yiyong Ma | 2022 | Phyton-International Journal of Experimental Botany2022，91，8： | 0 |
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