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| 1 | Complete genome sequence of the rifamycin SV-producing Amycolatopsis mediterranei U32 revealed its genetic characteristics in phylogeny and metabolism显示文摘Amycolatopsis mediterranei 被用于 rifamycin 的工业规模生产,它在 antimycobacterial 治疗起一个重要作用。作为类 Amycolatopsis 的首先定序的染色体, 236 715 底配对的包括 10 的紧张 U32 的染色体,最大的原核生物的染色体之一到目前为止曾经被定序。不同于在 streptomycetes 发现的线性拓扑学,这个染色体是圆形的,特别地类似于 Saccharopolyspora erythraea 和奴卡氏菌属 farcinica,在发展史和分类代表他们的靠近的关系。尽管在 A 预言了 9 228 编码蛋白质的基因。mediterranei 染色体 S 与那些分享了 orthologs 的最大的数字。erythraea,它被 Streptomyces coelicolor 而非 N 出人意料地跟随。farcinica,显示不同新陈代谢的特征经由改编演变到多样的生态的壁龛。除类似于那的一个核心区域以外在 streptomycetes 普通,有典型核心特征的一个新奇“伪核心”在非核心区域以内被定义,在 21 从 26 基因为第二等的代谢物生产聚类的总数被定位的地方。位于核心的 rifamycin 生合成基因簇为 rifamycin SV 的变换编码细胞色素 P450 酶必需品到 B,由比作 rifamycin 生产 B 紧张 S699 的高度相应的簇揭示了并且进一步由基因互补证实了。A 的 genomic 信息。mediterranei 表明在看起来复杂的规章的机制的控制下面不仅为各种各样的碳来源和无机的氮混合物的广泛的利用而且为新陈代谢的中介的有效 funneling 安排进第二等的抗菌素合成进程的一个新陈代谢的网络。 | Wei Zhao Yi Zhong Hua Yuan Jin Wang Huajun Zheng Ying Wang Xufeng Cen Feng Xu Jie Bai Xiaobiao Han Gang Lu Yongqiang Zhu Zhihui Shao Han Yan Chen Li Nanqiu Peng Zilong Zhang Yunyi Zhang Wei Lin Yun Fan Zhongjun Qin Yongfei Hu Baoli Zhu Shengyue Wang Xiaoming Ding Guo-Ping Zbao | 2010 | Cell Research2010，20，10： | 10 |
| 2 | Experimental study on MSW gasification and melting technology显示文摘以便开发市政的稳固的浪费(MSW ) 气化和融化的技术,二预备试验和一个原则综合实验分别地被完成。当时, MSW 的气化特征在 500 750 ° C 被学习等价比率(嗯) 是 0.2 0.5 使用使流体化床的 gasifier。当温度是 550 700 ° C 时并且嗯是 0.2 0.4, syngas 的低热价值(LHV ) 到达 4000 12000 kJ/Nm3。苍蝇灰的融化的特征用一个改正床炉子在 1100 1460 ° C 被调查。超过 99.9% 戴奥辛能被分解,这被证明并且很重金属当温度是 1100 1300 ° C 时,能被团结。原则综合实验在使流体化床的气化和打漩融化系统被执行。MSW 在 550 650 ° C 高效地被气化,打漩融化炉子稳定地并且过去在 1200 1300 ° C 坚持说 95% 苍蝇灰能被打漩融化炉子抓住。结果提供了实际经验和基本数据开发 MSW 气化和融化的技术。 | XIAO Gang JIN Bao-sheng ZHONG Zhao-ping CHI Yong NI Ming-jiang CEN Ke-fa XIAO Rui HUANG Ya-ji HUANG He | 2007 | Journal of Environmental Sciences2007，19，11： | 7 |
| 3 | Preoperative diabetes as a protective factor for pancreatic fistula after pancreaticoduodenectomy: a meta-analysis显示文摘BACKGROUND: The role of diabetes mellitus (DM) in pancreatic fistula (PF) or clinical relevant PF (CR-PF) after pancreaticoduodenectomy (PD) or pylorus-preserving pancreaticoduodenectomy (PPPD) is unclear. We conducted a meta-analysis to investigate the relationship between DM and PF or CR-PF. | Xiang Xia Chen Huang Gang Cen Zheng-Jun Qiu | 2015 | Hepatobiliary & Pancreatic Diseases International2015，14，2： | 7 |
| 4 | Study on pyrolysis and gasification of wood in MSW显示文摘 | NI Ming-jiang XIAO Gang CHI Yong YAN Jian-hua MIAO Qi ZHU Wen-li CEN Ke-fa | 2006 | Journal of Environmental Sciences2006，18，2： | 6 |
| 5 | Expression of a New hemA Gene from Agrobacterium radiobacter in Escherichia coli for 5-Aminolevulinate Production显示文摘A new hemA gene encoding 5-aminolevulinate (ALA) synthase was cloned from Agrobacterium ra- diobacter zju-0121. The ALA synthase catalyzes the pyridoxal phosphate-dependent condensation of succinyl coen- zyme A (succinyl-CoA) and glycine to produce ALA. Four plasmids carrying the A, radiobacter hemA gene were transformed into different E. coli strains. The effects of both genetic and physiological factors on the expression of ALA synthase and ALA production were studied. The results indicated that the final intracellular activity of ALA synthase and the production of ALA in different expression systems varied largely. Among them, the recombinant E. coli BL21 (DE3) harboring the expression plasmid pET28-A. R-hemA was the most suitable one. The effects of isopropyl-β-D-thiogalactopyranoside (IPTG) addition time, IPTG concentration, culture temperature and the initial concentration of precursors and glucose on the ALA production were also evaluated. The expressed ALA synthase accounted for about 23.7% of the intracellular soluble protein. The highest specific activity of ALA syn- thase was 13.8nmol·min-1·mg-1 of intracellular soluble protein. In the batch culture of the recombinant E. coli, the extracellular ALA concentration reached 0.9 g·L-1. | LIU Xiaoxia(刘晓侠) LIN Jianping(林建平) QIN Gang(秦钢) CEN Peilin(岑沛霖) | 2005 | Chinese Journal of Chemical Engineering2005，13，4： | 4 |
| 6 | MiR-301a transcriptionally activated by HIF-2αpromotes hypoxiainduced epithelial-mesenchymal transition by targeting TP63 in pancreatic cancer显示文摘BACKGROUND Pancreatic cancer(PC)is one of the deadliest cancers worldwide.PC metastasis involves a complex set of events,including epithelial-mesenchymal transition(EMT),that increase tumor cell invasiveness.Recent evidence has shown that hypoxia is a major EMT regulator in pancreatic cancer cells and facilitates metastasis;however,the mechanisms remain elusive.AIM To investigate the role of miR-301a in hypoxia-induced EMT in PC cells.METHODS Real-time PCR and Western blot analysis were used to detect the expression of miR-301a and EMT markers in PDAC cells cultured in hypoxic and normoxic conditions.Western blot analysis was used to detect the expression of EMT markers in PDAC cells with miR-301a overexpression.Wound healing assay and Transwell assay were used to detect the migration capabilities of PDAC cells with miR-301a overexpression and knockout.Luciferase assay was used to detect the miR-301a promoter and the 3’untranslated region activity of TP63.Orthotopic PC mouse models were used to study the role of miR-301a in metastasis of PDAC cells in vivo.In situ hybridization assay was used to detect the expression of miR-301a in PDAC patient samples(adjacent paratumor and paired tumor tissues).RESULTS Hypoxic environment could directly promote the EMT of PC cells.The expression level of miR-301a was increased in a HIF2αdependent manner in hypoxia-cultured CFPAC-1 and BxPC-3 cells.Overexpression of miR-301a enhanced the hypoxia-induced EMT of PC cells,while knocking out miR-301a result in the suppression of hypoxia-induced EMT.TP63 was a direct target of miR-301a and involved in the metastatic process of PC cells.Furthermore,miR-301a upregulation facilitated PDAC distant metastasis and lymph node metastasis in vivo.Additionally,miR-301a overexpression was indicative of aggressive clinicopathological behaviors and poor prognosis.CONCLUSION The newly identified HIF-2α-miR301a-TP63 signaling pathway may play a crucial role in hypoxia-induced EMT in PDAC cells. | Kun-Dong Zhang Bin Hu Gang Cen Yu-Han Yang Wei-Wei Chen Zeng-Ya Guo Xiao-Feng Wang Qian Zhao Zheng-Jun Qiu | 2020 | World Journal of Gastroenterology2020，26，19： | 4 |
| 7 | Fluidized-bed pyrolysis of waste bamboo显示文摘Bamboo was a popular material substituting for wood,especially for one-off commodity in China. In order to recover energy and materials from waste bamboo,the basic characteristics of bamboo pyrolysis were studied by a thermogravimetric analyzer. It implied that the reaction began at 190~210 °C,and the percentage of solid product deceased from about 25% to 17% when temperature ranged from 400 °C to 700 °C. A lab-scale fluidized-bed furnace was setup to research the detailed properties of gaseous,liquid and solid products respectively. When temperature increased from 400 °C to 700 °C,the mass percent of solid product decreased from 27% to 17% approximately,while that of syngas rose up from 19% to 35%. When temperature was about 500°C,the percentage of tar reached the top,about 31%. The mass balance of these experiments was about 93%~95%. It indicated that three reactions involved in the process: pyrolysis of exterior bamboo,pyrolysis of interior bamboo and secondary pyrolysis of heavy tar. | XIAO Gang NI Ming-jiang HUANG He CHI Yong XIAO Rui ZHONG Zhao-ping CEN Ke-fa | 2007 | Journal of Zhejiang University-Science A(Applied Physics & Engineering)2007，8，9： | 3 |
| 8 | Baf60b-mediated ATM-p53 activation blocks cell identity conversion by sensing chromatin opening显示文摘由系特定的抄写因素的表示的系变换是改变那的 epigenetic 的进程有低效率。一个房间由抵抗系变换的机制大部分是未知的。使用肝特定的抄写因素 Foxa3, Hnf1 和 Gata4 (3TF ) 在老鼠成纤维细胞导致肝的变换,我们证明 3TF 导致了 ATM-p53 小径的强壮的激活,它导致了增长拘捕和房间死亡,和它进一步阻止了肝的变换。尤其是, ATM 激活,独立于 DNA 损坏,对染色质在肝的变换期间开作出回应。由在肝的变换期间描绘早分子的事件,我们发现了那 Baf60b,改变建筑群的 SWI/SNF 染色质的一个成员,连接染色质洞到由便于 ATM 招募到肝的基因 loci 的一块面板的开的染色质区域的 ATM 激活。这些调查结果由在察觉到揭示 ATM-p53 小径的功能使细胞的回答清楚些到系变换染色质洞。 | Shuyi Ji Linying Zhu Yimeng Gao Xiaoran Zhang Yupeng Yan Jin Cen Rongxia Li Rong Zeng Lujian Liao Chunhui Hou Yawei Gao Shaorong Gao Gang Wei Lijian Hui | 2017 | Cell Research2017，27，5： | 2 |
| 9 | Modeling and Generating Realistic Background Traffic by Hybrid Approach显示文摘One of the key challenges in largescale network simulation is the huge computation demand in fine-grained traffic simulation.Apart from using high-performance computing facilities and parallelism techniques,an alternative is to replace the background traffic by simplified abstract models such as fluid flows.This paper suggests a hybrid modeling approach for background traffic,which combines ON/OFF model with TCP activities.The ON/OFF model is to characterize the application activities,and the ordinary differential equations(ODEs) based on fluid flows is to describe the TCP congestion avoidance functionality.The apparent merits of this approach are(1) to accurately capture the traffic self-similarity at source level,(2) properly reflect the network dynamics,and(3) efficiently decrease the computational complexity.The experimental results show that the approach perfectly makes a proper trade-off between accuracy and complexity in background traffic simulation. | QIAN Yaguan GUAN Xiaohui JIANG Ming CEN Gang | 2015 | China Communications2015，12，10： | 2 |
| 10 | Summary of distance measurement based on vision in localization technology 显示文摘 | Zhang Handong Wang Gang Cen Yuwan | 2006 | Power Electronics and Motion2006，3，： | 1 |
| 11 | Effects of Heat Treatment Process Parameters on Microstructure and Mechanical Properties of DP440Cold Rolled Strip显示文摘In order to optimize the production process,improve the production efficiency and accelerate the development and application of the domestic dual-phase steel,the effects of heat treatment process parameters on microstructure and mechanical properties of DP440cold rolled strip were studied by the CAS-300simulated continuous annealing equipment.When the heating rate increased from 5to 100℃/s,both the tensile strength and yield strength increased because of the decreased grain size.When the intercritical annealing temperature increased from 780to 850℃,the martensite content decreased so that the tensile strength decreased first,then increased,and the yield strength increased.When the rapid cooling rate increased from 25to 100℃/s,because the martensite content increased,the tensile strength increased,while the yield strength decreased.When the overaging temperature increased from 260to400℃,the tensile strength decreased,while the yield strength increased.When the overaging time increased from240to 480s,the tensile strength decreased a little,while the yield strength increased a little. | Gang HUO Yi-ming CEN Zhen-xing LI Guo-dong LI Chang-sheng LI | 2014 | Journal of Iron and Steel Research(International)2014，21，5： | 1 |
| 12 | Modeling NO x emissions from coal-fired utility boilers using support vector regression with ant colony optimization显示文摘 | Hao Zhou Jia Pei Zhao Li Gang Zheng Chun Lin Wang Ke Fa Cen | 2011 | Engineering Applications of Artificial Intelligence2011，，1： | 1 |
| 13 | Warming with an underbody warming system reduces intraoperative hypothermia in patients undergoing laparoscopic gastrointestinal surgery: A randomized controlled study显示文摘 | Ying Pu Gang Cen Jing Sun Jin Gong Ying Zhang Min Zhang Xia Wu Junjie Zhang Zhengjun Qiu Fang Fang | 2013 | International Journal of Nursing Studies2013，，： | 1 |
| 14 | Kinetic of wet air oxidation of wastewater from natural fiber web desizing显示文摘 | LEI Le-cheng LIU Gang CEN Pei-lin | 2000 | Chinese J of Chem Eng2000，8，2： | 1 |
| 15 | Factors influencing excretive production of human epidermal growth factor (hEGF) with recombinant Escherichia coli K12 system显示文摘 | Xu Zhinan Liu Gang Cen Peilin W. K. R. Wong | 2000 | Bioprocess Engineering2000，，6： | 1 |
| 16 | Modeling NO x emissions from coal-fired utility boilers using support vector regression with ant colony optimization显示文摘 | Hao Zhou Jia Pei Zhao Li Gang Zheng Chun Lin Wang Ke Fa Cen | 2011 | Engineering Applications of Artificial Intelligence2011，，1： | 1 |
| 17 | Real-Time Analysis and Prediction System for Rail Transit Passenger Flow Based on Deep Learning显示文摘With the rapid development of urban rail transit,rail transit plays an important role in alleviating city congestion.In recent years,with increasing pas-sengerflow,there has been huge pressure on passengerflow management.To address this problem,we propose a novel system to provide real-time statistics and predictions of passengerflow based on big data technology and deep learning technology.Moreover,the passengerflow is visualized efficiently in this system.It can provide refined passengerflow information so that people can make more rational decisions in terms of operation and planning,deploy contingency plans to avoid emergency situations,and integrate passengerflow analysis with train production,scheduling and operation to achieve cost reduction and efficiency enhancement. | Xujun Che Gang Cen Shuhui Wu Jiaming Gu Keying Zhu | 2023 | 国际计算机前沿大会会议论文集2023，，2： | 0 |
| 18 | Morphine Re-arranges Chromatin Spatial Architecture of Primate Cortical Neurons显示文摘The expression of linear DNA sequence is precisely regulated by the three-dimensional(3D)architecture of chromatin.Morphine-induced aberrant gene networks of neurons have been extensively investigated;however,how morphine impacts the 3D genomic architecture of neurons is still unknown.Here,we applied digestion-ligation-only high-throughput chromosome conformation capture(DLO Hi-C)technology to investigate the effects of morphine on the 3D chromatin architecture of primate cortical neurons.After receiving continuous morphine administration for 90 days on rhesus monkeys,we discovered that morphine re-arranged chromosome territories,with a total of 391 segmented compartments being switched.Morphine altered over half of the detected topologically associated domains(TADs),most of which exhibited a variety of shifts,followed by separating and fusing types.Analysis of the looping events at kilobase-scale resolution revealed that morphine increased not only the number but also the length of differential loops.Moreover,all identified differentially expressed genes from the RNA sequencing data were mapped to the specific TAD boundaries or differential loops,and were further validated for changed expression.Collectively,an altered 3D genomic architecture of cortical neurons may regulate the gene networks associated with morphine effects.Our finding provides critical hubs connecting chromosome spatial organization and gene networks associated with the morphine effects in humans. | Liang Wang Xiaojie Wang Chunqi Liu Wei Xu Weihong Kuang Qian Bu Hongchun Li Ying Zhao Linhong Jiang Yaxing Chen Feng Qin Shu Li Qinfan Wei Xiaocong Liu Bin Liu Yuanyuan Chen Yanping Dai Hongbo Wang Jingwei Tian Gang Cao Yinglan Zhao Xiaobo Cen | 2023 | Genomics, Proteomics & Bioinformatics2023，21，3： | 0 |
| 19 | Research on low emission MSW gasification and melting system显示文摘In order to eliminate secondary pollution caused by municipal solid waste(MSW)incineration,a MSW gasification and melting process is proposed.The process is expected to reduce the emission of pollutants,especially heavy-metals and dioxins.In this paper,the combustible components of MSW and simulated MSW were gasified in a lab-scale fluidized bed at 400°C-700°C when the excess air ratio(ER)was between 0.2 and 0.8.The experimental results indicated that the MSW could be gasified effectively in a fluidized bed at approximately 600°C-700°C when excess air ratio was 0.2-0.4.The melting characteristics of two typical fly ash samples from MSW incinerators were investigated.The results indicated that fly ash of pure MSW incineration could be melted at approximately 1,300°C and that of MSW and coal co-combustion could be melted at approximately 1,400°C.When temperature was over 1,100°C,more than 99.9%of the dioxins could be decomposed and most of the heavy-metals could be solidified in the slag.Based on the above experiments,two feasible MSW gasification and mel-ting processes were proposed for low calorific value MSW:(1)sieved MSW gasification and melting system,which was based on an idea of multi-recycle;(2)gasification and melting scheme of MSW adding coal as assistant fuel. | XIAO Gang NI Mingjiang CHI Yong JIN Yuqi ZHANG Jiaquan MIAO Qi CEN Kefa | 2007 | Frontiers of Environmental Science & Engineering2007，1，4： | 0 |
| 20 | HIV-1 Transmission among Injecting Drug Users is Principally Derived from Local Circulating Strains in Guangxi, China显示文摘Objective The mode of human immunodeficiency virus(HIV) transmission via injection drug use(IDU)still exists, and the recent shift in IDU-related transmission of HIV infection is largely unknown. The purpose of this study was to analyze the spatiotemporal sources and dynamics of HIV-1 transmission through IDU in Guangxi.Methods We performed a molecular epidemiological investigation of infections across Guangxi from2009 to 2019. Phylogenetic and Bayesian time-geographic analyses of HIV-1 sequences were performed to confirm the characteristics of transmission between IDUs in combination with epidemiological data.Results Among the 535 subjects, CRF08_BC(57.4%), CRF01_AE(28.4%), and CRF07_BC(10.7%) were the top 3 HIV strains;72.6% of infections were linked to other provinces in the transmission network;93.6% of sequence-transmitted strains were locally endemic, with the rest coming from other provinces,predominantly Guangdong and Yunnan;92.1% of the HIV transmission among people who inject drugs tended to be transmitted between HIV-positive IDUs.Conclusion HIV recombinants were high diversity, and circulating local strains were the transmission sources among IDUs in Guangxi. However, there were still cases of IDUs linked to other provinces.Coverage of traditional prevention strategies should be expanded, and inter-provincial collaboration between Guangxi, Yunnan, and Guangdong provinces should be strengthened. | CEN Ping LIANG Hua Yue YANG Yuan ZHANG Fei YANG Shi Xiong MO Ju Cong FENG Yi HUANG Jie Gang NING Chuan Yi HUANG Chun Yuan YANG Yao LIANG Na LIANG Bing Yu YE Li LIANG Hao | 2023 | Biomedical and Environmental Sciences2023，36，5： | 0 |
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