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| 1 | Cadmium accumulation and tolerance of two castor cultivars in relation to antioxidant systems显示文摘To investigate the effects of Cd on tolerance and antioxidant activities of castor, two different castor(Ricinus communis L.) cultivars(Zibo No. 5 and Zibo No. 8) were used for a hydroponic experiment(0, 1 and 2 mg/L Cd) and a pot experiment using Cd-contaminated soil(34 mg/kg) with the addition of ethylenedinitrilotetraacetic acid(EDTA). The results indicated that there were significant differences between the two cultivars with respect to Cd uptake in shoots(113–248 mg/kg for Zibo No. 5 and 130–288 mg/kg Zibo No. 8), biomass tolerance indexes(64.9%–74.6% for Zibo No. 5 and 80.1%–90.9% for Zibo No. 8) in the hydroponic experiment and survival rates(0% for Zibo No. 5 and 100% for Zibo No. 8)determined by the addition of EDTA in the pot experiment, suggesting that Zibo No. 8 has higher tolerance than Zibo No. 5. Moreover, the castor cultivars have low bioconcentration factors(4.80% for Zibo No. 5 and 5.43% for Zibo No. 8) and low translocation factors(<1%).Consequently, Zibo No. 8 can participate in Cd phytostabilization in highly Cd-polluted areas. The results indicated that glutathione(GSH) as a non-enzymatic antioxidant, and antioxidant enzymes including superoxide dismutase(SOD), catalase(CAT) and guaiacol peroxidase(GPX), were cultivar- and dose-dependent. The higher tolerance of Zibo No. 8compared with Zibo No. 5 can be attributed to the higher GSH levels in the root and higher GPX activity in the leaf. | Hanzhi Zhang Qingjun Guo Junxing Yang Tongbin Chen Guangxu Zhu Marc Peters Rongfei Wei Liyan Tian Chunyu Wang Deyun Tan Jie Ma Gangming Wang Yingxin Wan | 2014 | Journal of Environmental Sciences2014，26，10： | 15 |
| 2 | WHEAT STRIPE RUST AND INTEGRATION OF SUSTAINABLE CONTROL STRATEGIES IN CHINA显示文摘Stripe (yellow) rust caused by Puccinia striiformis f. sp. tritici occurs in almost allwheat-producing regions of the world. Severe countrywide epidemics in Chinahave caused substantial yield losses. Growing resistant cultivars is the beststrategy to control this disease but the pathogen can overcome resistance inwheat cultivars. The high variation in the virulence of the pathogen combinedwith the large areas of susceptible wheat cultivars enables the pathogenpopulation to increase rapidly and disperse over long distances under favorableenvironmental conditions, resulting in severe pandemics within croppingseasons. Current stripe rust control measures are based on many years ofresearch including the underlying epidemiology regarding year-to-year survivalof the pathogen, pathways of pathogen dispersal within seasons and years, therole of P. striiformis sexual hybridization, the use of resistance sources inbreeding programs, and year-round surveillance of national wheat crops that arepresent in different parts of the country throughout the year. All these strategiesdepend on accurate prediction of epidemics, more precise use of fungicides tomeet national requirements and better deployment of resistance genes. Newideas with potential application in sustainable protection of stripe rust includenegative regulatory gene editing, resistance gene overexpression and biologicalcontrol based on microbiomes. | Qingdong ZENG Jie ZHAO Jianhui WU Gangming ZHAN Dejun HAN Zhensheng KANG | 2022 | Frontiers of Agricultural Science and Engineering2022，9，1： | 5 |
| 3 | Protocol for analyzing protein liquid-liquid phase separation显示文摘Numerous cellular functions occur in spatially and temporally confined regions. Recent studies have shown that membrane-less organelles and compartments in the cell are assembled via liquid-liquid phase separation (LLPS). In vitro LLPS assays using recombinant expressed and purified proteins are necessary for us to further understand how the assembly of phase-separated compartments is regulated in cells. However;uniform standards and protocols are lacking for these in vitro studies. Here, we describe a step-by-step protocol commonly used to investigate in vitro LLPS using purified proteins. This protocol includes expression and purification of the studied proteins, induction of LLPS of the purified proteins, and studies of the biophysical properties of the liquid droplets formed by LLPS. These protocols can be easily followed by researchers to investigate the LLPS behaviors of proteins of interest. | Zheng Wang Gangming Zhang Hong Zhang | 2019 | Biophysics Reports2019，5，1： | 3 |
| 4 | Influence of mild hypothermia on vascular endothelial growth factor and infarct volume in brain tissues after cerebral ischemia in rats显示文摘BACKGROUND: It has been demonstrated that mild hypothermia has obvious protective effect on both whole and local cerebral ischemia. However, the definite mechanism is still unclear for the brain protection of mild hypothermia on cerebral edema, inhibiting inflammatory reaction, stabilizing blood brain barrier, etc. OBJECTIVE: To investigate the effect of mild hypothermia on the expression of vascular endothelial growth factor and the infarct volume after cerebral ischemia in rats, and analyze the brain protective mechanism of mild hypothermia. DESIGN: A randomized grouping and controlled animal trial. SETTING: Department of Neurology, People's Hospital of Yunyang Medical College. MATERIALS: Twenty adult male SD rats of clean degree, weighing (250±30) g, were provided by the animal experimental center, School of Medicine, Wuhan University. The kits for SP immunohistochemistry were purchased from Beijing Zhongshan Golden Bridge Biotechnology Co., Ltd. METHODS: The experiments were carried out in the laboratory of Department of Neurology, Renmen Hospital of Wuhan University from May to July 2005. ① The 20 rats were divided randomly into normal temperature group (n =10) and mild hypothermia group (n =10). Models of permanent middle cerebral artery occlusion were established with modified nylon suture embolization. The rats were assessed with the Longa standards: 0 point for without nerve dysfunction; 1 for mild neurological deficit (fore claws could no extend completely); 2 for moderate neurological deficit (circling towards the affected side); 3 for severe neurological deficit (tilting towards the affected side); 4 for coma and unconscious; 1-3 points represented that models were successfully established. The rats of the normal temperature group were fed at room temperature, and those in the mild hypothermia group were induced by hypothermia from 2 hours postoperatively, and the rectal temperature was kept at 34-35 ℃ for 72 hours. ② Measurement of infarct volume: All the rats were anesthetized by intraperitoneal injection overdose sodium pentobarbital 7 days postoperatively, and then the heads were cut down to harvest brain. The brain tissues were placed into -20 ℃ refrigerator for 20 minutes, coronal sections of 2 mm were prepared. The infarct sites were not stained, whereas normal brain tissues were stained as red. The infarct volumes were calculated by using MPLAS-500 multimedia color pathological image&word analytical system. ③ Counting positive cells of vascular endothelial growth factor protein: The brains were harvested by cutting heads, then coronal sections of 2 mm were prepared. Routine dehydration, hyalinization, wax immersion and embedding were performed, then the detected with SP immunohistochemistry, the kits were purchased from Beijing Zhongshan Golden Bridge Biotechnology Co., Ltd. The cells whose cytoplasm was yellow-brown were positive ones, a single sample as a unit, peri-ischemic site and ischemic core were selected, and the corresponding sites in controlateral hemisphere were taken as controls. Five visual fields were selected from each site to be observed under microscope, the cells were counted, and the average number of positive cells was calculated in each group. The numbers of positive cells were determined with the image analytical apparatus. MAIN OUTCOME MEASURES: Number of the positive cells of vascular endothelial growth factor protein; Infarct volume of rat brain tissue. RESULTS: All the 20 rats were involved in the analysis of results. ① Number of positive cells of vascular endothelial growth factor protein in brain tissue: It was obviously lower in the mild hypothermia group than in the normal temperature group [(24.02±5.05), (36.07±2.69) cells/high power visual field, P < 0.01]. ② Comparison of infarct volume of brain tissue: After MCAO, it was obviously smaller in the mild hypothermia group than in the normal temperature group [(153.25±23.14), (253.45±36.21) mm3, P < 0.01]. CONCLUSION: Mild hypothermia can inhibit the expression of vascular endothelial growth factor and decrease the volume of cerebral infarction. The inhibition of mild hypothermia on the expression of vascular endothelial growth factor may be one of the brain protective mechanisms. | Fei Ye1, Gangming Xi1, Biyong Qin1, Shifeng Wang1, Chengyan Li2 1Department of Neurology, People’s Hospital of Yunyang Medical College, Shiyan 442000, Hubei Province, China 2Department of Neurology, Renmin Hospital of Wuhan University, Wuhan 430060, Hubei Province, China | 2006 | Neural Regeneration Research2006，1，9： | 2 |
| 5 | Synthesis and biological evaluation of ^(18)F-FB-NGA as a hepatic asialoglycoprotein receptor PET imaging agent显示文摘Asialoglycoprotein receptor(ASGP-R)is a hepatic membrane receptor that uniquely exists on the surface of mammalian hepatocytes,and has been used as target of liver functional imaging agents for many years.We labeled the Galactosyl-neoglycoalbumin(NGA)with 18F to get a PET molecular probe 18F-FB-NGA and evaluated its ability as a liver functional PET imaging agent.The 18F-FB-NGA was prepared with NGA by conjugation with Nsuccinimidyl-4-18F-fluorobenzoate(18F-SFB)and purified with PD-10 desalting column.The radiolabeling yield and radiochemical purity of 18F-FB-NGA were determined by radio-HPLC.Starting with 18F-F–,the total time for 18F-FB-NGA was about 120±10 min.The decay-corrected radiochemical yield is about 25–30%.The radiochemical purity of purified 18F-FB-NGA was more than 98%.Labeled with 185–1850 MBq 18F-SFB,the specific activity of 18F-FBNGA was estimated to be 7.83–78.3 TBq/mmol.Biodistribution of 18F-FB-NGA in normal mice was investigated after injection through the tail vein.The results showed that the liver accumulated 39.47±3.42 and 12.12±6.11%ID/g at 10 and 30 min after injection,respectively.Dynamic MicroPET images in mice were acquired with and without block after injection of the radiotracer,respectively.High liver activity accumulation was observed at 5 min after injection in normal group.On the contrary,the liver accumulation was significantly lower after block,indicating the specific binding to ASGP-R.18F-FB-NGA is probably a potential PET liver imaging agent. | GU Xiaobo CAI Gangming JIANG Mengjun ZHOU Yaoyuan ZHANG Rongjun | 2013 | Nuclear Science and Techniques2013，24，6： | 2 |
| 6 | Sec61β facilitates the maintenance of endoplasmic reticulum homeostasis by associating microtubules显示文摘 | Yimeng Zhu Gangming Zhang Shaoyu Lin Juanming Shi Hong Zhang Junjie Hu | 2018 | Protein & Cell2018，9，7： | 2 |
| 7 | The effect of surface roughness on the stress adaptation of trabecular architecture around a cylindrical implant显示文摘 | Gangming Luo Stephen Cowin | 1999 | Journal of Biomechanics1999，32，3： | 1 |
| 8 | Genomic analysis,trajectory tracking,and field surveys reveal sources and long-distance dispersal routes of wheat stripe rust pathogen in China显示文摘Identifying sources of phytopathogen inoculum and determining their contributions to disease outbreaks are essential for predicting disease development and establishing control strategies.Puccinia striiformis f.sp.tritici(Pst),the causal agent of wheat stripe rust,is an airborne fungal pathogen with rapid virulence variation that threatens wheat production through its long-distance migration.Because of wide variation in geographic features,climatic conditions,and wheat production systems,Pst sources and related dispersal routes in China are largely unclear.In the present study,we performed genomic analyses of 154 Pst isolates from all major wheat-growing regions in China to determine Pst population structure and diversity.Through trajectory tracking,historical migration studies,genetic introgression analyses,and field surveys,we investigated Pst sources and their contributions to wheat stripe rust epidemics.We identified Longnan,the Himalayan region,and the Guizhou Plateau,which contain the highest population genetic diversities,as the Pst sources in China.Pst from Longnan disseminates mainly to eastern Liupan Mountain,the Sichuan Basin,and eastern Qinghai;that from the Himalayan region spreads mainly to the Sichuan Basin and eastern Qinghai;and that from the Guizhou Plateau migrates mainly to the Sichuan Basin and the Central Plain.These findings improve our current understanding of wheat stripe rust epidemics in China and emphasize the need for managing stripe rust on a national scale. | Yuxiang Li Jichen Dai Taixue Zhang Baotong Wang Siyue Zhang Conghao Wang Jiguang Zhang Qiang Yao Mingju Li Chengyun Li Yuelin Peng Shiqin Cao Gangming Zhan Fei Tao Haifeng Gao Weili Huang Xiaojun Feng Yingwen Bai Zhuoma Qucuo Hongsheng Shang Chong Huang Wancai Liu Jiasui Zhan Xiangming Xu Xianming Chen Zhensheng Kang Xiaoping Hu | 2023 | Plant Communications2023，4，4： | 1 |
| 9 | Ultrasound simulation in bone 显示文摘 | Kaufman JJ Gangming L Siffert RS | 2008 | IEEE Trans UFFC2008，55，6： | 1 |
| 10 | Measure of the mean specific capacity of solid material from room temperature to 800℃显示文摘 | Zhou Lan Li Chenxing Hu Gangming | 1984 | Aerospace Materials and Technology1984，4，： | 1 |
| 11 | Population genetic diversity of Puccinia striiformis f. sp. tritici on different wheat varieties in Tianshui, Gansu Province显示文摘 | Gangming Zhan Hua Zhuang Fuping Wang Guorong Wei Lili Huang Zhensheng Kang | 2013 | World Journal of Microbiology and Biotechnology2013，，1： | 1 |
| 12 | An Algorithm to Find K Shortest Path显示文摘 | Gangming Sun Pin Wang | 2014 | International English Education Research2014，，10： | 1 |
| 13 | Mineralization of chlorpyrifos by co-culture of Serratia and Trichosporon spp 显示文摘 | Xu Gangming Li Yingying Zheng Wei | 2007 | Biotechnology Letters2007，29，10： | 1 |
| 14 | A novel ambigrammatic mycovirus,PsV5,works hand in glove with wheat stripe rust fungus to facilitate infection显示文摘Here we describe a novel narnavirus,Puccinia striiformis virus 5(PsV5),from the devastating wheat stripe rust fungus P.striiformis f.sp.tritici(Pst).The genome of PsV5 contains two predicted open reading frames(ORFs)that largely overlap on reverse strands:an RNA-dependent RNA polymerase(RdRp)and a reverseframe ORF(rORF)with unknown function.Protein translations of both ORFs were demonstrated by immune technology.Transgenic wheat lines overexpressing PsV5(RdRp-rORF),RdRp ORF,or rORF were more susceptible to Pst infection,whereas PsV5-RNA interference(RNAi)lines were more resistant.Overexpression of PsV5(RdRp-rORF),RdRp ORF,or rORF in Fusarium graminearum also boosted fungal virulence.We thus report a novel ambigrammatic mycovirus that promotes the virulence of its fungal host.The results are a significant addition to our understanding of virosphere diversity and offer insights for sustainable wheat rust disease control. | Yanhui Zhang Xiaofei Liang Mengxin Zhao Tuo Qi Hualong Guo Jing Zhao Jie Zhao Gangming Zhan Zhensheng Kang Li Zheng | 2023 | Plant Communications2023，4，3： | 1 |
| 15 | 显示文摘 | Hai H W GangM Hui Q L | 2009 | Phys Rev Lett2009，103，06： | 1 |
| 16 | Mineralization of chlorpyrifos by co-culture of Serratia and Trichosporon spp.显示文摘 | Gangming Xu Yingying Li Wei Zheng Xiang Peng Wen Li Yanchun Yan | 2007 | Biotechnology Letters2007，，10： | 1 |
| 17 | mTOR通过调节PGL颗粒的相变来控制它被自噬降解的新机制显示文摘文章简介细胞自噬是真核生物中重要的降解途径,自噬选择性降解蛋白质聚集体从而维持细胞稳态平衡。自噬异常与多种人类疾病密切相关。课题组之前在研究线虫胚胎发育过程时. | Gangming Zhang Zheng Wang Zhuo Du 张宏 | 2019 | 科学新闻2019，0，2： | 0 |
| 18 | Improving word vector model with part-of-speech and dependency grammar information显示文摘Part-of-speech(POS)and dependency grammar(DG)are the basic components of natural language processing.However,current word vector models have not made full use of both POS information and DG information,and hence the models’performances are limited to some extent.The authors first put forward the concept of POS vector,and then,based on continuous bag-of-words(CBOW),constructed four models:CBOW+P,CBOW+PW,CBOW+G,and CBOW+G+P to incorporate POS information and DG information into word vectors.The CBOW+P and CBOW+PW models are based on POS tagging,the CBOW+G model is based on DG parsing,and the CBOW+G+P model is based on POS tagging and DG parsing.POS information is integrated into the training process of word vectors through the POS vector to solve the problem of the POS similarity being difficult to measure.The POS vector correlation coefficient and distance weighting function are used to train the POS vector as well as the word vector.DG information is used to correct the information loss caused by fixed context windows.Dependency relations weight is used to measure the difference of dependency relations.Experiments demonstrated the superior performance of their models while the time complexity is still kept the same as the base model of CBOW. | Chunhui Deng Gangming Lai Huifang Deng | 2020 | CAAI Transactions on Intelligence Technology2020，5，4： | 0 |
| 19 | Vici综合征的致病蛋白EPG5介导自噬小体与晚期内体/溶酶体融合过程显示文摘人类自噬基因EPG5突变会导致多重系统紊乱Vici综合扯。这篇论文证明了EPG5作为Rab7的效应蛋白,特异性介导自噬小体与晚期内体/溶酶体之间的融合。 | Zheng Wang Guangyan Miao Xue Xue Xiangyang Guo Chongzhen Yuan Zhaoyu Wang Gangming Zhang Yingyu Chen Du Feng Junjie Hu 张宏 | 2017 | 科学新闻2017，19，4： | 0 |
| 20 | Determination of heterozygosity for avirulence/virulence loci through sexual hybridization of Puccinia striiformis f. sp. tritici显示文摘Wheat stripe rust caused by Puccinia striiformis f. sp. tritici is one of the most devastating diseases of wheat worldwide and resistant cultivars are vital for its management. Therefore, investigating the heterozygosity of the pathogen is important because of rapid virulence changes in isolates heterozygous for avirulence/virulence.An isolate of P. striiformis f. sp. tritici was selfed on Berberis shensiana to determine the heterozygosity for avirulence/virulence loci. One hundred and twenty progeny isolates obtained from this selfing were phenotyped using 25 lines of wheat containing Yrgenes and genotyped with 96 simple sequencing repeat markers, with51 pathotypes and 55 multi-locus genotypes being identified. All of these were avirulent on lines with Yr5,Yr10, Yr15, Yr24 and Yr26 and virulent on lines with Yr17,Yr25 and YrA, indicating that the parental isolate was homozygously avirulent or homozygously virulent for these loci. Segregation was found for wheat lines with Yr1,Yr2, Yr4, Yr6, Yr7, Yr8, Yr9, Yr27, Yr28, Yr32, Yr43, Yr44,YrExp2, YrSp, YrTr1, YrTye and YrV23. The 17 cultivars to which the Pst was identified as heterozygous with respect to virulence/avirulence should not be given priority in breeding programs to obtain new resistant cultivars. | Yuan TIAN Gangming ZHAN Xia LU Jie ZHAO Lili HUANG Zhensheng KANG | 2017 | Frontiers of Agricultural Science and Engineering2017，4，1： | 0 |
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