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| 1 | Birth of fertile bimaternal offspring following intracytoplasmic injection of parthenogenetic haploid embryonic stem cells显示文摘 | Zhikun Li Haifeng Wan Guihai Feng Leyun Wang Zhengquan He YukaiWang Xiu-Jie Wang Wei Li Qi Zhou Baoyang Hu | 2016 | Cell Research2016，26，1： | 15 |
| 2 | Immunity-and-matrix-regulatory cells derived from human embryonic stem cells safely and effectively treat mouse lung injury and fibrosis显示文摘Lung injury and fibrosis represent the most significant outcomes of severe and acute lung disorders,including COVID-19.However,there are still no effective drugs to treat lung injury and fibrosis.In this study,we report the generation of clinical-grade human embryonic stem cells(hESCs)-derived immunity-and matrix-regulatory cells(IMRCs)produced under good manufacturing practice requirements,that can treat lung injury and fibrosis in vivo.We generate IMRCs by sequentially differentiating hESCs with serum-free reagents.IMRCs possess a unique gene expression profile distinct from that of umbilical cord mesenchymal stem cells(UCMSCs),such as higher expression levels of proliferative,immunomodulatory and anti-fibrotic genes.Moreover,intravenous delivery of IMRCs inhibits both pulmonary inflammation and fibrosis in mouse models of lung injury,and significantly improves the survival rate of the recipient mice in a dose-dependent manner,likely through paracrine regulatory mechanisms.IMRCs are superior to both primary UCMSCs and the FDA-approved drug pirfenidone,with an excellent efficacy and safety profile in mice and monkeys.In light of public health crises involving pneumonia,acute lung injury and acute respiratory distress syndrome,our findings suggest that IMRCs are ready for clinical trials on lung disorders. | Jun Wu Dingyun Song Zhongwen Li Baojie Guo Yani Xiao Wenjing Liu Lingmin Liang Chunjing Feng Tingting Gao Yanxia Chen Ying Li Zai Wang Jianyan Wen Shengnan Yang Peipei Liu Lei Wang Yukai Wang Liang Peng Glyn Nigel Stacey Zheng Hu Guihai Feng Wei Li Yan Huo Ronghua Jin Ng Shyh-Chang Qi Zhou Liu Wang Baoyang Hu Huaping Dai Jie Hao | 2020 | Cell Research2020，30，9： | 7 |
| 3 | A survey on barrier coverage with sensors显示文摘 | Fan WU Yang GUI Zhibo WANG Xiaofeng GAO Guihai CHEN | 2016 | Frontiers of Computer Science2016，10，6： | 6 |
| 4 | SMART: A Strengthened Model of Access Router Tree for Multihoming in Nested Mobile Network显示文摘 | HUANG Songhua WU Xiaobing LU Yin HUANG Hao CHEN Guihai | 2009 | Chinese Journal of Electronics2009，18，1： | 4 |
| 5 | Domesticated cynomolgus monkey embryonic stem cells allow the generation of neonatal interspecies chimeric pigs显示文摘Blastocyst complementation by pluripotent stem cell(PSC)injection is believed to be the most promising method to generate xenogeneic organs.However,ethical issues prevent the study of human chimeras in the late embryonic stage of development.Primate embryonic stem cells(ESCs),which have similar pluripotency to human ESCs,are a good model for studying interspecies chimerism and organ generation.However,whether primate ESCs can be used in xenogenous grafts remains unclear.In this study,we evaluated the chimeric ability of cynomolgus monkey(Macaca fascicularis)ESCs(cmESCs)in pigs,which are excellent hosts because of their many similarities to humans.We report an optimized culture medium that enhanced the anti-apoptotic ability of cmESCs and improved the development of chimeric embryos,in which domesticated cmESCs(D-ESCs)injected into pig blastocysts differentiated into cells of all three germ layers.In addition,we obtained two neonatal interspecies chimeras,in which we observed tissue-specific D-ESC differentiation.Taken together,the results demonstrate the capability of D-ESCs to integrate and differentiate into functional cells in a porcine model,with a chimeric ratio of 0.001-0.0001 in different neonate tissues.We believe this work will facilitate future developments in xenogeneic organogenesis,bringing us one step closer to producing tissue-specific functional cells and organs in a large animal model through interspecies blastocyst complementation. | Rui Fu Dawei Yu Jilong Ren Chongyang Li Jing Wang Guihai Feng Xuepeng Wang Haifeng Wan Tianda Li Libin Wang Ying Zhang Tang Hai Wei Li Qi Zhou | 2020 | Protein & Cell2020，11，2： | 4 |
| 6 | Can outbred mice be used as a mouse model of mild cognitive impairment?显示文摘Deficits in spatial learning and memory are some of the earliest symptoms in mild cognitive im-pairment (MCI). However, there are few valid MCI animal models available to evaluate putative therapeutic strategies. The aim of this study was to obtain a natural animal model of MCI. Outbred Kunming (aged 5 and 12.5 months) and ICR (7 and 12 months) mice were utilized in the present study. Morris water maze and radial six-arm water maze (RAWM) were simultaneously used to evaluate impaired spatial learning and memory in middle-aged mice (approximately 12 months of age). Compared with younger mice in the respective groups, the middle-aged mice suffered visible impairment of spatial memory in the Morris water maze and RAWM, and mild spatial learning deficiency occurred in the RAWM study alone. Thus outbred Kunming and ICR mice could be utilized as a natural animal model for MCI, in particular for memory impairment studies. | Fang Wang Wenhua Xu Chao Wang Dewu Huang Guihai Chen | 2010 | Neural Regeneration Research2010，5，21： | 4 |
| 7 | Human embryonic stem cells contribute to embryonic and extraembryonic lineages in mouse embryos upon inhibition of apoptosis显示文摘 | Wei Li Baoyang Hu XuepengWang Tianda Li Tongtong Cui Dawei Yu Chao Liu Liyuan Jiang Guihai Feng Lei wang Rui Fu Xinxin Zhang Jie Hao Yukai Wang Liu Wang Qi Zhou | 2018 | Cell Research2018，28，1： | 2 |
| 8 | An Energy-Aware Routing Protocol in Wireless Sensor Networks 显示文摘 | Liu Ming Cao Jiannong Chen Guihai | 2009 | Sensors2009，9，： | 1 |
| 9 | Shuffle-ring:a new constantdegree networks显示文摘 | Francis C M Lau | 1993 | International Journal of Foundations of Computer Science1993，9，1： | 1 |
| 10 | Avoiding energy holes in wireless sensor networks with nonuniform node distribution显示文摘 | Xiaobing W Guihai C Das S K | 2008 | IEEE Transactions on Parallel and Distributed Systems2008，19，： | 1 |
| 11 | Avoiding en-ergy holes in wireless sensor networks with nonuni-form node distribution显示文摘 | Xiaobing Wu Guihai Chen Sajal K Das | 2008 | IEEE Transactions on Par-allel and Distributed System2008，19，5： | 1 |
| 12 | Geochemical characteristic of hydrocarbon compounds in the Hangzhou Bay显示文摘The source and distribution of the organic pollutants such as the oil , n-alkanes籥romatic,and the pesticide in the water column, suspended particulates and surface sediments in the Hangzhou Bay (HB) during winter 1987 and summer 1988 are studied with analytical method of fluorometry, GC, LC, GC-MS, etc.The influence of the tide and salinity on the distribution of dissolved and paniculate organic matter is investigated in this paper. The data show that the highest content of suspending silt, organic matter > and chemical oxygen demand (COD) occurs at salinity 15. This results from the fact that the water front limits the transfer and dispersion of the matter in different water mass, and enriches the suspending silt and organic matter in water column.The running direction of the oil-content equivalue lines is similar to that of the water front, and interrelated closely with the terrestrial materials from the Changjiang River. | Lu Bin,Zhu Guihai,Zheng Shilong,Xiong Jian and Shi Jianzhong Second Institute of Oceanography, State Oceanic Administration, Hangzhou 310012, China | 1993 | Acta Oceanologica Sinica1993，12，1： | 1 |
| 13 | The Harary index of trees显示文摘 | Ilic A Yu Guihai Feng Lihun | 2012 | 21 : 87-1012012，21，： | 1 |
| 14 | On the maximal Harary index of a class of bicyclic graphs显示文摘 | Yu Guihai Feng Lihun | 2010 | (82) :285-2922010，，82： | 1 |
| 15 | An unequal cluster - based routing pro- tocol in wireless sensor networks 显示文摘 | Guihai C Chengfa L | 2009 | Wireless Netw2009，15，： | 1 |
| 16 | Effects of magnetic field on the sol gel transition of methylcellulose in water显示文摘 | Wang Qiqiang Li Lin Chen Guihai | 2007 | Carbohydrate Polymers2007，70，3： | 1 |
| 17 | s-vertex pancyclic index 显示文摘 | Zhang Lili Shao Yehong Chen Guihai | 2012 | Graphs and Combinatorics2012，28，3： | 1 |
| 18 | Research status on underwater wireless sensor networks显示文摘 | Chen Jinming Chen Guihai Yan Yunpei | | 0，，09： | 1 |
| 19 | An unequal cluster-based routing protocol in wireless sensor networks显示文摘 | Guihai Chen Chengfa Li Mao Ye | 2009 | Wireless Net- works2009，15，2： | 1 |
| 20 | SVFD: a ver- satile online fault detection scheme via checking of stability violation 显示文摘 | YAN Guihai HAN Yinhe Li Xiaowei | 2011 | IEEE Transactions on VLSI System2011，19，9： | 1 |
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