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| 1 | Preparation of colloidal gold immunochromatography strip for detection of methamidophos residue显示文摘Methamidophos (Met) is a broad spectrum organophosphorus insecticide and acaricide.Even a trace of its residue is harmful to humans and many animals.In this study,the synthesis and identification of colloidal gold particles and antibody-colloidal gold conjugates were performed,and the preparation of colloidal gold immunochromatography strip was conducted for detection of Met residue.The size of colloidal gold particles was checked using a transmission electron microscope (TEM).The formation of antibody-colloidal gold conjugates was monitored by UV/Vis spectroscopy.The preparation of colloidal gold immunochromatography strips,analysis of Met standard solutions,and other four pesticides and vegetable samples were operated with common methods and principals.The TEM images showed the average diameter of colloidal gold particles was almost the same size: approximately 40.0 nm in diameter.For the conjugation of colloidal gold and monoclonal antibody (MAb),0.03 mg/ml of MAb was confirmed to be the minimum amount for stabilization of colloidal gold.With the prepared colloidal gold immunochromatography test strip to determine the standard Met solution,the results demonstrated a detection limit of approximately 1.0 μg/ml.Cross-reaction indicated that the strip had a high specificity to Met.The results of 10 green vegetable sample tests confirmed that one sample was positive by HPLC analysis.There was evidence to suggest that colloidal gold particles and antibody-colloidal gold conjugates were synthesized successfully.The prepared colloidal gold immunochromatography strip was applicable for preliminary screening of Met residue. | Shi Chenggang Zhao Suqing Zhang Kun Hong Guobao Zhu Zhenyu | 2008 | Journal of Environmental Sciences2008，20，11： | 15 |
| 2 | Comprehensive analysis of the impact of climatic changes on Chinese terrestrial net primary productivity显示文摘Recent climatic changes have affected terrestrial net primary productivity (NPP). This paper presents an investigation of the impact of climatic changes on Chinese terrestrial vegetation NPP by analyzing 18 years’ (1982 to 1999) climatic data and satellite observations of vegetation activity. Results indicate that climatic changes in China have eased some critical climatic constraint on plant growth. (1) From 1982 to 1999, modeled NPP increased by 1.42%·a-1 in water-limited regions of Northwest China, 1.46%·a-1 in temperature-limited regions of Northeast China and Tibet Plateau, and 0.99%·a-1 in radia- tion-limited regions of South China and East China. (2) NPP increased by 24.2%, i.e. 0.76 petagram of carbon (Pg C) over 18 years in China. Changes in climate (with constant vegetation) directly contrib- uted nearly 11.5% (0.36 Pg C). Changes in vegetation (with constant climate) contributed 12.4% (0.40 Pg C), possibly as a result of climate-vegetation feedbacks, changes in land use, and growth stimula- tion from other mechanisms. (3) Globally, NPP declined during all three major El Ni-o events (1982 to 1983, 1987 to 1988, and 1997 to 1998) between 1982 and 2000, but Chinese vegetation productivity re- sponded differently to them because of the monsoon dynamics. In the first three events (1982 to 1983, 1987 to 1988, and 1992), Chinese vegetation NPP declined, while in the later two events (1993, 1997 to 1998) increasing obviously. | ZHU WenQuan PAN YaoZhong YANG XiaoQiong SONG GuoBao | 2007 | Chinese Science Bulletin2007，52，23： | 14 |
| 3 | Relative humidity reconstruction for northwestern China's Altay Mountains using tree-ring δ^(18)O显示文摘Relative humidity is an important factor in water and water vapor feedback cycles.In this study,we established a 222-year annual tree-ring δ^(18)O chronology for Siberian larch(Larix sibirica Ldb.)from the Altay Mountains in northwestern China.Climate response analyses revealed that the relative humidity was the primary factor limiting tree-ring δ^(18)O fractionation.Based on our analysis,tree-ring δ^(18)O can be used to reconstruct the July–August relative humidity based on both a reasonable mechanism of tree-ring δ^(18)O fractionation and a statistically significant regression model.We used this model to reconstruct variations in the July–August relative humidity,and the model explained 47.4% of the total variation in the measured relative humidity data from 1961 to 2011.The relative humidity in the study area increased from 1900 to the 1990s and decreased thereafter.Two regime-shift dry periods were detected during the study period(one from 1817 to 1830 and the other from 2004 to 2011). | Guobao Xu Xiaohong Liu Dahe Qin Tuo Chen Wenzhi Wang Guoju Wu Weizhen Sun Wenling An Xiaomin Zeng | 2014 | Chinese Science Bulletin2014，59，2： | 14 |
| 4 | Moisture variations over the past millennium characterized by Qaidam Basin tree-ring δ^(18)O显示文摘High-resolution tree-ring δ18O chronologies covering the last millennium,although scarce,are essential in understanding patterns of climatic changes in the northeastern region of the Qinghai-Tibetan Plateau.For this study,a tree-ring δ18O chronology with a temporal resolution of 3-years was developed from the long-lived Qilian juniper(Sabina przewalskii Kom.),extending back in time to AD 991.This long δ18O chronology was significantly correlated with the yearly δ18O in tree rings during the common period from 1800 to 2006,and was an effective proxy for relative humidity during the growing season.A low-frequency moisture pattern signified the occurrence of a slight drought during the Medieval Climate Anomaly,a marked occurrence of a wet period during the Little Ice Age,and a trend in increasing moisture levels,although lower than average,alongside the Twentieth Century warming trend.Comparisons to other hydroclimatic reconstructions indicate that this tree-ring18O chronology serves as a reliable paleo-humidity proxy for the Qaidam Basin as well as documenting details of past humidity levels in the region. | WANG WenZhi LIU XiaoHong XU GuoBao SHAO XueMei QIN DaHe SUN WeiZhen AN WenLing ZENG XiaoMin | 2013 | Chinese Science Bulletin2013，58，32： | 14 |
| 5 | Study on the spatial differences and its time lag effect on climatic factors of the vegetation in the Longitudinal Range-Gorge Region显示文摘这篇文章包括温度和降水分析 NDVI 和二个气象学的数据的关联,并且探查回答植被的敏感到温度和降水变化的空间模式。由构造 NDVIr 索引,在在纵的范围峡区域(LRGR ) 的植被盖子和陆地使用之间的关系被揭示。结论如下:(1 ) 时间落后那植被回答到温度和降水变化的效果被证明。不同区域有相应于最大的关联系数的不同延期时间。根据降水和温度联合的反应时间,也就是,三种温度降水形式被定义在一样的时间的降水和温度,在温度以前的降水和在降水以前的温度。(2 ) 敏感的空间分发,哪个对温度的植被回答,被几个高、低的散布关联中心描绘。与温度,二个感觉迟钝的区域和二条敏感的带不同被形成介绍植被的反应给降水。(3 ) NDVIr 被构造,它能完美地在 LRGR 反映在气象学的因素和陆地使用状况之间的相应关系。 | BAO YaJing SONG GuoBao LI ZhengHai GAO JiXi LU HaiYan WANG HaiMei CHENG Yan XU Tian | 2007 | Chinese Science Bulletin2007，52，A02： | 12 |
| 6 | Draft genome sequence of cauliflower(Brassica oleracea L.var.botrytis)provides new insights into the C genome in Brassica species显示文摘Cauliflower is an important variety of Brassica oleracea and is planted worldwide.Here,the high-quality genome sequence of cauliflower was reported.The assembled cauliflower genome was 584.60 Mb in size,with a contig N50 of 2.11 Mb,and contained 47,772 genes;56.65%of the genome was composed of repetitive sequences.Among these sequences,long terminal repeats(LTRs)were the most abundant(32.71%of the genome),followed by transposable elements(TEs)(12.62%).Comparative genomic analysis confirmed that after an ancient paleohexaploidy(γ)event,cauliflower underwent two whole-genome duplication(WGD)events shared with Arabidopsis and an additional whole-genome triplication(WGT)event shared with other Brassica species.The present cultivated cauliflower diverged from the ancestral B.oleracea species~3.0 million years ago(Mya).The speciation of cauliflower(~2.0 Mya)was later than that of B.oleracea L.var.capitata(approximately 2.6 Mya)and other Brassica species(over 2.0 Mya).Chromosome no.03 of cauliflower shared the most syntenic blocks with the A,B,and C genomes of Brassica species and its eight other chromosomes,implying that chromosome no.03 might be the most ancient one in the cauliflower genome,which was consistent with the chromosome being inherited from the common ancestor of Brassica species.In addition,2,718 specific genes,228 expanded genes,2 contracted genes,and 1,065 positively selected genes in cauliflower were identified and functionally annotated.These findings provide new insights into the genomic diversity of Brassica species and serve as a valuable reference for molecular breeding of cauliflower. | Deling Sun Chunguo Wang Xiaoli Zhang Wenlin Zhang Hanmin Jiang Xingwei Yao Lili Liu Zhenghua Wen Guobao Niu Xiaozheng Shan | 2019 | Horticulture Research2019，6，1： | 6 |
| 7 | Chemiluminescence and electrochemiluminescence applications of metal nanoclusters显示文摘Due to strong photoluminescence,extraordinary photostability,excellent biocompatibility,and good water-solubility,metal nanoclusters have attracted enormous attention since discovered.They are found to be novel fluorescence labels for biological applications and environmental monitoring.Recently the chemiluminescence(CL) or electrochemiluminescence(ECL) of metal nanoclusters has received increasing attention.This review covers recent vibrant developments in this field of the past 5 years,and highlights different functions of metal nanoclusters in various CL and ECL systems,such as luminophores,catalysts,and quenchers.Latest synthetic methods of metal nanoclusters used in CL or ECL are also summarized.Furthermore,we discuss some perspectives and critical challenges of this field in the near future. | Shuang Han Zhichao Zhang Suping Li Liming Qi Guobao Xu | 2016 | Science China Chemistry2016，59，7： | 5 |
| 8 | Spatial distribution regularity and influence factors of population density in the LRGR显示文摘联合 GIS 措施和统计方法,这份报纸选择地文学的因素和气候的因素在 LRGR 在人口密度上学习影响力量和它所有因素的功能的形式。另外,这份报纸与影响人口密度分发的因素在 LRGR 模仿人口密度分发的地位。调查结果显示下列情形:(1 ) 所有自然因素在 LRGR,而是影响程度和形式在人口密度的空间模式上有影响是相当不同的。地文学的因素和交通网络密度在人口密度上有大影响,当象温度和降水的如此的因素几乎没在人口分发上有小影响时。在人口密度和交通网络密度之间的关系假定否定、积极的线性关联趋势,当水系统密度和斜坡的影响看起来独立是指数的函数和对数函数时。(2 ) 人口在整个 LRGR 被散布并且当因为自然条件的影响的不可分的散布和本地集中,呈现如此的特征。因此,一条高人口密度带在 LRGR 的中间被形成了,并且一个高人口密度补丁在 LRGR 的南方形成。(3 ) 在 LRGR 在人口密度和人的活动索引(HAI ) 之间有一种好线性回归关系,它反映在场的那个人口密度罐头在 LRGR 的自然生态系统上的人工的骚乱。(4 ) 人口密度的增加导致 NDVI 索引的减少。 | SONG GuoBao LI ZhengHai BAO YaJing LU HaiYan GAO JiXi WANG HaiMei XU Tian CHENG Yan | 2007 | Chinese Science Bulletin2007，52，A02： | 4 |
| 9 | Increased mercury pollution revealed by tree rings from the China's Tianshan Mountains显示文摘Mercury (Hg)can be transported globally by the atmospheric circulation and it accumulates and biomagnifies along food chains [1,2].Due to its threats to human health and wildlife when converted to the neurotoxin methyl mercury,variations in Hg concentration in various ecosystems were paid more attention by scientists recently.Natural processes such as geothermal activities, volcanic activities,and weathering of Hg-containing rocks,can release a mass of Hg to the environment.Anthropogenic emissions had become the main atmospheric pollution source since the Industrial Revolution.A recent study indicated that half of the Hg presented in atmosphere was sourced from human activities and about 73%total mercury was released after 1850[3].Aiming to reduce the impact of Hg to environmental and human health,UNEP (United Nations Environment Programme)passed the Minamata Convention in 2013to limit Hg emission through contro|ling technologies on air pollution. | Huhu Kang Xiaohong Liu Junming Guo Guobao Xu Guoju Wu Xiaomin Zeng Bo Wang Shichang Kang | 2018 | Science Bulletin2018，63，20： | 4 |
| 10 | Induction of Wnt signaling antagonists and p21-activated kinase enhances cardiomyocyte proliferation during zebrafish heart regeneration显示文摘Heart regeneration occurs by dedifferentiation and proliferation of pre-existing cardiomyocytes(CMs).However,the signaling mechanisms by which injury induces CM renewal remain incompletely understood.Here,we find that cardiac injury in zebrafish induces expression of the secreted Wnt inhibitors,including Dickkopf 1(Dkkl),Dkk3,secreted Frizzled-related protein 1(sFrpl),and sFrp2,in cardiac tissue adjacent to injury sites.Experimental blocking of Wnt activity via Dkkl overexpression enhances CM proliferation and heart regeneration,whereas ectopic activation of Wnt8 signaling blunts injury-induced CM dedifferentiation and proliferation.Although Wnt signaling is dampened upon injury,the cytoplasmic β-catenin is unexpectedly increased at disarrayed CM sarcomeres in myocardial wound edges.Our analyses indicated that p21-activated kinase 2(Pak2)is induced at regenerating CMs,where it phosphorylates cytoplasmic β-catenin at Ser 675 and increases its stability at disassembled sarcomeres.Myocardial-specific induction of the phospho-mimeticβ-catenin(S675E)enhances CM dedifferentiation and sarcomere disassembly in response to injury.Conversely,inactivation of Pak2 kinase activity reduces the Ser 675-phosphorylatedβ-catenin(pS675-β-catenin)and attenuates CM sarcomere disorganization and dedifferentiation・Taken together,these findings demonstrate that coordination of Wnt signaling inhibition and Pak2/pS675-βYatenin signaling enhances zebrafish heart regeneration by supporting CM dedifferentiation and proliferation. | Xiangwen Peng Kaa Seng Lai Peilu She Junsu Kang Tingting Wang Guobao Li Yating Zhou jianjian Sun Daqing Jin Xiaolei Xu Lujian Liao Jiandong Liu Ethan Lee Kenneth D.Poss Tao P.Zhong | 2021 | Journal of Molecular Cell Biology2021，13，1： | 3 |
| 11 | Method of Electric Powertrain Matching for Battery-powered Electric Cars显示文摘The current match method of electric powertrain still makes use of longitudinal dynamics, which can't realize maximum capacity for on-board energy storage unit and can't reach lowest equivalent fuel consumption as well. Another match method focuses on improving available space considering reasonable layout of vehicle to enlarge rated energy capacity for on-board energy storage unit, which can keep the longitudinal dynamics performance almost unchanged but can't reach lowest fuel consumption. Considering the characteristics of driving motor, method of electric powertrain matching utilizing conventional longitudinal dynamics for driving system and cut-and-try method for energy storage system is proposed for passenger cars converted from traditional ones. Through combining the utilization of vehicle space which contributes to the on-board energy amount, vehicle longitudinal performance requirements, vehicle equivalent fuel consumption level, passive safety requirements and maximum driving range requirement together, a comprehensive optimal match method of electric powertrain for battery-powered electric vehicle is raised. In simulation, the vehicle model and match method is built in Matlab/simulink, and the Environmental Protection Agency (EPA) Urban Dynamometer Driving Schedule (UDDS) is chosen as a test condition. The simulation results show that 2.62% of regenerative energy and 2% of energy storage efficiency are increased relative to the traditional method. The research conclusions provide theoretical and practical solutions for electric powertrain matching for modern battery-powered electric vehicles especially for those converted from traditional ones, and further enhance dynamics of electric vehicles. | NING Guobao XIONG Lu ZHANG Lijun YU Zhuoping | 2013 | Chinese Journal of Mechanical Engineering2013，26，3： | 3 |
| 12 | Blind Equalization Algorithm Based on MCMA and DSE-CMA显示文摘A new blind equalization algorithm based on the modi- fied constant modulus algorithm (MCMA) and dithered signed- error constant modulus algorithm (DSE-CMA) is proposed. This dithered signed-error MCMA (DSE-MCMA) can not only reduce the computational complexity, but also recover the phase rotation in the complex channel. Simulation results have verified the analysis and indicated the good property of DSE-MCMA. | GENG Nian XUE Ni RU Guobao | 2008 | Wuhan University Journal of Natural Sciences2008，13，1： | 2 |
| 13 | Analysis of land use change and its driving force in the Longitudinal Range-Gorge Region显示文摘关于陆地使用度,陆地使用变化率以及各种各样的陆地使用的地区性的差别的研究录入西南纵的范围峡区域(LRGR ) 被执行了在上地区性并且县规模。从社会经济的看法,我们也在研究区域分析了陆地使用变化的开车机制和各种各样的驱动力的相应空间范围。研究结果显示在地区性的规模上,农田,森林和草地在 LRGR,和草地严厉地衰退的陆地使用表演的结构的变化填写陆地使用的主要身体,农田增加和森林保持稳定。在县规模上,陆地使用被那陆地使用水平描绘是相对低的,并且陆地使用度,全面变化率和相对变化在不同陆地使用类型之中评价仍然存在。由分析,在 LRGR 的陆地使用变化被投资主要驾驶人力和物理资源,社会经济产量水平,自然灾难骚乱的紧张和工人的教育水平的紧张。然而, regionalism 在不同驱动力类型的影响范围存在。 | LI ZhengHai SONG GuoBao LU HaiYan BAO YaJing GAO JiXi WANG HaiMei XU Tian CHENG Yan | 2007 | Chinese Science Bulletin2007，52，A02： | 2 |
| 14 | Electrochemiluminescence Imaging Techniques for Analysis and Visualizing显示文摘Electrochemiluminescence(ECL)-based imaging analysis is a dominant method to inspect the electrode composition,to study electrochemical reaction kinetics at a microscopic level,and also a rapid emerging technology in bioanalysis with high spatiotemporal resolutions,high-throughput and visualization characteristics.In comparison with other imaging microscopes,an optical excitation is not involved in imaging,thus the approach is free from background noise resulting in a low detec-tion limit.In this review work,the principle of ECL,its unique natures compared to other luminescence techniques were briefed at first.Then after,the progress and basic principles of ECL imaging were summarized.Furthermore,recent and representative advances of ECL imaging for visualizing and sensing applications were reviewed.Finally,the perspectives in ECL imaging for further perspective were discussed. | Tadesse Haile Fereja Fangxin Du Chao Wang Dmytro Snizhko Yiran Guan Guobao Xu | 2020 | Journal of Analysis and Testing2020，4，2： | 2 |
| 15 | Video Polyp Segmentation: A Deep Learning Perspective显示文摘We present the first comprehensive video polyp segmentation(VPS)study in the deep learning era.Over the years,developments in VPS are not moving forward with ease due to the lack of a large-scale dataset with fine-grained segmentation annotations.To address this issue,we first introduce a high-quality frame-by-frame annotated VPS dataset,named SUN-SEG,which contains 158690colonoscopy video frames from the well-known SUN-database.We provide additional annotation covering diverse types,i.e.,attribute,object mask,boundary,scribble,and polygon.Second,we design a simple but efficient baseline,named PNS+,which consists of a global encoder,a local encoder,and normalized self-attention(NS)blocks.The global and local encoders receive an anchor frame and multiple successive frames to extract long-term and short-term spatial-temporal representations,which are then progressively refined by two NS blocks.Extensive experiments show that PNS+achieves the best performance and real-time inference speed(170 fps),making it a promising solution for the VPS task.Third,we extensively evaluate 13 representative polyp/object segmentation models on our SUN-SEG dataset and provide attribute-based comparisons.Finally,we discuss several open issues and suggest possible research directions for the VPS community.Our project and dataset are publicly available at https://github.com/GewelsJI/VPS. | Ge-Peng Ji Guobao Xiao Yu-Cheng Chou Deng-Ping Fan Kai Zhao Geng Chen Luc Van Gool | 2022 | Machine Intelligence Research2022，19，6： | 2 |
| 16 | Seed-mediated growth method for high-quality noble metal nanocrystals显示文摘This review highlights work from the authors' laboratory on the recent development of seed-mediated growth method for noble metal nanocrystals. The seed-mediated growth method has become one of the most efficient and versatile methods for synthe-sizing high-quality noble metal nanocrystals. The seed-mediated growth method can separate the nucleation and growth stages of metal nanocrystals, and thus provide better control over the size, size distribution, and crystallographic evolution of metal nanocrystals. Because of its high controllability, the seed-mediated growth method is especially promising in providing mechanistic insights into the growth mechanisms of noble metal nanocrystals. In this review, the thermodynamic and kinetic parameters for the nucleation and growth of noble metal nanocrystals are systematically summarized. Mechanistic understanding of these parameters is provided. These studies provide useful guidelines for the rational design and synthesis of novel noble metal nanocrystals with high quality. | NIU WenXin ZHANG Ling XU GuoBao | 2012 | Science China Chemistry2012，55，11： | 2 |
| 17 | Preliminary Evaluation on Resources Enhancement of Artificial Reef in the East Corner of Zhelang Shanwei显示文摘In order to analyze the improvement of fish resource,the trawl net and gill net were used in the background survey and investigation in the artificial reef area and control area of the east of corner was discussed in Zhelang Shanwei in January 2003 and April 2010 respectively.Margalef species richness index,Shannon-Winener diversity index and Pileou uniformity index were chosen to analyze the multiformity of biological population and community structure.Resource density index of trawl net investigation and catch rate of gill net were chosen to estimate the variance of biomass.The results of the trawl net showed that the resources density of the artificial area increased by 3.078 times,and numbers of species increased by 1.875 times,squilla being the dominant group instead of the fish,and the first dominant species being squilla oratoria(Oratosquilla oratoria),which increased by 4.372 times before the artificial reef dropped.The results of the gill net showed that the catch rate of the artificial area increased by 12.043 times,numbers of species increased by 1.333 times,the dominant group was fish,it increased greatly,the first dominant species was golden small fish(Sardinella aurita),which increased by 13.035 times before the artificial reef dropped.Both the results of trawl net and gill net indicated that the effect of fish assemblage were evident,and the community structure had improved greatly.It showed a wonderful effect on conservation and assemblage of fishes resource.The artificial reefs developed a new resource enhancement system in the east corner of Zhelang Shanwei accomplished basically. | Lichen FANG Pimao CHEN Guobao CHEN Yong TANG Huarong YUAN Xue FENG | 2013 | Asian Agricultural Research2013，5，9： | 2 |
| 18 | Structure and properties of nano- hydroxyapatite/polymer composite scaffolds for bone tissue engineering 显示文摘 | Wei Guobao Ma P X | 2004 | Biomaterials2004，25，20： | 1 |
| 19 | Journal of dalian university Molecular Mechanics Study on Stereo-Chemistry of β-Elemene Chinese journal of chroma to graphy显示文摘 | Cheng Guobao Hu Jiehan Gong Guoliang Dai Jianying | 2001 | Dalian Of Daily University2001，19，2： | 1 |
| 20 | Novel phosphors of Eu^3+,Tb^3+ or Bi3^+ activated Gd2GeO5: Eu^3+显示文摘 | Guo Peimin Zhao Fei Li Guobao | 2003 | Joumal of Luminescence2003，105，1： | 1 |
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