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| 1 | Osthole inhibits proliferation of human breast cancer cells by inducing cell cycle arrest and apoptosis显示文摘Recent studies have revealed that osthole,an active constituent isolated from the fruit of Cnidium monnieri(L.) Cusson,a traditional Chinese medicine,possesses anticancer activity.However,its effect on breast cancer cells so far has not been elucidated clearly.In the present study,we evaluated the effects of osthole on the proliferation,cell cycle and apoptosis of human breast cancer cells MDA-MB 435.We demonstrated that osthole is effective in inhibiting the proliferation of MDA-MB 435 cells,The mitochondrion-mediated apoptotic pathway was involved in apoptosis induced by osthole,as indicated by activation of caspase-9 and caspase-3 followed by PARP degradation.The mechanism underlying its effect on the induction of G1 phase arrest was due to the up-regulation of p53 and p21 and down-regulation of Cdk2 and cyclin D1 expression.Were observed taken together,these findings suggest that the anticancer efficacy of osthole is mediated via induction of cell cycle arrest and apoptosis in human breast cancer cells and osthole may be a potential chemotherapeutic agent against human breast cancer. | Lintao Wang Yanyan Peng Kaikai Shi Haixiao Wang Jianlei Lu Yanli Li Changyan Ma | 2015 | The Journal of Biomedical Research2015，29，2： | 12 |
| 2 | Recent Study of Drag Embedment Plate Anchors in China显示文摘试验性、理论的研究拖最近在天津执行的埋入板锚大学在这篇研究论文被总结,它包含与学习相关的一系列重要话题拖锚。为测量轨道和运动方向的技术在土壤拖锚,为测量动人的埋入的技术削尖并且逆行链状的形状嵌入拖线,穿入机制和运动学的行为拖锚,最终的埋入深度拖锚,有任意的吸虫节的锚的运动方向,反向的链状的性质嵌入拖线, interactional 性质在之间拖锚和安装现在的工作显著地在设计和分析减少无常拖埋入板抛锚,并且对改进这种新类型的申请有益在近海的设计拖锚。 | Haixiao Liu | 2012 | Journal of Marine Science and Application2012，11，4： | 7 |
| 3 | Antagonism of Protease-Activated Receptor 4 Protects Against Traumatic Brain Injury by Suppressing Neuroinflammation via Inhibition of Tab2/NF-κB Signaling显示文摘Traumatic brain injury(TBI)triggers the activation of the endogenous coagulation mechanism,and a large amount of thrombin is released to curb uncontrollable bleeding through thrombin receptors,also known as protease-activated receptors(PARs).However,thrombin is one of the most critical factors in secondary brain injury.Thus,the PARs may be effective targets against hemorrhagic brain injury.Since the PAR1 antagonist has an increased bleeding risk in clinical practice,PAR4 blockade has been suggested as a more promising treatment.Here,we explored the expression pattern of PAR4 in the brain of mice after TBI,and explored the effect and possible mechanism of BMS-986120(BMS),a novel selective and reversible PAR4 antagonist on secondary brain injury.Treatment with BMS protected against TBI in mice.mRNA-seq analysis,Western blot,and qRT-PCR verification in vitro showed that BMS significantly inhibited thrombin-induced inflammation in astrocytes,and suggested that the Tab2/ERK/NF-κB signaling pathway plays a key role in this process.Our findings provide reliable evidence that blocking PAR4 is a safe and effective intervention for TBI,and suggest that BMS has a potential clinical application in the management of TBI. | Jianing Luo Xun Wu Haixiao Liu Wenxing Cui Wei Guo Kang Guo Hao Guo Kai Tao Fei Li Yingwu Shi Dayun Feng Hao Yan Guodong Gao Yan Qu | 2021 | Neuroscience Bulletin2021，37，2： | 5 |
| 4 | Features and futures of X-ray free-electron lasers显示文摘Linear accelerator-based free-electron lasers(FELs)are the leading source of fully coherent X-rays with ultra-high peak powers and ultrashort pulse lengths.Current X-ray FEL facilities have proved their worth as useful tools for diverse scientific applications.In this paper,we present an overview of the features and future prospects of X-ray FELs,including the working principles and properties of X-ray FELs,the operational status of different FEL facilities worldwide,the applications supported by such facilities,and the current developments and outlook for X-ray FEL-based research. | Nanshun Huang Haixiao Deng Bo Liu Dong Wang Zhentang Zhao | 2021 | The Innovation2021，2，2： | 4 |
| 5 | Acrolein Induces Systemic Coagulopathy via Autophagy-dependent Secretion of von Willebrand Factor in Mice after Traumatic Brain Injury显示文摘Traumatic brain injury(TBI)-induced coagulopathy has increasingly been recognized as a significant risk factor for poor outcomes,but the pathogenesis remains poorly understood.In this study,we aimed to investigate the causal role of acrolein,a typical lipid peroxidation product,in TBI-induced coagulopathy,and further explore the underlying molecular mechanisms.We found that the level of plasma acrolein in TBI patients suffering from coagulopathy was higher than that in those without coagulopathy.Using a controlled cortical impact mouse model,we demonstrated that the acrolein scavenger phenelzine prevented TBI-induced coagulopathy and recombinant ADAMTS-13 prevented acrolein-induced coagulopathy by cleaving von Willebrand factor(VWF).Our results showed that acrolein may contribute to an early hypercoagulable state after TBI by regulating VWF secretion.mRNA sequencing(mRNA-seq)and transcriptome analysis indicated that acrolein over-activated autophagy,and subsequent experiments revealed that acrolein activated autophagy partly by regulating the Akt/mTOR pathway.In addition,we demonstrated that acrolein was produced in the perilesional cortex,affected endothelial cell integrity,and disrupted the blood-brain barrier.In conclusion,in this study we uncovered a novel pro-coagulant effect of acrolein that may contribute to TBI-induced coagulopathy and vascular leakage,providing an alternative therapeutic target. | Wenxing Cui Xun Wu Dayun Feng Jianing Luo Yingwu Shi Wei Guo Haixiao Liu Qiang Wang Liang Wang Shunnan Ge Yan Qu | 2021 | Neuroscience Bulletin2021，37，8： | 3 |
| 6 | Acrolein Aggravates Secondary Brain Injury After Intracerebral Hemorrhage Through Drp1-Mediated Mitochondrial Oxidative Damage in Mice显示文摘Clinical advances in the treatment of intracranial hemorrhage(ICH)are restricted by the incomplete understanding of the molecular mechanisms contributing to secondary brain injury.Acrolein is a highly active unsaturated aldehyde which has been implicated in many nervous system diseases.Our results indicated a significant increase in the level of acrolein after ICH in mouse brain.In primary neurons,acrolein induced an increase in mitochondrial fragmentation,loss of mitochondrial membrane potential,generation of reactive oxidative species,and release of mitochondrial cytochrome c.Mechanistically,acrolein facilitated the translocation of dynaminrelated protein 1(Drpl)from the cytoplasm onto the mitochondrial membrane and led to excessive mitochondrial fission.Further studies found that treatment with hydralazine(an acrolein scavenger)significantly reversed Drpl translocation and the morphological damage of mitochondria after ICH.In parallel,the neural apoptosis,brain edema,and neurological functional deficits induced by ICH were also remarkably alleviated.In conclusion,our results identify acrolein as an important contributor to the secondary brain injury following ICH.Meanwhile,we uncovered a novel mechanism by which Drpl-mediated mitochondrial oxidative damage is involved in acroleininduced brain injury. | Xun Wu Wenxing Cui Wei Guo Haixiao Liu Jianing Luo Lei Zhao Hao Guo Longlong Zheng Hao Bai Dayun Feng Yan Qu | 2020 | Neuroscience Bulletin2020，36，10： | 3 |
| 7 | Development of a rapid GC-FID method to simultaneously determine triethylamine, diisopropylamine, and 1,1,3,3-tetramethylguanidine residues in an active pharmaceutical ingredient显示文摘A rapid GC-FID method was developed to simultaneously determine residual levels of triethylamine(TEA), 1,1,3,3-tetramethylguanidine(TMG), and diisopropylamine(DIPA) in the synthetic route of an active pharmaceutical ingredient(API). Due to the severe absorption of amines on GC stationary phases,GC columns with various stationary phases were evaluated for optimal peak shape and reproducibility.The final conditions used the Agilent CP-Volamine column to resolve the three amines in 12 min. Various inlet liners were also screened to further improve the sensitivity of the analysis. The Restek Siltek~? liner was selected to achieve the desired detectability for the method. The quantitation limits were 4, 3, and 4 mg/mL for TEA, DIPA, and TMG in the presence of API, respectively. All three amines showed good linearity(r > 0.999) and recoveries(> 90%) over the concentration range of 3 to 16 mg/mL. The testing of residual amines was initially performed at the penultimate stage of the synthesis. However, this work demonstrates that TMG can act as a proton sponge to react with salicylic acid, the counter ion of the penultimate, to form a volatile component that elutes at a different retention time. Consequently, in the final method, these three amines were monitored in the final API to circumvent the matrix interference.Key parameters of the method were qualified per method validation requirements in ICH guidelines. The method was successfully applied for batch testing during development and implemented as an inprocess control procedure at manufacturing sites. | Minshan Shou Haixiao Qiu | 2021 | Journal of Pharmaceutical Analysis2021，11，2： | 2 |
| 8 | Design study for the cascaded HGHG experiment based on the SDUV-FEL显示文摘Cascading stages of high-gain harmonic generation(HGHG) free electron laser(FEL) is a promising way to produce fully coherent X-ray radiation.As a test facility for modern FEL R&D,the Shanghai deep ultraviolet FEL(SDUV-FEL) is now under upgrading for the cascading two stages of HGHG experiment.Since the energy of the electron beam is as low as about 185 MeV after upgrade,the total harmonic number of this two stages HGHG is only 2×2,and the wavelength of the final radiation is 196.5 nm which is the 4th harmonic of the 786 nm seed laser.With help of three-dimensional simulation codes,design studies on the FEL physics for the cascaded HGHG experiment are present based on the parameters of the upgraded SDUV-FEL facility.It is found from the simulation results that the part of the electron beam which has been used in the first stage can still generate powerful radiation in the radiator of the second stage,and this radiation will be difficult to be separated from the radiation generated by the fresh part of the electron beam.To overcome this problem,a novel method based on the energy spectrum of the electron beam is proposed in this paper to demonstrate the 'fresh bunch' technique. | FENG Chao ZHANG Meng LIN GuoQiang GU Qiang DENG HaiXiao CHEN JianHui WANG Dong ZHAO ZhenTang | 2012 | Chinese Science Bulletin2012，57，26： | 2 |
| 9 | Risk factors associated with hemorrhagic cystitis after allogeneic hematopoietic stem cell transplantation显示文摘Hemorrhagic cystitis(HC)is a common complication of allogeneic hematopoietic stem cell transplantation(HSCT).The incidence is about 7%to 68%,and some patients have to suffer a long period of frequent,urgent,and painful urination,which brings great pain.This study aimed to analyze risk factors of HC and its effect on patient survival.We collected the medical records of 859 patients who underwent HSCT at our hospital between August 2016 and August 2020.Patients with and without HC were matched using propensity score matching at a 1:1 ratio based on sex,age,and diagnosis,and logistic regression analyses were used to identify factors associated with HC.We used Kaplan–Meier curves to analyze the survival rates of patients in the HC and non-HC groups.We also analyzed the relationship between BK viral load and the occurrence of HC using receiver operating characteristic curve(ROC)analysis.After propensity score matching,there were 131 patients each in the HC and non-HC groups.In the HC group,89 patients(67.9%)had mild HC(stage II°)and 43(32.1%)had severe HC(stage III–IV).The median interval between stem cell transplantation and HC development was 31(3–244)days.Univariate analysis indicated that donor age,hematopoietic stem cell source,HLA,acute graft-versus-host disease,busulfan,anti-thymocyte globulin(ATG),total body irradiation,cytomegalovirus(CMV)(urine),and BK polyomavirus(BKV)(urine)were significantly associated with HC.ATG,CMV(urine),and BKV(urine)were independent risk factors for HC based on the multivariate analysis.The Kaplan–Meier survival analysis showed no significant difference between the HC and non-HC groups(P=0.14).The 1-and 2-year survival rates in the HC group were 78.4%and 69.6%,respectively,and the corresponding rates in the non-HC group were 84.4%and 80.7%,respectively.ROC analysis indicated that a urine BKV load of 1×10^(7) copies/mL was able to stratify the risk of HC.In conclusion,when the BKV load is>1×10^(7),we needtobe aware of the potential for the development of HC. | Biao Shen Yueshen Ma Haixiao Zhang Mingyang Wang Jia Liu Jiaxin Cao Wenwen Guo Dan Feng Donglin Yang Rongli Zhang Xin Chen Qiaoling Ma Weihua Zhai Sizhou Feng Mingzhe Han Aiming Pang Erlie Jiang 无 | 2022 | Blood Science2022，4，2： | 2 |
| 10 | Control Mechanism of Surface Subsidence and Overburden Movement in Backfilling Mining based on Laminated Plate Theory显示文摘The backfilling mining technology is a type of high-efficiency coal mining technology that is used to address the environmental issues caused by the caving mining technology.In this paper,the mechanical model of symmetrical laminated plate representing the overburden movement caused by the backfilling mining technology is established,and the governing differential equation of the motion of the overburden is derived.The boundary conditions of the mechanical model are put forward,and the analytical solution of the overburden movement and surface subsidence is obtained.The numerical model of the overburden movement and surface subsidence,under mining with backfilling,is established by means of the FLAC3D numerical software,which aims to systematically study the influence of backfilling compactness,mining thickness,and mining depth on the overburden movement and surface subsidence in backfilling mining.When the compactnessηis less than 70%,the overburden movement and surface subsidence is greater,while whenηis greater than 70%,the overburden movement and surface subsidence is reduced significantly.On this basis,the control mechanism of surface subsidence and overburden movement in backfilling mining is obtained.The suitable backfilling compactness is the key to controlling surface subsidence and overburden movement in backfilling mining. | Zhengzheng Cao Feng Du Ping Xu Haixiao Lin Yi Xue Yuejin Zhou | 2017 | Computers, Materials & Continua2017，，3： | 2 |
| 11 | Highly dispersed silica-supported copper nanoparticles prepared by precipitationgel method:a simple but efficient and stable catalyst for glycerol hydrogenolysis 显示文摘 | Huang Zhiwei Cui Fang Kang Haixiao | 2008 | Chem Mater2008，20，15： | 1 |
| 12 | A Globally Convergent BFGS Method with Nonmonotone Line Search for NoncConvex Minimization显示文摘 | Yun Haixiao Hui Juansun | 2009 | Journal of Computational and Applied Mathematics2009，230，10： | 1 |
| 13 | Velocity bunching for the linac of Shanghai Deep UV FEL facility显示文摘High brightness electron beam is one of the main goals of the research and development effort in RF photo-injectors.Compared with the normally used magnetic chicane,an alternative scheme,commonly known as 'velocity bunching',has been proposed as a tool to compress electron beam pulses in modern high brightness photo-injector sources.This paper presents numerical optimization systematically and the first attempt to demonstrate the velocity bunching scheme on SDUV-FEL linac experimentally.The relationship between the degree of bunching and the off-crest phase of the accelerating structure is explored experimentally.Velocity bunching operating mode illustrates flexible performances with an intrinsic machine jitter,which agrees well with the theoretical prediction. | ZHANG Meng GU Duan GU Qiang LIN Guoqiang LAN Taihe WANG Xingtao DENG Haixiao | 2012 | Nuclear Science and Techniques2012，23，3： | 1 |
| 14 | Texture segmentation based on Zernike moment and BP neural network 显示文摘 | LI Haixiao JIANG Lu SHU Huazhong | 2005 | Journal of Southeast University2005，35，2： | 1 |
| 15 | Intermodal transfer between bicy- cles and rail transit in Shanghai, China显示文摘 | PAN Haixiao Shen Qing Xue Song | 2010 | Journal of Transportation Re- search Record2010，2144，： | 1 |
| 16 | Influence of Urban Form on Travel Behaviour in Four Neighbourhoods of shanghai显示文摘 | Pan Haixiao Shen Qing Zhang Ming | 2009 | Urban Study2009，，46： | 1 |
| 17 | The review of experience, challenge and expectation显示文摘 | PAN Haixiao REN Chunyang | 2004 | Urban Planning Internation- al2004，19，6： | 1 |
| 18 | Influence of urban form on travel bebaviour in four neighbourhoods of Shanghai显示文摘 | PAN Haixiao SHEN Qing ZHANG Ming | 2009 | Urban Studies2009，46，2： | 1 |
| 19 | Travel and car ownership of residents near new suburban metro stations in Shanghai显示文摘 | PAN Haixiao SHEN Qing ZHAO Ting | 2013 | Transportation Research Record2013，，12： | 1 |
| 20 | Confocal Laser Scanning Microscope Evaluation of Early Bacterial Colonization on Zirconium Oxide and Titanium Surfaces:An in vivo Study显示文摘To investigate the bacterial colonization on zirconium oxide and titanium surfaces in vivo quantitatively using a confocal laser scanning microscope (CLSM). Ten samples of zirconium oxide ceramic and commercially pure titanium were fabricated and polished using silicon carbide abrasive paper. One sample from each group was evaluated topographic pattern under a scanning electron microscope. One sample from each group was to evaluate roughness using a profilometer. Eight volunteers were selected. The samples were cemented at the buccal surfaces of upper first molars. All samples were removed after 48 hours, immersed in SYTO-9 and propidium iodide fluorescent to stain for adherent bacteria and observed with CLSM. Fewer bacteria were observed in zirconia group than titanium group. However, there was no statistical difference between two groups. The experimental results demonstrate that zirconium oxide may be considered as a promising material for dental implant abutments. | 王敏 白轶 YANG Hui ZOU Haixiao 夏海斌 王贻宁 | 2013 | Journal of Wuhan University of Technology(Materials Science)2013，28，2： | 1 |
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