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| 1 | Parallel selection of distinct Tof5 alleles drove the adaptation of cultivated and wild soybean to high latitudes显示文摘Photoperiod responsiveness is a key factor limiting the geographic distribution of cultivated soybean and its wild ancestor.In particular,the genetic basis of the adaptation in wild soybean remains poorly understood.In this study,by combining whole-genome resequencing and genome-wide association studies we identified a novel locus,Time of Flowering 5(Tof5),which promotes flowering and enhances adaptation to high latitudes in both wild and cultivated soybean.By genomic,genetic and transgenic analyses we showed that Tof5 en-codes a homolog of Arabidopsis thaliana FRUITFULL(FUL).Importantly,further analyses suggested that different alleles of Tof5 have undergone parallel selection.The Tof5H1 allele was strongly selected by humans after the early domestication of cultivated soybean,while Tof5H2 allele was naturally selected in wild soybean,and in each case facilitating adaptation to high latitudes.Moreover,we found that the key flowering repressor E1 suppresses the transcription of Tof5 by binding to its promoter.In turn,Tof5 physically associates with the promoters of two important FLOWERING LOCUS T(FT),FT2a and FT5a,to upregulate their transcription and promote flowering under long photoperiods.Collectively,ourfindings provide insights into how wild soybean adapted to high latitudes through natural selection and indicate that cultivated soybean underwent changes in the same gene but evolved a distinct allele that was artificially selected after domestication. | Lidong Dong Qun Cheng Chao Fang Lingping Kong Hui Yang Zhihong Hou Yongli Li Haiyang Nan Yuhang Zhang Qingshan Chen Chunbao Zhang Kun Kou Tong Su Lingshuang Wang Shichen Li Haiyang Li Xiaoya Lin Yang Tang Xiaohui Zhao Sijia Lu Baohui Liu Fanjiang Kong | 2022 | Molecular Plant2022，15，2： | 5 |
| 2 | Reductive amination of 1,6-hexanediol with Ru/Al2O3 catalyst in supercritical ammonia显示文摘Hexamethylenediamine(HMDA) is an important reagent for the synthesis of Nylon-6,6, and it is usually produced by the hydrogenation of adiponitrile using a toxic reagent of hydrocyanic acid. Herein, we developed an environmental friendly route to produce HMDA via catalytic reductive amination of 1,6-hexanediol(HDO) in the presence of hydrogen. The activities of several heterogeneous metal catalysts such as supported Ni, Co, Ru, Pt, Pd catalysts were screened for the present reaction in supercritical ammonia without any additives. Among the catalysts examined, Ru/Al_2O_3 presented a high catalytic activity and highest selectivity for the desired product of HMDA. The high performance of Ru/Al_2O_3 was discussed based on the Ru dispersion and the surface properties like the acid-basicity. In addition, the reaction parameters such as reaction temperature,time, H_2 and NH_3 pressure were examined, and the reaction processes were discussed in detail. | Yan Li Haiyang Cheng Chao Zhang Bin Zhang Tong Liu Qifan Wu Xinluona Su Weiwei Lin Fengyu Zhao | 2017 | Science China Chemistry2017，60，7： | 5 |
| 3 | Two soybean homologues of TERMINAL FLOWER 1 control flowering time under long day conditions显示文摘Flowering time is a key agronomic trait that directly affect the adaptation and yield of soybean.After whole genome duplications,about 75%of genes being represented by multiple copies in soybean.There are four TERMINAL FLOWER 1(TFL1)genes in soybean,and the TFL1b(Dt1)has been characterized as the determinant of stem growth habit.The function of other TFL1 homologs in soybean is still unclear.Here,we generated knockout mutants by CRISPR/Cas9 genome editing technology and found that the tfl1c/tfl1d double mutants flowered significantly earlier than wild-type plants.We investigated that TFL1c and TFL1d could physically interact with the b ZIP transcription factor FDc1 and bind to the promoter of APETALA1a(AP1a).RNA-seq and q RT-PCR analyses indicated that TFL1c and TFL1d repressed the expressions of the four AP1 homologs and delayed the flowering time in soybean.The two genes play important roles in the regulation of flowering time in soybean and mainly act as the flowering inhibitors under long-day conditions.Our results identify novel components in the flowering-time regulation network of soybean and will be invaluable for molecular breeding of improved soybean yield. | Lingshuang Wang Chun Lin Bohui Li Tong Su Shichen Li Haiyang Li Fanglei He Chuanjie Gou Zheng Chen Yanan Wang Jun Qin Baohui Liu Fanjiang Kong Lin Yue Sijia Lu Chao Fang | 2023 | The Crop Journal2023，11，3： | 3 |
| 4 | Development and validation of InDel markers for identification of QTL underlying flowering time in soybean显示文摘Soybean [Glycine max(L.) Merrill] is a major plant source of protein and oil. An accurate and well-saturated molecular linkage map is a prerequisite for forward genetic studies of gene function and for modern breeding for many useful agronomic traits. Next-generation sequence data available in public databases provides valuable information and offers new insights for rapid and efficient development of molecular markers. In this study, we attempted to show the feasibility and facility of using genomic resequencing data as raw material for identifying putative In Del markers. First, we identified 17,613 In Del sites among 56 soybean accessions and obtained 12,619 primer pairs. Second, we constructed a genetic map with a random subset of 2841 primer pairs and aligned 300 polymorphic markers with the 20 consensus linkage groups(LG). The total genetic distance was 2347.3 c M and the number of mapped markers per LG ranged from 10 to 23 with an average of 15 markers. The largest and smallest genetic distances between adjacent markers were 52.3 c M and 0.1 cM, respectively. Finally, we validated the genetic map constructed by newly developed In Del markers by QTL analysis of days to flowering(DTF) under different environments. One major QTL(qDTF4) and four minor QTL(qDTF20, qDTF13, qDTF12,and q DTF11) on 5 LGs were detected. These results demonstrate the utility of the In Del markers developed in this work for map-based cloning and molecular breeding in soybean. | Jialin Wang Lingping Kong Kanchao Yu Fengge Zhang Xinyi Shi Yanping Wang Haiyang Nan Xiaohui Zhao Sijia Lu Dong Cao Xiaoming Li Chao Fang Feifei Wang Tong Su Shichen Li Xiaohui Yuan Baohui Liu Fanjiang Kong | 2018 | The Crop Journal2018，6，2： | 1 |
| 5 | Genetic mapping of folate QTLs using a segregated population in maizeFA显示文摘As essential B vitamin for humans, folates accumulation in edible parts of crops, such as maize kernels, Is of great importance for human health. But its breeding is always limited by the prohibitive cost of folate profiling. The molecular breeding is a more executable and efficient way for folate fortification, but is limited by the molecular knowledge of folate regulation. Here we report the genetic mapping of folate quantitative trait loci (QTLs) using a segregated population crossed by two maize lines, one high in folate (GEMS31) and the other low in folate (DAN3130). Two folate QTLs on chromosome 5 were obtained by the combination of F2 whole-exome sequencing and F3 kernel-folate profiling. These two QTLs had been confirmed by bulk segregant analysis using F6 pooled DNA and F7 kernel-folate profiling, and were overlapped with QTLs identified by another segregated population. These two QTLs contributed 41.6% of phenotypic variation of 5-formyltetrahydrofolate, the most abundant storage form among folate derivatives in dry maize grains, in the GEMS31×DAN3130 population. Their fine mapping and functional analysis will reveal details of folate metabolism, and provide a basis for marker-assisted breeding aimed at the enrichment of folates in maize kernels. | Wenzhu Guo Tong Lian Baobao Wang Jiantao Guan Dong Yuan Huan Wang Fardous Mohammad Safiul Azam Xing Wan Weixuan Wang Qiuju Liang Haiyang Wang Jinxing Tu Chunyi Zhang Ling Jiang | 2019 | Journal of Integrative Plant Biology2019，61，6： | 1 |
| 6 | Biodegradable,superhydrophobic walnut wood membrane for the separation of oil/water mixtures显示文摘The preparation of environmentally friendly oil/water separation materials remains a great challenge.Freeze-drying of wood after lignin removal yields wood aerogels,which can be used as substrates to prepare fluorine-free environmentally friendly superhydrophobic materials,However,they are more suitable for absorption rather than filtration applications due to their poor strength.A study using cross-sections of pristine wood chips as substrates retains the original strength of wood,but the use of the cross-sectional of wood pieces limits their thickness,strength,and size.In this paper,a degradable fluorine-free superhydrophobic film(max.water contact angle of approximately 164.2°)with self-cleaning and abrasion resistance characteristics was prepared by a one-step method using pristine and activated walnut longitudinal section films as the substrate,with tetraethyl orthosilicate as a precursor and dodecyltriethoxysilane as a modifier.The tensile strength results show that superhydrophobic films with pristine or activated wood substrates maintained the strength of pristine wood and were 2.2 times stronger than the wood aerogel substrate.In addition,after cross-laminating the two samples,the films had the ability to separate oil and water by continuous filtration with high efficiency(98.5%)and flux(approximately 1.3×10^(3)L∙m^(‒2)∙h^(‒1)).The method has potential for the large-scale fabrication of degradable superhydrophobic filtration separation membranes. | Tong Xing Changqing Dong Xiaodong Wang Xiaoying Hu Changrui Liu Haiyang Lv | 2022 | Frontiers of Chemical Science and Engineering2022，16，9： | 0 |
| 7 | Single-dose AAV-based vaccine induces a high level of neutralizing antibodies against SARS-CoV-2 in rhesus macaques显示文摘Dear Editor,Coronavirus disease 2019(COVID-19)is a highly infectious respiratory disease that continues to pose a serious global public health emergency.The disease shows a high infection rate,long incubation period,and rapidly emerging variants,which have led to its rapid spread worldwide(Krammer 2020).Many vaccines have been developed for the control of severe acute respiratory syndrome coronavirus 2(SARS-CoV-2),the virus responsible for COVID-19,including vaccines based on messenger RNA(mRNA)(Polack et al.2020),viral vectors(Zhu et al.2020),recombinant proteins(Yang et al.2020),and inactivated SARS-CoV-2(Zhang et al.2021). | Dali Tong Mei Zhang Yunru Yang Han Xia Haiyang Tong Huajun Zhang Weihong Zeng Muziying Liu Yan Wu Huan Ma Xue Hu Weiyong Liu Yuan Cai Yanfeng Yao Yichuan Yao Kunpeng Liu Shifang Shan Yajuan Li Ge Gao Weiwei Guo Yun Peng Shaohong Chen Juhong Rao Jiaxuan Zhao Juan Min Qingjun Zhu Yanmin Zheng Lianxin Liu Chao Shan Kai Zhong Zilong Qiu Tengchuan Jin Sandra Chiu Zhiming Yuan Tian Xue | 2023 | Protein & Cell2023，14，1： | 0 |
| 8 | 3-dose of RBD vaccine is sufficient to elicit a long-lasting memory response against SARS-CoV-2 infection显示文摘Dear Editor,The spread of severe acute respiratory syndrome coronavirus 2(SARS-CoV-2)led to a global pandemic with 260 million infected people.A variety of vaccines,including the mRNA and recombinant vaccines,were developed to prevent the spread of the disease.1,2 There is an urgent need for testing the vaccine-induced long-term memory response based on the immunological principles and identifying the optimal combination of dose and injection manner to provide important information for clinical application. | Mengqing Cong Yunru Yang Haiyang Tong Ajmeri Sultana Shimu Baolong Wang Qing Li Fengyin Li Yi Yang Tengchuan Jin Bofeng Li | 2022 | Signal Transduction and Targeted Therapy2022，7，4： | 0 |
| 9 | Unraveling the synergetic mechanism of physisorption and chemisorption in laser-irradiated monolayer WS_(2)显示文摘To further improve the quantum efficiency of atomically thin transition metal dichalcogenides (TMDs) is crucial for the realization of high-performance optoelectronic applications. To this regard, a few chemical or physical approaches such as superacid treatment, electrical gating, dielectric screening, and laser irradiation have been developed. In particular, the laser irradiation appears to be a more efficient way with good processability and spatial selectivity. However, the underlying mechanism especially about whether chemisorption or physisorption plays a more important role is still debatable. Here, we unravel the mystery of laser irradiation induced photoluminescence enhancement in monolayer WS_(2) by precisely controlling irradiation time and environment. It is found that the synergetic effect of physisorption and chemisorption is responsible for the photoluminescence enhancement, where the physisorption dominates with more than 74% contribution. The comprehensive understanding of the adsorption mechanism in laser-irradiated TMDs may trigger the potential applications for patterned light source, effective photosensor and ultrathin optical memory. | Yuanzheng Li Jiaxu Yan Jinping Chen Tong Yu Hang Ren Xiuling Liu Weizhen Liu Guochun Yang Chunxiang Xu Qiaoliang Bao Yichun Liu Haiyang Xu | 2021 | Nano Research2021，14，11： | 0 |
| 10 | Scalable synthesis of lipid nanoparticles for nucleic acid drug delivery using an isometric channel-size enlarging strategy显示文摘Lipid nanoparticles(LNPs)have emerged as highly effective delivery systems for nucleic acid-based therapeutics.However,the broad clinical translation of LNP-based drugs is hampered by the lack of robust and scalable synthesis techniques that can consistently produce formulations from early development to clinical application.In this work,we proposed a method to achieve scalable synthesis of LNPs by scaling inertial microfluidic mixers isometrically in three dimensions.Moreover,a theoretical predictive method,which controls the mixing time to be equal across different chips,is developed to ensure consistent particle size and size distribution of the synthesized LNPs.LNPs loaded with small interfering RNA(siRNA)were synthesized at different flow rates,exhibiting consistent physical properties,including particle size,size distribution and encapsulation efficiency.This work provides a practical approach for scalable synthesis of LNPs consistently,offering the potential to accelerate the transition of nucleic acid drug development into clinical application. | Zesen Ma Haiyang Tong Sijin Lin Li Zhou Demeng Sun Baoqing Li Changlin Tian Jiaru Chu | 2024 | Nano Research2024，17，4： | 0 |
| 11 | Technical Measures for Rapid Propagation of Improved Beef Cattle显示文摘Based on early weaning in calves,hormone induction in young calves,intravital egg collection,in-vitro fertilization and embryo transfer which serve as major breakthroughs in livestock breeding technology,the techniques of hormone induction and in-vitro fertilization were integrated,not only to solve the problem of obtaining oocytes by hormone induction in adult cows in production practice,but also to obviously improve production efficiency.The application of this technology can help accelerate the cultivation process,shorten the generation interval,promote the industrialization and improve the economic benefits of beef cattle breed.This paper provides a reference for the research and application of new breeding and propagation techniques of beef cattle. | Caihong HAO Guizhi TONG Xueying SONG Xinying DING Likun WANG Zhenhua JIN Xintao LI Haiyang YOU Guowang CHEN Jingdong NAN Jiansheng ZHANG | 2022 | Agricultural Biotechnology2022，11，6： | 0 |
| 12 | Multiplex CRISPR/Cas9-mediated knockout of soybean LNK2 advances flowering time显示文摘Flowering time is an important agronomic trait for soybean yield and adaptation. However, the genetic basis of soybean adaptation to diverse latitudes is still not clear. Four NIGHT LIGHT-INDUCIBLE AND CLOCK-REGULATED 2(LNK2) homeologs of Arabidopsis thaliana LNK2 were identified in soybean. Three single-guide RNAs were designed for editing the four LNK2 genes. A transgene-free homozygous quadruple mutant of the LNK2 genes was developed using the CRISPR(clustered regularly interspaced short palindromic repeats)/Cas9(CRISPR-associated protein 9). Under long-day(LD) conditions, the quadruple mutant flowered significantly earlier than the wild-type(WT). Quantitative real-time PCR(q RT-PCR)revealed that transcript levels of LNK2 were significantly lower in the quadruple mutant than in the WT under LD conditions. LNK2 promoted the expression of the legume-specific E1 gene and repressed the expression of FT2 a. Genetic markers were developed to identify LNK2 mutants for soybean breeding.These results indicate that CRISPR/Cas9-mediated targeted mutagenesis of four LNK2 genes shortens flowering time in soybean. Our findings identify novel components in flowering-time control in soybean and may be beneficial for further soybean breeding in high-latitude environments. | Zhaobo Li Qun Cheng Zhuoran Gan Zhihong Hou Yuhang Zhang Yongli Li Haiyang Li Haiyang Nan Cen Yang Linnan Chen Sijia Lu Wenqian Shi Liyu Chen Yanping Wang Chao Fang Liping Kong Tong Su Shichen Li Kun Kou Lingshuang Wang Fanjiang Kong Baohui Liu Lidong Dong | 2021 | The Crop Journal2021，9，4： | 0 |
| 13 | A recent retrotransposon insertion of J caused E6 locus facilitating soybean adaptation into low latitude显示文摘Soybean(Glycine max) is an important legume crop that was domesticated in temperate regions.Soybean varieties from these regions generally mature early and exhibit extremely low yield when grown under inductive short-day(SD) conditions at low latitudes. The long-juvenile(LJ) trait, which is characterized by delayed flowering and maturity,and improved yield under SD conditions, allowed the cultivation of soybean to expand to lower latitudes. Two major loci control the LJ trait: J and E6. In the current study, positional cloning, sequence analysis, and transgenic complementation confirmed that E6 is a novel allele of J, the ortholog of Arabidopsis thaliana EARLY FLOWERING 3(ELF3). The mutant allele e6^(PG), which carries a Ty1/Copia-like retrotransposon insertion, does not suppress the legume-specific flowering repressor E1, allowing E1 to inhibit Flowering Locus T(FT) expression and thus delaying flowering and increasing yields under SD conditions. The e6^(PG)allele is a rare allele that has not been incorporated into modern breeding programs.The dysfunction of J might have greatly facilitated the adaptation of soybean to low latitudes. Our findings increase our understanding of the molecular mechanisms underlying the LJ trait and provide valuable resources for soybean breeding. | Chao Fang Jun Liu Ting Zhang Tong Su Shichen Li Qun Cheng Lingping Kong Xiaoming Li Tiantian Bu Haiyang Li Lidong Dong Sijia Lu Fanjiang Kong Baohui Liu | 2021 | Journal of Integrative Plant Biology2021，63，6： | 0 |
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