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| 1 | In Situ 3D Bioprinting Living Photosynthetic Scaffolds for Autotrophic Wound Healing显示文摘Three-dimensional(3D)bioprinting has been extensively explored for tissue repair and regeneration,while the insufficient nutrient and oxygen availability in the printed constructs,as well as the lack of adaptive dimensions and shapes,compromises the overall therapeutic efficacy and limits their further application.Herein,inspired by the natural symbiotic relationship between salamanders and algae,we present novel living photosynthetic scaffolds by using an in situ microfluidic-assisted 3D bioprinting strategy for adapting irregular-shaped wounds and promoting their healing.As the oxygenic photosynthesis unicellular microalga(Chlorella pyrenoidosa)was incorporated during 3D printing,the generated scaffolds could produce sustainable oxygen under light illumination,which facilitated the cell proliferation,migration,and differentiation even in hypoxic conditions.Thus,when the living microalgae-laden scaffolds were directly printed into diabetic wounds,they could significantly accelerate the chronic wound closure by alleviating local hypoxia,increasing angiogenesis,and promoting extracellular matrix(ECM)synthesis.These results indicate that the in situ bioprinting of living photosynthetic microalgae offers an effective autotrophic biosystem for promoting wound healing,suggesting a promising therapeutic strategy for diverse tissue engineering applications. | Xiaocheng Wang Chaoyu Yang Yunru Yu Yuanjin Zhao | 2022 | Research2022，，3： | 1 |
| 2 | Bio-inspired dual-adhesive particles from microfluidic electrospray for bone regeneration显示文摘Bioadhesive hydrogels have demonstrated great potential in bone regeneration.However,the relatively simple adhesion mechanism and lack of intricate structural design restrict their further applications.Herein,inspired by multiple adhesion mechanisms of pollen particles and marine mussels,we present a novel type of dual-adhesive hydrogel particles fabricated from microfluidic electrospray for bone regeneration.As the particles are rapidly solidified via liquid nitrogen-assisted cryogelation,they exhibit pollen-mimicking hierarchical porous morphology and gain structure-related adhesion.Besides,the particles are further coated by polydopamine(PDA)to achieve molecular-level adhesion especially to physiological wet surfaces of bone issues.Benefiting from such dual-adhesion mechanisms,the particles can strongly adhere to bone tissue defects,and function as porous scaffolds.Moreover,the dual-adhesive particles can serve as effective vehicles to release key growth factors more than two weeks.In vitro experiments showed that the growth factors-loaden particles have excellent biocompatibility and more significantly promote angiogenesis(~2-fold)and osteogenic differentiation(~3-fold)than control.In vivo experiments indicated that the dual-adhesive particles could significantly enhance bone regeneration(~4-fold)than control by coupling osteogenesis and angiogenesis effects.Based on these features,the bio-inspired dual-adhesive particles have great potentials for bone repair and wound healing applications. | Lei Yang Xiaocheng Wang Yunru Yu Luoran Shang Wei Xu Yuanjin Zhao | 2023 | Nano Research2023，16，4： | 1 |
| 3 | Hetero-bivalent nanobodies provide broad-spectrum protection against SARS-CoV-2 variants of concern including Omicron显示文摘SARS-CoV-2 variants with adaptive mutations have continued to emerge,causing fresh waves of infection even amongst vaccinated population.The development of broad-spectrum antivirals is thus urgently needed.We previously developed two hetero-bivalent nanobodies(Nbs),aRBD-2-5 and aRBD-2-7,with potent neutralization activity against the wild-type(WT)Wuhan isolated SARS-CoV-2,by fusing aRBD-2 with aRBD-5 and aRBD-7,respectively.Here,we resolved the crystal structures of these Nbs in complex with the receptor-binding domain(RBD)of the spike protein,and found that aRBD-2 contacts with highly conserved RBD residues and retains binding to the RBD of the Alpha,Beta,Gamma,Delta,Delta plus,Kappa,Lambda,Omicron BA.1,and BA.2 variants.In contrast,aRBD-5 and aRBD-7 bind to less conserved RBD epitopes non overlapping with the epitope of aRBD 2,and do not show apparent binding to the RBD of some variants.However,when fused with aRBD-2,they effectively enhance the overal binding affinity.Consistently,aRBD-2-5-Fc and aRBD-2-7 Fc potently neutralized all of the tested authentic or pseudotyped viruses,incuding WT,Alpha,Beta,Gamma,Delta,and Omicron BA.1,BA.1.1 and BA.2.Furthermore,aRBD-2-5-FC provided prophylactic protection against the WT and mouse-adapted SARS CoV-2 in mice,and conferred protection against the Omicron BA.1 variant in hamsters prophylatically and therapeutically,indicating that aRBD-2-5-Fc could potentially beneft the prevention and treatment of COVID-19 caused by the emerging variants of concern.Our strategy provides new solutions in the development of broad-spectrum therapeutic antibodies for COVID-19. | Huan Ma Xinghai Zhang Peiyi Zheng Peter H.Dube Weihong Zeng Shaohong Chen Qingyu Cheng Yunru Yang Yan Wu Junhui Zhou Xiaowen Hu Yan Xiang Huajun Zhang Sandra Chiu Tengchuan Jin | 2022 | Cell Research2022，32，9： | 1 |
| 4 | Shear-flow-induced graphene coating microfibers from microfluidic spinning显示文摘The advancements in flexible electronics call for invention of fiber-based electronic systems by surface modification or encapsulation.Here we present novel shear-flow-induced graphene nanosheets coating microfibers by integrating the dip coating approach with the microfluidic spinning method.The core hydrogel microfiber was first spun continuously from the microfluidic device,and the shear flow from the dip coating approach allowed formation of the thin graphene oxide(GO)nanosheet coating shell. | Yunru Yu Jiahui Guo Han Zhang Xiaocheng Wang Chaoyu Yang Yuanjin Zhao | 2022 | The Innovation2022，3，2： | 1 |
| 5 | Single-dose AAV-based vaccine induces a high level of neutralizing antibodies against SARS-CoV-2 in rhesus macaques显示文摘Dear Editor,Coronavirus disease 2019(COVID-19)is a highly infectious respiratory disease that continues to pose a serious global public health emergency.The disease shows a high infection rate,long incubation period,and rapidly emerging variants,which have led to its rapid spread worldwide(Krammer 2020).Many vaccines have been developed for the control of severe acute respiratory syndrome coronavirus 2(SARS-CoV-2),the virus responsible for COVID-19,including vaccines based on messenger RNA(mRNA)(Polack et al.2020),viral vectors(Zhu et al.2020),recombinant proteins(Yang et al.2020),and inactivated SARS-CoV-2(Zhang et al.2021). | Dali Tong Mei Zhang Yunru Yang Han Xia Haiyang Tong Huajun Zhang Weihong Zeng Muziying Liu Yan Wu Huan Ma Xue Hu Weiyong Liu Yuan Cai Yanfeng Yao Yichuan Yao Kunpeng Liu Shifang Shan Yajuan Li Ge Gao Weiwei Guo Yun Peng Shaohong Chen Juhong Rao Jiaxuan Zhao Juan Min Qingjun Zhu Yanmin Zheng Lianxin Liu Chao Shan Kai Zhong Zilong Qiu Tengchuan Jin Sandra Chiu Zhiming Yuan Tian Xue | 2023 | Protein & Cell2023，14，1： | 0 |
| 6 | Cuticular compounds inhibit cannibalism of early-instar spiderlings by pulli-carrying Pardosa pseudoannulata females显示文摘Cannibalism is common in spiders.Wolf spider(Lycosidae)females,which exhibit extensive maternal care,have been reported to cannibalize less when they are carrying egg sacs and juveniles.In a laboratory experiment,we demonstrated that cannibalism of early-instar spiderlings(EIS)by a wolf spider(Pardosa pseudoannulata)mother was almost completely inhibited when she was carrying spiderlings.Compared with virgin and mated-females,mother spiders tolerated more and predated fewer spiderlings,including gregarious pulli and newly dispersed spiderlings(NDS).Cannibalism of EIS by females during their reproductive period exhibited a V-shaped pattern,with a gradual decrease from the egg sac-carrying to pulli-carrying(PC)stage,and a recovery from the PC stage to post-reproductive(PR)stage.Notably,there was 0 cannibalism at the PC stage.PC females exhibited no interest in pulli,while PR females were attracted to and predated pulli and NDS as they did their natural prey,Nilaparvata lugens.Interestingly,PC females captured and released NDS in a foraging assay,although attraction was observed from olfactometer measurements.PC mothers possessed a cuticular volatile profile that was closer to that of pulli and NDS than to that of PR females.Moreover,NDS cuticular extract provoked an electrophysiological response in legs of PC females.Therefore,cuticular compound-mediated chemical communication may be involved in inhibiting cannibalism of EIS by spider mothers,and especially in eliminating cannibalism by PC mothers.Future studies will aim to characterize the specific cuticular compounds and chemoreception mechanism in females,which will facilitate our understanding of intraspecific recognition and cannibalism in spiders. | Na Yu Yunru Chen Guangming Xu Zhiming Yang Shijie Wang Tianyu Lu Yixi Zhang Zewen Liu | 2022 | Insect Science2022，29，5： | 0 |
| 7 | Smooth muscle AKG/OXGR1 signaling regulates epididymal fluid acid–base balance and sperm maturation显示文摘Infertility is a global concern attributed to genetic defects,lifestyle,nutrition,and any other factors that affect the local metabolism and niche microenvironment of the reproductive system.2-Oxoglutarate receptor 1(OXGR1)is abundantly expressed in the testis;however,its cellular distribution and biological function of OXGR1 in the male reproductive system remain unclear.In the current study,we demonstrated that OXGR1 is primarily expressed in epididymal smooth muscle cells(SMCs).Aging and heat stress significantly reduced OXGR1 expression in the epididymis.Using OXGR1 global knockout and epididymal-specific OXGR1 knockdown models,we revealed that OXGR1 is essential for epididymal sperm maturation and fluid acid–base balance.Supplementation ofα-ketoglutaric acid(AKG),the endogenous ligand of OXGR1,effectively reversed epididymal sperm maturation disorders caused by aging and heat stress.Furthermore,in vitro studies showed that AKG markedly stimulated the release of instantaneous intracellular calcium from epididymal SMCs and substantially reduced the pH_(i) value in the epididymal SMCs via OXGR1.Mechanistically,we discovered that AKG/OXGR1 considerably increased the expression of Na^(+)/HCO_(3)^(−)cotransporter(NBCe1)mRNA in the epididymal SMCs,mediated by intracellular calcium signaling.The local AKG/OXGR1 system changed the epididymal fluid pH value and HCO_(3)^(−)concentration,thereby regulating sperm maturation via intracellular calcium signaling and NBCe1 mRNA expression.This study for the first time reveals the crucial role of OXGR1 in male fertility and sheds light on the applicability of metabolic intermediates in the nutritional intervention of reproduction. | Chang Xu Yexian Yuan Cha Zhang Yuchuan Zhou Jinping Yang Huadong Yi Ishwari Gyawali Jingyi Lu Sile Guo Yunru Ji Chengquan Tan Songbo Wang Yongliang Zhang Qingyan Jiang Gang Shu | 2022 | Life Metabolism2022，1，1： | 0 |
| 8 | Hertz-rate metropolitan quantum teleportation显示文摘Quantum teleportation can transfer an unknown quantum state between distant quantum nodes,which holds great promise in enabling large-scale quantum networks.To advance the full potential of quantum teleportation,quantum states must be faithfully transferred at a high rate over long distance.Despite recent impressive advances,a high-rate quantum teleportation system across metropolitan fiber networks is extremely desired.Here,we demonstrate a quantum teleportation system which transfers quantum states carried by independent photons at a rate of 7.1±0.4 Hz over 64-km-long fiber channel.An average single-photon fidelity of≥90.6±2.6%is achieved,which exceeds the maximum fidelity of 2/3 in classical regime.Our result marks an important milestone towards quantum networks and opens the door to exploring quantum entanglement based informatic applications for the future quantum internet. | Si Shen Chenzhi Yuan Zichang Zhang Hao Yu Ruiming Zhang Chuanrong Yang Hao Li Zhen Wang You Wang Guangwei Deng Haizhi Song Lixing You Yunru Fan Guangcan Guo Qiang Zhou | 2023 | Light(Science & Applications)2023，12，6： | 0 |
| 9 | Bioinspired Jellyfish Microparticles from Microfluidics显示文摘Nonspherical particles have attracted increasing interest because of their shape anisotropy.However,the current methods to prepare anisotropic particles suffer from complex generation processes and limited shape diversity.Here,we develop a piezoelectric microfluidic system to generate complex flow configurations and fabricatejellyfish-like microparticles.In this delicate system,the piezoelectric vibration could evolve a jellyfish-like flow configuration in the microchannel and the in situ photopolymerization could instantly capture the flow architecture.The sizes and morphologies of the particles are precisely controlled by tuning the piezoelectric and microfluidic parameters.Furthermore,multi-compartmental microparticles with a dual-layer structure are achieved by modifying the injecting channel geometry.Moreover,such unique a shape endows the particles with flexible motion ability especially when stimuliresponsive materials are incorporated.On the basis of that,we demonstrate the capability of the jellyfish-like microparticles in highly efficient adsorption of organic pollutants under external control.Thus,it is believed that such jellyfish-like microparticles are highly versatile in potential applications and the piezoelectricintegrated microfluidic strategy could open an avenue for the creation of such anisotropic particles. | Chaoyu Yang Yunru Yu Yuaijin Zhao Luoran Shang | 2023 | Research2023，，1： | 0 |
| 10 | 3-dose of RBD vaccine is sufficient to elicit a long-lasting memory response against SARS-CoV-2 infection显示文摘Dear Editor,The spread of severe acute respiratory syndrome coronavirus 2(SARS-CoV-2)led to a global pandemic with 260 million infected people.A variety of vaccines,including the mRNA and recombinant vaccines,were developed to prevent the spread of the disease.1,2 There is an urgent need for testing the vaccine-induced long-term memory response based on the immunological principles and identifying the optimal combination of dose and injection manner to provide important information for clinical application. | Mengqing Cong Yunru Yang Haiyang Tong Ajmeri Sultana Shimu Baolong Wang Qing Li Fengyin Li Yi Yang Tengchuan Jin Bofeng Li | 2022 | Signal Transduction and Targeted Therapy2022，7，4： | 0 |
| 11 | Multifunctional GO Hybrid Hydrogel Scaffolds for Wound Healing显示文摘Hydrogel dressings have received extensive attention for the skin wound repair,while it is still a challenge to develop a smart hydrogel for adapting the dynamic wound healing process.Herein,we develop a novel graphene oxide(GO)hybrid hydrogel scaffold with adjustable mechanical properties,controllable drug release,and antibacterial behavior for promoting wound healing. | Xiaoya Ding Yunru Yu Chaoyu Yang Dan Wu Yuanjin Zhao | 2023 | Research2023，，2： | 0 |
| 12 | Characterization of SARS-CoV-2-specific antibodies in COVID-19 patients reveals highly potent neutralizing IgA显示文摘Dear Editor,The coronavirus disease 2019(COVID-19),caused by the Severe Acute Respiratory Syndrome Coronavirus 2(SARS-CoV-2),is an ongoing threat to public health.Due to the presence of neutralizing antibodies in convalescent patients,convalescent plasma transfusion has been tested as a promising therapy for severe COVID-19 patients.12 We and others previously found a surprisingly early serum lgA antibody response in the COVID-19 patients.However,the relative contribution of the lgA antibody towards virus neutralization with respect to other antibody isotypes is unknown. | Weihong Zeng Huan Ma Chengchao Ding Yunru Yang Yong Sun Xiaoxue Huang Weihuang He Yan Xiang Yong Gao Tengchuan Jin | 2021 | Signal Transduction and Targeted Therapy2021，6，2： | 0 |
| 13 | Microtubes with gradient decellularized porcine sciatic nerve matrix from microfluidics for sciatic nerve regeneration显示文摘Long-range peripheral nerve defect is a severe and worldwide disease.With the increasing development of tissue engineering,the excellent ability of nerve extracellular matrix(ECM)in peripheral nerve injury(PNI)has been widely studied and verified.Here,we present a novel microtube that contains gradient decellularized porcine sciatic nerve ECM hydrogel(pDScNM-gel)from microfluidics for sciatic nerve regeneration.The pDScNM is confirmed to enhance cell proliferation and migration,and improve the axon growth of primary dorsal root ganglions(DRGs)in a concentration-related manner.These behaviors were also achieved when cells were co-cultured in a gradient pDScNM microtube.The in vivo sciatic nerve regeneration and functional recovery were also demonstrated by assembling the gradient pDScNM microtubes with a medical silicon tube.These results indicated that the microtubes with gradient pDScNM could act as a promising alternative for repairing peripheral nerve defects and showed great potential in clinical use. | Binghui Jin Yunru Yu Xiangxiang Chen Yanhong Yang Yushan Xiong Young Jun Im Yuanjin Zhao Jian Xiao | 2023 | Bioactive Materials2023，，3： | 0 |
| 14 | Cellular fluidic-based vascular networks for tissue engineering显示文摘Artificial organs are devices implanted into the living body as a substitute for damaged or diseased organs.Current efforts focus on the construction of fully functionalized artificial tissues/organs with vascular networks.Although engineering efforts have been made in creating artificial vessels with simple or complex configurations,building vascular networks with hierarchical architectures approximating native counterparts remains challenging.Herein,we give a perspective of cellular fluidics-based construction of vascular networks for tissue engineering,with inspirations drawn from a novel concept of 3D fluidic control platform based on unit-cell constructs.Through architected design of the unit cells,it enables programmed control over gas-liquid-solid interfaces and fluid flow processes in open-cell structures.This cellular-fluidics concept and the associated platform provide lots of inspirations for constructing artificial vascular networks.We believe that cellular fluidics opens a new avenue for fluid control and deterministic delivery,and would find vast opportunities in tissue engineering. | Chaoyu Yang Yunru Yu Xiaocheng Wang Qiao Wang Luoran Shang | 2021 | Engineered Regeneration2021，2，1： | 0 |
| 15 | Decline of SARS-CoV-2-specific IgG,IgM and IgA in convalescent COVID-19 patients within 100 days after hospital discharge显示文摘Dear editor,The 2019 novel coronavirus(later renamed as SARS-CoV-2 in February 2020)infected over 12 million people globally by early July 2020,causing mild to severe COVID-19 in millions.Monitoring the levels of antibodies such as immunoglobin(Ig)G,M and A that are specific to SARS-CoV-2 and present in the blood provides not only an alternative method for diagnosing SARS-CoV-2 infection(including asymptomatic carriers),but also a simple way to monitor immune responses in convalescent patients or after vaccination.A high and persistent level of antibodies specific to SARS-CoV-2,especially those that can bind to and neutralize the virus,would be a strong indication that an immunized host could resist to SRAS-CoV-2 infection.Currently,there are no effective drugs to specifically prevent or cure SARS-CoV-2 infection;therefore,host immune responses and antibody-based therapeutics will continue to play important roles in combating and later preventing COVID-19. | Huan Ma Dan Zhao Weihong Zeng Yunru Yang Xiaowen Hu Peigen Zhou Jianping Weng Linzhao Cheng Xueying Zheng Tengchuan Jin | 2021 | Science China(Life Sciences)2021，64，3： | 0 |
| 16 | Multi-Objective Prediction and Optimization of Vehicle Acoustic Package Based on ResNet Neural Network显示文摘Vehicle interior noise has emerged as a crucial assessment criterion for automotive NVH(Noise,Vibration,and Harshness).When analyzing the NVH performance of the vehicle body,the traditional SEA(Statistical Energy Analysis)simulation technology is usually limited by the accuracy of the material parameters obtained during the acoustic package modeling and the limitations of the application conditions.In order to effectively solve these shortcomings,based on the analysis of the vehicle noise transmission path,a multi-level objective decomposition architecture of the interior noise at the driver’s right ear is established.Combined with the data-driven method,the ResNet neural network model is introduced.The stacked residual blocks avoid the problem of gradient dis-appearance caused by the increasing network level of the traditional CNN network,thus establishing a higher-precision prediction model.This method alleviates the inherent limitations of traditional SEA simulation design,and enhances the prediction performance of the ResNet model by dynamically adjusting the learning rate.Finally,the proposed method is applied to a specific vehicle model and verified.The results show that the proposed meth-od has significant advantages in prediction accuracy and robustness. | Yunru Wu Xiangbo Liu Haibo Huang Yudong Wu Weiping Ding Mingliang Yang | 2023 | Sound & Vibration2023，57，1： | 0 |
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