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| 1 | Mosquito-Associated Viruses in China显示文摘Mosquitoes are classified into approximately 3500 species and further grouped into 41 genera.Epidemiologically,they are considered to be among the most important disease vectors in the world and they can harbor a wide variety of viruses.Several mosquito viruses are considered to be of significant medical importance and can cause serious public health issues throughout the world.Such viruses are Japanese encephalitis virus(JEV),dengue virus(DENV),chikungunya virus(CHIKV),and Zika virus(ZIKV).Others are the newly recognized mosquito viruses such as Banna virus(BAV)and Yunnan orbivirus(YNOV)with unclear medical significance.The remaining mosquito viruses are those that naturally infect mosquitoes but do not appear to infect humans or other vertebrates.With the continuous development and improvement of mosquito and mosquito-associated virus surveillance systems in China,many novel mosquito-associated viruses have been discovered in recent years.This review aims to systematically outline the history,characteristics,distribution,and/or current epidemic status of mosquito-associated viruses in China. | Han Xia Yujuan Wang Evans Atoni Bo Zhang Zhiming Yuan | 2018 | Virologica Sinica2018，33，1： | 13 |
| 2 | Great progress in study on aerosol and its impact on the global environment显示文摘The transport from atmosphere is a more important pathway than that from rivers for certain elements and compounds in the remote ocean. The desert and those arid/semi-arid areas in the northwest of China and the Loess Plateau are the sources of those aerosols over the Pacific. Atmospheric iron is the limiting factor of the productivity in certain ocean regions. The dust storms must now be seen as repeated sources of pollution elements as well as soil elements to the East China Sea and, farther out, to the North Pacific Ocean and even to the USA. The long-range transport of aerosols, especially the dust storm, from China and the positive feedbacks of iron coupled with sulfur in atmosphere might be one of the important mechanisms that would affect the primary productivity in the Pacific and/or the global climate change and deserve to be further studied. It can be seen clearly that the dust storms would affect not only the health of human beings and the local or regional climate, but also the global climate change that has been the focus of environmental study internationally. | ZHUANG Guoshun, HUANG Ronghui, WANG Mingxing, Q. ZHOU, GUO Jinghua, YUAN Hui and RAO Zhiming(1. The Center for Atmospheric Environmental Study, Beijing Normal University, Beijing 100875, China 2. The Institute of Atmospheric Physics, Chinese Academy of Sciences, Beijing 100029, China 3. University of New Mexico, Albuquerque, NM 87131, USA 4. Zhangzhou Teachers College, Zhangzhou 363000, China) | 2002 | Progress in Natural Science:Materials International2002，12，6： | 12 |
| 3 | Rapid Detection of Filoviruses by Real-time TaqMan Polymerase Chain Reaction Assays显示文摘Ebola virus (EBOV) and Marburg virus (MARV) are causative agents of severe hemorrhagic fever with high mortality rates in humans and non-human primates and there is currently no licensed vaccine or therapeutics. To date, there is no specific laboratory diagnostic test in China, while there is a national need to provide differential diagnosis during outbreaks and for instituting acceptable quarantine procedures. In this study, the TaqMan RT-PCR assays targeting the nucleoprotein genes of the Zaire Ebolavirus (ZEBOV) and MARV were developed and their sensitivities and specificities were investigated. Our results indicated that the assays were able to make reliable diagnosis over a wide range of virus copies from 103 to 109, corresponding to the threshold of a standard RNA transcript. The results showed that there were about 1010 RNA copies per milliliter of virus culture supernatant, equivalent to 10,000 RNA molecules per infectious virion, suggesting the presence of many non-infectious particles. These data indicated that the TaqMan RT-PCR assays developed in this study will be suitable for future surveillance and specific diagnosis of ZEBOV and MARV in China. | Yi Huang Hongping Wei Yunpeng Wang Zhengli Shi Herve Raoul Zhiming Yuan | 2012 | Virologica Sinica2012，27，5： | 10 |
| 4 | Analysis of threshed rice mixture separation through vibration screen using discrete element method显示文摘The discrete element method was utilized to construct three-dimensional discrete element models for the rice mixture,and their motions were analyzed numerically on a planar vibration screening device.The results showed that,after falling onto the vibrating screen surface,the mixture undergoes a reciprocating motion within the same cycle.During the screening process,the mixture undergoes segregation,slides along the screen surface,passes through the screen and then falls.In comparing the movement of grains and shriveled grains,it can be seen that the velocity of shriveled grains experiences cyclical changes,which is consistent with the grains’motion cycle.The impact on grains is shown to be greater than that on shriveled grains,and the average speeds of shriveled grains and grains converge.The curve shows that the screening of repetitive movements has a significant effect on the average velocity of shriveled grains,but also the velocity of shriveled grains and the vibration parameters can be well represented by a fitting equation.It is beneficial for the separation of grains from shriveled grains to choose a greater vibration frequency and screen surface inclination in the range of commonly used. | Li Hua Wang Jinshuang Yuan Jianbo Yin Wenqing Wang Zhiming Qian Youzhang | 2017 | International Journal of Agricultural and Biological Engineering2017，10，6： | 10 |
| 5 | Hierarchical assembly of highly efficient visible-light-driven Ag/g-C_(3)N_(4)/kaolinite composite photocatalyst for the degradation of ibuprofen显示文摘A novel Ag/g-C_(3)N_(4)/kaolinite composite photocatalyst was fabricated for the first time through a two-step assembly strategy by employing in situ calcination and a photodeposition process.The synthesized Ag/g-C_(3)N_(4)/kaolinite composite reached a higher degradation rate of ibuprofen(IBP)with a reaction rate constant of 0.0113 min^(-1) at an Ag content of 7% under visible-light irradiation,which was approximately 1.87 times that of the Ag/g-C_(3)N_(4) composite.Based on the physicochemical properties,the enhanced photocatalytic activity was attributed to the stronger adsorption property,wider photoresponse range and more efficient separation and transfer of electron-hole pairs.Furthermore,the incorporation of monodispersed Ag nanoparticles onto the g-C_(3)N_(4)/kaolinite sheets provided more reactive sites for the IBP degradation.In addition,according to the EPR study and trapping experiments,it was demonstrated that holes(h^(+))should be the key reactive species.A possible pathway of IBP degradation was also proposed based on the detected intermediates.Overall,the results of this work may facilitate the design of a novel visible-light-driven photocatalyst with a high efficiency that is derived from a natural mineral for environmental remediation. | Zhiming Sun Xiangwei Zhang Xiongbo Dong Xiaorui Liu Ye Tan Fang Yuan Shuilin Zheng Chunquan Li | 2020 | Journal of Materiomics2020，6，3： | 8 |
| 6 | Micro-droplet Digital Polymerase Chain Reaction and Real-Time Quantitative Polymerase Chain Reaction Technologies Provide Highly Sensitive and Accurate Detection of Zika Virus显示文摘The establishment of highly sensitive diagnostic methods is critical in the early diagnosis and control of Zika virus(ZIKV)and in preventing serious neurological complications of ZIKV infection. In this study, we established micro-droplet digital polymerase chain reaction(ddPCR) and real-time quantitative PCR(RT-qPCR) protocols for the detection of ZIKV based on the amplification of the NS5 gene. For the ZIKV standard plasmid, the RT-qPCR results showed that the cycle threshold(Ct) value was linear from 10~1 to 10~8 copy/l L, with a standard curve R^2 of 0.999 and amplification efficiency of 92.203%;however, a concentration as low as 1 copy/l L could not be detected. In comparison with RT-qPCR, the dd PCR method resulted in a linear range of 10~1–10~4 copy/l L and was able to detect concentrations as low as 1 copy/l L. Thus, for detecting ZIKV from clinical samples, RT-qPCR is a better choice for high-concentration samples(above 10~1 copy/l L),while ddPCR has excellent accuracy and sensitivity for low-concentration samples. These results indicate that the ddPCR method should be of considerable use in the early diagnosis, laboratory study, and monitoring of ZIKV. | Yuan Hui Zhiming Wu Zhiran Qin Li Zhu Junhe Liang Xujuan Li Hanmin Fu Shiyu Feng Jianhai Yu Xiaoen He Weizhi Lu Weiwei Xiao Qinghua Wu Bao Zhang Wei Zhao | 2018 | Virologica Sinica2018，33，3： | 6 |
| 7 | Identification of odorous compounds in reclaimed water using FPA combined with sensory GC-MS显示文摘Odorous compounds in the influent of a reclaimed water treatment plant (RWTP),consisting of coagulation,sedimentation,continuous micro-filtration (CMF),and chlorination in succession,in a north China city,were identified by combining flavor profile analysis (FPA) with sensory gas chromatograph-mass spectrometry (GC-MS).The sewery/swampy/septic odor with an odor intensity of 6.4 was found to be the major odor group in the RWTP influent,and the existence of well-known odorant including dimethyl disulfide,dimethyl trisulfide,indole and skatole were confirmed using GC-MS.The result of a spiking test showed that the intensity (3.6) of the sewery/swampy/septic odor caused by these four chemicals contributed to over 50% of the odor intensity of the influent.The FPA intensity for sewery/swampy/septic odor in the RWTP effluent was 3.8,showing that the treatment process was not efficient for the removal of odorants,particularly indole and skatole. | Zhiming Yan Yu Zhang Jianwei Yu Hongying Yuan Min Yang | 2011 | Journal of Environmental Sciences2011，23，10： | 6 |
| 8 | Nucleoprotein-based indirect enzyme-linked immunosorbent assay(indirect ELISA) for detecting antibodies specific to Ebola virus and Marbug virus显示文摘Full-length nucleoproteins from Ebola and Marburg viruses were expressed as His-tagged recombinant proteins in Escherichia coli and nucleoprotein-based enzyme-linked immunosorbent assays(ELISAs) were established for the detection of antibodies specific to Ebola and Marburg viruses. The ELISAs were evaluated by testing antisera collected from rabbit immunized with Ebola and Marburg virus nucleoproteins. Although little cross-reactivity of antibodies was observed in antiEbola virus nucleoprotein rabbit antisera, the highest reactions to immunoglobulin G(Ig G) were uniformly detected against the nucleoprotein antigens of homologous viruses. We further evaluated the ELISA's ability to detect antibodies to Ebola and Marburg viruses using human sera samples collected from individuals passing through the Guangdong port of entry. With a threshold set at the mean plus three standard deviations of average optical densities of sera tested, the ELISA systems using these two recombinant nucleoproteins have good sensitivity and specificity. These results demonstrate the usefulness of ELISA for diagnostics as well as ecological and serosurvey studies of Ebola and Marburg virus infection. | Yi Huang Youjie Zhu Mengshi Yang Zhenqing Zhang Donglin Song Zhiming Yuan | 2014 | Virologica Sinica2014，29，6： | 4 |
| 9 | Current status and future challenges of high-level biosafety laboratories in China显示文摘High-level biosafety laboratories are safe and secure platforms which integrate reliable containment,well-trained personnel,and specific biosafety manuals and practices to protect researchers from being infected while manipulating microbial pathogens and prevent pathogens from being released into the outside environment.During the past decades,laboratories with different protection levels have been constructed and operated,the legal framework and a laboratory biosafety management system have been established,and these operational laboratories have played an essential role in combatting emerging and re-emerging infectious diseases in China.Here,we have summarized the significant achievements of high-level laboratory planning,construction,and operation in China,as well as the challenges that we face in the future.Additionally,we have briefly discussed the national plan for construction of highlevel biosafety laboratories from 2016-2025 and‘‘biosafety-motivated”management system.This review might be informative for further understanding the present situation and future function of high-level biosafety laboratories in China. | Yuan Zhiming | 2019 | Journal of Biosafety and Biosecurity2019，1，2： | 4 |
| 10 | Pluripotent Stem Cell-derived Strategies to Treat Acute Liver Failure: Current Status and Future Directions显示文摘Liver disease has long been a heavy health and economic burden worldwide.Once the disease is out of control and progresses to end-stage or acute organ failure,orthotopic liver transplantation(OLT)is the only therapeutic alternative,and it requires appropriate donors and aggressive administration of immunosuppressive drugs.Therefore,hepatocyte transplantation(HT)and bioartificial livers(BALs)have been proposed as effective treatments for acute liver failure(ALF)in clinics.Although human primary hepatocytes(PHs)are an ideal cell source to support these methods,the large demand and superior viability of PH is needed,which restrains its wide usage.Thus,a finding alternative to meet the quantity and quality of hepatocytes is urgent.In this context,human pluripotent stem cells(PSC),which have unlimited proliferative and differential potential,derived hepatocytes are a promising renewable cell source.Recent studies of the differentiation of PSC into hepatocytes has provided evidence that supports their clinical application.In this review,we discuss the recent status and future directions of the potential use of PSC-derived hepatocytes in treating ALF.We also discuss opportunities and challenges of how to promote such strategies in the common applications in clinical treatments. | Jingfeng Liu Zhiming Yuan Qingwen Wang | 2022 | Journal of Clinical and Translational Hepatology2022，10，4： | 4 |
| 11 | Critical role of Dengue Virus NS1 protein in viral replication显示文摘Dengue virus(DENV) nonstructural protein 1(NS1) is a highly conserved 46-kDa protein that contains 2 glycosylation sites(Asn-130 and Asn-207) and 12 conserved cysteine(Cys) residues. Here, we performed site-directed mutagenesis to generate systematic mutants of viral strain TSV01. The results of the subsequent analysis showed that an alanine substitution at the second N-linked glycan Asn-207 in NS1 delayed viral RNA synthesis, reduced virus plaque size, and weakened the cytopathic effect. Three mutants at Cys sites(Cys-4, Cys-55, Cys-291) and a C-terminal deletion(ΔC) mutant significantly impaired RNA synthesis, and consequently abolished viral growth, whereas alanine mutations at Asn-130 and Glu-173 resulted in phenotypes that were similar to the wild-type(WT) virus. Further analysis showed that the Asn-207 mutation slightly delayed viral replication. These results suggest that the three conserved disulfide bonds and the second N-linked glycan in NS1 are required for DENV-2 replication. | Jingjing Fan Yi Liu Zhiming Yuan | 2014 | Virologica Sinica2014，29，3： | 4 |
| 12 | History of and suggestions for China’s biosafety legislation显示文摘Biosafety has attracted an unprecedented amount of attention from the global community as an important element in national security.All biosafety issues are related to laws and therefore must be regulated accordingly.A preliminary biosafety legislation system based on and suitable for China’s actual conditions has been established.The provisions of numerous laws have provided legislative tools to manage biosafety issues.The State Council and relevant ministries have also exercised their respective functions and drafted codes and regulations concerning biosafety protection.These laws and regulations play important roles,but our nation still lacks a law solely dedicated to biosafety regulation,and the supervisory system is incomplete.To remedy this situation,relevant biotechnologies must be developed,issues related to biosafety must be thoroughly examined,and the best practices of other nations should be introduced,all of which will improve biosafety legislation in China. | Liang Huigang Xiang Xiaowei Ma Haixia Yuan Zhiming | 2019 | Journal of Biosafety and Biosecurity2019，1，2： | 3 |
| 13 | Highly effective H_(2)/D_(2)separation in a stable Cu-based metalorganic framework显示文摘A three-dimensional copper metal-organic framework with the rare chabazite(CHA)topology namely FJI-Y11 has been constructed with flexibly carboxylic ligand 5,5'-[(1,4-phenylenebis(methylene))bis(oxy)]diisophthalic acid(H_(4)L).FJI-Y11 exhibits high water stability with the pH range from 2 to 12 at temperature as high as 373 K.Importantly,FJI-Y11 also shows high efficiency of hydrogen isotope separation using dynamic column breakthrough experiments under atmospheric pressure at 77 K.Attributed to its excellent structural stability,FJI-Y11 possesses good regenerated performance and maintains high separation efficiency after three cycles of breakthrough experiments. | Yanan Si Xiang He Jie Jiang Zhiming Duan Wenjing Wang Daqiang Yuan | 2021 | Nano Research2021，14，2： | 3 |
| 14 | The influence of perforated plates on wave transmission and hydrodynamic performance of pontoon floating breakwater显示文摘In this study, a perforated pontoon floating breakwater(FB) consisting of an impermeable plate and a perforated plate was designed to untangle the effect of a perforated plate on wave transmission and hydrodynamic performance of floating breakwater. A series of 2-D physical model experiments were conducted to measure the wave transmission coefficient, tension acting on the mooring line, and motion response of FB under a regular wave. The experimental results of the motion responses and mooring lines indicated that the new perforated plate was evidently effective. Furthermore, the study also discussed and analyzed the influence of the perforated plate on transmission coefficients. The experimental results showed that the new perforated plate did not lead to obvious improvement in the transmission | 嵇春艳 陈翔 马小剑 Zhiming Yuan | 2018 | Journal of Hydrodynamics2018，30，3： | 3 |
| 15 | Inhibition of Japanese Encephalitis Virus Infection by Flavivirus Recombinant E Protein Domain Ⅲ显示文摘Japanese encephalitis virus (JEV) is a mosquito-borne flavivirus closely related to the human pathogens including yellow fever virus, dengue virus and West Nile virus. There are currently no effective antiviral therapies for all of the flavivirus and only a few highly effective vaccines are licensed for human use. In this paper, the E protein domain III (DIII) of six heterologous flaviviruses (DENV1-4, WNV and JEV) was expressed in Escherichia coli successfully. The proteins were purified after a solubilization and refolding procedure, characterized by SDS-PAGE and Western blotting. Competitive inhibition showed that all recombinant flavivirus DIII proteins blocked the entry of JEV into BHK-21 cells. Further studies indicated that antibodies induced by the soluble recombinant flavivirus DIII partially protected mice against lethal JEV challenge. These results demonstrated that recombinant flavivirus DIII proteins could inhibit JEV infection competitively, and immunization with proper folding flavivirus DIII induced cross-protection against JEV infection in mice, implying a possible role of DIII for the cross-protection among flavivirus as well as its use in antigens for immunization in animal models. | Jingjing Fan Yi Liu Xuping Xie Bo Zhang Zhiming Yuan | 2013 | Virologica Sinica2013，28，3： | 3 |
| 16 | Occurrence of psychrotolerant Bacillus cereus group strains in ice creams显示文摘 | Guoping Zhou Dasheng Zheng Lina Dou Quanxin Cai Zhiming Yuan | 2009 | International Journal of Food Microbiology2009，，2： | 2 |
| 17 | Large-scale multiferroic complex oxide epitaxy with magnetically switched polarization enabled by solution processing显示文摘Complex oxides with tunable structures have many fascinating properties,though high-quality complex oxide epitaxy with precisely controlled composition is still out of reach.Here we have successfully developed solution-based single-crystalline epitaxy for multiferroic(1-x)BiTi(1-y)/2FeyMg(1-y)/2O3–(x)CaTiO3(BTFM–CTO)solid solution in large area,confirming its ferroelectricity at the atomic scale with strong spontaneous polarization.Careful compositional tuning leads to a bulk magnetization of 0.07±0.035μB/Fe at room temperature,enabling magnetically induced polarization switching exhibiting a large magnetoelectric coefficient of 2.7–3.0×10^-7 s/m.This work demonstrates the great potential of solution processing in large-scale complex oxide epitaxy and establishes novel room-temperature magnetoelectric coupling in epitaxial BTFM–CTO film,making it possible to explore a much wider space of composition,phase,and structure that can be easily scaled up for industrial applications. | Cong Liu Feng An Paria S.M.Gharavi Qinwen Lu Junkun Zha Chao Chen Liming Wang Xiaozhi Zhan Zedong Xu Yuan Zhang Ke Qu Junxiang Yao Yun Ou Zhiming Zhao Xiangli Zhong Dongwen Zhang Nagarajan Valanoor Lang Chen Tao Zhu Deyang Chen Xiaofang Zhai Peng Gao Tingting Jia Shuhong Xie Gaokuo Zhong Jiangyu Li | 2020 | National Science Review2020，7，1： | 2 |
| 18 | Enhanced visible-light-assisted peroxymonosulfate activation over MnFe_(2)O_(4) modified g-C_(3)N_(4)/diatomite composite for bisphenol A degradation显示文摘The MnFe_(2) O_(4)/g-C_(3) N_(4)/diatomite composites(Mn/G/D) were prepared via a facile precipitation-calcination method in this study.The Mn/G/D possessed higher specific surface area,lower electron-hole pairs' recombination rate,as well as wider and stronger visible light absorption capacity.Since the synergistic effect between g-C_(3 )N_(4) and MnFe_(2) O_(4),the photogene rated electron could transfer from g-C3 N4 to MnFe_(2) O_(4),which could promote the migration of electrons as well as enhance the photocatalytic activity and peroxymonosulfate(PMS) activation efficiency.Mn/G/D-5% composite displayed the excellent degradation performance of bisphenol A(BPA) with the removal efficiency of 99.9% under PMS/Vis system,which was approximately 2.47 and 63.8 times as high as that of the Mn/G/D-5%/PMS and Mn/G/D-5%/Vis system,respectively.Moreover,negative electricity derived from diatomite surface also promoted the photogenerated carriers' migration,and the degradation rate constant was around 2.4 times higher than that of MnFe_(2) O_(4)/g-C_(3) N_(4)(Mn/G).In addition,quenching experiments showed that both radical pathway(h^(+),·OH,·O_(2)^(-)and SO_(4)·^(-)) and non-radical pathway(^(1) O_(2)) were responsible for the degradation of BPA. | Xiangwei Zhang Chunquan Li Ting Chen Ye Tan Xiaorui Liu Fang Yuan Shuilin Zheng Zhiming Sun | 2021 | International Journal of Mining Science and Technology2021，31，6： | 2 |
| 19 | Impact assessment of modified clay on embryo-larval stages of turbot Scophthalmus maximus L.显示文摘Using modified clay is one of the most promising methods for the mitigation of harmful algal blooms(HABs).However,the environmental impact of modified clay has become a subject of global concern.In this study,turbot(Scophthalmus maximus L.)embryos were used as a model to assess the effect of modified clay on this sensitive stage of fish development.Results show that the 24 and 48h LC 50(median lethal concentrations)of the modified clay were 1.70 and 1.65 g/L,and the safe concentration was 0.47 g/L,which is much higher than the concentration used to treat HAB.The modified clay did not affect significantly the hatchability of turbot embryos but when the concentration exceeded 0.50 g/L,the deformity rate of newly hatched larvae increased significantly.The total length,specific growth rate(SGR)and yolk sac absorption rate of larvae reached their peaks at 0.50 g/L and then gradually decreased as the concentration of modified clay increased.Therefore,a moderate amount of modified clay does not harm the survival and hatching of turbot embryos,or the growth and development of newly hatched larvae. | ZHANG Yue SONG Xiuxian YU Zhiming ZHANG Peipei CAO Xihua YUAN Yongquan | 2019 | Journal of Oceanology and Limnology2019，37，3： | 2 |
| 20 | Mutagenesis of D80-82 and G83 Residues in West Nile Virus NS2B: Effects on NS2B-NS3 Activity and Viral Replication显示文摘Flaviviral NS2B is a required cofactor for NS3 serine protease activity and plays an important role in promoting functional NS2B-NS3 protease configuration and maintaining critical interactions with protease catalysis substrates. The residues D80DDG in West Nile virus (WNV) NS2B are important for protease activity. To investigate the effects of D80DDG in NS2B on protease activity and viral replication, the negatively charged region D80DD and the conserved residue G83 of NS2B were mutated (D80DD/E80EE, D80DD/K80KK, D80DD/A80AA, G83F, G83S, G83D, G83K, and G83A), and NS3 D75A was designated as the negative control. The effects of the mutations on NS2B-NS3 activity, viral translation, and viral RNA replication were analyzed using kinetic analysis of site-directed enzymes and a transient replicon assay. All substitutions resulted in significantly decreased enzyme activity and blocked RNA replication. The negative charge of D80DD is not important for maintaining NS2B function, but side chain changes in G83 have dramatic effects on protease activity and RNA replication. These results demonstrate that NS2B is important for viral replication and that D80DD and G83 substitutions prevent replication; they will be useful for understanding the relationship between NS2B and NS3. | Fan Jia Jingjing Fan Bo Zhang Zhiming Yuan | 2013 | Virologica Sinica2013，28，1： | 1 |
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