|
|
|
题名
|
作者
|
年代
|
出处
|
被引量
|
| 1 | SlMYB75,an MYB-type transcription factor,promotes anthocyanin accumulation and enhances volatile aroma production in tomato fruits显示文摘Genetic manipulation of genes to upregulate specific branches of metabolic pathways is a method that is commonly used to improve fruit quality.However,the use of a single gene to impact several metabolic pathways is difficult.Here,we show that overexpression of the single gene SlMYB75(SlMYB75-OE)is effective at improving multiple fruit quality traits.In these engineered fruits,the anthocyanin content reached 1.86mg g−1 fresh weight at the red-ripe stage,and these SlMYB75-OE tomatoes displayed a series of physiological changes,including delayed ripening and increased ethylene production.In addition to anthocyanin,the total contents of phenolics,flavonoids and soluble solids in SlMYB75-OE fruits were enhanced by 2.6,4,and 1.2 times,respectively,compared to those of wild-type(WT)fruits.Interestingly,a number of aroma volatiles,such as aldehyde,phenylpropanoid-derived and terpene volatiles,were significantly increased in SlMYB75-OE fruits,with some terpene volatiles showing more than 10 times higher levels than those in WT fruits.Consistent with the metabolic assessment,transcriptomic profiling indicated that the genes involved in the ethylene signaling,phenylpropanoid and isoprenoid pathways were greatly upregulated in SlMYB75-OE fruits.Yeast one-hybrid and transactivation assays revealed that SlMYB75 is able to directly bind to the MYBPLANT and MYBPZM cis-regulatory elements and to activate the promoters of the LOXC,AADC2 and TPS genes.The identification of SlMYB75 as a key regulator of fruit quality attributes through the transcriptional regulation of downstream genes involved in several metabolic pathways opens new avenues towards engineering fruits with a higher sensory and nutritional quality. | Wei Jian Haohao Cao Shu Yuan Yudong Liu Juanfang Lu Wang Lu Ning Li Jianhui Wang Jian Zou Ning Tang Chan Xu Yulin Cheng Yanqiang Gao Wanpeng Xi Mondher Bouzayen Zhengguo Li | 2019 | Horticulture Research2019，6，1： | 39 |
| 2 | Study of Bacillus subtilis on growth performance, nutrition metabolism and intestinal microflora of 1 to 42 d broiler chickens显示文摘To study the influence of different levels of Bacillus subtilis on growth performance, nutrition metabolism and intestinal microflora of 1 to 42 d Arbor Acres(AA) broilers, a total of 800 one-day-old healthy broilers were randomly divided into 5 groups with 4 replicates per group and 40 broilers per replicate. Broilers were fed a basic diet(group 1) which acted as the control group, and 4 other groups(2 to 5) were fed the basal diet with B. subtilis added at concentrations of 100, 150, 200 and 250 mg/kg, respectively for 42 days. The results showed as follow: the average daily gain(ADG) of group 4 was significantly higher than(P < 0.05) that of group 1, and the average daily feed intake(ADFI) of group 5 was the highest but the differences between groups were not significant(P > 0.05). The feed to gain ratio(F/G) of all the experimental groups was lower than that of the control and the difference was significant in group 4(P < 0.05). In addition, supplementation of B. subtilis increased the apparent metabolism of crude protein(P > 0.05), crude fat(P > 0.05), dry matter(P > 0.05) and organic matter(P < 0.05). B. subtilis decreased the Escherichia coli and Salmonella populations in the cecum. This shows that adding B. subtilis to the broiler diet can improve the growth performance, increase feed efficiency, regulate serum index and reduce harmful bacteria in the intestinal tract. Based on our study, it could be recommended that addition of B. subtilis at 200 mg/kg could improve the growth performance of broilers. | Zhenhua Gao Haohao Wu Lin Shi Xiaohui Zhang Ran Sheng Fuquan Yin Ravi Gooneratne | 2017 | Animal Nutrition2017，，2： | 23 |
| 3 | rMVP: A Memory-efficient, Visualization-enhanced, and Parallel-accelerated Tool for Genome-wide Association Study显示文摘Along with the develoipment of high-throughput sequencing technologies,both sample size and SNP number are increasing rapidly in genome-wide association studies(GWAS),and the associated computation is more challenging than ever.Here,we present a memory-efficient,visualization-enhanced,and parallel-accelerated R package called“r MVP”to address the need for improved GWAS computation.r MVP can 1)effectively process large GWAS data,2)rapidly evaluate population structure,3)efficiently estimate variance components by Efficient Mixed-Model Association e Xpedited(EMMAX),Factored Spectrally Transformed Linear Mixed Models(Fa ST-LMM),and Haseman-Elston(HE)regression algorithms,4)implement parallel-accelerated association tests of markers using general linear model(GLM),mixed linear model(MLM),and fixed and random model circulating probability unification(Farm CPU)methods,5)compute fast with a globally efficient design in the GWAS processes,and 6)generate various visualizations of GWASrelated information.Accelerated by block matrix multiplication strategy and multiple threads,the association test methods embedded in r MVP are significantly faster than PLINK,GEMMA,and Farm CPU_pkg.r MVP is freely available at https://github.com/xiaolei-lab/r MVP. | Lilin Yin Haohao Zhang Zhenshuang Tang Jingya Xu Dong Yin Zhiwu Zhang Xiaohui Yuan Mengjin Zhu Shuhong Zhao Xinyun Li Xiaolei Liu | 2021 | Genomics, Proteomics & Bioinformatics2021，19，4： | 12 |
| 4 | Evaluation of spinal cord injury animal models显示文摘Because there is no curative treatment for spinal cord injury,establishing an ideal animal model is important to identify injury mechanisms and develop therapies for individuals suffering from spinal cord injuries.In this article,we systematically review and analyze various kinds of animal models of spinal cord injury and assess their advantages and disadvantages for further studies. | Ning Zhang Marong Fang Haohao Chen Fangming Gou Mingxing Ding | 2014 | Neural Regeneration Research2014，9，22： | 10 |
| 5 | Energy-efficient power allocation for non-regenerative OFDM relay links显示文摘We consider a two-hop non-regenerative (amplify-and-forward) relay link with one source, one destination and one relay, in which orthogonal frequency division multiplexing (OFDM) is used. The power allocation (PA) to maximize the energy efficiency for the whole link is investigated under quality of service (Qos) require- ment and separate power constraints. An approximation method with low complexity is first proposed to convert the PA problem into a quasi-concave problem, which can obtain a tight lower bound in high SNR region. Furthermore, a more accurate approximation method with higher complexity is raised, which can approach the actual performance both in the low SNR and high SNR regions. We also introduce subcarrier pairing scheme to further enhance the energy efficiency for the link. Simulation results are shown to compare the performance of the two proposed methods. | YU Huan QIN HaoHao LI YunZhou ZHAO YiFei XU XiBin WANG Jing | 2013 | Science China(Information Sciences)2013，56，2： | 8 |
| 6 | Influence of leading edge with real manufacturing error on aerodynamic performance of high subsonic compressor cascades显示文摘To investigate the influence of real leading-edge manufacturing error on aerodynamic performance of high subsonic compressor blades,a family of leading-edge manufacturing error data were obtained from measured compressor cascades.Considering the limited samples,the leadingedge angle and leading-edge radius distribution forms were evaluated by Shapiro-Wilk test and quantile–quantile plot.Their statistical characteristics provided can be introduced to later related researches.The parameterization design method B-spline and Bezier are adopted to create geometry models with manufacturing error based on leading-edge angle and leading-edge radius.The influence of real manufacturing error is quantified and analyzed by self-developed non-intrusive polynomial chaos and Sobol’indices.The mechanism of leading-edge manufacturing error on aerodynamic performance is discussed.The results show that the total pressure loss coefficient is sensitive to the leading-edge manufacturing error compared with the static pressure ratio,especially at high incidence.Specifically,manufacturing error of the leading edge will influence the local flow acceleration and subsequently cause fluctuation of the downstream flow.The aerodynamic performance is sensitive to the manufacturing error of leading-edge radius at the design and negative incidences,while it is sensitive to the manufacturing error of leading-edge angle under the operation conditions with high incidences. | Chi MA Limin GAO Haohao WANG Ruiyu LI Baohai WU | 2021 | Chinese Journal of Aeronautics2021，34，6： | 7 |
| 7 | Mg-Zn-Y-Nd coated with citric acid and dopamine by layer-by-layer self-assembly to improve surface biocompatibility显示文摘Magnesium alloy has been generally accepted as an important biodegradable material on cardiovascular stent development for a long time. However, its limited biocompatibility, especially delayed endothelialization process restricts its further application. In this contribution, we modified the Mg-Zn-Y-Nd alloy surface with citric acid and dopamine via a layer-by-layer self-assembly assay, aiming at improving the biocompatibility of the magnesium alloy. The citric acid/dopamine(CA/PDA) layer exhibited a remarkable suppression of platelet activation/aggregation and thrombosis under 15 dyn/cm2 blood flowing. Inhibition on vascular smooth muscle cells growth and macrophages attachment/activation were also observed on this layer. In particular, the CA/PDA layer presented a promoted property for the vascular endothelial cells growth and spreading compared with the bare magnesium alloy, suggesting the pro-endotelialized function. In conclusion, this research may support potential application on surface modification of magnesium alloy based cardiovascular stents for better biocompatibility. | CHEN Li LI JingAn CHANG JiaWei JIN ShiBo WU Di YAN HaoHao WANG XiaoFeng GUAN ShaoKang | 2018 | Science China(Technological Sciences)2018，61，8： | 7 |
| 8 | Advanced smart biomaterials and constructs for hard tissue engineering and regeneration显示文摘Hard tissue repair and regeneration cost hundreds of billions of dollars annually worldwide, and the need has substantially increased as the population has aged. Hard tissues include bone and tooth structures that contain calcium phosphate minerals.Smart biomaterial-based tissue engineering and regenerative medicine methods have the exciting potential to meet this urgent need. Smart biomaterials and constructs refer to biomaterials and constructs that possess instructive/inductive or triggering/stimulating effects on cells and tissues by engineering the material's responsiveness to internal or external stimuli or have intelligently tailored properties and functions that can promote tissue repair and regeneration. The smart material-based approaches include smart scaffolds and stem cell constructs for bone tissue engineering; smart drug delivery systems to enhance bone regeneration; smart dental resins that respond to pH to protect tooth structures; smart pH-sensitive dental materials to selectively inhibit acid-producing bacteria; smart polymers to modulate biofilm species away from a pathogenic composition and shift towards a healthy composition; and smart materials to suppress biofilms and avoid drug resistance. These smart biomaterials can not only deliver and guide stem cells to improve tissue regeneration and deliver drugs and bioactive agents with spatially and temporarily controlled releases but can also modulate/suppress biofilms and combat infections in wound sites. The new generation of smart biomaterials provides exciting potential and is a promising opportunity to substantially enhance hard tissue engineering and regenerative medicine efficacy. | Ke Zhang Suping Wang Chenchen Zhou Lei Cheng Xianling Gao Xianju Xie Jirun Sun Haohao Wang Michael D.Weir Mark A.Reynolds Ning Zhang Yuxing Bai Hockin H.K.Xu | 2018 | Bone Research2018，6，4： | 6 |
| 9 | Effect of vegetation type, wetting intensity, and nitrogen supply on external carbon stimulated heterotrophic respiration and microbial biomass carbon in forest soils显示文摘By using packed soil-core incubation experiments,we have studied stimulating effects of addition of external carbon(C)(glucose,6.4 g Cm^(-2))on heterotrophic respiration and microbial biomass C of a mature broadleaf and Korean pine mixed forest(BKPF)and an adjacent white birch forest(WBF)soil under different wetting intensities(55%and 80%WFPS,water-filled pore space)and nitrogen(N)supply(NH_4C1 and KNO_3,4.5 g Nm^(-2))conditions.The results showed that for the control,the cumulative carbon dioxide(CO_2)flux from WBF soil during the 15-day incubation ranged from 5.44 to 5.82 g CO_2-Cm^(-2),which was significantly larger than that from BKPF soil(2.86 to 3.36 g CO_2-Cm^(-2)).With increasing wetting intensity,the cumulative CO_2 flux from the control was decreased for the WBF soil,whereas an increase in the CO_2 flux was observed in the BKPF soil(P<0.05).The addition of NH_4C1 or KNO_3 alone significantly reduced the cumulative CO_2 fluxes by 9.2%-21.6%from the two soils,especially from WBF soil at low wetting intensity.The addition of glucose alone significantly increased soil heterotrophic respiration,microbial biomass C(MBC),and microbial metabolic quotient.The glucose-induced cumulative CO_2 fluxes and soil MBC during the incubation ranged from 8.7 to 11.7 g CO_2-Cm^(-2)and from 7.4 to 23.9 g Cm^(-2),which are larger than the dose of added C.Hence,the addition of external carbon can increase the decomposition of soil native organic C.The glucose-induced average and maximum rates of CO_2 fluxes during the incubation were significantly influenced by wetting intensity(WI)and vegetation type(VT),and by WI×VT,NH_4Cl×VT and WI×VT×NH_4Cl(P<0.05).The addition of NH_4C1,instead of KNO_3 significantly decreased the glucose-induced MBC of WBF soil(P<0.05),whereas adding NH_4C1 and KNO_3 both significantly increased the glucose-induced MBC of BKPF soil at high moisture(P<0.05).According to the differences in soil labile C pools,MBC and CO_2 fluxes in the presence and absence of glucose,it can be concluded that the stimulating effects of glucose on soil heterotrophic respiration and MBC under temperate forests were dependent on vegetation type,soil moisture,and amount and type of the N added. | WU HaoHao XU XingKai DUAN CunTao LI TuanSheng CHENG WeiGuo | 2015 | Science China Earth Sciences2015，58，8： | 6 |
| 10 | Decision Support System for Adaptive Restoration Control of Transmission System显示文摘The power system restoration control has a higher uncertainty level than the preventive control of cascading failures. In order to ensure the feasibility of the decision support system of restoration control, a decision support framework for adaptive restoration control of transmission system is proposed, which can support the coordinated restoration of multiple partitions, coordinated restoration of units and loads, and coordination of multi-partition decision-making process and actual restoration process. The proposed framework is divided into two layers, global coordination layer and partition optimization layer. The upper layer partitions the transmission system according to the power outage scenario, constantly and dynamically adjusts the partitions during the restoration process, and optimizes the time-space decision-making of inter-partition connectivity. For each partition, the lower layer pre-selects restoration targets according to the estimated restoration income, optimizes the corresponding restoration paths, and evaluates the restoration plans according to the expected net income per unit of power consumption. During the restoration process, if the restoration operation such as energizing the outage branch fails, the current restoration plan will be adaptively switched to the sub-optimal one or re-optimized if necessary. The framework includes two operation modes, i.e., the on-line operation mode and training simulation mode, and provides an information interaction interface for collaborative restoration with related distribution systems. The effectiveness and adaptability of the proposed framework is demonstrated by simulations using the modified IEEE 118-bus system. | Ze Li Yusheng Xue Haohao Wang Lili Hao | 2021 | Journal of Modern Power Systems and Clean Energy2021，9，4： | 6 |
| 11 | Elevating H3K27me3 level sensitizes colorectal cancer to oxaliplatin显示文摘Histone methylation is a context-dependent modification that regulates gene expression,and the trimethylation of histone H3 lysine 27(H3K27me3)usually induces gene silencing.Overcoming colorectal cancer(CRC)chemoresistance is currently a huge challenge,but the relationship between H3K27me3 modification and chemoresistance remains largely unclear.Here,we found that H3K27me3 levels positively correlated with the metastasis-free survival of CRC patients and a low H3K27me3 level predicted a poor outcome upon chemotherapeutic drug treatment.Oxaliplatin stimulation significantly induced the expression of H3K27 lysine demethylase 6A/6B(KDM6A/6B),thus decreasing the level of H3K27me3 in CRC cells.Elevation of H3K27me3 level through KDM6A/6B depletion or GSK-J4(a KDM6A/6B inhibitor)treatment significantly enhanced oxaliplatin-induced apoptosis.Conversely,when inhibiting the expression of H3K27me3 by EPZ-6438,an inhibitor of the histone methyltransferase EZH2,the proportion of apoptotic cells remarkably decreased.In addition,the combination of GSK-J4 and oxaliplatin significantly inhibited tumor growth in an oxaliplatin-resistant patient-derived xenograft model.Importantly,we revealed that oxaliplatin treatment dramatically induced NOTCH2 expression,which was caused by downregulation of H3K27me3 level on the NOTCH2 transcription initiation site.Thus,the activated NOTCH signaling promoted the expression of stemness-related genes,which resulted in oxaliplatin resistance.Furthermore,oxaliplatin-induced NOTCH signaling could be interrupted by GSK-J4 treatment.Collectively,our findings suggest that elevating H3K27me3 level can improve drug sensitivity in CRC patients. | Qi Wang Xi Chen Yuhang Jiang Sanhong Liu Hanshao Liu Xiaohua Sun Haohao Zhang Zhi Liu Yu Tao Cuifeng Li Yiming Hu Dandan Liu Deji Ye Yongzhong Liu Mingliang Wang Xiaoren Zhang | 2020 | Journal of Molecular Cell Biology2020，12，2： | 6 |
| 12 | Potassium superoxide oxygen generation rate and carbon dioxide absorption rate in coal mine refuge chambers显示文摘The effects of the molding pressure of a KO2 oxygen plate and the initial concentration of CO2 on the oxygen generation rate, the oxygen generation efficiency, and the carbon dioxide absorption rate were studied using a YES-300 hydraulic press to alter the pressure when forming the oxygen plate used in a coal mine refuge chamber. In addition, changes in the initial concentration of CO2 used in the closedbox model were made by adjusting the CO2 supply system, and a CD-7 multi-function parameter instrument was employed to monitor and record the changes of O2 and CO2concentration in the closed-box model. Results indicate that the oxygen generation rate of KO2 oxygen plates, the oxygen generation efficiency, and the carbon dioxide absorption rate decrease when there is an increase in the pressure used to mold the oxygen plates, but those values increase when the initial CO2 concentration increases. When the initial concentration of CO2 in the closed-box model is 3.5% and the forming pressure is 10 k N, the average oxygen generation rate of 15 g KO2 oxygen plate is 11.88 10 3L/min, the oxygen generation efficiency is 80.3%, and the average CO2 absorption rate is 11.0 10 3L/min. Compared with the condition where the initial CO2 concentration is 1.5%, the results show that average oxygen generation rate of oxygen plates increases by 88.9%, the oxygen generation efficiency increases by 88.9%, and the CO2 absorption rate increases by 100%. | Gao Na Jin Longzhe Hu Haohao Huang Xiao Zhou Li Fan Linyu | 2015 | International Journal of Mining Science and Technology2015，25，1： | 4 |
| 13 | Outcome of Patients with Colorectal Liver Metastasis: Analysis of 1,613 Consecutive Cases显示文摘 | Zhu Dexiang Ren Li Wei Ye Wu Haifu Zhong Yunshi Ye Qinghai Zhai Shenyong Xu Bo Liang Li Pan Xiangou Li Haohao Ye Lechi Liu Tianshu Fan Jia Qin Xinyu Xu Jianmin | 2012 | Annals of Surgical Oncology2012，，9： | 3 |
| 14 | 3D printing of tissue engineering scaffolds:a focus on vascular regeneration显示文摘Tissue engineering is an emerging means for resolving the problems of tissue repair and organ replacement in regenerative medicine.Insufficient supply of nutrients and oxygen to cells in large-scale tissues has led to the demand to prepare blood vessels.Scaffold-based tissue engineering approaches are effective methods to form new blood vessel tissues.The demand for blood vessels prompts systematic research on fabrication strategies of vascular scaffolds for tissue engineering.Recent advances in 3D printing have facilitated fabrication of vascular scaffolds,contributing to broad prospects for tissue vascularization.This review presents state of the art on modeling methods,print materials and preparation processes for fabrication of vascular scaffolds,and discusses the advantages and application fields of each method.Specially,significance and importance of scaffold-based tissue engineering for vascular regeneration are emphasized.Print materials and preparation processes are discussed in detail.And a focus is placed on preparation processes based on 3D printing technologies and traditional manufacturing technologies including casting,electrospinning,and Lego-like construction.And related studies are exemplified.Transformation of vascular scaffolds to clinical application is discussed.Also,four trends of 3D printing of tissue engineering vascular scaffolds are presented,including machine learning,near-infrared photopolymerization,4D printing,and combination of self-assembly and 3D printing-based methods. | Pengju Wang Yazhou Sun Xiaoquan Shi Huixing Shen Haohao Ning Haitao Liu | 2021 | Bio-Design and Manufacturing2021，4，2： | 3 |
| 15 | Polarization microwave-induced thermoacoustic imaging for quantitative characterization of deep biological tissue microstructures显示文摘Polarization optical imaging can be used to characterize anisotropy in biological tissue microstructures and has been demonstrated to be a powerful tool for clinical diagnosis. However, the approach is limited by an inability to image targets deeper than ~1 mm due to strong optical scattering in biological tissues. As such, we propose a novel polarization microwave-induced thermoacoustic imaging(P-MTAI) technique to noninvasively detect variations in deep tissue by exploiting the thermoacoustic signals induced by four pulsed microwaves of varying polarization orientations. The proposed P-MTAI method overcomes the penetration limits of conventional polarization optical imaging and provides submillimeter resolution over depths of several centimeters. As part of the paper, the structural characteristics of tissues were quantified using a new parameter, the degree of microwave absorption anisotropy. P-MTAI was also applied to the noninvasive detection of morphological changes in cardiomyocytes as they transitioned from ordered to disordered states, providing a potential indication of myocardial infarction. | YUJING LI SHANXIANG ZHANG LINGHUA WU ZHONGWEN CHENG ZHENHUI ZHANG HAOHAO WANG SHUXIANG ZHAO MINGYANG REN SIHUA YANG DA XING HUAN QIN | 2022 | Photonics Research2022，10，5： | 2 |
| 16 | Natural variations of ZmSRO1d modulate the trade-off between drought resistance and yield by affecting ZmRBOHC-mediated stomatal ROS production in maize显示文摘While crop yields have historically increased,drought resistance has become a major concern in the context of global climate change.The trade-off between crop yield and drought resistance is a common phenomenon;however,the underlying molecular modulators remain undetermined.Through genome-wide association study,we revealed that three non-synonymous variants in a drought-resistant allele of ZmSRO1d-R resulted in plasma membrane localization and enhanced mono-ADP-ribosyltransferase activity of ZmSRO1d toward ZmRBOHC,which increased reactive oxygen species(ROS)levels in guard cells and promoted stomatal closure.ZmSRO1d-R enhanced plant drought resilience and protected grain yields under drought conditions,but it led to yield drag under favorable conditions.In contrast,loss-of-function mutants of ZmRBOHC showed remarkably increased yields under well-watered conditions,whereas they showed compromised drought resistance.Interestingly,by analyzing 189 teosinte accessions,we found that the ZmSRO1d-R allele was present in teosinte but was selected against during maize domestication and modern breeding.Collectively,our work suggests that the allele frequency reduction of ZmSRO1d-R in breeding programs may have compromised maize drought resistance while increased yields.Therefore,introduction of the ZmSRO1d-R allele into modern maize cultivars would contribute to food security under drought stress caused by global climate change. | Huajian Gao Junjun Cui Shengxue Liu Shuhui Wang Yongyan Lian Yunting Bai Tengfei Zhu Haohao Wu Yijie Wang Shiping Yang Xuefeng Li Junhong Zhuang Limei Chen Zhizhong Gong Feng Qin | 2022 | Molecular Plant2022，15，10： | 2 |
| 17 | Conodont Biostratigraphy across the Permian-Triassic Boundary at the Xinmin Section,Guizhou,South China显示文摘A continuous sedimentation at the Xinmin Section, Anshun, Guihzou Province, with the Upper Permian Talung Formation dominated by bedded siliceous rocks, and the Lower Triassic Luolou Formation consisting mudstones and marls as well as siliceous mudstones at its basal part indicates that it represents a deeper-water basinal facies across the Permian-Triassic boundary. Based on a systematic conodont biostratigraphic work, nine conodont species belong to two genera have been identified in this study. It enable us to establish five conodont zones at this section, in ascending order, they are: Clarkina changxingensis Zone(beds 1-3–4-2), Clarkina yini Zone(beds 4-3–5-1-1), Clarkina meishanensis Zone(beds 5-1-2–5-2), Hindeodus changxingensis Zone(beds 5-3-1–5-3-2) and Hindeodus parvus Zone(beds 5-3-3–5-3-4), respectively. According to the first occurrence of Hindeodus parvus in bed 5-3-3, the Permian-Triassic boundary is placed at the base of bed 5-3-3. This conodont zonation of the Xinmin Section provides precise biostratigraphic framework for further investigations on the geological events across the Permian-Triassic boundary at this section. In addition, the new conodont data also reveals that several siliceous beds occurred at the basal Triassic. It provides an exception of Early Triassic Chert Gap. | Ning Zhang Haishui Jiang Wenli Zhong Haohao Huang Wenchen Xia | 2014 | Journal of Earth Science2014，25，5： | 2 |
| 18 | Enhanced mechanical and thermal properties of γ-allyloxymethyl 18-crown-6 and polyimide composites through hydrosilylation crosslinking显示文摘The complexation of y-allyloxymethyl 18-crown-6(AC6) induced inferior mechanical and thermal properties of polyimide(PI) in spite of lowered dielectric constant(k). To solve this puzzle, tetrakis-(di methylsiloxy)-si lane was employed to crosslink the complex of AC6 and PI(AC6-PI) through hydrosilylation reaction. The crosslinked AC6-PI(SiAC-PI) composites possessed excellent mechanical and thermal properties as well as low k. The tensile strength and fracture energy of SiAC-PI were increased by 87% and 716%, and the glass transition temperature and 5% weight loss temperature elevated 14.5 ℃ and 38.8 ℃, respectively, compared with those of AC6-PI. The structure of SiAC-PI was characterized by FTIR spectra, crosslinked density and XRD diffraction patterns. | Chuqi Shi Shumei Liu Yang Li Jianqing Zhao Haohao Huang | 2019 | Chinese Chemical Letters2019，30，3： | 2 |
| 19 | Histones released by NETosis enhance the infectivity of SARS-CoV-2 by bridging the spike protein subunit 2 and sialic acid on host cells显示文摘Neutrophil extracellular traps(NETs)can capture and kill viruses,such as influenza viruses,human immunodeficiency virus(HIV),and respiratory syncytial virus(RSV),thus contributing to host defense.Contrary to our expectation,we show here that the histones released by NETosis enhance the infectivity of SARS-CoV-2,as found by using live SARS-CoV-2 and two pseudovirus systems as well as a mouse model.The histone H3 or H4 selectively binds to subunit 2 of the spike(S)protein,as shown by a biochemical binding assay,surface plasmon resonance and binding energy calculation as well as the construction of a mutant S protein by replacing four acidic amino acids.Sialic acid on the host cell surface is the key molecule to which histones bridge subunit 2 of the S protein.Moreover,histones enhance cell-cell fusion.Finally,treatment with an inhibitor of NETosis,histone H3 or H4,or sialic acid notably affected the levels of sgRNA copies and the number of apoptotic cells in a mouse model.These findings suggest that SARS-CoV-2 could hijack histones from neutrophil NETosis to promote its host cell attachment and entry process and may be important in exploring pathogenesis and possible strategies to develop new effective therapies for COVID-19. | Weiqi Hong Jingyun Yang Jun Zou Zhenfei Bi Cai He Hong Lei Xuemei He Xue Li Aqu Alu Wenyan Ren Zeng Wang Xiaohua Jiang Kunhong Zhong Guowen Jia Yun Yang Wenhai Yu Qing Huang Mengli Yang Yanan Zhou Yuan Zhao Dexuan Kuang Junbin Wang Haixuan Wang Siyuan Chen Min Luo Ziqi Zhang Tiangi Lu Li Chen Haiying Que Zhiyao He Qiu Sun Wei Wang Guobo Shen Guangwen Lu Zhiwei Zhao Li Yang Jinliang Yang Zhenling Wang Jiong Li Xiangrong Song Lunzhi Dai Chong Chen Jia Geng Maling Gou Lu Chen Haohao Dong Yong Peng Canhua Huang Zhiyong Qian Wei Cheng Changfa Fan Yuquan Wei Zhaoming Su Aiping Tong Shuaiyao Lu Xiaozhong Peng Xiawei Wei | 2022 | Cellular & Molecular Immunology2022，19，5： | 2 |
| 20 | Optimization of a doxycycline hydroxypropyl-β-cyclodextrin inclusion complex based on computational modeling显示文摘To prepare a stable complex of doxycycline(Doxy)and hydroxypropy-β-cyclodextrin(HP-β-CD)for ophthalmic delivery,the optimum formulation and preparation conditions were investigated using response surface methodology(RSM),artificial neural network(ANN)and support vector machine(SVM)modeling.The molar ratios of HP-β-CD/Doxy and Mg^(2+)/Doxy,inclusion time and temperature were selected as independent variables (X_(1)-X_(4)) and inclusion efficiency and stability of the Doxy-HP-β-CD complex were selected as dependent(response)variables(Y_(1) and Y_(2)).The optimal formulation predicted by genetic algorithm(GA)combined with the models was characterized by microscopy and nuclear magnetic resonance spectrometry,and the stability of Doxy in the complex was evaluated.The highest values of Y_(1) and Y_(2) were obtained using an ANN model combined with GA which predicted the values of X_(1)-X_(4) to be 4,10.8,12 h and 25℃,respectively.The modeling and optimization results indicated that a feed-forward back-propagation ANN with one hidden layer and 10 hidden units showed better fitting to both responses compared to the RSM and SVM models.GA proved to be an efficient tool in multiobjective optimization of a pharmaceutical formulation. | Zhouhua Wang Zixin He Lei Zhang Haohao Zhang Meimei Zhang Xinguo Wen Guilan Quan Xintian Huang Xin Pan Chuanbin Wu | 2013 | Acta Pharmaceutica Sinica B2013，3，2： | 2 |
您所在的机构暂未开通此部分的功能，请联系管理员