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| 1 | Cartilage-targeting peptide-modified dual-drug delivery nanoplatform with NIR laser response for osteoarthritis therapy显示文摘Cartilage-targeting delivery of therapeutic agents is still an effective strategy for osteoarthritis(OA)therapy.Recently,scavenging for reactive oxygen species(ROS)and activating autophagy have been increasingly reported to treat OA effectively.In this study,we designed,for the first time,a dual-drug delivery system based on metal organic framework(MOF)-decorated mesoporous polydopamine(MPDA)which composed of rapamycin(Rap)loaded into the mesopores and bilirubin(Br)loaded onto the shell of MOF.The collagen II-targeting peptide(WYRGRL)was then conjugated on the surface of above nanocarrier to develop a cartilage-targeting dual-drug delivery nanoplatform(RB@MPMW).Our results indicated the sequential release of two agents from RB@MPMW could be achieved via near-infrared(NIR)laser irritation.Briefly,the rapid release of Br from the MOF shell exhibited excellent ROS scavenging ability and anti-apoptosis effects,however responsively reduced autophagy activity,to a certain extent.Meanwhile,following the NIR irradiation,Rap was rapidly released from MPDA core and further enhanced autophagy activation and chondrocyte protection.RB@MPMW continuously phosphorylated AMPK and further rescued mitochondrial energy metabolism of chondrocytes following IL-1βstimulation via activating SIRT1-PGC-1αsignaling pathway.Additionally,the cartilage-targeting property of peptide-modified nanocarrier could be monitored via Magnetic Resonance(MR)and IVIS imaging.More significantly,RB@MPMW effectively delayed cartilage degeneration in ACLT rat model.Overall,our findings indicated that the as-prepared dual-drug delivery nanoplatform exerted potent anti-inflammation and anti-apoptotic effects,rescued energy metabolism of chondrocytes in vitro and prevented cartilage degeneration in vivo,which thereby showed positive performance for OA therapy. | Song Xue Xiaojun Zhou Weilin Sang Cong Wang Haiming Lu Yiming Xu Yiming Zhong Libo Zhu Chuanglong He Jinzhong Ma | 2021 | Bioactive Materials2021，6，8： | 5 |
| 2 | Tumor cell membrane-camouflaged responsive nanoparticles enable MRI-guided immuno-chemodynamic therapy of orthotopic osteosarcoma显示文摘Osteosarcoma is a refractory bone disease in young people that needs the updating and development of effective treatment.Although nanotechnology is widely applied in cancer therapy,poor targeting and inadequate effi-ciency hinder its development.In this study,we prepared alendronate(ALD)/K7M2 cell membranes-coated hollow manganese dioxide(HMnO_(2))nanoparticles as a nanocarrier to load Ginsenoside Rh2(Rh2)for Mag-netic Resonance imaging(MRI)-guided immuno-chemodynamic combination osteosarcoma therapy.Subse-quently,the ALD and K7M2 cell membranes were successively modified on the surface of HMnO_(2) and loaded with Rh2.The tumor microenvironment(TME)-activated Rh2@HMnO_(2)-AM nanoparticles have good bone tumor-targeting and tumor-homing capabilities,excellent GSH-sensitive drug release profile and MRI capability,and attractive immuno-chemodynamic combined therapeutic efficiency.The Rh2@HMnO_(2)-AM nanoparticles can effectively trigger immunogenic cell death(ICD),activate CD4^(+)/CD8^(+)T cells in vivo,and upregulate BAX,BCL-2 and Caspase-3 in cellular level.Further results revealed that Rh2@HMnO_(2)-AM enhanced the secretion of IL-6,IFN-γand TNF-αin serum and inhibited the generation of FOXP3^(+)T cells(Tregs)in tumors.Moreover,the Rh2@HMnO_(2)-AM treatment significant restricted tumor growth in-situ tumor-bearing mice.Therefore,Rh2@HMnO_(2)-AM may serve as an effective and bio-friendly nanoparticle platform combined with immuno-therapy and chemodynamic therapy to provide a novel approach to osteosarcoma therapy. | Liwen Fu Weiying Zhang Xiaojun Zhou Jingzhong Fu Chuanglong He | 2022 | Bioactive Materials2022，7，11： | 4 |
| 3 | Electrodeposition of calcium phosphate onto polyethylene terephthalate artificial ligament enhances graft-bone integration after anterior cruciate ligament reconstruction显示文摘It is a big challenge to develop a polyethylene terephthalate(PET)artificial ligament with excellent osteogenetic activity to enhance graft-bone integration for ligament reconstruction.Herein,we evaluated the effect of biomineralization(BM)and electrodeposition(ED)method for depositing calcium-phosphate(CaP)on the PET artificial ligament in vitro and in vivo.Scanning electron microscopy and energy-dispersive X-Ray spectrometer mapping analysis revealed that the ED-CaP had more uniform particles and element distribution(Ca,P and O),and thermogravimetric analysis showed there were more CaP on the PET/ED-CaP than the PET/BM-CaP scaffold.Moreover,the hydrophilicity of PET scaffolds was significantly improved after CaP deposition.In vitro study showed that CaP coating via BM or ED method could improve the attachment and proliferation of MC3T3-E1 cells,and ED-CaP coating significantly increased osteogenic differentiation of the cells,in which the Wnt/β-catenin signaling pathway might be involved.In addition,radiological,histological and immunohistochemical results of in vivo study in a rabbit anterior cruciate ligament(ACL)reconstruction model demonstrated that the PET/BM-CaP and PET/ED-CaP scaffolds significantly improved graft-bone integration process compared to the PET scaffold.More importantly,larger areas of new bone ingrowth and the formation of fibrocartilage tissue were observed at 12 weeks in the PET/ED-CaP group,and the biomechanical tests showed increased ultimate failure load and stiffness in PET/ED-CaP group compared to PET/BM-CaP and PET group.Therefore,ED of CaP is an effective strategy for the modification of PET artificial ligament and can enhance graft-bone integration both in vitro and in vivo. | Jiangyu Cai Qianqian Zhang Jiebo Chen Jia Jiang Xiumei Mo Chuanglong He Jinzhong Zhao | 2021 | Bioactive Materials2021，6，3： | 4 |
| 4 | Construction of nanofibrous scaffolds with interconnected perfusable microchannel networks for engineering of vascularized bone tissue显示文摘Vascularization and bone regeneration are two closely related processes during bone reconstruction.A three-dimensional(3D)scaffold with porous architecture provides a suitable microenvironment for vascular growth and bone formation.Here,we present a simple and general strategy to construct a nanofibrous poly(L-lactide)/poly(ε-caprolactone)(PLLA/PCL)scaffold with interconnected perfusable microchannel networks(IPMs)based on 3D printing technology by combining the phase separation and sacrificial template methods.The regular and customizable microchannel patterns within the scaffolds(spacings:0.4 mm,0.5 mm,and 0.6 mm;diameters:0.8 mm,1 mm,and 1.2 mm)were made to investigate the effect of microchannel structure on angiogenesis and osteogenesis.The results of subcutaneous embedding experiment showed that 0.5/0.8-IPMs(spacing/diameter=0.5/0.8)and 0.5/1-IPMs(spacing/diameter=0.5/1)scaffolds exhibited more vascular network formation as compared with other counterparts.After loading with vascular endothelial growth factor(VEGF),VEGF@IPMs-0.5/0.8 scaffold prompted better human umbilical vein endothelial cells(HUVECs)migration and neo-blood vessel formation,as determined by Transwell migration,scratch wound healing,and chorioallantoic membrane(CAM)assays.Furthermore,the microangiography and rat cranial bone defects experiments demonstrated that VEGF@IPMs-0.5/0.8 scaffold exhibited better performance in vascular network formation and new bone formation compared to VEGF@IPMs-0.5/1 scaffold.In summary,our results suggested that the microchannel structure within the scaffolds could be tailored by an adjustable caramel-based template strategy,and the combination of interconnected perfusion microchannel networks and angiogenic factors could significantly enhance vascularization and bone regeneration. | Jiani Gu Qianqian Zhang Mengru Geng Weizhong Wang Jin Yang Atta ur Rehman Khan Haibo Du Zhou Sha Xiaojun Zhou Chuanglong He | 2021 | Bioactive Materials2021，6，10： | 1 |
| 5 | Novel fluorinated polymers bearing phosphonated side chains: Synthesis, characterization and properties显示文摘 | Zhu Yuanqin Chen Haihua He Chuanglong | 2011 | Journal of Polymer Research2011，18，6： | 1 |
| 6 | Preparation of core shell biodegradable microfibers for long term drug delivery 显示文摘 | Huang Huihua He Chuanglong Wang Hongsheng | 2009 | Journal of Biomedical Materials Research Part A2009，90，4： | 1 |
| 7 | Vita- min E-loaded silk fibroin nanofibrous mats fabricated by green process for skin care application显示文摘 | Xiaoyue Sheng Linpeng Fan Chuanglong He | 2013 | International Journal of Biological Macromolecules2013，56，： | 1 |
| 8 | CORRIGENDUM显示文摘On behalf of myself and each co-author, we sincerely apologize for the mistakes in the manuscript “Strontium Substituted Nanohydroxyapatite Incorporated 3D Printing Scaffold for Bone Tissue Engineering” published in Journal of Donghua University ( Eng. Ed. ) (Vol. 35, No. 1, 2018). After carefully checking the original mechanical data, we found that the stress in Fig. 5(a) was miscalculated during unit conversion process, resulting in the mistaken compressive strength and modulus in Figs. 5(b) and (c). Actually, the real stress values should be one-tenth of those in Fig. 5(a). The changes in the figure do not affect the results published in the paper. | HE Chuanglong | 2019 | Journal of Donghua University(English Edition)2019，36，4： | 0 |
| 9 | Nanofibers for the Immunoregulation in Biomedical Applications显示文摘Despite great efforts and achievement of nanomaterials in immune-associated diseases,the selection of appropriate nanomaterials and preparation technology remain some challenges and vast room for improvement.Immunotherapy has received tremendous attention throughout the medical process due to its clinical successes with the pathways of immunoactivation or immunosuppression.Recently,fibrous nanomaterials have facilitated advances in tissue repair and cancer treatments owing to the superiority of multi-channel structure,biocompatibility,tunable size and controlled surface modification.The immunoactivation-based nanofibers can potentially deliver functional agents to lesions and further actively promote immunologic intervention.On the contrary,the immunosuppression-based nanofibers prevent the immune system from overreacting through the blockage of critical pathways in vivo.This review summarizes the current application of nanofiber materials in diverse diseases,including cancer therapy,tissue regeneration(cartilage/bone,skin,tendon,nerves),myocardial infarction,psoriasis and organ defects.Some common fabrication technologies of biomedical nanofibers are also introduced.Meanwhile,the existing technical barriers and perspectives are rationally discussed,providing a constructive inspiration for the follow-up basic research and clinical transformation of nanofibers in the vibrant biomedical fields. | Liwen Fu Qian Feng Yujie Chen Jingzhong Fu Xiaojun Zhou Chuanglong He | 2022 | Advanced Fiber Materials2022，4，6： | 0 |
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