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| 1 | Construction of a cucumber genetic linkage map with SRAP markers and location of the genes for lateral branch traits显示文摘Using SRAP (sequence-related amplified polymorphism) markers a genetic linkage map of cucumber was constructed with a population consisting of 138 F2 individuals derived from a cross of the two cucumber lines, S06 and S52. In the survey of parental polymorphisms with 182 primer combinations, 64 polymorphism-revealing primer pairs were screened out, which generated totally 108 polymorphic bands with an average of 1.7 bands per primer pair and at most 6 bands from one primer pair. The constructed molecular linkage map included 92 loci, distributed in seven linkage groups and spanning 1164.2 cM in length with an average genetic distance of 12.6 cM between two neighboring loci. Based on this linkage map, the quantitative trait loci (QTL) for the lateral branch number (lbn) and the lateral branch average length (lbl) in cucumber were identified by QTLMapper1.6. A major QTL lbn1 located between ME11SA4B and ME5EM5 in LG2 could explain 10.63% of the total variation with its positively effecting allele from S06. A major QTL lbl1 located between DC1OD3 and DC1EM14 in LG2 could account for 10.38% of the total variation with its positively effecting allele from S06. | WANG Gang PAN Junsong LI Xiaozun HE Huanle WU Aizhong CAI Run | 2005 | Science China(Life Sciences)2005，48，3： | 24 |
| 2 | IL-17 response mediates acute lung injury induced by the 2009 Pandemic Influenza A (HIN1) Virus显示文摘 | Chenggang Li Penghui Yang Yang Sun Taisheng Li Chen Wang Zhong Wang Zhen Zou Yiwu Yan Wei Wang Chen Wang Zhongwei Chen Li Xing Chong Tang Xiangwu Ju Feng Guo Jiejie Deng Yan Zhao Peng Yang Jun Tang Huanling Wang Zhongpeng Zhao Zhinan Yin Bin Cao Xiliang Wang Chengyu Jiang | 2012 | Cell Research2012，22，3： | 22 |
| 3 | Chemical equilibrium controlled synthesis of polyoxymethylene dimethyl ethers over sulfated titania显示文摘The chemical equilibrium and reaction kinetic behavior in the synthesis of polyoxymethylene dimethyl ethers(DMMn) were investigated over sulfated titania in order to reveal the decisive factor controlling the reaction. The results showed that the molar ratio of adjacent DMMn products in equilibrium solution had the same value, which depended absolutely on the reaction temperature. Meanwhile, the reactions had the same DMMn products distributions under varied reaction conditions. The equilibrium constants of the related step-wise reactions for DMMn formation were equal, which were calculated based on the bulk compositions of the reaction solution. And thus, the selectivity to DMMn was mainly controlled by the chemical equilibrium, i.e., thermodynamic control. In brief, the present results provide some guidance for future synthesis of DMMn. | Huaju Li Huanling Song Feng Zhao Liwei Chen Chungu Xia | 2015 | Journal of Energy Chemistry2015，24，2： | 17 |
| 4 | Construction of a BAC library from cucumber (Cucumis sativus L.) and identification of linkage group specific clones显示文摘A bacterial artificial chromosome (BAC) library consisting of 19,200 clones with an average insert size of 105 kb has been constructed from a cucumber (Cucumis sativus L.) inbred line S94,derived from a cultivar in North China. The entire library was equivalent to approximately 5 haploid cucumber genomes. To facilitate chromosome engineering and anchor the cucumber genetic linkage map to its chromosomes,15 sequence-characterized ampli?ed regions (SCAR) and seven simple sequence repeats (SSR) markers from each link-age group of cucumber were used to screen an ordered array of pooled BAC DNA with polymerase chain reaction (PCR). Fifteen mark-ers gave at least two positive clones. As a result,22 BAC clones representing 7 linkage groups of cucumber were identified,which further validated the genome coverage and utility of the library. This BAC library and linkage group specific clones provide essential resources for future research of the cucumber genome. | Yuan Guan Qi Chen Junsong Pan Zheng Li Huanle He Aizhong Wu Rentao Song Run Cai | 2008 | Progress in Natural Science:Materials International2008，18，2： | 7 |
| 5 | The development of blood-retinal barrier during the interaction of astrocytes with vascular wall cells显示文摘Astrocytes are intimately involved in the formation and development of retinal vessels.Astrocyte dysfunction is a major cause of blood-retinal barrier injury and other retinal vascular diseases.In this study,the development of the retinal vascular system and the formation of the blood-retinal barrier in mice were investigated using immunofluorescence staining,gelatin-ink perfusion,and transmission electron microscopy.The results showed that the retinal vascular system of mice develops from the optic disc after birth,and radiates out gradually to cover the entire retina,taking the papilla optica as the center.First,the superficial vasculature is formed on the inner retinal layer; then,the vasculature extends into the inner and outer edges of the retinal inner nuclear layer,forming the deep vasculature that is parallel to the superficial vasculature.The blood-retinal barrier is mainly composed of endothelium,basal lamina and the end-feet of astrocytes,which become mature during mouse development.Initially,the naive endothelial cells were immature with few organelles and many microvilli.The basal lamina was uniform in thickness,and the glial end-feet surrounded the outer basal lamina incompletely.In the end,the blood-retinal barrier matures with smooth endothelia connected through tight junctions,relatively thin and even basal lamina,and relatively thin glial cell end-feet.These findings indicate that the development of the vasculature in the retina follows the rules of 'center to periphery' and 'superficial layer to deep layers'.Its development and maturation are spatially and temporally consistent with the functional performance of retinal neurons and photosensitivity.The blood-retinal barrier gradually becomes mature via the process of interactions between astrocytes and blood vessel cells. | Huanling Yao Tianshi Wang Jiexin Deng Ding Liu Xiaofei Li Jinbo Deng | 2014 | Neural Regeneration Research2014，9，10： | 6 |
| 6 | Rapid isolation and immune profiling of SARS-CoV-2 specific memory B cell in convalescent COVID-19 patients via LIBRA-seq显示文摘B cell response plays a critical role against SARS-CoV-2 infection.However,little is known about the diversity and frequency of the paired SARS-CoV-2 antigen-specific BCR repertoire after SARS-CoV-2 infection.Here,we performed single-cell RNA sequencing and VDJ sequencing using the memory and plasma B cells isolated from five convalescent COVID-19 patients,and analyzed the spectrum and transcriptional heterogeneity of antibody immune responses.Via linking BCR to antigen specificity through sequencing(LIBRA-seq),we identified a distinct activated memory B cell subgroup(CD11c^(high) CD95^(high))had a higher proportion of SARS-CoV-2 antigen-labeled cells compared with memory B cells.Our results revealed the diversity of paired BCR repertoire and the non-stochastic pairing of SARS-CoV-2 antigen-specific immunoglobulin heavy and light chains after SARS-CoV-2 infection.The public antibody clonotypes were shared by distinct convalescent individuals.Moreover,several antibodies isolated by LIBRA-seq showed high binding affinity against SARS-CoV-2 receptor-binding domain(RBD)or nucleoprotein(NP)via ELISA assay.Two RBD-reactive antibodies C14646P3S and C2767P3S isolated by LIBRA-seq exhibited high neutralizing activities against both pseudotyped and authentic SARS-CoV-2 viruses in vitro.Our study provides fundamental insights into B cell response following SARS-CoV-2 infection at the single-cell level. | Bing He Shuning Liu Yuanyuan Wang Mengxin Xu Wei Cai Jia Liu Wendi Bai Shupei Ye Yong Ma Hengrui Hu Huicui Meng Tao Sun Yanling Li Huanle Luo Mang Shi Xiangjun Du Wenjing Zhao Shoudeng Chen Jingyi Yang Haipeng Zhu Yusheng Jie Yuedong Yang Deyin Guo Qiao Wang Yuwen Liu Huimin Yan Manli Wang Yao-Qing Chen | 2021 | Signal Transduction and Targeted Therapy2021，6，6： | 2 |
| 7 | E3 ligase FBXW7 aggravates TMPD-induced systemic lupus erythematosus by promoting cell apoptosis显示文摘Systemic lupus erythematosus(SLE)is a systemic autoimmune disease,and the pathogenesis of SLE has not been fully elucidated.The E3 ubiquitin ligase FBXW7 has been well characterized in cancer as a tumor suppressor that can promote the ubiquitination and subsequent degradation of various oncoproteins;however,the potential role of FBXW7 in autoimmune diseases is unclear.In the present study,we identified that FBXW7 is a crucial exacerbating factor for SLE development and progression in a mouse model induced by 2,6,10,14-tetramethylpentadecane(TMPD).Myeloid cell-specific FBXW7-deficient(Lysm+FBXW7f/f)C57BL/6 mice showed decreased immune complex accumulation,glomerulonephritis,glomerular mesangial cell proliferation,and basemembrane thickness in the kidney.Lysm+FBXW7f/f mice produced fewer anti-Sm/RNP and anti-ANA autoantibodies and showed a decreased MHC II expression in B cells.In Lysm+FBXW7f/f mice,we observed that cell apoptosis was reduced and that fewer CD11b+Ly6Chi inflammatory monocytes were recruited to the peritoneal cavity.Consistently,diffuse pulmonary hemorrhage(DPH)was also decreased in Lysm+FBXW7f/f mice.Mechanistically,we clarified that FBXW7 promoted TMPD-induced cell apoptosis by catalyzing MCL1 degradation through K48-linked ubiquitination.Our work revealed that FBXW7 expression in myeloid cells played a crucial role in TMPD-induced SLE progression in mice,which may provide novel ideas and theoretical support for understanding the pathogenesis of SLE. | Zhenlu Chong Chunjing Bao Jia He Tianxiao Chen Lijia Zhong Gaopeng Li Huanle Li Lutong Fang Yinjing Song Guoxiang Fu Xuyan Yang Lihua Lai Yang Liu Qingqing Wang | 2018 | Cellular & Molecular Immunology2018，15，12： | 2 |
| 8 | Cholesterol lowering and vascular protective effects of ethanolic extract of dried fruit of Crataegus pinnatifida, hawthorn (Shan Zha), in diet-induced hypercholesterolaemic rat model显示文摘 | KWOK C Y LI Chang CHENG Huanle | 2013 | Journal of Functional Foods2013，5，3： | 1 |
| 9 | Agrobacterium tumefaciens-mediated transformation of embryogenic callus and CRISPR/Cas9-mediated genome editing in‘Feizixiao'litchi显示文摘Litchi(Litchi chinensis Sonn.)is a type of commercially prevalent subtropical and tropical fruit.Since litchi has a highly heterozygous genetic background and a long reproductive cycle,conventional breeding methods(such as hybridization)have limited ability to nurture new litchi cultivars.Here,an efficient and stable Agrobacterium tumefaciens-mediated genetic transformation of embryogenic callus was established in‘Feizixiao’litchi.Transgenic materials were verified using polymerase chain reaction(PCR)analysis,β-glucuronidase(GUS)assay,and green fluorescent protein(GFP)assay.To implement the technology of the Clustered Regularly Interspaced Short Palindromic Repeats(CRISPR)/associated protein 9(CRISPR/Cas9)technology in‘Feizixiao’litchi and verify the validity of these transformation systems,the litchi polyphenol oxidase gene(LcPPO,JF926153)was knocked out.Various categories of mutations,covering base insertions,deletions,and substitutions,were found in transgenic materials via sequence analysis.The transformation system achieved high feasibility and efficiency,and the system of CRISPR/Cas9 was successfully employed to edit genes in‘Feizixiao’litchi.This work provides an essential foundation for investigating the functions of genes and accelerating litchi genetic improvement. | Shujun Wang Guo Wang Huanling Li Fang Li Jiabao Wang | 2023 | Horticultural Plant Journal2023，9，5： | 1 |
| 10 | MicroRNA expression profile of colon cancer stem-like cells in HT29 adenocarcinoma cell line显示文摘 | Huanle Zhang Weihua Li Feifei Nan | 2011 | Biochemical and Biophysical Research Communications2011，404，： | 1 |
| 11 | Highly active/ selective heterogeneous catalyst Co/TS-1 for epoxidation of styrene by molecular oxygen:effects of catalyst preparation conditions and reaction conditions on the reaction 显示文摘 | Jiang Jian Li Rong Wang Huanling | 2008 | Catal- ysis Letters2008，120，34： | 1 |
| 12 | CRF01_AE subtype is associated with X4 tropism and fast HIV progression in Chinese patients infected through sexual transmission显示文摘 | Yijia Li Yang Han Jing Xie Lijun Gu Wenjuan Li Huanling Wang Wei Lv Xiaojing Song Yanling Li Jean-Pierre Routy Takaomi Ishida Aikichi Iwamoto Taisheng Li | 2014 | AIDS2014，，4： | 1 |
| 13 | Designed SO-/Fe203-SiO2 Solid Acids for Polyoxymethylene Dimethyl Ethers Synthesis: The Acid Sites Control and Reaction Path- ways显示文摘 | Li Huaju Song Huanling Xia Chungu | 2014 | ApplCatal B2014，165，8： | 1 |
| 14 | Determination of the refractive index and size distribution of aerosol from dual-scattering-angle optical particle counter measurements显示文摘 | Li Xuebin Zhang Yinchao | 2006 | Applied Optics2006，45，16： | 1 |
| 15 | Highly active/selective heterogeneous catalyst Co/TS-1 for epoxidation of styrene by molecular oxygen:Effects of catalyst preparation conditions and reaction conditions on the reaction显示文摘 | Jiang Jian Li Rong Wang Huanling | | 0，，3： | 1 |
| 16 | Comparative and evolutionary analysis in natural diploid and tetraploid weather loach Misgurnus anguillicaudatus based on cytochrome b sequence data in central China显示文摘 | Chengtai Yang Ling Cao Weimin Wang Yi Yang Khalid Abbas Binlun Yan Hanping Wang Li Su Yuhua Sun Huanling Wang | 2009 | Environmental Biology of Fishes2009，，1： | 1 |
| 17 | Highly active/selective heterogeneous catalyst Co/TS-1 for epoxidation of styrene by molecular oxygen:effects of catalyst preparation conditions and reaction conditions on the reaction显示文摘 | Jiang Jian Li Rong Wang Huanling | 2008 | Catalysis Letters2008，120，34： | 1 |
| 18 | Haematological characterization of loach Misgurnus anguillicaudatus : Comparison among diploid, triploid and tetraploid specimens显示文摘 | Zexia Gao Weimin Wang Khalid Abbas Xiaoyun Zhou Yi Yang James S. Diana Hanping Wang Huanling Wang Yang Li Yuhua Sun | 2007 | Comparative Biochemistry and Physiology Part A2007，，4： | 1 |
| 19 | Neuroproteetive effects of genistein and folie acid on apoptosis of rat euhured cortical neurons induced by b-amyloid 31-35 显示文摘 | YU Huanling LI Li ZHANG Xiaohong | 2009 | Br J Nutr2009，102，： | 1 |
| 20 | Safety of SARS-CoV-2 vaccines in patients with chronic myeloid leukemia: a multicenter survey in China显示文摘To the Editor:As of July 4,2021,the severe acute respiratory syndrome coronavirus-2(SARS-CoV-2)pandemic has affected>0.18 billion individuals and caused>3.9milliondeaths worldwide.ll Currently,the only method to radically overcome this pandemic is extensive vaccination.However,the clinical trials assessing most vaccines against SARS-CoV-2 have excluded patients with hematological tumors. | Yunfan Yang Yanli Zhang Qian Jiang Li Meng Weiming Li Bingcheng Liu Xiaoli Liu Li Zhou Rong Liang Xiaojian Zhu Na Xu Pu Kuang Ting Lin Huanling Zhu | 2022 | Chinese Medical Journal2022，，12： | 1 |
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