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| 1 | Hi-TOM: a platform for high-throughput tracking of mutations induced by CRISPR/Cas systems显示文摘The CRISPR/Cas system has been extensively applied to make precise genetic modifications in various organisms. Despite its importance and widespread use, large-scale mutation screening remains time-consuming, labour-intensive and costly. Here, we developed Hi-TOM(available at http://www.hi-tom.net/hi-tom/), an online tool to track the mutations with precise percentage for multiple samples and multiple target sites. We also described a corresponding next-generation sequencing(NGS) library construction strategy by fixing the bridge sequences and barcoding primers. Analysis of the samples from rice, hexaploid wheat and human cells reveals that the Hi-TOM tool has high reliability and sensitivity in tracking various mutations, especially complex chimeric mutations frequently induced by genome editing. Hi-TOM does not require special design of barcode primers,cumbersome parameter configuration or additional data analysis. Thus, the streamlined NGS library construction and comprehensive result output make Hi-TOM particularly suitable for high-throughput identification of all types of mutations induced by CRISPR/Cas systems. | Qing Liu Chun Wang Xiaozhen Jiao Huawei Zhang Lili Song Yanxin Li Caixia Gao Kejian Wang | 2019 | Science China(Life Sciences)2019，62，1： | 37 |
| 2 | Tobacco RING E3 Ligase NtRFP1 Mediates Ubiquitination and Proteasomal Degradation of a Geminivirus-Encoded βC1显示文摘西红柿黄叶卷屈中国联系病毒的 betasatellite 编码的 C1 蛋白质作为一个致病力决定因素工作。更好理解 C1 在致病力工作的分子的基础,酵母一个烟草 cDNA 图书馆的二混血儿的屏幕作为诱饵用 C1 被执行。屏幕表明 C1 与烟草戒指手指蛋白质交往指明了 NtRFP1,它被双分子的荧光互补和 co-immunoprecipitation 试金进一步在 Nicotiana benthamiana 房间证实。NtRFP1 的表示被 C1 导致,并且在 vitro ubiquitination,试金证明 NtRFP1 是调停的功能的 E3 ubiquitin ligase C1 ubiquitination。另外, C1 被显示是在 vivo 的 ubiquitinated 并且由植物 26S proteasome 降级。在病毒的感染以后,植物 overexpressing NtRFP1 开发了稀释症状,而有 NtRFP1 的 silenced 表示的植物显示出严重症状。证据的另外的线证明 NtRFP1 通过由 26S proteasome 支持它的降级稀释导致 C1 的症状。一起拿,我们的结果建议烟草戒指 E3 ligase NtRFP1 由与 C1 交往调停稀释疾病症状它经由 ubiquitin/26S proteasome 系统的 ubiquitination 和降级。 | Qingtang Shen Tao Hu Min Bao Linge Cao Huawei Zhang Fengmin Song Qi Xie Xueping Zhou | 2016 | Molecular Plant2016，9，6： | 8 |
| 3 | Adaptive Service Provisioning for Mobile Edge Cloud显示文摘A mobile edge cloud provides a platform to accommodate the offloaded traffic workload generated by mobile devices.It can significantly reduce the access delay for mobile application users.However,the high user mobility brings significant challenges to the service provisioning for mobile users,especially to delay-sensitive mobile applications.With the objective to maximize a profit,which positively associates with the overall admitted traffic served by the local edge cloud,and negatively associates with the access delay as well as virtual machine migration delay,we study a fundamental problem in this paper:how to update the service provisioning solution for a given group of mobile users.Such a profit-maximization problem is formulated as a nonlinear integer linear programming and linearized by absolute value manipulation techniques.Then,we propose a framework of heuristic algorithms to solve this Nondeterministic Polynomial(NP)-hard problem.The numerical simulation results demonstrate the efficiency of the devised algorithms.Some useful summaries are concluded via the analysis of evaluation results. | HUANG Huawei GUO Song | 2017 | ZTE Communications2017，15，2： | 5 |
| 4 | Retro-enantio isomer of angiopep-2 assists nanoprobes across the blood-brain barrier for targeted magnetic resonance/fluorescence imaging of glioblastoma显示文摘Glioblastoma(GBM),one of the most common primary intracranial malignant tumours,is very difficult to be completely excised by surgery due to its irregular shape.Here,we use an MRI/NIR fluorescence dual-modal imaging nanoprobe that includes superparamagnetic iron oxide nanoparticles(SPIONs)modified with indocyanine(Cy7)molecules and peptides(ANG orDANG)to locate malignant gliomas and guide accurate excision.Both peptides/Cy7-SPIONs probes displayed excellent tumour-homing properties and barrier penetrating abilities in vitro. | Ruoxi Xie Zijun Wu Fanxin Zeng Huawei Cai Dan Wang Lei Gu Hongyan Zhu Su Lui Gang Guo Bin Song Jinxing Li Min Wu Qiyong Gong | 2021 | Signal Transduction and Targeted Therapy2021，6，9： | 3 |
| 5 | Determining gastric cancer resectability by dynamic MDCT显示文摘 | Zilai Pan Huan Zhang Chao Yan Lianjun Du Bei Ding Qi Song Huawei Ling Baisong Huang Kemin Chen | 2010 | European Radiology2010，，3： | 2 |
| 6 | pHsh vectors, a novel expression system of Escherichia coli for the large-scale production of recombinant enzymes显示文摘 | Huawei Wu Jianjun Pei Yu Jiang Xin Song Weilan Shao | 2010 | Biotechnology Letters2010，，6： | 1 |
| 7 | Evalua- tion of time-varying availability in multi echelon spare parts sys- tems with passivation显示文摘 | Hoong Chuin Lau Huawei Song Chuen Teck See | 2006 | European Journal of Operational Re- search2006，170，1： | 1 |
| 8 | Evaluation of time-varying availability in multi-echelon spare parts systems with passivation 显示文摘 | Hoong Chuin Lau Huawei Song Chuen Teck See | 2006 | European Journal of Operational Research2006，170，1： | 1 |
| 9 | Evaluation of time-varying availability in multi-echelon spare parts systems with passivation显示文摘 | Hoong Chuin Lau Huawei Song Chuen Teck See Siew Yen Cheng | 2004 | European Journal of Operational Research2004，，1： | 1 |
| 10 | Study on Fe 3 O 4 /polyaniline electromagnetic composite hollow spheres prepared against sulfonated polystyrene colloid template显示文摘 | Huifang Xu Haijiao Zhang Tong Lv Huawei Wei Fei Song | 2013 | Colloid and Polymer Science2013，，7： | 1 |
| 11 | Biocompatibility of Polypropylene Non-woven Fabric Membrane via UV-induced Graft Polymerization of 2-acrylamido-2methylpropane Sulfonic Acid 显示文摘 | Song Lingjie Zhao Jie Yang Huawei | 2011 | Applied Surface Science2011，258，1： | 1 |
| 12 | Gas separation performance of C/CMS membranes derived from poly(furfuryl alcohol)(PFA)with different chemical structure显示文摘 | SONG Chengwen WANG Tonghua JIANG Huawei | 2010 | Journal of Membrane Science2010，361，： | 1 |
| 13 | Novel heterozygous USH1C mutation impacts hair cell mechanotransduction and causes progressive hearing loss显示文摘Hearing loss is one of the most common sensory disorders worldwide,affecting approximately 466 million people,including 34 million children[1].Genetic mutations accounts for approximately 60%of inherited hearing loss cases[2,3].These genetic changes result in a wide variety of clinical manifestations,ranging from nonsyndromic hearing loss(NSHL)to over 400 syndromes involving hearing loss and from mild adult-onset hearing loss to profound congenital deafness,covering the entire spectrum of Mendelian inheritance. | Yanyan Jia Gaogan Jia Luo Guo Nan Song Yu-Meng Wang Luoying Jiang Yilai Shu Yan Chen Shujia Zhu Huawei Li Wenyan Li | 2024 | Science Bulletin2024，69，2： | 0 |
| 14 | Hybrid solid electrolyte interphases formed in conventional carbonate electrolyte enable high-voltage and ultra-stable magnesium metal batteries显示文摘Magnesium metal batteries are considered as viable alternatives of lithium-ion batteries for their low cost and high capacity of magnesium.Nevertheless,the practical application of magnesium metal batteries is extremely challenging due to a lack of suitable electrolyte that can stabilize magnesium metal anode and high-voltage cathode simultaneously.Herein,we found that in-situ formed lithium/magnesium hybrid electrolyte interphases in conventional LiPF6-containing carbonate-based electrolyte can not only prevent the production of passivation layer on the magnesium metal anode,but also inhibit the oxidation of the electrolyte under high voltage.The symmetric magnesium‖magnesium battery can achieve reversible stripping/plating for 1600 and 600 h at 0.02 and 0.1 mA cm^(-2),respectively.In addition,when coupled with a carbon fiber cathode,the magnesium metal battery exhibited a capacity retention rate of 96.3% for 1000 cycles at a current density of 500 mA g^(-1)and presented a working voltage of ~3.1 V.This research paves a new and promising path to the commercialization process of rechargeable magnesium metal batteries. | Yong Xie Huawei Song Siyang Ye Fei Tian Junjie Xie Danni Lei Chengxin Wang | 2023 | Journal of Energy Chemistry2023，，3： | 0 |
| 15 | Fine-grained sequence-to-sequence lip reading based on self-attention and self-distillation显示文摘1 Introduction The lip reading involves converting the image sequence into the corresponding text sequence.Currently,lip reading has significant applications in many fields,such as assisted speech recognition,helping the speech impaired.Lip reading belongs to fine-grained video analysis and requires the local information and the overall spatial information of sequence.Most existing approaches capture local spatial information with CNN and temporal information with RNN generally.Considering these general methods,we propose a fine-grained method based on self-attention and self-distillation.The whole model mainly includes the CNN front-end,pixel-wise learning,temporal learning,and decoder.Specifically,we apply the CNN front-end to capture shallow spatial features inside the image sequence,and employ the Resformer module including self-attention to learn the global spatial correlation between pixels,namely,pixel-wise learning. | Junxiao XUE Shibo HUANG Huawei SONG Lei SHI | 2023 | Frontiers of Computer Science2023，17，6： | 0 |
| 16 | Artificial Hybrid Solid Electrolyte-Mediated Ca Metal for Ultradurable Room Temperature 5 V Calcium Batteries显示文摘The anodic stability and reversibility of Ca metal electrodeposition/dissolution have always been hampered by surface passivation and anion corrosion,especially in F-based carbonate ester electrolytes at high device voltages.To avoid direct Ca metal/electrolyte reactions,an artificial hybrid solid electrolyte layer(AHSEL),which is characteristic of sodium/calcium carbonate and calcium hydride nitride nanocrystals of size<10 nm encapsulated by amorphous C,N species,is constructed on calcium with good ion conductivity(≈0.01 mS cm^(-1))and uniform coating of thickness≈20μm.After cycling in the KPF_(6) electrolyte,AHSEL is transformed into Na/K/Ca hybrid solid electrolyte interphases(SEIs),which is a compact layer composed of monodisperse nanocrystals(mostly Ca_(2)NH)and small amorphous zones,thus greatly suppressing the fluoridation of the Ca deposit.Consequently,the plating/stripping performance of AHSEL-modified Ca(AHSEL-Ca)is markedly improved compared with that of pristine Ca,lasting for>1400 h at a polarization shift of<0.4 mV/h.The AHSEL-Ca anode also endows Ca batteries with superior anodic stability with a ceiling voltage of up to 5.0 V,a high discharge voltage(>3.3 V),a large capacity of≈80 mAh g^(-1)at 200 mA g^(-1),and an ultralong lifespan≈5000 cycles. | Huawei Song Chengxin Wang | 2023 | Energy & Environmental Materials2023，6，2： | 0 |
| 17 | Reconfigurable Vortex-like Paramagnetic Nanoparticle Swarm with Upstream Motility and High Body-length Ratio Velocity显示文摘Drug delivery systems with high-targeted doses can minimize excipients,reduce side effects,and improve efficacy.Human blood circulation is a complex circulatory system,and the motion control of microrobots in the static flow field in vitro is completely different from in vivo.How to achieve precise counterflow motion for targeted drug delivery without vascular blockage and immune rejection is the biggest challenge for micro-nano robots.Here,we propose a control method that enables vortex-like paramagnetic nanoparticle swarm(VPNS)to move upstream against the flow.By mimicking the clustering motion of wild herring schools and the rolling of leukocytes,VPNS are incredibly stable even when subjected to high-intensity jet impacts in the blood environment,can travel upstream,anchor at the target location,and dissipate when the magnetic field is withdrawn,which greatly reduces the risk of thrombosis.VPNS can also upstream along the vessel wall without an additional energy source and has a marked targeted therapeutic effect on subcutaneous tumors. | Luyao Wang Han Gao Hongyan Sun Yiming Ji Li Song Lina Jia Chutian Wang Chan Li Deyuan Zhang Ye Xu Huawei Chen Lin Feng | 2023 | Research2023，，4： | 0 |
| 18 | Low-bias,high-photoresponsivity SnSe2 nanofilm with an Au split-ring array-based THz detector toward 6G communication显示文摘6G technology with the four characteristics of an intelligent endogenous,secure endogenous,multi-domain convergence,and integrated computing network,will help society toward an intelligent era[1].The low power consumption and high photoresponsivity of 6G core devices,and particularly the improvement of detector performance. | Qi SONG Zhiwen ZHOU Yifei XU Min ZHANG Junbo YANG Huawei LIANG Tianwu WANG Peiguang YAN | 2023 | Science China(Information Sciences)2023，66，6： | 0 |
| 19 | Solid-state reaction induced defects in multi-walled carbon nanotubes for improving microwave absorption properties显示文摘Dielectric polarization performance induced by defect engineering approach has been proved to be an effective way for improving the microwave absorption property of carbon-based materials.Assisting by the solid-state reaction,the structural integrity of multi-walled carbon nanotubes(MWCNTs)would be broken along with the volume expansion and etching process of cobalt oxides.Therefore,the defects could be obtained and result in the enhancement of microwave absorption property.Ascribing to the broken wall structures,the defect-distances(L_(D))and concentrations(n_(D))have been optimized to be 9.80 nm and3.37×10^(11) cm^(-2).The minimum reflection loss(RL)had reached-54.6 dB at 4.5 GHz with a thickness of4.13 mm and the corresponding effective absorption bandwidth(EAB_(<-10 dB))was analyzed to be 14.6 GHz.Such enhancement is correlated to the dielectric polarization and the permeability-to-permittivity transformation raised from the defect structures and concentrations.The present work demonstrates an effective strategy for tailoring the microwave absorption property of MWCNTs by engineering defect concentrations,and could be further extended to other carbon-based absorbents. | Tong Gao Zhengyu Zhang Yixing Li Yujuan Song Huawei Rong Xuefeng Zhang | 2022 | Journal of Materials Science & Technology2022，，13： | 0 |
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