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| 1 | Rescue of premature aging defects in Cockayne syndrome stem cells by CRISPR/Cas9-mediated gene correction显示文摘Cockayne syndrome(CS)is a rare autosomal recessive inherited disorder characterized by a variety of clinical features,including increased sensitivity to sunlight,progressive neurological abnormalities,and the appearance of premature aging.However,the pathogenesis of CS remains unclear due to the limitations of current disease models.Here,we generate integration-free induced pluripotent stem cells(iPSCs)from fibroblasts from a CS patient bearing mutations in CSB/ERCC6 gene and further derive isogenic genecorrected CS-iPSCs(GC-iPSCs)using the CRISPR/Cas9 system.CS-associated phenotypic defects are recapitulated in CS-iPSC-derived mesenchymal stem cells(MSCs)and neural stem cells(NSCs),both of which display increased susceptibility to DNA damage stress.Premature aging defects in CS-MSCs are rescued by the targeted correction of mutant ERCC6.We next map the transcriptomic landscapes in CS-iPSCs and GC-iPSCs and their somatic stem cell derivatives(MSCs and NSCs)in the absence or presence of ultraviolet(UV)and replicative stresses,revealing that defects in DNA repair account for CS pathologies.Moreover,we generate autologous GC-MSCs free of pathogenic mutation under a cGMP(Current Good Manufacturing Practice)-compliant condition,which hold potential for use as improved biomaterials for future stem cell replacement therapy for CS.Collectively,our models demonstrate novel disease features and molecular mechanisms and lay a foundation for the development of novel therapeutic strategies to treat CS. | Si Wang Zheying Min Qianzhao Ji Lingling Geng Yao Su Zunpeng Liu Huifang Hu Lixia Wang Weiqi Zhang Keiichiro Suzuiki Yu Huang Puyao Zhang Tie-Shan Tang Jing Qu Yang Yu Guang-Hui Liu Jie Qiao | 2020 | Protein & Cell2020，11，1： | 7 |
| 2 | Coking-resistant Ni-ZrO_2/Al_2O_3 catalyst for CO methanation显示文摘Highly coke-resisting Zr O2-decorated Ni/Al2O3 catalysts for CO methanation were prepared by a two-step process. The support was first loaded with Ni O by impregnating method and then modified with Zr O2 by deposition-precipitation method(IM-DP). Nitrogen adsorptiondesorption, X-ray diffraction, scanning electron microscopy, transmission electron microscopy, thermogravimetric analysis, H2 temperatureprogrammed reduction and desorption, NH3temperature-programmed desorption, and zeta potential analysis were employed to characterize the samples. The results revealed that, compared with the catalysts with the same composition prepared by co-impregnation(CI) and sequential impregnation(SI) methods, the Ni/Al2O3 catalyst prepared by IM-DP showed much enhanced catalytic performance for syngas methanation under the condition of atmospheric pressure and a high weight hourly space velocity of 120000 m L g-1 h-1. In a 80 h life time test under the condition of 300–600°C and 3.0 MPa, this catalyst showed high stability and resistance to coking, and the amount of deposited carbon was only 0.4 wt%. On the contrary, the deposited carbon over the catalyst without Zr O2 reached 1.5 wt% after a 60 h life time test. The improved catalytic performance was attributed to the selective deposition of Zr O2 nanoparticles on the surface of Ni O rather than Al2O3, which could be well controlled via changing the electrostatic interaction in the DP procedure. This unique structure could enhance the dissociation of CO2 and generate surface oxygen intermediates, thus preventing carbon deposition on the Ni particles in syngas methanation. | Qing Liu Fangna Gu Jiajian Gao Huifang Li Guangwen Xu Fabing Su | 2014 | Journal of Energy Chemistry2014，23，6： | 4 |
| 3 | Fen1 mutations that specifically disrupt its interaction ~vith PCNA cause aneuploidy-associated cancer显示文摘 | Li Zheng Huifang Dai Muralidhar L Hegde Mian Zhou Zhigang Guo Xiwei Wu Jun Wu Lei Su Xueyan Zhong Sankar Mitra Qin Huang Kemp H Kernstine Gerd P Pfeifer Binghui Shen | 2011 | Cell Research2011，21，7： | 3 |
| 4 | GFAP-Positive Progenitor Cell Production is Concentrated in Specific Encephalic Regions in Young Adult Mice显示文摘Previous genetic fate-mapping studies have indicated that embryonic glial fibrillary acidic proteinpositive(GFAP^+) cells are multifunctional progenitor/neural stem cells that can produce astrocytes as well as neurons and oligodendrocytes throughout the adult mouse central nervous system(CNS). However, emerging evidence from recent studies indicates that GFAP^+cells adopt different cell fates and generate different cell types in different regions. Moreover, the fate of GFAP^+cells in the young adult mouse CNS is not well understood. In the present study, hGFAP-Cre/R26 R transgenic mice were used to investigate the lineage of embryonic GFAP^+cells in the young adult mouse CNS. At postnatal day 21, we found that GFAP^+cells mainly generated NeuN^+neurons in the cerebral cortex(both ventral and dorsal), hippocampus, and cerebellum. Strangely, these cells were negative for the Purkinje cell marker calbindin in the cerebellum and the neuronal marker NeuN in the thalamus. Thus,contrary to previous studies, our genetic fate-mapping revealed that the cell fate of embryonic GFAP^+cells at the young adult stage is significantly different from that at the adult stage. | Zhibao Guo Yingying Su Huifang Lou | 2018 | Neuroscience Bulletin2018，34，5： | 3 |
| 5 | Trafficking pathway between plasma membrane and mitochondria via clathrin-mediated endocytosis显示文摘Endocytosis is a basic cellular process that describes a form of active transport across the plasma membrane into the cell.The endocytic pathway consists of distinct membrane compartments;internalized molecules are delivered to early endosomes,and some of them are recycled back to the surface,whereas other molecules are sent to late endosomes and lysosomes for degradation.However,little is known about how mitochondria are involved in the endocytic pathway.Here,we report that FM dyes, membrane-impermeant fluorescent lipid probes,can traffic to mitochondria directly from the plasma membrane by clathrinmediated endocytosis.FM dye entry into mitochondria uses microtubule-dependent active transport,but the mechanism is different from the classical endocytic pathway.Hence,this study reveals a previously unrealized lipid trafficking pathway from the plasma membrane to mitochondria. | Zhongya Wei Wenfeng Su Huifang Lou Shumin Duan Gang Chen | 2018 | Journal of Molecular Cell Biology2018，10，6： | 2 |
| 6 | Functional markers developed from TaGS3, a negative regulator of grain weight and size, for marker-assisted selection in wheat显示文摘The TaGS3 homoeologous genes(homoeologs)located on chromosomes 7 A,4 A,and7 D in hexaploid wheat were cloned.Relative expression analysis of the three Ta GS3 homoeologs revealed that the expression levels of TaGS3-4 A and TaGS3-7 D in developing grains were higher than that of TaGS3-7 A.Genetic evidence showed that Ta GS3 was a negative regulator of grain weight and grain size.Fifteen polymorphic sites and five haplotypes were detected in TaGS3-4 A.Two molecular markers were developed to distinguish the five haplotypes.Association analysis using 260 accessions from Chinese wheat mini-core collection(MCC)indicated that TaGS3-4 A affected thousand grain weight(TGW)and grain length(GL).HAP-4 A-1 and HAP-4 A-2 were favorable haplotypes that increased TGW and GL and had undergone strong selection during domestication of wheat.In addition,interaction of the TaGS3-4 A and TaGS3-7 D homoeologs had significant additive effects on the grain traits.Hap-4 A-1/Hap-7 D-2 was the best haplotype combination in increasing TGW and GL.The frequencies and geographic distributions of favorable TaGS3 haplotypes among 1388 wheat accessions from worldwide sources provided clues for selection of yield-related traits.Our findings demonstrated that TaGS3-4 A had significant effects on TGW and GL.Marker-assisted selection of HAP-4 A-1/2 combined with HAP-7 D-2 has potential to increase wheat yields. | Wei Zhang Huifang Li Liya Zhi Qiannan Su Jiajia Liu Xiaoli Ren Deyuan Meng Na Zhang Jun Ji Xueyong Zhang Junming Li | 2020 | The Crop Journal2020，8，6： | 2 |
| 7 | Single-cell transcriptomic profiling unravels the adenoma-initiation role of protein tyrosine kinases during colorectal tumorigenesis显示文摘The adenoma-carcinoma sequence is a well-accepted roadmap for the development of sporadic colorectal cancer.However,cellular heterogeneity in aberrant epithelial cells limits our understanding of carcinogenesis in colorectal tissues.Here,we performed a single-cell RNA sequencing survey of 54,788 cells from patient-matched tissue samples,including blood,normal tissue,para-cancer,polyp,and colorectal cancer. | Xiaobo Zheng Jinen Song Chune Yu Zongguang Zhou Xiaowei Liu Jing Yu Guangchao Xu Jiqiao Yang Xiujing He Xin Bai Ya Luo Yu Bao Huifang Li Lie Yang Mingqing Xu Nan Song Xiaodong Su Jie Xu Xuelei Ma Hubing Shi | 2022 | Signal Transduction and Targeted Therapy2022，7，3： | 2 |
| 8 | An insight into aggregation kinetics of polystyrene nanoplastics interaction with metal cations显示文摘Once inevitably released into the aquatic environment,polystyrene nanoplastics(PS-NPs)will present complicated environmental behaviors,of which the aggregation is a key process determining their environmental fate and impact.In this study,the aggregation kinetics of different sizes(30 nm and 100 nm)of PS-NPs with metal cations(Na^(+),K^(+),Ca^(2+),Mg^(2+)and Pb^(2+))at different solution pH(3,6 and 8)were investigated.The results showed that the aggregation of PS-NPs increased with cation concentration.Taking Pb^(2+)as an example,the adsorption behavior of cations onto PS-NPs was determined by transmission electron microscopy(TEM)and energy dispersive X-ray(EDX)spectroscopy,which demonstrated Pb^(2+)could be adhered onto the surface of PS-NPs with the effect of charge neutralization.The critical coagulation concentrations(CCC)of smaller PS-NPs were higher than that of larger PS-NPs for monovalent cations,whereas a different pattern is observed for divalent cations.It was suggested that there were other factors that DLVO theory does not consider affect the stability of NPs with different particle sizes.In addition,it should be noted that PS-NPs had the capacity of adsorbing large amounts of heavy metal cations and carried them transport to a long distance,and the corresponding ecological risks need to further elucidate. | Yucheng Zhang Xiaotong Su Nora FYTam Xiaolan Lao Meiling Zhong Qihang Wu Huifang Lei Zihui Chen Zhang Li Jie Fu | 2022 | Chinese Chemical Letters2022，33，12： | 1 |
| 9 | Synthesis and red luminescence of Pr3+ -doped CaTiO3 nanophosphor from polymer precursor显示文摘 | Pan Yuexiao Su Qiang Xu Huifang | 2003 | J Solid Stat Chem2003，174，： | 1 |
| 10 | Synthesis and Red Luminescence of Pr^3+ -doped CaTiO3 Nanophosphor From Polymer Precursor 显示文摘 | PAN Yuexiao SU Qiang XU Huifang | 2003 | J Solid State Chem2003，174，： | 1 |
| 11 | Crosstalk between IL-15Rα^(+)tumor-associated macrophages and breast cancer cells reduces CD8+T cell recruitment显示文摘Background:Interleukin-15(IL-15)is a promising immunotherapeutic agent owing to its powerful immune-activating effects.However,the clinical benefits of these treatments are limited.Crosstalk between tumor cells and immune cells plays an important role in immune escape and immunotherapy drug resistance.Herein,this study aimed to obtain in-depth understanding of crosstalk in the tumor microenvironment for providing potential therapeutic strategies to prevent tumor progression.Methods:T-cell killing assays and co-culture models were developed to determine the role of crosstalk between macrophages and tumor cells in breast cancer resistant to IL-15.Western blotting,histological analysis,CRISPR-Cas9 knockout,multi-parameter flow cytometry,and tumor cell-macrophage co-injection mouse models were developed to examine the mechanism by which IL-15Rα^(+)tumor-associated macrophages(TAMs)regulate breast cancer cell resistance to IL-15.Results:We found thatmacrophages contributed to the resistance of tumor cells to IL-15,and tumor cells induced macrophages to express high levels of theαsubunit of the IL-15 receptor(IL-15Rα).Further investigation showed that IL-15Rα^(+)TAMs reduced the protein levels of chemokine CX3C chemokine ligand 1(CX3CL1)in tumor cells to inhibit the recruitment of CD8^(+)T cells by releasing the IL-15/IL-15Rαcomplex(IL-15Rc).Administration of an IL-15Rc blocking peptide markedly suppressed breast tumor growth and overcame the resistance of cancer cells to anti-programmed cell death protein 1(PD-1)antibody immunotherapy.Interestingly,Granulocyte-macrophage colony-stimulating factor(GMCSF)inducedγchain(γc)expression to promote tumor cell-macrophage crosstalk,which facilitated tumor resistance to IL-15.Additionally,we observed that the non-transcriptional regulatory function of hypoxia inducible factor-1alpha(HIF-1α)was essential for IL-15Rc to regulateCX3CL1 expression in tumor cells.Conclusions:The IL-15Rc-HIF-1α-CX3CL1 signaling pathway serves as a crosstalk between macrophages and tumor cells in the tumormicroenvironment of breast cancer.Targeting this pathway may provide a potential therapeutic strategy for enhancing the efficacy of cancer immunotherapy. | Wenlong Zhang Qing Zhang Nanfei Yang Qian Shi Huifang Su Tingsheng Lin Zhonglei He Wenxin Wang Hongqian Guo Pingping Shen | 2022 | Cancer Communications2022，42，6： | 0 |
| 12 | Correction to:Rescue of premature aging defects in Cockayne syndrome stem cells by CRISPR/Cas9-mediated gene correction显示文摘CORRECTION TO:PROTEIN CELL HTTPS://DOI.ORG/10.1007/S13238-019-0623-2 In Fig.7C,we used the ERCC6mut.iPSCs(CS iPSCs)as NANOG positive control pluripotent cells in the upper pan-els.However,these cells were inadvertently labeled as ERCC6^(GC)-iPSCs.In the revised version of Fig.7C,we have updated the high-quality images along with the corrected mark.In addition,we have also made corresponding chan-ges in the figure legend. | Si Wang Zheying Min Qianzhao Ji Lingling Geng Yao Su Zunpeng Liu Huifang Hu Lixia Wang Weiqi Zhang Keiichiro Suzuiki Yu Huang Puyao Zhang Tie-Shan Tang Jing Qu Yang Yu Guang-Hui Liu Jie Qiao | 2022 | Protein & Cell2022，13，8： | 0 |
| 13 | Chemo-selective control of Ritter-type reaction by coordinatively unsaturated inorganic salt hydrates显示文摘The Ritter-type reaction, without relying on a pre-installed functional group, is a highly efficient tool forthe construction of amides. However, the intrinsic chemo-selectivity that determines the generation ofamides or byproducts limits the efficiency and yield of the reaction. From 68 different types of hydrates,we found that a coordinatively unsaturated inorganic salt hydrate, MgSO_(4)·2H_(2)O, controlled chemoselectivity and eliminated the shortcomings of other synthesis approaches. To rationalize the differencesin selectivity of inorganic salt hydrates, we analyzed their corresponding water content, alkalinity, anions,and cations. MgSO_(4)·2H_(2)O was used with diverse scaffolds and C–H oxygenations, which demonstratedits generality in synthetic utility. Because it is readily available and it significantly improves yield, we expectthat MgSO_(4)·2H_(2)O will have broad application for Ritter-type reactions. | Huifang Lai Jiexin Xu Jin Lin Biling Su Daijun Zha | 2022 | Organic Chemistry Frontiers2022，9，6： | 0 |
| 14 | Study of an Integrated River Basin Management Model and Its Implications for River and Lake Management in China显示文摘A model combining basin management and administrative management is currently adopted for the lake and river management in China. In applications,the model suffers many problems,including the confusion of rights and responsibilities of river basin authorities and the dominant governmental agencies,and the lack of effective public participation. In this paper,we employ the Great Lakes Basin in the United States,the Loire-Brittany River Basin in France,the Southeast Basin in the United Kingdom,the Murray-Darling Basin in Australia,and general German model as examples to analyze and summarize the characteristics of their river and lake management modes,regarding river basin authorities,dominant governmental agencies,public participation,and basin management. This study may provide some referable experience for the integrated river basin management in China. | Xu Huifang Wang Su | 2017 | Meteorological and Environmental Research2017，8，4： | 0 |
| 15 | A dense graphene monolith with poloxamer prefunctionalization enabling aqueous redispersion to obtain solubilized graphene sheets显示文摘The realization of good aqueous dispersibility of commercial graphene products composed of exfoliated graphene sheets is of significance for downstream applications.However,the tap density of commercial graphene powder is quite low(0.03-0.1 kg/m3),meaning that 1 kg graphene powder occupies about 10-30 m3 in volume during transportation.And,the available content of commercial graphene dispersion/slurry in aqueous medium cannot exceed 5 wt%,although the density is high(≈1050 kg/m3).In this work,a graphene monolith was prepared by oven-drying of graphene sheets prefunctionalized with poloxamer surfactants.Our graphene monoliths not only have a high density(1500 kg/m^3) and high graphene content(≈10 wt%),but also a full capability to be completely redispersed(≈100%) in water by bath sonication to obtain solubilized graphene sheets,whose lateral size and thickness are unchanged compared to as-exfoliated ones.Moreover,a simple empirical method was proposed to predict the redispersion capability of graphene monoliths using different poloxamers by contact angle measurements.Our results provide a universal approach to make exfoliated graphene-based products with better downstream availability and lower transportation cost. | Chen Ye Fan Zhang Xue Tan Huifang Sun Wen Dai Ke Yang Minghui Yang Shiyu Du Dan Dai Jinhong Yu Nan Jiang Weitao Su Li Fu He Li Jing Kong Cheng-Te Lin | 2020 | Chinese Chemical Letters2020，31，9： | 0 |
| 16 | Inosine:A broad-spectrum anti-inflammatory against SARS-CoV-2 infection-induced acute lung injury via suppressing TBK1 phosphorylation显示文摘Severe acute respiratory syndrome coronavirus 2(SARS-CoV-2)-induced cytokine storms constitute the primary cause of coronavirus disease 19(COVID-19)progression,severity,criticality,and death.Glucocorticoid and anti-cytokine therapies are frequently administered to treat COVID-19,but have limited clinical efficacy in severe and critical cases.Nevertheless,the weaknesses of these treatment modalities have prompted the development of anti-inflammatory therapy against this infection.We found that the broad-spectrum anti-inflammatory agent inosine downregulated proinflammatory interleukin(IL)-6,upregulated anti-inflammatory IL-10,and ameliorated acute inflammatory lung injury caused by multiple infectious agents.Inosine significantly improved survival in mice infected with SARS-CoV-2.It indirectly impeded TANK-binding kinase 1(TBK1)phosphorylation by binding stimulator of interferon genes(STING)and glycogen synthase kinase-3β(GSK3β),inhibited the activation and nuclear translocation of the downstream transcription factors interferon regulatory factor(IRF3)and nuclear factor kappa B(NF-κB),and downregulated IL-6 in the sera and lung tissues of mice infected with lipopolysaccharide(LPS),H1N1,or SARS-CoV-2.Thus,inosine administration is feasible for clinical anti-inflammatory therapy against severe and critical COVID-19.Moreover,targeting TBK1 is a promising strategy for inhibiting cytokine storms and mitigating acute inflammatory lung injury induced by SARS-CoV-2 and other infectious agents. | Ningning Wang Entao Li Huifang Deng Lanxin Yue Lei Zhou Rina Su Baokun He Chengcai Lai Gaofu Li Yuwei Gao Wei Zhou Yue Gao | 2023 | Journal of Pharmaceutical Analysis2023，13，1： | 0 |
| 17 | The Application of Research Study in Higher Vocational Medical College Teaching显示文摘 | Su Yanhong Zhu Huifang Lei Ming | 2014 | International Journal of Technology Management2014，，3： | 0 |
| 18 | Engineering oxygen vacancies and localized amorphous regions in CuO-ZnO separately boost catalytic reactivity and selectivity显示文摘Generating different types of defects in heterogeneous catalysts for synergetic promotion of the reactivity and selectivity in catalytic reactions is highly challenging due to the lack of effective theoretical guidance.Herein,we demonstrate a facile strategy to introduce two types of defects into the CuO-ZnO model catalyst,namely oxygen vacancies(OVs)induced by H2 partial reduction and localized amorphous regions(LARs)generated via the ball milling process.Using industrially important Rochow–Müller reaction as a representative,we found OVs predominantly improved the target product selectivity of dimethyldichlorosilane,while LARs significantly increased the conversion of reactant Si.The CuO-ZnO catalyst with optimized OVs and LARs contents achieved the best catalytic property.Theoretical calculation further revealed that LARs promote the generation of the Cu3Si active phase,and OVs impact the electronic structure of the Cu3Si active phase.This work provides a new understanding of the roles of different catalyst defects and a feasible way of engineering the catalyst structure for better catalytic performances. | Yongjun Ji Xiaoli Chen Shaomian Liu Liwen Xing Xingyu Jiang Bin Zhang Huifang Li Wenxing Chen Ziyi Zhong Ligen Wang Guangwen Xu Fabing Su | 2023 | Nano Research2023，16，2： | 0 |
| 19 | Facile synthesis of ultrabright luminogens with specific lipid droplets targeting feature for in vivo two-photon fluorescence retina imaging显示文摘The application of fluorescent probes for in vivo retinal imaging is of great importance,which could provide direct and crucial imaging evidence for a better understanding of common eye diseases.Herein,a group of bright organic luminogens with typical electron-donating(D)and electron-accepting(A)structures(abbreviated as LDs-BDM,LDs-BTM,and LDs-BHM)was synthesized through a simple single-step reaction.They were found to be efficient solid-state emitters with high fluorescence quantum yields of above 70%(e.g.,83.7%for LDs-BTM).Their light-emission properties could be tuned by the modulation ofπ-conjugation effect with methoxy groups at different substituent positions.Their resulting fluorescent nanoparticles(NPs)were demonstrated as specific lipid droplets(LDs)targeting probes with high brightness,good biocompatibility,and satisfactory photostability.LDs-BTM NPs with a large two-photon absorption cross section(σ2=249 GM)were further utilized as ultrabright two-photon fluorescence(2PF)nanoprobes for in vivo retina imaging of live zebrafish by NIR excitation at an ultralow concentration(0.5μmol/L).Integrated histological structures at the tissue level and corresponding fine details at the cellular level of the embryonic retina of live zebrafish were clearly demonstrated.This is the first report of using ultrabright LDs-targeting nanoprobes to accurately measure fine details in the retina with 2PF microscopic technique.These good results are anticipated to open up a new avenue in the development of efficient 2PF emitters for non-invasive bioimaging of living animals. | Huifang Su Tao Xie Yong U.Liu Yuhan Cui Wei Wen Ben Zhong Tang Wei Qin | 2023 | Chinese Chemical Letters2023，34，6： | 0 |
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