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| 1 | Subchondral bone microenvironment in osteoarthritis and pain显示文摘Osteoarthritis comprises several joint disorders characterized by articular cartilage degeneration and persistent pain,causing disability and economic burden.The incidence of osteoarthritis is rapidly increasing worldwide due to aging and obesity trends.Basic and clinical research on osteoarthritis has been carried out for decades,but many questions remain unanswered.The exact role of subchondral bone during the initiation and progression osteoarthritis remains unclear.Accumulating evidence shows that subchondral bone lesions,including bone marrow edema and angiogenesis,develop earlier than cartilage degeneration.Clinical interventions targeting subchondral bone have shown therapeutic potential,while others targeting cartilage have yielded disappointing results.Abnormal subchondral bone remodeling,angiogenesis and sensory nerve innervation contribute directly or indirectly to cartilage destruction and pain.This review is about bone-cartilage crosstalk,the subchondral microenvironment and the critical role of both in osteoarthritis progression.It also provides an update on the pathogenesis of and interventions for osteoarthritis and future research targeting subchondral bone. | Yan Hu Xiao Chen Sicheng Wang Yingying Jing Jiacan Su | 2021 | Bone Research2021，9，3： | 24 |
| 2 | Diagnosis of brain death: confirmatory tests after clinical test显示文摘 | Su Yingying Yang Qinglin Liu Gang Zhang Yan Ye Hong Gao Daiquan Zhang Yunzhou Chen Weibi | 2014 | Chinese Medical Journal2014，，7： | 16 |
| 3 | Exosome-guided bone targeted delivery of Antagomir-188 as an anabolic therapy for bone loss显示文摘The differentiation shift from osteogenesis to adipogenesis of bone marrow mesenchymal stem cells(BMSCs)characterizes many pathological bone loss conditions.Stromal cell-derived factor-1(SDF1)is highly enriched in the bone marrow for C-X-C motif chemokine receptor 4(CXCR4)-positive hematopoietic stem cell(HSC)homing and tumor bone metastasis.In this study,we displayed CXCR4 on the surface of exosomes derived from genetically engineered NIH-3T3 cells.CXCR4+exosomes selectively accumulated in the bone marrow.Then,we fused CXCR4+exosomes with liposomes carrying antagomir-188 to produce hybrid nanoparticles(NPs).The hybrid NPs specifically gathered in the bone marrow and released antagomir-188,which promoted osteogenesis and inhibited adipogenesis of BMSCs and thereby reversed age-related trabecular bone loss and decreased cortical bone porosity in mice.Taken together,this study presents a novel way to obtain bone-targeted exosomes via surface display of CXCR4 and a promising anabolic therapeutic approach for age-related bone loss. | Yan Hu Xiaoqun Li Qin Zhang Zhengrong Gu Ying Luo Jiawei Guo Xiuhui Wang Yingying Jing Xiao Chen Jiacan Su | 2021 | Bioactive Materials2021，6，9： | 12 |
| 4 | A Comparison Study of Working Memory Deficits between Patients with Methamphetamine-Associated Psychosis and Patients with Schizophrenia显示文摘 | Hong GAN Zhenhua SONG Peiwei XU Hang SU Yingying PAN Min ZHAO Dengtang LIU | 2018 | 上海精神医学2018，30，3： | 6 |
| 5 | microRNA-21a-5p/PDCD4 axis regulates mesenchymal stem cell-induced neuroprotection in acute glaucoma显示文摘间充质的干细胞(MSC ) 被表明了为许多神经病学的疾病有有希望的治疗学的好处;然而,内在的机制糟糕被理解。这里,我们显示出 MSC 的那 intravitreal 注入在尖锐绿内障的一个老鼠模型的支持的网膜的中心细胞(RGC ) 幸存与 microglial 激活的重要抑制, TNF- , IL-1,和反应的氧种类的生产,以及 caspase-8 和 caspase-3 激活。在 vitro, MSC 禁止了两 caspase-8-mediated RGC apoptosis 和 microglial 激活,部分经由 stanniocalcin 的行动 1 (STC1 ) 。而且,我们发现了那 microRNA-21a-5p (miR-21 ) 和它的目标, PDCD4,为 STC1 生产和在 vitro 并且在 vivo 的 MSC 的 neuroprotective 性质是必要的。重要地, miR-21 overexpression 或 PDCD4 尖锐绿内障上的击倒的扩充调停 MSC 的 neuroprotective 效果。miR-21/PDCD4 轴由导致 MSC 分泌施加 neuroprotective 的 STC1 和另外的因素的以前未被认出的 neuroprotective 机制完成的这些数据热点。因此, modulating miR-21/PDCD4 轴可能是为尖锐绿内障和另外的神经病学的疾病的临床的处理的有希望的策略。 | Wenru Su Zuohong Li Yu Jia Yingting Zhu Wenjia Cai Peixing Wan Yingying Zhang Song Guo Zheng Yehong Zhuo | 2017 | Journal of Molecular Cell Biology2017，9，4： | 5 |
| 6 | Silencing of developmental genes by H3K27me3 and DNA methylation reflects the discrepant plasticity of embryonic and extraembryonic lineages显示文摘 | Xianfa Yang Boqiang Hu Yu Hou Yunbo Qiao Ran Wang Yingying Chen Yun Qian Su Feng Jun Chen Chang Liu Guangdun Peng Fuchou Tang Naihe Jing | 2018 | Cell Research2018，28，5： | 5 |
| 7 | Fabrication of physical and chemical crosslinked hydrogels for bone tissue engineering显示文摘Bone tissue engineering has emerged as a significant research area that provides promising novel tools for the preparation of biomimetic hydrogels applied in bone-related diseases(e.g.,bone defects,cartilage damage,osteoarthritis,etc.).Herein,thermal sensitive polymers(e.g.,PNIPAAm,Soluplus,etc.)were introduced into main chains to fabricate biomimetic hydrogels with injectability and compatibility for those bone defect need minimally invasive surgery.Mineral ions(e.g.,calcium,copper,zinc,and magnesium),as an indispensable role in maintaining the balance of the organism,were linked with polymer chains to form functional hydrogels for accelerating bone regeneration.In the chemically triggered hydrogel section,advanced hydrogels crosslinked by different molecular agents(e.g.,genipin,dopamine,caffeic acid,and tannic acid)possess many advantages,including extensive selectivity,rapid gel-forming capacity and tunable mechanical property.Additionally,photo crosslinking hydrogel with rapid response and mild condition can be triggered by different photoinitiators(e.g.,I2959,LAP,eosin Y,riboflavin,etc.)under specific wavelength of light.Moreover,enzyme triggered hydrogels were also utilized in the tissue regeneration due to its rapid gel-forming capacity and excellent biocompatibility.Particularly,some key factors that can determine the therapy effect for bone tissue engineering were also mentioned.Finally,brief summaries and remaining issues on how to properly design clinical-oriented hydrogels were provided in this review. | Xu Xue Yan Hu Sicheng Wang Xiao Chen Yingying Jiang Jiacan Su | 2022 | Bioactive Materials2022，7，6： | 5 |
| 8 | Weaving 3D highly conductive hierarchically interconnected nanoporous web by threading MOF crystals onto multi walled carbon nanotubes for high performance Li-Se battery显示文摘Lithium-selenium(Li-Se)battery has attracted growing attention.Nevertheless,its practical application is still impeded by the shuttle effect of the formed polyselenides.Herein,we report in-situ hydrothermal weaving the three-dimensional(3 D)highly conductive hierarchically interconnected nanoporous web by threading microporous metal organic framework MIL-68(Al)crystals onto multi-walled carbon nanotubes(MWCNTs).Such 3 D hierarchically nanoporous web(3 D MIL-68(Al)@MWCNTs web)with a very high surface area,a large amount of micropores,electrical conductivity and elasticity strongly traps the soluble polyselenides during the electrochemical reaction and significantly facilitates lithium ion diffusion and electron transportation.Molecular dynamic calculation confirmed the strong affinity of MIL-68(Al)for the adsorption of polyselenides,quite suitable for Li-Se battery.Their hexahedral channels(1.56 nm)are more efficient for the confinement of polyselenides and for the diffusion of electrolytes compared to their smaller triangular channels(0.63 nm).All these excellent characteristics of 3 D MIL-68(Al)@MWCNTs web with suitable confinement of a large amount of selenium and the conductive linkage between MIL-68(Al)host by MWCNTs result in a high capacity of 453 m Ah/g at 0.2 C with 99.5%coulombic efficiency after 200 cycles with significantly improved cycle stability and rate performance.The 3 D MIL-68(Al)@MWCNTs web presents a good performance in Li-Se battery in term of the specific capacity and cycling stability and also in terms of rate performance compared with all the metal-organic framework(MOF)based or MOF derived porous carbons used in Li-Se battery. | Chao Li Yingying Wang Hongyan Li Jing Liu Jianping Song Luca Fusaro Zhi-Yi Hu Yanxin Chen Yu Li Bao-Lian Su | 2021 | Journal of Energy Chemistry2021，30，8： | 4 |
| 9 | Bone/cartilage organoid on-chip:Construction strategy and application显示文摘The necessity of disease models for bone/cartilage related disorders is well-recognized,but the barrier between ex-vivo cell culture,animal models and the real human body has been pending for decades.The organoid-on-a-chip technique showed opportunity to revolutionize basic research and drug screening for diseases like osteoporosis and arthritis.The bone/cartilage organoid on-chip(BCoC)system is a novel platform of multi-tissue which faithfully emulate the essential elements,biologic functions and pathophysiological response under real circumstances.In this review,we propose the concept of BCoC platform,summarize the basic modules and current efforts to orchestrate them on a single microfluidic system.Current disease models,unsolved problems and future challenging are also discussed,the aim should be a deeper understanding of diseases,and ultimate realization of generic ex-vivo tools for further therapeutic strategies of pathological conditions. | Yan Hu Hao Zhang Sicheng Wang Liehu Cao Fengjin Zhou Yingying Jing Jiacan Su | 2023 | Bioactive Materials2023，，7： | 3 |
| 10 | Detection of Porcine Epidemic Diarrhea Virus in Guangxi Province from 2011 to 2014 and Sequence Analysis of Its M Gene显示文摘Detection of pigs epidemic diarrhea virus( PEDV) was conducted on 331 piglets diarrhea fecal samples collected in Nanning,Yulin and other 12 areas of Guangxi Province from January of 2011 to April of 2014 by the method of reverse transcription-polymerase chain reaction( RT-PCR). The results showed that the positive samples of PEDV were 210 and the positive rate was 63. 44%. The clone and sequencing of M gene was carried out on 25 positive samples. PEDV reference strains were selected from Gene Bank to conduct the sequence homology alignment analysis and the phylogenetic tree of M gene. The M gene homology and amino acid sequence identity between 25 isolated strains and 51 reference strains were 96. 0%- 99. 6% and 94. 3%- 99. 6%,respectively. The genetic variation analysis of M gene showed that the genetic relationship of PEDV prevalent strains in Guangxi Province from 2013 to 2014 was close to that of the prevalent strains in Beijing,Anhui,Wuhan,Hebei and Guangdong from 2010 to 2013,and which were far from that of the Chinese early isolates CH / S( Gen Bank number: JN547228),vaccine strain CV777( Gen Bank number: AF353511) and Attenuated DR13( Gen Bank number: JQ023162). Indicating that the PEDV strains prevalent in Guangxi in recent years showed significant variation with the early isolates. | Lu Bingxia Qin Yibin He Ying Li Yingying Liang Jiaxing Li Keyu Li Bin Su Qianlian Zhou Yingning Jiang Dongfu Lu Jingzhuan Bi Bingfen Liang Baozhong Duan Qunpeng Zhao Wu | 2016 | Animal Husbandry and Feed Science2016，8，1： | 3 |
| 11 | Targeting slug-mediated non-canonical activation of c-Met to overcome chemo-resistance in metastatic ovarian cancer cells显示文摘Metastasis-associated drug resistance accounts for high mortality in ovarian cancer and remains to be a major barrier for effective treatment. In this study, SKOV3/T4, a metastatic subpopulation of ovarian cancer SKOV3 cells, was enriched to explore potential interventions against metastaticassociated drug resistance. Quantitative genomic and functional analyses were performed and found that slug was significantly increased in the SKOV3/T4 subpopulation and contributed to the high resistance of SKOV3/T4. Further studies showed that slug activated c-Met in a ligand-independent manner due to elevated levels of fibronectin and provoked integrin α V function, which was confirmed by the significant correlation of slug and p-Met levels in 121 ovarian cancer patient samples. Intriguingly,c-Met inhibitor(s) exhibited greatly enhanced anti-cancer effects in slug-positive ovarian cancer models both in vitro and in vivo. Additionally, IHC analyses revealed that slug levels were highly correlated with reduced survival of ovarian cancer patients. Taken together, this study not only uncovers the critical roles of slug in drug resistance in ovarian cancer but also highlights a promising therapeutic strategy by targeting the noncanonical activation of c-Met in slug-positive ovarian cancer patients with poor prognosis. | Linlin Chang Yan Hu Yingying Fu Tianyi Zhou Jun You Jiamin Du Lin Zheng Ji Cao Meidan Ying Xiaoyang Dai Dan Su Qiaojun He Hong Zhu Bo Yang | 2019 | Acta Pharmaceutica Sinica B2019，9，3： | 3 |
| 12 | The prior rules of designing Ti_(3)C_(2)T_(x) MXene-based gas sensors显示文摘Working temperature,sensitivity,and selectivity are some of the characteristics of the applied gas sensors.How to design and fabricate an ideal gas sensor working at room temperature is still challenging and attracting lots of interest.Two-dimensional(2D)materials with ultra-thin structure have been demonstrated as a family of ideal candidates to achieve this goal.Among them,Ti_(3)C_(2)T_(x) MXene,a kind of layered sheet synthesized by selectively etching MAX phases materials,shows remarkable potential to be the sensitive materials solely or in a composite.However,their designing rules are still lacking critical thinking from the viewpoint of the intrinsic property of Ti_(3)C_(2)T_(x) MXene based materials.In this article,two critical features,i.e.,the thickness of the sensitive materials,and the scope of the analytes,are elaborated towards Ti_(3)C_(2)T_(x) MXene based gas sensors after characterizing the performance of sensing reducing gases(NH3 and CO)and oxidizing gas(NO2).First,the thinner the Ti_(3)C_(2)T_(x) MXene sensitive layer,the better the sensitivity.Second,the Ti_(3)C_(2)T_(x) MXene based gas sensor is not suitable for strong and moderate oxidation gas due to its ease of oxidation.These two rules are demonstrated,and could be considered with priority both in the future researches and practical applications. | Yingying Jian Danyao Qu Lihao Guo Yujin Zhu Chen Su Huanran Feng Guangjian Zhang Jia Zhang Weiwei Wu Ming-Shui Yao | 2021 | Frontiers of Chemical Science and Engineering2021，15，3： | 3 |
| 13 | Selection and identification of a novel ssDNA aptamer targeting human skeletal muscle显示文摘Skeletal muscle disorders have posed great threats to health.Selective delivery of drugs and oligonucleotides to skeletal muscle is challenging.Aptamers can improve targeting efficacy.In this study,for the first time,the human skeletal muscle-specific ssDNA aptamers(HSM01,etc.)were selected and identified with Systematic Evolution of Ligands by Exponential Enrichment(SELEX).The HSM01 ssDNA aptamer preferentially interacted with human skeletal muscle cells in vitro.The in vivo study using tree shrews showed that the HSM01 ssDNA aptamer specifically targeted human skeletal muscle cells.Furthermore,the ability of HSM01 ssDNA aptamer to target skeletal muscle cells was not affected by the formation of a disulfide bond with nanoliposomes in vitro or in vivo,suggesting a potential new approach for targeted drug delivery to skeletal muscles via liposomes.Therefore,this newly identified ssDNA aptamer and nanoliposome modification could be used for the treatment of human skeletal muscle diseases. | Shuming Sun Han Liu Yan Hu Yanpeng Wang Mingri Zhao Yijun Yuan Yafei Han Yingying Jing Jin Cui Xiaoxiang Ren Xiao Chen Jiacan Su | 2023 | Bioactive Materials2023，，2： | 3 |
| 14 | Quercetin promotes human epidermal stem cell proliferation through the estrogen receptor/β-catenin/c-Myc/cyclin A2 signaling pathway显示文摘Skin epidermal stem cells(EpSCs)play an important role in wound healing.Quercetin is a phytoestrogen reported to accelerate skin wound healing,but its effect on EpSCs is unknown.In this study,we investigated the effect of quercetin on human EpSC proliferation and explored the underlying mechanisms.We found that quercetin at 0.1~1μM significantly promoted EpSC proliferation and increased the number of cells in S phase.The pro-proliferative effect of quercetin on EpSCs was confirmed in cultured human skin tissue.Mechanistic studies showed that quercetin significantly upregulated the expressions ofβ-catenin,c-Myc,and cyclins A2 and E1.Inhibitor forβ-catenin or c-Myc significantly inhibited quercetin-induced EpSC proliferation.Theβ-catenin inhibitor XAV-939 suppressed quercetin-induced expressions ofβ-catenin,c-Myc,and cyclins A2 and E1.The c-Myc inhibitor 10058-F4 inhibited the upregulation of c-Myc and cyclin A2 by quercetin.Pretreatment of EpSCs with estrogen receptor(ER)antagonist ICI182780,but not the G protein-coupled ER1 antagonist G15,reversed quercetin-induced cell proliferation and upregulation ofβ-catenin,c-Myc,and cyclin A2.Collectively,these results indicate that quercetin promotes EpSC proliferation through ER-mediated activation ofβ-catenin/c-Myc/cyclinA2 signaling pathway and ER-independent upregulation of cyclin E1 and that quercetin may accelerate skin wound healing through promoting EpSC proliferation.As EpSCs are used not only in clinic to treat skin wounds but also as seed cells in skin tissue engineering,quercetin is a useful reagent to expand EpSCs for basic research,skin wound treatment,and skin tissue engineering. | Zhaodong Wang Guangliang Zhang Yingying Le Jihui Ju Ping Zhang Dapeng Wan Qiang Zhao Guangzhe Jin Hao Su Jinwei Liu Jiaxuan Feng Yi Fu Ruixing Hou | 2020 | Acta Biochimica et Biophysica Sinica2020，52，10： | 3 |
| 15 | GFAP-Positive Progenitor Cell Production is Concentrated in Specific Encephalic Regions in Young Adult Mice显示文摘Previous genetic fate-mapping studies have indicated that embryonic glial fibrillary acidic proteinpositive(GFAP^+) cells are multifunctional progenitor/neural stem cells that can produce astrocytes as well as neurons and oligodendrocytes throughout the adult mouse central nervous system(CNS). However, emerging evidence from recent studies indicates that GFAP^+cells adopt different cell fates and generate different cell types in different regions. Moreover, the fate of GFAP^+cells in the young adult mouse CNS is not well understood. In the present study, hGFAP-Cre/R26 R transgenic mice were used to investigate the lineage of embryonic GFAP^+cells in the young adult mouse CNS. At postnatal day 21, we found that GFAP^+cells mainly generated NeuN^+neurons in the cerebral cortex(both ventral and dorsal), hippocampus, and cerebellum. Strangely, these cells were negative for the Purkinje cell marker calbindin in the cerebellum and the neuronal marker NeuN in the thalamus. Thus,contrary to previous studies, our genetic fate-mapping revealed that the cell fate of embryonic GFAP^+cells at the young adult stage is significantly different from that at the adult stage. | Zhibao Guo Yingying Su Huifang Lou | 2018 | Neuroscience Bulletin2018，34，5： | 3 |
| 16 | Multi-omic characterization of genome-wide abnormal DNA methylation reveals diagnostic and prognostic markers for esophageal squamous-cell carcinoma显示文摘This study investigates aberrant DNA methylations as potential diagnosis and prognosis markers for esophageal squamous-cell carcinoma(ESCC),which if diagnosed at advanced stages has<30%five-year survival rate.Comparing genome-wide methylation sites of 91 ESCC and matched adjacent normal tissues,we identified 35,577 differentially methylated CpG sites(DMCs)and characterized their distribution patterns. | Yiyi Xi Yuan Lin Wenjia Guo Xinyu Wang Hengqiang Zhao Chuanwang Miao Weiling Liu Yachen Liu Tianyuan Liu Yingying Luo Wenyi Fan Ai Lin Yamei Chen Yanxia Sun Yulin Ma Xiangjie Niu Ce Zhong Wen Tan Meng Zhou Jianzhong Su Chen Wu Dongxin Lin | 2022 | Signal Transduction and Targeted Therapy2022，7，3： | 2 |
| 17 | An Organic Solvent-Assisted Intercalation and Collection (OAIC) for Ti_(3)C_(2)T_(x) MXene with Controllable Sizes and Improved Yield显示文摘A good method of synthesizing Ti_(3)C_(2)T_(x)(MXene)is critical for ensuring its success in practical applications,e.g.,electromagnetic interference shielding,electrochemical energy storage,catalysis,sensors,and biomedicine.The main concerns focus on the moderation of the approach,yield,and product quality.Herein,a modified approach,organic solvent-assisted intercalation and collection,was developed to prepare Ti_(3)C_(2)T_(x) flakes.The new approach simultaneously solves all the concerns,featuring a low requirement for facility(centrifugation speed<4000 rpm in whole process),gram-level preparation with remarkable yield(46.3%),a good electrical conductivity(8672 S cm^(−1)),an outstanding capacitive performance(352 F g^(−1)),and easy control over the dimension of Ti_(3)C_(2)T_(x) flakes(0.47–4.60μm^(2)).This approach not only gives a superb example for the synthesis of other MXene materials in laboratory,but sheds new light for the future mass production of Ti_(3)C_(2)T_(x) MXene. | Danyao Qu Yingying Jian Lihao Guo Chen Su Ning Tang Xingmao Zhang Wenwen Hu Zheng Wang Zhenhuan Zhao Peng Zhong Peipei Li Tao Du Hossam Haick Weiwei Wu | 2021 | Nano-Micro Letters2021，13，11： | 2 |
| 18 | Insulin sensitivity,βcell function,and adverse pregnancy outcomes in women with gestational diabetes显示文摘Background: The potential impact of β cell function and insulin sensitivity on adverse pregnancy outcomes in women with gestational diabetes mellitus (GDM) remains uncertain. We aimed to investigate the association between β cell dysfunction, insulin resistance, and the composite adverse pregnancy outcomes.Methods: This observational study included 482 women diagnosed with GDM during pregnancy. Quantitative metrics on β cell function and insulin sensitivity during pregnancy were calculated using traditional equations. The association of β cell dysfunction and insulin resistance with the risk of the composite adverse pregnancy outcomes was investigated using multivariable-adjusted logistic regression models.Results: Multivariable-adjusted odds ratios (ORs) of adverse pregnancy outcomes across quartiles of homeostatic model assessment for insulin resistance (HOMA-IR) were 1.00, 0.95, 1.34, and 2.25, respectively (P for trend = 0.011). When HOMA-IR was considered as a continuous variable, the multivariable-adjusted OR of adverse pregnancy outcomes was 1.34 (95% confidence interval 1.16-1.56) for each 1-unit increase in HOMA-IR. Multivariable-adjusted ORs of adverse pregnancy outcomes across quartiles of homeostatic model assessment for β cell function (HOMA-β) were 1.00, 0.51, 0.60, and 0.53, respectively (P for trend = 0.068). When HOMA-β was considered as a continuous variable, the multivariable-adjusted OR of adverse pregnancy outcomes was 0.57 (95% CI 0.24-0.90) for each 1-unit increase in HOMA-β. However, other quantitative metrics were not associated with the composite adverse pregnancy outcomes.Conclusions: We demonstrated a significant association of β cell function and insulin sensitivity with the risk of adverse pregnancy outcomes. We have provided additional evidence on the early identification of adverse pregnancy outcomes besides the glycemic values. | Yun Shen Yanwei Zheng Yingying Su Susu Jiang Xiaojing Ma Jiangshan Hu Changbin Li Yajuan Huang Yincheng Teng Yuqian Bao Minfang Tao Jian Zhou | 2022 | Chinese Medical Journal2022，135，21： | 2 |
| 19 | Overexpression of Sirt6 ameliorates sleep deprivation induced-cognitive impairment by modulating glutamatergic neuron function显示文摘Sleep benefits the restoration of energy metabolism and thereby suppo rts neuronal plasticity and cognitive behaviors.Sirt6 is a NAD+-dependent protein deacetylase that has been recognized as an essential regulator of energy metabolism because it modulates various transcriptional regulators and metabolic enzymes.The aim of this study was to investigate the influence of Sirt6 on cerebral function after chronic sleep deprivation(CSD).We assigned C57BL/6J mice to control or two CSD groups and subjected them to AAV2/9-CMV-EGFP or AAV2/9-CMV-Sirt6-EGFP infection in the prelimbic cortex(PrL).We then assessed cerebral functional connectivity(FC) using resting-state functional MRI,neuron/astrocyte metabolism using a metabolic kinetics analysis;dendritic spine densities using sparse-labeling;and miniature excitato ry postsynaptic currents(mEPSCs) and action potential(AP) firing rates using whole-cell patchclamp recordings.In addition,we evaluated cognition via a comprehensive set of behavioral tests.Compared with controls,Sirt6 was significantly decreased(P<0.05) in the PrL after CSD,accompanied by cognitive deficits and decreased FC between the PrL and accumbens nucleus,piriform cortex,motor co rtex,somatosensory co rtex,olfactory tubercle,insular cortex,and cerebellum.Sirt6 ove rexpression reve rsed CSD-induced cognitive impairment and reduced FC.Our analysis of metabolic kinetics using [1-13C] glucose and [2-13C] acetate showed that CSD reduced neuronal Glu4and GABA2synthesis,which could be fully restored via forced Sirt6 expression.Furthermore,Sirt6 ove rexpression reversed CSD-induced decreases in AP firing rates as well as the frequency and amplitude of mEPSCs in PrL pyramidal neurons.These data indicate that Sirt6 can improve cognitive impairment after CSD by regulating the PrL-associated FC network,neuronal glucose metabolism,and glutamatergic neurotransmission.Thus,Sirt6 activation may have potential as a novel strategy for treating sleep disorder-related diseases. | Jinpiao Zhu Chang Chen Zhen Li Xiaodong Liu Jingang He Ziyue Zhao Mengying He Binbin Nie Zili Liu Yingying Chen Kuanpin Su Xiang Li Juxiang Chen Hongbing Xiang Fuqiang Xu Kangguang Lin Zongze Zhang Jie Wang | 2023 | Neural Regeneration Research2023，18，11： | 1 |
| 20 | Rapid, sensitive and on-line measurement of chemical oxygen demand by novel optical method based on UV photolysis and chemiluminescence显示文摘 | Yingying Su Xiaohong Li He Chen Yi Lv Xia- odeng Hou | 2007 | Microchemical Journal2007，，87： | 1 |
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