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| 1 | Three-dimensional-printed individualized porous implants:A new“implant-bone”interface fusion concept for large bone defect treatment显示文摘Bone defect repairs are based on bone graft fusion or replacement.Current large bone defect treatments are inadequate and lack of reliable technology.Therefore,we aimed to investigate a simple technique using three-dimensional(3D)-printed individualized porous implants without any bone grafts,osteoinductive agents,or surface biofunctionalization to treat large bone defects,and systematically study its long-term therapeutic effects and osseointegration characteristics.Twenty-six patients with large bone defects caused by tumor,infection,or trauma received treatment with individualized porous implants;among them,three typical cases underwent a detailed study.Additionally,a large segmental femur defect sheep model was used to study the osseointegration characteristics.Immediate and long-term biomechanical stability was achieved,and the animal study revealed that the bone grew into the pores with gradual remodeling,resulting in a long-term mechanically stable implant-bone complex.Advantages of 3D-printed microporous implants for the repair of bone defects included 1)that the stabilization devices were immediately designed and constructed to achieve early postoperative mobility,and 2)that osseointegration between the host bone and implants was achieved without bone grafting.Our osseointegration method,in which the“implant-bone”interface fusion concept was used instead of“bone-bone”fusion,subverts the traditional idea of osseointegration. | Teng Zhang Qingguang Wei Hua Zhou Zehao Jing Xiaoguang Liu Yufeng Zheng Hong Cai Feng Wei Liang Jiang Miao Yu Yan Cheng Daoyang Fan Wenhao Zhou Xinhong Lin Huijie Leng Jian Li Xinyu Li Caimei Wang Yun Tian Zhongjun Liu | 2021 | Bioactive Materials2021，6，11： | 9 |
| 2 | Hierarchical Micropore/Nanorod Apatite Hybrids In-Situ Grown from 3-D Printed Macroporous Ti6Al4V Implants with Improved Bioactivity and Osseointegration显示文摘The advent of three-dimensional(3-D) printed technique provides great possibility in the fabrication of customized porous titanium(Ti) implant. However, the bioinert property of the printed Ti poses an outstanding problem. Hybrid micro-arc oxidation and hydrothermal(MAO–HT) treatment on porous metals is able to produce multi-scaled hierarchical orthopedic implant, showing great potential for surface modification of 3-D printed implant. In this study, cylindrical porous Ti6Al4V(Ti64) scaffolds with pore size of 640 μm, porosity of 73% were 3-D printed by electron beam melting process, and their surfaces were left untreated or treated by a combined MAO–HT procedure. In vitro bioactivity was tested by immersion in simulated body fluid for different time points. Then, 12 scaffolds in each group were implanted into the femoral condyles of New Zealand rabbit for 8 weeks. Osseointegration was evaluated by qualitative and quantitative histological analysis, and the bone ingrowth features were probed by sequential fluorescent labeling at 3 and 6 weeks post-surgery. Following the MAO–HT treatment, the porous Ti64 scaffold was endowed with multi-scaled micro/nano-topographies and high amounts of Ca P on its surface.The treated scaffold exhibited drastically enhanced apatite forming ability compared with the untreated one. In vivo test revealed significantly that a higher amount of bone ingrowth and bone implant contact at the treated scaffold. The 2 types of scaffolds had different patterns of bone ingrowth: the treated scaffold exhibited a pattern of contact osteogenesis, by which bone formed directly on the treated implant surface, whereas bone formed distal to the implant surface of the untreated scaffold. MAO–HT treatment can significantly enhance the in vitro apatite-inducing ability and in vivo osseointegration capacity of 3-D porous Ti64 scaffold and may provide as a viable approach for the fabrication of bioactive 3-D printed porous implant for orthopedic applications. | Peng Xiu Zhaojun Jia Jia Lv Chuan Yin Hong Cai Chunli Song Huijie Leng Yufeng Zheng Zhongjun Liu Yan Cheng | 2017 | Journal of Materials Science & Technology2017，33，2： | 2 |
| 3 | Safety and efficacy of meplazumab in healthy volunteers and COVID-19 patients: a randomized phase 1 and an exploratory phase 2 trial显示文摘Recent evidence suggests that CD147 serves as a novel receptor for severe acute respiratory syndrome coronavirus 2(SARS-CoV-2)infection.Blocking CD147 via anti-CD147 antibody could suppress the in vitro SARS-CoV-2 replication.Meplazumab is a humanized anti-CD147 IgG_(2) monoclonal antibody,which may effectively prevent SARS-CoV-2 infection in coronavirus disease 2019(COVID-19)patients.Here,we conducted a randomized,double-blinded,placebo-controlled phase 1 trial to evaluate the safety,tolerability,and pharmacokinetics of meplazumab in healthy subjects,and an open-labeled,concurrent controlled add-on exploratory phase 2 study to determine the efficacy in COVID-19 patients.In phase 1 study,59 subjects were enrolled and assigned to eight cohorts,and no serious treatment-emergent adverse event(TEAE)or TEAE grade≥3 was observed.The serum and peripheral blood Cmax and area under the curve showed non-linear pharmacokinetic characteristics.No obvious relation between the incidence or titer of positive anti-drug antibody and dosage was observed in each cohort.The biodistribution study indicated that meplazumab reached lung tissue and maintained>14 days stable with the lung tissue/cardiac blood-pool ratio ranging from 0.41 to 0.32.In the exploratory phase 2 study,17 COVID-19 patients were enrolled,and 11 hospitalized patients were involved as concurrent control.The meplazumab treatment significantly improved the discharged(P=0.005)and case severity(P=0.021),and reduced the time to virus negative(P=0.045)in comparison to the control group.These results show a sound safety and tolerance of meplazumab in healthy volunteers and suggest that meplazumab could accelerate the recovery of patients from COVID-19 pneumonia with a favorable safety profile. | Huijie Bian Zhao-Hui Zheng Ding Wei Aidong Wen Zheng Zhang Jian-Qi Lian Wen-Zhen Kang Chun-Qiu Hao Jing Wang Rong-Hua Xie Ke Dong Jie-Lai Xia Jin-Lin Miao Wen Kang Guoquan Li Di Zhang Mingru Zhang Xiu-Xuan Sun Likun Ding Kui Zhang Junfeng Jia Jin Ding Zhiqin Li Yanyan Jia Lin-Na Liu Zhe Zhang Zhao-Wei Gao Hong Du Na Yao Qing Wang Ke Wang Jie-Jie Geng Bin Wang Ting Guo Ruo Chen Yu-Meng Zhu Li-Juan Wang Qian He Rui-Rui Yao Ying Shi Xiang-Min Yang Jian-Sheng Zhou Yi-Nan Ma Ya-Tao Wang Xue Liang Fei Huo Zhe Wang Yang Zhang Xu Yang Ye Zhang Lu-Hua Gao Ling Wang Xiao-Chun Chen Hao Tang Shuang-Shuang Liu Qing-Yi Wang Zhi-Nan Chen Ping Zhu | 2021 | Signal Transduction and Targeted Therapy2021，6，6： | 2 |
| 4 | Surface treatment of 3D printed porous Ti6Al4V implants by ultraviolet photofunctionalization for improved osseointegration显示文摘Three-dimensional(3D)-printed porous Ti6Al4V implants play an important role in the reconstruction of bone defects.However,its osseointegration capacity needs to be further improved,and related methods are inadequate,especially lacking customized surface treatment technology.Consequently,we aimed to design an omnidirectional radiator based on ultraviolet(UV)photofunctionalization for the surface treatment of 3D-printed porous Ti6Al4V implants,and studied its osseointegration promotion effects in vitro and in vivo,while elucidating related mechanisms.Following UV treatment,the porous Ti6Al4V scaffolds exhibited significantly improved hydrophilicity,cytocompatibility,and alkaline phosphatase activity,while preserving their original mechanical properties.The increased osteointegration strength was further proven using a rabbit condyle defect model in vivo,in which UV treatment exhibited a high efficiency in the osteointegration enhancement of porous Ti6Al4V scaffolds by increasing bone ingrowth(BI),the bone-implant contact ratio(BICR),and the mineralized/osteoid bone ratio.The advantages of UV treatment for 3D-printed porous Ti6Al4V implants using the omnidirectional radiator in the study were as follows:1)it can significantly improve the osseointegration capacity of porous titanium implants despite the blocking out of UV rays by the porous structure;2)it can evenly treat the surface of porous implants while preserving their original topography or other morphological features;and 3)it is an easy-to-operate low-cost process,making it worthy of wide clinical application. | Chuan Yin Teng Zhang Qingguang Wei Hong Cai Yan Cheng Yun Tian Huijie Leng Caimei Wang Shiqing Feng Zhongjun Liu | 2022 | Bioactive Materials2022，7，1： | 2 |
| 5 | Amine modified graphene as reversed-dispersive solid phase extraction materials combined with liquid chromatography-tandem mass spectrometry for pesticide multi-residue analysis in oil crops显示文摘 | Wenbi Guan Zhuonan Li Hongyan Zhang Huijie Hong Natalie Rebeyev Yong Ye Yongqiang Ma | 2013 | Journal of Chromatography A2013，，： | 1 |
| 6 | Analysis of Genetic Relationship among Celery Germplasms by RAPD显示文摘In this study,forty celery cultivars introduced from different regions of China were used as experimental materials to verify and analyze the practicability of RAPD technology in the identification of celery cultivars. The results showed that RAPD technology could distinguish accurately the genetic relationship among various celery cultivars as a simple and ideal DNA molecular marker technology suitable for genetic relationship analysis. To be specific,13 RAPD primers with clear amplified bands and significantly different number of amplified bands were screened,which provided basis for the identification of genetic relationship among different celery cultivars. | Pangyuan LIU Baohai ZHANG Hong ZHAO Weiming HE Huijie WANG | 2014 | Agricultural Biotechnology2014，3，2： | 1 |
| 7 | Plant leaves recognition and classification model based on image features and neural network显示文摘 | Fang Hong Li Huijie | 2014 | International Journal of Computer Science Issues2014，11，2： | 1 |
| 8 | Determination and Study on Residue and Dissipation of Florasulam in Wheat and SoilUnder Field Conditions显示文摘 | Zhuonan Li Wenbi Guan Huijie Hong | 2013 | Bull Environ Contam Toxicol2013，90，： | 1 |
| 9 | Rational Design of Thermosensitive Hydrogel to Deliver Nanocrystals with Intranasal Administration for Brain Targeting in Parkinson’s Disease显示文摘Mitochondrial dysfunction is commonly detected in individuals suffering from Parkinson’s disease(PD),presenting within the form of excessive reactive oxygen species(ROS)generation as well as energy metabolism.Overcoming this dysfunction within brain tissues is an effective approach to treat PD,while unluckily,the blood-brain barrier(BBB)substantially impedes intracerebral drug delivery.In an effort to improve the delivery of efficacious therapeutic drugs to the brain,a drug delivery platform hydrogel(MAG-NCs@Gel)was designed by complexing magnolol(MAG)-nanocrystals(MAG-NCs)into the noninvasive thermosensitive poly(Nisopropylacrylamide)(PNIPAM)with self-gelation.The as-prepared MAG-NCs@Gel exhibited obvious improvements in drug solubility,the duration of residence with the nasal cavity,and the efficiency of brain targeting,respectively.Above all,continuous intranasal MAG-NCs@Gel delivery enabled MAG to cross the BBB and enter dopaminergic neurons,thereby effectively alleviating the symptoms of MPTP-induced PD.Taking advantage of the lower critical solution temperature(LCST)behavior of this delivery platform increases its viscoelasticity in nasal cavity,thus improving the efficiency of MAG-NCs transit across the BBB.As such,MAG-NCs@Gel represented an effective delivery platform capable of normalizing ROS and adenosine triphosphate(ATP)in the mitochondria of dopaminergic neurons,consequently reversing the mitochondrial dysfunction and enhancing the behavioral skills of PD mice without adversely affecting normal tissues. | Yun Tan Yao Liu Yujing Liu Rui Ma Jingshan Luo Huijie Hong Xiaojia Chen Shengpeng Wang Chuntai Liu Yi Zhang Tongkai Chen | 2021 | Research2021，，1： | 1 |
| 10 | Development of an up-converting phosphor technology-based 10-channel lateral flow assay for profiling antibodies against Yersinia pestis显示文摘 | Wenyan Hong Lihua Huang Haoran Wang Jianfeng Qu Zhaobiao Guo Chengke Xie Ziwen Zhu Youbao Zhang Zongmin Du Yanfeng Yan Yan Zheng Huijie Huang Ruifu Yang Lei Zhou | 2010 | Journal of Microbiological Methods2010，，2： | 1 |
| 11 | Graphene-dispersive solid-phase extraction of phthalate acid esters from environmental water显示文摘 | WU Xiaoli HONG Huijie LIU Xiaotong | 2013 | Science of The Total Environment2013，444，1： | 1 |
| 12 | Photodegradation of selected β-blockers in aqueous fulvic acid solutions: Kinetics, mechanism, and product analysis显示文摘 | Yong Chen Hong Li Zongping Wang Huijie Li Tao Tao Yuegang Zuo | 2012 | Water Research2012，，9： | 1 |
| 13 | Robust boron nanoplatform provokes potent tumoricidal activities via inhibiting heat shock protein显示文摘Near-infrared(NIR)-light-triggered photothermal therapy(PTT)is a promising treatment for breast cancer.However,its therapeutic efficiency is often compromised due to the heatinduced up-regulation of heat shock proteins,which confer photothermal resistance.To solve this urgent problem,PEGylated two-dimensional boron nanosheets(B-PEG)-which allow both multimodal imaging and photothermal conversion-were loaded with gambogic acid(GA),which can inhibit heat shock protein 90(Hsp90).Experimental findings indicated that this combination of B-PEG and GA could serve as an integrated drug delivery system for cancer diagnosis and treatment.It could be used to administer mild PTT as well as chemotherapy for breast cancer,provide improved anti-tumor effects,and reduce the toxicity of PTT,all while inhibiting breast cancer growth.This drug delivery system could offer a novel tool for administering chemotherapy combined with PTT while avoiding the adverse effects of traditional PTT. | Yuying Zhao Ning Liu Piaoxue Liu Taojian Fan Rui Ma Huijie Hong Xiaojia Chen Zhongjian Xie Han Zhang Qi Wang Tongkai Chen | 2022 | Asian Journal of Pharmaceutical Sciences2022，17，5： | 0 |
| 14 | Simvastatin-hydroxyapatite coatings prevent biofilm formation and improve bone formation in implant-associated infections显示文摘Implant-associated infections(IAIs)caused by biofilm formation are the most devastating complications of orthopedic surgery.Statins have been commonly and safely used drugs for hypercholesterolemia for many years.Here,we report that simvastatin-hydroxyapatite-coated titanium alloy prevents biofilm-associated infections.The antibacterial properties of simvastatin against Staphylococcus aureus and Staphylococcus epidermidis biofilms in vitro was confirmed by crystal violet staining and live-dead bacterial staining.We developed a simvastatin-and hydroxyapatite(Sim-HA)-coated titanium alloy via electrochemical deposition.Sim-HA coatings inhibited Staphylococcus aureus biofilm formation and improved the biocompatibility of the titanium alloy.Sim-HA coatings effectively prevented Staphylococcus aureus IAI in rat femurs,as confirmed by radiological assessment and histological examination.The antibacterial effects of the Sim-HA coatings were attributed to their inhibitory effects on biofilm formation,as verified by scanning electron microscopic observations and bacterial spread plate analysis.In addition,the Sim-HA coatings enhanced osteogenesis and osteointegration,as verified by micro-CT,histological evaluation,and biomechanical pull-out tests.In summary,Sim-HA coatings are promising implant materials for protection against biofilm-associated infections. | Tiantong Sun Jie Huang Wang Zhang Xuanqi Zheng Hong Wang Jing Liu Huijie Leng Wanqiong Yuan Chunli Song | 2023 | Bioactive Materials2023，，3： | 0 |
| 15 | Rational Design of Thermosensitive Hydrogel to Deliver Nanocrystals with Intranasal Administration for Brain Targeting in Parkinson’s Disease显示文摘Mitochondrial dysfunction is commonly detected in individuals suffering from Parkinson’s disease(PD),presenting within the form of excessive reactive oxygen species(ROS)generation as well as energy metabolism.Overcoming this dysfunction within brain tissues is an effective approach to treat PD,while unluckily,the blood-brain barrier(BBB)substantially impedes intracerebral drug delivery.In an effort to improve the delivery of efficacious therapeutic drugs to the brain,a drug delivery platform hydrogel(MAG-NCs@Gel)was designed by complexing magnolol(MAG)-nanocrystals(MAG-NCs)into the noninvasive thermosensitive poly(Nisopropylacrylamide)(PNIPAM)with self-gelation.The as-prepared MAG-NCs@Gel exhibited obvious improvements in drug solubility,the duration of residence with the nasal cavity,and the efficiency of brain targeting,respectively.Above all,continuous intranasal MAG-NCs@Gel delivery enabled MAG to cross the BBB and enter dopaminergic neurons,thereby effectively alleviating the symptoms of MPTP-induced PD.Taking advantage of the lower critical solution temperature(LCST)behavior of this delivery platform increases its viscoelasticity in nasal cavity,thus improving the efficiency of MAG-NCs transit across the BBB.As such,MAG-NCs@Gel represented an effective delivery platform capable of normalizing ROS and adenosine triphosphate(ATP)in the mitochondria of dopaminergic neurons,consequently reversing the mitochondrial dysfunction and enhancing the behavioral skills of PD mice without adversely affecting normal tissues. | Yun Tan Yao Liu Yujing Liu Rui Ma Jingshan Luo Huijie Hong Xiaojia Chen Shengpeng Wang Chuntai Liu Yi Zhang Tongkai Chen | 2022 | Research2022，，1： | 0 |
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