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| 1 | Component analysis of Chinese medicine and advances in fuming-washing therapy for knee osteoarthritis via unsupervised data mining methods显示文摘OBJECTIVE: To analyze the component law of Chinese medicines in fuming-washing therapy for knee osteoarthritis(KOA), and develop new fuming-washing prescriptions for KOA through unsupervised data mining methods.METHODS: Chinese medicine recipes for fuming-washing therapy for KOA were collected and recorded in a database. The correlation coefficient among herbs, core combinations of herbs, andnew prescriptions were analyzed using modified mutual information, complex system entropy cluster, and unsupervised hierarchical clustering, respectively.RESULTS: Based on analysis of 345 Chinese medicine recipes for fuming-washing therapy, 68 herbs occurred frequently, 33 herb pairs occurred frequently, and 12 core combinations were found.Five new fuming-washing recipes for KOA were developed.CONCLUSION: Chinese medicines for fuming-washing therapy of KOA mainly consist of wind-dampness-dispelling and cold-dispersing herbs, blood-activating and stasis-resolving herbs,and wind-dampness-dispelling and heat-clearing herbs. The treatment of fuming-washing therapy for KOA also includes dispelling wind-dampness and dispersing cold, activating blood and resolving stasis, and dispelling wind-dampness and clearing heat. Zhenzhutougucao(Herba Speranskiae Tuberculatae), Honghua(Flos Carthami), Niuxi(Radix Achyranthis Bidentatae), Shenjincao(Herba Lycopodii Japonici), Weilingxian(Radix et Rhizoma Clematidis Chinensis), Chuanwu(Radix Aconiti), Haitongpi(Cortex Erythrinae Variegatae), Ruxiang(Olibanum),Danggui(Radix Angelicae Sinensis), Caowu(Radix Aconiti Kusnezoffii), Moyao(Myrrha), and Aiye(Folium Artemisiae Argyi) are the main herbs used in the fuming-washing treatment for KOA. | Jun Liu Jianke Pan Yanping Wang Dingkun Lin Dan Shen Hongjun Yang Xiang Li Minghui Luo Xuewei Cao | 2013 | Journal of Traditional Chinese Medicine2013，33，5： | 32 |
| 2 | Spatial Structural Pattern and Vulnerability of China-Japan-Korea Shipping Network显示文摘The economies of China-Japan-Korea(CJK) are complementary, with their proximity resulting in the three countries having a high degree of interdependence with respect to trade. Currently, trade among these countries relies mainly on port-centered shipping. The development of the shipping network is integral for in-depth integration of CJK trade. This paper analyzes the overall characteristics, centrality, spatial structure, and vulnerability of the CJK shipping network using the methods of complex network analysis, blocking flow theory, and interruption and deletion of hub ports. The main findings are as follows: 1) The CJK shipping network has a small average path length and clustering coefficient, and its degree distribution follows a power-law distribution, which make the network present obvious characteristics of a Barabási-Albert scale-free. 2) The characteristics of the multi-center point of the CJK shipping network can alleviate traffic pressure. At the same time, the network shows a clear hierarchy in the port transportation system, with cargo transport relying mainly on the ‘hub port-hub port' connection. 3) The CJK shipping network is relatively stable. Compared with ports in Japan and Korea, the main hub ports in China have a greater impact on the stability of the shipping network, in particular those ports of the central coastal region, including Shanghai, Ningbo, and Lianyungang. | GUO Jianke WANG Shaobo WANG Dandan LIU Tianbao | 2017 | Chinese Geographical Science2017，27，5： | 15 |
| 3 | Lyzl4, a novel mouse sperm-related protein, is involved in fertilization显示文摘鸡类型(c 类型) 溶解酵素的角色,原型溶解酵素,在免疫在许多有机体被描绘了。在这研究,我们克隆新奇 c 类型像溶解酵素的基因, Lyzl4,它位于老鼠染色体 9F4 并且与通常认为的信号肽和一个朊酶劈开地点编码了 145 氨基酸。在酵母表示的成熟 recombinant Lyzl4 蛋白质没显示出 bacteriolytic 活动。顺序排列分析证明在 c 类型溶解酵素的 20 不变的残余中的 3 个在 Lyzl4 被改变。改变的氨基酸(D52G ) 之一位于催化领域。Lyzl4 mRNA 有选择地在成年鼠标在睾丸和 epididymis 被表示,与改变越过不同发展阶段的表达式水平。Lyzl4 蛋白质的高水平在 acrosomal 区域和尾巴的主要的片的精子上被发现。在有它的特定的抗体的精子的 Lyzl4 蛋白质的免疫中立化显著地以一种剂量依赖者方式在 vitro 授精百分比减少了,建议 Lyzl4 可能为授精是重要的。 | Ruilin Sun Ruling Shen Jun Li Guojiang Xu Jun Chi Limei Li Jianke Ren Zhugang Wang Jian Fei | 2011 | Acta Biochimica et Biophysica Sinica2011，43，5： | 9 |
| 4 | Influence of Processing Conditions on Microstructure and Mechanical Properties of Large Thin-Wall Centrifugal Ti–6Al–4V Casting显示文摘在这个工作,包括张力的静电干扰和扔的大薄墙的圆柱的 Ti-6Al-4V 的损坏忍耐性质,温度并且热均衡说的紧迫(HIPping ) 在微结构特征和机械性质上处理的模子预热的效果被学习了。试验性的结果显示出那,增加的模子预热从 673 的温度到 873K,扔的微观结构从 Widmanst 的混合物变化 ? tten 和殖民地微观结构到一个主要殖民地。厚墙节的中心比边和薄节有相对粗糙的微观结构。更低的模子预热温度引起更多的孔。HIPping 过程,它不仅有效地减少扔的毛孔而且增加优先的谷物边界结合并且变粗微观结构,对改进扔的韧性必要。由于表面上的氧污染和更好的微观结构,生气的节上的微坚硬的侧面从表面介绍一个减少的趋势给内部。为预热的不同模子的反应层的厚度温度将近一样(~ 450 畃洠捩潲 ' 跍?湩琠敨戠楬摮栠汯? 楷桴愠搠慩敭整 ? 景 ? 0? | Xin Feng Jianke Qiu Yingjie Ma Jiafeng Lei Yuyou Cui Xinhua Wu Rui Yang | 2016 | Journal of Materials Science & Technology2016，32，4： | 9 |
| 5 | PROPAGATION OF LOVE WAVES IN PRESTRESSED PIEZOELECTRIC LAYERED STRUCTURES LOADED WITH VISCOUS LIQUID显示文摘我们调查在压电的分层的结构的起始的应力的效果装载了与的经分解粘滞爱的散、稀释的特征上的液体飘动,它包含薄压电的层完美地结合了到无界的有弹性的底层。在压电的层的起始的应力的效果并且粘滞爱波浪的阶段速度上的液体的系数被分析。数字结果被介绍并且讨论。分析方法和结果能为化学药品和 biosensing 液体传感器的设计是有用的。 | Jianke Du Kai Xian Ji Wang Yook-Kong Yong | 2008 | Acta Mechanica Solida Sinica2008，21，6： | 7 |
| 6 | sLe^(x) expression in invasive micropapillary breast carcinoma is associated with poor prognosis and can be combined with MUC1/EMA as a supplementary diagnostic indicator显示文摘Objective: Mucin 1(MUC1/EMA) and sialyl Lewis X(sLe^(x)) indicate polarity reversal in invasive micropapillary carcinoma(IMPC). The purpose of this study was to evaluate the expression of MUC1/EMA and sLe^(x) and to assess their diagnostic and prognostic value in patients with IMPC.Methods: The expression of sLe^(x) and MUC1/EMA in 100 patients with IMPC and a control group of 89 patients with invasive ductal carcinoma not otherwise specified(IDC-NOS) were analyzed with IHC. Fresh tumor tissues were collected from patients with IMPC or IDC-NOS for primary culture and immunofluorescence analysis.Results: The rate of nodal metastasis was higher in patients with IMPC than those with IDC-NOS, and IMPC cells tended to express more sLe^(x) and MUC1/EMA in the cytomembranes(the stroma-facing surfaces of the micropapillary clusters) than IDC-NOS cells. In IMPC, high cytomembrane expression of sLe^(x), but not MUC1/EMA, indicated poor prognosis. In addition, among the 100 patients with IMPC, 10 patients had sLe^(x)+/EMA-expression patterns, and 8 patients had sLe^(x)-/EMA+ expression patterns. The primary IMPC cells were suspended, non-adherent tumor cell clusters, whereas the primary IDC cells were adherent tumor cells. Immunofluorescence analysis showed that MUC1/EMA and sLe^(x) were co-expressed on the cytomembranes in IMPC cell clusters and in the cytoplasm in IDC-NOS cells.Conclusions: sLe^(x) can be used as a prognostic indicator and can be combined with MUC1/EMA as a complementary diagnostic indicator to avoid missed IMPC diagnosis. | Yawen Song Hui Sun Kailiang Wu Jianke Lyu Jingyue Zhang Feng Gu Yongjie Ma Beibei Shen Chijuan Wang Xiaojiao Chen Jing Xu Weidong Li Fangfang Liu Li Fu | 2021 | Cancer Biology & Medicine2021，18，2： | 7 |
| 7 | Temperature-gradient induced microstructure evolution in heat-affected zone of electron beam welded Ti-6Al-4V titanium alloy显示文摘The heat-affected zone(HAZ) of electron beam welded(EBW) joint normally undergoes a unique heat-treating process consisting of rapid temperature rising and dropping stages, resulting in temperature-gradient in HAZ as a function of the distance to fusion zone(FZ). In the current work,microstructure, elements distribution and crystallographic orientation of three parts(near base material(BM) zone, mid-HAZ and near-FZ) in the HAZ of Ti-6Al-4V alloy were systematically investigated. The microstructure observation revealed that the microstructural variation from near-BM to near-FZ included the reduction of primary α(αp) grains, the increase of transformed β structure(βt) and the formation of various α structures. The rim-α, dendritic α and abnormal secondary α(αs) colonies formed in the mid-HAZ, while the 'ghost' structures grew in the near-FZ respectively. The electron probe microanalyzer(EPMA) and electron back-scattered diffraction(EBSD) technologies were employed to evaluate the elements diffusion and texture evolution during the unique thermal process of welding. The formation of the various α structures in the HAZ were discussed based on the EPMA and EBSD results. Finally, the nanoindentation hardness of 'ghost' structures was presented and compared with nearby βt regions. | Shilin Zhang Yingjie Ma Sensen Huang Sabry S.Youssef Min Qi Hao Wang Jianke Qiu Jiafeng Lei Rui Yang | 2019 | Journal of Materials Science & Technology2019，35，8： | 6 |
| 8 | Latest Triassic to Early Jurassic Thrusting and Exhumation in the Southern Ordos Basin, North China: Evidence from LA-ICP-MS-based Apatite Fission Track Thermochronology显示文摘The contractional structures in the southern Ordos Basin recorded critical evidence for the interaction between Ordos Basin and Qinling Orogenic Collage. In this study, we performed apatite fission track(AFT) thermochronology to unravel the timing of thrusting and exhumation for the Laolongshan-Shengrenqiao Fault(LSF) in the southern Ordos Basin. The AFT ages from opposite sides of the LSF reveal a significant latest Triassic to Early Jurassic time-temperature discontinuity across this structure. Thermal modeling reveals at the latest Triassic to Early Jurassic, a ~50°C difference in temperature between opposite sides of the LSF currently exposed at the surface. This discontinuity is best interpreted by an episode of thrusting and exhumation of the LSF with ~1.7 km of net vertical displacement during the latest Triassic to Early Jurassic. These results, when combined with earlier thermochronological studies, stratigraphic contact relationship and tectono-sedimentary evolution, suggest that the southern Ordos Basin experienced coeval intense tectonic contraction and developed a north-vergent fold-and-thrust belt. Moreover, the southern Ordos Basin experienced a multi-stage differential exhumation during Mesozoic, including the latest Triassic to Early Jurassic and Late Jurassic to earliest Cretaceous thrust-driven exhumation as well as the Late Cretaceous overall exhumation. Specifically, the two thrust-driven exhumation events were related to tectonic stress propagation derived from the latest Triassic to Early Jurassic continued compression from Qinling Orogenic Collage and the Late Jurassic to earliest Cretaceous intracontinental orogeny of Qinling Orogenic Collage, respectively. By contrast, the Late Cretaceous overall exhumation event was related to the collision of an exotic terrain with the eastern margin of continental China at ~100 Ma. | ZHANG Shaohua LIU Chiyang YANG Minghui BAI Jianke WANG Jianqiang | 2018 | Acta Geologica Sinica(English Edition)2018，92，4： | 5 |
| 9 | An analysis of surface acoustic wave propagation in a plate of functionally graded materials with a layered model显示文摘In a homogeneous plate, Rayleigh waves will have a symmetric and anti-symmetric mode regarding to the mid-plane with different phase velocities. If plate properties vary along the thickness, or the plate is of functionally graded material (FGM), the symmetry of modes and frequency behavior will be modified, thus producing dif-ferent features for engineering applications such as amplifying or reducing the velocity and deformation. This kind of effect can also be easily realized by utilizing a layered structure with desired material properties that can produce these effects in terms of velocity and displacements, since Rayleigh waves in a solid with gen-eral material property grading schemes are difficult to analyze with known methods. Solutions from layered structures with exponential and polynomial property grad-ing schemes are obtained from the layered model and comparisons with known analytical results are made to validate the method and examine possible applica-tions of such structures in engineering. | GAO LiMing WANG Ji ZHONG Zheng DU JianKe | 2008 | Science China(Physics,Mechanics & Astronomy)2008，51，2： | 5 |
| 10 | Proteomic analysis of heterosis in the leaves of sorghum-sudangrass hybrids显示文摘 | Pingan Han Xiaoping Lu Fugui Mi Jing Dong Chunlei Xue Jianke Li Bin Han Xiaoyu Zhang | 2016 | Acta Biochimica et Biophysica Sinica2016，48，2： | 5 |
| 11 | A Comparison in Laser Precision Drilling of Stainless Steel 304 with Nanosecond and Picosecond Laser Pulses显示文摘Precision drilling with picosecond laser has been advocated to significantly improve the quality of micro-holes with reduced recast layer thickness and almost no heat affected zone.However,a detailed comparison between nanosecond and picosecond laser drilling techniques has rarely been reported in previous research.In the present study,a series of micro-holes are manufactured on stainless steel 304 using a nanosecond and a picosecond laser drilling system,respectively.The quality of the micro-holes,e.g.,recast layer,micro-crack,circularity,and conicity,etc,is evaluated by employing an optical microscope,an optical interferometer,and a scanning electron microscope.Additionally,the micro-structure of the samples between the edges of the micro-holes and the parent material is compared following etching treatment.The researching results show that a great amount of spattering material accumulated at the entrance ends of the nanosecond laser drilled micro-holes.The formation of a recast layer with a thickness of^25μm is detected on the side walls,associated with initiation of micro-cracks.Tapering phenomenon is also observed and the circularity of the micro-holes is rather poor.With regard to the micro-holes drilled by picosecond laser,the entrance ends,the exit ends,and the side walls are quite smooth without accumulation of spattering material,formation of recast layer and micro-cracks.The circularity of the micro-holes is fairly good without observation of tapering phenomenon.Furthermore,there is no obvious difference as for the micro-structure between the edges of the micro-holes and the parent material.This study proposes a picosecond laser helical drilling technique which can be used for effective manufacturing of high quality micro-holes. | ZHANG Hongyu DI Jianke ZHOU Ming YAN Yu | 2014 | Chinese Journal of Mechanical Engineering2014，27，5： | 5 |
| 12 | A theoretical model for the intrusion of the Kuroshio across the continental shelf of East China Sea显示文摘Here we use a two-layer model to study the dynamics of the intrusion of the Kuroshio onto the continental shelf. Results show that the interaction of the Kuroshio water and shelf water produces a stable upwelling zone above 100 m depth northeast of Taiwan, which provides a dynamical explanation to the presence of the cold core previously observed by satellite. The affected shelf water from the interaction has an onshore portion, which turns right and becomes a northward alongshore flow when it moves closer to shore. This implies that the Kuroshio water cannot penetrate deep onto the inner part of the continental shelf, but it generates a strong northward jet that is formed mainly by the shelf water. | ZHANG QingHua QU YuanYuan LI JianKe YIN XunQiang | 2015 | Science China Earth Sciences2015，58，12： | 4 |
| 13 | PARALLEL FINITE ELEMENT ANALYSIS OF HIGH FREQUENCY VIBRATIONS OF QUARTZ CRYSTAL RESONATORS ON LINUX CLUSTER显示文摘石英水晶共鸣器是典型压电的声学的波浪设备因为有在无线电频率(RF ) 的机械颤动频率的频率控制应用变化。颤动和变丑的精确分析通常在共鸣器设计和改进过程被要求。考虑包括电极,增长,象温度那样的偏爱地,起始的压力,和加速的存在。自然地,有限元素方法是为有多物理自然的如此的 a coupled 问题的唯一的有效工具。主要挑战是结果的线性方程的极其大的尺寸。为这个原因,我们一直在采用 Mindlin 板方程减少计算困难。另外,我们不得不在 Linux 簇上利用并行计算技术,它现在为学术、工业的应用程序是广泛地可得到的,到改进计算效率。我们的研究的一般原则是使用开放源代码软件部件和公共领域技术在 Linux 簇上为开发者和用户减少费用。我们明确地以一个网孔发电机开始因为矩形、圆形的类型的石英水晶共鸣器,和 Mindlin 板方程为有限元素分析被实现。象并行处理,处理的稀少的矩阵,和最近的特征值抽取包裹一样的计算技术集成于程序。它从我们簇上的这些算法和方法的联合能显著地满足记忆要求和还原剂计算时间的计算是清楚的。 | Ji Wang Yu Wang Wenke Hu Wenhua Zhao Jianke Du Dejin Huang | 2008 | Acta Mechanica Solida Sinica2008，21，6： | 4 |
| 14 | A two-dimensional analysis of surface acoustic waves in finite elastic plates with eigensolutions显示文摘It is generally known that surface acoustic waves, or Rayleigh waves, have different mode shapes in infinite plates. To be precise, there are both exponentially decaying and growing components in plates appearing in pairs, representing symmetric and antisymmentric modes in a plate. As the plate thickness increases, the combined modes will approach the Rayleigh mode in a semi-infinite solid, exhibiting surface acoustic wave deformation and velocity. In this study, the two-dimensional theory for surface acoustic waves in finite plates is extended to include the exponentially growing modes in the expansion function. With these extra equations, we study the surface acoustic waves in a plate with different thickness to examine the coupling of the exponentially decaying and growing modes. It is found that for small thick- ness, the two groups of waves are strongly coupled, showing the significance of including the effect of thickness in analysis. As the thickness increases to certain values, such as more than five wavelengths, the exponentially decaying modes alone will be able to predict vibrations of surface acoustic wave modes accurately, thus simplifying the equations and solutions significantly. | WANG Ji DU JianKe PAN QiaoQiao | 2007 | Science China(Physics,Mechanics & Astronomy)2007，50，5： | 4 |
| 15 | Love wave propagation in layered magneto- electro-elastic structures显示文摘An analytical approach was taken to investigate Love wave propagation in a layered magneto-electro-elastic structure, where a piezomagnetic (or piezoelectric) mate-rial thin layer was bonded to a semi-infinite piezoelectric (or piezomagnetic) sub-strate. Both piezoelectric and piezomagnetic ceramics were polarized in the anti-plane (z-axis) direction. The analytical solution of dispersion relations was obtained for magneto-electrically open and short boundary conditions. The phase velocity, group velocity, magneto-electromechanical coupling factor, electric po-tential, and magnetic potential were calculated and discussed in detail. The nu-merical results show that the piezomagnetic effects have remarkable effect on the propagation of Love waves in the layered piezomagnetic/piezoelectric structures. | DU JianKe JIN XiaoYing WANG Ji | 2008 | Science China(Physics,Mechanics & Astronomy)2008，51，6： | 3 |
| 16 | Improved method to raise polyclonal antibody using enhanced green fluorescent protein transgenic mice显示文摘Recombinant 熔化蛋白质广泛地被用作抗原对目标蛋白质的 epitope 提起抗体。然而,在产生抗血清的伴随物反搬运人抗体减少需要的抗体的生产并且引起讨厌的非特定的有免疫力的反应。这被建议搬运人蛋白质转基因的动物能被用来解决这个问题。验证这个假设,提高的绿荧光灯的蛋白质(EGFP ) 转基因的老鼠被生产。由与熔化蛋白质 His6HAtag-EGFP 使老鼠免疫,我们证明从转基因的老鼠的抗血清从野类型的老鼠与那相比对 EGFP 对 His6HA 标签和更低的 titer 抗体有更高的 titer 抗体。因此,这份报告描述一个改进方法用能在抗体准备有申请潜力的 EGFP 转基因的老鼠提起高 titer 反肽 polyclonal 抗体。 | Jianke Ren Long Wang Guoxiang Liu Wen Zhang Zhejin Sheng Zhugang Wang Jian Fei | 2008 | Acta Biochimica et Biophysica Sinica2008，40，2： | 3 |
| 17 | Fatigue crack tip plastic zone ofα+βtitanium alloy with Widmanstatten microstructure显示文摘The recent studies had focused on the fatigue crack propagation behaviors of α+β titanium alloys with Widmanstatten microstructure. The fascinated interest of this type of microstructure is due to the superior fatigue crack propagation resistance and fracture toughness as compared to other microstructures,which was believed to be related to the fatigue crack tip plastic zone(CTPZ). In this study, the plastic deformation in fatigue CTPZ of Ti-6 A1-4 V titanium alloy with Widmanstatten microstructure was characterized by scanning electron microscope(SEM) and electron backscatter diffraction(EBSD). The results showed that large-scale slipping and deformation twinning were generated in fatigue CTPZ due to the crystallographic feature of the Widmanstatten microstructure. The activation of twinning was related to the rank of Schmid factor(SF) and the diversity of twin variants developing behaviors reflected the influence of SF rank. The sizes of CTPZ under different stress intensity factors(K) were examined by the white-light coherence method, and the results revealed that the range of the plastic zone is enlarged with the increasing K(or crack length), while the plastic strain decreased rapidly with the increasing distance from the crack surface. The large-scale slipping and deformation twinning in Widmannstatten microstructure remarkably expanded the range of fatigue CTPZ, which would lead to the obvious larger size of the observed CTPZ than that of the theoretically calculated size. | Yingjie Ma Sabry S.Youssef Xin Feng Hao Wang Sensen Huang Jianke Qiu Jiafeng Lei Rui Yang | 2018 | Journal of Materials Science & Technology2018，34，11： | 3 |
| 18 | Effects of heating rate on the alloy element partitioning and mechanical properties in equiaxedα+βTi-6Al-4V alloy显示文摘The effects of heating rate on the alloy element partitioning and mechanical properties during the phase transformation ofα→βin Ti-6Al-4 V alloy under solution treatment have been investigated by the experiments and phase field simulations,which reveal the evolutions of microstructure and compositions at the non-equilibrium state and well verify the experimental results.The specific results indicate that the compositions measured through electron probe micro-analysis(EPMA)under a lower heating rate are close to the equilibrium ones corresponding to the solution temperature.Heating up to the target solution temperature,as the heating rate increases,the Al content decreases and V increases in the primaryα(α_(p))grain with a larger size,the volume fraction ofα_(p)increases and the composition gradient betweenα_(p)andβphases gets steeper.The interrelated relationship among the diffusion,compositions,solution temperature and free energy of the system has been discussed in detail.Moreover,increasing the heating rate(~20.0 K/min)may help to improve the mechanical properties of the alloy by mainly adjusting theα_(p)/β;volume fractions,α_(p)particle size and secondaryα(α_(p))size during the process of heating up to the solution temperature.These results may shed some light on the optimization of the knowledge-based heat treatment route. | Jinhu Zhang Hongtao Ju Haisheng Xu Liang Yang Zhichao Meng Chen Liu Ping Sun Jianke Qiu Chunguang Bai Dongsheng Xu Rui Yang | 2021 | Journal of Materials Science & Technology2021，，35： | 2 |
| 19 | Thickness-Shear Frequencies of an Infinite Quartz Plate with Graded Material Properties Across the Thickness显示文摘For quartz crystal resonators of thickness-shear type,the vibration frequency and mode shapes reflect the basic material and structural properties of a quartz crystal plate and the variation with time under attack by erosive gases and liquids that can cause surface and internal degradation in a graded form.Such gradual effects,in turn,will change surface conditions through elastic constants and stiffness and more importantly the gradient of such properties across the thickness with certain depth.Now we study the thickness-shear vibrations of functionally graded material plates with properties of the popular AT-cut quartz crystal varying across the thickness in a general pattern represented by both sine and cosine components of the thickness.The vibration solutions are obtained through the Fourier expansion of the plate deformation. | Rongxing Wu Wenliang Zhang Tingfeng Ma Jianke Du Ji Wang | 2020 | Acta Mechanica Solida Sinica2020，33，3： | 2 |
| 20 | A chromosome-level genome assembly of rugged rose (Rosa rugosa) provides insights into its evolution, ecology, and floral characteristics显示文摘Rosa rugosa,commonly known as rugged rose,is a perennial ornamental shrub.It produces beautiful flowers with a mild fragrance and colorful seed pods.Unlike many other cultivated roses,R.rugosa adapts to a wide range of habitat types and harsh environmental conditions such as salinity,alkaline,shade,drought,high humidity,and frigid temperatures.Here,we produced and analyzed a high-quality genome sequence for R.rugosa to understand its ecology,floral characteristics and evolution.PacBio HiFi reads were initially used to construct the draft genome of R.rugosa,and then Hi-C sequencing was applied to assemble the contigs into 7 chromosomes.We obtained a 382.6Mb genome encoding 39,704 protein-coding genes.The genome of R.rugosa appears to be conserved with no additional whole-genome duplication after the gamma whole-genome triplication(WGT),which occurred~100 million years ago in the ancestor of core eudicots.Based on a comparative analysis of the high-quality genome assembly of R.rugosa and other high-quality Rosaceae genomes,we found a unique large inverted segment in the Chinese rose R.chinensis and a retroposition in strawberry caused by post-WGT events.We also found that floral development-and stress response signaling-related gene modules were retained after the WGT.Two MADS-box genes involved in floral development and the stress-related transcription factors DREB2A-INTERACTING PROTEIN 2(DRIP2)and PEPTIDE TRANSPORTER 3(PTR3)were found to be positively selected in evolution,which may have contributed to the unique ability of this plant to adapt to harsh environments.In summary,the high-quality genome sequence of R.rugosa provides a map for genetic studies and molecular breeding of this plant and enables comparative genomic studies of Rosa in the near future. | Fei Chen Liyao Su Shuaiya Hu Jia-Yu Xue Hui Liu Guanhua Liu Yifan Jiang Jianke Du Yushan Qiao Yannan Fan Huan Liu Qi Yang Wenjie Lu Zhu-Qing Shao Jian Zhang Liangsheng Zhang Feng Chen Zong-Ming(Max)Cheng | 2021 | Horticulture Research2021，8，1： | 2 |
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