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| 1 | Washed microbiota transplantation vs.manual fecal microbiota transplantation:clinical findings,animal studies and in vitro screening显示文摘Fecal microbiota transplantation(FMT)by manual preparation has been applied to treat diseases for thousands of years.However,this method still endures safety risks and challenges the psychological endurance and acceptance of doctors,patients and donors.Population evidence showed the washed microbiota preparation with microfiltration based on an automatic purification system followed by repeated centrifugation plus suspension for three times significantly reduced FMT-related adverse events.This washing preparation makes delivering a precise dose of the enriched microbiota feasible,instead of using the weight of stool.Intraperitoneal injection in mice with the fecal microbiota supernatant obtained after repeated centrifugation plus suspension for three times induced less toxic reaction than that by the first centrifugation following the microfiltration.The toxic reactions that include death,the change in the level of peripheral white blood cells,and the proliferation of germinal center in secondary lymphoid follicles in spleen were noted.The metagenomic next-generation sequencing(NGS)indicated the increasing types and amount of viruses could be washed out during the washing process.Metabolomics analysis indicated metabolites with pro-inflammatory effects in the fecal microbiota supernatant such as leukotriene B4,corticosterone,and prostaglandin G2 could be removed by repeated washing.Near-infrared absorption spectroscopy could be served as a rapid detection method to control the quality of the washingprocess.In conclusion,this study for the first time provides evidence linking clinical findings and animal experiments to support that washed microbiota transplantation(WMT)is safer,more precise and more quality-controllable than the crude FMT by manual. | Ting Zhang Gaochen Lu Zhe Zhao Yafei Liu Quan Shen Pan Li Yaoyao Chen Haoran Yin Huiquan Wang Cicilia Marcella Bota Cui Lei Cheng Guozhong Ji Faming Zhang | 2020 | Protein & Cell2020，11，4： | 44 |
| 2 | Mitochondrial PKM2 regulates oxidative stress-induced apoptosis by stabilizing Bcl2显示文摘 | Ji Liang Ruixiu Cao Xiongjun Wang Yajuan Zhang Pan Wang Hong Gao Chen Li Fan Yang Rong Zeng Ping Wei Dawei Li Wenfeng Li Weiwei Yang | 2017 | Cell Research2017，27，3： | 39 |
| 3 | Fecal microbiota transplantation cured epilepsy in a case with Crohn's disease: The first report显示文摘Fecal microbiota transplantation(FMT)is a promising strategy that involves reconstruction of gut microbiota.Recently,it has been considered as a treatment of Crohn’s disease(CD)and certain neurological diseases.Here,to the best of our knowledge,we report the first case that used FMT to achieve remission of intestinal and neurological symptoms in a girl with CD and a 17-year history of epilepsy.During the 20 mo of follow-up,FMT has proved its efficacy in preventing relapse of seizures after withdrawing the antiepileptic drugs.Furthermore,this finding highlights the role of microbiota-gut-brain axis and inspires a novel treatment for epilepsy through remodeling gut microbiota. | Zhi He Bo-Ta Cui Ting Zhang Pan Li Chu-Yan Long Guo-Zhong Ji Fa-Ming Zhang | 2017 | World Journal of Gastroenterology2017，23，19： | 37 |
| 4 | Paleomagnetic data from Early Cretaceous volcanic rocks of West Liaoning:Evidence for intracontinental rotation显示文摘Detailed rock magnetic studies of 55 lavas from Yixian and Fuxin area, West Liaoning, show the primary carriers of remanence to be pseudo-single domain ti-tanomagnetite. K/Ar dating indicates that the volcanic sequence spans 93 to 133 Ma. Stepwise thermal demagnetization successfully isolated well-defined characteristic magnetization (ChRM) in all lavas thermal-treated above 250℃ The mean paleodirections are D/I=5.9°/58.8° (α95 = 2.9°) and D/I =179.2°/-59.9° (α95 = 5.2°) for 27 normally magnetized flows and 28 reversibly magnetized flows, respectively. It indicates that since the Early Cretaceous there is no significant horizontal movement and rotation between the Yixian-Fuxin area and Eurasia. However, Korea Peninsula may have undergone a clockwise rotation of 33.9° relative to the Yixian-Fuxin area during the Cretaceous. On the basis of characteristics of hotspot origins (core-mantle boundary or upper mantle), the clockwise rotation of Korea Peninsula relative to Eurasia is assumed to be | ZHU Rixiang SHAO Ji’an PAN Yongxin SHI Ruiping SHI Guanghai LI Darning | 2002 | Chinese Science Bulletin2002，47，21： | 31 |
| 5 | Application of surface-enhanced laser desorption/ionization time-of-flight-based serum proteomic array technique for the early diagnosis of prostate cancer显示文摘瞄准:由蛋白质薄片和生物信息学识别前列腺癌症(PCa ) 的浆液简历标记。方法:从 83 个 PCa 病人和 95 个健康的人的浆液样品从在长春屏蔽的一个团被拿,中国。蛋白质介绍用提高表面的激光解吸附作用 / 电离 time-of-flight 团分光术(SELDI-TOF MS ) 被执行。系列的数据用二个生物信息学工具被分析。结果:十八浆液微分蛋白质与控制组相比在 PCa 组被识别(P <
0.01 ) 。在在 PCa 组的更低的浆液水平在更高的浆液水平和 14 蛋白质有四蛋白质。使用了一个八蛋白质的集体模式的一个决定树分类算法被开发正确地分类样品。92.0% 的敏感和为学习组的 96.7% 的特性被比较 PCa 和控制组获得。结论:我们识别了 PCa 的新浆液简历标记。SELDI-TOF MS 结合了树分类算法将为 PCa 的早诊断提供一条高度精确、创新的途径的一个决定。 | Yu-Zhuo Pan Xue-Yuan Xiao Dan Zhao Guo-Yi Ji Yang Li Bao-Xue Yang Da-Cheng He Xue-Jian Zhao | 2006 | Asian Journal of Andrology2006，8，1： | 32 |
| 6 | Improved survival of porcine acute liver failure by a bioartificial liver device implanted with induced human functional hepatocytes显示文摘 | Xiao-Lei Shi Yimeng Gao YupengYan HuchengMa Lulu Sun Pengyu Huang Xuan Ni Ludi Zhang Xin Zhao Haozhen Ren Dan Hu Yan Zhou Feng Tian Yuan Ji Xin Cheng Guoyu Pan Yi-Tao Ding LijianHui | 2016 | Cell Research2016，26，2： | 31 |
| 7 | Main controlling factors and enrichment area evaluation of shale gas of the Lower Paleozoic marine strata in south China显示文摘The Lower Paleozoic shale in south China has a very high maturity and experienced strong tectonic deformation. This character is quite different from the North America shale and has inhibited the shale gas evaluation and exploration in this area. The present paper reports a comprehensive investigation of maturity, reservoir properties,fluid pressure, gas content, preservation conditions, and other relevant aspects of the Lower Paleozoic shale from the Sichuan Basin and its surrounding areas. It is found that within the main maturity range(2.5 %3.5 %) is considered a high risk for shale gas exploration. Taking the southern area of the Sichuan Basin and the southeastern area of Chongqing as examples of uplifted/folded and faulted/folded areas, respectively, geological models of shale gas content and loss were proposed.For the uplifted/folded area with a simple tectonic deformation, the shale system(with a depth >2000 m) has largely retained overpressure during uplifting without a great loss of gas, and an industrial shale gas potential is generally possible. However, for the faulted/folded area with a strong tectonic deformation, the sealing condition of the shalesystem was usually destroyed to a certain degree with a great loss of free gas, which decreased the pressure coefficient and resulted in a low production capacity. It is predicted that the deeply buried shale(>3000 m) has a greater gas potential and will become the focus for further exploration and development in most of the south China region(outside the Sichuan Basin). | Xian-Ming Xiao Qiang Wei Hai-Feng Gai Teng-Fei Li Mao-Lin Wang Lei Pan Ji Chen Hui Tian | 2015 | Petroleum Science2015，12，4： | 27 |
| 8 | Experimental and clinical study of influence of high-frequency electric surgical knives on healing of abdominal incision显示文摘瞄准:在腹壁切口愈合上学习高周波的电的外科的刀的影响。方法:240 只白鼠被划分成 10 (0 ) , 10 (2 ) , 10 (5 ) ,并且 10 (8 ) 组和腹部手术的老鼠模型被分别地使用电的外科的刀和普通口针劝诱。然后,他们分别地被给生理盐水和 0.2 mL 的皮下注射在 10 的集中(2 ) 的埃希氏杆菌属关口 i,葡萄球菌 aureus 和绿脓假单胞菌的量的混合, 10 (5 ) 并且 10 (8 ) 。根据动物实验,经历腹部手术(上面的类型 II ) 的 220 个病人随机被分配进跟随三个组之一:电热刀(EK, 93 个盒子) ,电凝法(EC, 55 个盒子) 并且控制(72 个盒子) 。高周波的电的外科的刀习惯于为在 EK 的血壅滞的腹的纸巾和电凝法组织的 dissect。普通口针和电凝法在 EC 组被使用。普通口针和系住的丝缝线在控制被使用。结果:在除了组 10 的所有组(0 ) ,电的外科的刀做的 incisional 创伤的感染率与普通口针比那显著地高。而且,在组有有效差量 10 (2 ) , 10 (5 ) ,并且 10 (8 )(P<0.05 ) ,然而并非(0 )(P>0.05 ) 在组, 10 在 EK 和 EC 之间组织。临床的研究显示出在控制组在 EK,在 EC 的 11 个盒子(16.36%) 和 2 个盒子(2.86%) 在 16 种情况(17.20%) 中愈合的一处推迟的创伤。在 EK 和控制之间的有效差量组织(chi2 = 8.57, P<0.01 ) ,并且在 EC 和控制组之间(chi2 = 5.66, P<0.05 ) 被观察,然而并非在 EK 和 EC 之间(chi2 = 0.017, P>0.05 ) 。结论:高周波的电的刀可以显著地推迟腹壁切口愈合。它的申请应该被最小化以便减少手术后的复杂并发症的可能性。 | Guang-Wei Ji Yuan-Zhi Wu Xu Wang Hua-Xiong Pan Ping Li Wan-Ying Du Zhi Qi An Huang Li-Wei Zhang Li Zhang Wen Chen Guang-Hua Liu Hui Xu Quan Li Ai-Hua Yuan Xiao-Ping He Guo-Hua Mei | 2006 | World Journal of Gastroenterology2006，12，25： | 23 |
| 9 | 2016 Chinese expert consensus and guidelines for the diagnosis and treatment of papillary thyroid microcarcinoma显示文摘The incidence of papillary thyroid carcinoma(PTC)has exponentially increased in recent years.Papillary thyroid microcarcinoma(PTMC)accounts for the majority of the reported cases of PTC.The debates and crucial issues in PTMC management have received researchers'attention.To further improve the clinical management of PTMC in China, | Ming Gao Minghua Ge Qinghai Ji Ruochuan Cheng Hankui Lu Haixia Guan Li Gao Zhuming Guo Tao Huang Xiaoming Huang Xiaoming Li Yansong Lin Qinjiang Liu Xin Ni Yi Pan Jianwu Qin Zhongyan Shan Hui Sun Xudong Wang Zhengang Xu Yang Yu Daiwei Zhao Naisong Zhang ShengZhang Ying Zheng Jingqiang Zhu Dapeng Li Xiangqian Zheng 无 | 2017 | Cancer Biology & Medicine2017，14，3： | 23 |
| 10 | Sirtuin 1 alleviates endoplasmic reticulum stress-mediated apoptosis of intestinal epithelial cells in ulcerative colitis显示文摘BACKGROUND Sirtuin 1(SIRT1)is a nicotinamide adenine dinucleotide(NAD+)-dependent protein deacetylase that is involved in various diseases,including cancers,metabolic diseases,and inflammation-associated diseases.However,the role of SIRT1 in ulcerative colitis(UC)is still confusing.AIM To investigate the role of SIRT1 in intestinal epithelial cells(IECs)in UC and further explore the underlying mechanisms.METHODS We developed a coculture model using macrophages and Caco-2 cells.After treatment with the SIRT1 activator SRT1720 or inhibitor nicotinamide(NAM),the expression of occludin and zona occludens 1(ZO-1)was assessed by Western blot analysis.Annexin V-APC/7-AAD assays were performed to evaluate Caco-2 apoptosis.Dextran sodium sulfate(DSS)-induced colitis mice were exposed to SRT1720 or NAM for 7 d.Transferase-mediated dUTP nick-end labeling(TUNEL)assays were conducted to assess apoptosis in colon tissues.The expression levels of glucose-regulated protein 78(GRP78),CCAAT/enhancerbinding protein homologous protein(CHOP),caspase-12,caspase-9,and caspase-3 in Caco-2 cells and the colon tissues of treated mice were examined by quantitative real-time PCR and Western blot.RESULTS SRT1720 treatment increased the protein levels of occludin and ZO-1 and inhibited Caco-2 apoptosis,whereas NAM administration caused the opposite effects.DSS-induced colitis mice treated with SRT1720 had a lower disease activity index(P<0.01),histological score(P<0.001),inflammatory cytokine levels(P<0.01),and apoptotic cell rate(P<0.01),while exposure to NAM caused the opposite effects.Moreover,SIRT1 activation reduced the expression levels of GRP78,CHOP,cleaved caspase-12,cleaved caspase-9,and cleaved caspase-3 in Caco-2 cells and the colon tissues of treated mice.CONCLUSION SIRT1 activation reduces apoptosis of IECs via the suppression of endoplasmic reticulum stress-mediated apoptosis-associated molecules CHOP and caspase-12.SIRT1 activation may be a potential therapeutic strategy for UC. | Meng-Ting Ren Meng-Li Gu Xin-Xin Zhou Mo-Sang Yu Hang-Hai Pan Feng Ji Chen-Yan Ding | 2019 | World Journal of Gastroenterology2019，25，38： | 20 |
| 11 | Inhibition of long non-coding RNA TUG1 on gastric cancer cell transference and invasion through regulating and controlling the expression of miR-144/c-Met axis显示文摘Objective: To discuss the expression of long noncoding RNA TUG1(lnc RNA-TUG1) in gastric carcinoma(GC) and its effects on the transferring and invading capacity of gastric carcinoma cells. Methods: Forty cases of carcinoma tissue and para-carcinoma tissue were selected from GC patients who underwent surgical removal in Zhejiang Provincial Hospital of Chinese Traditional Medicine and Wenzhou Central Hospital from January, 2013 to December, 2014; the expressing level of lnc RNA-TUG1 in GC and para-C tissues was detected by applying the q RT-PCR technique. The correlation between lnc RNA-TUG1 expression and patients' clinical data was classified and analyzed. SGC-7901 cells were transfected using lnc RNA-TUG1 specific si RNA. Changes of the transferring and invading capacity of si RNAtransfected SGC-7901 cells were scratch-tested and transwell-detected. q RT-PCR was applied to detect the expression level of micro RNA-144 after lnc RNA-TUG1 was silenced. Changes of c-Met m RNA and protein expressions was detected by q RT-PCR and western-blot test. Results: The expression level of lnc RNA-TUG1 in GC tissue was significant higher than that in para-C tissue(P<0.05) and the high expression level of lnc RNA-TUG1 in GC tissue was significantly correlated with tumor lymph nodes metastasis and advance TNM phasing(P<0.05). The transferring and invading capacity of SGC-7901 cells was highly inhibited after being transfected by lnc RNA-TUG1 specific si RNA(P<0.05). The results of q RT-PCR and western-blot proved that the expression of micro RNA-144 was significantly boosted and the expression level of c-Met m RNA and protein was inhibited after lnc RNA-TUG1 was silenced(P<0.05). Conclusions: lnc RNA-TUG1 shows an up-regulated expression in GC tissue and that bears a correlation with clinicopathological features of malignant tumor. lnc RNATUG1 can promote the transferring and invading capacity of GC by inhibiting the pathway of micro RNA-144/c-Met. | Ting-Ting Ji Xuan Huang Jie Jin Sheng-Hua Pan Xiao-Ju Zhuge | 2016 | Asian Pacific Journal of Tropical Medicine2016，9，5： | 20 |
| 12 | Comparative QTL Mapping of Resistance to Gray Leaf Spot in Maize Based on Bioinformatics显示文摘The integration QTL map for gray leaf spot resistance in maize was constructed by compiling a total of 57 QTLs available with genetic map IBM2 2005 neighbors as reference. Twenty-six 'real QTLs' and seven consensus QTLs were identified by refining these 57 QTLs using overview and meta-analysis approaches. Seven consensus QTLs were found on chromosomes 1.06, 2.06, 3.04, 4.06, 4.08, 5.03, and 8.06, and the map coordinates were 552.53, 425.72, 279.20, 368.97, 583.21, 308.68 and 446.14 cM, respectively. Using a synteny conservation approach based on comparative mapping between the maize genetic map and rice physical map, a total of 69 rice and maize resistance genes collected from websites Gramene and MaizeGDB were projected onto the maize genetic map IBM2 2005 neighbors, and 2 (Rgene32, ht1), 4 (Rgene5, rp3, scmv2, wsm2), and 4 (ht2, Rgene6, Rgene8 and Rgene7) positional candidate genes were found in three consensus QTLs on chromosomes 2.06, 3.04, and 8.06, respectively. The results suggested that the combination of meta-analysis of gray leaf spot in maize and sequence homologous comparison between maize and rice could be an efficient strategy for identifying major QTLs and corresponding candidate genes for the gray leaf spot. | SHI Li-yu LI Xin-hai HAO Zhuan-fang XIE Chuan-xiao JI Hai-lian LUE Xiang-ling ZHANG Shi-huang PAN Guang-tang | 2007 | Agricultural Sciences in China2007，6，12： | 19 |
| 13 | The Association between Lower Incisal Inclination and Morphology of the Supporting Alveolar Bone—A Cone-Beam CT Study显示文摘Aim To investigate the relationship between the positioning of the lower central incisor and physical morphology of the surrounding alveolar bone. Methodology Thirty-eight patients (18 males, 20 females), with mean age of 13.4 years, were included in this study. As part of orthodontic treatment planning the patients were required to take dental Cone-beam CT (CBCT) covering the region of lower incisors, the surrounding alveolar bone and the mandibular symphysis. The cephalometric parameters were designed and measured to indicate the inclination of lower central incisor and physical morphology of the adjacent alveolar bone. Computer-aided descriptive statistical analysis was performed using SPSS 15.0 software package for Windows. A correlation analysis and a linear regression analysis between the incisor inclination and the alveolar bone morphology were performed. Results Significant positive correlations were found between the lower central incisor inclination and the morphological contour of the alveolar bone (P<0.05). The lower central incisor root apex was closer to the lingual alveolar crest when it was buccally inclined. Conclusion The morphology of the alveolar bone may be affected by incisal inclination. | Quan Yu Xiao-gang Pan Guo-ping Ji Gang Shen | 2009 | International Journal of Oral Science2009，1，4： | 19 |
| 14 | Safety of once‐daily insulin detemir in patients with type 2 diabetes treated with oral hypoglycemic agents in routine clinical practice (在常规临床实践中使用口服降糖药治疗的2型糖尿病患者每日一次使用地特胰岛素治疗的安全性)显示文摘 | Stuart Ross Grzegorz Dzida Qiuhe Ji Marcel Kaiser Robert Ligthelm Luigi Meneghini Avideh Nazeri Domingo Orozco‐Beltran Changyu Pan Anne Louise Svendsen | 2014 | Journal of Diabetes2014，，3： | 18 |
| 15 | Bioactivity of five components of Chinese herbal formula Jiangzhi granules against hepatocellular steatosis显示文摘OBJECTIVE: This study aims to evaluate the bioactivity of five components of the traditional Chinese medicine complex prescription Jiangzhi granules against hepatocellular steatosis. METHODS: The five major components, including protopanaxadiol, tanshinone IIA, emodin, chlorogenic acid, and nuciferine, were extracted from Jiangzhi granules. Their cytotoxicity was assessed to determine the safe dose of each component for HepG2 cells. HepG2 cellular steatosis was induced using 1 mmol/L of free fatty acids (FFAs) for 24 h, and then treated with each component at high, intermediate, and low doses (500, 50, and 5 μmol/L), respectively for another 24 h. The effects on HepG2 steatosis were observed directly under optical phase microscopy, or through oil red O staining and Nile red assays. In addition, the levels of reactive oxygen species (ROS) in the steatotic HepG2 cells with and without high-dose protopanaxadiol treatment were measured using fluorescent dye 2′,7′-dichlorodihydrofluorescein diacetate staining. RESULTS: No obvious cytotoxicity was observed in the HepG2 cells incubated with each of the five components at up to 500 μmol/L. At 24 h after incubation with FFAs, the HepG2 cells swelled and many lipid droplets accumulated. The lipid content was attenuated after 24 h of incubation with protopanaxadiol, tanshinone IIA, and emodin at 500 or 50 μmol/L (P<0.05), especially with 500 μmol/L protopanaxadiol (P<0.01). In addition, the ROS level was elevated in steatotic cells, but decreased after intervention with 500 μmol/L protopanaxadiol (P<0.05). CONCLUSION: Protopanaxadiol, tanshinone IIA, and emodin alleviate hepatocellular steatosis in a dose-dependent manner, and oxidative stress regulation may partially contribute to the effects of protopanaxadiol. | Hai-yan Song Li Zhang Jie-lu Pan Li-li Yang Guang Ji | 2013 | Journal of Integrative Medicine2013，11，4： | 17 |
| 16 | Recurrent gain-of-function USP8 mutations in Cushing's disease显示文摘库欣的疾病,引起过量皮质醇生产的也已知的同样促肾上腺皮质的荷尔蒙(ACTH )-secreting 垂体腺瘤(舞步) ,占多达 85%corticotrophin 依赖的库欣的症候群盒子。然而,在这疾病的基因改变是不清楚的。这里,我们执行了从 12 秘密 ACTH 舞步导出并且匹配血样品的 DNA 定序的 whole-exome,它在候选人基因揭示了体的变化的三种类型, USP8 (编码 ubiquitin 特定的朊酶 8 ) ,专门在 exon 14 在 12 秘密 ACTH 舞步中的 8 个。我们进一步评估了体的 USP8 变化在另外由定序的 Sanger 的 258 舞步。进一步定序指向在 108 秘密 ACTH 舞步(62.04%) 中的 67 个识别了 USP8 变体的 17 种类型的一个总数。然而,任何一个都没在舞步的另外的类型这些变化被检测(n = 150 ) 。这些变化在 USP8 的 14-3-3 绑定主题以内聚集并且破坏在 USP8 和 14-3-3 蛋白质之间的相互作用,导致保护 EGFR 免受 lysosomal 降级的伤害的一个提高的能力。因此,有 EGFR 表示,提高的 EGFR 蛋白质丰富和 mRNA 表示的更高的发生 POMC 铺平的变异的 USP8 显示的舞步,它编码 ACTH 的先锋。有变异的 USP8 的舞步在尺寸是显著地更小的并且比野类型的舞步有更高的 ACTH 生产。在里面通过手术 resected 主要变异 USP8 的肿瘤房间, USP8 击倒或堵住的 EGFR 有效地稀释 ACTH 分泌物。一起拿,体的 gain-of-function USP8 变化是普通的并且在库欣的疾病贡献 ACTH 生产过剩。USP8 或 EGFR 的抑制为对待变异 USP8 的 corticotrophin 腺瘤是有希望的。我们的学习在库欣的疾病加亮潜在地功能的变异的基因并且提供卓见进这疾病的治疗学。 | Zeng-Yi Ma Zhi-Jian Song Jian-Hua Chen Yong-Fei Wang Shi-Qi Li Liang-Fu Zhou Ying Mao Yi-Ming Li Rong-Gui Hu Zhao-Yun Zhang Hong-Ying Ye Ming Shen Xue-Fei Shou Zhi-Qiang Li Hong Peng Qing-Zhong Wang Dai-Zhan Zhou Xiao-Lan Qin Jue Ji Jie Zheng Hong Chen Yin Wang Dao-Ying Geng Wei-Jun Tang Chao-Wei Fu Zhi-Feng Shi Yi-Chao Zhang Zhao Ye Wen-Qiang He Qi-Lin Zhang Qi-Sheng Tang Rong Xie Jia-Wei Shen Zu-Jia Wen Juan Zhou Tao Wang Shan Huang Hui-Jia Qiu Ni-Dan Qiao Yi Zhang Li Pan Wei-Min Bao Ying-Chao Liu Chuan-Xin Huang Yong-Yong Shi Yao Zhao | 2015 | Cell Research2015，25，3： | 16 |
| 17 | Mechanism of thalidomide to enhance cytotoxicity of temozolomide in U251-MG glioma cells in vitro显示文摘背景 Glioma 是有差的预后的最普通的主要大脑肿瘤。Temozolomide 与镇静药被使用了对待 gliomas。我们在人的恶意的 glioma 房间 U251-MG 是的 vitro.Methods 调查了这二药的 synergistic 机制对有为 3 天的不同处理的四个组有教养、分配:temozolomide 组(100 pmol/L ) ,镇静药组(100 pg/L ) ,加镇静药组(100 pg/L ) 的 temozolomide (100 IJmol/L ) 和控制组。MTT 试金被使用评估房间生存能力。房间周期被流动 cytometry 分析。autophagosomes 的极端结构的特征与电子显微镜被观察。氮蒽橘子和 monodansylcadavedne 被采用把 autophagosomes 标记,流动 cytometry 被申请 autophagosomes 的 quantification。联系 autophagy 的蛋白质的表示被肿瘤的西方的 blotting.Results 增长检测房间被 temozolomide 显然比由也药独自使用的与镇静药处理压制(P=-0.000 为每天) 。联合处理在极端结构的特性在联合处理以后被发现的 G0/G1 phase.Typical autophagic 导致了房间周期拘捕。镇静药支持了 temozolomide 导致的 autophagy。联系 autophagy 的蛋白质 - 微导管联系了蛋白质 1 轻链 3 (MAPILC3 ) 并且 Beclinl 比独自使用的 temozolomide 由联合处理是更显著地起来调整的(MAP1LC3, P=-0.000;Beclinl, P=-0.004 ) 。在染色体十上删除的磷酸酶和 tensin 相当或相同的事物(PTEN ) 的表示水平,由压制表明小径的 PI3K/Akt/mTOR 支持了 autophagy,被镇静药提高(镇静药组:P=-0.000;联合的组:P=0.002 ).Conclusions 镇静药由支持 temozolomide 导致的 autophagy 提高 temozolomide 的 cytotoxicity。由镇静药贡献 PTEN 的起来规定, autophagy 的表示联系了 protein-MAP1LC3, Beclinl 被提高,它在 vitro 在 temozolomide 和镇静药的联合处理导致增强的 autophagy。 | GAO Song YANG Xue-jun ZHANG Wen-gao JI Yan-wei PAN Qiang | 2009 | Chinese Medical Journal2009，，11： | 16 |
| 18 | A Cross-sectional Survey Assessing Carriage of Streptococcus pneumoniae in a Healthy Population in Xinjiang Uygur Autonomous Region of China显示文摘链球菌 pneumoniae 的车厢率和 serotype 分发(S。pneumoniae ) 在在中国遗体的一张健康人口不清楚。在这研究,我们在 Xinjiang 从 513 个个人收集了 oropharyngeal 拖把,中国。指向 lytA 基因和 12 serotypes 的实时 PCR 被估计识别 S。pneumoniae 马车。S 的全部的车厢率。pneumoniae 是 70.4%(361/513 ) 。最流行的 serotypes 是 19B/F, 18B/C, 5,并且 6A/B。S 的最高的车厢率。pneumoniae 6-10 年(88.6%) 在孩子被注意,它应得进一步的注意。二的合作殖民率或更多的 S。pneumoniae serotypes 是 79.8%(264/331 ) 。这研究试图在中国在健康个人之中调查基线 pneumococcal 马车率改进我们 S 的传染病学的理解。pneumoniae。 | XIE Na CHEN Zhao Yun CHEN Tao ZHU Bing Qing XU Li GAO Yuan ZHANG Ai Yu ZHAO Pan LIU Ji Wen SHAO Zhu Jun | 2018 | Biomedical and Environmental Sciences2018，31，3： | 15 |
| 19 | Synthesis, Crystal Structure and Theoretical Calculations of a New Co(Ⅱ) Coordination Polymer Based on 5-Nitroisophthalic Acid and Bis(imidazol) Ligands显示文摘A new complex[Co(NIPH)(mbix)]_n(1,H_2NIPH = 5-nitroisophthalic acid,mbix =l,3-bis(imidazol-l-ylmethyl)benzene) has been hydrothermally synthesized and structurally characterized by elemental analysis,IR spectrum,UV spectrum,TG and single-crystal X-ray diffraction.Pink crystals crystallize in the triclinic system,space group P1 with a = 8.3797(8),b= 10.2522(10),c= 13.4244(13) A,α=94.820(2),β=108.105(2),γ=104.816(2)°,V=1042.84(17) A^3,C_(22)H_(17)CoN_5O_6,M_r = 506.34,D_c = 1.613 g/cm^3,F(000) = 518,Z = 2,μ(MoKα) =0.876 mm^(-1),the final R = 0.0505 and wR = 0.1254 for 3267 observed reflections(I>2σ(I)).The structure of 1 exhibits a two-dimensional network structure and is extended into a three-dimensional supramolecule through hydrogen bonds and n-n interactions.In addition,Natural Bond Orbital(NBO) analysis was performed by using the PBE0/LANL2 DZ method built in Gaussian 09 Program.The calculation results showed obvious covalent interactions between the coordinated atoms and Co(Ⅱ) ion. | LI Xiu-Mei SUN Ming PAN Ya-Ru JI Jian-Ye | 2016 | Chinese Journal of Structural Chemistry2016，35，2： | 14 |
| 20 | Ginkgo biloba extract prevents against apoptosis induced by high glucose in human lens epithelial cells显示文摘瞄准:在人的透镜的高导致葡萄糖的 apoptosis 上调查白果树 biloba 摘录(家伙) 的保护的效果上皮的房间(HLEC ) 和可能的分子的机制。方法:有教养的 HLEC 被分派进 6 个组:正常的组,高葡萄糖组,低 -- ,中等 -- ,并且高剂量的 GBE 组,和 bendazac 离氨酸组。房间生存能力,房间 apoptosis,房间 antioxidases 的活动, aldose reductase, caspase-3,房间抗氧化剂的层次,和 Bcl-2 和 Bax 的表情被不同方法估计。结果:在为 24 h 与高葡萄糖被孵化以后, HLEC 经历了 apoptosis 并且展出了重要氧化应力。面对在不同剂量的 GBE, HLEC apoptosis 的率更低,氧化压力状态显著地被改善。到 Bcl-2 的 Bax 的增加的比率显著地被减少, caspase-3 的激活被 GBE 以一种剂量依赖者方式压制。结论: GBE 通过禁止氧化应力,把 Bax 的比率归结为 Bcl-2,并且减少阻止 HLEC 高导致葡萄糖的 apoptosis caspase-3 的活动。因此, GBE 对糖尿病的奔流形成有潜在的保护的效果。 | Zheng-mei WU Xiao-xing YIN Lei JI Yuan-yuan GAO Ying-mei PAN Qian LU Jian-yun WANG | 2008 | Acta Pharmacologica Sinica2008，29，9： | 12 |
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