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| 1 | Highly efficient,genotype-independent transformation and gene editing in watermelon(Citrullus lanatus)using a chimeric ClGRF4-GIF1 gene显示文摘Efficient genetic transformation has the potential to advance research and breeding in watermelon(Citrullus lanatus),but regeneration from tissue culture remains challenging.Previous work showed that expressing a fusion of two interacting transcription factors,GROWTH-REGULATING FACTOR4(GRF4)and GRF-INTERACTING FACTOR1(GIF1),improved regeneration in wheat(Triticum aestivum).By overexpressing a chimeric fusion of Cl GRF4 and Cl GIF1,we achieved highly efficient transformation in watermelon.Mutating the mi396 micro RNA target site in Cl GRF further boosted the transformation efficiency up to 67.27%in a genotype-independent manner.Cl GRF4-GIF1 can also be combined with clustered regularly interspaced palindromic repeats(CRISPR)/CRISPR-associated protein 9(Cas9)genome editing tools to achieve highly efficient gene editing in watermelon,which we used to successfully create diploid seedless watermelon.This research thus puts forward a powerful transformation tool for future watermelon research and breeding. | Qin Feng Ling Xiao Yizhen He Man Liu Jiafa Wang Shujuan Tian Xian Zhang Li Yuan | 2021 | Journal of Integrative Plant Biology2021，63，12： | 8 |
| 2 | Dissection of heterosis for yield and related traits using populations derived from introgression lines in rice显示文摘Despite the great success achieved by the exploitation of heterosis in rice,the genetic basis of heterosis is still not well understood.We adopted an advanced-backcross breeding strategy to dissect the genetic basis of heterosis for yield and eight related traits.Four testcross(TC) populations with 228 testcross F1 combinations were developed by crossing57 introgression lines with four types of widely used male sterile lines using a North Carolina II mating design.Analysis of variance indicated that the effects of testcross F1 combinations and their parents were significant or highly significant for most of the traits in both years,and all interaction effects with year were significant for most of the traits.Positive midparent heterosis(HMP) was observed for most traits in the four TC populations in the two years.The relative HMPlevels for most traits varied from highly negative to highly positive.Sixty-two dominant-effect QTL were identified for HMPof the nine traits in the four TC populations in the two years.Of these,22 QTL were also identified for the performance of testcross F1.Most dominant-effect QTL could individually explain more than 10% of the phenotypic variation.Four QTL clusters were observed including the region surrounding the RM9–RM297 region on chromosome 1,the RM110–RM279–RM8–RM5699–RM452 region on chromosome 2,the RM5463 locus on chromosome 6 and the RM1146–RM147 region on chromosome 10.The identified QTL for heterosis provide valuable information for dissecting the genetic basis of heterosis. | Chao Xiang Hongjun Zhang Hui Wang Shaobo Wei Binying Fu Jiafa Xia Zefu Li Yongming Gao Guoyou Ye | 2016 | The Crop Journal2016，4，6： | 3 |
| 3 | MicroRNAs Are Involved in Maize Immunity Against Fusarium verticillioides Ear Rot显示文摘Fusarium ear rot(FER)caused by Fusarium verticillioides is one of the most common diseases affecting maize production worldwide.FER results in severe yield losses and grain contamination with health-threatening mycotoxins.Although most studies to date have focused on comprehensive analysis of gene regulation in maize during defense responses against F.verticillioides infection,less is known about the role of micro RNAs(mi RNAs)in this process.We used deep sequencing to compare small RNA libraries from the maize kernels of susceptible(N6)or resistant(BT-1)inbred lines from uninfected plants and upon F.verticillioides infection.We found that pathogen exposure was accompanied by dynamic alterations in expression levels of multiple mi RNAs,including new members of previously annotated mi RNA families.A combination of transcriptomic,degradomic,and bioinformatics analyses revealed that F.verticillioides-responsive mi RNAs and their potential target genes displayed opposite expression patterns in the susceptible and resistant genotypes.Functional category analysis uncovered preferential enrichment of the pathogen-responsive mi RNAs and their targets in the phenylpropanoid metabolic processes,plant–pathogen interactions,and plant phytohormone signal transduction pathways.Furthermore,transgenic maize plants overexpressing mi R408 b exhibited reduced resistance to F.verticillioides infection in a susceptible maize line.These findings provide new insights into the regulatory roles of mi RNAs in maize immunity against FER and new resources for breeding disease resistance into maize. | Zijian Zhou Yan Cao Tao Li Xinghao Wang Jiafa Chen Hang He Wen Yao Jianyu Wu Huiyong Zhang | 2020 | Genomics, Proteomics & Bioinformatics2020，18，3： | 3 |
| 4 | Simultaneous improvement and genetic dissection of grain yield and its related traits in a backbone parent of hybrid rice ( Oryza sativa L.) using selective introgression显示文摘 | Hongjun Zhang Hui Wang Yiliang Qian Jiafa Xia Zefu Li Yingyao Shi Linghua Zhu Jauhar Ali Yongming Gao Zhikang Li | 2013 | Molecular Breeding2013，，1： | 1 |
| 5 | Energysaving direct ethanol production from viscosity reduction mash of sweet potato at very high gravity显示文摘 | Liang Zhang Qian Chen YanlingJin HuilinXue Jiafa Guan Zhongyan Wang Hai Zhao | 2010 | Fuel Processing Technology2010，，： | 1 |
| 6 | Effects of Soil Improver on Wheat in Saline-Alkali Lands in the Yellow River Delta显示文摘Field experiment carried out to test the effects of soil improver on wheat yield and soil physical-chemical properties. The results indicated that soil improver could optimize soil aggregates structure, decrease soil bulk density, soil pH and soil salt content, increase soil organic matter and 1 000-grain weight, thereby enhancing wheat yield. With the increase of soil improver application amount, soil physical-chemical properties became better and wheat yield increased. However, there was no significant difference in the treatments with the application amounts of 3%, 4% and 5%. In addition, the treatment of reducing nitrogen showed no superiority in soil physical-chemical properties and wheat yield, indicating that sufficient nitrogen was essential for the growth of wheat. | Liang DONG Shenzhong TIAN Zeqiang SUN Xuejun WANG Zhaohui LIU Ruiqin LI Ye TIAN Deshui TAN Jiafa LUO | 2019 | Agricultural Biotechnology2019，8，2： | 0 |
| 7 | Enhancing maize's nitrogen-fixing potential through ZmSBT3, a gene suppressing mucilage secretion∞显示文摘Maize(Zea mays)requires substantial amounts of nitrogen,posing a challenge for its cultivation.Recent work discovered that some ancient Mexican maize landraces harbored diazotrophic bacteria in mucilage secreted by their aerial roots.To see if this trait is retained in modern maize,we conducted a field study of aerial root mucilage(ARM)in 258 inbred lines.We observed that ARM secretion is common in modern maize,but the amount significantly varies,and only a few lines have retained the nitrogen‐fixing traits found in ancient landraces.The mucilage of the high‐ARM inbred line HN5‐724 had high nitrogen‐fixing enzyme activity and abundant diazotrophic bacteria.Our genome‐wide association study identified 17 candidate genes associated with ARM across three environments.Knockouts of one candidate gene,the subtilase family gene ZmSBT3,confirmed that it negatively regulates ARM secretion.Notably,the ZmSBT3 knockout lines had increased biomass and total nitrogen accumulation under nitrogen‐free culture conditions.High ARM was associated with three ZmSBT3 haplotypes that were gradually lost during maize domestication,being retained in only a few modern inbred lines such as HN5‐724.In summary,our results identify ZmSBT3 as a potential tool for enhancing ARM,and thus nitrogen fixation,in maize. | Jingyang Gao Peijiang Feng Jingli Zhang Chaopei Dong Zhao Wang Mingxiang Chen Zhongliang Yu Bowen Zhao Xin Hou Huijuan Wang Zhaokun Wu Razia Sultana Jemim Haidong Yu Doudou Sun Pei Jing Jiafa Chen Weibin Song Xuecai Zhang Zijian Zhou Jianyu Wu | 2023 | Journal of Integrative Plant Biology2023，65，12： | 0 |
| 8 | Developing a highly efficient CGBE base editor in watermelon显示文摘Cytosine and adenosine base editors(CBEs and ABEs)are novel genome-editing tools that have been widely utilized in molecular breeding to precisely modify single-nucleotide polymorphisms(SNPs)critical for plant agronomic traits and species evolution.However,conventional BE editors are limited to achieve C-to-T and A-to-G substitutions,respectively.To enhance the applicability of base editing technology in watermelon,we developed an efficient CGBE editor(SCGBE2.0)by removing the uracil glycosylase inhibitor(UGI)unit from the commonly used hA3A-CBE and incorporating the uracil-DNA glycosylase(UNG)component.Seven specific guide RNAs(sgRNAs)targeting five watermelon genes were designed to assess the editing efficiency of SCGBE.The results obtained from stably transformed watermelon plants demonstrated that SCGBE2.0 could efficiently induce C-to-G mutations at positions C5–C9 in 43.2%transgenic plants(with a maximum base conversion efficiency of 46.1%)and C-to-A mutation at position C4 in 23.5%transgenic plants(with a maximum base conversion efficiency of 45.9%).These findings highlight the capability of our integrated SCGBE2.0 editor to achieve C-to-G/A mutations in a site-preferred manner,thus providing an efficient base editing tool for precise base modification and site-directed saturated mutagenesis in watermelon. | Dong Wang Yani Chen Tao Zhu Jie Wang Man Liu Shujuan Tian Jiafa Wang Li Yuan | 2023 | Horticulture Research2023，10，9： | 0 |
| 9 | Causes of the Abnormal Seepage Field in a Dam with Asphaltic Concrete Core显示文摘Asphaltic concrete core(ACC) dams are widely built in China. Many ACC dams perform well, but others have experienced significant leakage including the case in western China studied herein. A numerical model of saturated-unsaturated water flow was adapted to simulate the seepage through the dam. By comparing the normal and abnormal seepage fields under different conditions, the main causes for the actual abnormal seepage field were identified and attributed to a defect in the ACC and an unintended, low permeability layer(LPL) in the transition zone(TZ) and the downstream dam shell. These conclusions are consistent with the situation and performance of the dam. Inadequate ACC construction processes might have caused defects in the ACC. An abrupt change of the ACC thickness probably induced stress concentrations and caused the ACC to fail. Material sources for the TZ and dam shell were complex and varied from specifications, and soil gradation for the TZ was inadequately controlled. In particular, tests show that the permeability varies over large ranges in these two parts of the dam. An unexpected LPL probably exists in both areas, and extends continuously. | Jiafa Zhang Jinlong Wang Haodong Cui | 2016 | Journal of Earth Science2016，27，1： | 0 |
| 10 | Creation of a watermelon haploid inducer line via ClDMP3-mediated single fertilization of the central cell显示文摘The use of doubled haploids is one of the most efficient breeding methods in modern agriculture.Irradiation of pollen grains has been shown to induce haploids in cucurbit crops,possibly because it causes preferential fertilization of the central cell over the egg cell.Disruption of the DMP gene is known to induce single fertilization of the central cell,which can lead to the formation of haploids.In the present study,a detailed method of creating a watermelon haploid inducer line via ClDMP3 mutation is described.The cldmp3 mutant induced haploids in multiple watermelon genotypes at rates of up to 1.12%.These haploids were confirmed via fluorescent markers,flow cytometry,molecular markers,and immuno-staining.The haploid inducer created by this method has the potential to greatly advance watermelon breeding in the future. | Xiner Chen Yuxiu Li Gongli Ai Jinfan Chen Dalong Guo Zhonghou Zhu Xuejie Zhu Shujuan Tian Jiafa Wang Man Liu Li Yuan | 2023 | Horticulture Research2023，10，6： | 0 |
| 11 | Unmanned aerial vehicle based intelligent triage system in mass-casualty incidents using 5G and artificial intelligence显示文摘BACKGROUND:Rapid on-site triage is critical after mass-casualty incidents(MCIs)and other mass injury events.Unmanned aerial vehicles(UAVs)have been used in MCIs to search and rescue wounded individuals,but they mainly depend on the UAV operator’s experience.We used UAVs and artificial intelligence(AI)to provide a new technique for the triage of MCIs and more efficient solutions for emergency rescue.METHODS:This was a preliminary experimental study.We developed an intelligent triage system based on two AI algorithms,namely OpenPose and YOLO.Volunteers were recruited to simulate the MCI scene and triage,combined with UAV and Fifth Generation(5G)Mobile Communication Technology real-time transmission technique,to achieve triage in the simulated MCI scene.RESULTS:Seven postures were designed and recognized to achieve brief but meaningful triage in MCIs.Eight volunteers participated in the MCI simulation scenario.The results of simulation scenarios showed that the proposed method was feasible in tasks of triage for MCIs.CONCLUSION:The proposed technique may provide an alternative technique for the triage of MCIs and is an innovative method in emergency rescue. | Jiafa Lu Xin Wang Linghao Chen Xuedong Sun Rui Li Wanjing Zhong Yajing Fu Le Yang Weixiang Liu Wei Han | 2023 | World Journal of Emergency Medicine2023，14，4： | 0 |
| 12 | A 4H-SiC semi-super-junction shielded trench MOSFET: p-pillar is grounded to optimize the electric field characteristics显示文摘A 4H-SiC trench gate metal-oxide-semiconductor field-effect transistor(UMOSFET)with semi-super-junction shiel-ded structure(SS-UMOS)is proposed and compared with conventional trench MOSFET(CT-UMOS)in this work.The advantage of the proposed structure is given by comprehensive study of the mechanism of the local semi-super-junction structure at the bottom of the trench MOSFET.In particular,the influence of the bias condition of the p-pillar at the bottom of the trench on the static and dynamic performances of the device is compared and revealed.The on-resistance of SS-UMOS with grounded(G)and ungrounded(NG)p-pillar is reduced by 52%(G)and 71%(NG)compared to CT-UMOS,respectively.Additionally,gate ox-ide in the GSS-UMOS is fully protected by the p-shield layer as well as semi-super-junction structure under the trench and p-base regions.Thus,a reduced electric-field of 2 MV/cm can be achieved at the corner of the p-shield layer.However,the quasi-intrinsic protective layer cannot be formed in NGSS-UMOS due to the charge storage effect in the floating p-pillar,resulting in a large electric field of 2.7 MV/cm at the gate oxide layer.Moreover,the total switching loss of GSS-UMOS is 1.95 mJ/cm2 and is reduced by 18%compared with CT-UMOS.On the contrary,the NGSS-UMOS has the slowest overall switching speed due to the weakened shielding effect of the p-pillar and the largest gate-to-drain capacitance among the three.The proposed GSS-UMOS plays an important role in high-voltage and high-frequency applications,and will provide a valuable idea for device design and circuit applications. | Xiaojie Wang Zhanwei Shen Guoliang Zhang Yuyang Miao Tiange Li Xiaogang Zhu Jiafa Cai Rongdun Hong Xiaping Chen Dingqu Lin Shaoxiong Wu Yuning Zhang Deyi Fu Zhengyun Wu Feng Zhang | 2022 | Journal of Semiconductors2022，43，12： | 0 |
| 13 | Genome-wide analysis of nuclear factor Y genes and functional investigation of watermelon ClNF-YB9 during seed development显示文摘The nuclear factor Y(NF-Y) gene family is a class of transcription factors that are widely distributed in eukaryotes and are involved in various biological processes. However, the NF-Y gene family members in watermelon, a valued and nutritious fruit, remain largely unknown and their functions have not been characterized. In the present study, 22 ClNF-Y genes in watermelon, 29 CsNF-Y genes in cucumber, and 24CmNF-Y genes in melon were identified based on the whole-genome investigation and their protein properties, gene location, gene structure, motif composition, conserved domain, and evolutionary relationship were investigated. ClNF-YB9 from watermelon and its homologs in cucumber and melon were expressed specifically in seeds. Its expression remained low in the early stages of watermelon seed development,increased at 20 days after pollination(DAP), and peaked at 45–50 DAP. Moreover, the knockout mutant Clnf-yb9 exhibited abnormal leafy cotyledon phenotype, implying its critical role during seed formation.Finally, protein interaction assays showed that ClNF-YB9 interacts with all ClNF-YCs and the ClNF-YB9-YC4 heterodimer was able to recruit a ClNF-YA7 subunit to assemble a complete NF-Y complex, which may function in seed development. This study revealed the structure and evolutionary relationships of the NF-Y gene family in Cucurbitaceae and the novel function of ClNF-YB9 in regulating seed development in watermelon. | Qin Feng Ling Xiao Jiafa Wang Jie Wang Chenyang Chen Jianyang Sun Xixi Wu Man Liu Xian Zhang Shujuan Tian Li Yuan | 2023 | The Crop Journal2023，11，5： | 0 |
| 14 | Genetic variation in ZmSO contributes to ABA response and drought tolerance in maize seedlings显示文摘Water scarcity impairs maize growth and yield.Identification and deployment of superior droughttolerance alleles is desirable for the genetic improvement of stress tolerance in maize.Our previous study revealed that maize sulfite oxidase(SO) catalyzes the oxidation of sulfite to sulfate and may be involved in drought response.But it was unclear whether the natural variation in Zm SO is directly associated with the drought resistance of maize.In the present study,we showed that Zm SO was associated with drought tolerance in maize seedlings,using gene association analysis and a transgene approach.A 14-bp insertion variation,containing two ABA-responsive elements,in the promoter region of Zm SO conferred ABAinducible expression,leading to increased drought tolerance.Genetic selection of this favorable allele increased drought tolerance.This study has identified elite alleles associated with sulfur metabolism for improving maize drought resistance. | Zongliang Xia Fangfang Liu Meiping Wang Jiafa Chen Zijian Zhou Jianyu Wu | 2023 | The Crop Journal2023，11，4： | 0 |
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