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| 1 | Parameters optimum matching of pure electric vehicle dual-mode coupling drive system显示文摘As an important development direction of pure electric vehicle drive system,the distributed drive system has the advantages of compact structure,high transmission efficiency,and flexible control,but there are some serious problems such as high performance requirements to the drive motors,complex control strategies,and poor reliability.To solve these problems,a two motors dual-mode coupling drive system has been developed at first,which not only has the capacity of two-speed gear shifting,but also can automatically switch between the distributed drive and the centralized drive by means of modes change control.So,the performance requirements to the drive motors can be reduced,the problem of abnormal running caused by the fault of unilateral distributed drive systems also can be resolved by replacing the drive mode with centralized drive.Then,the system parameters primary and the optimum matching under the principle of efficiency optimization have been carried out,which makes the drive system achieve predetermined functions and meet the actual demands of different operating statuses.At last,the economic comparison of a pure electric vehicle installation with a dual-mode coupling drive sytem,a single-motor centralized drive system or a dual-motor distributed drive system in the simulation conditions has been completed.Compared with other systems,the driving range of the electric vehicle driven by the designed system is significantly increased,which proves the better efficiency and application value of the system. | ZHANG LiPeng LI Liang QI BingNan WANG Chong ZHANG YaHui SONG Jian | 2014 | Science China(Technological Sciences)2014，57，11： | 22 |
| 2 | Expression of insulin-like growth factor Ⅱ and its receptor in liver cells of chronic liver diseases显示文摘ExpressionofinsulinlikegrowthfactorⅡanditsreceptorinlivercelsofchronicliverdiseasesYANGDongHua1,XIUChong1,YANGBo1,GUJianR... | YANG Dong Hua 1, XIU Chong 1, YANG Bo 1, GU Jian Ren 2, QIAN Lian Fang 2 and QU Shu Ming 2 | 1997 | World Journal of Gastroenterology1997，3，2： | 21 |
| 3 | Diverse modes of clonal evolution in HBV-related hepatocellular carcinoma revealed by single-cell genome sequencing显示文摘Hepatocellular 癌(HCC ) 是癌症实质词法,基因并且 phenotypic 差异。然而,我们不理解在 intratumor 异质和肿瘤的联系词法 / 组织学的特征之间的关系。用介绍 96 个肿瘤房间(30-36 各个) 和 15 个正常的肝房间(5 各个) 的单个房间的整个染色体的定序,与联系 HBV 的 HCC 从三个男病人收集了,我们证实拷贝数字变化在 hepatocarcinogenesis 早发生,但是此后在整个肿瘤仍然保持相对稳定前进。重要地,我们证明特定的 HCC 能具有 monoclonal 或 polyclonal 起源。有汇合的 multinodular 形态学的肿瘤是典型 polyclonal 肿瘤并且显示最高的 intratumor 异质。除了 mutational 和拷贝数字侧面,我们把了用导出 HBV 的外国 genomic 标记的 HCC 的同种细胞的起源。在 monoclonal HCC,所有肿瘤单身者房间展出一样的 HBV 集成,显示 HBV 集成是一个早司机事件并且在肿瘤前进期间仍然保持极其稳定。另外,我们的结果显示那两个都转移,迟了的传播并且早播种当模特儿,在 HCC 有一个角色前进。尤其是,开始的克隆传播的早 intrahepatic 导致同步 multifocal 肿瘤的形成。同时,我们在 HCC 识别了潜在的司机基因 ZNF717,它展出变化的高频率在单个房间并且人口铺平,,通过调整 IL-6/STAT3 小径行动的肿瘤 suppressor。这些调查结果热点多重不同肿瘤在 HCC 的进化机制,它为特定的处理策略建议需要。 | Meng Duan, Shu Zhang, Zhichao Wang, Jieyi Shi, Longzi Liu, Xiaoying Wang, Aiwu Ke, Jian Zhou, Jia Fan, Qiang Gao Junfeng Hao, Chong Li Sijia Cui Daniel L Worthley Ya Cao Ruibin Xi Xiaoming Zhang Jian Zhou, Jia Fan Qiang Gao | 2018 | Cell Research2018，28，3： | 21 |
| 4 | Imaging in detecting sites of pulmonary fibrosis induced by paraquat显示文摘 | Xiao-li Xu Wei Wang Zu-jun Song Hong Ding Xiao-hong Duan Huan-cheng Meng Jian Chong | 2011 | World Journal of Emergency Medicine2011，2，1： | 19 |
| 5 | Effects of residual organics in municipal wastewater on hydrogenotrophic denitrifying microbial communities显示文摘Hydrogenotrophic denitrification is promising for tertiary nitrogen removal from municipal wastewater. To reveal the influence of residual organics in municipal wastewater on hydrogenotrophic denitrifiers, we adopted high-throughput 16 S r RNA gene amplicon sequencing to examine microbial communities in hydrogenotrophic denitrification enrichments. Using effluent from a municipal wastewater treatment plant as water source, COD,nitrate and p H were controlled the same except for a gradient of biodegradable carbon(i.e., primary effluent(PE), secondary effluent(SE), or combined primary and secondary effluent(CE)). Inorganic synthetic water(IW) was used as a control. Hydrogenophaga, a major facultative autotroph, accounted for 17.1%, 5.3%, 32.7% and 12.9% of the sequences in PE, CE,SE and IW, respectively, implicating that Hydrogenophaga grew well with or without organics.Thauera, which contains likely obligate autotrophic denitrifiers, appeared to be the most dominant genera(23.6%) in IW and accounted for 2.5%, 4.6% and 8.9% in PE, CE and SE,respectively. Thermomonas, which is related to heterotrophic denitrification, accounted for 4.2% and 7.9% in PE and CE fed with a higher content of labile organics, respectively.In contrast, Thermomonas was not detected in IW and accounted for only 0.6% in SE. Our results suggest that Thermomonas are more competitive than Thauera in hydrogenotrophic denitrification with biodegradable organics. Moreover, facultative autotrophic denitrifiers,Hydrogenophaga, are accommodating to residual organic in effluent wastewater, thus we propose that hydrogenotrophic denitrification is amenable for tertiary nitrogen removal. | Wei Xing Jinlong Li Peng Li Chong Wang Yanan Cao Desheng Li Yunfeng Yang Jizhong Zhou Jiane Zuo | 2018 | Journal of Environmental Sciences2018，30，3： | 16 |
| 6 | Involvement of sphingoid bases in mediating reactive oxygen intermediate production and programmed cell death in Arabidopsis显示文摘Sphingolipids 被建议了在植物房间为一连串的细胞的发信号活动充当第二个送信人,包括压力回答并且规划了房间死亡(PCD ) 。然而,这些过程不是的机制 underpinning 很好理解。这里,我们报导那 Arabidopsis 异种, fumonisin B1 抵抗 11-1 ( fbr 11-1 )没能产生反应的氧中介( ROI ),不能当异种被 fumonisin B ( 1 )( FB ( 1 ))质问时,开始 PCD , ceramide synthase 的一个特定的禁止者。显示的分子的分析 FBR11 编码一个长链的底 1 (LCB1 ) 丝氨酸 palmitoyltransferase (SPT ) 的子单元,它催化 de novo sphingolipid 的第一限制率的步合成。sphingolipid 集中的集体度谱的分析表明而 fbr 11-1 变化没影响 sphingoid 底的基础层次,异种响应 FB (1 ) 显示出 sphingoid 底的稀释形成。由一个直接的喂实验,我们证明免费 sphingoid 底 dihydrosphingosine, phytosphingosine 和鞘氨醇高效地导致产生由房间死亡跟随了的 ROI。相反地, dihydrosphingosine 导致的 ROI 产生和房间死亡被它的 phosphorylated 形式 dihydrosphingosine-1-phosphate 明确地以一种剂量依赖者方式堵住,建议在一个免费 sphingoid 底和它的 phosphorylated 衍生物之间的动态平衡的维护对决定房间命运批评。因为 sphingolipid 水平的改变发生在 ROI 生产以前,我们建议免费 sphingoid 库在 Arabidopsis 涉及 PCD 的控制,大概在收到不同发展或环境的暗示之上通过 ROI 的规定铺平。 | Lihua Shi Jacek Bielawski Jinye Mu Haili Dong Chong Teng Jian Zhang Xiaohui Yang Nario Tomishige Kentaro Hanada Yusuf A Hannun Jianru Zuo | 2007 | Cell Research2007，17，12： | 12 |
| 7 | Detailed Inventory Mapping and Spatial Analyses to Landslides Induced by the 2013 Ms 6.6 Minxian Earthquake of China显示文摘On July 22, 2013, an earthquake(Ms 6.6) occurred in Minxian, Gansu Province of China, causing a large number of landslides. Based on high resolution remote sensing images before and after this event, we made the visual interpretation to these coseismic landslides, and prepared a detailed inventory.The inventory registers totally 6 478 landslides in the study area. Of them, 3 322 landslides are larger than 100 m^2. Based on 5 m resolution DEM, these landslides were used to perform spatial analyses using landslide number density(LND) and landslide area percentage(LAP). The results show that the highest LND and LAP values are in the elevation range of 2 300–2 500 m and steeper slopes. Slopes facing E, SE, S and SW directions, slopes with larger absolute curvature values, ridges, scopes of gravel beds of Late Pleistocene(Qp) and the VIII-degree seismic intensity are more prone to sliding with high LND and LAP values. The largest LND and LAP values are in the scopes of 0.08 and 0.24 g, respectively. According to landslide distribution, we infer that F2-2 branch of Lintan-Dangchang fault is the seismogenic fault. With the increasing distances to this branch fault and drainages, LND and LAP values tend to decrease. | Yingying Tian Chong Xu Xiwei Xu Jian Chen | 2016 | Journal of Earth Science2016，27，6： | 10 |
| 8 | Outcomes in children with biliary atresia following liver transplantation显示文摘BACKGROUND: Congenital biliary atresia is a rare condition characterized by idiopathic dysgenesis of the bile ducts. If untreated, congenital biliary atresia leads to liver cirrhosis, liver failure and premature death. The present study aimed to evaluate the outcomes of orthotopic liver transplantation in children with biliary atresia. METHOD: We retrospectively analyzed 45 patients with biliary atresia who had undergone orthotopic liver transplantation from September 2006 to August 2012. RESULTS: The median age of the patients was 11.0 months (5-102). Of the 45 patients, 41 were younger than 3 years old. Their median weight was 9.0 kg (4.5-29.0), 34 of the 45 patients were less than 10 kg. Thirty-one patients had undergone Kasai portoenterostomy prior to orthotopic liver transplantation. We performed 30 living donor liver transplants and 15 split liver transplants. Six patients died during a follow-up. The median follow-up time of surviving patients was 11.4 months (1.4-73.7). The overall 1-, 2- and 3-year survival rates were 88.9%, 84.4% and 84.4%, respectively. CONCLUSION: With advances in surgical techniques and management, children with biliary atresia after liver transplantation can achieve satisfactory survival in China, although there remains a high risk of complications in the early postoperative period. | Li-Ying Sun Yun-Sheng Yang Zhi-Jun Zhu Wei Gao Lin Wei Xiao-Ye Sun Wei Qu Wei Rao Zhi-Gui Zeng Chong Dong Jin-Peng Tu Jian Wang Yi-He Liu Yuan Liu Li-Xin Yu Yu Wang Jing Li Zhong-Yang Shen | 2013 | Hepatobiliary & Pancreatic Diseases International2013，12，2： | 8 |
| 9 | Status and development of high-power laser facilities at the NLHPLP显示文摘In this paper, we review the status of the multifunctional experimental platform at the National Laboratory of High Power Laser and Physics(NLHPLP). The platform, including the SG-II laser facility, SG-II 9th beam, SG-II upgrade(SG-II UP) facility, and SG-II 5 PW facility, is operational and available for interested scientists studying inertial confinement fusion(ICF) and a broad range of high-energy-density physics. These facilities can provide important experimental capabilities by combining different pulse widths of nanosecond, picosecond, and femtosecond scales. In addition, the SG-II UP facility, consisting of a single petawatt system and an eight-beam nanosecond system, is introduced including several laser technologies that have been developed to ensure the performance of the facility. Recent developments of the SG-II 5 PW facility are also presented. | Jianqiang Zhu Jian Zhu Xuechun Li Baoqiang Zhu Weixin Ma Xingqiang Lu Wei Fan Zhigang Liu Shenlei Zhou Guang Xu Guowen Zhang Xinglong Xie Lin Yang Jiangfeng Wang Xiaoping Ouyang Li Wang Dawei Li Pengqian Yang Quantang Fan Mingying Sun Chong Liu Dean Liu Yanli Zhang Hua Tao Meizhi Sun Ping Zhu Bingyan Wang Zhaoyang Jiao Lei Ren Daizhong Liu Xiang Jiao Hongbiao Huang Zunqi Lin | 2018 | High Power Laser Science and Engineering2018，6，4： | 8 |
| 10 | Prediction of 10-year Atherosclerotic Cardiovascular Disease Risk among Adults Aged 40-79 Years in China:a Nationally Representative Survey显示文摘Objective To establish the distribution of 10-year atherosclerotic cardiovascular disease(ASCVD) risk among Chinese adults.Methods We estimated the 10-year ASCVD risk by applying the 2013 American College of Cardiology/American Heart Association pooled cohort equations(PCEs) to the data obtained from the 2010 China Chronic Disease and Risk Factor Surveillance that involved 61,541 participants(representing 520,158,652 Chinese adults) aged 40-79 years.We also compared the ASCVD risk with the 10-year ischemic cardiovascular disease(ICVD) risk,which was calculated using the simplified scoring tables recommended by the Chinese Guidelines for Prevention of Cardiovascular Diseases(Chinese model).Results Based on the PCEs,the average 10-year ASCVD risk among adults without self-reported stroke or myocardial infraction was 12.5%.Approximately 247 million(47.4%) and 107 million(20.6%) adults had ≥ 7.5% and > 20% 10-year ASCVD risks,respectively.The 10-year ASCVD risk > 20% was higher among men,less educated individuals,smokers,drinkers,and physically inactive individuals than among their counterparts.Overall,29.0% of adults categorized using the Chinese model were overclassified with the PCEs.Conclusion Our results define the distribution of 10-year ASCVD risk among Chinese adults.The 10-year ASCVD risk predicted by the PCEs was higher than the ICVD risk predicted by the Chinese model. | ZHANG Mei JIANG Yong WANG Li Min LI Yi Chong HUANG Zheng Jing LI Jian Hong ZHOU Mai Geng ZHAO Wen Hua 无 | 2017 | Biomedical and Environmental Sciences2017，30，4： | 7 |
| 11 | Development of a Rapid Multi-residue Assay for Detecting β-lactams Using Penicillin Binding Protein 2x显示文摘Objective To develop a rapid multi-residue assay for detecting 16 demanded by the European Union (EU). Methods A recombinant penicillin-binding protein(PBP)2x* from Streptococcus pneumoniae R6 was expressed in vitro and six β-lactams were conjugated to HRP by four methods.A rapid multi-residue assay for β-lactams was established with PBP2x* and HRP-conjugate. Results PBP2x* was expressed and purified successfully and the ideal HRP-conjugate was identified. The multi-residue assay was developed.After optimization,penicillin G,ampicillin,amoxicillin, cloxacillin,dicloxacillin,oxacillin,nafcillin,cephalexin,ceftiofur,cefaIonium,cefquinome,cefazolin, cefoperazone,cephacetrile,and cephapirin can be detected at levels below MRL in milk with simple pretreatment. Conclusion This assay developed can detect all 16 β-lactams demanded by the European Union(EU). The whole procedure takes only 45 min and can detect 42 samples and the standards with duplicate analysis. | ZENG Kum ZHANG Jing WANG Yang WANG ZhanHui ZHANG Su Xia WU Chong Ming SHEN Jian Zhong | 2013 | Biomedical and Environmental Sciences2013，26，2： | 7 |
| 12 | Importance of identifying tropical cyclone tornadoes in typhoon warning and defense systems显示文摘Tornadoes are highly localized severe weather phenomena and have been of increasing concern in China [1] in recent years, especially after the sinking of the Oriental Star in 2015 [2] and severe tornado damage in Yancheng in 2016 (3)Tornadoes induced by tropical cyclones, however, have received little attention.A recent study has shown that a large number of tornado-scale vortices can be produced at the inner edge of the intense eyewallconvection of tropical cyclones [4]. | Dan Yao Xudong Liang Qing Meng Jian Li Chong Wu Zhengshuai Xie Dandan Chen Jianping Guo | 2019 | Science Bulletin2019，64，3： | 7 |
| 13 | Microbial community structures in an integrated two-phase anaerobic bioreactor fed by fruit vegetable wastes and wheat straw显示文摘The microbial community structures in an integrated two-phase anaerobic reactor(ITPAR)were investigated by 16S r DNA clone library technology.The 75 L reactor was designed with a 25 L rotating acidogenic unit at the top and a 50 L conventional upflow methanogenic unit at the bottom,with a recirculation connected to the two units.The reactor had been operated for 21 stages to co-digest fruit/vegetable wastes and wheat straw,which showed a very good biogas production and decomposition of cellulosic materials.The results showed that many kinds of cellulose and glycan decomposition bacteria related with Bacteroidales,Clostridiales and Syntrophobacterales were dominated in the reactor,with more bacteria community diversities in the acidogenic unit.The methanogens were mostly related with Methanosaeta,Methanosarcina,Methanoculleus,Methanospirillum and Methanobacterium;the predominating genus Methanosaeta,accounting for 40.5%,54.2%,73.6%and 78.7%in four samples from top to bottom,indicated a major methanogenesis pathway by acetoclastic methanogenesis in the methanogenic unit.The beta diversity indexes illustrated a more similar distribution of bacterial communities than that of methanogens between acidogenic unit and methanogenic unit.The differentiation of methanogenic community composition in two phases,as well as pH values and volatile fatty acid(VFA)concentrations confirmed the phase separation of the ITPAR.Overall,the results of this study demonstrated that the special designing of ITPAR maintained a sufficient number of methanogens,more diverse communities and stronger syntrophic associations among microorganisms,which made two phase anaerobic digestion of cellulosic materials more efficient. | Chong Wang Jiane Zuo Xiaojie Chen Wei Xing Linan Xing Peng Li Xiangyang Lu Chao Li | 2014 | Journal of Environmental Sciences2014，26，12： | 6 |
| 14 | MicroRNA-22 inhibits proliferation and promotes differentiation of satellite cells in porcine skeletal muscle显示文摘Pig is an important economic animal in China. Improving meat quality and meat productivity is a long time issue in animal genetic breeding. Micro RNAs(mi RNAs) are short non-coding RNAs that participate in various biological processes, such as muscle development and embryogenesis. mi R-22 differentially expresses in embryonic and adult skeletal muscle. However, the underlying mechanism is unclear. In this study, we investigated mi R-22 function in proliferation and differentiation of porcine satellite cells(PSCs) in skeletal muscle. Our data show that mi R-22 expressed in both proliferation and differentiated PSCs and is significantly upregulated(P<0.05) during differentiation. After treated with the mi R-22 inhibitor, PSCs proliferation was significantly increased(P<0.05), as indicated by the up-regulation(P<0.01) of cyclin D1(CCND1), cyclin B1(CCNB1) and down-regulation(P<0.05) of P21. Conversely, over-expression of mi R-22 resulted in opposite results. Differentiation of PSCs was significantly suppressed(P<0.05), evidenced by two major myogenic markers: myogenin(Myo G) and myosin heavy chain(My HC), after transfecting the PSCs with mi R-22 inhibitor. Opposite results were demonstrated in the other way around by transfection with mi R-22 mimics. In conclusion, the data from this study indicated that mi R-22 inhibited the PSCs proliferation but promoted their differentiation. | Hong Quyen Dang XU Gu-li HOU Lian-jie XU Jian HONG Guang-liang Chingyuan Hu WANG Chong | 2020 | Journal of Integrative Agriculture2020，19，1： | 5 |
| 15 | A transcription factor STOP1-centered pathway coordinates ammonium and phosphate acquisition in Arabidopsis显示文摘Phosphorus(P)is an indispensable macronutrient required for plant growth and development.Natural phosphate(Pi)reserves are finite,and a better understanding of Pi utilization by crops is therefore vital for worldwide food security.Ammonium has long been known to enhance Pi acquisition efficiency in agriculture;however,the molecular mechanisms coordinating Pi nutrition and ammonium remains unclear.Here,we reveal that ammonium is a novel initiator that stimulates the accumulation of a key regulatory protein,STOP1,in the nuclei of Arabidopsis root cells under Pi deficiency.We show that Pi deficiency promotes ammonium uptake mediated by AMT1 transporters and causes rapid acidification of the root surface.Rhizosphere acidification-triggered STOP1 accumulation activates the excretion of organic acids,which help to solubilize Pi from insoluble iron or calcium phosphates.Ammonium uptake by AMT1 transporters is downregulated by a CIPK23 protein kinase whose expression is directly modulated by STOP1 when ammonium reaches toxic levels.Taken together,we have identified a STOP1-centered regulatory network that links external ammonium with efficient Pi acquisition from insoluble phosphate sources.These findings provide a framework for developing possible strategies to improve crop production by enhancing the utilization of non-bioavailable nutrients in soil. | Wen Hao Tian Jia Yuan Ye Meng Qi Cui Jun Bo Chang Yu Liu GuiXin Li Yun Rong Wu Ji Ming Xu Nicholas P.Harberd Chuan Zao Mao Chong Wei Jin Zhong Jie Ding Shao Jian Zheng | 2021 | Molecular Plant2021，14，9： | 5 |
| 16 | 酶解乳蛋白对初生仔猪小肠组织学结构及隐窝细胞增殖的影响(英文)显示文摘INTRODUCTIONTheintestineasthemajorinterfacebetweenanorganismanditsnutritionalenvironmentplaysacriticalroleinpostnataldevelopm... | Tian Wang 1,Ruo Jun Xu 2, Yuna Qiang Zhang 3, Chong Tian Zheng 1 and Jian Cheng Zhang 4 1 Department of Animal Nutrition and Feed, Nanjing Agricultural University,210095 2 Department of Zoology, The University of Hong Kong 3 Department | 1999 | 中国组织化学与细胞化学杂志1999，8，2： | 5 |
| 17 | Prevalence of Major Cardiovascular Risk Factors and Cardiovascular Disease in Women in China: Surveillance Efforts显示文摘在这研究,我们试图与风险介绍的心血管的疾病(CVD ) 和 CVD 估计社会经济的地位和文化移入的关系并且检验与 CVD 联系的 CVD 风险因素。我们从 2010 中国长期的疾病和风险因素监视调查使用了数据,它由女人的一件全国地代表性的样品组成了。下列流行被发现:心肌的梗塞(MI ) :0.4% ;击:0.5% ;反常 cholesterolemia:44.9% ;过重或肥胖:32.2% ;高血压:31.7% ;糖尿病:9.0% ;并且吸烟:2.5% 。总共, 30.9% 中国女人没有风险因素,但是 13.3% 有的 ≥3 联系了风险因素。在调整 multivariate 的模型,高血压,糖尿病,超重或肥胖,并且吸烟都直接与 MI 被联系;为中风,协会与高血压,反常 cholesterolemia,糖尿病,和过重或肥胖是积极的。因此, CVD 风险因素在 ≥18 年的中国女人之中是普通的,这能被结束。 | LI Jian Hong WANG Li Min LI Yi Chong ZHANG Mei WANG Lin Hong | 2016 | Biomedical and Environmental Sciences2016，29，3： | 4 |
| 18 | Microstructural characteristics and mechanical properties of bobbin-tool friction stir welded 2024–T3 aluminum alloy显示文摘Cold-rolled 2024–T3 sheet alloy was subjected to bobbin-tool friction stir welding(BTFSW). The microstructural characteristics and mechanical properties of the nugget zone in the as-welded state were investigated. The results show that the equiaxed grain size of BTFSW 2024–T3 alloy decreases from 7.6 to 2.8 μm as the welding speed is increased from 80 to 120 mm/min; in addition,fine grains are generated in the nugget zone and the size distribution is non-uniform. All Al_2 Cu Mg(S′) precipitates dissolve into the Al matrix,whereas Mn-rich phases confirmed as T phases(Al_(20)Cu_2Mn_3,Al_6 Mn,or Al_3Mn) remain unchanged. The optimized parameters for BTFSW are verified as the rotation speed of 350 r/min and the travel speed of 100 mm/min. The variations in precipitation and dislocation play more important roles than grain size in the nugget zone with respect to influencing the mechanical properties during the BTFSW process. After the BTFSW process,the fracture mode of base material 2024–T3 alloy transforms from ductile rupture to ductile-brittle mixed fracture. | Ji-hong Dong Chong Gao Yao Lu Jian Han Xiang-dong Jiao Zhi-xiong Zhu | 2017 | International Journal of Minerals,Metallurgy and Materials2017，24，2： | 4 |
| 19 | Separation and enrichment of trace ractopamine in biological samples by uniformly-sized molecularly imprinted polymers显示文摘In order to prepare a high capacity packing material for solid-phase extraction with specific recognition ability of trace ractopamine in biological samples, uniformly-sized, molecularly imprinted polymers (MIPs) were prepared by a multi-step swelling and polymerization method using methacrylic acid as a functional monomer, ethylene glycol dimethacrylate as a cross-linker, and toluene as a porogen respectively. Scanning electron microscope and specific surface area were employed to identify the characteristics of MIPs. Ultraviolet spectroscopy, Fourier transform infrared spectroscopy, Scatchard analysis and kinetic study were performed to interpret the specific recognition ability and the binding process of MIPs. The results showed that, compared with other reports, MIPs synthetized in this study showed high adsorption capacity besides specific recognition ability. The adsorption capacity of MIPs was 0.063 mmol/g at 1 mmol/L ractopamine concentration with the distribution coefficient 1.70. The resulting MIPs could be used as solid-phase extraction materials for separation and enrichment of trace ractopamine in biological samples. | Ya Li Qiang Fu Meng Liu Yuan-Yuan Jiao Wei Du Chong Yu Jing Liu Chun Chang Jian Lu | 2012 | Journal of Pharmaceutical Analysis2012，2，6： | 4 |
| 20 | Construction of a chloroplast protein interaction network and functional mining of photosynthetic proteins in Arabidopsis thaliana显示文摘叶绿体是光合作用发生的一个典型植物细胞细胞器。在这研究,在 Arabidopsis thaliana 的 1 808 叶绿体核心蛋白质的一个总数被联合以前出版的研究和我们的自己的预言的结果可靠地识别。我们然后首先基于这些核心蛋白质相互作用构造了一个叶绿体蛋白质相互作用网络。网络有 22 包含了 2 的 925 蛋白质相互作用对 214 蛋白质。160 的一个总数以前, uncharacterized 蛋白质在这个网络被注解。光合的建筑群的子单元被模块化,并且在 photosystem 之中的功能的关系我(希腊语的第二十三个字母), photosystem II ( PSII ), photosystem 的轻收获建筑群我( LHC 我)并且 photosystem 的轻收获建筑群( LHC II )我能在这个网络从预言的蛋白质相互作用被推出。我们进一步由酵母证实了在未知蛋白质 AT1G52220 和光合的子单元 PSI-D2 之间的一个相互作用二混血儿的分析。我们的叶绿体蛋白质相互作用网络应该为光合的蛋白质的功能的采矿和在在 Arabidopsis 的系统生物学水平的叶绿体相关的函数的调查是有用的。 | Qing-Bo Yu Guang Li Guan Wang Jing-Chun Sun Peng-Cheng Wang Chen Wang Hua-Ling Mi Wei-Min Ma Jian Cui Yong-Lan Cui Kang Chong Yi-Xue Li Yu-Hua Li Zhongming Zhao Tie-LiuShi Zhong-Nan Yang | 2008 | Cell Research2008，18，10： | 4 |
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