|
|
|
题名
|
作者
|
年代
|
出处
|
被引量
|
| 1 | DEVELOPMENT OF STEP-POOL SEQUENCE AND ITS EFFECTS IN RESISTANCE AND STREAM BED STABILITY显示文摘Experiments were conducted and field investigations were performed to study the development of step-pool sequence and its effects on resistance to the flow and stream bed stability. Step-pool sequence develops in incised channels as a result of streambed erosion, which is compared with sand dunes and armor layer of the role in resistance and streambed protection. The tight interlocking of particles in steps gives them an inherent stability which only extreme floods are likely to disturb. That stability suggests that step-pools are a valid equilibrium form, especially when coupled with their apparent regularity form and their role in satisfying the extreme condition of resistance maximization. The development degree of step-pools, SP, is proportional to the streambed slope. If the incoming sediment load is equal to or more than the sediment-carrying capacity of the flow, there is no bed erosion and thence there are no step-pools. Ifthe flow depth increases and is over the step-height the resistance caused by the step-pool sequence will be greatly reduced. The rate of energy dissipation by step-pools is a function of SP. The higher is SP, the larger is the rate of energy dissipation. The step-pool sequence increases the resistance and flow depth, reduces the shear stress of the flow and protects the streambed from erosion. Moreover,step-pool sequence provides ecologically sound habitats for aquatic bio-community as well. | Zhao-YinWANG JiangXU ChangzhiLI | 2004 | International Journal of Sediment Research2004，19，3： | 18 |
| 2 | MicroRNA in vivo precipitation identifies miR-151-3p as a computational unpredictable miRNA to target Stat3 and inhibits innate IL-6 production显示文摘MicroRNAs(miRNAs)function as important regulators in the immune response and inflammation.Several approaches have been reported to computationally predict miRNAs and their potential targets.However,there are still many miRNA–target interactions that are unpredictable by using the current computational algorithms.We established a miRNA in vivo precipitation method(miRIP)to identify unpredictable miRNAs with definite targets in these cells.Because Stat3 is a well-known transcription factor involved in innate immunity and inflammation,we utilized the miRIP method to identify miRNAs that bind Stat3 mRNA in macrophages.Among the captured miRNAs,miR-151-3p was confirmed to interact with Stat3 mRNA 3′-UTR and downregulate the Stat3 protein levels.LPS stimulation decreased miR-151-3p expression,thereby increasing IL-6 production.Therefore,we found that miR-151-3p inhibited LPS-induced IL-6 production by targeting Stat3.These data further confirmed miRIP as an efficient method to identify unpredictable miRNAs and explore miRNAs-mediated regulation in innate immunity and inflammation. | Xiang Liu Xiaoping Su Sheng Xu Huamin Wang Dan Han Jiangxue Li Mingyan Huang Xuetao Cao | 2018 | Cellular & Molecular Immunology2018，15，2： | 8 |
| 3 | Investigation and experimental validation of curcumin-related mechanisms against hepatocellular carcinoma based on network pharmacology显示文摘Objective:To determine the potential molecular mechanisms underlying the therapeutic effect of curcumin on hepatocellular carcinoma(HCC)by network pharmacology and experimental in vitro validation.Methods:The predictive targets of curcumin or HCC were collected from several databases.the identified overlapping targets were crossed with Gene Ontology(GO)and Kyoto Encyclopedia of Genes and Genomes(KEGG)analyses using the Database for Annotation,Visualization,and Integrated Discovery(DAVID)platform.Two of the candidate pathways were selected to conduct an experimental verification.The 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide tetrazolium(MTT)assay was used to determine the effect of curcumin on the viability of Hep G2 and LO2 cells.The apoptosis and autophagy of Hep G2 cells were respectively detected by flow cytometry and transmission electron microscopy.Besides,western blot and real-time polymerase chain reaction(PCR)were employed to verify the p53 apoptotic pathway and adenosine 5’-monophosphate(AMP)-activated protein kinase(AMPK)autophagy pathway.Hep G2 cells were pretreated with pifithrin-α(PFT-α)and GSK690693 for further investigation.Results:The 167 pathways analyzed by KEGG included apoptosis,autophagy,p53,and AMPK pathways.The GO enrichment analysis demonstrated that curcumin was involved in cellular response to drug,regulation of apoptotic pathway,and so on.The in vitro experiments also confirmed that curcumin can inhibit the growth of Hep G2 cells by promoting the apoptosis of p53 pathway and autophagy through the AMPK pathway.Furthermore,the protein and messenger RNA(m RNA)of the two pathways were downregulated in the inhibitor-pretreated group compared with the experimental group.The damage-regulated autophagy modulator(DRAM)in the PFT-α-pretreated group was downregulated,and p62 in the GSK690693-pretreated group was upregulated.Conclusions:Curcumin can treat HCC through the p53 apoptotic pathway and the AMPK/Unc-51-like kinase 1(ULK1)autophagy pathway,in which the mutual transformation of autophagy and apoptosis may occur through DRAM and p62. | Yang CHEN Qian LI Sisi REN Ting CHEN Bingtao ZHAI Jiangxue CHENG Xiaoyan SHI Liang SONG Yu FAN Dongyan GUO | 2022 | Journal of Zhejiang University-Science B(Biomedicine & Biotechnology)2022，23，8： | 3 |
| 4 | Synthetic lethal short hairpin RNA screening reveals that ring finger protein 183 confers resistance to trametinib in colorectal cancer cells显示文摘Background: The mitogen-activated extracellular signal-regulated kinase 1/2(MEK1/2) inhibitor trametinib has shown promising therapeutic effects on melanoma, but its efficacy on colorectal cancer(CRC) is limited. Synthetic lethality arises with a combination of two or more separate gene mutations that causes cell death, whereas individual mutations keep cells alive. This study aimed to identify the genes responsible for resistance to trametinib in CRC cells,using a synthetic lethal short hairpin RNA(shRNA) screening approach.Methods: We infected HT29 cells with a pooled lentiviral shRNA library and applied next-generation sequencing to identify shRNAs with reduced abundance after 8-day treatment of 20 nmol/L trametinib. HCT116 and HT29 cells were used in validation studies. Stable ring finger protein 183(RNF183)-overexpressing cell lines were generated by pcDNA4-myc/his-RNF183 transfection. Stable RNF 183-knockdown cell lines were generated by infection of lentiviruses that express RNF183 shRNA, and small interference RNA(siRNA) was used to knock down RNF183 transiently.Quantitative real-time PCR was used to determine the mRNA expression. Western blotting, immunohistochemical analysis, and enzyme-linked immunosorbent assay(ELISA) were used to evaluate the protein abundance. MTT assay,colony formation assay, and subcutaneous xenograft tumor growth model were used to evaluate cell proliferation.Results: In the primary screening, we found that the abundance of RNF183 shRNA was markedly reduced after treatment with trametinib. Trametinib induced the expression of RNF183, which conferred resistance to drug-induced cell growth repression and apoptotic and non-apoptotic cell deaths. Moreover, interleukin-8(IL-8) was a downstream gene of RNF183 and was required for the function of RNF183 in facilitating cell growth. Additionally, elevated RNF183 expression partly reduced the inhibitory effect of trametinib on IL-8 expression. Finally, xenograft tumor model showed the synergism of RNF183 knockdown and trametinib in repressing the growth of CRC cells in vivo.Conclusion: The RNF183-IL-8 axis is responsible for the resistance of CRC cells to the MEK1/2 inhibitor trametinib and may serve as a candidate target for combined therapy for CRC. | Rong Geng Xin Tan Zhixiang Zuo Jiangxue Wu Zhizhong Pan Wei Shi Ranyi Liu Chen Yao Gaoyuan Wang Jiaxin Lin Lin Qiu Wenlin Huang Shuai Chen | 2017 | Chinese Journal of Cancer2017，36，12： | 2 |
| 5 | Acute toxicity and biodistribution of different sized titanium dioxide particles in mice after oral administration显示文摘 | Wang Jiangxue Zhou Guoqiang Chen Chunying | 2007 | Toxicology Letters2007，1681，： | 1 |
| 6 | Wheel Slip Measurement in 4WD Tractor Based on LABVIEW显示文摘 | Zhixiong Lu Yang Lu Jiangxue Chang Xuefeng Bai Yiguan Liu | 2013 | International Journal of Automation and Control Engineering2013，，3： | 1 |
| 7 | Genetic and Epigenetic Down-regulation of MicroRNA-212 Promotes Colorectal Tumor Metastasis via Dysregulation of MnSOD显示文摘 | Xiangqi Meng Jiangxue Wu Changchuan Pan Hui Wang Xiaofang Ying Yi Zhou Hongyan Yu Yufang Zuo Zhizhong Pan Ran–Yi Liu Wenlin Huang | 2013 | Gastroenterology2013，，2： | 1 |
| 8 | Dry wear behaviors of wear resistant composite coatings produced by laser cladding显示文摘Using different proportional mixtures of Ni-coated MoS2, TiC and pure Ni powders, new typical wear resistant and selflubricant coatings were formed on low carbon steel by laser cladding process. The microstructures and phase composition of the composite coatings were studied by SEM and XRD. The typical microstructure of the composite coating is composed of multisulfide phases including binary element sulfide and ternary element sulfide, γ-Ni, TiC and Mo2C. Wear tests were carried out using an FALEX-6 type pin-on-disc machine. The friction coefficient and mass loss of three kinds of MoS2/TiC/Ni laser clad coatings are lower than those of quenched 45 steel, and the worn surfaces of the laser cladding coatings are very smooth. Because of high hardness combined with low friction, the laser cladding composite coating with a mixture of 70% Ni-coated MoS2, 20%TiC and 10%pure Ni powder presents better wear behaviors than the composite coating with other powder blends. The composition analysis of the worn surface of GCr15 bearing steel shows that the transferred film from the laser cladding coating to the opposite surface of GCr15beating steel contains an amount of sulfide, which can change the micro-friction mechanism and lead to a reduced friction coefficient. | JiangXu WenjinLiu MinlinZhong | 2004 | Journal of University of Science and Technology Beijing2004，11，6： | 1 |
| 9 | The surviving rate of planar graphs 显示文摘 | Kong Jiangxu Wang Weifan Zhu Xuding | 2012 | Theoret Comput Sci2012，416，： | 1 |
| 10 | 显示文摘 | JiangXue(蒋雪) QuWenchao(曲文超) ZhaoJijun(赵纪军)eta1 | 2011 | 中国科学物理学力学天文学2011，41，： | 1 |
| 11 | Structural properties and surviving rate of planar graphs 显示文摘 | Kong Jiangxu Zhang Lianzhu Wang Weifan | 2014 | Discrete Math Algorithms Appl2014，6，14： | 1 |
| 12 | The 2 -surviving rate of planar graphs without 6-cycles 显示文摘 | Waug Weifan Finbow S Kong Jiangxu | 2014 | Theoret Comput Sci2014，518，： | 1 |
| 13 | The surviving rate of digraphs 显示文摘 | Kong Jiangxu Zhang Lianzhu Wang Weifan | 2014 | Discrete Math2014，334，6： | 1 |
| 14 | Acute toxicity and biodistribution of different sized titanium dioxide particles in mice after oral administration显示文摘 | Wang Jiangxue Zhou Guoqiang Chen Chunying | 2007 | Toxicol Lett2007，168，2： | 1 |
| 15 | Expression and purification of human endostatin from Hansenula polymorpha显示文摘 | Wu Jiangxue Fu Wuzhao Luo Jinxian | 2005 | Protein Expression Purification2005，42，： | 1 |
| 16 | Acute toxicity and biodistribution of different sized titanium dioxide particles in mice after oral administration显示文摘 | WANG Jiangxue ZHOU Guoqiang CHEN Chunying | 2007 | Toxicology Letters2007，168，： | 1 |
| 17 | Mobility Support for Content Source in Content-Centric Networking显示文摘Content-centric networking(CCN) is rising to solve the problems suffered by traditional networks,especially in terms of content delivery.One of the critical issues for basic CCN is supporting mobility.Handoff of Mobile Content Source(MCS) will result in a large scale routing update,which generates huge amount of routing overhead and leads to service interruption.Most of the existing CCN mobility mechanisms are transplanted from the IP mobility solutions,which are unnaturally integrated with CCN.Different from previous works,a mobility strategy from the perspective of CCN architecture is proposed to support the handoff of MCS in this paper.Especially,we define the critical network routers that can limit the routing update scale effectively when MCS handoff is conducted.Based on the defined critical network routers,the proposed scheme can provide lower routing update overhead,faster routing convergence and shorter service interruption time.Finally,series of simulations are conducted to validate the effectiveness of our proposed scheme. | HUANG Tao LI Jiangxu LIU Jiang ZHANG Yunyong LIU Yunjie | 2015 | China Communications2015，12，3： | 1 |
| 18 | Potential neurological lesion after nasal instillation of TiO2 nanoparticles in the anatase and rutile crystal phases显示文摘 | Wang Jiangxue Chen Chunying Liu Ying | 2008 | Toxicol Lett2008，183，13： | 1 |
| 19 | Acute toxicity and biodistribution of different sized titanium dioxide particles in mice after oral administration显示文摘 | Jiangxue Wang Guoqiang Zhou Chunying Chen Hongwei Yu Tiancheng Wang Yongmei Ma Guang Jia Yuxi Gao Bai Li Jin Sun Yufeng Li Fang Jiao Yuliang Zhao Zhifang Chai | 2006 | Toxicology Letters2006，，2： | 1 |
| 20 | Surface structure change properties:Auto-soft bionic fibrous membrane in reducing postoperative adhesion显示文摘Peritoneal adhesion is the most common adverse effect following abdominal surgery or inflammation.The occurrence in clinical trials has been successfully reduced using barriers.However,the shortcomings of frequently used adhesion barriers,such as rapid degradation rate of gel barrier and inadequate operation ability of solid barrier,cannot be ignored.In this study,a fibrous membrane with an ECM-like structure was prepared.The adhesion properties were reduced significantly by changing the surface structure.The fibrous membrane caused less inflammatory response and much less peripheral adhesion and intestinal obstruction compared to the casting film and the commercial film with smooth surface,though with the same components.Because of the auto-soft bionic structure and similarity in the mechanical modulus of the tissues,the fibrous membrane was more flexible when it adhered to the tissues,showed excellent effectiveness and biocompatibility.In addition to the rat and miniature pig models,a randomized,placebo-controlled,and multicenter clinical pilot study with 150 patients confirmed that because of its flexibility,biodegradability,and similarity to mechanical modulus and structure with tissues involved,the fibrous membrane served as a favorable implant for preventing post-operation adhesion. | Shanshan Xu Chenhong Wang Ruoqing Mao Xiaoyu Liang Heran Wang Zhenyu Lin Jiangxue Li Shilin Li Jipeng Jiang Tongshuo Zhang Yongfu Ma Yang Liu Charles C.Han Ying Liu | 2022 | Bioactive Materials2022，7，6： | 1 |
您所在的机构暂未开通此部分的功能，请联系管理员