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| 1 | Development of a novel RNAi therapy:Engineered miR-31 exosomes promoted the healing of diabetic wounds显示文摘Rationale:Chronic wounds associated with diabetes exact a heavy burden on individuals and society and do not have a specific treatment.Exosome therapy is an extension of stem cell therapy,and RNA interference(RNAi)-based therapy is a type of advanced precision therapy.Based on the discovery of chronic wound-related genes in diabetes,we combined exosome therapy and RNAi therapy through an engineering approach for the treatment of diabetic chronic wounds.Methods:We combined exosome therapy and RNAi therapy to establish a precision therapy for diabetes-associated wounds via an engineered exosome approach.Results:First,chronic diabetic wounds express low levels of miR-31-5p compared with nondiabetic wounds,and an miR-31-5p mimic was shown to be effective in promoting the proliferation and migration of three wound-related cell types in vitro.Second,bioinformatics analysis,luciferase reporter assays and western blotting suggested that miR-31-5p promoted angiogenesis,fibrogenesis and reepithelization by inhibiting factor-inhibiting HIF-1(HIF1AN,also named FIH)and epithelial membrane protein-1(EMP-1).Third,engineered miR-31 exosomes were generated as a miR-31-5p RNAi therapeutic agent.In vivo,the engineered miR-31 exosomes promoted diabetic wound healing by enhancing angiogenesis,fibrogenesis and reepithelization.Conclusion:Engineered miR-31 exosomes are an ideal disease pathophysiology-initiated RNAi therapeutic agent for diabetic wounds. | Jinghuan Huang Muyu Yu Wenjing Yin Bo Liang Ang Li Jingfeng Li Xiaolin Li Shichang Zhao Fang Liu | 2021 | Bioactive Materials2021，6，9： | 3 |
| 2 | Immunogenicity and protective efficacy of a recombinant protein subunit vaccine and an inactivated vaccine against SARS-CoV-2 variants in non-human primates显示文摘Emerging SARS-CoV-2 variants and the gradually decreasing neutralizing antibodies over time post vaccination have led to an increase in incidents of breakthrough infection across the world.To investigate the potential protective effect of the recombinant protein subunit COVID-19 vaccine targeting receptor-binding domain(RBD)(PS-RBD)and whole inactivated virus particle vaccine(IV)against the variant strains,in this study,rhesus macaques were immunized with PS-RBD or IV vaccine,followed by a Beta variant(B.1.351)challenge.Although neutralizing activity against the Beta variant was reduced compared with that against the prototype,the decreased viral load in both upper and lower respiratory tracts,milder pathological changes,and downregulated inflammatory cytokine levels in lung tissues after challenge demonstrated that PS-RBD and IV still provided effective protection against the Beta variant in the macaque model.Furthermore,PS-RBD-induced macaque sera possessed general binding and neutralizing activity to Alpha,Beta,Delta,and Omicron variants in our study,though the neutralizing antibody(NAb)titers declined by varying degrees,demonstrating potential protection of PS-RBD against current circulating variants of concern(VOCs).Interestingly,although the IV vaccine-induced extremely low neutralizing antibody titers against the Beta variant,it still showed reduction for viral load and significantly alleviated pathological change.Other correlates of vaccine-induced protection(CoP)like antibody-dependent cellular cytotoxicity(ADCC)and immune memory were both confirmed to be existing in IV vaccinated group and possibly be involved in the protective mechanism. | Qian He Qunying Mao Xiaozhong Peng Zhanlong He Shuaiyao Lu Jialu Zhang Fan Gao Lianlian Bian Chaoqiang An Wenhai Yu Fengmei Yang Yanan Zhou Yun Yang Yanyan Li Yadi Yuan Xujia Yan Jinghuan Yang Xing Wu Weijin Huang Changgui Li Junzhi Wang Zhenglun Liang Miao Xu | 2022 | Signal Transduction and Targeted Therapy2022，7，4： | 2 |
| 3 | Preparation of stable micropatterns of gold on cell-adhesion-resistant hydrogels assisted by a hetero-bifunctional macromonomer linker显示文摘Surface patterning is very useful in biomaterial studies, yet it is not easy to prepare a micropattern with cell-adhesion contrast that is stable in a wet environment. Recently, a platform technique of transfer photolithography was invented to fabricate stable metal microarrays on the surface of a cell-adhesion resistant and mechanically biomimetic poly(ethylene glycol) hydrogel; the linker is the key chemical in such a transfer strategy. This article reports the design and synthesis of a hetero-bifunctional macromonomer linker with a thiol group at one end and an acryloyl group at the other end. The bifunctional linker was characterized by GPC and 1H NMR, and the average number of thiol groups in the bifunctional linker was detected by Ellman's reagent. The regent stability under wet conditions was also confirmed by the model reactants. The resultant micropatterned surfaces are meaningful for future studies of cell behaviors on mechanically biomimetic matrixes. | SUN JianGuo GRAETER Stefan V TANG Jian HUANG JingHuan LIU Peng LAI YuXiao YU Lin MAJER Günter SPATZ Joachim P DING JianDong | 2014 | Science China Chemistry2014，57，4： | 2 |
| 4 | 3D visualiz-ation technique of human organs显示文摘 | Song Limei Huang Jinghuan | | 0，，02： | 1 |
| 5 | Effects of polar modifiers on supercritical extraction efficiency for organic template removal from mesoporous MCM -41 materials 显示文摘 | Zhen Huang Li Jinghuan Li Huishan | 2013 | The Journal of Super- critical Fluids2013，，82： | 1 |
| 6 | Organic template removal from hexagonal mesoporous silica by means of methanol - enhanced CO2 extraction : Effect of temperature, pressure and flow rate 显示文摘 | Zhen Huang Li Xu Li Jinghuan | 2011 | Separation and Purification Technology2011，77，1： | 1 |
| 7 | Complete estrogen receptor blocker ICI182,780 promotes the proliferation of vascular smooth muscle cells显示文摘ICI182,780 在乳癌的辅助治疗被使用。作为完全的雌激素受体(嗯) blocker, ICI182,780 可以在心血管的系统上反对雌激素的效果。雌激素禁止脉管的光滑的肌肉房间(VSMC ) 的增长,它是雌激素能施加的机制之一 cardioprotective 效果。在现在的学习,在 VSMC 的增长上估计 ICI182,780 的效果,我们从老鼠主动脉孤立与或没有 ER 对手 ICI182,780 的有教养的 VSMC。3-(4,5-dimethylthiazol-2-yl ) 的结果 -2,5-diphenyltetrazolium 溴化物试金, bromodeoxyuridine 加入试金,可行房间计数,为增殖的房间的免疫化学的染色原子抗原(PCNA ) ,和流动 cytometry 决定的 S 阶段比率在 ICI182,780 治疗以后揭示了 VSMC 的显著增长。ICI182,780 显著地以一种剂量依赖者方式提高了房间生长(10 ? 810 ? 5 M ) 。而且, PCNA 积极的房间的数字和 VSMC 的 S 阶段前进与 ICI182,780 在治疗以后增加了。反向的 transcriptase 聚合酶链反应(RTPCR ) 和西方的污点分析证明在 VSMC 的 cyclin D1 的 mRNA 和蛋白质水平在 ICI182,780 的处理下面增加了。这些数据建议 ICI182,780 能支持 VSMC 的生长,它可能在心血管的系统上生产一些不利效果。 | Hong He Falin Yang Xin Wang Xianwei Zeng Qin Hu Jinghuan Huang Jinbo Feng | 2011 | Acta Biochimica et Biophysica Sinica2011，43，2： | 1 |
| 8 | Engineering of a NIR-activable hydrogel-coated mesoporous bioactive glass scaffold with dual-mode parathyroid hormone derivative release property for angiogenesis and bone regeneration显示文摘Osteogenesis,osteoclastogenesis,and angiogenesis play crucial roles in bone regeneration.Parathyroid hormone(PTH),an FDA-approved drug with pro-osteogenic,pro-osteoclastogenic and proangiogenic capabilities,has been employed for clinical osteoporosis treatment through systemic intermittent administration.However,the successful application of PTH for local bone defect repair generally requires the incorporation and delivery by appropriate carriers.Though several scaffolds have been developed to deliver PTH,they suffer from the weaknesses such as uncontrollable PTH release,insufficient porous structure and low mechanical strength.Herein,a novel kind of NIR-activable scaffold(CBP/MBGS/PTHrP-2)with dual-mode PTHrP-2(a PTH derivative)release capability is developed to synergistically promote osteogenesis and angiogenesis for high-efficacy bone regeneration,which is fabricated by integrating the PTHrP-2-loaded hierarchically mesoporous bioactive glass(MBG)into the N-hydroxymethylacrylamide-modified,photothermal agent-doped,poly(N-isopropylacrylamide)-based thermosensitive hydrogels through assembly process.Upon on/off NIR irradiation,the thermoresponsive hydrogel gating undergoes a reversible phase transition to allow the precise control of on-demand pulsatile and long-term slow release of PTHrP-2 from MBG mesopores.Such NIR-activated dual-mode delivery of PTHrP-2 by this scaffold enables a well-maintained PTHrP-2 concentration at the bone defect sites to continually stimulate vascularization and promote osteoblasts to facilitate and accelerate bone remodeling.In vivo experiments confirm the significant improvement of bone reparative effect on critical-size femoral defects of rats.This work paves an avenue for the development of novel dual-mode delivery systems for effective bone regeneration. | Shi Liu Zhengzhe Han Ji-Na Hao Dapeng Zhang Xianglong Li Yuanyuan Cao Jinghuan Huang Yongsheng Li | 2023 | Bioactive Materials2023，，8： | 1 |
| 9 | Tissue Engineering Materials: Preshaping and Injectable显示文摘 | Dianying Jing Zhen Wang Wen Zhu Junchuan Zhang Shifeng Duan Stefan Graeter Jianguo Sun Jinghuan Huang Huan Zhang Jian Tang Peng Liu Linbo Wu Hong Zhang Jiandong Ding | 2005 | Journal of US-China Medical Science2005，2，4： | 0 |
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