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| 1 | Identification of the candidate genes associated with cellular rejection in pig-to-human xenotransplantation显示文摘To identify the genes associated with cellular rejection in pig-to-human xenotransplantation, the suppression subtractive hybridization (SSH) was used in screening the up-regulated genes from a co-culture of human peripheral blood mononuclear cells (PBMCs) and porcine vascular endothelial cell line PIEC. The up-regulated cDNAs were cloned into pGEM-T Easy vector and then sequenced. Nucleic acid homology searches were performed using the BLAST program. A subtracted cDNA library including about 300 clones with the expected up-regulated genes was obtained. Twenty-four of these clones were analyzed by sequencing and homology comparison was made. These clones represent the genes of human perforin (PRF1), proteasome, lymphocyte specific interferon regulatory factor/interferon regulatory factor 4 (LSIRF/IRF 4), muscleblind-like (MBNL) protein and a porcine expressed sequence tag (EST) which has 81% homology with human oxidative-stress responsive 1 (OSR 1). These genes might be the candidate genes which are associated with cellular rejection in pig-to-human xenotransplantation. | HU Weimin, CHENG Jingqiu, LI Youping , LI Shengfu, LU Xiaofeng and WAN Lin(Laboratory of Transplant Engineering and Immunology, West China Hospital, Sichuan University, Chengdu 610041, China) | 2002 | Progress in Natural Science:Materials International2002，12，9： | 3 |
| 2 | Establishment of a rhesus monkey model of middle cerebral artery ischemia and reperfusion using a microcatheter embolization method显示文摘Nonhuman primates are closest to humans in terms of lineage,and middle cerebral artery ischemia/reperfusion responses of nonhuman primates are most similar to ischemic stroke in humans.Therefore,nonhuman primates could be utilized to simulate the process of ischemic stroke in the human.Few studies,however,have reported the use of endovascular technology to establish a rhesus monkey stroke model.In the present study,seven adult,male,rhesus monkeys were selected and,following anesthesia,a microcatheter was inserted into one side of the middle cerebral artery via the femoral artery to block blood flow,thereby resulting in middle cerebral artery occlusion.After 2 hours,the microcatheter was withdrawn to restore the middle cerebral artery blood flow and to establish ischemia/reperfusion.Results from angiography and magnetic resonance angiography confirmed occlusion and reopening of the middle cerebral artery.Magnetic resonance imaging revealed the existence of ischemic brain lesions,and neurological examination showed sustained functional deficits following surgery.The rhesus monkey middle cerebral artery ischemia/reperfusion models established by microcatheter embolization had the advantage of non-craniotomy invasion and reproducibility.The scope and degree of ischemic damage using this model was controllable.Therefore,this nonhuman primate model is an ideal model for cerebral ischemia and reperfusion. | Jie Yang Jian Guo Hongbo Zheng Muke Zhou Li He Wenying Cao Li Xiao Jiachuan Duan Qiyong Gong Xiaoqi Huang Hongxia Li Li Wang Jingqiu Cheng | 2010 | Neural Regeneration Research2010，5，15： | 2 |
| 3 | Xenozoonoses in xenotransplantation显示文摘Pig to human xenotransplantation is one of the possible ways to solve the problem of organ shortage, but the potential risk of xenozoonoses hinders the progress of xenotransplantation. Among pathogens that might cause xenozoonoses, porcine endogenous retrovirus (PERV) is undoubtedly the most noticeable. Current researches show that PERV exists in pigs' genomes and is able to express in multiple tissues and organs in pigs. Other experiments also show that PERV could successfully infect several human cell lines in vitro and could infect SCID mice through pig to mouse islet transplantation. The discussion and research on the risk of PERV is one of the hottest topics in current xenotransplantation, but the question of whether PERV will transmit to human through xenotransplantation has not been answered due to the lack of suitable animal models. More work should be done to evaluate the risk of xenozoonoses caused by PERV after pig to human xenotransplantation. | ZHANG Li, BU Hong, LI Youping and CHENG Jingqiu(Key Laboratory of Transplant Engineering and Immunology, Ministry of Health, West China Hospital, Sichuan University, Chengdu 610041, China) | 2003 | Progress in Natural Science:Materials International2003，13，1： | 2 |
| 4 | 靶向肾癌中的E3连接酶接头蛋白SPOP的小分子抑制剂显示文摘近年来肾癌发病率上升幅度在恶性肿瘤中排名第一。临床治疗表明,肾癌对放疗和化疗均不敏感,以索拉非尼和舒尼替尼为代表的靶向抗肿瘤药物是晚期肾癌的一线治疗药物,但对转移性肾癌的疗效十分有限,并且容易产生耐药。因此,发现并确证治疗肾癌特异性药物作用新靶标是一项十分紧迫并意义重大的任务。 | Zhong-Qiang Guo Tong Zheng Baoen Chen Cheng Luo Sisheng Ouyang Shouzhe Gong Jiafei Li Liu-Liang Mao Fulin Lian Yong Yang Yue Huang Li Li Jing Lu Bidong Zhang Luming Zhou Hong Ding Zhiwei Gao Liqun Zhou Guoqiang Li Ran Zhou Ke Chen Jingqiu Liu Yi Wen Likun Gong Yuwen Ke Shang-Dong Yang Xiao-Bo Qiu Naixia Zhang Jin Ren Dafang Zhong 杨财广 刘江 蒋华良 | 2017 | 科学新闻2017，19，4： | 1 |
| 5 | The Practical Value of Applying Chemical Biliary Duct Embolization to Chemical Hepatectomy for Treatment of Hepatolithiasis显示文摘 | Fuyu Li Jingqiu Cheng Sheng He Ning Li Mingming Zhang Jiahong Dong Lisheng Jiang Nansheng Cheng Xianzhe Xiong | 2005 | Journal of Surgical Research2005，，2： | 1 |
| 6 | Loss-of-function of sox3 causes follicle development retardation and reduces fecundity in zebrafish显示文摘Folliculogenesis is essential for production of female gametes in vertebrates.However,the molecular mechanisms underlying follicle development,particularly apoptosis regulation in ovary,remain elusive.Here,we gen erated sox3 knockout zebrafish lines using CRISPR/Cas9.sox3 knockout led to follicle development retardation and a reduced fecundity in females.Comparative analysis of transcriptome between sox3^-/-and wild-type ovaries revealed that Sox3 was invoIved in pathways of ovarian steroidogenesis and apoptosis.Knockout of sox3 promoted follicle apoptosis and obvious apoptosis signals were detected in somatic cells of stages III and IV follicles of sox3^-/-ovaries.Moreover,Sox3 can bind to and activate the promoter of cyp19a1a.Up-regulation of Cyp19a1a expression promoted 17β-estradiol synthesis,which inhibited apoptosis in follicle development.Thus,Sox3 functions as a regulator of Cyp19a1a expression,via 17β-E2 linking apoptosis suppression,which is implicated in improving female fecundity. | Qiang Hong Cong Li Ruhong Ying Heming Lin Jingqiu Li Yu Zhao Hanhua Cheng Rongjia Zhou | 2019 | Protein & Cell2019，10，5： | 1 |
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