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| 1 | Mouse miRNA-709 directly regulates miRNA-15a/16-1 biogenesis at the posttranscriptional level in the nucleus: evidence for a microRNA hierarchy system显示文摘MicroRNAs (miRNAs ) 是内长的 noncoding RNA (~ 22 nt ) 在细胞质在 post-transcriptional 水平调整目标基因表示。在房间原子核的要求功能的 argonaute 家庭蛋白质提示了我们假设那 miRNAs 的 miRNAs 和 miRNA 的存在的最近的发现可以也在房间原子核有规章的功能。在这研究,我们证明老鼠 miR-709 主要位于各种各样的房间类型的原子核并且它的原子本地化模式很快在 apoptotic 刺激之上变化。在房间原子核, miR-709 直接在 pri-miR-15a/16-1 上绑在一个 19-nt miR-709 识别元素并且阻止它处理成 pre-miR-15a/16-1,导致 miR-15a/16-1 成熟的抑制。而且,原子 miR-709 通过 miR-15a/16-1 小径参予房间 apoptosis 的规定。在摘要,现在的学习提供一 miRNA 能由直接在原子核指向他们的主要抄本控制另外的 miRNAs 的生物的续生说的第一条证据。 | Rui Tang Limin Li Dihan Zhu Dongxia Hou Ting Cao Hongwei Gu Jing Zhang Junyuan Chen Chen-Yu Zhang Ke Zen | 2012 | Cell Research2012，22，3： | 23 |
| 2 | Structure-activity relationship optimization for lassa virus fusion inhibitors targeting the transmembrane domain of GP2显示文摘Dear Editor,Lassa virus(LASV)belongs to the Mammarenavirus genus,Arenaviridae family.Arenaviruses are classified into two main groups-Old World(OW)and New World(NW)-based on virus genetics,serology,antigenic properties and geographical relationships.The OW LASV and Lujo virus(LUJV),as well as NW Jurn'n virus(JUNV),Machupo virus(MACV),Guanarito virus(GTOV),Sabia virus(SABV)and Chapare virus(CHAPV),are known to cause severe hemorrhagic fever and are listed as biosafety level 4(BSL-4)agents.The arenavirus glycoprotein complex(GPC)contains three subunits-the retained stable-signal peptide(SSP),the receptor-binding subunit GP1,and the membrane fusion subunit GP2(Lenz et al.,2001).Notably,the proximate external membrane region and TM of GP2,together with the ectodomain loop and TMs of SSP,form an SSP-GP2 interface,playing essential roles in regulating membrane fusion,and providing targets for distinct fusion inhibitors(Larson et al.,2008;Lee et al.,2008;York et al.,2008;York and Nunberg,2009;Thomas et al.,2011;Burgeson et al.,2013a;Shankar et al.,2016;Wang et al.,2016;Wang et al.,2018).Among these inhibitors,ST-161 is LASV specific(Burgeson et al.,2013a).In this study,we conducted structure-activity relationship(SAR)optimization of ST-161.As a result,21 derivatives with IC50 values<1 pmol/L are presented in Table S1.Hit compounds 21,29 and 57 exhibiting robust inhibition of the LASV pseudotype virus(LASVpv,VSV backbone enveloped by LASV GPC with single cycle infection)entry with IC50 values lower than 0.2 nmol/L(Figs.1A and S1),as well as hit compound 72 with an ester bond instead of acylhydrazone,were further investigated.To test whether the four hit compounds inhibit LASV entry by blocking the GPC-mediated membrane fusion,the inhibition effects of these compounds against LASV GPC mediated fusion were quantitatively determined by dual-luciferase assay(Thomas et al.,2011;Wang et al.,2018). | Guangshun Zhang Junyuan Cao Yan Cai Yang Liu Yanli Li Peilin Wang Xiaoying Jia Mengmeng Zhang Gengfu Xiao Yu Guo Wei Wang | 2019 | Protein & Cell2019，10，2： | 3 |
| 3 | Regional stretch method to measure the elastic and hyperelastic properties of soft materials显示文摘soft materials;;hyperelasticity;;adhesion;;dimensional analysis;;inverse | JunYuan Sheng HaoYuan Guo YanPing Cao XiQiao Feng | 2018 | Science China(Physics,Mechanics & Astronomy)2018，61，2： | 2 |
| 4 | Nonpharmaceutical interventions contribute to the control of COVID-19 in China based on a pairwise model显示文摘Nonpharmaceutical interventions(NPIs),particularly contact tracing isolation and household quarantine,play a vital role in effectively bringing the Coronavirus Disease 2019(COVID-19)under control in China.The pairwise model,has an inherent advantage in characterizing those two NPIs than the classical well-mixed models.Therefore,in this paper,we devised a pairwise epidemic model with NPIs to analyze COVID-19 outbreak in China by using confirmed cases during February 3rde22nd,2020.By explicitly incorporating contact tracing isolation and family clusters caused by household quarantine,our model provided a good fit to the trajectory of COVID-19 infections.We calculated the reproduction number R=1.345(95%CI:1.230-1.460)for Hubei province and R=1.217(95%CI:1.207-1.227)for China(except Hubei).We also estimated the peak time of infections,the epidemic duration and the final size,which are basically consistent with real observation.We indicated by simulation that the traced high-risk contacts from incubated to susceptible decrease under NPIs,regardless of infected cases.The sensitivity analysis showed that reducing the exposure of the susceptible and increasing the clustering coefficient bolster COVID-19 control.With the enforcement of household quarantine,the reproduction number R and the epidemic prevalence declined effectively.Furthermore,we obtained the resumption time of work and production in China(except Hubei)on 10th March and in Hubei at the end of April 2020,respectively,which is broadly in line with the actual time.Our results may provide some potential lessons from China on the control of COVID-19 for other parts of the world. | Xiao-Feng Luo Shanshan Feng Junyuan Yang Xiao-Long Peng Xiaochun Cao Juping Zhang Meiping Yao Huaiping Zhu Michael Y.Li Hao Wang Zhen Jin | 2021 | Infectious Disease Modelling2021，6，1： | 2 |
| 5 | Characterizing the Lassa Virus Envelope Glycoprotein Membrane Proximal External Region for Its Role in Fusogenicity显示文摘The membrane-proximal external region(MPER)of Lassa virus(LASV)glycoprotein complex(GPC)is critical in modulating its functionality.Till now,the high-resolution structure of the intact GPC,including MPER is not available.In this study,we used alanine substitution to scan all 16 residues located in LASV MPER.Western blotting and quantification fusion assay showed that the residues located at the C terminus of the HR2(M414 and L415)and N terminus of the MPER(K417 and Y419)are critical for GPC-mediated membrane fusion function.Furthermore,cell surface biotinylation experiments revealed that M414 A,K417 A and Y419 A expressed similar levels as WT,whereas L415 A mutant led to a reduction of mature GPC on the cell surface.Moreover,substitution of these residues with the similar residue such as M414 L,L415 I,K417 R and Y419 F would partly compensate the loss of the fusion activity caused by the alanine mutant in these sites.Results from this study showed that several key residues in the MPER region are indispensable to promote the conformational changes that drive fusion events and shed light on the structure analysis of LASV GPC and anti-LASV therapeutics. | Junyuan Cao Guangshun Zhang Minmin Zhou Yang Liu Gengfu Xiao Wei Wang | 2021 | Virologica Sinica2021，36，2： | 1 |
| 6 | FPSO Global Strength and Hull Optimization显示文摘Global strength is a significant item for floating production storage and offloading(FPSO) design, and steel weight plays an important role in the building costs of FPSO. It is the main task to consider and combine these two aspects by optimizing hull dimensions. There are many optional methods for the global strength analysis. A common method is to use the ABS FPSO Eagle software to analyze the global strength including the rule check and direct strength analysis. And the same method can be adopted for the FPSO hull optimization by changing the depth. After calculation and optimization, the results are compared and analyzed. The results can be used as a reference for the future design or quotation purpose. | Junyuan Ma Jianhua Xiao Rui Ma Kai Cao | 2014 | Journal of Marine Science and Application2014，13，1： | 1 |
| 7 | Mitochondrial uncoupling protein 2 protects splenocytes from oxidative stress-induced apoptosis during pathogen activation显示文摘 | Ting Cao Yeyan Dong Rui Tang Junyuan Chen Chen-Yu Zhang Ke Zen | 2013 | 2013 (1-2)2013，，1： | 1 |
| 8 | Effects of N-Linked Glycan on Lassa Virus Envelope Glycoprotein Cleavage,Infectivity,and Immune Response显示文摘Lassa virus(LASV)belongs to the Mammarenavirus genus(family Arenaviridae)and causes severe hemorrhagic fever in humans.The glycoprotein complex(GPC)contains eleven N-linked glycans that play essential roles in GPC functionalities such as cleavage,transport,receptor recognition,epitope shielding,and immune response.We used three mutagenesis strategies(asparagine to glutamine,asparagine to alanine,and serine/tyrosine to alanine mutants)to abolish individual glycan chain on GPC and found that all the three strategies led to cleavage inefficiency on the 2nd(N89),5th(N119),or 8th(N365)glycosylation motif.To evaluate N to Q mutagenesis for further research,it was found that deletion of the 2nd(N89Q)or 8th(N365Q)glycan completely inhibited the transduction efficiency of pseudotyped particles.We further investigated the role of individual glycan on GPC-mediated immune response by DNA immunization of mice.Deletion of the individual 1st(N79Q),3rd(N99Q),5th(N119Q),or 6th(N167Q)glycan significantly enhanced the proportion of effector CD4+cells,whereas deletion of the 1st(N79Q),2nd(N89Q),3rd(N99Q),4th(N109Q),5th(N119Q),6th(N167Q),or 9th(N373Q)glycan enhanced the proportion of CD8+effector T cells.Deletion of specific glycan improves the Th1-type immune response,and abolishment of glycan on GPC generally increases the antibody titer to the glycan-deficient GPC.However,the antibodies from either the mutant or WT GPC-immunized mice show little neutralization effect on wild-type LASV.The glycan residues on GPC provide an immune shield for the virus,and thus represent a target for the design and development of a vaccine. | Xueqin Zhu Yang Liu Jiao Guo Junyuan Cao Zonglin Wang Gengfu Xiao Wei Wang | 2021 | Virologica Sinica2021，36，4： | 0 |
| 9 | Formation of sulfur oxide groups by SO_(2) and their roles in mercury adsorption on carbon-based materials显示文摘The presence of SO_(2) display significant effect on the mercury(Hg) adsorption ability of carbon-based sorbent. Yet the adsorption and oxidation of SO_(2) on carbon with oxygen group, as well as the roles of different sulfur oxide groups in Hg adsorption have heretofore been unclear. The formation of sulfur oxide groups by SO_(2) and their effects on Hg adsorption on carbon was detailed examined by the density functional theory. The results show that SO_(2) can be oxidized into SO_(2) by oxygen group on carbon surface. Both C-SO_(2) and C-SO_(2)can improve Hg adsorption on carbon site, while the promotive effect of C-SO_(2) is stronger than C-SO_(2). Electron density difference analyses reveal that sulfur oxide groups enhance the charge transfer ability of surface unsaturated carbon atom, thereby improving Hg adsorption. The experimental results confirm that surface active groups formed by SO_(2) adsorption is more active for Hg adsorption than the groups generated by SO_(2). | Fenghua Shen Shudan He Junyuan Li Cao Liu Kaisong Xiang Hui Liu | 2022 | Journal of Environmental Sciences2022，34，9： | 0 |
| 10 | Significantly enhanced energy density of nanodiamond/polyimide composites at high temperatures with ultralow nanodiamond contents显示文摘The development of miniature devices has generated a large demand for dielectric polymers with high energy storage density,especially at high temperatures.First,poly(methyl methacrylate)(PMMA)-coated nanodiamond(ND)particles were incorporated into a polyimide(PI)substrate to fabricate ND-g-PMMA/PI(NPP)composites.We found for the first time that with a very low content(0.4 vol.%)of ND particles,NPP-0.4 composite material gave the dielectric constant of 4.4,which is 37.5%higher than that of the PI film.The maximum energy density of the NPP-0.4 composite film achieves 9.4 J cm^(-3)at room temperature,which is 54%higher than that of the PI film.Furthermore,we found that the NPP-0.2 composite reaches the highest energy density and discharge efficiency of 5.77 J cm^(-3)and 72.3%at 150℃,which are 104%and 51.3%higher than those of the PI film at 150℃.This result shows a much higher energy density than most previously published results for high-temperature polymer capacitors.This phenomenon can be explained by the simulation results using COMSOL in which the interface polarization of ND/PI composites reached their maximum value with ultralow ND contents(0.4 vol.%).This research shows great promise for polymer capacitors with relatively high energy density at high temperatures. | WANG ChengWei CAO XiaoDan TIAN JunYuan YU Fang JIANG FengYing REN KaiLiang | 2023 | Science China(Technological Sciences)2023，66，4： | 0 |
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