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| 1 | Chinese Multidisciplinary Expert Consensus on the Diagnosis and Treatment of Hyperuricemia and Related Diseases显示文摘 | Zou, He-Jian Wu, Hu-Sheng Zhou, Jing-Guo Zeng, Xue-Jun Dai, Lie Wu, Hua-Xiang Zhu, Xiao-Xia Mei, Chang-Lin Hao, Chuan-Ming Chen, Nan Liu, Bi-Cheng Chen, Jiang-Hua Yang, Li Nie, Jing Yu, Chen Peng, Ai Yu, Sheng-Qiang Li, Lin Ge, Jun-Bo Huo, Yong Zhang, Shu-Yang Chen, Yun-Dai Dong, Yu-Gang Liang, Chun Dai, Yu-Xiang Gao, Xin Li, Chang-Gui Zhao, Jia-Jun Chen, Hai-Bing Cheng, Zhi-Feng Lin, Huan-Dong Guan, Yang-Tai Wang, Kai Luo, Ben-Yan Dai, Ruo-Lian Jiang, Quan Xue, Luan Liang, Chao-Chao Chen, Ming Fan, Song | 2017 | Chinese Medical Journal2017，，20： | 30 |
| 2 | Eleven COVID-19 Outbreaks with Local Transmissions Caused by the Imported SARS-CoV-2 Delta VOC-China,July-August,2021显示文摘Starting from December 2019,Wuhan,China,encountered the first outbreak of coronavirus disease 2019(COVID-19)(1-2).The epidemic was successfully suppressed by strict containment so that the number of infected people was reduced to 0 on April 8,2020(3–4).After that,China experienced roughly 3 dozen outbreaks with local transmission caused by imported severe acute respiratory syndrome coronavirus 2(SARS-CoV-2). | Lei Zhou Kai Nie Hongting Zhao Xiang Zhao Bixiong Ye Ji Wang Cao Chen Hong Wang Jiangli Di Jinsong Li Chao Li Zhixiao Chen Ruiqi Ren Peihua Niu Hui Chen Tao Chen Yali Wang Lili Li Bingxue Han Ruhan A Si Chen Dan Li Dan Li Chunxia Jin Xin Zhang Jiangmei Liu Chunyu Xu Yecheng Yao Yenan Feng Zhili Li Bike Zhang Yang Song Peter Hao Yanping Zhang Hao Li Qun Li George F.Gao Guoqing Shi Wenbo Xu | 2021 | China CDC weekly2021，3，41： | 13 |
| 3 | Methylprednisolone promotes recovery of neurological function after spinal cord injury: association with Wnt/β-catenin signaling pathway activation显示文摘Some studies have indicated that the Wnt/β-catenin signaling pathway is activated following spinal cord injury,and expression levels of specific proteins,including low-density lipoprotein receptor related protein-6 phosphorylation,β-catenin,and glycogen synthase kinase-3β,are significantly altered.We hypothesized that methylprednisolone treatment contributes to functional recovery after spinal cord injury by inhibiting apoptosis and activating the Wnt/β-catenin signaling pathway.In the current study,30 mg/kg methylprednisolone was injected into rats with spinal cord injury immediately post-injury and at 1 and 2 days post-injury.Basso,Beattie,and Bresnahan scores showed that methylprednisolone treatment significantly promoted locomotor functional recovery between 2 and 6 weeks post-injury.The number of surviving motor neurons increased,whereas the lesion size significantly decreased following methylprednisolone treatment at 7 days post-injury.Additionally,caspase-3,caspase-9,and Bax protein expression levels and the number of apoptotic cells were reduced at 3 and 7days post-injury,while Bcl-2 levels at 7 days post-injury were higher in methylprednisolone-treated rats compared with saline-treated rats.At 3 and 7 days post-injury,methylprednisolone up-regulated expression and activation of the Wnt/β-catenin signaling pathway,including low-density lipoprotein receptor related protein-6 phosphorylation,β-catenin,and glycogen synthase kinase-3β phosphorylation.These results indicate that methylprednisolone-induced neuroprotection may correlate with activation of the Wnt/β-catenin signaling pathway. | Gong-biao Lu Fu-wen Niu Ying-chun Zhang Lin DU Zhi-yuan Liang Yuan Gao Ting-zhen Yan Zhi-kui Nie Kai Gao | 2016 | Neural Regeneration Research2016，11，11： | 11 |
| 4 | TaqMan Real-time RT-PCR Assay for Detecting and Differentiating Japanese Encephalitis Virus显示文摘Objective To detect Japanese encephalitis virus(JEV) rapidly and distinguish its genotypes, a TaqMan-based reverse transcriptase quantitative polymerase chain reaction(RT-PCR) detection system was developed.Methods By aligning the full-length sequences of JEV(G1-G5), six sets of highly specific TaqMan real-time RT-PCR primers and probes were designed based on the highly conserved NS1, NS2, and M genes of JEV, which included one set for non-specific JEV detection and five sets for the detection of specific JEV genotypes. Twenty batches of mosquito samples were used to evaluate our quantitative PCR assay.Results With the specific assay, no other flavivirus were detected. The lower limits of detection of the system were 1 pfu/mL for JEV titers and 100 RNA copies/μL. The coefficients of variation of this real-time RT-PCR were all < 2.8%. The amplification efficiency of this method was between 90% and 103%.Conclusion A TaqMan real-time RT-PCR detection system was successfully established to detect and differentiate all five JEV genotypes. | SHAO Nan LI Fan NIE Kai FU Shi Hong ZHANG Wei Jia HE Ying LEI Wen Wen WANG Qian Ying LIANG Guo Dong CAO Yu Xi WANG Huan Yu | 2018 | Biomedical and Environmental Sciences2018，31，3： | 10 |
| 5 | Risk factors of intra-abdominal bacterial infection after liver transplantation in patients with hepatocellular carcinoma显示文摘Objective: To explore the risk factors of intra-abdominal bacterial infection(IAI) after liver transplantation(LT) in patients with hepatocellular carcinoma(HCC). Methods: A series of 82 HCC patients who received LT surgeries in our department between March 2004 and April 2010 was recruited in this study. Then we collected and analyzed the clinical data retrospectively. Statistical analysis system(SPSS) software was adopted to perform statistical analysis. Chi-square test, t-test and Wilcoxon rank sum test were used to analyze the clinical data and compute the significance of the incidences of early-stage IAI after LT for HCC patients. Binary logistic regression was performed to screen out the risk factors, and multiple logistic regression analyses were performed to compute the independent risk factors. Results: A series of 13 patients(13/82, 15.9%) had postoperative IAI. The independent risk factors of postoperative intra-abdominal bacterial infections after LT for HCC patients were preoperative anemia [Hemoglobin(HGB) <90 g/L] and postoperative abdominal hemorrhage(72 hours >400 mL), with the odds ratios at 8.121(95% CI, 1.417 to 46.550, P=0.019) and 5.911(95% CI, 1.112 to 31.432, P=0.037). Conclusions: Postoperative IAI after LT in patients with HCC was a common complication. Preoperative moderate to severe anemia, as well as postoperative intra-abdominal hemorrhage more than 400 mL within the first 72 hours might independently indicate high risk of IAI for these patients. | Kai Nie Rongzheng Ran Weifeng Tan Bin Yi Xiangji Luo Yong Yu Xiaoqing Jiang | 2014 | Chinese Journal of Cancer Research2014，26，3： | 10 |
| 6 | The Two Reemergent Confirmed COVID-19 Cases--Manzhouli City,Inner Mongolia Autonomous Region,China,November 20,2020显示文摘On November 20,2020,a 55-year-old male went to the Zhongmeng Hospital of Manzhouli City,Inner Mongolia Autonomous Region for diagnosis and treatment of pain in the waist and lower extremities.His wife,a 55-year-old female,accompanied him to the hospital also feeling unwell.They were first tested for coronavirus disease 2019(COVID-19)in Manzhouli People’s Hospital based on China’s policy of ensuring that“all those in need are tested,isolated,hospitalized,and treated.” | Xiaoling Tian Yang Song Kai Nie Guodong Wang Hongquan Wang Wenbo Xu Wenrui Wang | 2020 | China CDC weekly2020，2，51： | 8 |
| 7 | A Case of COVID-19 Detected in a Cargo Worker at Pudong Airport-Shanghai Municipality, China, November 8, 2020显示文摘On November 8,2020,a 51-year-old male went to the fever clinic of Shanghai Pudong New Area People’s Hospital for diagnosis and treatment of symptoms and was tested for coronavirus disease 2019(COVID-19).In the early hours of November 9,the municipal and district-level CDCs determined his nucleic acid test results were positive,and after consultation with experts,comprehensive clinical examination,and inspection of imaging results,the COVID-19 diagnosis was confirmed. | Fanghao Fang Yang Song Lipeng Hao Kai Nie Xiaodong Sun | 2020 | China CDC weekly2020，2，47： | 8 |
| 8 | An Improved Barcoded Oligonucleotide Primers-based Next-generation Sequencing Approach for Direct Identification of Viral Pathogens in Clinical Specimens显示文摘Objective To provide a feasible and cost-effective next-generation sequencing(NGS) method for accurate identification of viral pathogens in clinical specimens, because enormous limitations impede the clinical use of common NGS, such as high cost, complicated procedures, tremendous data analysis, and high background noise in clinical samples. Methods Viruses from cell culture materials or clinical specimens were identified following an improved NGS procedure: reduction of background noise by sample preprocessing, viral enrichment by barcoded oligonucleotide(random hexamer or non-ribosomal hexanucleotide) primer-based amplification, fragmentation-free library construction and sequencing of one-tube mixtures, as well as rapid data analysis using an in-house pipeline. Results NGS data demonstrated that both barcoded primer sets were useful to simultaneously capture multiple viral pathogens in cell culture materials or clinical specimens and verified that hexanucleotide primers captured as many viral sequences as hexamers did. Moreover, direct testing of clinical specimens using this improved hexanucleotide primer-based NGS approach provided further detailed genotypes of enteroviruses causing hand, foot, and mouth disease(HFMD) and identified other potential viruses or differentiated misdiagnosis events. Conclusion The improved barcoded oligonucleotide primer-based NGS approach is simplified, time saving, cost effective, and appropriate for direct identification of viral pathogens in clinical practice. | WANG Churl Hua NIE Kai ZHANG Yi WANG Ji ZHOU Shuai Feng LI Xin Na ZHOU Hang Yu QI Shun Xiang MA Xue Jun | 2017 | Biomedical and Environmental Sciences2017，30，1： | 7 |
| 9 | Genome Characterization of the First Outbreak of COVID-19 Delta Variant B.1.617.2——Guangzhou City,Guangdong Province,China,May 2021显示文摘On May 20,2021,75-year-old female(Case A)went to a local hospital due to pharyngeal pain and low-grade fever in Liwan District,Guangzhou City,Guangdong Province.The oropharyngeal swab from Case A tested preliminarily positive for severe acute respiratory syndrome coronavirus 2(SARS-CoV-2)/coronavirus disease 2019(COVID-19)virus using a quantitative real-time reverse transcription polymerase chain reaction(RT-qPCR)method after a hospital visit. | Zhencui Li Kai Nie Kuibiao Li Yao Hu Yang Song Min Kang Meng Zhang Xiaoling Deng Jun Yuan Wenbo Xu Baisheng Li | 2021 | China CDC weekly2021，3，27： | 7 |
| 10 | A highly porous animal bone-derived char with a superiority of promoting nZVI for Cr(Ⅵ)sequestration in agricultural soils显示文摘Paddy soil and irrigation water are commonly contaminated with hexavalent chromium[Cr(Ⅵ)]near urban industrial areas,thereby threatening the safety of agricultural products and human health.In this study,we develop a porous and high specific area bone char(BC)to support nanoscale zero-valent iron(n ZVI)and apply it to remediate Cr(Ⅵ)pollution in water and paddy soil under anaerobic conditions.The batch experiments reveal that BC/n ZVI exhibits a higher removal capacity of 516.7 mg/(g·n ZVI)for Cr(Ⅵ)than n ZVI when normalized to the actual n ZVI content,which is 2.8 times that of n ZVI;moreover,the highest n ZVI utilization is the n ZVI loading of 15%(BC/n ZVI15).The Cr(Ⅵ)removal efficiency of BC/n ZVI15 decreases with increasing p H(4–10).Coexisting ions(phosphate and carbonate)and humic acid can inhibit the removal of Cr(Ⅵ)with BC/n ZVI15.Additionally,BC exhibits a strong advantage in promoting Cr(Ⅵ)removal by n ZVI compared to the widely used biochar and activated carbon.Our results demonstrate that reduction and coprecipitation are the dominant Cr(Ⅵ)removal mechanisms.Furthermore,BC/n ZVI15 shows a significantly higher reduction and removal efficiency as well as a strong anti-interference ability for Cr(Ⅵ)in paddy soil,as compared to n ZVI.These findings provide a new effective material for remediating Cr(Ⅵ)pollution from water and soil. | Kai Liu Fangbai Li Qingwen Tian Chengrong Nie Yibing Ma Zhenlong Zhu Liping Fang Yuanying Huang Siwen Liu | 2021 | Journal of Environmental Sciences2021，33，6： | 7 |
| 11 | Risk Factors for Strangulated Ovarian Hernia in Female Infants:the Role of Ovarian Volume显示文摘The risk factors associated with strangulated ovarian hernia (SOH)in female patients (<1 year old)were identified.A retrospective analysis was conducted regarding the data from 2006 to 2017.The patients were divided into 2groups:SOH group (n=9)and non-SOH group (n=23). Patient demographics,clinical signs,preoperative examinations and intraoperative findings were compared between the two groups,and risk factors for SOH were tested using a binary logistic regression model.To explore whether greater ovary was more likely to be twisted,leading to SOH, all the patients were divided into ovary volume <5cm^3 and ≥5cm^3 groups and the association between ovarian volume and ovary torsion was assessed.Among a total of 32 female patients (<1year old)with incarcerated ovarian herniation,9 patients developed SOH.The single variate analysis revealed that times of manual reduction,ovarian volume,ovary with or without multiple cysts,ovary torsion or not and angle of ovary torsion were found to be significant factors associated with SOH.The multivariate analysis showed ovarian volume was evidenced as an independent risk factor for SOH.Furthermore,the incidence of ovary torsion was significantly higher in ovarian volume ≥5cm^3 group than in ovarian volume <5cm^3 group,indicating that larger ovary was more likely to result in ovary torsion,leading to SOH.Our study demonstrated that the odds of SOH increased with increasing ovarian volume in female patients (<1 year old)because the relatively greater ovary at this age was more likely to be incarcerated and twisted,leading to SOH. | Yu CHEN Xiang-zhi PENG Wei LU Kai ZHENG Jian GUO Hua NIE Xiao-jie SONG Yan ZHANG Jing YANG | 2018 | Current Medical Science2018，38，6： | 6 |
| 12 | The First Case of Asymptomatic Infection of COVID-19-Kashgar Prefecture, Xinjiang Uygur Autonomous Region, China, October, 2020显示文摘On October 24,2020,a 17-year-old female was detected as having an asymptomatic infection of coronavirus disease 2019(COVID-19)during a regular nucleic acid test in Kashgar Prefecture,Xinjiang Uygur Autonomous Region.She was then transferred to a local designated hospital for medical observation.The patient lived in Shufu County of Kashgar Prefecture and worked in a local garment factory.On the afternoon of October 24 after she was diagnosed as positive,a total of 831 persons in the garment factory were tested and the results were all negative.Afterwards,16 close contacts and 406 of close contacts of close contacts were also tested,and 137 were positive and had asymptomatic infections. | Ji Wang Jun Zhao Xiang Zhao Zhenguo Gao Yang Song Xin Ma Kai Nie Aimin Xu Mahemuti Ling Zhang Wenbo Xu Yan Cui | 2020 | China CDC weekly2020，2，45： | 6 |
| 13 | Hedgehog signaling in gastrointestinal carcinogenesis and the gastrointestinal tumor microenvironment显示文摘The Hedgehog(HH) signaling pathway plays important roles in gastrointestinal carcinogenesis and the gastrointestinal tumor microenvironment(TME). Aberrant HH signaling activation may accelerate the growth of gastrointestinal tumors and lead to tumor immune tolerance and drug resistance.The interaction between HH signaling and the TME is intimately involved in these processes, for example, tumor growth, tumor immune tolerance, inflammation, and drug resistance. Evidence indicates that inflammatory factors in the TME, such as interleukin 6(IL-6) and interferon-g(IFN-g), macrophages, and T cell-dependent immune responses, play a vital role in tumor growth by affecting the HH signaling pathway. Moreover, inhibition of proliferating cancer-associated fibroblasts(CAFs) and inflammatory factors can normalize the TME by suppressing HH signaling. Furthermore, aberrant HH signaling activation is favorable to both the proliferation of cancer stem cells(CSCs) and the drug resistance of gastrointestinal tumors. This review discusses the current understanding of the role and mechanism of aberrant HH signaling activation in gastrointestinal carcinogenesis, the gastrointestinal TME,tumor immune tolerance and drug resistance and highlights the underlying therapeutic opportunities. | Jinghui Zhang Jiajun Fan Xian Zeng Mingming Nie Jingyun Luan Yichen Wang Dianwen Ju Kai Yin | 2021 | Acta Pharmaceutica Sinica B2021，11，3： | 5 |
| 14 | Salidroside Protects Against 6-Hydroxydopamine-Induced Cytotoxicity by Attenuating ER Stress显示文摘Parkinson's disease(PD) is a neurodegenerative disease characterized by a persistent decline of dopaminergic(DA) neurons in the substantia nigra pars compacta. Despite its frequency, effective therapeutic strategies that halt the neurodegenerative processes are lacking, reinforcing the need to better understand the molecular drivers of this disease. Importantly, increasing evidence suggests that the endoplasmic reticulum(ER)stress-induced unfolded protein response is likely involved in DA neuronal death. Salidroside, a major compound isolated from Rhodiola rosea L., possesses potent antioxidative stress properties and protects against DA neuronal death. However, the underlying mechanisms are not well understood. In the present study, we demonstrate that salidroside prevents 6-hydroxydopamine(6-OHDA)-induced cytotoxicity by attenuating ER stress. Furthermore, treatment of a DA neuronal cell line(SN4741) and primary cortical neurons with salidroside significantly reduced neurotoxin-induced increases in cytoplasmic reactive oxygen species and calcium, both of which cause ER stress, and cleaved caspase-12, which is responsible for ER stress-induced cell death. Together, these results suggest that salidroside protects SN4741 cells and primary cortical neurons from 6-OHDA-induced neurotoxicity by attenuating ER stress. This provides a rationale for the investigation of salidroside as a potential therapeutic agent in animal models of PD. | Kai Tao Bao Wang Dayun Feng Wei Zhang Fangfang Lu Juan Lai Lu Huang Tiejian Nie Qian Yang | 2016 | Neuroscience Bulletin2016，32，1： | 5 |
| 15 | Good laboratory practices guarantee biosafety in the Sierra Leone-China friendship biosafety laboratory显示文摘Background:The outbreak of Ebola virus disease(EVD)in West Africa between 2014 and 2015 was the largest EDV epidemic since the identification of Ebola virus(EBOV)in 1976,and the countries most strongly affected were Sierra Leone,Guinea,and Liberia.Findings:The Sierra Leone-China Friendship Biological Safety Laboratory(SLE-CHN Biosafety Lab),a fixed Biosafety Level 3 laboratory in the capital city of Sierra Leone,was established by the Chinese government and has been active in EBOV detection since 11 March 2015.Complete management and program documents were created for the SLE-CHN Biosafety Lab,and it was divided into four zones(the green,yellow,brown,and red zones)based on the risk assessment.Different types of safe and appropriate personnel protection equipment(PPE)are used in different zones of the laboratory,and it fully meets the Biosafety Level 3 laboratory standards of the World Health Organization.Conclusion:Good preparedness,comprehensive risk assessment and operation documents,appropriate PPE,effective monitoring and intensive training,together with well-designed and reasonable laboratory sectioning are essential for guaranteeing biosafety. | Qin Wang Wei-Min Zhou Yong Zhang Huan-Yu Wang Hai-Jun Du Kai Nie Jing-Dong Song Kang Xiao Wen-Wen Lei Jian-Qiang Guo He-Jiang Wei Kun Cai Yan-Hai Wang Jiang Wu Gerard Kamara Idrissa Kamara Qiang Wei Mi-Fang Liang Gui-Zhen Wu Xiao-Ping Dong | 2016 | Infectious Diseases of Poverty2016，5，1： | 5 |
| 16 | Engineered Functional Surfaces by Laser Microprocessing for Biomedical Applications显示文摘Metallic biomaterials are increasingly being used in various medical applications due to their high strength,fracture resistance,good electrical conductivity,and biocompatibility.However,their practical applications have been largely limited due to poor surface performance.Laser microprocessing is an advanced method of enhancing the surface-related properties of biomaterials.This work demonstrates the capability of laser microprocessing for biomedical metallic materials including magnesium and titanium alloys,with potential applications in cell adhesion and liquid biopsy.We investigate laser-material interaction,microstructural evolution,and surface performance,and analyze cell behavior and the surface-enhanced Raman scattering(SERS)effect.Furthermore,we explore a theoretical study on the laser microprocessing of metallic alloys that shows interesting results with potential applications.The results show that cells exhibit good adhesion behavior at the surface of the laser-treated surface,with a preferential direction based on the textured structure.A significant SERS enhancement of 6×10^3 can be obtained at the laser-textured surface during Raman measurement. | Guoqing Hu Kai Guan Libin Lu Jiaru Zhang Nie Lu Yingchun Guan | 2018 | Engineering2018，4，6： | 5 |
| 17 | Design and Optimization of a Single Stage Centrifugal Compressor for a Solar Dish-Brayton System显示文摘According to the requirements of a solar dish-Brayton system,a centrifugal compressor stage with a minimum total pressure ratio of 5,an adiabatic efficiency above 75% and a surge margin more than 12% needs to be designed.A single stage,which consists of impeller,radial vaned diffuser,90° crossover and two rows of axial stators,was chosen to satisfy this system.To achieve the stage performance,an impeller with a 6:1 total pressure ratio and an adiabatic efficiency of 90% was designed and its preliminary geometry came from an in-house one-dimensional program.Radial vaned diffuser was applied downstream of the impeller.Two rows of axial stators after 90° crossover were added to guide the flow into axial direction.Since jet-wake flow,shockwave and boundary layer separation coexisted in the impeller-diffuser region,optimization on the radius ratio of radial diffuser vane inlet to impeller exit,diffuser vane inlet blade angle and number of diffuser vanes was carried out at design point.Finally,an optimized centrifugal compressor stage fulfilled the high expectations and presented proper performance.Numerical simulation showed that at design point the stage adiabatic efficiency was 79.93% and the total pressure ratio was 5.6.The surge margin was 15%.The performance map including 80%,90% and 100% design speed was also presented. | Yongsheng Wang Kai Wang Zhiting Tong Feng Lin Chaoqun Nie Abraham Engeda | 2013 | Journal of Thermal Science2013，22，5： | 5 |
| 18 | Re-isolation of Wuxiang Virus from Wild Sandflies Collected from Yangquan County, China显示文摘We previously isolated a new species of the genus Phlebovirus from wild sandflies collected from Wuxiang County in central China,which named the Wuxiang virus(WUXV).In this study,we re-isolated the WUXV from wild sandflies collected from two villages in Yangquan County,China in 2019.Four virus isolates that caused cytopathic effects in BHK-21 cells were successfully isolated from sandfly specimens collected from chicken pens and sheep pens.Phylogenetic analyses of the L,M and S gene segments of the viruses revealed that the four virus strains represented the previously isolated WUXV.The minimum infection rate(MIR)of the virus isolated from the sheep pen was 3.21,and the MIR of the virus isolated from the chicken pen was 3.45.The positive rates of Wuxiang virus neutralizing antibodies in serum samples of local healthy people and domestic chickens were 8.7%(4/46)and 100%(4/4),respectively,suggesting that Wuxiang virus can infect human and animal.In view of the fact that Wuxiang virus is infectious to humans and animals and has a relatively wide geographical distribution in China,it is of great public health significance to strengthen the investigation and study on the infection status of Wuxiang virus in humans and animals. | Qinyan Wang Shihong Fu Jingxia Cheng Xiuyan Xu Jing Wang Bin Wu Xiaodong Tian Yan Li Ying He Fan Li Kai Nie Songtao Xu Bin Wang Huanyu Wang Xiaoqing Lu Guodong Liang | 2021 | Virologica Sinica2021，36，5： | 4 |
| 19 | Critical function of DNA methyltransferase 1 in tomato development and regulation of the DNA methylome and transcriptome显示文摘DNA methylation confers epigenetic regulation on gene expression and thereby on various biological processes.Tomato has emerged as an excellent system to study the function of DNA methylation in plant development.To date,regulation and function of DNA methylation maintenance remains unclear in tomato plants.Here,we report the critical function of tomato(Solanum lycopersicum)Methyltransferase 1(Sl MET1)in plant development and DNA methylome and transcriptome regulation.Using CRISPR-Cas9 gene editing,we generated slmet1 mutants and observed severe developmental defects with a frame-shift mutation,including small and curly leaves,defective inflorescence,and parthenocarpy.In leaf tissues,mutations in Sl MET1 caused CG hypomethylation and CHH hypermethylationon a whole-genome scale,leading to a disturbed transcriptome including ectopic expression of many RIN target genes such as ACC2 in leaf tissues,which are normally expressed in fruits.Neither the CG hypomethylation nor CHH hypermethylation in the slmet1 mutants is related to tissue culture.Meanwhile,tissue culture induces non-CG hypomethylation,which occurs more frequently at gene regions than at TE regions.Our results depict Sl MET1-and tissue culture-dependent tomato DNA methylomes,and that Sl MET1 is required for maintaining a normal transcriptome and normal development of tomato. | Yu Yang Kai Tang Tatsiana U Datsenka Wenshan Liu Suhui Lv Zhaobo Lang Xingang Wang Jinghui Gao Wei Wang Wenfeng Nie Zhaoqing Chu Heng Zhang Avtar K HANDa Jian-Kang Zhu Huiming Zhang | 2019 | Journal of Integrative Plant Biology2019，61，12： | 3 |
| 20 | The LRXs-RALFs-FER module controls plant growth and salt stress responses by modulating multiple plant hormones显示文摘Salt stress is a major environmental factor limiting plant growth and productivity.We recently discovered an important new salt tolerance pathway,where the cell wall leucine-rich repeat extensins LRX3/4/5,the RAPID ALKALINIZATION FACTOR(RALF)peptides RALF22/23 and receptor-like kinase FERONIA(FER)function as a module to simultaneously regulate plant growth and salt stress tolerance.However,the intracellular signaling pathways that are regulated by the extracellular LRX3/4/5-RALF22/23-FER module to coordinate growth,cell wall integrity and salt stress responses are still unknown.Here,we report that the LRX3/4/5-RALF22/23-FER module negatively regulates the levels of jasmonic acid(JA),salicylic acid(SA)and abscisic acid(ABA).Blocking JA pathway rescues the dwarf phenotype of the lrx345 and fer-4mutants,while disruption of ABA biosynthesis suppresses the salt-hypersensitivity of these mutants.Many salt stress-responsive genes display abnormal expression patterns in the lrx345 and fer-4 mutants,as well as in the wild type plants treated with epigallocatechin gallate(EGCG),an inhibitor of pectin methylesterases,suggesting cell wall integrity as a critical factor that determines the expression pattern of stress-responsive genes.Production of reactive oxygen species(ROS)is constitutively increased in the lrx345 and fer-4mutants,and inhibition of ROS accumulation suppresses the salt-hypersensitivity of these mutants.Together,our work provides strong evidence that the LRX3/4/5-RALF22/23-FER module controls plant growth and salt stress responses by regulating hormonal homeostasis and ROS accumulation. | Chunzhao Zhao Wei Jiang Omar Zayed Xin Liu Kai Tang Wenfeng Nie Yali Li Shaojun Xie Yuan Li Tiandan Long Linlin Liu Yingfang Zhu Yang Zhao Jian-Kang Zhu | 2021 | National Science Review2021，8，1： | 3 |
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