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| 1 | The Genome of Artemisia annua Provides Insight into the Evolution of Asteraceae Family and Artemisinin Biosynthesis显示文摘Artemisia annua,通常已知的同样香甜的苦恼或 Qinghao,是到中国的一个灌木土著人并且长被用于药用的目的。A。annua 现在作为有势力抗疟药混合物的唯一的生来的来源全球性被栽培, artemisinin。这里,我们报导 A 的 1.74-gigabase 染色体的一个高质量的草稿集会。annua,高度异质接合,富于重复定序,并且包含 63 ? 226 编码蛋白质的基因,在定序的植物种类之中的最大的数字之一。我们发现了那,作为在 Asteraceae 的一些定序的染色体之一, A。annua 染色体包含对这大被子植物 clade 特定的很多基因。尤其是,扩大和编码涉及萜烯生合成的酶的基因的功能的多样化与 artemisinin biosynthetic 小径的进化一致。我们进一步由介绍那 A 的 transcriptome 揭示了。annua 发展了复杂 transcriptional 规章的网络位于 \O 下面 artemisinin 生合成。把转基因的 A 基于我们产生了的全面 genomic 和 transcriptomic 分析。artemisinin 高级的生产的 annua 线,它现在为大规模生产准备好了并且将从而帮助遇见增加 artemisinin 的全球需求的挑战。 | Qian Shen Lida Zhang Zhihua Liao Shengyue Wang Tingxiang Yan Pu Shi Meng Liu Xueqing Fu Qifang Pan Yuliang Wang Zongyou Lv Xu Lu Fangyuan Zhang Weimin Jiang Yanan Ma Minghui Chen Xiaolong Hao Ling Li Yueli Tang Gang Lv Yan Zhou Xiaofen Sun Peter E. Brodelius Jocelyn K.C. Rose Kexuan Tang | 2018 | Molecular Plant2018，11，6： | 47 |
| 2 | Increased Vitamin C Content Accompanied by an Enhanced Recycling Pathway Confers Oxidative Stress Tolerance in Arabidopsis显示文摘Vitamin C(L-ascorbic acid,AsA) has important antioxidant and metabolic functions in both plants and animals.Once used,ascorbic acid can be regenerated from its oxidized form in a reaction catalyzed by dehydroascorbate reductase(DHAR,EC 1.8.5.1).To analyze the physiological role of DHAR catalyzing the reduction of DHA to ascorbate in environmental stress adaptation,we examined whether increasing the level of AsA through enhanced AsA recycling would limit the deleterious effects of oxidative stress.A chimeric construct consisting of the double CaMV35S promoter fused to the Myc-dhar gene was introduced into Arabidopsis thaliana.Transgenic plants were biochemically characterized and tested for responses to oxidative stress.Western blot indicated that the dhar-transgene was successfully expressed.In homozygous T4 transgenic seedlings,DHAR overexpression was increased up to 1.5 to 5.4 fold,which enhanced foliar ascorbic acid levels 2-to 4.25-fold and ratio of AsA/DHA about 3-to 16-fold relative to wild type.In addition,the level of glutathione,the reductant used by DHAR,also increased as did its redox state.When whole plants were treated with high light and high temperature stress or in vitro leaf discs were subjected to 10 μM paraquat,transgenic plants showed a larger AsA pool size,lower membrane damage,and a higher level of chlorophyll compared with controls.These data suggested that increasing the plant vitamin C content through enhanced ascorbate recycling could limit the deleterious effects of environmental oxidative stress. | zinan wang Ying Xiao Wansheng Chen Kexuan Tang Lei Zhang | 2010 | Journal of Integrative Plant Biology2010，52，4： | 16 |
| 3 | The transcription factors TLR1 and TLR2 negatively regulate trichome density and artemisinin levels in Artemisia annua显示文摘The important antimalarial drug artemisinin is biosynthesized and stored in Artemisia annua glandular trichomes and the artemisinin content correlates with trichome density;however,the factors affecting trichome development are largely unknown.Here,we demonstrate that the A.annua R2R3 MYB transcription factor TrichomeLess Regulator 1(TLR1)negatively regulates trichome development.In A.annua,TLR1 overexpression lines had 44.7%–64.0%lower trichome density and 11.5%–49.4%lower artemisinin contents and TLR1-RNAi lines had 33%–93.3%higher trichome density and 32.2%–84.0%higher artemisinin contents compared with non-transgenic controls.TLR1 also negatively regulates the expression of anthocyanin biosynthetic pathway genes in A.annua.When heterologously expressed in Arabidopsis thaliana,TLR1 interacts with GLABROUS3a,positive regulator of trichome development,and represses trichome development.Yeast two-hybrid and pull-down assays indicated that TLR1 interacts with the WUSCHEL homeobox(WOX)protein AaWOX1,which interacts with the LEAFY-like transcription factor TLR2.TLR2 overexpression in Arabidopsis and A.annua showed that TLR2 reduces trichome development by reducing gibberellin levels.Furthermore,artemisinin contents were 19%–43%lower in TLR2-overexpressing A.annua plants compared to controls.These data indicate that TLR1 and TLR2 negatively regulate trichome density by lowering gibberellin levels and may enable approaches to enhance artemisinin yields. | Zongyou Lv JinXing Li Shi Qiu Fei Qi Hang Su Qitao Bu Rui Jiang Kexuan Tang Lei Zhang Wansheng Chen | 2022 | Journal of Integrative Plant Biology2022，64，6： | 2 |
| 4 | AaWRKY17, a positive regulator of artemisinin biosynthesis, is involved in resistance to Pseudomonas syringae in Artemisia annua显示文摘Artemisia annua,a traditional Chinese medicinal plant,remains the only plant source for artemisinin production,yet few genes have been identified to be involved in both the response to biotic stresses,such as pathogens,and artemisinin biosynthesis.Here,we isolated and identified the WRKY transcription factor(TF)AaWRKY17,which could significantly increase the artemisinin content and resistance to Pseudomonas syringae in A.annua.Yeast one-hybrid(Y1H),dual-luciferase(dual-LUC),and electrophoretic mobility shift assay(EMSA)results showed that AaWRKY17 directly bound to the W-box motifs in the promoter region of the artemisinin biosynthetic pathway gene amorpha-4,11-diene synthase(ADS)and promoted its expression.Real-time quantitative PCR(RT-qPCR)analysis revealed that the transcript levels of two defense marker genes,Pathogenesis-Related 5(PR5)and NDR1/HIN1-LIKE 10(NHL10),were greatly increased in AaWRKY17-overexpressing transgenic A.annua plants.Additionally,overexpression of AaWRKY17 in A.annua resulted in decreased susceptibility to P.syringae.These results indicated that AaWRKY17 acted as a positive regulator in response to P.syringae infection.Together,our findings demonstrated that the novel WRKY transcription factor AaWRKY17 could potentially be used in transgenic breeding to improve the content of artemisinin and pathogen tolerance in A.annua. | Tiantian Chen Yongpeng Li Lihui Xie Xiaolong Hao Hang Liu Wei Qin Chen Wang Xin Yan Kuanyu Wu-Zhang Xinghao Yao Bowen Peng Yaojie Zhang Xueqing Fu Ling Li Kexuan Tang | 2021 | Horticulture Research2021，8，1： | 2 |
| 5 | Asymmetric interfaces and high-TC ferromagnetic phase in La_(0.67)Ca_(0.33)MnO_(3)/SrRuO_(3)superlattices显示文摘Interfacial magnetism in functional oxide heterostructures not only exhibits intriguing physical phenomena but also implies great potential for device applications.In these systems,interfacial structural and electronic reconstructions are essential for improving the stability and tunability of the magnetic properties.In this work,we constructed ultra-thinLa_(0.67)Ca_(0.33)MnO_(3)/SrRuO_(3)(SRO)layers into superlattices,which exhibited a robust ferromagnetic phase.The high Curie temperature(TC)reaches 291 K,more than 30 K higher than that of bulk LCMO.We found that the LCMO/SRO superlattices consisted of atomically-sharp and asymmetric heterointerfaces.Such a unique interface structure can trigger a sizable charge transfer as well as a ferroelectric-like polar distortion.These two interfacial effects cooperatively stabilized the high-T_(C)ferromagnetic phase.Our results could pave a promising approach towards effective control of interfacial magnetism and new designs of oxide-based spintronic devices. | Lili Qu Da Lan Liang Si Chao Ma Shasha Wang Liqiang Xu Kexuan Zhang Feng Jin Zixun Zhang Enda Hua Binbin Chen Guanyin Gao Feng Chen Haifeng Du Karsten Held Lingfei Wang Wenbin Wu | 2021 | Nano Research2021，14，10： | 1 |
| 6 | Realizing the highly reversible Zn^(2+)and Na^(+) dual ions storage in high-crystallinity nickel hexacyanoferrate microcubes for aqueous zinc-ion batteries显示文摘Prussian blue analogues(PBAs)with the 3D open framework are regarded as promising cathode candidates for aqueous Zinc ion batteries(ZIBs).Among various PBAs,nickel hexacyanoferrate(NiHCF)has attracted considerable attention because of its high operating voltage and economic merit.However,the cyclability of NiHCF is unsatisfactory due to poor structural stability during Zn^(2+) ions insertion/deinsertion.Moreover,the ion storage mechanism of NiHCF in aqueous electrolytes has not been fully revealed yet.Herein,high-crystallinity NiHCF(HC-NiHCF)microcubes with improved structural stability and larger crystal plane spacing are synthesized.For the first time,highly reversible Zn2+ions and Na+ions co-insertion/extraction are achieved for the HC-NiHCF microcubes in mixed aqueous electrolyte,as evidenced by various observations including two separated discharge plateaus and sequential changes of Na 1s and Zn 2p signals in ex-situ X-ray photoelectron spectroscopy(XPS).As a result,a high specific capacity of 73.9 mAh g^(−1) is obtained for the HC-NiHCF microcubes at 0.1 A g−1,combined with enhanced cycle stability(75%vs.16.4%)over 1000 cycles at 2 A g^(−1).The reversible Zn^(2+) ions and Na+ions co-insertion in HC-NiHCF microcubes reveals a new ion storage mechanism of Ni-based PBAs in aqueous electrolytes. | Kexuan Wang Zhu Xu Heng Li Huibo Wang Mingzheng Ge Jilei Liu Shengwen Li Zekun Hu Mengyu Zhu Yanyan Zhang Yuxin Tang Shi Chen | 2023 | Journal of Materials Science & Technology2023，，33： | 1 |
| 7 | scNanoATAC-seq:a long-read single-cell ATAC sequencing method to detect chromatin accessibility and genetic variants simultaneously within an individual cell显示文摘Dear Editor,The accessible chromatin regions were enriched in regulatory elements in human genome and many genetic variations associated with human diseases were identified within them.1 Single-cell assay for transposase-accessible chromatin using sequencing(scATAC-seq)on the next-generation sequencing(NGS)platform is a well-established method to detect open chromatin regions within an individual cell.2 However,there remain challenges in detection of large-scale structural variations(SVs,including insertions,deletions,duplications,inversions and translocations)and haplotype phasing from scATAC-seq data,which can be well resolved by third-generation sequencing(TGS)platform-based single-molecule long-read sequencing.To integrate advantages of long-read sequencing into scATAC-seq,we developed single-cell assay for transposase‐accessible chromatin on Nanopore sequencing platform(scNanoATAC-seq),a platebased scATAC-seq method suitable for TGS platform(Supplementary information,Fig.S1). | Yuqiong Hu Zhenhuan Jiang Kexuan Chen Zhangxian Zhou Xin Zhou Yan Wang Jingwei Yang Bo Zhang Lu Wen Fuchou Tang | 2023 | Cell Research2023，33，1： | 1 |
| 8 | Highly Enantioselective Hydrogenation of β,β‐Disubstituted Nitroalkenes显示文摘 | Shengkun Li Kexuan Huang Bonan Cao Jiwen Zhang Wenjun Wu Xumu Zhang | 2012 | Angew Chem Int Ed2012，，34： | 1 |
| 9 | Identification and expression profile of GbAGL2, a C-class gene from G ossypium barbadense显示文摘 | Xiang Liu Kaijing Zuo Fei Zhang Ying Li Jieting Xu Lida Zhang Xiaofen Sun Kexuan Tang | 2009 | Journal of Biosciences2009，，6： | 1 |
| 10 | Molecular insights into AabZIP1-mediated regulation on artemisinin biosynthesis and drought tolerance in Artemisia annua显示文摘Artemisia annua is the main natural source of artemisinin production.In A.annua,extended drought stress severely reduces its biomass and artemisinin production while short-term water-withholding or abscisic acid(ABA)treatment can increase artemisinin biosynthesis.ABA-responsive transcription factor AabZIP1 and JA signaling AaMYC2 have been shown in separate studies to promote artemisinin production by targeting several artemisinin biosynthesis genes.Here,we found AabZIP1 promote the expression of multiple artemisinin biosynthesis genes including AaDBR2 and AaALDH1,which AabZIP1 does not directly activate.Subsequently,it was found that AabZIP1 up-regulates AaMYC2expression through direct binding to its promoter,and that AaMYC2 binds to the promoter of AaALDH1to activate its transcription.In addition,AabZIP1 directly transactivates wax biosynthesis genes AaCER1and AaCYP86A1.The biosynthesis of artemisinin and cuticular wax and the tolerance of drought stress were significantly increased by AabZIP1 overexpression,whereas they were significantly decreased in RNAi-AabZIP1 plants.Collectively,we have uncovered the AabZIP1-AaMYC2 transcriptional module as a point of cross-talk between ABA and JA signaling in artemisinin biosynthesis,which may have general implications.We have also identified AabZIP1 as a promising candidate gene for the development of A.annua plants with high artemisinin content and drought tolerance in metabolic engineering breeding. | Guoping Shu Yueli Tang Mingyuan Yuan Ning Wei Fangyuan Zhang Chunxian Yang Xiaozhong Lan Min Chen Kexuan Tang Lien Xiang Zhihua Liao | 2022 | Acta Pharmaceutica Sinica B2022，12，3： | 1 |
| 11 | Two-photon 3D printed spring-based Fabry-Pérot cavity resonator for acoustic wave detection and imaging显示文摘Optical fiber microresonators have attracted considerable interest for acoustic detection because of their compact size and high optical quality.Here,we have proposed,designed,and fabricated a spring-based Fabry-Pérot cavity microresonator for highly sensitive acoustic detection.We observed two resonator vibration modes:one relating to the spring vibration state and the other determined by the point-clamped circular plate vibration mode.We found that the vibration modes can be coupled and optimized by changing the structure size.The proposed resonator is directly 3D printed on an optical fiber tip through two-photon polymerization and is used for acoustic detection and imaging.The experiments show that the device exhibits a high sensitivity and low noise equivalent acoustic signal level of 2.39 mPa∕Hz^(1∕2)at 75 kHz that can detect weak acoustic waves,which can be used for underwater object imaging.The results demonstrate that the proposed work has great potential in acoustic detection and biomedical imaging applications. | HEMING WEI ZHANGLI WU KEXUAN SUN HAIYAN ZHANG CHEN WANG KEMIN WANG TIAN YANG FUFEI PANG XIAOBEI ZHANG TINGYUN WANG SRIDHAR KRISHNASWAMY | 2023 | Photonics Research2023，11，5： | 0 |
| 12 | Causal reasoning in typical computer vision tasks显示文摘Deep learning has revolutionized the field of artificial intelligence.Based on the statistical correlations uncovered by deep learning-based methods,computer vision tasks,such as autonomous driving and robotics,are growing rapidly.Despite being the basis of deep learning,such correlation strongly depends on the distribution of the original data and is susceptible to uncontrolled factors.Without the guidance of prior knowledge,statistical correlations alone cannot correctly reflect the essential causal relations and may even introduce spurious correlations.As a result,researchers are now trying to enhance deep learningbased methods with causal theory.Causal theory can model the intrinsic causal structure unaffected by data bias and effectively avoids spurious correlations.This paper aims to comprehensively review the existing causal methods in typical vision and visionlanguage tasks such as semantic segmentation,object detection,and image captioning.The advantages of causality and the approaches for building causal paradigms will be summarized.Future roadmaps are also proposed,including facilitating the development of causal theory and its application in other complex scenarios and systems. | ZHANG KeXuan SUN QiYu ZHAO ChaoQiang TANG Yang | 2024 | Science China(Technological Sciences)2024，67，1： | 0 |
| 13 | Nature-inspired materials and designs for flexible lithium-ion batteries显示文摘Flexible lithium-ion batteries(FLBs)are of critical importance to the seamless power supply of flexible and wearable electronic devices.However,the simultaneous acquirements of mechanical deformability and high energy density remain a major challenge for FLBs.Through billions of years of evolutions,many plants and animals have developed unique compositional and structural characteristics,which enable them to have both high mechanical deformability and robustness to cope with the complex and stressful environment.Inspired by nature,many new materials and designs emerge recently to achieve mechanically flexible and high storage capacity of lithiumion batteries at the same time.Here,we summarize these novel FLBs inspired by natural and biological materials and designs.We first give a brief introduction to the fundamentals and challenges of FLBs.Then,we highlight the latest achievements based on nature inspiration,including fiber-shaped FLBs,origami and kirigami-derived FLBs,and the nature-inspired structural designs in FLBs.Finally,we discuss the current status,remaining challenges,and future opportunities for the development of FLBs.This concise yet focused review highlights current inspirations in FLBs and wishes to broaden our view of FLB materials and designs,which can be directly“borrowed”from nature. | Heng Li Huibo Wang Dan Chan Zhu Xu Kexuan Wang Mingzheng Ge Yanyan Zhang Shi Chen Yuxin Tang | 2022 | Carbon Energy2022，4，5： | 0 |
| 14 | Association of variants in the KIF1A gene with amyotrophic lateral sclerosis显示文摘Background:Amyotrophic lateral sclerosis(ALS)is a devastating progressive neurodegenerative disease that affects neurons in the central nervous system and the spinal cord.As in many other neurodegenerative disorders,the genetic risk factors and pathogenesis of ALS involve dysregulation of cytoskeleton and neuronal transport.Notably,sen-sory and motor neuron diseases such as hereditary sensory and autonomic neuropathy type 2(HSAN2)and spastic paraplegia 30(SPG30)share several causative genes with ALS,as well as having common clinical phenotypes.KIF1A encodes a kinesin 3 motor that transports presynaptic vesicle precursors(SVPs)and dense core vesicles and has been reported as a causative gene for HSAN2 and SPG30.Methods:Here,we analyzed whole-exome sequencing data from 941 patients with ALS to investigate the genetic association of KIF1A with ALS.Results:We identified rare damage variants(RDVs)in the KIF1A gene associated with ALS and delineated the clini-cal characteristics of ALS patients with KIF1A RDVs.Clinically,these patients tended to exhibit sensory disturbance.Interestingly,the majority of these variants are located at the C-terminal cargo-binding region of the KIF1A protein.Functional examination revealed that the ALS-associated KIF1A variants located in the C-terminal region preferentially enhanced the binding of SVPs containing RAB3A,VAMP2,and synaptophysin.Expression of several disease-related KIF1A mutants in cultured mouse cortical neurons led to enhanced colocalization of RAB3A or VAMP2 with the KIF1A motor.Conclusions:Our study highlighted the importance of KIF1A motor-mediated transport in the pathogenesis of ALS,indicating KIF1A as an important player in the oligogenic scenario of ALS. | Panlin Liao Yanchun Yuan Zhen Liu Xiaorong Hou Wanzhen Li Jin Wen Kexuan Zhang Bin Jiao Lu Shen Hong Jiang Jifeng Guo Beisha Tang Zhuohua Zhang Zhonghua Hu Junling Wang | 2022 | Translational Neurodegeneration2022，11，1： | 0 |
| 15 | A self-guidance biological hybrid drug delivery system driven by anaerobes to inhibit the proliferation and metastasis of colon cancer显示文摘Colorectal cancer is often accompanied by multiple organ metastasis.Anaerobic Bifidobacterium Infantis(BI)bacterial can selectively grow in hypoxic colorectal tumor microenvironment(TME),to own the natural advantage of preferentially colorectal tumor targeting.Herein,a self-guidance biological hybrid drug delivery system(BI-ES-Fe Alg/DOX)based on BI was constructed to inhibit the proliferation and metastasis of colon cancer.Results demonstrated that BI-ES-Fe Alg/DOX could overcome physical barriers to target and accumulate in colon tumor tissues.Then DOX was released to kill tumor cells along with the phase transition(solid to liquid)of Fe Alg hydrogel,due to Fe3+was reduced to Fe^(2+)by intracellular GSH.Meanwhile,BI-ES selectively colonized into tumors and expressed endostatin(ES)protein to down-regulate VEGF and b FGF expression,exerting anti-angiogenic effect.Moreover,Fe Alg catalyzed H_(2)O_(2)in the local tumor to generate cytotoxic·OH,further enhancing the antitumor effect.The pharmacodynamic result in AOM/DSS model proved that BI-ES-Fe Alg/DOX had the best therapeutic effect,with the final V/V0of 2.19±0.57,which was significantly lower than the other groups.Meanwhile,on CT-26tumor-bearing model,it also showed an outstanding anti-tumor effect with inhibition rate of 82.12%±3.08%.In addition,lung metastases decreased significantly in tumor metastasis model after BI-ES-Fe Alg/DOX treatment. | Huijuan Zhang Yaping Wang Mengting Li Kexuan Cao Zijun Qi Ling Zhu Zhenzhong Zhang Lin Hou | 2022 | Asian Journal of Pharmaceutical Sciences2022，17，6： | 0 |
| 16 | PINK1-mediated Drp1^(S616) phosphorylation modulates synaptic development and plasticity via promoting mitochondrial fission显示文摘Dynamic change of mitochondrial morphology and distribution along neuronal branches are essential for neural circuitry formation and synaptic efficacy.However,the underlying mechanism remains elusive.We show here that Pink1 knockout(KO)mice display defective dendritic spine maturation,reduced axonal synaptic vesicles,abnormal synaptic connection,and attenuated long-term synaptic potentiation(LTP).Drp1 activation via ^(S616) phosphorylation rescues deficits of spine maturation in Pink1 KO neurons. | Qingtao Gao Runyi Tian Hailong Han Jesse Slone Caifang Wang Xiao Ke Tongmei Zhang Xiangyu Li Yuhong He Panlin Liao Fang Wang Ye Chen Shiqing Fu Kexuan Zhang Fangfang Zeng Yingxuan Yang Zhuo Li Jieqiong Tan Jiada Li Youming Lu Taosheng Huang Zhonghua Hu Zhuohua Zhang | 2022 | Signal Transduction and Targeted Therapy2022，7，5： | 0 |
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