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| 1 | Y-chromosome evidence for no independent origin of modern human in China显示文摘East Asia is one of the few regions in the world where a large number of human fossils have been unearthed. The continuity of hominid fossils in East Asia, particularly in China has been presented as strong evidence supporting an independent origin of modern humans in this area. To search for such evidence of a possible independent origin of modern humans in China, a total of 9988 male individuals were sampled across China. Three Y-chromosome biallelic markers (M89, M130 and YAP), which were located at the non-re-combinant region of Y-chromosome, were typed among the samples. Our result showed that all the individuals carry a mutation at one of the three loci. The three mutations (M89T, M130T, YAP+) coalesce to another mutation (M168T), which was originally derived from Africa about 31000 to 79000 years ago. In other words, all Y-chromosome samples from China, with no exception, were originally derived from a lineage of African origin. Hence, we conclude that even a very minor contribution of in situ hominid origin in China cannot be supported by the Y-chromosome evidence. | KE Yuehai SU Bing LI Hongyu CHEN Lifeng QI Chunjian GUO Xinjun HUANG Wei JIN Jianzhong LU Daru JIN Li | 2001 | Chinese Science Bulletin2001，46，11： | 7 |
| 2 | Effects of CIITA antisense RNA on the expression of HLA class Ⅱ molecules显示文摘To study the effect of the major histocompatibility complex class Ⅱ (MHC Ⅱ ) transactivator (CIITA) antisense RNA on the expression of the human leukemia (HLA) class Ⅱ molecules, 5’ end cDNA sequence of CIITA gene was cloned, and antisense RNA expression vector pcDNA-Ⅱ was constructed. HeLa cells transfected with pcDNA-Ⅱ and pcDNA3 were induced by IFN-γ for 3 d. The expression of HLA class Ⅱ molecules on HeLa/pcDNA-Ⅱ cells was significantly decreased, while it has no effect on the expression of HLA class Ⅰ molecules. This result suggests that the CIITA antisense RNA can inhibit the expression of HLA class Ⅱ molecules in HeLa cells. It also implies a promising approach to generate immune tolerance in qraft transplantation. | ZHOU Caihong, LU Daru, ZHU Qiquan, QIU Xinfang & XUE JinglunInstitute of Genetics, Fudan University, Shanghai 200433, China | 2000 | Chinese Science Bulletin2000，45，22： | 2 |
| 3 | Efficient transfer and expression of human clotting factor IX cDNA in neonatal hemophilia B mice mediated by VSV-G pseudotyped retrovi-rus显示文摘The feasibility of in vivo gene therapy for hemophilia B by VSV-G pseudotyped retroviral vector was introduced. The novel packaging cell line 293GPG was used to produce VSV-G/G1NaBAIX pseudotyped virus with the highest liters up to 8.5×108cfu .mL-1. In contrast to the conventional retrovirus, VSV-G pseudotyped virus was more resistant to inactivation by serum complements (P<0.001). Our results also demonstrated that VSV-G pseudotyped virus was more stable in neonatal mice serum than in adult mice serum (P<0.01). After intraperitoneal injection of different doses of virus, hFIX antigen was detected and lasted for more than 120 d, the highest level reached (72.5+6.1) ng- mL11. Moreover, the functional activity was improved to some extent in all hFIX-treated mice, the most remarkable improvement was observed in the mice treated with higher dose of virus whose clotting activity increased to (3.4±1.5)% and APTT (activated partial thromboplastin time) reduced to (43.2±7.2) s. The anti-hFIX antibody was | WANG Hongwei YE Chenbo CHEN Li WANG Xuefeng QIU Xinfang XUE Jinglun LU Daru | 2001 | Chinese Science Bulletin2001，46，18： | 2 |
| 4 | Expression of biologically active human clotting factor Ⅸ(hFⅨ) in the mammary gland of transgenic mice显示文摘The DNA of human factor Ⅸ (hFⅨ) gene vector pMCⅨm, which had been proven to be able to express in in vitro and living cells, was introduced into 586 zygotes of Kunming White Mice by positive pressure microinjection technique with manual operation. The 499 survival embryos after microinjection were then transferred into pseudopregnant recipient mice and 216 F 0 pups were born. The analysis of PCR and Southern blot hybridization showed that, of the 216, 6 (2 females and 4 males) were integrated with foreign DNA in their genomes, giving an integration frequency of 3% (6/216). Two F\-0 female transgenic mice could express hFⅨ protein in their milk and the content was over 100 ng/mL as measured with ELISA. The biological activities of hFⅨ in the milk of two F\-0 mice were 44 67% and 79 43%, respectively. | HUANG Ying 1, ZHANG Kezhong 2, HUANG Wenying 1, LU Daru 2, HUANG Ying 1, MA Zhanlu 1, REN Zhaorui 1, QIU Xinfang 2, XUE Jinglun 2, ZENG Yitao 1 and HUANG Shuzhen 1* 1. Shanghai Institute of Medical Genetics, Shanghai Children’s Hospita | 1998 | Chinese Science Bulletin1998，43，15： | 2 |
| 5 | Polymorphisms of the vascular endothelial growth factor A gene and susceptibility to sporadic brain arteriovenous malformation in a Chinese population显示文摘 | Hongyan Chen Yuxiang Gu Wenting Wu Dan Chen Peiliang Li Weiwei Fan Daru Lu Fan Zhao Nidan Qiao Huijia Qiu Chaowei Fu Ying Mao Yao Zhao | 2010 | Journal of Clinical Neuroscience2010，，4： | 1 |
| 6 | Multiple origins of Tibetan Y chromosomes显示文摘 | Yaping Qian Binzhi Qian Bing Su Jiankun Yu Yuehai Ke Zhengtao Chu Lei Shi Daru Lu Jiayou Chu Li Jin | 2000 | Human Genetics2000，，4： | 1 |
| 7 | Spieial chromosomal translocation and therapy-related leukemia induced by bimolane therapy for psoriasis显示文摘 | Yongquan Xue Daru Lu Yu Guo | 1992 | Leulemia Research1992，16，： | 1 |
| 8 | Low-loss hybrid plasmonic waveguide based on metal ridge and semiconductor nanowire显示文摘 | Lu Qijing Chen Daru Wu Genzhu | 2013 | Optics Communications2013，289，： | 1 |
| 9 | PCR-RFLP analysis of cytochrome b gene does not support Coilia ectenes taihuensis being a subspecies of Coilia ectenes显示文摘 | Chen Qiqun Lu Daru | 2005 | Journal of Genetic2005，84，3： | 1 |
| 10 | Multiple origins of Tibetan Y chromosomes显示文摘 | Yaping Qian Binzhi Qian Bing Su Jiankun Yu Yuehai Ke Zhengtao Chu Lei Shi Daru Lu Jiayou Chu Li Jin | 2000 | Human Genetics2000，，4： | 1 |
| 11 | 本校近期发表IF≥4.0的SCI论文摘要(英文)——Genetic Variants on Chromosome 15q25 Associated with Lung Cancer Risk in Chinese Populations显示文摘 | Hu Zhibin Jin Guangfu Liang Jie Zhang Mingfeng Shen Hongbing Wu Chen Yu Dianke Huang Liming Tan Wen Lin Dongxin Guo Huan Wu Tangchun Qian Ji Lu Daru | 2009 | 南京医科大学学报(自然科学版)2009，29，9： | 1 |
| 12 | Human clotting factor Ⅸ (hF Ⅸ ) secretion in goat milk after direct transfer with hF Ⅸ minigene into mammary gland by using retroviral vectors显示文摘THE mammary gland bioreactor system of lactating animal, established by germ-linemicroinjection and embryo transplantation, has been one of the most exciting projects in thefield of biotechnology. However, this system is costly, laborious and tim-cosuming. In 1994,Johanna et al. reported that secretion of a foreign protein, human growth hormone (hGH),in milk had been achieved after direct introduction of the cDNA into the mammary gland ofgoats by replication-defective retroviral vector. In that work, the in vivo expression of | ZHANG Kezhong, WANG Hongwei, BAO Yun, LU Daru,XUE Jinglun (J. L. Hsueh), QIU Xinfang, HUANG Shuzhen,HUANG Ying, LI Bingyan, LI Hua and ZENG Yitao1. Institute of Genetics, Fudan University, Shanghai 200433, China 2 Shanghai Children’s Hospital, Shanghai Institute of Medical Genetics, Shanghai 200040, China | 1997 | Chinese Science Bulletin1997，42，15： | 1 |
| 13 | Y chromosome haplotypes reveal prehistorical migrations to the Himalayas显示文摘 | Bing Su Chunjie Xiao Ranjan Deka Mark T. Seielstad Daoroong Kangwanpong Junhua Xiao Daru Lu Peter Underhill Luca Cavalli-Sforza Ranajit Chakraborty Li Jin | 2000 | Human Genetics2000，，6： | 1 |
| 14 | Overexpression of SLC7A7 predicts poor progression-free and overall survival in patients with glioblastoma显示文摘 | Songhua Fan Delong Meng Tao Xu Yuanyuan Chen Jingkun Wang Xiaoying Li Hongyan Chen Daru Lu Juxiang Chen Qing Lan | 2013 | Medical Oncology2013，，1： | 1 |
| 15 | Upregulation of miR-15b in NAFLD models and in the serum of patients with fatty liver disease显示文摘 | Yuhao Zhang Xixiang Cheng Zhiqiang Lu Jingkun Wang Hongyan Chen Weiwei Fan Xin Gao Daru Lu | 2012 | Diabetes Research and Clinical Practice2012，，： | 1 |
| 16 | pH-sensitive poly(glutamic acid) grafted mesoporous silica nanoparticles for drug delivery显示文摘 | Jin Zheng Xuejiao Tian Yangfei Sun Daru Lu Wuli Yang | 2013 | International Journal of Pharmaceutics (-)2013，，1： | 1 |
| 17 | miR-1205/DNAJB1 reverses docetaxel chemoresistance in human triple negative breast carcinoma cells via regulation of mutp53/TAp63 signaling显示文摘Chemoresistance is the major cause of therapeutic failure in human triple negative breast carcinoma(TNBC).Docetaxel(DOC),a first-line therapeutic drug in TNBC treatment,is limited for long-term use due to the development of chemoresistance.Thus,overcoming chemoresistance of DOC remains an important challenge to improve patient’s outcome of TNBC.In this study,we aimed to investigate the molecular mechanism behind DOC chemoresistance and the possible therapeutic effects of miRNAs.Utilizing qRT-PCR analysis,we discovered that miR-1205 is gradually downregulated in human triple negative breast carcinoma MDA-MB-231 and docetaxel-resistant MDA-MB-231(MDA-MB-231/DOC)cells compared with Hs 578Bst normal human breast fibroblasts.Cell viability,cell cycle and apoptosis assays in MDA-MB-231/DOC cells indicated that miR-1205 overexpression enhances docetaxel sensitivity by reducing cell viability as well as inducing G2/M cell cycle arrest and cell apoptosis.Western blot analysis,dual-luciferase reporter assay,co-immunoprecipitation assay and chromatin immunoprecipitation assay revealed that miR-1205 overexpression disrupts the stable complex formation of DNAJB1,mutp53 and TAp63 by directly reducing DNAJB1 expression,which abates the sequestrating effect of mutp53 on TAp63,thereby leading to the enhanced DOC sensitivity in MDA-MB-231/DOC cells.Our findings demonstrate the role of the miR-1205/DNAJB1 axis in the docetaxel resistance of TNBC,which may offer a promising therapeutic approach to resolve docetaxel resistance in TNBC. | Yongxiang Yin Jinqiu Zhang Tao Ma Daozhen Chen Daru Lu | 2022 | Acta Biochimica et Biophysica Sinica2022，54，1： | 1 |
| 18 | Genetic evidence supports demic diffusion of Han culture显示文摘 | LI Hui LU Daru SONG Xiufeng ZHANG Feng HE Yungang LI Feng GAO Yang MAO Xianyun ZHANG Liang QIAN Ji TAN Jingze JIN Jianzhong HUANG Wei DEKA Ranjan SU Bing CHAKRABORTY Ranajit JIN Li | | NATURE0，2004，： | 1 |
| 19 | Prudently conduct the engineering and synthesis of the SARS-CoV-2 virus显示文摘In response to the outbreak of COVID-19 that has been sweeping the world,scientists reconstructed the SARSCoV-2 rapidly using a synthetic genomics platform,in order to accelerate therapeutics and vaccine development.However,given the dual-use nature of this technology,there exists a high biosecurity risk.This paper points out the potential risks of the engineering SARS-CoV-2 virus and puts forward 6 questions to this work.The authors emphasize that the two basic values of safety/security and intellectual freedom of research must be considered evenly.From the perspective of responsible development of biotechnology,this paper calls for a careful assessment to the risks of the technology,replacing risky technologies with safe ones.The risks of publication also need to be strictly assessed.The authors believe,in addition to enhancing the“self-government”and self-discipline of scientists and scientific communities,government supervision must be reinforced,laws and regulations should be improved,and global regulation framework ought to be constructed. | Peng Gao Shiwen Ma Daru Lu Carl Mitcham Yijia Jing Guoyu Wang | 2020 | Synthetic and Systems Biotechnology2020，5，2： | 0 |
| 20 | Novel rAAV production system with low contamina-tion of helper virus显示文摘The Cre-loxP system was introduced in theproduction of recombinant AAV (rAAV). Defective adenovi-rus AdLC was used as the helper virus. While doing themass production of recombinant AAV carrying EGFP orhuman clotting factor Ⅸ (hFⅨ) gene, the generation ofhelper virus was significantly limited, it increased the sim-plicity of rAAV purification. The results from in vivo study demonstrated the superiority of this method. This systemprovides a novel approach for the application of rAAV sys-tem in gene therapy. | CHEN Li, CHEN Haoming, LI Ge, LU Daru & XUE Jinglun State Key Laboratory of Genetic Engineering, Institute of Genetics,School of Life Sciences, Fudan University, Shanghai 200433, China Correspondence should be addressed to Xue Jinglun (e-mail: jlxue@ fudan.ac.cn) | 2003 | Chinese Science Bulletin2003，48，5： | 0 |
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