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| 1 | Rebamipide suppresses diclofenac-induced intestinal permeability via mitochondrial protection in mice显示文摘AIM: To investigate the protective effect and mechanism of rebamipide on small intestinal permeability induced by diclofenac in mice. METHODS: Diclofenac (2.5 mg/kg) was administered once daily for 3 d orally. A control group received the vehicle by gavage. Rebamipide (100 mg/kg, 200 mg/kg, 400 mg/kg) was administered intragastrically once a day for 3 d 4 h after diclofenac administration. Intestinal permeability was evaluated by Evans blue and the FITC-dextran method. The ultrastructure of the mucosal barrier was evaluated by transmission electron microscopy (TEM). Mitochondrial function including mitochondrial swelling, mitochondrial membrane potential, mitochondrial nicotinamide adenine dinucleotide-reduced (NADH) levels, succinate dehydrogenase (SDH) and ATPase activities were measured. Small intestinal mucosa was collected for assessment of malondialdehyde (MDA) content and myeloperoxidase (MPO) activity. RESULTS: Compared with the control group, intestinal permeability was significantly increased in the diclofenac group, which was accompanied by broken tight junctions, and significant increases in MDA content and MPO activity. Rebamipide significantly reduced intestinal permeability, improved inter-cellular tight junctions, and was associated with decreases in intestinal MDA content and MPO activity. At the mitochondrial level, rebamipide increased SDH and ATPase activities, NADH level and decreased mitochondrial swelling. CONCLUSION: Increased intestinal permeability induced by diclofenac can be attenuated by rebamipide, which partially contributed to the protection of mitochondrial function. | Lei Diao Qiao Mei Jian-Ming Xu Xiao-Chang Liu Jing Hu Juan Jin Qiang Yao Mo-Li Chen | 2012 | World Journal of Gastroenterology2012，18，10： | 8 |
| 2 | 桑螵蛸脂类提取物对铜绿假单胞菌的抗菌活性和抗生物膜作用(英文)显示文摘目的:探究桑螵蛸脂类提取物的成分及对铜绿假单胞菌的抗菌和抗生物膜作用。创新点:中国传统中药桑螵蛸一直广泛应用于肾病的治疗,在抗菌领域未见报道,本实验首次证明桑螵蛸脂类提取物对铜绿假单胞菌有明显的抗菌和抗生物膜作用。方法:采用气相色谱-质谱联用技术,测定桑螵蛸脂质提取物的化学成分及其相对比例。采用纸片扩散法和琼脂平板稀释法观察桑螵蛸脂类提取物对铜绿假单胞菌的抑菌效应并测定最小抑制浓度(MIC)。采用结晶紫染色法和扫描电镜(SEM)进行抑制生物被膜的试验。结论:桑螵蛸脂类提取物中含有16种化合物,最丰富的成分分别是倍半萜类化合物、单萜和微量芳香族化合物。桑螵蛸脂类提取物对铜绿假单胞菌的MIC为4 mg/ml,对铜绿假单胞菌生物被膜的抑制作用明显。 | Wen-dong WANG Nan-nan ZHANG Warren CHANDA Min LIU Syed Riaz ud DIN Yun-peng DIAO Lei LIU Jing CAO Xiao-li WANG Xing-yun LI An-hong NING Min HUANG Min-tao ZHONG | 2018 | Journal of Zhejiang University-Science B(Biomedicine & Biotechnology)2018，19，5： | 6 |
| 3 | Construction of the recombinant adenovirus vector carrying antisense multidrug resistance gene显示文摘BACKGROUND: Multidrug resistance proteins serve as transporters for chemical drugs in human malignancies. The objective of this study was to construct a homologous recombinant adenovirus carrying a reversal fragment of multidrug resistance gene 1 (mdr1) gene cDNA sequence. METHODS: The fragment of the mdr1 gene from the plasmid pHaMDRI-1 carrying the whole human mdr1 cDNA sequence was inserted reversely into the shuttle plasmid pAdTrack-CMV of adenoviral vector system AdEasy. The homologous recombination process was taken place in E. coli BJ5183 with the backbone plasmid pAdEasy-1. After packaging in 293 cells, recombinant adenoviral plasmid was generated. The recombinant adenoviral plasmid was identified by polymerase chain reaction (PCR), restriction endonucleases digest, DNA sequence analysis and fluorescence microscopic photograph, respectively. RESULTS: The recombinant adenovirus pAdEasy-GFPASmdr1 was successfully constructed and identified by PCR, restriction digest, and sequencing with strong green fluorescence expression in fluorescence microscopic photograph. CONCLUSIONS: The recombinant adenoviral mdr1 vector would introduce the antisense mdr1 gene into the human multidrug resistance hepatocellular cell fine effectively, which would provide an experimental basis to study the multidrug resistance in human hepatocellular carcinoma. | Bo Li, Xing-Hua Gou, Lin Chen, De-Hua Li, Yong-Heng Zhao, Lei Han, Lan-Ying Zhao and Jian-ping Gong Department of Hepatobiliary Surgery, Second Affiliated Hospital, Chongqing University of Medical Sciences, Chongqing 400010, China Genetic Engineering Laboratory, Chengdu Diao Group Co. Ltd., Chengdu 610041, China and Department of General Surgery, West China Hospital, Sichuan University, Chengdu 610041, China | 2006 | Hepatobiliary & Pancreatic Diseases International2006，5，1： | 6 |
| 4 | Involvement of cytochrome P450 3A4 and P-glycoprotein in first-pass intestinal extraction of omeprazole in rabbits显示文摘 | Hai-ming FANG Jian-ming XU Qiao MEI Lei DIAO Mo-li CHEN Juan JIN Xin-hua XU | 2009 | Acta Pharmacologica Sinica2009，30，11： | 4 |
| 5 | Culturable plant pathogenic fungi associated with sugarcane in southern China显示文摘Sugarcane(Saccharum officinarum)is one of the oldest crops cultivated by mankind.Numerous fungal taxa have been reported from this host,although most were not identified beyond genus level.In this study,we explored 31 sampling sites in Guangxi and Guangdong Provinces(China),and collected 370 diseased samples from leaves and roots of sugarcane,from which 762 strains were isolated.Our preliminary analysis based on internal transcribed spacer(ITS)sequences suggested that these isolates belonged to 143 species in 51 genera,but we could not assign 129 strains.Bipolaris,Chaetomium,Curvularia,Phoma and Nigrospora represented the top five most common genera identified,while 27 rare genera comprised only one identified species.In this study,we chose above mentioned common genera for in-depth morphological observations and multi-locus analyses,in order to identify these strains to species level.In this paper,we described one new genus,32 new species,and reported 19 new records for China and the asexual morph of Chaetomium olivaceum.Hitherto,this is the most comprehensive study with molecular identification and illustration of fungi associated with sugarcane,which greatly improves our understanding of culturable mycota associated with this host. | Mubashar Raza Zhi-Feng Zhang Kevin D.Hyde Yong-Zhao Diao Lei Cai | 2019 | Fungal Diversity2019，，6： | 4 |
| 6 | A protective effect of melatonin on intestinal permeability is induced by diclofenac via regulation of mitochondrial function in mice显示文摘 | Qiao MEI Lei DIAO Jian-ming XU Xiao-chang LIU Juan JIN | 2011 | Acta Pharmacologica Sinica2011，32，4： | 3 |
| 7 | Numerical Analysis on Nanoparticles-laden Gas Film Thrust Bearing显示文摘Nanoparticles can be taken as additives and added into various fluids to improve their lubricating performances. At present, researches in this area are mainly concentrated on the improvement effects of nanoparticles on the lubricating performances of liquid such as oil and water. Nanoparticles will also affect gas lubrication, but few related studies have been reported. Nanoparticles-laden gas film (NLGF) is formed when adding nanoparticles into gas bearing. Then, the lubricating performances of gas bearing including pressure distribution and load-carrying capacity will change. The variations of pressure distribution and load-carrying capacity in nanoparticles-laden gas film thrust bearing are investigated by numerical method. Taking account of the compressibility of gas and the interactions between gas and nanoparticles, a computational fluid dynamics model based on Navier-Stokes equations is applied to simulate the NLGF flow. The effects of inlet nanoparticles volume fraction and orifice radius on film pressure distribution and load-carrying capacity of the NLGF are calculated. The numerical calculation results show that both of the film land pressure and the maximum film pressure both increase when the nanoparticles are added into gas bearing, and the film pressures increase with the rising of the inlet nanoparticles volume fraction. The nanoparticles have an enhancement effect on load-carrying capacity of the studied bearing, and the enhancement effect becomes greater as the film thickness decrease. Therefore, nanoparticles can effectively improve the lubricating performance of gas bearing. The proposed research provides a theoretical basis for the design of new-type nanoparticles-laden gas film bearings. | YANG Zhiru DIAO Dongfeng YANG Lei | 2013 | Chinese Journal of Mechanical Engineering2013，26，4： | 3 |
| 8 | Chronic exposure to ethanol in male mice may be associated with hearing loss in offspring显示文摘 | Fei Liang Lei Diao Nan Jiang Jin Zhang Hui-Jun Wang Wen-Hao Zhou Guo-Ying Huang Duan Ma | 2015 | Asian Journal of Andrology2015，17，6： | 3 |
| 9 | Association of assisted reproductive technology, germline de novo mutations and congenital heart defects in a prospective birth cohort study显示文摘Emerging evidence suggests that children conceived through assisted reproductive technology(ART)have a higher risk of congenital heart defects(CHDs)even when there is no family history.De novo mutation(DNM)is a well-known cause of sporadic congenital diseases;however,whether ART procedures increase the number of germline DNM(gDNM)has not yet been well studied.Here,we performed whole-genome sequencing of 1137 individuals from 160 families conceived through ART and 205 families conceived spontaneously.Children conceived via ART carried 4.59 more gDNMs than children conceived spontaneously,including 332 paternal and 1.26 maternal DNMs,after correcting for parental age at conception,cigarette smoking,alcohol drinking,and exercise behaviors.Paternal DNMs in offspring conceived via ART are characterized by C>T substitutions at CpG sites,which potentially affect protein-coding genes and are significantly associated with the increased risk of CHD.In addition,the accumulation of non-coding functional mutations was independently associated with CHD and 87.9% of the mutations were originated from the father.Among ART offspring,infertility of the father was associated with elevated paternal DNMs;usage of both recombinant and urinary follicle-stimulating hormone and high-dosage human chorionic gonadotropin trigger was associated with an increase of maternal DNMs.In sum,the increased gDNMs in offspring conceived by ART were primarily originated from fathers,indicating that ART itself may not be a major reason for the accumulation of gDNMs.Our findings emphasize the importance of evaluating the germline status of the fathers in families with the use of ART. | Cheng Wang Hong Lv Xiufeng Ling Hong Li Feiyang Diao Juncheng Dai Jiangbo Du Ting Chen Qi Xi Yang Zhao Kun Zhou Bo Xu Xiumei Han Xiaoyu Liu Meijuan Peng Congcong Chen Shiyao Tao Lei Huang Cong Liu Mingyang Wen Yangqian Jiang Tao Jiang Chuncheng Lu Wei Wu Di Wu Minjian Chen Yuan Lin Xuejiang Guo Ran Huo Jiayin Liu Hongxia Ma Guangfu Jin Yankai Xia Jiahao Sha Hongbing Shen Zhibin Hu | 2021 | Cell Research2021，31，8： | 2 |
| 10 | A potent PGK1 antagonist reveals PGK1 regulates the production of IL-1βand IL-6显示文摘Glycolytic metabolism enzymes have been implicated in the immunometabolism field through changes in metabolic status. PGK1 is a catalytic enzyme in the glycolytic pathway. Here, we set up a high-throughput screen platform to identify PGK1 inhibitors. DC-PGKI is an ATPcompetitive inhibitor of PGK1 with an affinity of Kd= 99.08 nmol/L. DC-PGKI stabilizes PGK1in vitro and in vivo, and suppresses both glycolytic activity and the kinase function of PGK1. In addition,DC-PGKI unveils that PGK1 regulates production of IL-1β and IL-6 in LPS-stimulated macrophages.Mechanistically, inhibition of PGK1 with DC-PGKI results in NRF2(nuclear factor-erythroid factor 2-related factor 2, NFE2L2) accumulation, then NRF2 translocates to the nucleus and binds to the proximity region of Il-1β and Il-6 genes, and inhibits LPS-induced expression of these genes. DC-PGKI ameliorates colitis in the dextran sulfate sodium(DSS)-induced colitis mouse model. These data support PGK1 as a regulator of macrophages and suggest potential utility of PGK1 inhibitors in the treatment of inflammatory bowel disease. | Liping Liao Wenzhen Dang Tingting Lin Jinghua Yu Tonghai Liu Wen Li Senhao Xiao Lei Feng Jing Huang Rong Fu Jiacheng Li Liping Liu Mingchen Wang Hongru Tao Hualiang Jiang Kaixian Chen Xingxing Diao Bing Zhou Xiaoyan Shen Cheng Luo | 2022 | Acta Pharmaceutica Sinica B2022，12，11： | 2 |
| 11 | Bletilla striata Polysaccharide Stimulates Inducible Nitric Oxide Synthase and Proinflammatory Cytokine Expression in Macrophages显示文摘 | Huajia Diao Xin Li Jiangning Chen Yi Luo Xi Chen Lei Dong Chunming Wang Chenyu Zhang Junfeng Zhang | 2007 | Journal of Bioscience and Bioengineering2007，，2： | 1 |
| 12 | Dynamics of the SARS-CoV-2 antibody response up to 10 months after infection显示文摘COVID-19 caused by SARS-CoV-2 infection has caused substantial morbidity and mortality worldwide and paralyzed the international economy.Understanding the magnitude and duration of the antibody response to SARS-CoV-2 is important to achieve a balance between curbing the pandemic and minimizing adverse effects on society.1 Although the antibody response to SARS-CoV-2 within 9 months has been extensively studied,2,3,4,5,6 little is known about the magnitude and kinetics of antibody responses for over 9 months.Moreover,with limited observations over 9 months(n<100),2,7,8 several studies have produced inconsistent conclusions about antibody dynamics,suggesting different rates of antiviral antibody positivity at the last follow-up.2,7,8 These studies have been limited by a lack of measurement of neutralizing antibodies(NAbs),7 of inclusion of mild or asymptomatic cases,2,8 and of further exploration of potential predisposing factors for antibody dynamics.2,7 Considering the individual heterogeneity(such as disease severity)8 and time-dependent nature1 of the immune response,in-depth characterization of SARS-CoV-2 antibody kinetics across disease severity groups over a long period is urgently needed.Therefore,we repeatedly tested IgM,IgG,viral spike protein receptor-binding dom(anti-RBD)IgG,and NAb titers in COVID-19 patients during a follow-up period of up to 10 months and explored potential predisposing factors of antibody titers during follow-up. | Hao Wang Yu Yuan Mingzhong Xiao Li Chen Youyun Zhao Haiwei Zhang Pinpin Long Yana Zhou Xi Xu Yanshou Lei Bihao Wu Tingyue Diao Hao Cai Li Liu Zuoyu Shao Jingzhi Wang Yansen Bai Kai Wang Miao Peng Linlin Liu Shi Han Fanghua Mei Kun Cai Yake Lei An Pan Chaolong Wang Rui Gong Xiaodong Li Tangchun Wu | 2021 | Cellular & Molecular Immunology2021，18，7： | 1 |
| 13 | A Polysaccharide Isolated from the Medicinal Herb Bletilla striata Induces Endothelial Cells Proliferation and Vascular Endothelial Growth Factor Expression in vitro显示文摘 | Chunming Wang Jiantao Sun Yi Luo Weihua Xue Huajia Diao Lei Dong Jiangning Chen Junfeng Zhang | 2006 | Biotechnology Letters2006，，8： | 1 |
| 14 | Effects of Pb and Mg doping in Al 2 O 3 -supported Pd catalyst on direct oxidative esterification of aldehydes with alcohols to esters显示文摘 | Yanyan Diao Ruiyi Yan Suojiang Zhang Pu Yang Zengxi Li Lei Wang Haifeng Dong | 2009 | Journal of Molecular Catalysis A Chemical2009，，1： | 1 |
| 15 | Network system research and design for 100% low floor light rail vehicle显示文摘 | Mei Ying Lijun Diao Wang Lei | 2008 | 2008，，： | 1 |
| 16 | LncRNA MIAT impairs cardiac contractile function by acting on mitochondrial translocator protein TSPO in a mouse model of myocardial infarction显示文摘Dear Editor,Long non-coding RNA MIAT(IncR-MIAT)has recently been identified as a risk factor for myocardial infarction(Ml).1 However,how IncR-MIAT controls Ml remained yet to be determined.To shed light on this issue,we firstly detected the expression of IncR-MIAT using qRT-PCR and found a robust elevation(>5-fold)of IncR-MIAT level in heart of Ml mice relative to sham-operated control counterparts(Supplementary Fig.S1a). MIAT in NMVCs(Supplementary Fig.Sic). | Xue Bai Chao Yang Lei Jiao Hongtao Diao Ziyu Meng Lei Wang Hao Cui Lihua Sun Yong Zhang Baofeng Yang | 2021 | Signal Transduction and Targeted Therapy2021，6，6： | 1 |
| 17 | The adaptor pro- tein MITA links virus-sensing receptors to IRF3 tran scription factor activation显示文摘 | Zhong B Yang Y Li S Wang Y Y Li Y Diao F Lei C He X Zhang L Tien P Shu H B | 2008 | Immunity2008，29，： | 1 |
| 18 | Pulsed LD side-pumped MgO:LN electro-optic cavity-dumped 1123 nm Nd: YAG laser with short pulse width and high peak power显示文摘We report a cavity-dumped 1123 nm laser with narrow pulse width and high peak power by an Mg O: LN crystal electrooptic(EO) modulator. Based on the structural optimization design of a folded biconcave cavity using the 808 nm pulsed laser diode(LD) side-pumped ceramic Nd: YAG rod, output pulses with maximum pulse energy and peak power up to39.6 m J and 9.73 MW were obtained, corresponding to 100 Hz repetition rate and 4.07 ns pulse width. The instabilities of pulse width and pulse energy were ±1.55% and ±2.06%, respectively. At the highest repetition rate of 1 kHz, the pulse energy, pulse width, and peak power were 11.3 mJ, 5.05 ns, and 2.24 MW, respectively. The instabilities of pulse width and pulse energy were ±2.65% and ±3.47%, respectively. | Yang Bai Bing Bai Diao Li Yanxiao Sun Jianlin Li Lei Hou Mingxuan Hu Jintao Bai | 2018 | High Power Laser Science and Engineering2018，6，1： | 1 |
| 19 | Aberrant Notch signalling contributes to hypertrophic scar formation by modulating the phenotype of keratinocytes显示文摘 | Bing Li Chao Gao Jian‐Sheng Diao Da‐Lei Wang Fei‐Fei Chu Yang Li Gang Wang Shu‐Zhong Guo Wei Xia | 2016 | Exp Dermatol2016，，2： | 1 |
| 20 | A nomogram predicting clinical pregnancy in the first fresh embryo transfer for women undergoing in vitro fertilization and intracytoplasmic sperm injection(IVF/ICSI) treatments显示文摘The extent to which factors affect the probability of clinical pregnancy in the first fresh embryo transfer after assisted conception is unknown.In order to examine the predictors of clinical pregnancy,a retrospective cohort study was launched between January 1,2013 and December 31,2016 in four infertility clinics including 19837 in vitro fertilization and intracytoplasmic sperm injection(IVF/ICSI)fresh cycles with known outcomes and relevant records.A multivariable logistic regression was used to select the most significant predictors in the final nomogram for predicting clinical pregnancy.Furthermore,the model was validated by an independent validation set and the performance of the model was evaluated by the receiver operating characteristic(ROC)curves along with the area under the ROC curve(AUC)and calibration plots.In a training set including 17854 participants,we identified that female age,tubal factor,number of embryos transferred,endometrial thickness and number of good-quality embryos were independent predictors for clinical pregnancy.We developed a nomogram using these five factors and the predictive ability was 0.66 for AUC(95%CI=0.64−0.68),which was independently validated in the validation set(AUC=0.66,95%CI=0.65−0.68).Our results show that some specific factors can be used to provide infertile couples with an accurate assessment of clinical pregnancy following assisted conception and facilitate to guide couples and clinicians. | Fang Wu Feng Liu Yichun Guan Jiangbo Du Jichun Tan Hong Lv Qun Lu Shiyao Tao Lei Huang Kun Zhou Yankai Xia Xinru Wang Hongbing Shen Xiufeng Ling Feiyang Diao Zhibin Hu Guangfu Jin | 2019 | The Journal of Biomedical Research2019，33，6： | 1 |
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