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| 1 | Effect of constant compressive stress induced by imitating tuina stimulation with various durations on the cell cycle,cellular secretion,apoptosis,and expression of myogenic differentiation and myogenic factor 5 of rat skeletal muscle cells in vitro显示文摘OBJECTIVE:To investigate the effect of constant compressive stress induced by imitating Tuina stimulation with various durations on the cell cycle,cellular secretion,apoptosis,and expression of myogenic regulatory factors(MRFs),myogenic factor 5(Myf5)and myogenic differentiation(MyoD)of rat skeletal muscle cells(RSkMCs)in vitro.METHODS:Third passage RSkMCs were subjected to constant compressive stresses with various durations at 2000μstrain for 15,30,60,90,and 120 min via a four-point bending system.The control group(CG)was cultured in the absence of mechanical loading.Alterations of the cell cycle and apoptosis rate were detected by flow cytometry(FCM).The concentrations of interleukin 6(IL-6)/prostaglandin E2(PGE2)and nitric oxide(NO)in supernatants were determined by enzyme-linked immunosorbent assays and the nitrate reductase method,respectively.Expression of Myf5 and MyoD was detected by immunohistochemistry.RESULTS:Compared with the CG,a significant alteration was observed in the synthesis phase fraction(SPF)(P<0.01).The SPF and proliferation index(PI)were reduced from 15 to 90 min,but reached levels similar to those at 120 min.Apoptosis was increased significantly at 30 min(P<0.05)and especially at 90 and 120 min(P<0.01).Expression of MyoD and Myf5 was increased significantly at 15,30,and 90 min(P<0.01).Compared with 15 and 30 min,MyoD and Myf5 expression at 60 and 120 min was decreased significantly(P<0.01).Compared with 60 min,M yoD expression at 90 min was increased significantly(P<0.05),whereas MyoD and Myf5 expression at 120 min was significantly lower(P<0.05).The IL-6 concentration was increased at 60 min compared with the CG and 15 min(P<0.05),whereas the concentrations of PGE2 and NO were the highest at 15 and 30 min,respectively,compared with the CG and other time points(P<0.05).CONCLUSION:The cell cycle,secretion,apoptosis,and Myf5 and MyoD expression of RSkMCs were regulated by compressive stress in a time-dependent manner.SPF and PI were inhibited at short durations(<90 min),but NO and PGE2 secretion was the highest at shorter durations(<30 min).With the prolongation of stimulation time,SPF,PI,and apoptosis were increased,but Myf5 and MyoD expression was decreased gradually at 15-30 min. | Lu Qunwen Su Chengguo Liu Huahui Luo Caigui Yan Bohua | 2020 | Journal of Traditional Chinese Medicine2020，40，4： | 1 |
| 2 | Spatial Layout of Modern Agriculture in Tianjin Binhai New Area显示文摘As the trial site of comprehensive reform of China, Tianjin Binhai New Area (TBNA) is facing the problem of how to promote the balanced urban and | YE Wei,Chief Planner,Branch of Tianjing Binhai New Area,Tianjin Institute of Urban Planning and Design,Tianjin,P. R. China. ZHU Liming,Deputy Director and Senior Planner,Branch of Tianjin Binhai New Area,Tianjing Institute of Urban Planning and Design,Tianjin,P. R. China. LIU Shaoyin,Director of the Strategic Planning Program,Tianjin Huahui Consulting Ltd. of Environmental Planning and Design,Tianjin,P. R. China. Editorial Department | 2007 | China City Planning Review2007，16，4： | 0 |
| 3 | Manufacturable Novel Nanogrease with Superb Physical Properties显示文摘High-performance nanogrease manufactured from carbon nanomaterials is observed to be stable and homogeneous and have superb physical properties,such as thermal and electrical conductivities,compared with current commercial greases made of lithium,calcium,and aluminum.For the first time,carbon nanomaterials have been observed to disperse well as the sole thickeners in oil systems,e.g.,polyalphaolefin and polyester(ROYCO),without the aid of any chemical surfactants.Three-dimensional percolation network structures that exist among carbon nanomaterials are easily scalable,which can be attributed to the intermolecular van der Waals forces.Moreover,the introduction of hydrogen bonding in any form to grease significantly increases its thermal and electrical conductivities and substantially reduces the weight percentage of carbon nanomaterials needed to fabricate stable grease.For example,loading of only 1.4 wt%hydroxyl-functionalized multiwalled carbon nanotube(MWNT-OH)with Krytox XHT750 oil leads to a 37.8%increase in thermal conductivity.Moreover,75%glycerol,25% water,and 4.5 wt% MWNT-OH yielded the lowest electrical resistivity of 10.0 Ω cm.This finding can be extended to hydrogen bonding materials with functional groups,such as OH,COOH,F,and NH.The nanogrease reported in this study has been manufactured using the three-roll mill method,which is an easy and cost-effective method,as the loading weight percentage of carbon nanomaterials to fabricate stable grease decreases from 12 wt% to 3-4 wt%.Furthermore,the process is easily scalable,reproduced,and optimized.This novel high-performance nanogrease has a high commercial value and numerous applications and could replace current commercial greases. | Hammad Younes Ding Lou Haiping Hong Huahui Chen Hongtao Liu Yinhuai Qiang | 2021 | Nanomanufacturing and Metrology2021，4，4： | 0 |
| 4 | The Linear Regression Method for Improving Magnetic Parameter of the Electroless CoFeB Films显示文摘免职率的动力学方程被实现帮助解释一些为在聚酯塑料上在 CoFeB 电影的免职期间观察的结构负责的机制。无电的 CoFeB 电影的 plating 率是钠 tetrahydroborate, plating 洗澡的酸碱值, plating 温度和金属性的比率的集中的功能。估计的回归系数,信心间隔,剩余错误和信心间隔被计算机节目证实。plating 洗澡的最佳的作文被获得,电影的动态电磁的参数在 2 10 GHz 范围被测量。在 2 GHz,渗透,因为还原剂的集中是 1 g · L −1 ,无电的 CoFeB 电影的磁性的损失分别地是 304,76.6。 | LIU Changhui HE Huahui SHEN Xiang LI Haihua | 2008 | Journal of Wuhan University of Technology(Materials Science)2008，23，2： | 0 |
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