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| 1 | Establishment and Characterization of a Cell Based Artificial Antigen-Presenting Cell for Expansion and Activation of CD8^+ T Cells Ex Vivo显示文摘Artificial antigen-presenting cells are expected to stimulate the expansion and acquisition of optimal therapeutic features of T cells before infusion. Here CD32 that binds to a crystallizable fragment of IgG monoclonal antibody was genetically expressed on human K562 leukemia cells to provide a ligand for T-cell receptor. CD86 and 4-1BBL,which are ligands of co-stimulating receptors of CD28 and 4-1BB,respectively,were also expressed on K562 cells. Then we accomplished the artificial antigen-presenting cells by coupling K32/CD86/4-1BBL cell with OKT3 monoclonal antibody against CD3,named K32/CD86/4-1BBL/OKT3 cells. These artificial modified cells had the abilities of inducing CD8+ T cell activation,promoting CD8+ T cell proliferation,division,and long-term growth,inhibiting CD8+ T cell apoptosis,and enhancing CD8+ T cell secretion of IFN-γ and perforin. Furthermore,antigen-specific cytotoxic T lymphocytes could be retained in the culture stimulated with K32/CD86/4-1BBL/OKT3 cells at least within 28 days. This approach was robust,simple,reproducible and economical for expansion and activation of CD8+ T cells and may have important therapeutic implications for adoptive immunotherapy. | Weijuan Gong Mingchun Ji Zhengfeng Cao Liheng Wang Yayun Qian Maozhi Hu Li Qian Xingyuan Pan | 2008 | Cellular & Molecular Immunology2008，5，1： | 5 |
| 2 | Immobilized MHC class I chain-related protein A synergizes with IL-15 and soluble 4-1BB ligand to expand NK cells with high cytotoxicity ex vivo显示文摘Major histocompatibility complex(MHC)class I chain-related protein A(MICA),which is a ligand for human NKG2D,is expressed by a variety of epithelial tumor cells and promotes the activation of natural killer(NK),CD81 and cd-T cells.Although ectopic expression of MICA on tumor cells elicits anti-tumor responses,soluble MICA downregulates the activities of lymphocytes.In this study,we showed that recombinant,immobilized MICA(iMICA)molecules coated on plastic wells weakly promote peripheral NK cell activation,secretion of interferon(IFN)-c and degranulation without inducing apoptosis.In addition,iMICA synergized with IL-15 and soluble 4-1BB ligand(s4-1BBL)to expand NK cells 25-to 42-fold in a 13-day culture,whereas NK cells stimulated only with IL-15 and s4-1BBL expanded 10-to 16-fold.In contrast toNKcells expanded by IL-15 and s4-1BBL stimulation,NKcells expanded long term in the presence of iMICA exhibited increased cytotoxicity against leukemia cells.These results suggest that large numbers ofNKcells with high cytotoxicity can be generated by stimulation with IL-15 and s4-1BBL in the presence of iMICA and that these cells can be used for adoptive cancer immunotherapy. | Weijuan Gong Weiming Xiao Li Qian Chunxiang Gong Maozhi Hu Xianyuan Pan Mingchun Ji | 2010 | Cellular & Molecular Immunology2010，7，6： | 2 |
| 3 | Avian reovirus triggers autophagy in primary chicken fibroblast cells and Vero cells to promote virus production显示文摘 | Songshu Meng Ke Jiang Xiaorong Zhang Miao Zhang Zhizhi Zhou Maozhi Hu Rui Yang Chenli Sun Yantao Wu | 2012 | Archives of Virology2012，，4： | 1 |
| 4 | Cycling Attacks against Homomorphic Cryptosystems显示文摘We study security of some homomorphic cryptosystems with similar algebraic structure. It is found out that those cryptosystems have special common properties. Based on these properties, we pose two cycling attacks and point out some parameters under which the attacks are efficient. It is verified that randomly selected parameters almost impossibly submit to such attacks. Anyhow, two effective methods are given to construct weak parameters for certain homomorphic cryptosystems, and two moduli over 1 024 bits computed by them are shown to be vulnerable to our cycling attacks. It is concluded that strong primes should be used to avert weak parameters. | WANG Lin XU Maozhi HU Zhi YUE Zhihong | 2008 | Wuhan University Journal of Natural Sciences2008，13，6： | 0 |
| 5 | 以边缘功能化的氮化碳纳米片为非金属光催化剂实现高效饮用水获得显示文摘文章简介清洁水资源短缺是全世界面临的棘手问题,科学家通过多种途径试图寻求一种高效、节能的光催化材料,以实现有效杀菌净水。非金属光催化剂,具有成本低,酸碱耐受力高等众多优势,尤其是可以有效避免金属向水中溶出引发的二次污染,是一类非常有潜力的新型催化剂。但是目前的非金属光催化剂效率仍然不高,无法与金属基的催化剂相抗衡。 | 滕镇远 杨乃亮 Hongying Lv Sicong Wang Maozhi Hu 王谧胤 王丹 汪国秀 | 2020 | 科学新闻2020，，2： | 0 |
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