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| 1 | Environmental surveillance of SARS-CoV-2 RNA in wastewater systems and related environments in Wuhan:April to May of 2020显示文摘Wastewater-based epidemiology(WBE)has emerged as an effective environmental surveillance tool in monitoring fecal-oral pathogen infections within a community.Congruently,SARS-Co V-2,the etiologic agent of COVID-19,has been demonstrated to infect the gastrointestinal tissues,and be shed in feces.In the present study,SARS-Co V-2 RNA was concentrated from wastewater,sludge,surface water,ground water,sediment,and soil samples of municipal and hospital wastewater systems and related environments in Wuhan during the COVID-19 middle and low risk periods,and the viral RNA copies quantified using reverse transcription quantitative polymerase chain reaction(RT-q PCR).From the findings of this study,during the middle risk period,one influent sample and three secondary effluents collected from waste water treatment plant 2,as well as two samples from Jinyintan Hospital wastewater system influent were SARS-Co V-2 RNA positive.One sludge sample collected from Guanggu Branch of Tongji Hospital,which was obtained during the low risk period,was also positive for SARS-Co V-2 RNA.These study findings demonstrate the significance of WBE in continuous surveillance of SARS-Co V-2 at the community level,even when the COVID-19 prevalence is low.Overall,this study can be used as an important reference for contingency management of wastewater treatment plants and COVID-19 prevention and control departments of Wuhan. | Lu Zhao Evans Atoni Raphael Nyaruaba Yao Du Huaiyu Zhang Oscar Donde Doudou Huang Shuqi Xiao Nanjie Ren Teng Ma Zhu Shu Zhiming Yuan Lei Tong Han Xia | 2022 | Journal of Environmental Sciences2022，34，2： | 1 |
| 2 | Integrated site-specific quantification of faecal bacteria and detection of DNA markers in faecal contamination source tracking as a microbial risk tracking tool in urban Lake ecosystems显示文摘The presence of feacal-derived pathogens in water is responsible for several infectious diseases and deaths worldwide. As a solution, sources of fecal pollution in waters must be accurately assessed, properly determined and strictly controlled. However, the exercise has remained challenging due to the existing overlapping characteristics by different members of faecal coliform bacteria and the inadequacy of information pertaining to the contribution of seasonality and weather condition on tracking the possible sources of pollution. There are continued ef forts to improve the Faecal Contamination Source Tracking(FCST) techniques such as Microbial Source Tracking(MST). This study aimed to make contribution to MST by evaluating the efficacy of combining site specific quantification of faecal contamination indicator bacteria and detection of DNA markers while accounting for seasonality and weather conditions' eff ects in tracking the major sources of faecal contamination in a freshwater system(Donghu Lake, China). The results showed that the use of cyd gene in addition to lacZ and uidA genes differentiates E. coli from other closely related faecal bacteria. The use of selective media increases the pollution source tracking accuracy. BSA addition boosts PCR detection and increases FCST efficiency. Seasonality and weather variability also influence the detection limit for DNA markers. | Oscar Omondi DONDE 田翠翠 肖邦定 | 2018 | Journal of Oceanology and Limnology2018，36，5： | 0 |
| 3 | Improved lakeshore sediment microenvironment and enhanced denitrification efficiency by natural solid carbon sources显示文摘At present,the high level of nitrate in tailwater of wastewater treatment plants(WWTPs)has continued to cause serious environmental problems.In current study,wood chips and bark,serving as natural solid carbon source(NSCS),were applied to enhance the denitrification capacity of lakeshore sediment to remove superabundant nitrate in tailwater.The results show that NSCS had a positive effect on denitrification process for lakeshore sediment and increased the total phosphorus removal capacity by 212%-601%.Comparatively,wood chips were better in enhancing denitrification ability of lakeshore sediment for a longer time.At the 70th day,the nitrate elimination(NEL)of the wood chip-sediment integrated system(WSIS)was 20%and 9%higher than the control system(CS)and bark-sediment integrated system(BSIS),respectively.For the WSIS,the microbial activity and the abundance of nicotinamide adenine dinucleotide(NADH)were significantly increased,and the sediment anaerobic zone was rapidly formed,which were conducive to the denitrification of the sediment.At the DNA level,both groups presented an inconsistency in the abundance of functional denitrification genes with the NEL.At the enzyme level,wood chips significantly increased the nitrate and nitrite reductase activities of the sediment,and promoted denitrification.From the perspective of microbial communities,wood chips highly promoted the abundance of norank_f_BIrii41 Proteobacteria and Ruminclostridum more than other denitrifying and potential denitrifying bacteria in the process of sediment denitrification enhancement. | Shihao Gong Oscar Omondi Donde Qijia Cai Xingqiang Wu Kezheng Song Chunbo Wang Pei Hong Bangding Xiao Cuicui Tian | 2022 | International Journal of Sediment Research2022，37，6： | 0 |
| 4 | Effects of organic carbon consumption on denitrifier community composition and diversity along dissolved oxygen vertical profiles in lake sediment surface显示文摘At present,the understanding of the dynamics of denitrifiers at different dissolved oxygen(DO)layers under organic carbon consumption within the surface sediments remains inadequate.In this study,high-throughput sequencing and quantitative PCR targeting nirS gene were used to analyze the denitrifier abundance dynamics,community composition,and structure for aerobic(DO 0.5-6.9 mg/L),hypoxic-anoxic(DO 0-0.5 mg/L),and anoxic(DO 0 mg/L)layers in surface sediments under organic carbon consumption.Based on the analysis of nirS gene abundance,the values of denitrifying bacteria decreased with organic carbon consumption at different DO layers.When the bacterial species abundance at the genus level were compared between the high-carbon and low-carbon sediments,there was significant increase in 6 out of 36,7 out of 36 and 6 out of 36 genera respectively for the aerobic,hypoxic-anoxic and anoxic layers.On the other hand,14 out of 36,9 out of 36 and 15 out of 36 genera showed significant decrease in bacterial species abundance respectively for the aerobic,hypoxic-anoxic and anoxic layers.Additionally,14 out of 36,20 out of 36,and 15 out of 36 genera had no change in bacterial species abundance respectively for the aerobic,hypoxic-anoxic,and anoxic layers.This indicates that the carbon utilization ability of different denitrifiers on each DO layers was generally different from each other.Diversity of denitrifying bacteria also presented significant differences in different DO layers between the high-and low-carbon content sediment layers.Moreover,under the high-carbon and low-carbon content,the abundance of nirS gene showed a high peak within the hypoxic-anoxic regions,suggesting that this region might be the main distribution area for the denitrifying bacteria within the surface sediments.Furthermore,community of unique denitrifiers occurred in different DO layers and the adaptive changes of the denitrifier community followed the organic carbon consumption. | HONG Pei GONG Shihao WANG Chunbo SHU Yilin WU Xingqiang TIAN Cuicui Oscar Omondi DONDE CAI Pei WU Huaming XIAO Bangding | 2020 | Journal of Oceanology and Limnology2020，38，3： | 0 |
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