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| 1 | SARS-CoV-2 Does Not Replicate in Aedes Mosquito Cells nor Present in Field-Caught Mosquitoes from Wuhan显示文摘Dear Editor,Severe acute respiratory syndrome coronavirus 2(SARSCoV-2), the etiologic agent of COVID-19, is an enveloped,positive-sense single-stranded RNA virus that first discovered in December 2019 from a seafood market in Wuhan, China(Zhou et al. 2020). | Han Xia Evans Atoni Lu Zhao Nanjie Ren Doudou Huang Rongjuan Pei Zhen Chen Jin Xiong Raphael Nyaruaba Shuqi Xiao Bo Zhang Zhiming Yuan | 2020 | Virologica Sinica2020，35，3： | 1 |
| 2 | Development of a new field-deployable RT-qPCR workflow for COVID-19 detection显示文摘Background:Outbreaks of coronavirus disease 2019(COVID-19)have been recorded in different countries across the globe.The virus is highly contagious,hence early detection,isolation,and quarantine of infected patients will play an important role in containing the viral spread.Diagnosis in a mobile lab can aid to find infected patients in time.Methods:Here,we develop a field-deployable diagnostic workflow that can reliably detect COVID-19.Instruments used in this workflow can easily fit in a mobile cabin hospital and also be installed in the community.Different steps from sample inactivation to detection were optimized to find the fastest steps and portable instruments in the detection of COVID-19.Each step was compared to that of the normal laboratory diagnosis setup.Results:From the results,our proposed workflow(80 min)was two times faster compared to that of the normal laboratory workflow(183 min)and a maximum of 32 samples could be detected at each run.Additionally,we showed that using 1%Rewocid WK-30 could inactivate the novel coronavirus directly without affecting the overall detection results.Comparison of our workflow using an in-house assay to that of a commercially acquired assay produced highly reliable results.From the 250 hospital samples tested,there was a high concordance 247/250(98.8%)between the two assays.The in-house assay sensitivity and specificity were 116/116(100%)and 131/134(97.8%)compared to that of the commercial assay.Conclusion:Based on these results,we believe that our workflow is fast,reliable,adaptable and most importantly,field-deployable. | Raphael Nyaruaba Bo Zhang Caroline Muema Elishiba Muturi Greater KOyejobi Jin Xiong Bei Li Zheng-Li Shi Caroline Mwaliko Jun-Ping Yu Xiao-Hong Li Ya-Nan Zhang Hong-Ping Wei | 2021 | Life Research2021，4，3： | 1 |
| 3 | Environmental surveillance of SARS-CoV-2 RNA in wastewater systems and related environments in Wuhan:April to May of 2020显示文摘Wastewater-based epidemiology(WBE)has emerged as an effective environmental surveillance tool in monitoring fecal-oral pathogen infections within a community.Congruently,SARS-Co V-2,the etiologic agent of COVID-19,has been demonstrated to infect the gastrointestinal tissues,and be shed in feces.In the present study,SARS-Co V-2 RNA was concentrated from wastewater,sludge,surface water,ground water,sediment,and soil samples of municipal and hospital wastewater systems and related environments in Wuhan during the COVID-19 middle and low risk periods,and the viral RNA copies quantified using reverse transcription quantitative polymerase chain reaction(RT-q PCR).From the findings of this study,during the middle risk period,one influent sample and three secondary effluents collected from waste water treatment plant 2,as well as two samples from Jinyintan Hospital wastewater system influent were SARS-Co V-2 RNA positive.One sludge sample collected from Guanggu Branch of Tongji Hospital,which was obtained during the low risk period,was also positive for SARS-Co V-2 RNA.These study findings demonstrate the significance of WBE in continuous surveillance of SARS-Co V-2 at the community level,even when the COVID-19 prevalence is low.Overall,this study can be used as an important reference for contingency management of wastewater treatment plants and COVID-19 prevention and control departments of Wuhan. | Lu Zhao Evans Atoni Raphael Nyaruaba Yao Du Huaiyu Zhang Oscar Donde Doudou Huang Shuqi Xiao Nanjie Ren Teng Ma Zhu Shu Zhiming Yuan Lei Tong Han Xia | 2022 | Journal of Environmental Sciences2022，34，2： | 1 |
| 4 | SARS-CoV-2/COVID-19 laboratory biosafety practices and current molecular diagnostic tools显示文摘The ongoing severe acute respiratory syndrome coronavirus 2(SARS-CoV-2)/coronavirus disease 2019(COVID-19)pandemic has crippled several countries across the globe posing a serious global public health challenge.Despite the massive rollout of vaccines,molecular diagnosis remains the most important method for timely isolation,diagnosis,and control of COVID-19.Several molecular diagnostic tools have been developed since the beginning of the pandemic with some even gaining emergency use authorization from the United States(US)Food and Drug Administration for in vitro diagnosis of SARS-CoV-2.Herein,we discuss the working principles of some commonly used molecular diagnostic tools for SARS-CoV-2 including nucleic acid amplification tests,isothermal amplification tests,and rapid diagnostic tests.To ensure successful detection while minimizing the risk of cross-infection and misdiagnosis when using these diagnostic tools,laboratories should adhere to proper biosafety practices.Hence,we also present the common biosafety practices that may ensure the successful detection of SARS-CoV-2 from specimens while protecting laboratory workers and non-suspecting individuals from being infected.From this review article,it is clear that the SARS-CoV-2 pandemic has led to an increase in molecular diagnostic tools and the formation of new biosafety protocols that may be important for future and ongoing outbreaks. | Raphael Nyaruaba Caroline Mwaliko Wei Hong Patrick Amoth Hongping Wei | 2021 | Journal of Biosafety and Biosecurity2021，3，2： | 0 |
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