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| 1 | miR-23b promotes cutaneous wound healing through inhibition of the inflammatory responses by targeting ASK1显示文摘Wound healing is a complicated event that develops in three overlapping phases:inflammatory, proliferative,and remodeling.MicroRNAs (miRNAs)have been proved to play an important role in the healing process of skin trauma,and alteration of specific miRNA expression during different phases may be associated with abnormal wound healing.In this study,we determined the variation of miR-23b expression after trauma in normal mice and in cultured cells exposed to lipopolysaccharide.We further demonstrated that excessive miR-23b could significantly accelerate wound healing in vivo.Up-regulation of miR-23b decreases infiltration of inflammatory cells,as evidenced by pathologic staining.Meanwhile,miR-23b could significantly inhibit the expression of pro-inflammatory cytokines,including TNF-α,IL-1β,IL-6,and Ccl2,and significantly increase antiinflammatory factor IL-10.Furthermore,miR-23b could also promote α-SMA expression in a fiber pattern and increase the expression of Col1a1 and Col3a1.Importantly,we also showed that miR- 23b could inhibit inflammation to promote wound healing by targeting apoptotic signal-regulating kinase 1 (ASK1).Notably,knockdown of ASK1 could reduce inflammation factor expression in vitro.Together,our data reveal that miR-23b is a potent therapeutic agent for cutaneous wound healing that shortens the period of inflammatory responses and promotes keratinocyte migration for the re-epithelialization of wound sites. | Hongzhi Li Xiao Han Kuiyang Zuo Li Li Jieting Liu Xiaohuan Yuan Yongchao Shen Minglong Shao Daxin Pang Yanhui Chu Binghai Zhao | 2018 | Acta Biochimica et Biophysica Sinica2018，50，11： | 5 |
| 2 | Generation and characterization of stable pig pregastrulation epiblast stem cell lines显示文摘Pig epiblast-derived pluripotent stem cells are considered to have great potential and broad prospects for human therapeutic model developme nt and livestock breeding.Despite on going attempts since the 1990s,no stably defined pig epiblast-derived stem cell line has bee n established.Here,guided by in sights from a large-scale sin gle-cell tran scriptome an alysis of pig embryos from embryonic day(E)0 to E14,specifically,the tracing of pluripotency changes during epiblast development,we developed an in vitro culture medium for establishing and maintaining stable pluripotent stem cell lines from pig E10 pregastrulation epiblasts(pgEpiSCs).Enabled by chemical inhibition of WNT-related signaling in combination with growth factors in the FGF/ERK,JAK/STAT3,and Activin/Nodal pathways,pgEpiSCs maintain their pluripotency transcriptome features,similar to those of E10 epiblast cells,and normal karyotypes after more than 240 passages and have the potential to differentiate into three germ layers.Strikingly,ultradeep in situ Hi-C analysis revealed functional impacts of chromatin 3D-spatial associations on the transcriptional regulation of pluripote ncy marker genes in pgEpiSCs.I n practice,we con firmed that pgEpiSCs readily tolerate at least three rounds of successive gene editi ng and gene rated cloned gen e-edited live piglets.Our findings deliver on the long-a nticipated promise of pig pluripote nt stem cells and open new avenues for biological research,animal husbandry,and regenerative biomedicine. | Minglei Zhi Jinying Zhang Qianzi Tang Dawei Yu Shuai Gao Dengfeng Gao Pengliang Liu Jianxiong Guo Tang Hai Jie Gao Suying Cao Zimo Zhao Chongyang Li Xiaogang Weng Mengnan He Tianzhi Chen Yingjie Wang Keren Long Deling Jiao Guanglei Li Jiaman Zhang Yan Liu Yu Lin Daxin Pang Qianqian Zhu Naixin Chen Jingjing Huang Xinze Chen Yixuan Yao Jingcang Yang Zicong Xie Xianya Huang Mengxin Liu Ran Zhang Qiuyan Li Yiliang Miao Jianhui Tian Xingxu Huang Hongsheng Ouyang Bofeng Liu Wei Xie Qi Zhou Zhonghua Liu Caihong Zheng Mingzhou Li Jianyong Han | 2022 | Cell Research2022，32，4： | 3 |
| 3 | Vitamin C enhances in vitro and in vivo development of porcine somatic cell nuclear transfer embryos显示文摘 | Yongye Huang Xiaochun Tang Wanhua Xie Yan Zhou Dong Li Yang Zhou Jianguo Zhu Ting Yuan Liangxue Lai Daxin Pang Hongsheng Ouyang | 2011 | Biochemical and Biophysical Research Communications2011，，2： | 1 |
| 4 | Production of cloned transgenic cow expressing omega-3 fatty acids显示文摘 | Xia Wu Hongsheng Ouyang Biao Duan Daxin Pang Li Zhang Ting Yuan Lian Xue Daibang Ni Lei Cheng Shuhua Dong Zhuying Wei Lin Li Ming Yu Qing-Yuan Sun Da-Yuan Chen Liangxue Lai Yifan Dai Guang-Peng Li | 2012 | Transgenic Research2012，，3： | 1 |
| 5 | Scriptaid affects histone acetylation and the expression of development-related genes at different stages of porcine somatic cell nuclear transfer embryo during early development显示文摘Although the somatic cell nuclear transfer(SCNT) technique has been used extensively for cloning and generating transgenic pigs,the cloning efficiency is still very low.It has been proposed that the low efficiency of this technique is the result of incomplete epigenetic reprogramming and abnormal gene expression during early embryonic development.In this study,we investigate the effect of Scriptaid,a low-toxicity histone deacetylase inhibitor,on the developmental competence of porcine SCNT embryos.We found that treating SCNT embryos with 500 nmol/L Scriptaid for 15 h after activation significantly enhanced the blastocyst formation rate(27.7%) compared with the untreated group(control)(12.2%,P<0.05).Using an immunofluorescence technique to measure the average fluorescence intensity,we also found that treating SCNT embryos with Scriptaid increased the level of histone acetylation on histone H3 at lysine 14(acH3K14).Furthermore,treating embryos with Scriptaid increased the expression level of three genes that play important roles during embryonic development(Oct4,Klf4 at the blastocyst stage and Nanog at the 4-cell stage).Moreover,the expression level of the apoptosis-related gene Caspase-3 was significantly lower in the Scriptaid-treated SCNT embryos compared with the control SCNT embryos at the 4-cell and blastocyst stages.In conclusion,these results indicate that Scriptaid treatment improves the development and nuclear reprogramming of porcine SCNT embryos. | ZHOU Yan HUANG YongYe XIE WanHua SONG Qi JI Yuan ZHANG YanPing OUYANG HongSheng LAI LiangXue PANG DaXin TANG XiaoChun | 2013 | Chinese Science Bulletin2013，58，18： | 1 |
| 6 | Solvothermal Synthesis of Magnetic Fe3O4 Nanospheres and Their Efficiency in Photo-Fenton Degradation of Xylenol Orange显示文摘 | ZHENG Kai DI Mingyu ZHANG Jubo BAO Wenhui LIANG Daxin PANG Guangsheng FANG Zhenxing LI Chunyuan | 2017 | Chemical Research in Chinese Universities2017，33，4： | 0 |
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