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| 1 | The Genome of Artemisia annua Provides Insight into the Evolution of Asteraceae Family and Artemisinin Biosynthesis显示文摘Artemisia annua,通常已知的同样香甜的苦恼或 Qinghao,是到中国的一个灌木土著人并且长被用于药用的目的。A。annua 现在作为有势力抗疟药混合物的唯一的生来的来源全球性被栽培, artemisinin。这里,我们报导 A 的 1.74-gigabase 染色体的一个高质量的草稿集会。annua,高度异质接合,富于重复定序,并且包含 63 ? 226 编码蛋白质的基因,在定序的植物种类之中的最大的数字之一。我们发现了那,作为在 Asteraceae 的一些定序的染色体之一, A。annua 染色体包含对这大被子植物 clade 特定的很多基因。尤其是,扩大和编码涉及萜烯生合成的酶的基因的功能的多样化与 artemisinin biosynthetic 小径的进化一致。我们进一步由介绍那 A 的 transcriptome 揭示了。annua 发展了复杂 transcriptional 规章的网络位于 \O 下面 artemisinin 生合成。把转基因的 A 基于我们产生了的全面 genomic 和 transcriptomic 分析。artemisinin 高级的生产的 annua 线,它现在为大规模生产准备好了并且将从而帮助遇见增加 artemisinin 的全球需求的挑战。 | Qian Shen Lida Zhang Zhihua Liao Shengyue Wang Tingxiang Yan Pu Shi Meng Liu Xueqing Fu Qifang Pan Yuliang Wang Zongyou Lv Xu Lu Fangyuan Zhang Weimin Jiang Yanan Ma Minghui Chen Xiaolong Hao Ling Li Yueli Tang Gang Lv Yan Zhou Xiaofen Sun Peter E. Brodelius Jocelyn K.C. Rose Kexuan Tang | 2018 | Molecular Plant2018，11，6： | 47 |
| 2 | Environmental connections of novel avian-origin H7N9 influenza virus infection and virus adaptation to the human显示文摘A novel H7N9 influenza A virus has been discovered as the causative identity of the emerging acute respiratory infection cases in Shanghai,China.This virus has also been identified in cases of infection in the neighboring area Hangzhou City in Zhejiang Province.In this study,epidemiologic,clinical,and virological data from three patients in Hangzhou who were confirmed to be infected by the novel H7N9 influenza A virus were collected and analyzed.Human respiratory specimens and chicken feces from a contacted free market were tested for influenza virus by real-time reverse transcription PCR(RT-PCR) and sequencing.The clinical features of the three cases were similar featured with high fever and severe respiratory symptoms;however,only one of the patients died.A certain degree of diversity was observed among the three Hangzhou viruses sequenced from human samples compared with other reported H7N9 influenza A viruses.The sequences of the novel avian-origin H7N9 influenza viruses from Hangzhou City contained important amino acid substitutions related to human adaptation.One of the Hangzhou viruses had gained a novel amino acid substitution(Q226I) in the receptor binding region of hemagglutinin.More importantly,the virus sequenced from the chicken feces had a 627E substitution in the PB2 protein instead of the mammalian-adapted 627K substitution that was found in the PB2 proteins from the Hangzhou viruses from the three patients.Therefore,the newly-emerging H7N9 virus might be under adaptation pressure that will help it 'jump' from avian to human hosts.The significance of these substitutions needs further exploration,with both laboratory experiments and extensive field surveillance. | LI Jun YU XinFen PU XiaoYing XIE Li SUN YongXiang XIAO HaiXia WANG FenJuan DIN Hua WU Ying LIU Di ZHAO GuoQiu LIU Jun PAN JingCao | 2013 | Science China(Life Sciences)2013，56，6： | 32 |
| 3 | Current status of diagnosis and treatment of bladder cancer in China-Analyses of Chinese Bladder Cancer Consortium database显示文摘Objective:To investigate current status of diagnosis and treatment of bladder cancer in China.Methods:A database was generated by Chinese Bladder Cancer Consortium(CBCC).From January 2007 to December 2012,14,260 cases from 44 CBCC centers were included.Data of diagnosis,treatment and pathology were collected.Results:The average age was 63.5 year-old and most patients were male(84.3%).The most common histologic types were urothelial carcinoma(91.4%),adenocarcinoma(1.8%),and squamous carcinoma(1.9%).According to 1973 and 2004 WHO grading system,42.0%,41.0%,and 17.0% of patients were grade 1,2,and 3,and 16.0%,48.7%,and 35.3% of patients were papillary urothelial neoplasms of low malignant potential,low,and high grade,respectively.Non-muscle invasive bladder cancer(NMIBC)and muscle invasive bladder cancer(MIBC)were 25.2% and 74.1%,respectively(0.8% not clear).Carcinoma in situ was only 2.4%.Most patients were diagnosed by white-light cystoscopy with biopsy(74.3%).Fluorescence and narrow band imaging cystoscopy had additional detection rate of 1.0% and 4.0%,respectively.Diagnostic transurethral resection(TUR)provided detection rate of 16.9%.Most NMIBCs were treated with TUR(89.2%).After initial TUR,2.6%accepted second TUR,and 45.7%,69.9%,and 58.7% accepted immediate,induced,and maintenance chemotherapy instillation,respectively.Most MIBCs were treated with radical cystectomy(RC,59.7%).Laparoscopic RCs were 35.1%,while open RC 63.4%.Extended and standard pelvic lymph node dissection were 7% and 66%,respectively.Three most common urinary diversions were orthotopic neobladder(44%),ileal conduit(31%),and ureterocutaneostomy(23%).Only 2.3% of patients accepted neo-adjuvant chemotherapy and only 18%of T3 and T4 patients accepted adjuvant chemotherapy.Conclusion:Disease characteristics are similar to international reports,while differences of diagnosis and treatment exist.This study can provide evidences for revisions of the guideline on bladder cancer in China. | Kaiwen Li Tianxin Lin 无 Wei Xue Xin Mu Enci Xu Xu Yang Fubao Chen Guangyong Li Lulin Ma Guoliang Wang Chaozhao Liang Haoqiang Shi Ming Li Mao Tang Xueyi Xue Yisong Lv Yaoliang Deng Chengyang Li Zhiwen Chen Xiaozhou Zhou Fengshuo Jin Xudong Liu Jinxin Wei Lei Shi Xin Gou Weiyang He Liqun Zhou Lin Cai Baiye Jin Guanghou Fu Xiangbo Kong Hongyan Sun Ye Tian Lang Feng Tiejun Pan Yiyi Wu Dongwen Wang Hailong Hao Benkang Shi Yaofeng Zhu Qiang Wei Ping Han Changli Wu Dawei Tian Zhangqun Ye Zheng Liu Zhiping Wang Junqiang Tian Lin Qi Minfeng Chen Wei Li Jinchun Qi Gongxian Wang Longlong Fu Zhaolin Sun Guangheng Luo Zhoujun Shen Zhaowei Zhu Jinchun Xing Zhun Wu Dong Wei Xin Chen Yanqun Na Hongfeng Guo Chunxi Wang Zhihua Lu Chuize Kong Yang Liu Jin Yang Jianyun Hu Xin Gao Jielin Li Changjun Yin Pu Li Shan Chen Zhen Du Jiongming Li Yongji Yan Xu Zhang Shuang Huang Fangjian Zhou Zhiling Zhang Yinghao Sun Shuxiong Zeng Song Cen Jiaquan Zhou Hanzhong Li Jin Wen Jian Huang | 2015 | Asian Journal of Urology2015，2，2： | 21 |
| 4 | Concurrent intracranial and extracranial artery stenosis and the prognosis of transient ischaemic symptoms or imaging-negative ischaemic stroke显示文摘Background and purpose Transient ischaemic attack(TIA),transient symptoms with infarction(TSI)and diffusion-weighted imaging(DWI)-negative acute ischaemic stroke(AIS)share similar aetiologies but are considered to have a rather benign prognosis.We intended to investigate the association between intracranial atherosclerotic stenosis(ICAS),extracranial atherosclerotic stenosis(ECAS)and the prognosis of patients with TIA,TSI and DWI-negative AIS.Methods Clinical and imaging data of eligible participants were derived from the Chinese Intracranial Atherosclerosis study,according to symptom duration,acute infarction on DWI and discharge diagnosis.Based on the severity and location of arterial atherosclerosis,we categorised the study population into four groups:no or<50% ICAS and no ECAS;≥50% ICAS but no ECAS;no or<50%ICAS with ECAS;and concurrent≥50% ICAS and ECAS.Using multivariable Cox regression models,we analysed the relationship between the severity and distribution of large artery atherosclerosis and the prognosis of TIA,TSI and DWI-negative AIS.Results A total of 806 patients were included,67.3% of whom were male.The median age of the study participants was 63 years.Patients in the concurrent≥50% ICAS and ECAS subgroup had both a significantly higher 1-year recurrence rate(adjusted HR 3.4(95%CI 1.15 to 10.04),p=0.027)and a higher risk of composite vascular events(adjusted HR 3.82(95%CI 1.50 to 9.72),p=0.005).Conclusions Concurrent ICAS and ECAS is associated with a higher possibility of 1-year recurrent stroke or composite vascular events.Large artery evaluation is necessary to assess patients with transient ischaemic symptoms or DWI-negative AIS.Progress in shortening the time interval between symptom onset and large vessel evaluation is needed. | Yue Suo Jing Jing Yuesong Pan Weiqi Chen Hongyu Zhou Hao Li Yuehua Pu Liping Liu Xingquan Zhao Yilong Wang Xia Meng Yongjun Wang | 2021 | Stroke & Vascular Neurology2021，6，1： | 10 |
| 5 | Endovascular treatment with or without intravenous alteplase for acute ischaemic stroke due to basilar artery occlusion显示文摘Background and purpose It remains controversial if endovascular treatment(EVT)can improve the outcome of patients with acute basilar artery occlusion(BAO).This study aims to compare the functional outcomes between EVT with and without intravenous thrombolysis(IVT)first in patients who had acute ischaemic stroke(AIS)due to BAO.Methods Patients who had AIS with BAO who underwent EVT within 24 hours of onset were enrolled in this multicentre cohort study,and the efficacy and safety were compared between IVT+EVT and direct EVT.The primary outcome was 90-day functional independence.All outcomes were assessed with adjusted OR(aOR)from the multivariable logistic regression.In addition,a meta-analysis was performed on all recently published pivotal studies on functional independence after EVT in patients with BAO.Results Of 310 enrolled patients with BAO,241(78%)were treated with direct EVT and 69(22%)with IVT+EVT.Direct EVT was associated with a worse functional outcome(aOR,0.46(95%CI 0.24 to 0.85),p=0.01).IVT+EVT was associated with a lower percentage of patients who needed≥3 passes of stent retriever(10.14%vs 20.75%).The meta-analysis regression revealed a potential positive correlation between bridging with IVT first and functional independence(r=0.14(95%CI 0.05 to 0.24),p<0.01).Conclusions This study showed that compared with direct EVT,EVT with IVT first was associated with better functional outcomes in patients with BAO treated within 24 hours of onset.The meta-analysis demonstrated similar favourable efficacy of IVT first followed by EVT in patients with BAO. | Ximing Nie David Wang Yuehua Pu Yufei Wei Qixuan Lu Hongyi Yan Xin Liu Lina Zheng Jingyi Liu Xinxuan Yang Yarong Ding Dacheng Liu Wanying Duan Zhe Zhang Zhonghua Yang Miao Wen Weibin Gu Xinyi Hou Xinyi Leng Yuesong Pan Zhongrong Miao Liping Liu | 2022 | Stroke & Vascular Neurology2022，7，3： | 10 |
| 6 | Recently developed strategies to restrain dendrite growth of Li metal anodes for rechargeable batteries显示文摘Lithium metal has been regarded as one of the most promising anode materials for high-energy-density batteries due to its extremely high theoretical gravimetric capacity of 3860 mAh·g^-1 along with its low electrochemical potential of-3.04 V.Unfortunately,uncontrollable Li dendrite growth and repetitive destruction/formation of the solid electrolyte interphase layer lead to poor safety and low Coulombic efficiencies(CEs)for long-term utilization,which largely restricts the practical applications of lithium metal anode.In this review,we comprehensively summarized important progresses achieved to date in suppressing Li dendrite growth.Strategies for protection of Li metal anodes include designing porous structured hosts,fabricating artificial solid electrolyte interface(SEI)layers,introducing electrolyte additives,using solid-state electrolytes and applying external fields.The protection of Li metal anodes can be achieved by regulating the stripping and deposition behaviours of Li ions.Finally,the challenges remaining for lithium metal battery systems and future perspectives for Li metal anodes in practical applications are outlined,which are expected to shed light on future research in this field. | Kai-Chao Pu Xin Zhang Xiao-Lei Qu Jian-Jiang Hu Hai-Wen Li Ming-Xia Gao Hong-Ge Pan Yong-Feng Liu | 2020 | Rare Metals2020，39，6： | 8 |
| 7 | Functional Fibers and Functional Fiber‑Based Components for High‑Power Lasers显示文摘The success of high-power fiber lasers is fueled by maturation of active and passive fibers,combined with the availability of high-power fiber-based components.In this contribution,we first overview the enormous potential of rare-earth doped fibers in spectral coverage and recent developments of key fiber-based components employed in high-power laser systems.Subsequently,the emerging functional active and passive fibers in recent years,which exhibit tremendous advantages in balancing or mitigating parasitic nonlinearities hindering high-power transmission,are outlined from the perspectives of geo-metric and material engineering.Finally,novel functional applications of conventional fiber-based components for nonlinear suppression or spatial mode selection,and correspondingly,the high-power progress of function fiber-based components in power handling are introduced,which suggest more flexible controllability on high-power laser operations. | Xiao Chen Tianfu Yao Liangjin Huang Yi An Hanshuo Wu Zhiyong Pan Pu Zhou | 2023 | Advanced Fiber Materials2023，5，1： | 5 |
| 8 | The treatment of black-odorous water using tower bipolar electro-flocculation including the removal of phosphorus,turbidity,sulfion,and oxygen enrichment显示文摘The large amount of municipal wastewater discharged into urban rivers sometimes exceeds the rivers’self-purification capacity leading to black-odorous polluted water.Electro-flocculation has emerged as a powerful remediation technology.Electro-flocculation in a bubble column tower with a bipolar electrode(BPE)was tested in an attempt to overcome the high resistance and weak gas-floatation observed with a monopolar electrode(MPE)in treating such water.The BPE reactor tested had a Ti/Ta2O_(5)-IrO_(2)anode and a graphite cathode with an iron or aluminum bipolar electrode suspended between them.It was tested for its ability to reduce turbidity,phosphate and sulphion and to increase the concentration of dissolved oxygen.The inclusion of the bipolar electrode was found to distinctly improved the system’s conductivity.The system’s electro-flocculation and electrical floatation removed turbidity,phosphate and sulphion completely,and the dissolved oxygen level improved from 0.29 to 6.28 mg/L.An aluminum bipolar electrode performed better than an iron one.Changes in the structure of the microbial community confirmed a significant improvement in water quality. | Huan He Qinjin Yu Chaochao Lai Chen Zhang Muhan Liu Bin Huang Hongping Pu Xuejun Pan | 2021 | Frontiers of Environmental Science & Engineering2021，15，2： | 5 |
| 9 | Establishment and validation of a computer-assisted colonic polyp localization system based on deep learning显示文摘BACKGROUND Artificial intelligence in colonoscopy is an emerging field,and its application may help colonoscopists improve inspection quality and reduce the rate of missed polyps and adenomas.Several deep learning-based computer-assisted detection(CADe)techniques were established from small single-center datasets,and unrepresentative learning materials might confine their application and generalization in wide practice.Although CADes have been reported to identify polyps in colonoscopic images and videos in real time,their diagnostic performance deserves to be further validated in clinical practice.AIM To train and test a CADe based on multicenter high-quality images of polyps and preliminarily validate it in clinical colonoscopies.METHODS With high-quality screening and labeling from 55 qualified colonoscopists,a dataset consisting of over 71000 images from 20 centers was used to train and test a deep learning-based CADe.In addition,the real-time diagnostic performance of CADe was tested frame by frame in 47 unaltered full-ranged videos that contained 86 histologically confirmed polyps.Finally,we conducted a selfcontrolled observational study to validate the diagnostic performance of CADe in real-world colonoscopy with the main outcome measure of polyps per colonoscopy in Changhai Hospital.RESULTS The CADe was able to identify polyps in the test dataset with 95.0%sensitivity and 99.1%specificity.For colonoscopy videos,all 86 polyps were detected with 92.2%sensitivity and 93.6%specificity in frame-by-frame analysis.In the prospective validation,the sensitivity of CAD in identifying polyps was 98.4%(185/188).Folds,reflections of light and fecal fluid were the main causes of false positives in both the test dataset and clinical colonoscopies.Colonoscopists can detect more polyps(0.90 vs 0.82,P<0.001)and adenomas(0.32 vs 0.30,P=0.045)with the aid of CADe,particularly polyps<5 mm and flat polyps(0.65 vs 0.57,P<0.001;0.74 vs 0.67,P=0.001,respectively).However,high efficacy is not realized in colonoscopies with inadequate bowel preparation and withdrawal time(P=0.32;P=0.16,respectively).CONCLUSION CADe is feasible in the clinical setting and might help endoscopists detect more polyps and adenomas,and further confirmation is warranted. | Sheng-Bing Zhao Wei Yang Shu-Ling Wang Peng Pan Run-Dong Wang Xin Chang Zhong-Qian Sun Xing-Hui Fu Hong Shang Jian-Rong Wu Li-Zhu Chen Jia Chang Pu Song Ying-Lei Miao Shui-Xiang He Lin Miao Hui-Qing Jiang Wen Wang Xia Yang Yuan-Hang Dong Han Lin Yan Chen Jie Gao Qian-Qian Meng Zhen-Dong Jin Zhao-Shen Li Yu Bai | 2021 | World Journal of Gastroenterology2021，27，31： | 5 |
| 10 | Precipitation Behavior and Microstructural Evolution of Ferritic Ti–V–Mo Complex Microalloyed Steel显示文摘Precipitation behavior of(Ti,V,Mo)C and microstructural evolution of the ferritic Ti–V–Mo complex microalloyed steel were investigated through changing coiling temperature(CT).It is demonstrated that the strength of the Ti–V–Mo microalloyed steel can be ascribed to the combination of grain refinement hardening and precipitation hardening.The variation of hardness(from 318 to 415 HV,then to 327 HV) with CT(from 500 to 600–625 °C,then to 700 °C) was attributed to the changes of volume fraction and particle size of(Ti,V,Mo)C precipitates.The optimum CT was considered as 600–625 °C,at which the maximum hardness value(415 HV) can be obtained.It was found that the atomic ratios of Ti,V and Mo in(Ti,V,Mo)C carbides were changed as the CT increased.The precipitates with the size of \ 10 nm were the V-rich particles at higher CT of 600 and 650 °C,while the Ti-rich particles were observed at lower CT of 500 and 550 °C.Theoretical calculations indicated that the maximum nucleation rate of(Ti,V,Mo)C in ferrite matrix occurred around 630 °C,which was consistent with the 625 °C obtained from experiment results. | Ke Zhang Hui Wang Xin-Jun Sun Feng-Li Sui Zhao-Dong Li En-Xiang Pu Zheng-Hai Zhu Zhen-Yi Huang Hong-Bo Pan Qi-Long Yong | 2018 | Acta Metallurgica Sinica(English Letters)2018，31，9： | 4 |
| 11 | A novel laser shock post-processing technique on the laser-induced damage resistance of 1ωHfO_(2)/SiO_(2)显示文摘The laser shock processing implemented by a laser-induced high-pressure plasma which propagates into the sample as a shockwave is innovatively applied as a post-processing technique on HfO_(2)/SiO_(2) multilayer coatings for the first time.The pure mechanical post-processing has provided evidence of a considerable promotion effect of the laser-induced damage threshold,which increased by a factor of about 4.6 with appropriate processing parameters.The promotion mechanism is confirmed to be the comprehensive modification of the intrinsic defects and the mechanical properties,which made the applicability of this novel post-processing technique on various types of coatings possible.Based on experiments,an interaction equation for the plasma pressure is established,which clarifies the existence of the critical pressure and provides a theoretical basis for selecting optimal processing parameters.In addition to the further clarification of the underlying damage mechanism,the laser shock post-processing provides a promising technique to realize the comprehensive and effective improvement of the laser-induced damage resistance of coatings. | Tangyang Pu Wenwen Liu Yueliang Wang Xiaoming Pan Leiqing Chen and Xiaofeng Liu | 2021 | High Power Laser Science and Engineering2021，9，2： | 4 |
| 12 | Cyclin-dependent Kinase 18 Promotes Oligodendrocyte Precursor Cell Differentiation through Activating the Extracellular Signal-Regulated Kinase Signaling Pathway显示文摘The correct differentiation of oligodendrocyte precursor cells(OPCs) is essential for the myelination and remyelination processes in the central nervous system.Determining the regulatory mechanism is fundamental to the treatment of demyelinating diseases. By analyzing the RNA sequencing data of different neural cells, we found that cyclin-dependent kinase 18(CDK18) is exclusively expressed in oligodendrocytes. In vivo studies showed that the expression level of CDK18 gradually increased along with myelin formation during development and in the remyelination phase in a lysophosphatidylcholine-induced demyelination model, and was distinctively highly expressed in oligodendrocytes. In vitro overexpression and interference experiments revealed that CDK18 directly promotes the differentiation of OPCs, without affecting their proliferation or apoptosis. Mechanistically, CDK18 activated the RAS/mitogen-activated protein kinase kinase1/extracellular signal-regulated kinase pathway, thus promoting OPC differentiation. The results of the present study suggest that CDK18 is a promising cell-type specific target to treat demyelinating disease. | Yuchen Pan Zeping Jiang Dingya Sun Zhenghao Li Yingyan Pu Dan Wang Aijun Huang Cheng He Li Cao | 2019 | Neuroscience Bulletin2019，35，5： | 4 |
| 13 | A review on novel activation strategy on carbonaceous materials with special morphology/texture for electrochemical storage显示文摘Various novel carbonaceous materials including carbon nanotubes,nano-onions,carbon microspheres,graphene nanosheets,and carbon microfibers with unique properties,such as tunable surface area and pore size,high chemical stability,cost-effective and facile preparation,have attracted enormous interest for many applications.Also essential,the activation processes play a critical role to achieve these valuable properties.In this review,we provide a thorough analysis of the emerging nano-and microscopic carbon species with special morphology/textures and currently available types of chemical activation agents,and novel activation strategy to enhance electrochemical performance of activated carbon material in electrical energy storage devices including supercapacitor and alkaline ions batteries.A particular emphasis is set on recent advance in activated carbon materials with special morphology/textures for supercapacitors and sodium ion batteries. | Yueming Li Ziyan Pu Qimeng Sun Ning Pan | 2021 | Journal of Energy Chemistry2021，30，9： | 3 |
| 14 | Transverse mode instability mitigation in a high-power confined-doped fiber amplifier with good beam quality through seed laser control显示文摘In this work,a confined-doped fiber with the core/inner-cladding diameter of 40/250μm and a relative doping ratio of 0.75 is fabricated through a modified chemical vapor deposition method combined with the chelate gas deposition technique,and subsequently applied in a tandem-pumped fiber amplifier for high-power operation and transverse mode instability(TMI)mitigation.Notably,the impacts of the seed laser power and mode purity are preliminarily investigated through comparative experiments.It is found that the TMI threshold could be significantly affected by the seed laser mode purity.The possible mechanism behind this phenomenon is proposed and revealed through comprehensive comparative experiments and theoretical analysis.Finally,a maximum output power of 7.49 kW is obtained with the beam quality factor of approximately 1.83,which is the highest output power ever reported in a forward tandem-pumped confined-doped fiber amplifier.This work could provide a good reference and practical solution to improve the TMI threshold and realize high-power high-brightness fiber lasers. | Hanshuo Wu Haobo Li Yi An Ruixian Li Xiao Chen Hu Xiao Liangjin Huang Huan Yang Zhiping Yan Jinyong Leng Zhiyong Pan Pu Zhou | 2022 | High Power Laser Science and Engineering2022，10，6： | 3 |
| 15 | Diagnosis of intellectual disability/global developmental delay via genetic analysis in a central region of China显示文摘Background:Advanced technology has become a valuable tool in etiological studies of intellectual disability/global developmental delay(ID/GDD).The present study investigated the role of genetic analysis to confirm the etiology in ID/GDD patients where the cause of the disease was uncertain in central China.Methods:We evaluated 1051 ID/GDD children aged 6 months to 18 years from March 2009 to April 2017.Data concerning basic clinical manifestations were collected,and the method of etiology confirmation was recorded.Genome-wide copy number variations(CNVs)detection and high-throughput sequencing of exons in the targeted regions was performed to identify genetically-based etiologies.We compared the incidence of different methods used to confirm ID/GDD etiology among groups with differing degrees of ID/GDD using the Chi-square or Fisher exact probability test.Results:We recruited 1051 children with mild(367,34.9%),moderate(301,28.6%),severe(310,29.5%),and profoundly severe(73,6.9%)ID/GDD.The main causes of ID/GDD in the children assessed were perinatal factors,such as acquired brain injury,as well as single gene imbalance and chromosomal gene mutation.We identified karyotype and/or CNVs variation in 46/96(47.9%)of cases in severe ID/GDD patients,which was significantly higher than those with mild and moderate ID/GDD of 34/96(35.4%)and 15/96(15.6%),respectively.A total of 331/536(61.8%)patients with clear etiology have undergone genetic analysis while 262/515(50.9%)patients with unclear etiology have undergone genetic analysis(x^2=12.645,P<0.001).Gene structure variation via karyotype analysis and CNV detection increased the proportion of children with confirmed etiology from 51.0%to 56.3%,and second-generation high-throughput sequencing dramatically increased this to 78.9%.Ten novel mutations were detected,recessive mutations in X-linked genes(ATPase copper transporting alpha and bromodomain and WD repeat domain containing 3)and dominant de novo heterozygous mutations in X-linked genes(cyclin-dependent kinase like 5,protocadherin 19,IQ motif and Sec7 domain 2,and methyl-CpG binding protein 2)were reported in the study.Conclusions:The present study indicates that genetic analysis is an effective method to increase the proportion of confirmed etiology in ID/GDD children and is highly recommended,especially in ID/GDD children with uncertain etiology. | Li-Hong Liao Chen Chen Jing Peng Li-Wen Wu Fang He Li-Fen Yang Ci-Liu Zhang Guo-Li Wang Pan Peng Yu-Ping Ma Pu Miao Fei Yin | 2019 | Chinese Medical Journal2019，，13： | 3 |
| 16 | Enhanced pressor response to acute Ang II infusion in mice lacking membrane-associated prostaglandin E2 synthase-1显示文摘 | Dong-juan ZHANG Li-hong CHEN Ya-hua ZHANG Guang-rui YANG Dou DOU Yuan-sheng GAO Xiao-yan ZHANG Xiao-mu KONG Pan ZHAO Dan PU Ming-fen WEI Matthew-D BREYER You-fei GUAN | 2010 | Acta Pharmacologica Sinica2010，31，10： | 3 |
| 17 | Study protocol:A cross-sectional study on psychology and behavior investigation of Chinese residents,PBICR显示文摘Objective:This study protocol identifies the basic research route and framework of psychological and behavioral surveys among Chinese residents,aims establishing a database through a multicenter,large-sample cross-sectional survey in China to provide strong data support for research development in various fields and a more comprehensive and systematic understanding of the physical and mental health status of the public.Method:The study was conducted from June 20,2022 to August 31,2022,using stratified sampling and quota sampling methods,a total of 148 cities,202 districts and counties,390 townships/towns/sub-districts,and 780 communities/villages(excluding Hong Kong,Macao,and Taiwan)from 23 provinces,5 autonomous regions,and 4 municipalities directly under the central government in China were selected.The questionnaire was distributed one-on-one and face-to-face to the public by trained investigators,and the questionnaire included eight aspects:personal basic information,personal health status,family basic information,social environment in which they were located,psychological level scale,behavioral level scale,other scales,and attitude towards social hot issues.Data analysis will be performed after questionnaire return.Results:Data collection is ongoing.These findings will support physical and mental health research and strategy development in China and even globally,guiding policy-makers and health care organizations to reform their programs to ensure the best interests of residents and their families. | Yu-Jia Wang Ayidana Kaierdebieke Si-Yuan Fan Rui-Feng Zhang Meng-Jie Huang Han Li Xiao-Nan Sun Qi-Yu Li Wen-Jia Meng Wen-Yu Wu Zhi Lin Jing-Yao Liu Xin-Pei Wang Yun-Chou Wu Jing-Qi Tang Yi-Ke Sun Ke Chen Pu Ge Wai-Kit Ming Casper Zhang Zheng Feei Ma Lei Feng Xin-Yue Zhang Yu-Yao Niu Yu-Pei Yan Yi-Le Jin Guang-Ze Gao Shuang Dai Yu-Liu Li Yue Tan Yi-Wei Wu Qi Zhang Gui Gui Xian-Li Pan Yan-Ming Liao Xian-Qi Zhao Ya-Ting Zhang Hui-Yi Chen Yue-Wei Qiu Xiao-Min Fu Jiang-Ling Zhou Dan Li Ke-Han Li Meng-Wei Xu Zhe Wang Yue-Qi Wang Yong Ma Xin-Ying Sun Yi-Bo Wu | 2022 | Psychosomatic Medicine Research2022，4，3： | 3 |
| 18 | Morphological evaluation of retinal ganglion cells expressing the L132C/T159C ChR2 mutant transgene in young adult cynomolgus monkeys显示文摘To characterize recombinant AAV2(rAAV2)-mediated expression of L132 C/T159 C ChR2 mutant in retinal ganglion cells(RGCs)of young adult cynomolgus monkeys, rAAV2 vectors carrying a fusion construct of the ChR2 mutant and GFP(ChR2-GFP) were delivered to the vitreous chamber by intravitreal injection. Expression patterns of the ChR2 mutant in RGCs were examined by immunohistochemical methods three months after injection. The RNA-binding protein with multiple splicing(RBPMS) was used as an RGC specific marker to differentiate RGCs from other retinal neurons and non-neuronal cells. The numbers of RBPMS+and GFP+ double-labeled RGCs in the central foveal varied with the eccentricity. The expression peaked within 100 μm from the edge of the foveola and drastically decreased to a single superficial RGC layer approximately 300 μm from the edge. On average,the ratio of the double-labeled RGCs versus RBPMS+ RGCs approached 0.32±0.15(n=14 fields) at the central foveal region(0.1 to 0.53 mm). We observed that the ratio reached 0.78±0.16(n=21 fields) at peripheral retinal locations(eccentricity >7 mm). This investigation demonstrates that RBPMS could serve as a valuable RGC specific marker for future investigations in this field. | Wenyao Wang Yan Nan Zhuo-Hua Pan Mingliang Pu | 2017 | Science China(Life Sciences)2017，60，11： | 2 |
| 19 | Swansea criteria score in acute fatty liver of pregnancy显示文摘To the Editor:Acute fatty liver of pregnancy(AFLP)is an uncommon but potentially fatal disease unique to pregnancy that typically occurs in the third trimester and is one of the leading causes of maternal mortality in critically ill patients.^([1])The Swansea diagnostic criteria have been widely used in the diagnosis of AFLP worldwide since their proposal.The Swansea criteria include clinical symptoms and laboratory tests,imaging,and pathological examination results. | Jianbo Tan Fei Hou Haofeng Xiong Lin Pu Pan Xiang Chuansheng Li | 2022 | Chinese Medical Journal2022，，7： | 2 |
| 20 | Assessment of ischemia-modified albumin levels for emergency room diagnosis of acute coronary syndrome显示文摘 | Xiao-li Shen Cai-jing Lin Li-li Han Lin Lin Leng Pan Xiao-dong Pu | 2010 | International Journal of Cardiology2010，，3： | 2 |
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