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| 1 | An infectious disease of ducks caused by a newly emerged Tembusu virus strain in mainland China显示文摘 | Pixi Yan Youshu Zhao Xu Zhang Dawei Xu Xiaoguang Dai Qiaoyang Teng Liping Yan Jiewen Zhou Xiwen Ji Shumei Zhang Guangqing Liu Yanjun Zhou Yoshihiro Kawaoka Guangzhi Tong Zejun Li | 2011 | Virology2011，，1： | 5 |
| 2 | Mouse knockout models reveal largely dispensable but context-dependent functions of IncRNAs during development显示文摘 | Xue Han Sai Luo Guangdun Peng Yuyang Lu Guizhong Cui Lichao Liu Pixi Yan Yafei Yin Wei Liu Ran Wang Jiejie Zhang Shanshan Ai Zai Chang Jie Na Aibin He Naihe Jing Xiaohua Shen | 2018 | Journal of Molecular Cell Biology2018，10，2： | 3 |
| 3 | LncRNA Platr22 promotes super-enhancer activity and stem cell pluripotency显示文摘Super-enhancers(SEs)comprise large clusters of enhancers,which are co-occupied by multiple lineage-specific and master tran-scription factors,and play pivotal roles in regulating gene expression and cell fate determination.However,it is still largely un-known whether and how SEs are regulated by the noncoding portion of the genome.Here,through genome-wide analysis,wefound that tpng noncoding RNA(IncRNA)genes preferentially lie next to SEs.In mouse embryonic stem cells(mESCs),depletionof$E-associated IlncRNA transcripts dysregulated the activity of their nearby SEs.Specifically,we revealed a critical regulatoryrole of the IncRNA gene Platr22 in modulating the activity of a nearby SE and the expression of the nearby pluripotency regulatorZFP281.Through these regulatory events,Platr22 contributes to pluripotency maintenance and proper differentiation of mESCs.Mechanistically,Platr22 transcripts coat chromatin near the SE region and interact with DDX5 and hnRNP-L.DDX5 further recruitsp300 and other factors related to active transcription.We propose that these factors assemble into a transcription hub,thus pro-moting an open and active epigenetic chromatin state.0ur study highlights an unanticipated role for a class of lncRNAs in epige-netically controlling the activity and vulnerability to perturbation of nearby SEs for cell fate determination. | Pixi Yan Yuyang Lu Jing Niu Juntao Gao Michael Q.Zhang Yafei Yin Xiaohua Shen | 2021 | Journal of Molecular Cell Biology2021，13，4： | 2 |
| 4 | An infectious disease of ducks caused by a newly emerged Tembusu virus strain in mainland China显示文摘 | Pixi Yan Youshu Zhao Xu Zhang | 2011 | Virology2011，417，： | 1 |
| 5 | Establishing a TaqMan-Based Real-Time PCR Assay for the Rapid Detection and Quantification of the Newly Emerged Duck Tembusu Virus显示文摘 | Liping Yan Pixi Yan Jiewen Zhou | 2011 | Virology Journal2011，8，： | 1 |
| 6 | An in-fectious disease of ducks caused by a newly e-merged Tembusu virus strain in mainland China显示文摘 | Yan pixi Zhao youshu Zhang xu | | 0，，： | 1 |
| 7 | Homotypic clustering of L1 and B1/Alu repeats compartmentalizes the 3D genome显示文摘Organization of the genome into euchromatin and heterochromatin appears to be evolutionarily conserved and relatively stable during lineage differentiation.In an effort to unravel the basic principle underlying genome folding,here we focus on the genome itself and report a fundamental role for L1(LINE1 or LINE-1)and B1/Alu retrotransposons,the most abundant subclasses of repetitive sequences,in chromatin compartmentalization.We find that homotypic clustering of L1 and B1/Alu demarcates the genome into grossly exclusive domains,and characterizes and predicts Hi-C compartments.Spatial segregation of L1-rich sequences in the nuclear and nucleolar peripheries and B1/Alu-rich sequences in the nuclear interior is conserved in mouse and human cells and occurs dynamically during the cell cycle.In addition,de novo establishment of L1 and B1 nuclear segregation is coincident with the formation of higher-order chromatin structures during early embryogenesis and appears to be critically regulated by L1 and B1 transcripts.Importantly,depletion of L1 transcripts in embryonic stem cells drastically weakens homotypic repeat contacts and compartmental strength,and disrupts the nuclear segregation of L1-or B1-rich chromosomal sequences at genome-wide and individual sites.Mechanistically,nuclear co-localization and liquid droplet formation of L1 repeat DNA and RNA with heterochromatin protein HP1αsuggest a phase-separation mechanism by which L1 promotes heterochromatin compartmentalization.Taken together,we propose a genetically encoded model in which L1 and B1/Alu repeats blueprint chromatin macrostructure.Our model explains the robustness of genome folding into a common conserved core,on which dynamic gene regulation is overlaid across cells. | J.Yuyang Lu Lei Chang Tong Li Ting Wang Yafei Yin Ge Zhan Xue Han Ke Zhang Yibing Tao Michelle Percharde Liang Wang Qi Peng Pixi Yan Hui Zhang Xianju Bi Wen Shao Yantao Hong Zhongyang Wu Runze Ma Peizhe Wang Wenzhi Li Jing Zhang Zai Chang Yingping Hou Bing Zhu Miguel Ramalho-Santos Pilong Li Wei Xie Jie Na Yujie Sun Xiaohua Shen | 2021 | Cell Research2021，31，6： | 1 |
| 8 | Establishing a TaqMan-based real-time PCR assay for the rapid detection and quantification of the newly emerged duck Tembusu virus显示文摘 | Yan Liping Yan Pixi Zhou Jiewen | 2011 | Virol J2011，8，1： | 1 |
| 9 | Comparison of real-time reverse transcription loop-mediated isothermal amplification and real-time reverse transcription polymerase chain reaction for duck Tembusu virus显示文摘 | Liping Yan Shan Peng Pixi Yan Jiewen Zhou Qiaoyang Teng Guoxin Li Xuesong Li Zejun Li | 2012 | Journal of Virological Methods (-)2012，，1： | 1 |
| 10 | An infectious disease of ducks caused by a newly emerged tembusu virus strain in mainland China 显示文摘 | Yan Pixi Zhao Youshu Zhang Xu | 2011 | Virology2011，417，1： | 1 |
| 11 | An infectious disease of ducks caused by a newly emerged Tembusu virus strain in mainland China显示文摘 | Pixi Yan Youshu Zhao Xu Zhang et al | 2011 | Virology2011，417，1： | 1 |
| 12 | 反义长链非编码RNA调控基因表达和多能干细胞分化显示文摘高等真核生物基因组的广泛转录产生了大量的长链非编码RNA(lncRNA),它们不具有编码蛋白的功能。目前人们对它们的了解还非常有限,lncRNA被认为是生物学中的暗物质。LncRNA的功能分类及预测,是非编码RNA领域一直追寻和探索的重要问题,它对于认识非编码RNA生物学功能和存在的意义具有重要作用。 | Sai Luo J.Yuyang Lu Lichao Liu Yafei Yin Chunyan Chen Xue Han Bohou Wu Ronggang Xu Wei Liu Pixi Yan Wen Shao Zhi Lu Haitao Li Jie Na Fuchou Tang Jianlong Wang Yong E.Zhang 沈晓骅 | 2017 | 科学新闻2017，19，4： | 0 |
| 13 | Tn5-FISH,a novel cytogenetic method to image chromatin interactions with sub-kilobase resolution显示文摘There is an increasing interest in understanding how three-dimensional(3D)organization of the genome is regulated.Different strategies have been employed to identify genome-wide chromatin interactions.However,due to current limitations in resolving genomic contacts,visualization and validation of these genomic loci with sub-kilobase resolution remain unsolved to date.Here,we describe Tn5 transposase-based Fluorescencein situhybridization(Tn5-FISH),a PCR-based,cost-effective imaging method,which can co-localize the genomic loci with sub-kilobase resolution,dissect genome architecture,and verify chromatin interactions detected by chromatin configuration capture(3C)-derived methods.To validate this method,short-range interactions in keratin-encoding gene(KRT)locus in topologically associated domain(TAD)were imaged by triple-color Tn5-FISH,indicating that Tn5-FISH is very useful to verify short-range chromatin interactions inside the contact domain and TAD.Therefore,Tn5-FISH can be a powerful molecular tool for the clinical detection of cytogenetic changes in numerous genetic diseases such as cancers. | Jing Niu Xu Zhang Li Guipeng Pixi Yan Qing Yan Qionghai Dai Dayong Jin Xiaohua Shen Jichang Wang Michael QZhang Juntao Gao | 2020 | Journal of Genetics and Genomics2020，47，12： | 0 |
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