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| 1 | Generation of iPSCs from mouse fibroblasts with a single gene, Oct4, and small molecules显示文摘由病毒的 transduction 的四抄写因素 Oct4, Klf4, Sox2 和 c-Myc 的介绍能导致体的房间的 reprogramming 进导致的 pluripotent 干细胞(iPSCs ) ,但是 iPSCs 的使用被病毒的交货系统的使用妨碍。导致化学药品的 reprogramming 把一条新奇途径提供给没有任何病毒的基于向量的基因修正,产生 iPSCs。尽管,以前的报告证明几个小分子能代替一些 reprogramming 因素至少二个抄写因素, Oct4 和 Klf4,仍然被要求从老鼠产生 iPSCs 胚胎的成纤维细胞。这里,我们识别特定的化学联合,它是足够的面对一个单个抄写因素从胚胎的老鼠和成年成纤维细胞允许 reprogramming, Oct4 在 20 天以内,代替 Sox2, Klf4 和 c-Myc。用这个处理产生的 iPSCs 类似于胚胎的干细胞以全球基因表示介绍的老鼠,在 vitro 并且在 vivo 的 epigenetic 地位和 pluripotency。我们也发现那 8 天 Oct4 正式就职是足够的面对小分子启用导致 Oct4 的 reprogramming,它建议 reprogramming 在开始的 8 天以内被开始并且独立于连续外长的 Oct4 表示。没有基因修正,这些发现将帮助 iPSCs 的未来产生,以及阐明位于 reprogramming 过程下面的分子的机制。 | Yanqin Li Qiang Zhang Xiaolei Yin Weifeng Yang Yuanyuan Du Pingping Hou Jian Ge Chun Liu Weiqi Zhang Xu Zhang Yetao Wu Honggang Li Kang Liu Chen Wu Zhihua Song Yang Zhao Yan Shi Hongkui Deng | 2011 | Cell Research2011，21，1： | 51 |
| 2 | Chemical characteristics and source apportionment of PM_(2.5) between heavily polluted days and other days in Zhengzhou, China显示文摘PM_(2.5) samples were collected in Zhengzhou during 3 years of observation, and chemical characteristics and source contribution were analyzed. Approximately 96% of the daily PM_(2.5) concentrations and annual average values exceeded the Chinese National Ambient Air Quality Daily and Annual Standards, indicating serious PM_(2.5) pollution. The average concentration of water-soluble inorganic ions was 2.4 times higher in heavily polluted days(daily PM32.5 concentrations > 250 μg/mand visibility < 3 km) than that in other days, with sulfate, nitrate, and ammonium as major ions. According to the ratio of NO-3/SO2-4,stationary sources are still the dominant source of PM_(2.5) and vehicle emission could not be ignored. The ratio of secondary organic carbon to organic carbon indicated that photochemical reactivity in heavily polluted days was more intense than in other days.Crustal elements were the most abundant elements, accounting for more than 60% of 23 elements. Chemical Mass Balance results indicated that the contributions of major sources(i.e., nitrate, sulfate, biomass, carbon and refractory material, coal combustion, soil dust,vehicle, and industry) of PM_(2.5) were 13%, 16%, 12%, 2%, 14%, 8%, 7%, and 8% in heavily polluted days and 20%, 18%, 9%, 2%, 27%, 14%, 15%, and 9% in other days, respectively.Extensive combustion activities were the main sources of polycyclic aromatic hydrocarbons during the episode(Jan 1-9, 2015) and the total benzo[a]pyrene equivalency concentrations in heavily polluted days present significant health threat. Because of the effect of regional transport, the pollution level of PM_(2.5) in the study area was aggravated. | Nan Jiang Qiang Li Fangcheng Su Qun Wang Xue Yu Panru Kang Ruiqin Zhang Xiaoyan Tang | 2018 | Journal of Environmental Sciences2018，30，4： | 22 |
| 3 | Role of PERK/eIF2α/CHOP Endoplasmic Reticulum Stress Pathway in Oxidized Low-density Lipoprotein Mediated Induction of Endothelial Apoptosis显示文摘Objective PERK/eI F2α/CHOP is a major signaling pathway mediating endoplasmic reticulum(ER) stress related with atherosclerosis.Oxidized LDL(ox-LDL) also induces endothelial apoptosis and plays a vital role in the initiation and progression of atherosclerosis.The present study was conducted to explore the regulatory effect of ox-LDL on PERK/e IF2α/CHOP signaling pathway in vascular endothelial cells.Methods The effects of ox-LDL on PERK and p-e IF2α protein expression of primary human umbilical vein endothelial cells(HUVECs) were investigated by Western blot analysis.PERK gene silencing and selective eI F2α phosphatase inhibitor,salubrinal were used to inhibit the process of ox-LDL induced endothelial cell apoptosis,caspase-3 activity,and CHOP mR NA level.Results Ox-LDL treatment significantly increased the expression of PERK,PERK-mediated inactivation of e IF2α phosphorylation,and the expression of CHOP,as well as the caspase-3 activity and apoptosis.The effects of ox-LDL were markedly decreased by knocking down PERK with stable transduction of lentiviral sh RNA or by selective eI F2α phosphatase inhibitor,salubrinal.Conclusion This study provides the first evidence that ox-LDL induces apoptosis in vascular endothelial cells mediated largely via the PERK/eI F2α/CHOP ER-stress pathway.It adds new insights into the molecular mechanisms underlying the pathogenesis and progression of atherosclerosis. | TAO Yong Kang YU Pu Lin BAI Yong Ping YAN Sheng Tao ZHAO Shui Ping ZHANG Guo Qiang | 2016 | Biomedical and Environmental Sciences2016，29，12： | 21 |
| 4 | Multicenter Evaluation of the Molecular Line Probe Assay for Multidrug Resistant Mycobacterium Tuberculosis Detection in China显示文摘In order to evaluate the performance of a molecular Hain line probe assay(Hain LPA)for rapid detection of rifampicin and isoniazid resistance of Mycobacterium tuberculosis in China。 | LI Qiang DONG Hai Yan PANG Yu XIA Hui OU Xi Chao ZHANG Zhi Ying LI Jun Chen ZHANG Jian Kang HUAN Shi Tong CHIN Daniel P KAM Kai Man ZHAO Yan Lin | 2015 | Biomedical and Environmental Sciences2015，28，6： | 13 |
| 5 | A DNA computer model for solving vertex coloring prob-lem显示文摘A special DNA computer was designed to solve the vertex coloring problem. The main body of this kind of DNA computer was polyacrylamide gel electrophoresis which could be classified into three parts: melting region, unsatisfied solution region and solution region. This polyacrylamide gel was con- nected with a controllable temperature device, and the relevant temperature was Tm1, Tm2 and Tm3, res- pectively. Furthermore, with emphasis on the encod- ing way, we succeeded in performing the experiment of a graph with 5 vertices. In this paper we introduce the basic structure, the principle and the method of forming the library DNA sequences. | XU Jin QIANG Xiaoli FANG Gang ZHOU Kang | 2006 | Chinese Science Bulletin2006，51，20： | 12 |
| 6 | A New Critical Nitrogen Dilution Curve for Rice Nitrogen Status Diagnosis in Northeast China显示文摘In-season diagnosis of crop nitrogen(N) status is crucial for precision N management. Critical N(N_c) dilution curve and N nutrition index(NNI) have been proposed as effective methods to diagnose N status of different crops. The N_c dilution curves have been developed for indica rice in the tropical and temperate zones and japonica rice in the subtropical-temperate zone, but they have not been evaluated for short-season japonica rice in Northeast China. The objectives of this study were to evaluate the previously developed N_c dilution curves for rice in Northeast China and to develop a more suitable N_c dilution curve in this region. A total of17 N rate experiments were conducted in Sanjiang Plain, Heilongjiang Province in Northeast China from 2008 to 2013. The results indicated that none of the two previously developed N_c dilution curves was suitable to diagnose N status of the short-season japonica rice in Northeast China. A new N_c dilution curve was developed and can be described by the equation N_c = 27.7 W^(-0.34) if W ≥ 1 Mg dry matter(DM) ha^(-1) or N_c = 27.7 g kg^(-1) DM if W < 1 Mg DM ha^(-1), where W is the aboveground biomass. This new curve was lower than the previous curves. It was validated using a separate dataset, and it could discriminate non-N-limiting and N-limiting nutritional conditions. Additional studies are needed to further evaluate it for diagnosing N status of different rice cultivars in Northeast China and develop efficient non-destructive methods to estimate NNI for practical applications. | HUANG Shanyu MIAO Yuxin CAO Qiang YAO Yinkun ZHAO Guangming YU Weifeng SHEN Jianning YU Kang Georg BARETH | 2018 | Pedosphere2018，28，5： | 10 |
| 7 | Effect of metabolic syndrome on prognosis and clinical characteristics of revascularization in patients with coronary artery disease显示文摘Background People with metabolic syndrome are at higher risk for developing coronary artery disease (CAD). The effect of the metabolic syndrome on outcomes in patients with preexisting CAD has not been well studied. This study was conducted to assess the prevalence, characteristics, in hospital and long term prognosis of CAD with metabolic syndrome and to determine the factors influencing the prognosis of the disease. Methods The DESIRE registry contains data of 3696 patients with CAD between 2001 and 2004. Mean long term followup was (829±373) days. Diagnosis of metabolic syndrome was based on modified International Diabetes Federation (IDF) Worldwide Definition of the Metabolic Syndrome,using body mass index (BMI) instead of waist circumference. Results Of 2596 patients with complete records of height, weight, and so on, 1280 (49.3%) were identified with metabolic syndrome. The patients with metabolic syndrome had higher level of body mass index, systolic blood pressure, diastolic blood pressure, fasting glucose and disordered blood lipid (all P<0.0001), with higher creatinine [(10.5±4.3) mg/L vs (9.9±2.9) mg/L, P<0.0001] and the number of white blood cells [(7.49±2.86) ×109/L vs (7.19±2.62) ×109/L, P=0.008) compared with those without metabolic syndrome. The patients with metabolic syndrome showed severer coronary angiographic alterations (left main artery and/or ≥2-vessel) (73.6% vs 69.6%, P=0.031). There were no significant differences of major adverse cardiac and cerebral events (MACCE) or mortality in hospital between the two groups. During followup, the ratio of MACCE in CAD with metabolic syndrome patients increased significantly (11.8% vs 10.0%, P=0.044). Fasting blood glucose (≥1000 mg/L) and triglyceride (TG, ≥1500 mg/L) were responsible for most of the increased risk associated with the metabolic syndrome (adjusted OR 1.465, 95% CI 1.037-1.874, P=0.032; OR 1.378, 95% CI 1.014-1.768, P=0.044). Conclusions The prevalence of metabolic syndrome was very high in CAD patients. The metabolic syndrome confers a higher risk of long term MACCE in patients with CAD, and dysglycaemia and hypertriglycaemia appear to be responsible for most of the associated risk. | HU Rong MA Chang-sheng NIE Shao-ping LU Qiang KANG Jun-ping DU Xin ZHANG Yin GAO Ying-chun HE Li-qun JIA Chang-qi LIU Xin-min DONG Jian-zeng LIU Xiao-hui CHEN Fang ZHOU Yu-jie LU Shu-zheng WU Xue-si | 2006 | Chinese Medical Journal2006，，22： | 9 |
| 8 | Twa1/Gid8 is a β-catenin nuclear retention factor in Wnt signaling and colorectal tumorigenesis显示文摘 | Yi Lu Shanshan Xie Wen Zhang Cheng Zhang Cheng Gao Qiang Sun Yuqi Cai Zhangqi Xu Min Xiao Yanjun Xu Xiao Huang Ximei Wu Wei Liu Fudi Wang Yibin Kang Tianhua Zhou | 2017 | Cell Research2017，27，12： | 9 |
| 9 | Microstructures and mechanical properties of directionally solidified multi-element Nb-Si alloy | Qiang HUANG Xi-ping GUO Yong-wang KANG Jin-xia SONG Shi-yu QU Ya-fang HAN | 2011 | Progress in Natural Science:Materials International2011，21，2： | 8 |
| 10 | Simultaneous determination of 20 food additives by high performance liquid chromatography with photo-diode array detector显示文摘An efficient and accurate analytical method was developed for the simultaneous determination of 20 synthetic food additives, including three sweeteners,seven food colorants,nine synthetic preservatives and caffeine,by high performance liquid chromatography (HPLC) with photodiode array detector(PDA).This method permits the detection of food additives at very low concentrations(0.005-0.150μg/mL).The applicability was verified by the determination of food additives present in various foodstuffs. | Kang Ma Ya Nan Yang Xiao Xiong Jiang Min Zhao Ye Qiang Cai | 2012 | Chinese Chemical Letters2012，23，4： | 8 |
| 11 | The effect of Y,Ce and Gd on texture,recrystallization and mechanical property of Mg-Zn alloys显示文摘The effect of Gd,Ce and Y elements on texture,recrystallization and mechanical properties of Mg–1.5Zn alloys was investigated.The results show that the addition of Gd,Ce and Y elements in Mg–1.5Zn alloy,which rolled at 450℃ and subsequently annealed at 350℃ for 1h,can effectively weaken and modify the basal texture,characterized by the splitting basal pole toward to transverse direction,leading to the yield and tensile strength,the highest along the rolling direction and the lowest along the transverse direction.Besides,the unique basal texture contributes to the significant improvement of elongation at room temperature.Electron back scattering diffraction(EBSD)analysis indicated that the non-basal texture in Mg–1.5Zn–0.2RE alloys can be attributed to obstructive effect of static recrystallization and the non-basal orientation grains nucleation near pre-existing grain boundaries during annealing.Specially,the Mg–1.5Zn–0.2Gd sheet exhibits much excellent plasticity with the elongation of 27%than Mg–1.5Zn–0.2Ce and Mg–1.5Zn–0.2Y alloys,resulting from the less and smaller second phase of MgZnGd. | Peng Liu Haitao Jiang Zhengxu Cai Qiang Kang Yun Zhang | 2016 | Journal of Magnesium and Alloys2016，4，3： | 8 |
| 12 | Microstructure evolution and mechanical property of Mg-3Al alloys with addition of Ca and Gd during rolling and annealing process显示文摘The formability of magnesium alloys at ambient temperature can be enhanced by alloying additions such as Ca and RE elements,which is ascribed to the weakened basal texture.To produce magnesium alloy sheets with excellent comprehensive performance,the evolution of texture characterization during fabrication process and subsequent effect of texture on mechanical properties are vital controlling factors.In this investigation,three experimental Mg-3Al series alloys were hot rolled and annealed to sheets with 1 mm thickness.The microstructure evolution during rolling and annealing process was investigated.Furthermore,the influence of texture on tensile properties along different tensile directions was also studied.The results show that weakened basal texture and refined grains were achieved with the co-addition of Ca and RE element.However,strengthening of mechanical properties wasn't obtained.During hot rolling process,microstructure was refined,second phase particles broken,and basal texture was increased.While basal texture was weakened during annealing process.Recrystallization behavior influenced by formation of second phase was dominated to attenuate basal texture.Tensile deformation behavior was controlled by basal slip and followed Schmid factor criterion.Moreover,the weakened basal texture and activation of non-basal slips during hot rolling process can contribute to diminish the anisotropy of tensile properties. | Yun Zhang Haitao Jiang Qiang Kang Yujiao Wang Yonggang Yang Shiwei Tian | 2020 | Journal of Magnesium and Alloys2020，8，3： | 7 |
| 13 | Kinetics and Mechanism of Interfacial Reaction in a SiC_f/Ti Composite显示文摘In order to evaluate the interfacial reaction, a SiCf/Ti (TA1) composite was fabricated by a vacuum hot pressingmethod and then heat-treated in vacuum at 800℃ for up to 100 h. The elemental distributions of C, Si and Ti at theinterfacial reaction zone were investigated. It was found that the reaction zone occurs during the fabrication processand continuously grows at high temperature because the Si and C atoms diffuse from SiC fibers to the matrix and Tiatoms diffuse in the opposite direction. The growth of the reaction zone is diffusion controlled and the mechanismof the reaction can be described by a reactive diffusion model of solid-state growth of an AmBn layer between twoelementary substances A and B. | Guoxing ZHANG, Qiang KANG, Nanlin SHI, Geping LI and Dong LIInstitute of Metal Research, Chinese Academy of Sciences, Shenyang 110016, China | 2003 | Journal of Materials Science & Technology2003，19，5： | 7 |
| 14 | 17β-estradiol promotes recovery after myocardial infarction by enhancing homing and angiogenic capacity of bone marrow-derived endothelial progenitor cells through ERa-SDF-1/ CXCR4 crosstalking显示文摘17β-estradiol (E2)has been shown to mediate endothelial progenitor cells (EPCs)to repair infarcted myocardium.Both.estrogen receptor α(ERα)and stromal derived factor-1 (SDF-1)/CXCR4 signaling pathways may play a critical role in regulating homing and angiogenesis of EPCs in this process.However,the interaction between ERa and SDF-1/CXCR4 signaling pathways remains unclear.In response to E2,the expression of SDF-1 and CXCR4 in EPCs from ovariectomized BALB/C mice was obviously up-regulated,in addition,the migration and tube formation of EPCs in vitro were also significantly enhanced.However,ERα antagonist (MMP)and CXCR4 inhibitor (AMD3100)significantly decreased the migration The combined treatment of MMP and AMD3100 and tube length of EPCs,even if mediated by E2. exerted more inhibitory effects on migration and tube formation of EPCs induced by E2.In in vivo studies,ovariectomized mice were induced acute myocardial infarction (AMI),and divided into four groups (n =6):non-preconditioned EPCs (3 × 10^6)group,E2-preconditioned EPCs group,MMP +AMD3100 preconditioned EPCs group,and EPCs pretreated with E2 +MMP +AMD3100 group.E2 group displayed a greater number of homing EPCs,increased capillary density in infarcted myocardium,decreased left ventricular (LV) fibrosis.Nevertheless,these effects of E2 were almost completely blocked by the combined treatment of MMP and AMD3100.E2 can produce cardiovascular protective effects in AMI setting by enhancing homing and angiogenic capacity of EPCs through ERα and CXCR4 signaling pathways, which means that ERα and CXCR4 pathways are effective targets for the development of treatment strategies for AMI. | Zhize Yuan Lei Kang Zhe Wang Anqing Chen Qiang Zhao Haiqing Li | 2018 | Acta Biochimica et Biophysica Sinica2018，50，12： | 6 |
| 15 | Texture and stretch formability of rolled Mg–Zn–RE(Y, Ce, and Gd) alloys at room temperature显示文摘To develop a new magnesium alloy with excellent formability at room temperature,the effect of Y,Ce,and Gd addition on texture and stretch formability of Mg–1.5Zn alloys was carried out.The result shows that Y,Ce,and Gd addition in Mg–1.5Zn alloys can effectively weaken and modify the basal plane texture,characterized by TD-split texture in which the position of basal is titled from normal direction(ND)toward transverse direction(TD).When Mg–1.5Zn alloy with Gd addition appears low texture intensity and TD-split texture,where the position of basal poles is tilted by about±35°from ND toward to TD,the largest Erichsen value of 7.0 and the elongation rate reaches 29.1%in TD direction.However,Y and Ce addition in Mg–1.5Zn alloys promote a large number of second phase particles,which cancel the contribution of the unique basal texture to stretch formability and ductility. | Zheng-Xu Cai Hai-Tao Jiang Di Tang Zhao Ma Qiang Kang | 2013 | Rare Metals2013，32，5： | 6 |
| 16 | Understanding the lifestyles and pathogenicity mechanisms of obligate biotrophic fungi in wheat:The emerging genomics era显示文摘Obligate biotrophic fungi cause serious and widespread diseases of crop plants, but are challenging to investigate because they cannot be cultured in vitro. The two economically important groups of biotrophic fungi parasitizing wheat are the rust and powdery mildew pathogens, but their obligate biotrophic lifestyles and pathogenicity mechanisms are not well understood at the molecular level. With the advent of next generation sequencing technology, increasing numbers of pathogen genomes are becoming available. Research in plant pathology has entered a new genomics era. This review summarizes recent progress in understanding the biology and pathogenesis of biotrophic fungal pathogens attacking wheat based on pathogen genomics. We particularly focus on the three wheat rust and the powdery mildew fungi in regard to genome sequencing, avirulence gene cloning, effector discovery, and pathogenomics. We predict that coordinated study of both wheat and its pathogens should reveal new insights in biotrophic adaptation, pathogenicity mechanisms,and population dynamics of these fungi that will assist in development of new strategies for breeding wheat varieties with durable resistance. | Chunlei Tang Qiang Xu Mengxin Zhao Xiaojie Wang Zhensheng Kang | 2018 | The Crop Journal2018，6，1： | 6 |
| 17 | A novel diffusion model considering curvature radius at the bonding interface in a titanium/steel explosive clad plate显示文摘This article introduces an element diffusion behavior model for a titanium/steel explosive clad plate characterized by a typical curved interface during the heat-treatment process. A series of heat-treatment experiments were conducted in the temperature range from 750°C to 950°C, and the effects of heat-treatment parameters on the microstructural evolution and diffusion behavior were investigated by optical microscopy, scanning electron microscopy, X-ray diffraction analysis, and electron-probe microanalysis. Carbon atoms within the steel matrix were observed to diffuse toward the titanium matrix and to aggregate at the bonding interface at 850°C or lower; in contrast, when the temperature exceeded 850°C, the mutual diffusion of Ti and Fe occurred, along with the diffusion of C atoms, resulting in the formation of Ti–Fe intermetallics(Fe2Ti/FeTi). The diffusion distances of C, Ti, and Fe atoms increased with increasing heating temperature and/or holding time. On the basis of this diffusion behavior, a novel diffusion model was proposed. This model considers the effects of various factors, including the curvature radius of the curved interface, the diffusion coefficient, the heating temperature, and the holding time. The experimental results show good agreement with the calculated values. The proposed model could clearly provide a general prediction of the elements' diffusion at both straight and curved interfaces. | Hai-tao Jiang Qiang Kang Xiao-qian Yan | 2015 | International Journal of Minerals,Metallurgy and Materials2015，22，9： | 5 |
| 18 | HSP70 decreases receptor-dependent phosphorylation of Smad2 and blocks TGF-β-induced epithelial-mesenchymal transition显示文摘Smad2 和 Smad3,转变发信号的生长因素(TGF-) 的细胞内部的调停人,直接由激活的类型的 phosphorylated 我从进原子核到的细胞质的受体 kinase,然后梭调整目标基因表示。这里,我们报导 70-kDa 热吃惊蛋白质(HSP70 ) 与 Smad2 和减少 TGF- 信号 transduction 交往。HSP70 的宫外的表示阻止受体依赖者 phosphorylation 和 Smad2 的原子 translocation,并且堵住 TGF -- 在 HaCat 房间的导致的上皮间充质的转变(EMT ) 。我们的调查结果在 TGF 揭示 HSP70 的一个必要角色 -- 由阻碍 Smad2 phosphorylation 的导致的上皮间充质的转变(EMT ) 。 | Yihao Li Xianjiang Kang Qiang Wang | 2011 | Journal of Genetics and Genomics2011，38，3： | 5 |
| 19 | Good laboratory practices guarantee biosafety in the Sierra Leone-China friendship biosafety laboratory显示文摘Background:The outbreak of Ebola virus disease(EVD)in West Africa between 2014 and 2015 was the largest EDV epidemic since the identification of Ebola virus(EBOV)in 1976,and the countries most strongly affected were Sierra Leone,Guinea,and Liberia.Findings:The Sierra Leone-China Friendship Biological Safety Laboratory(SLE-CHN Biosafety Lab),a fixed Biosafety Level 3 laboratory in the capital city of Sierra Leone,was established by the Chinese government and has been active in EBOV detection since 11 March 2015.Complete management and program documents were created for the SLE-CHN Biosafety Lab,and it was divided into four zones(the green,yellow,brown,and red zones)based on the risk assessment.Different types of safe and appropriate personnel protection equipment(PPE)are used in different zones of the laboratory,and it fully meets the Biosafety Level 3 laboratory standards of the World Health Organization.Conclusion:Good preparedness,comprehensive risk assessment and operation documents,appropriate PPE,effective monitoring and intensive training,together with well-designed and reasonable laboratory sectioning are essential for guaranteeing biosafety. | Qin Wang Wei-Min Zhou Yong Zhang Huan-Yu Wang Hai-Jun Du Kai Nie Jing-Dong Song Kang Xiao Wen-Wen Lei Jian-Qiang Guo He-Jiang Wei Kun Cai Yan-Hai Wang Jiang Wu Gerard Kamara Idrissa Kamara Qiang Wei Mi-Fang Liang Gui-Zhen Wu Xiao-Ping Dong | 2016 | Infectious Diseases of Poverty2016，5，1： | 5 |
| 20 | Elevated Platelet Activating Factor Level in Ischemia-Related Arrhythmia and Its Electrophysiological Effect on Myocardium显示文摘Objective The mechanism through which platelet activating factor (PAF) induces cardiac electrical activity and arrhythmia is not well understood and previous studies have suggested a potential involvement of ion channels in its action. The present study was aimed to clarify the role of PAF in fatal arrhythmias following acute myocardia infarction (AMI) and the underlying mechanism. Methods (1) Blood PAF levels were measured among 72 AMI patients at the time of diagnosis with AMI and 48 h later, and their electrocardiogram (ECG) was recorded continuously. (2) Ischemia simulation and surface electrocardiogram were conducted in 20 pigs and their PAF levels were measured. (3) PAF perfusion and standard microelectrode recording were performed on guinea pig papillarymuscles. Results In both humans and pigs, elevated PAF levels were detected in AMI and simulated ischemia, respectively, and even higher PAF levels were found when fatal arrhythmias occurred. In guinea pig myocardium, PAF induced a shortening of action potential duration at 90% level of repolarization (APD 90 )under non-ischemic conditions and a more pronounced shortening under early simulated ischemic conditions. Conclusion AMI and ischemia are associated with increased PAF levels in humans and pigs, which are further raised when fatal arrhythmia follows. The effects of PAF on the myocardium may be mediated by multiple ion channels. | TAO Yong Kang ZHAO Shui Ping YU Pu Lin SHI Jing GU Cheng Dong SUN Hong Tao ZHANG Guo Qiang | 2013 | Biomedical and Environmental Sciences2013，26，5： | 5 |
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